WO2022227354A1 - Experimental container and biological sample preparation device - Google Patents

Experimental container and biological sample preparation device Download PDF

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Publication number
WO2022227354A1
WO2022227354A1 PCT/CN2021/114998 CN2021114998W WO2022227354A1 WO 2022227354 A1 WO2022227354 A1 WO 2022227354A1 CN 2021114998 W CN2021114998 W CN 2021114998W WO 2022227354 A1 WO2022227354 A1 WO 2022227354A1
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WO
WIPO (PCT)
Prior art keywords
teeth
cover
experimental container
row
guide tube
Prior art date
Application number
PCT/CN2021/114998
Other languages
French (fr)
Chinese (zh)
Inventor
刘南峰
阳方义
潘雅楠
Original Assignee
深圳市瑞沃德生命科技有限公司
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Filing date
Publication date
Application filed by 深圳市瑞沃德生命科技有限公司 filed Critical 深圳市瑞沃德生命科技有限公司
Publication of WO2022227354A1 publication Critical patent/WO2022227354A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving

Definitions

  • the present application relates to the technical field of experimental instruments, and in particular, to an experimental container and a biological sample preparation device.
  • Tissue is a cellular structure bounded between cells and organs, and consists of many cells with similar shapes and intercellular substance.
  • tissues need to be processed to prepare single-cell suspensions.
  • a single cell suspension refers to a biological sample in which discrete single cells are released due to disruption of the connections between cells in the tissue.
  • the prepared single cell suspension needs to have the characteristics of sufficient cell dispersion and high activity, that is, it is necessary to fully destroy the connection between cells while maintaining the integrity and activity of single cells, which is the basis for cell research.
  • the structure of the container and/or device for preparing the single cell suspension Based on the above characteristics of the single cell suspension, higher requirements are placed on the structure of the container and/or device for preparing the single cell suspension.
  • the existing device is used to treat tissues with strong adhesion, such as mouse brain tissue, the cell dispersion in the prepared single cell suspension is not ideal.
  • the embodiment of the present application provides an experimental container, which aims to solve the problem of insufficient dispersion of cells in a single cell suspension in the prior art.
  • an experimental container including:
  • a container body (1) having an open end (11) and a closed end (12);
  • the processing assembly (3) comprises a guide tube (31) rotatable relative to the first cover (2) through the first cover (2), at least a A row of teeth (32) and a stirring element (33) fixed on the guide tube (31);
  • the stirring element (33) comprises at least one cantilevered cutting blade (331) and a structural part (332) matched with at least one row of teeth (32), the structural part (332) extends from the guide pipe (31) and covers the on at least one row of teeth (32).
  • a biological sample preparation device including a housing, a motor, and an experimental container as described above.
  • the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon.
  • the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
  • Fig. 1 is the exploded view of the experiment container provided by the first embodiment of the present application
  • FIG. 2 is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application;
  • FIG. 3 is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application;
  • Fig. 4 is the schematic diagram of B area among Fig. 3;
  • FIG. 5 is a schematic structural diagram of the biological sample preparation device provided in the third embodiment of the present application.
  • the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon.
  • the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
  • FIG. 1 is an exploded view of the experimental container provided in the first embodiment of the present application.
  • the shape of the experimental container is similar to that of a test tube, and includes a container body 1 , a first cover 2 and a processing assembly 3 .
  • the container body 1 has an open end 11 and a closed end 12 .
  • a partial area of the outer surface of the open end 11 is provided with an external thread, which can cooperate with the internal thread on the first cover 2 to connect and fix the first cover 2 .
  • the processing assembly 3 is provided on the first cover 2 and includes a guide tube 31 , at least one row of teeth 32 and a stirring element 33 .
  • the guide tube 31 passes through the first cover 2 through a central hole (not shown in the figure) of the first cover 2 , and is rotatable relative to the first cover 2 .
  • the three rows of teeth 32 are arranged around the guide tube 31 , are at different levels, and are fixed on the first cover 2 .
  • the stirring element 33 is fixed on the upper outer surface of the guide tube 31 , extends downward, and is rotatable relative to the three rows of teeth 32 .
  • the stirring element 33 includes a cantilevered cutting blade 331 and a structural member 332 matched with the three rows of teeth 32 .
  • a plurality of cantilever cutters 331 may also be provided.
  • the structural member 332 extends from the guide tube 31 and covers the three rows of teeth 32 , and the distance between the corresponding surfaces of the structural member 332 and the three rows of teeth 32 is relatively short. According to the requirements of the particle size of the biological sample to be prepared, the distance is preferably about 100um when preparing a single-cell suspension, and when preparing a homogenate (a biological sample that destroys the cell structure, releasing the protein inside the cell and accounting), the distance is Preferably it is about 20um.
  • the lower inner surface of the guide tube 31 includes a joint (not shown in the figure) for connecting the external drive shaft.
  • the artificially chopped tissue is put into the experimental container, the external drive shaft is connected to the joint of the guide tube 31, and the external drive shaft rotates to drive the guide tube 31 and the stirring element 33 to rotate, the container body 1, The first cover 2 and the three rows of teeth 32 fixed on the first cover 2 do not move, and the tissue in the experimental container is cut by the cantilever cutter 331 and then subjected to grinding by the structural member 332, and the cycle is processed in this way.
  • the rotation speed and processing time are comprehensively set according to the adhesion of the tissue and the particle size of the biological sample to be prepared.
  • the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon.
  • the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
  • the basic structure of the experimental container is the same as that of the first embodiment, and the same reference numerals are used for the same components as those of the first embodiment, including all the features described in the first embodiment, which will not be repeated here. Repeat.
  • FIG. 2 is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application.
  • FIG. 3 is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application.
  • the structural member 332 further includes a spiral baffle plate 3321 forming a predetermined angle with the three rows of teeth 32 .
  • the structural member 332 is rotated, the tissue to be treated is pushed toward the cantilevered cutting blade 331 by the helical baffle 3321 and subjected to cutting by the cantilevered cutting blade 331 .
  • the baffle 3321 is set in a helical shape, which can reduce the mechanical resistance of the tissue to be treated when the structural member 332 is rotated.
  • at least one pressure relief port A is opened on the edge of the spiral baffle 3321, and the pressure relief port A is a slit with a narrow width, allowing the biological samples that have been processed into small particles to pass through, further reducing the impact of the structural member 332 during rotation. mechanical resistance of the tissue to be treated.
  • FIG. 4 is a schematic diagram of area B in FIG. 3 .
  • the cross section of the cantilevered cutting blade 331 in the vertical direction is fan-shaped, and both ends are cutting edges, which can cut the tissue to be treated that is contacted during rotation.
  • the edge of the cantilevered cutting knife 331 in the vertical direction facing the middle row of teeth and the outer circumference of the middle row of teeth form a triangle-like shape, that is, d1 shown in FIG. 4 is greater than d2 , but still maintains a certain narrow distance at the narrowest part, so that the cut tissue to be treated can pass through, and it can be squeezed and ground to a certain extent when passing through.
  • the cantilevered cutting knife 331 realizes the rough cutting of the tissue to be treated
  • the structural member 332 realizes the fine grinding of the tissue to be treated
  • the two processes of rough cutting and fine grinding are completed by two different components, so that the prepared biological sample is Cells are more fully dispersed.
  • the structural member 332 and the cantilevered cutting blade 331 are symmetrically distributed with respect to the guide tube 31, and can also be set at an included angle of 90 degrees, 45 degrees, or the like.
  • the experimental container further includes an absorption ring 5 and a second cover 6 for preventing leakage.
  • the absorption ring 5 is sleeved on the lower part of the guide tube 31 and accommodated in the second cover 6 .
  • the second cover 6 is fixed on the first cover 2 and can also be used as a container for containing leaked liquid.
  • the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon.
  • the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
  • FIG. 5 is a schematic structural diagram of the biological sample preparation device provided in the third embodiment of the present application. 1 and 5 , the biological sample preparation device includes a housing, a motor, and an experimental container as described in Embodiment 1 or Embodiment 2.
  • the motor is arranged in the casing, the experimental container is placed in the notch of the casing, the container body 1 and the first cover 2 are fixed, and the drive shaft of the motor is connected with the guide tube 31 to drive the experimental container to rotate to prepare biological samples.
  • the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon.
  • the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

An experimental container, comprising: a container body (1); a first cover (2); and a processing assembly (3) provided on the first cover (2). The processing assembly (3) comprises a guide tube (31) that penetrates through the first cover (2) and is rotatable with respect to the first cover (2), at least one row of teeth (32) fixed on the first cover (2) and arranged around the guide tube (31), and a stirring element (33) fixed on the guide tube (31). The stirring element (33) comprises at least one cantilever-type cutting knife (331) and a structural member (332) matching the at least one row of teeth (32). The structural member (332) extends from the guide tube (31) and covers the at least one row of teeth (32). The tissue put in the experimental container is cut by means of the rotatable cantilever-type cutting knife (331), and is ground by means of a plurality of rows of teeth (32) fixed on the first cover (2) and the rotatable structural member (332) covering the teeth. When the cantilever-type cutting knife (331) and the structural member (332) rotate, the tissue in the experimental container is repeatedly cut and ground, such that cells in the prepared biological sample are dispersed more sufficiently.

Description

一种实验容器及生物样本制备装置An experimental container and biological sample preparation device
本申请要求于2021年4月29日在中国专利局提交的、申请号为202110473378.3的中国专利申请的优先权，其全部内容通过引用结合在本申请中。This application claims the priority of the Chinese Patent Application No. 202110473378.3 filed with the Chinese Patent Office on April 29, 2021, the entire contents of which are incorporated herein by reference.
技术领域technical field
本申请涉及实验仪器技术领域，尤其涉及一种实验容器及生物样本制备装置。The present application relates to the technical field of experimental instruments, and in particular, to an experimental container and a biological sample preparation device.
背景技术Background technique
组织是界于细胞和器官之间的细胞架构，由许多形态相似的细胞及细胞间质组成。为了对生物体进行细胞研究，需要对组织进行处理制备成单细胞悬液。单细胞悬液是指因组织中细胞之间的连接遭到破坏而释放出分散的单个细胞的一种生物样本。制备得到的单细胞悬液需具备细胞分散充分、活性高等特点，即需充分破坏细胞之间的连接而又保持单个细胞的完整和活性，是进行细胞研究的基础。Tissue is a cellular structure bounded between cells and organs, and consists of many cells with similar shapes and intercellular substance. For cellular studies of organisms, tissues need to be processed to prepare single-cell suspensions. A single cell suspension refers to a biological sample in which discrete single cells are released due to disruption of the connections between cells in the tissue. The prepared single cell suspension needs to have the characteristics of sufficient cell dispersion and high activity, that is, it is necessary to fully destroy the connection between cells while maintaining the integrity and activity of single cells, which is the basis for cell research.
基于单细胞悬液的上述特点，对制备单细胞悬液的容器和/或装置的结构提出了较高的要求。现有的装置在处理黏连性较强的组织时，如小鼠脑组织，制备得到的单细胞悬液中细胞分散性还不理想。Based on the above characteristics of the single cell suspension, higher requirements are placed on the structure of the container and/or device for preparing the single cell suspension. When the existing device is used to treat tissues with strong adhesion, such as mouse brain tissue, the cell dispersion in the prepared single cell suspension is not ideal.
技术问题technical problem
本申请实施例提供一种实验容器，旨在解决现有技术中单细胞悬液中细胞分散不充分的问题。The embodiment of the present application provides an experimental container, which aims to solve the problem of insufficient dispersion of cells in a single cell suspension in the prior art.
技术解决方案technical solutions
第一方面，提供了一种实验容器，包括：In a first aspect, an experimental container is provided, including:
具有开口端（11）和封闭端（12）的容器本体（1）；a container body (1) having an open end (11) and a closed end (12);
连接到容器本体（1）开口端（11）的第一盖（2）；以及a first cover (2) connected to the open end (11) of the container body (1); and
设置在第一盖（2）上的处理组件（3）；a processing assembly (3) arranged on the first cover (2);
其中，处理组件（3）包括穿过第一盖（2）相对第一盖（2）可旋转的引导管（31）、固定在第一盖（2）上围绕引导管（31）布置的至少一排齿（32）和固定在引导管（31）上的搅拌元件（33）；Wherein, the processing assembly (3) comprises a guide tube (31) rotatable relative to the first cover (2) through the first cover (2), at least a A row of teeth (32) and a stirring element (33) fixed on the guide tube (31);
其中，搅拌元件（33）包括至少一个悬臂式切割刀（331）和与至少一排齿（32）相配合的结构件（332），结构件（332）从引导管（31）延伸并覆盖于至少一排齿（32）上。Wherein, the stirring element (33) comprises at least one cantilevered cutting blade (331) and a structural part (332) matched with at least one row of teeth (32), the structural part (332) extends from the guide pipe (31) and covers the on at least one row of teeth (32).
第二方面，提供了一种生物样本制备装置，包括外壳、电机和如上所述的实验容器。In a second aspect, a biological sample preparation device is provided, including a housing, a motor, and an experimental container as described above.
有益效果beneficial effect
本申请实施例通过可旋转的悬臂式切割刀对放入实验容器中的组织进行切割，通过固定在第一盖上的多排齿和覆盖于其上的可旋转的结构件对放入实验容器中的组织进行碾磨，当悬臂式切割刀和结构件旋转时，实验容器中的组织反复地经受切割和碾磨处理，使得制备得到的生物样本中细胞分散更为充分。In the embodiment of the present application, the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon. When the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
附图说明Description of drawings
本申请上述的和/或附加的方面和优点从下面结合附图对实施例的描述中将变得明显和容易理解，其中：The above and/or additional aspects and advantages of the present application will become apparent and readily understood from the following description of embodiments taken in conjunction with the accompanying drawings, wherein:
图1是本申请实施例一提供的实验容器的***图；Fig. 1 is the exploded view of the experiment container provided by the first embodiment of the present application;
图2是本申请实施例二提供的第一盖2和处理组件3的侧视图；FIG. 2 is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application;
图3是本申请实施例二提供的第一盖2和处理组件3的俯视图；FIG. 3 is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application;
图4是图3中B区域的示意图；Fig. 4 is the schematic diagram of B area among Fig. 3;
图5是本申请实施例三提供的生物样本制备装置的结构示意图。FIG. 5 is a schematic structural diagram of the biological sample preparation device provided in the third embodiment of the present application.
本发明的实施方式Embodiments of the present invention
下面详细描述本申请的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的模块或具有相同或类似功能的模块。下面通过参考附图描述的实施例是示例性的，仅用于解释本申请，而不能理解为对本申请的限制。相反，本申请的实施例包括落入所附加权利要求书的精神和内涵范围内的所有变化、修改和等同物。The following describes in detail the embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar modules or modules having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, but should not be construed as a limitation on the present application. On the contrary, the embodiments of the present application include all changes, modifications and equivalents falling within the spirit and scope of the appended claims.
本申请实施例通过可旋转的悬臂式切割刀对放入实验容器中的组织进行切割，通过固定在第一盖上的多排齿和覆盖于其上的可旋转的结构件对放入实验容器中的组织进行碾磨，当悬臂式切割刀和结构件旋转时，实验容器中的组织反复地经受切割和碾磨处理，使得制备得到的生物样本中细胞分散更为充分。In the embodiment of the present application, the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon. When the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
实施例一Example 1
图1是本申请实施例一提供的实验容器的***图。如图1所示，该实验容器的形状与试管相似，包括容器本体1、第一盖2和处理组件3。容器本体1具有开口端11和封闭端12。开口端11外表面的局部区域设置了外螺纹，可与第一盖2上的内螺纹配合，连接、固定第一盖2。处理组件3设置在第一盖2上，包括引导管31、至少一排齿32和搅拌元件33。FIG. 1 is an exploded view of the experimental container provided in the first embodiment of the present application. As shown in FIG. 1 , the shape of the experimental container is similar to that of a test tube, and includes a container body 1 , a first cover 2 and a processing assembly 3 . The container body 1 has an open end 11 and a closed end 12 . A partial area of the outer surface of the open end 11 is provided with an external thread, which can cooperate with the internal thread on the first cover 2 to connect and fix the first cover 2 . The processing assembly 3 is provided on the first cover 2 and includes a guide tube 31 , at least one row of teeth 32 and a stirring element 33 .
引导管31通过第一盖2的中心孔（图中未示出）穿过第一盖2，相对第一盖2可旋转。在本申请实施例中，三排齿32围绕引导管31布置，处于不同的水平高度，固定在第一盖2上。搅拌元件33固定于引导管31的上部外表面，并往下延伸，相对三排齿32可旋转。其中，搅拌元件33包括一个悬臂式切割刀331和与三排齿32相配合的结构件332。作为本申请的实施例，悬臂式切割刀331也可以设置为多个。结构件332从引导管31延伸并覆盖于三排齿32上，结构件332与三排齿32之间对应表面的距离比较短。根据所需制备的生物样本颗粒度的要求，制备单细胞悬液时该距离优选为100um左右，制备匀浆（破坏了细胞结构的生物样本，释放出细胞内部的蛋白与核算）时，该距离优选为20um左右。引导管31的下部内表面包括结合部（图中未示出），用于连接外部驱动轴。使用该实验容器处理组织时，将人工切碎的组织放入实验容器，外部驱动轴与引导管31的结合部连接，外部驱动轴旋转时带动引导管31和搅拌元件33旋转，容器本体1、第一盖2以及固定在第一盖2上的三排齿32不动，实验容器内的组织经受悬臂式切割刀331的切割后，再经受结构件332的碾磨，如此循环处理。旋转速度和处理时间视组织的黏连性、所需制备的生物样本颗粒度的要求综合设置。The guide tube 31 passes through the first cover 2 through a central hole (not shown in the figure) of the first cover 2 , and is rotatable relative to the first cover 2 . In the embodiment of the present application, the three rows of teeth 32 are arranged around the guide tube 31 , are at different levels, and are fixed on the first cover 2 . The stirring element 33 is fixed on the upper outer surface of the guide tube 31 , extends downward, and is rotatable relative to the three rows of teeth 32 . The stirring element 33 includes a cantilevered cutting blade 331 and a structural member 332 matched with the three rows of teeth 32 . As an embodiment of the present application, a plurality of cantilever cutters 331 may also be provided. The structural member 332 extends from the guide tube 31 and covers the three rows of teeth 32 , and the distance between the corresponding surfaces of the structural member 332 and the three rows of teeth 32 is relatively short. According to the requirements of the particle size of the biological sample to be prepared, the distance is preferably about 100um when preparing a single-cell suspension, and when preparing a homogenate (a biological sample that destroys the cell structure, releasing the protein inside the cell and accounting), the distance is Preferably it is about 20um. The lower inner surface of the guide tube 31 includes a joint (not shown in the figure) for connecting the external drive shaft. When using the experimental container to process the tissue, the artificially chopped tissue is put into the experimental container, the external drive shaft is connected to the joint of the guide tube 31, and the external drive shaft rotates to drive the guide tube 31 and the stirring element 33 to rotate, the container body 1, The first cover 2 and the three rows of teeth 32 fixed on the first cover 2 do not move, and the tissue in the experimental container is cut by the cantilever cutter 331 and then subjected to grinding by the structural member 332, and the cycle is processed in this way. The rotation speed and processing time are comprehensively set according to the adhesion of the tissue and the particle size of the biological sample to be prepared.
本申请实施例通过可旋转的悬臂式切割刀对放入实验容器中的组织进行切割，通过固定在第一盖上的多排齿和覆盖于其上的可旋转的结构件对放入实验容器中的组织进行碾磨，当悬臂式切割刀和结构件旋转时，实验容器中的组织反复地经受切割和碾磨处理，使得制备得到的生物样本中细胞分散更为充分。In the embodiment of the present application, the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon. When the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
实施例二Embodiment 2
在本申请实施例中，实验容器的基本结构与实施例一相同，与实施例一相同的各部件沿用与实施例一相同的标号，包括在实施例一中描述的全部特征，在此不再赘述。In the embodiment of the present application, the basic structure of the experimental container is the same as that of the first embodiment, and the same reference numerals are used for the same components as those of the first embodiment, including all the features described in the first embodiment, which will not be repeated here. Repeat.
图2是本申请实施例二提供的第一盖2和处理组件3的侧视图。图3是本申请实施例二提供的第一盖2和处理组件3的俯视图。如图2和图3所示，在本申请实施例中，结构件332还包括与三排齿32形成预设夹角的螺线形挡板3321。结构件332旋转时，待处理的组织被螺线形挡板3321推向悬臂式切割刀331，经受悬臂式切割刀331的切割。挡板3321设置为螺线形，可降低结构件332在旋转时受到的待处理组织的机械阻力。优选地，在螺线形挡板3321边缘开设至少一个泄压口A，泄压口A为宽度较窄的缝，允许已被处理成小颗粒的生物样本通过，进一步降低结构件332在旋转时受到的待处理组织的机械阻力。FIG. 2 is a side view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application. FIG. 3 is a top view of the first cover 2 and the processing assembly 3 provided in the second embodiment of the present application. As shown in FIG. 2 and FIG. 3 , in the embodiment of the present application, the structural member 332 further includes a spiral baffle plate 3321 forming a predetermined angle with the three rows of teeth 32 . When the structural member 332 is rotated, the tissue to be treated is pushed toward the cantilevered cutting blade 331 by the helical baffle 3321 and subjected to cutting by the cantilevered cutting blade 331 . The baffle 3321 is set in a helical shape, which can reduce the mechanical resistance of the tissue to be treated when the structural member 332 is rotated. Preferably, at least one pressure relief port A is opened on the edge of the spiral baffle 3321, and the pressure relief port A is a slit with a narrow width, allowing the biological samples that have been processed into small particles to pass through, further reducing the impact of the structural member 332 during rotation. mechanical resistance of the tissue to be treated.
悬臂式切割刀331的一端固定于引导管31，然后向外延伸，垂直方向为两端均具有刃口的切割刀，切割刀的末端延伸到三排齿32上方，优选地，延伸到中间一排齿与最下方一排齿之间的台阶上。图4是图3中B区域的示意图。如图4所示，悬臂式切割刀331垂直方向上的切割刀的横截面为扇形，两端均为刃口，可切割旋转中接触到的待处理组织。对于同一横截面，悬臂式切割刀331垂直方向上的切割刀朝向中间一排齿的边与中间一排齿的外周（以圆弧示意）形成类三角形，即图4中所示的d1大于d2，但最窄处仍然保持一定的较窄距离，使得被切割后的待处理组织可以通过，而且通过时可对其进行一定程度的挤压和碾磨。One end of the cantilevered cutting knife 331 is fixed to the guide tube 31, and then extends outward. The vertical direction is a cutting knife with cutting edges at both ends. The end of the cutting knife extends above the three rows of teeth 32, preferably, extends to the middle one. on the step between the row of teeth and the bottom row of teeth. FIG. 4 is a schematic diagram of area B in FIG. 3 . As shown in FIG. 4 , the cross section of the cantilevered cutting blade 331 in the vertical direction is fan-shaped, and both ends are cutting edges, which can cut the tissue to be treated that is contacted during rotation. For the same cross section, the edge of the cantilevered cutting knife 331 in the vertical direction facing the middle row of teeth and the outer circumference of the middle row of teeth (shown as arcs) form a triangle-like shape, that is, d1 shown in FIG. 4 is greater than d2 , but still maintains a certain narrow distance at the narrowest part, so that the cut tissue to be treated can pass through, and it can be squeezed and ground to a certain extent when passing through.
悬臂式切割刀331实现对待处理组织的粗切，结构件332实现对待处理组织的细磨，并且粗切和细磨这两种处理由两个不同的组件来完成，使得制备得到的生物样本中细胞分散更为充分。The cantilevered cutting knife 331 realizes the rough cutting of the tissue to be treated, the structural member 332 realizes the fine grinding of the tissue to be treated, and the two processes of rough cutting and fine grinding are completed by two different components, so that the prepared biological sample is Cells are more fully dispersed.
作为本申请的实施例，从俯视图的角度观察，结构件332和悬臂式切割刀331关于引导管31对称分布，也可设置为夹角90度、45度等。As an embodiment of the present application, from the perspective of the top view, the structural member 332 and the cantilevered cutting blade 331 are symmetrically distributed with respect to the guide tube 31, and can also be set at an included angle of 90 degrees, 45 degrees, or the like.
为了防止漏液，引导管31和三排齿32通过密封件4连接。进一步地，如图1所示，实验容器还包括用于防泄漏的吸收环5和第二盖6。吸收环5套接在引导管31的下部，并容纳于第二盖6中。第二盖6固定在第一盖2上，同时也可作为容纳泄漏液体的容器。In order to prevent liquid leakage, the guide tube 31 and the three rows of teeth 32 are connected by the sealing member 4 . Further, as shown in FIG. 1 , the experimental container further includes an absorption ring 5 and a second cover 6 for preventing leakage. The absorption ring 5 is sleeved on the lower part of the guide tube 31 and accommodated in the second cover 6 . The second cover 6 is fixed on the first cover 2 and can also be used as a container for containing leaked liquid.
本申请实施例通过可旋转的悬臂式切割刀对放入实验容器中的组织进行切割，通过固定在第一盖上的多排齿和覆盖于其上的可旋转的结构件对放入实验容器中的组织进行碾磨，当悬臂式切割刀和结构件旋转时，实验容器中的组织反复地经受切割和碾磨处理，使得制备得到的生物样本中细胞分散更为充分。In the embodiment of the present application, the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon. When the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
实施例三Embodiment 3
图5是本申请实施例三提供的生物样本制备装置的结构示意图。结合图1和图5，生物样本制备装置包括外壳、电机和如实施例一或者实施例二描述的实验容器。电机设置于外壳内，实验容器放入外壳的缺口，容器本体1和第一盖2固定，电机的驱动轴与引导管31连接，驱动实验容器旋转以制备生物样本。FIG. 5 is a schematic structural diagram of the biological sample preparation device provided in the third embodiment of the present application. 1 and 5 , the biological sample preparation device includes a housing, a motor, and an experimental container as described in Embodiment 1 or Embodiment 2. The motor is arranged in the casing, the experimental container is placed in the notch of the casing, the container body 1 and the first cover 2 are fixed, and the drive shaft of the motor is connected with the guide tube 31 to drive the experimental container to rotate to prepare biological samples.
本申请实施例通过可旋转的悬臂式切割刀对放入实验容器中的组织进行切割，通过固定在第一盖上的多排齿和覆盖于其上的可旋转的结构件对放入实验容器中的组织进行碾磨，当悬臂式切割刀和结构件旋转时，实验容器中的组织反复地经受切割和碾磨处理，使得制备得到的生物样本中细胞分散更为充分。In the embodiment of the present application, the tissue placed in the experimental container is cut through a rotatable cantilever cutting knife, and the tissue placed in the experimental container is cut through the multiple rows of teeth fixed on the first cover and the rotatable structural members covered thereon. When the cantilever cutter and the structural member rotate, the tissue in the experimental container is repeatedly subjected to cutting and grinding treatment, so that the cells in the prepared biological sample are more fully dispersed.
尽管上面已经示出和描述了本申请的实施例，可以理解的是，上述实施例是示例性的，不能理解为对本申请的限制，本领域的普通技术人员在本申请的范围内可以对上述实施例进行变化、修改、替换和变型。Although the embodiments of the present application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limitations to the present application. Embodiments are subject to variations, modifications, substitutions and variations.

Claims (10)

  1. 一种实验容器，其特征在于，包括：An experimental container, characterized in that, comprising:
    具有开口端（11）和封闭端（12）的容器本体（1）；a container body (1) having an open end (11) and a closed end (12);
    连接到所述容器本体（1）开口端（11）的第一盖（2）；以及a first cover (2) connected to the open end (11) of the container body (1); and
    设置在所述第一盖（2）上的处理组件（3）；a processing assembly (3) arranged on the first cover (2);
    其中，所述处理组件（3）包括穿过所述第一盖（2）相对所述第一盖（2）可旋转的引导管（31）、固定在所述第一盖（2）上围绕所述引导管（31）布置的至少一排齿（32）和固定在所述引导管（31）上的搅拌元件（33）；Wherein, the processing assembly (3) comprises a guide tube (31) rotatable relative to the first cover (2) through the first cover (2), fixed on the first cover (2) to surround at least one row of teeth (32) arranged on the guide pipe (31) and a stirring element (33) fixed on the guide pipe (31);
    其中，所述搅拌元件（33）包括至少一个悬臂式切割刀（331）和与所述至少一排齿（32）相配合的结构件（332），所述结构件（332）从所述引导管（31）延伸并覆盖于所述至少一排齿（32）上。Wherein, the stirring element (33) comprises at least one cantilevered cutting blade (331) and a structural member (332) matched with the at least one row of teeth (32), the structural member (332) extending from the guide A tube (31) extends and covers the at least one row of teeth (32).
  2. 根据权利要求1所述的实验容器，其特征在于，对于同一横截面，所述至少一个悬臂式切割刀（331）朝向所述至少一排齿（32）的边与所述至少一排齿（32）的外周形成类三角形。The experimental container according to claim 1, characterized in that, for the same cross section, the edge of the at least one cantilever cutter (331) facing the at least one row of teeth (32) is the same as the at least one row of teeth (32). 32) to form a triangle-like outer periphery.
  3. 根据权利要求1所述的实验容器，其特征在于，所述至少一个悬臂式切割刀（331）的横截面为扇形。The experimental container according to claim 1, characterized in that, the cross section of the at least one cantilever cutting knife (331) is fan-shaped.
  4. 根据权利要求1所述的实验容器，其特征在于，所述结构件（332）还包括与所述至少一排齿（32）形成预设夹角的螺线形挡板（3321）。The experimental container according to claim 1, characterized in that, the structural member (332) further comprises a spiral baffle plate (3321) forming a preset angle with the at least one row of teeth (32).
  5. 根据权利要求4所述的实验容器，其特征在于，在所述螺线形挡板（3321）边缘开设至少一个泄压口（A）。The experimental container according to claim 4, characterized in that, at least one pressure relief port (A) is opened on the edge of the spiral baffle plate (3321).
  6. 根据权利要求1-5任一项所述的实验容器，其特征在于，所述结构件（332）和所述至少一个悬臂式切割刀（331）关于所述引导管（31）对称分布。The experimental container according to any one of claims 1-5, characterized in that, the structural member (332) and the at least one cantilevered cutting knife (331) are symmetrically distributed with respect to the guide tube (31).
  7. 根据权利要求1-5任一项所述的实验容器，其特征在于，所述引导管（31）和所述至少一排齿（32）通过密封件（4）连接。The experimental container according to any one of claims 1-5, characterized in that, the guide tube (31) and the at least one row of teeth (32) are connected by a seal (4).
  8. 根据权利要求1-5任一项所述的实验容器，其特征在于，所述实验容器还包括用于防泄漏的吸收环（5）和第二盖（6）。The experimental container according to any one of claims 1-5, characterized in that, the experimental container further comprises an absorption ring (5) for preventing leakage and a second cover (6).
  9. 根据权利要求1-5任一项所述的实验容器，其特征在于，所述引导管（31）还包括适于连接外部驱动轴的结合部。The experimental container according to any one of claims 1-5, characterized in that, the guide tube (31) further comprises a joint portion suitable for connecting to an external drive shaft.
  10. 一种生物样本制备装置，其特征在于，包括外壳、电机和如权利要求1-9任一项所述的实验容器，所述电机设置于所述外壳内，所述电机的驱动轴与所述引导管（31）连接，驱动所述实验容器旋转以制备生物样本。A biological sample preparation device, characterized in that it comprises a casing, a motor and the experimental container according to any one of claims 1-9, the motor is arranged in the casing, and the drive shaft of the motor is connected to the A guide tube (31) is connected to drive the experimental container to rotate to prepare a biological sample.
PCT/CN2021/114998 2021-04-29 2021-08-27 Experimental container and biological sample preparation device WO2022227354A1 (en)

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