WO2022141778A1 - 病毒自动化检测前处理*** - Google Patents

病毒自动化检测前处理*** Download PDF

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Publication number
WO2022141778A1
WO2022141778A1 PCT/CN2021/077499 CN2021077499W WO2022141778A1 WO 2022141778 A1 WO2022141778 A1 WO 2022141778A1 CN 2021077499 W CN2021077499 W CN 2021077499W WO 2022141778 A1 WO2022141778 A1 WO 2022141778A1
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unit
tube
sampling
turntable
cryogenic
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PCT/CN2021/077499
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English (en)
French (fr)
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吴晨龙
王安敏
李小娟
刘家朋
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倍仪昇智能科技(苏州)有限公司
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Publication of WO2022141778A1 publication Critical patent/WO2022141778A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00435—Refrigerated reagent storage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00445—Other cooling arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0418—Plate elements with several rows of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0429—Sample carriers adapted for special purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0429—Sample carriers adapted for special purposes
    • G01N2035/0434—Sample carriers adapted for special purposes in the form of a syringe or pipette tip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0429—Sample carriers adapted for special purposes
    • G01N2035/0436—Sample carriers adapted for special purposes with pre-packaged reagents, i.e. test-packs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0496—Other details
    • G01N2035/0498—Drawers used as storage or dispensing means for vessels or cuvettes

Definitions

  • the invention relates to the technical field of automation equipment, in particular to a preprocessing system for automatic virus detection.
  • Virus detection includes multiple processes, from virus sampling to culture amplification detection, which are all implemented manually, which is not only inefficient, but also cannot be effectively monitored during the detection process, and the detection results vary from person to person. .
  • the virus poses a safety hazard to the human body. Although it is carried out in a biological safety cabinet, safety accidents cannot be avoided.
  • the detection operation process including liquid sampling, container opening and closing, precise liquid injection, etc., also involves the operation control of each equipment, which not only tests the operator's work intensity, but also tests the operator's patience.
  • the purpose of the present invention is to solve the above problems and provide a pretreatment system for automatic virus detection, which can complete the pretreatment processes such as mixing, liquid separation, subpackaging, and inactivation after sampling from the virus through the rational design of automated equipment.
  • a pretreatment system for automated virus detection characterized in that it includes a sample input unit, a sampling unit, a turntable unit, a cryopreservation tube unit, a metal bath unit, a pretreatment output unit and a robotic arm unit, and the sample input unit is used to
  • the sampling tube is arranged, the cryogenic tube unit sends the cryogenic tube to the turntable unit through the robotic arm unit, and after the sampling unit extracts the reagent from the mentioned sampling tube, the reagent is divided into three cryogenic tubes through the turntable unit.
  • the robotic arm unit sends one cryovial to the metal bath unit, and two cryovials are sent back to the cryogenic tube unit, and the metal bath unit inactivates the reagents in the cryovials and sends them to the pre-processing output unit, which is output to the subsequent system construction system, and the subpackaged cryovials of the cryopreservation tube unit are sent to cryopreservation equipment.
  • the sample input unit also includes a vibration module, a sample rack is provided on the vibration module, a sampling tube is arranged on the sample rack, a reset mechanism is provided on the vibration module, and the reset mechanism makes the sample rack vibrate to complete Then return to the original position.
  • the robotic arm unit is provided with a gripping device, a liquid taking device, and a lid opening and closing device, and the gripping device carries out a transport operation for the containers of each unit.
  • the sampling unit is located between the sample input unit and the turntable unit, the sampling unit includes a sampling tube fixing part, and the gripping device of the robotic arm unit sends the sampling tube on the sample holder to the sample fixing part for fixing.
  • the cap opening and closing device opens the cap of the sampling tube
  • the liquid sampling device absorbs the quantitative test solution from the sampling tube
  • the cap opening and closing device closes the cap of the sampling tube, and is returned to the original position of the sample holder by the clamping device.
  • each station is provided with a freezing tube clamping tool, and one side of the turntable unit is also provided with a freezing tube opening and closing cover part, and the liquid sampling device will take samples.
  • the test solution in the tube is sent to the cryogenic tube on the turntable unit.
  • the cryogenic tube unit includes a cryogenic tube array
  • the gripping device of the robotic arm unit takes out three cryogenic tubes from the cryogenic tube array and sends them to the turntable unit
  • the liquid taking device takes the test solution in the sampling tube.
  • the liquid is dispensed into three cryovials, and the gripping device returns two cryovials to the cryovial array and one cryotube to the metal bath unit.
  • the cryogenic tube unit further includes a rotary transfer rack, and the rotary transfer rack sends three cryogenic tubes to the turntable unit at a time, and after the cryogenic tubes are filled with liquid, they are returned by the rotary transfer rack.
  • the metal bath unit is located between the turntable unit and the freezing tube unit, and the metal bath unit heats and inactivates one of the freezing tubes.
  • the pre-processing output unit includes an orifice plate and an output rotating arm, the orifice plate is arranged on the output rotating arm, and the liquid taking device separates the inactivated cryo-tubes into the orifice plate.
  • a suction head unit is further included between the sample input unit and the turntable unit, and the suction head unit includes a suction head box and a suction tip discarding box. tip, and when finished dispensing, discard the tip in the tip disposal box.
  • FIG. 4 is a partial enlarged view of the suction head unit and the pretreatment output unit
  • Figure 6 is a partial enlarged view of the cryovial unit and the metal bath unit.
  • Rotary conveyor 18. Orifice plate;
  • the virus automated detection pre-processing system is installed in a biological safety cabinet, standardized in strict accordance with the national virus safety detection requirements, and necessary parameter optimization is performed for different types of virus samples to meet the needs of virus detection.
  • the virus detection pretreatment system is arranged in an L-shaped arrangement, including a sample input unit 1, a sampling unit 2, a tip unit 3, a turntable unit 4, a cryogenic tube unit 5, a metal bath unit 6, a pretreatment output unit 7 and a robotic arm unit 8.
  • Sample input unit 1, sampling unit 2, tip unit 3, turntable unit 4, metal bath unit 6, pretreatment output unit 7 are located in one row, cryogenic tube unit 5 is located on one side of the L-shape, robotic arm unit 8
  • the rails are set in multiple positions above the system, and are reasonably arranged according to the needs of the movement.
  • the sample input unit 1 is used for arranging the sampling tubes 9, the cryogenic tube unit 5 sends the cryogenic tubes to the turntable unit 4 through the robotic arm unit 8, the sampling unit 2 extracts the reagents from the mentioned sampling tubes 9, and passes through the turntable unit 4.
  • the reagents are divided into three cryovials, the robotic arm unit 8 sends one cryovial to the metal bath unit 6, and the two cryotubes are returned to the cryogenic tube unit 5, and the metal bath unit 6 sends the After the reagent is inactivated, it is sent to the pre-processing output unit 7, and is output to the subsequent system construction system, and the subpackaged cryovials of the cryopreservation tube unit 5 are sent to the cryopreservation equipment.
  • the mechanical arm unit 8 includes a beam in the XYZ direction arranged on the side of each working unit.
  • Each beam is provided with a clamping device 10, a liquid sampling device 11, a lid opening and closing device 12 and other functional mechanical arms to achieve clamping movement, extraction and The functions of injecting quantitative solution, clamping and rotating, etc., realize the handling, opening and closing of the container such as sampling tube 9 and cryopreservation tube, as well as the action of taking and injecting liquid, and realizing the orderly development of the pre-treatment work for virus detection.
  • the sample input unit 1 also includes a vibration module, a sample rack 13 is provided on the vibration module, a sampling tube 9 is arranged on the sample rack 13, a reset mechanism is provided on the vibration module, and the reset mechanism makes the sample rack 13 return to the initial stage after the vibration is completed Location.
  • the vibration module is realized by the motor-driven eccentric link, and the reset is realized by the motor encoder or the tapered hole positioning method.
  • Two rows of sample racks 13 are arranged within the controllable range of the robotic arm, and can be positioned by a positioning mechanism, so that the robotic arm can be precisely positioned and manipulated.
  • the sampling unit 2 is located between the sample input unit 1 and the turntable unit 4, the sampling unit 2 includes a sampling tube 9 fixing part, and the gripping device of the robotic arm unit 8 sends the sampling tube 9 on the sample rack 13 to the sample
  • the cap opening and closing device 12 opens the cap of the sampling tube 9
  • the liquid taking device 11 absorbs the quantitative test solution from the sampling tube 9
  • the cap opening and closing device 12 closes the cap of the sampling tube 9, and is sent by the clamping device.
  • the fixed part of the sampling tube 9 defines the sampling tube 9 in a fixed position through the elastic clip and the positioning plate, so as to facilitate the direct operation of the robotic arm unit 8 .
  • the tip unit 3 is located between the sample input unit 1 and the turntable unit 4.
  • the tip unit 3 includes a tip box 14 and a tip discard box 15.
  • the liquid taking device 11 takes liquid from the tip box 14
  • the tips are pressed into the tip box 14 through the liquid taking device 11 on the robotic arm, and when discarded, the tips are directly placed in the tip discard box 15. Can be removed from the top.
  • a plurality of cryopreservation tube stations are arranged on the turntable unit 4, and a cryopreservation tube clamping tool 16 is arranged on each station, and a cryopreservation tube opening and closing cover part is also provided on one side of the turntable unit 4, and a liquid sampling device is provided.
  • 11 Send the test solution in the sampling tube 9 to the freezing tube on the turntable unit 4.
  • the freezing tube clamping tool 16 on each station is a double-spring device, a clamping block is arranged between the two springs, and the freezing tube is placed between the clamping blocks.
  • cryopreservation tube opening and closing cover part 20 completes the opening and closing of the cryopreservation tube.
  • the cryotube unit 5 includes a cryotube array, and one side is a part of a robotic arm unit 8 for grabbing cryotubes from the cryotube array to the turntable unit 4.
  • the general process is a robotic arm unit.
  • the clipping device in 8 takes out three cryovials from the cryotube array and sends them to the turntable unit 4.
  • the liquid taking device 11 divides the test solution in the sampling tube 9 into the three cryotubes, and the clipping device freezes two tubes. The tubes are sent back to the cryovial array and one cryovial is sent to the metal bath unit 6 .
  • the cryogenic tube unit 5 further includes a rotary transfer rack 17 , which sends three cryogenic tubes to the turntable unit 4 at a time. After the cryogenic tubes are filled with liquid, they are returned by the rotary transfer rack 17 . By rotating the transfer rack 17, the transition from the cryogenic tube on one side to the turntable unit 4 is realized, the size of the equipment is greatly reduced, and the miniaturization and compactness of the equipment is realized.
  • the metal bath unit 6 is located between the turntable unit 4 and the cryogenic tube unit 5, and the metal bath unit 6 heats and inactivates one of the cryogenic tubes.
  • the metal bath unit 6 is inactivated at a constant temperature by electric heating and temperature control components, and the temperature is maintained at 56°C.
  • the pretreatment output unit 7 includes an orifice plate 18 and an output rotating arm 19, the orifice plate 18 is arranged on the output rotating arm 19, and the liquid taking device 11 dispenses the inactivated cryotubes on the orifice plate 18 Inside.
  • the orifice plates 18 are generally two standard 96-well orifice plates 18 arranged side by side.
  • the output rotating arm 19 rotates to output, and the orifice plates 18 are extended from the biological safety cabinet, so as to facilitate the subsequent removal operation by the robot.
  • the inactivated cryopreservation tube is also transported through the turntable unit 4 , and then subjected to liquid manipulation through the liquid sampling device 11 of the robotic arm unit 8 .

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Robotics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

一种病毒自动化检测前处理***，包括样品输入单元（1）、取样单元（2）、转盘单元（4）、冻存管单元（5）、金属浴单元（6）、前处理输出单元（7）和机械臂单元（8）。样品输入单元（1）用于将取样管（9）布置，冻存管单元（5）通过机械臂单元（8）将冻存管送至转盘单元（4），取样单元（2）从取样管（9）中提取试剂后，通过转盘单元（4）将试剂分装在三个冻存管中，机械臂单元（8）将一个冻存管送至金属浴单元（6），两个冻存管送回冻存管单元（5），金属浴单元（6）将冻存管内的试剂灭活后，送至前处理输出单元（7），输出至后续体系构建***，冻存管单元（5）的分装冻存管送至冷冻保存设备。该***可完成从病毒采样后的混合、分液、分装、灭活等前处理步骤。

Description

病毒自动化检测前处理*** 技术领域
本发明涉及自动化设备技术领域，尤其涉及一种病毒自动化检测前处理***。
背景技术
病毒自动化检测过程替代手工检测是大势所趋，病毒检测包括多个流程，从病毒采样到培养扩增检测，都是通过人工实现，不仅效率低下，而且检测过程无法进行有效监控，检测结果因人而异。另外病毒对人体产生安全隐患，虽然在生物安全柜中进行，但还是不能避免产生安全事故。
检测的操作过程，包括液体的取样、容器的开合盖、精确的注液等操作，还涉及各个设备的操作控制，不仅考验操作人员的工作强度，还对操作人员的耐心也是一个考验。
而将操作人员从枯燥的重复劳动中解放出来，并提高检测精度以及检测数据的可溯性，检测自动化势在必行。
发明内容
本发明的目的是为了解决上述问题，提供一种病毒自动化检测前处理***，通过自动化设备的合理设计，完成从病毒采样后的混合、分液、分装、灭活等前处理工艺。
本发明采取的技术方案是：
一种病毒自动化检测前处理***，其特征是，包括样品输入单元、取样单元、转盘单元、冻存管单元、金属浴单元、前处理输出单元和机械臂单元，所 述样品输入单元用于将取样管布置，所述冻存管单元通过机械臂单元将冻存管送至转盘单元，所述取样单元从提述取样管中提取试剂后，通过转盘单元将试剂分装在三个冻存管中，所述机械臂单元将一个冻存管送至金属浴单元，两个冻存管送回冻存管单元，所述金属浴单元将冻存管内的试剂灭活后，送至前处理输出单元，输出至后续体系构建***，所述冻存管单元的分装冻存管送至冷冻保存设备。
进一步，所述样品输入单元还包括振动模块，所述振动模块上设置样品架，所述样品架上布置取样管，所述振动模块上设置复位机构，所述复位机构使所述样品架振动完成后回复到初始位置。
进一步，所述机械臂单元上设置夹取装置，取液装置，开合盖装置，所述夹取装置将各单元的容器进行搬运操作。
进一步，所述取样单元位于样品输入单元与转盘单元之间，所述取样单元包括取样管固定部件，所述机械臂单元的夹取装置将样品架上的取样管送至样品固定部件上固定后，开合盖装置对取样管开盖，取液装置从取样管内吸取定量试液，开合盖装置再将取样管合盖，由夹取装置送回样品架的原位置。
进一步，所述转盘单元上设置多个冻存管工位，每个工位上设置冻存管夹紧工装，转盘单元一侧还设置冻存管开合盖部件，所述取液装置将取样管内的试液送至转盘单元上的冻存管。
进一步，所述冻存管单元包括冻存管阵列，所述机械臂单元的夹取装置从冻存管阵列中取出三根冻存管送至转盘单元，所述取液装置将取样管内的试液分液至三根冻存管，夹取装置将两根冻存管送回至冻存管阵列，一根冻存管送至金属浴单元。
进一步，所述冻存管单元还包括旋转传送架，旋转传送架一次将三个冻存管送至转盘单元，冻存管完成注液后，由旋转传送架送回。
进一步，所述金属浴单元位于转盘单元和冻存管单元之间，所述金属浴单元对其中一根冻存管加热灭活。
进一步，所述前处理输出单元包括孔板和输出转臂，所述孔板设置在输出转臂上，所述取液装置将灭活后的冻存管分装于孔板内。
进一步，在所述样品输入单元与转盘单元之间还包括吸头单元，所述吸头单元包括吸头盒和吸头丢弃盒，所述取液装置取液时从吸头盒中装上吸头，完成放液后，将吸头弃于吸头丢弃盒。
本发明的有益效果是：
(1)自动化实现试样的振荡混合，分液分装，灭活分装等工艺过程；
(2)通过合理布局，使用机械臂单元完成各项前处理操作；
(3)数据可追溯，生成的孔板样品一致性好，为后续病毒检测精度提供有力保障。
附图说明
附图1是本发明的立体结构示意图(除去生物安全柜后)；
附图2是本发明的样品输入单元的局部放大图；
附图3是取样单元的局部放大图；
附图4是吸头单元和前处理输出单元的局部放大图；
附图5是转盘单元的局部放大图；
附图6是冻存管单元和金属浴单元的局部放大图。
附图中的标号分别为：
1.样品输入单元；          2.取样单元；
3.吸头单元；              4.转盘单元；
5.冻存管单元；            6.金属浴单元；
7.前处理输出单元；        8.机械臂单元；
9.取样管；                10.夹紧装置；
11.取液装置；             12.开合盖装置；
13.样品架；               14.吸头盒；
15.吸头丢弃盒；           16.冻存管夹紧工装；
17.旋转传送架；           18.孔板；
19.输出转臂；             20.冻存管开合盖部件。
具体实施方式
下面结合附图对本发明病毒自动化检测前处理***的具体实施方式作详细说明。
参见附图1,病毒自动化检测前处理***安装在生物安全柜中，严格按照国家病毒安全检测要求进行规范化，对于不同类别的病毒样品进行必要的参数优化以满足病毒检测需要。
病毒检测前处理***设置成L形布置，包括样品输入单元1、取样单元2、吸头单元3、转盘单元4、冻存管单元5、金属浴单元6、前处理输出单元7和机械臂单元8，样品输入单元1、取样单元2、吸头单元3、转盘单元4、金属浴单元6、前处理输出单元7位于一排，冻存管单元5位于L形的一侧，机械臂单元8通过轨道设置在***的上方多个位置，根据移动需要进行合理布置。
样品输入单元1用于将取样管9布置，冻存管单元5通过机械臂单元8将冻存管送至转盘单元4，取样单元2从提述取样管9中提取试剂后，通过转盘单元4将试剂分装在三个冻存管中，机械臂单元8将一个冻存管送至金属浴单元6，两个冻存管送回冻存管单元5，金属浴单元6将冻存管内的试剂灭活后，送至前处理输出单元7，输出至后续体系构建***，冻存管单元5的分装冻存管送至冷冻保存设备。
机械臂单元8包括设置于各工作单元侧边的XYZ方向的横梁，各梁上分别设置夹紧装置10、取液装置11，开合盖装置12等功能机械臂，实现夹持移动，抽取和注入定量溶液、夹持旋转等功能，实现对取样管9、冻存管等容器的搬运、开合盖和取液注液动作，实现病毒检测前处理工作的有序开展。
参见附图2,样品输入单元1还包括振动模块，振动模块上设置样品架13，样品架13上布置取样管9，振动模块上设置复位机构，复位机构使样品架13振动完成后回复到初始位置。振动模块通过电机驱动偏心连杆实现，通过电机编码器或锥形孔定位方式实现复位。样品架13设置两排，在机械臂的可控范围内布置，可通过定位机构实现定位，以使机械臂能够精确地定位的操控。
参见附图3,取样单元2位于样品输入单元1与转盘单元4之间，取样单元2包括取样管9固定部件，机械臂单元8的夹取装置将样品架13上的取样管9送至样品固定部件上固定后，开合盖装置12对取样管9开盖，取液装置11从取样管9内吸取定量试液，开合盖装置12再将取样管9合盖，由夹取装置送回样品架13的原位置。取样管9固定部件通过弹性夹和定位板将取样管9限定在固定位置，方便机械臂单元8的直接操作。
参见附图4，吸头单元3位于样品输入单元1与转盘单元4之间，吸头单 元3包括吸头盒14和吸头丢弃盒15，取液装置11取液时从吸头盒14中装上吸头，完成放液后，将吸头弃于吸头丢弃盒15。在不同种试液的液体处理过程中，有流程中有必要的要求下，要进行吸头的更换。比如更换液体种类，不同阶段试剂更换时，一定要进行吸头的更换，更换过程通过机械臂上的取液装置11从吸头盒14上压入吸头，丢弃时直接在吸头丢弃盒15上方褪去即可。
参见附图5，转盘单元4上设置多个冻存管工位，每个工位上设置冻存管夹紧工装16，转盘单元4一侧还设置冻存管开合盖部件，取液装置11将取样管9内的试液送至转盘单元4上的冻存管。每个工位上的冻存管夹紧工装16均为双弹簧装置，两个弹簧之间设置夹块，冻存管放置于夹块之间，通过对夹块的施力，使夹块夹紧冻存管，再通过冻存管夹紧工装16将冻存管夹紧后，冻存管开合盖部件20完成对冻存管的开合盖。
参见附图6,冻存管单元5包括冻存管阵列，一侧为机械臂单元8的一部分，用于从冻存管阵列中抓取冻存管至转盘单元4，一般过程是机械臂单元8的夹取装置从冻存管阵列中取出三根冻存管送至转盘单元4，取液装置11将取样管9内的试液分液至三根冻存管，夹取装置将两根冻存管送回至冻存管阵列，一根冻存管送至金属浴单元6。
在冻存管单元5还包括旋转传送架17，旋转传送架17一次将三个冻存管送至转盘单元4，冻存管完成注液后，由旋转传送架17送回。通过旋转传送架17，实现一侧的冻存管转移至转盘单元4的过渡，大大缩小了设备的尺寸，实现设备的小型化紧凑性。
金属浴单元6位于转盘单元4和冻存管单元5之间，金属浴单元6对其中一根冻存管加热灭活。金属浴单元6通过电加热和温控部件实现恒温灭活，温 度维持在56℃。
继续参见附图4,前处理输出单元7包括孔板18和输出转臂19，孔板18设置在输出转臂19上，取液装置11将灭活后的冻存管分装于孔板18内。孔板18一般为标准96孔孔板18两个并排布置，完成后输出转臂19转动输出，将孔板18从生物安全柜中伸出，便于后续机器人取出操作。灭活后的冻存管也是通过转盘单元4进行传送，再通过机械臂单元8的取液装置11进行液体操作。
以上仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (10)

  1. 一种病毒自动化检测前处理***，其特征在于：包括样品输入单元、取样单元、转盘单元、冻存管单元、金属浴单元、前处理输出单元和机械臂单元，所述样品输入单元用于将取样管布置，所述冻存管单元通过机械臂单元将冻存管送至转盘单元，所述取样单元从提述取样管中提取试剂后，通过转盘单元将试剂分装在三个冻存管中，所述机械臂单元将一个冻存管送至金属浴单元，两个冻存管送回冻存管单元，所述金属浴单元将冻存管内的试剂灭活后，送至前处理输出单元，输出至后续体系构建***，所述冻存管单元的分装冻存管送至冷冻保存设备。
  2. 根据权利要求1所述的病毒自动化检测前处理***，其特征在于：所述样品输入单元还包括振动模块，所述振动模块上设置样品架，所述样品架上布置取样管，所述振动模块上设置复位机构，所述复位机构使所述样品架振动完成后回复到初始位置。
  3. 根据权利要求1所述的病毒自动化检测前处理***，其特征在于：所述机械臂单元上设置夹取装置，取液装置，开合盖装置，所述夹取装置将各单元的容器进行搬运操作。
  4. 根据权利要求2所述的病毒自动化检测前处理***，其特征在于：所述取样单元位于样品输入单元与转盘单元之间，所述取样单元包括取样管固定部件，所述机械臂单元的夹取装置将样品架上的取样管送至样品固定部件上固定后，开合盖装置对取样管开盖，取液装置从取样管内吸取定量试液，开合盖装置再将取样管合盖，由夹取装置送回样品架的原位置。
  5. 根据权利要求4所述的病毒自动化检测前处理***，其特征在于：所述转盘单元上设置多个冻存管工位，每个工位上设置冻存管夹紧工装，转盘单元 一侧还设置冻存管开合盖部件，所述取液装置将取样管内的试液送至转盘单元上的冻存管。
  6. 根据权利要求5所述的病毒自动化检测前处理***，其特征在于：所述冻存管单元包括冻存管阵列，所述机械臂单元的夹取装置从冻存管阵列中取出三根冻存管送至转盘单元，所述取液装置将取样管内的试液分液至三根冻存管，夹取装置将两根冻存管送回至冻存管阵列，一根冻存管送至金属浴单元。
  7. 根据权利要求6所述的病毒自动化检测前处理***，其特征在于：所述冻存管单元还包括旋转传送架，旋转传送架一次将三个冻存管送至转盘单元，冻存管完成注液后，由旋转传送架送回。
  8. 根据权利要求6所述的病毒自动化检测前处理***，其特征在于：所述金属浴单元位于转盘单元和冻存管单元之间，所述金属浴单元对其中一根冻存管加热灭活。
  9. 根据权利要求6所述的病毒自动化检测前处理***，其特征在于：所述前处理输出单元包括孔板和输出转臂，所述孔板设置在输出转臂上，所述取液装置将灭活后的冻存管分装于孔板内。
  10. 根据权利要求3所述的病毒自动化检测前处理***，其特征在于：在所述样品输入单元与转盘单元之间还包括吸头单元，所述吸头单元包括吸头盒和吸头丢弃盒，所述取液装置取液时从吸头盒中装上吸头，完成放液后，将吸头弃于吸头丢弃盒。
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