WO2022122628A2 - Device for optical imaging of arthropods - Google Patents

Device for optical imaging of arthropods Download PDF

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Publication number
WO2022122628A2
WO2022122628A2 PCT/EP2021/084336 EP2021084336W WO2022122628A2 WO 2022122628 A2 WO2022122628 A2 WO 2022122628A2 EP 2021084336 W EP2021084336 W EP 2021084336W WO 2022122628 A2 WO2022122628 A2 WO 2022122628A2
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WO
WIPO (PCT)
Prior art keywords
multicellular
accommodation
platform
camera
module
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Application number
PCT/EP2021/084336
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French (fr)
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WO2022122628A3 (en
Inventor
Samir KASSI
Florent SEBBANE
Original Assignee
(Inserm) Institut National De La Santé Et De La Recherche Médicale
Centre National De La Recherche Scientifique
Université de Lille
Institut Pasteur De Lille
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Publication of WO2022122628A2 publication Critical patent/WO2022122628A2/en
Publication of WO2022122628A3 publication Critical patent/WO2022122628A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • TITLE Device for optical imaging of arthropods.
  • This invention relates to the field of monitoring arthropods.
  • the present invention provides a device for optical imaging of at least one arthropod, the at least one arthropod being accommodated in an accommodation cell of a multi-cellular accommodation module, the accommodation cell being provided with a first opening on an observable side of the multi-cellular accommodation module, the device comprising:
  • a camera configured to image the whole, or a portion, of the observable side of the multicellular accommodation module, said portion comprising the first opening of the accommodation cell, an image of the at least one arthropod being formed through the first opening;
  • a position of the camera with respect to the observable side of the multicellular accommodation module being adjustable so that a distance between an objective of the camera and a central point of the portion of the multicellular accommodation module may be set to any value between a minimum value and a maximum value.
  • the invention comprises one or more of the following features, alone or in any combination technically compatible.
  • the minimum value is comprised between 0 cm and 5 cm, in particular between 0 cm and 1 cm, more particularly between 0 mm and 1 mm.
  • the distance between the objective of the camera can be chosen very small and the device is compact and nevertheless configured to provide high resolution images of the at least one arthropod, especially with high magnification objectives, typically with magnifications comprised between x0,1 and x10, in particular with magnifications comprised between x1 and x5, more particularly with a magnification equal to x4.
  • the device comprises an illumination source configured for illuminating the at least one arthropod through the first opening when the illumination source is switched on;
  • the accommodation cell is provided with a second opening on a second side, opposite to the observable side, of the multi-cellular accommodation module, and the illumination source is configured for illuminating the at least one arthropod through the second opening when the illumination source is switched on, so that the image formed through the first opening is a transmission image.
  • the device comprises a transparent support, the transparent support being movable with respect to the camera, and wherein the observable side of the multicellular accommodation module is lying on and removably attached to the transparent support, the portion of the multicellular accommodation module being imaged by the camera through the transparent support, said portion being determined by a position the movable transparent support.
  • the objective of the camera is configured to image the whole or any portion of the observable side of the multicellular accommodation module, depending on the position of the movable transparent support, each portion comprising different accommodation cells, each accommodation cell accommodating an individual arthropod.
  • the transparent support is movable according to a first direction, said first direction being parallel to the observable side of the multicellular accommodation module.
  • the transparent support is movable according to a second direction, said second direction being parallel to the observable side of the multicellular accommodation module.
  • the transparent support is a bottom of a basket configured to house the multicellular accommodation module
  • the device further comprising a first driving module on a first platform, the basket being movably attached to the first platform, the basket having a basket opening opposite to the bottom of the basket on one side of the first platform, the first driving module being configured to drive the translation of the basket along a first direction, the first platform comprising a first platform opening, the device further comprising a second driving module on a second platform, the second driving module being configured to drive the translation of the first platform along a second direction, the second platform comprising a second platform opening, the first platform opening and the second platform opening being superposed, wherein the basket is configured to pass through the first platform opening and the second platform opening so that the bottom of the basket, and the camera are on the same other side of the second platform.
  • the camera, and the camera objective may be as close as possible to the observable side of the multicellular accommodation module when the observable side of the multicellular accommodation module is lying on the bottom of the basket.
  • the first driving module comprises at least one motor configured to move the basket and the transparent support at the bottom of the basket, relative to the first platform, according to a translation along the first direction, from a first position of the basket to a second position of the basket, a first extension of the translation being defined by a maximum distance between the first position and the second position of the basket, wherein the first extension of the translation is determined by a dimension of the first opening along the first direction
  • the second driving module comprises a second motor to move the first platform, and the basket movably attached to the first platform, along the second direction, relative to the second platform, third position of the basket and a fourth position of the basket, a second extension of the translation being defined by a maximum distance between the third position and the fourth position of the basket, wherein the second extension of the translation is determined by a dimension of the second opening along the second direction.
  • the extension of the displacement in any direction can be determined by the dimensions of the first opening and second opening, which can be adapted to the dimensions of the multicellular accommodation module.
  • the position of the transparent support along the first or the second direction is controlled with a precision less 2 pm, the precision being preferably less than 1 pm.
  • the position of the multicellular accommodation module along the first or the second direction is controlled with the same precision, i.e. a precision less 2 pm, the precision being preferably less than 1 pm.
  • a first extension of the multicellular accommodation module along the first direction is comprised between 0 and 200 mm, preferably between 0 and 100 mm, more preferably between 0 and 50 mm.
  • a second extension of the multicellular accommodation module along the second direction is comprised between 0 and 200 mm, preferably between 0 and 100 mm, more preferably between 0 and 50 mm.
  • a first extension of the movement of the multicellular accommodation module along the first direction is equal to the first extension of the multicellular accommodation module along the first direction.
  • a second extension of the movement of the multicellular accommodation module along the second direction is equal to the second extension of the multicellular accommodation module along the second direction.
  • the portion of the multicellular accommodation module being imaged by the camera can be determined according to the precision of the position of the movable transparent support.
  • the camera of the device can image any portion of the multicellular accommodation module.
  • the camera of the device can image any portion of a specific multicellular accommodation module having extensions in the first and in the second directions corresponding to a standard format comprising, for example 96 or 144 cells in both direction.
  • the number of cells of the multicellular accommodation module may be any number, provided the number of cells multiplied by the dimension of each cell respectively along the first or along the second direction fits with the extension of the multicellular accommodation module respectively along the first direction or along the second direction.
  • the camera comprises a matrix of detectors, each detector of the matrix having a detector first extension in the first direction, and a detector second extension in the second direction.
  • the detector first extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
  • the detector second extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
  • the position of the multicellular accommodation module in the first direction and/or in the second direction is controlled with a precision which is better than a camera resolution respectively in the first and the second directions corresponding respectively to the first detector extension and/or to the second detector extension.
  • the at least one arthropod is bioluminescent
  • the camera is further configured to image the portion of the multicellular accommodation module with only bioluminescent illumination from the arthropod, thus providing a bioluminescent image of the portion of the multicellular accommodation module.
  • the small distance between the objective of the camera and the central point of the portion of the multicellular accommodation module is appropriate to optimise light collected, which is particularly adapted to bioluminescent imaging considering that the amount of light emitted by the sample can be very low.
  • the bioluminescent image of the portion of the multicellular accommodation module may be superposed onto the transmission image of the portion of the multicellular accommodation module obtained with an illumination source switched on, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
  • the device further comprises :
  • a first filter-holder carousel configured to place a spectral filter between an illumination source and the portion of the multicellular accommodation module, when the illumination source is switched on;
  • a second filter-holder carousel configured to place a spectral filter between the portion of the multicellular accommodation module and the camera, when the illumination source is switched off, and when the at least one arthropod is bioluminescent.
  • multispectral versions of the transmission/bioluminescent images of the portion of the multicellular accommodation module may be formed on the camera and may be superposed onto one another, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
  • the invention provides a system for optical imaging of at least one arthropod, the system comprising a device according to anyone of the embodiments dexcribed hereinabove, and the multi-cellular accommodation module.
  • FIG. 1 is an exploded perspective view of the device according to the invention.
  • the device 1 is configured to embed a multi-cellular accommodation module 9, comprising at least one accommodation cell, preferably a plurality of accommodation cells, each of the accommodation cells being configured to host an arthropod; the accommodation cells are provided with a first opening on an observable side of the multi-cellular accommodation module 9 : for example, on the figure the observable side of the multi-cellular accommodation module 9 is the downward side, lying on a transparent support 8.
  • the device 1 comprises a camera, not represented on the figure, configured to observe the observable side of the multi-cellular accommodation module 9 through the transparent support 8.
  • the camera is attached to the device 1 and configured to observe from below the observable side of the multi-cellular accommodation module 9, in the direction D of observation towards the observable side of the multi-cellular accommodation module 9.
  • the camera is further configured to image the whole, or a portion, of the observable side of the multicellular accommodation module 9.
  • the camera may be attached to the device 1 and the transparent support 8 is configured to be movable with respect to the camera, so that when the transparent support 8 is displaced with respect to the attached camera, the imaged portion of the observable side of the multicellular accommodation module 9 is displaced accordingly.
  • the camera may be attached to the device 1 so that its position with respect to the observable side of the multicellular accommodation module 9 is adjustable in a direction of observation D; thus, a distance between an objective of the camera and a central point of the observed portion of the multicellular accommodation module 9 may be set to any value between a minimum value and a maximum value.
  • the minimum value is comprised between 0 cm and 5 cm, in particular between 0 cm and 1 cm, more particularly between 0 mm and 1mm; according to these provisions, the distance between the objective of the camera can be chosen very small and the device is compact and nevertheless configured to provide high resolution images of the at least one arthropod hosted in the cell of the observed portion of the multicellular accommodation module 9, especially with high magnification objectives; typically magnifications may be comprised between x0,1 and x10, in particular comprised between x1 and x5, more particularly magnification is equal to x4.
  • the transparent support 8 may be the bottom of a basket 8, the basket 8 being attached to a frame 2, said frame 2 being movably attached to at least one longitudinal rail 7, so that the movement of the frame 2 may is guided along the at least one longitudinal rail 7.
  • a motor 4 driving a belt 6, via a pulley system 5, may be configured to drive the translation of the frame 2 in a first direction YY’, along the at least one longitudinal rail 7; according to these provisions, a position of the frame 2, consequently a position of the observed portion of the multicellular accommodation module 9 relative to the camera may be determined precisely along the at least one longitudinal rail 7.
  • the position of the observed portion of the observable side of the multicellular accommodation module 9 may be controlled in the first direction YY’ with a precision less 2 pm, the precision being more particularly less than 1 pm.
  • the first driving module comprising a first platform 3, the motor 4, the at least one driving belt 6 and the at least one longitudinal rail 7, the motor 4, and the at least one longitudinal rail 7 being both attached on the first platform 3, the first driving module being thus configured to drive, via the pulley system 5 and the driving belt 6, the translation movement of the frame 2 and, consequently of the observable portion of the multicellular accommodation module 9, along a first direction, with respect to the camera which is configured to be fixed along the first direction.
  • the first driving module is itself translated by a second driving module, along a second direction XX’, transversal to the first direction YY’, the second driving module comprising a second platform 10, with a second motor 4’, a second belt 6’, and a second pulley system 5’, and at least one other longitudinal rail 7’, the second motor 4’ being configured to drive the second belt 6’, via the second pulley system 5’, along the at least one other longitudinal rail 7’; thus a position of the observed portion of the multicellular accommodation module 9 relative to the camera may be determined precisely along the second direction XX’.
  • the first platform 3, with the first driving module is movable along the second direction XX’, relative to the second platform 10, which is fixed, the camera being fixed with respect to the second platform 10, in the first and in the second direction YY’ and XX’; as indicated hereinabove, the position of the camera in the direction of observation D is adjustable so that near-field imaging of the observable portion of the multicellular accommodation module 9 may be performed, at any position of said portion along the first and/or along the second direction YY’ and XX’.
  • a position of the observed portion of the observable side of the multicellular accommodation module 9 may be controlled in the first direction YY’ and/or in the second direction XX’ with a precision less 2 pm, the precision being more particularly less than 1 pm.
  • the first platform 3 of the first driving module comprises a first platform 3 opening
  • the second platform 10 of the second driving module comprises a second platform 10 opening
  • the first platform 3 opening and the second platform 10 opening are superposed
  • the basket 8, attached to the frame 2 which is movably attached on one side of the first platform 3 is configured to pass through the first platform 3 opening and the second platform 10 opening so that the multicellular accommodation module 9 lying on the bottom of the basket 8 and the camera are on the same other side of the second platform 10.
  • the camera more particularly the camera objective may be as close as possible to the observable side of the multicellular accommodation module 9.
  • the basket 8, the bottom of which is transparent and configured to support the multicellular accommodation module 9, may have any required dimensions, in particular to lodge multicellular accommodation modules 9 which may comprise 96 or 144 cells in the first YY’ and/or in the second XX’ directions. Accordingly, an extension of the multicellular accommodation module, consequently of the basket 8, along the first direction YY’, or in the second direction XX’, may be comprised between 0 and 200 mm, in particular between 0 and 100 mm, more particularly between 0 and 50 mm, depending on the number of cells and on the dimension of each cell respectively along the first or along the second direction.
  • the device 1 may in particular comprise an illumination source, not represented on figure 1 , the illumination source being configured for illuminating the at least one arthropod through the first opening when the illumination source is switched on.
  • the accommodation cells are provided with a second opening on a second side, opposite to the observable side, of the multi-cellular accommodation module 9, and the illumination source is configured for illuminating the arthropods through the second opening when the illumination source is switched on, so that the image formed through the first opening is a transmission image.
  • the camera may comprise a matrix of detectors, each detector of the matrix having a detector first extension in the first direction, and a detector second extension in the second direction. More particularly, the detector first extension and/or second extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
  • the position of the multicellular accommodation module 9 in the first direction and/or in the second direction is controlled with a precision which is better than a camera resolution respectively in the first and the second directions corresponding respectively to the first detector extension and/or to the second detector extension.
  • the camera may also be further configured to image the portion of the multicellular accommodation module 9 with only bioluminescent illumination from the arthropod, when the arthropod is bioluminescent, thus providing a bioluminescent image of the portion of the multicellular accommodation module 9.
  • the arthropod becomes luminescent when it has been fed with food mixed with bioluminescent material.
  • the small distance between the objective of the camera and the portion of the multicellular accommodation module 9 is appropriate to optimise light collected, and is thus particularly adapted to bioluminescent imaging considering that the amount of light emitted by the sample in that configuration can be very low.
  • the bioluminescent image of the portion of the multicellular accommodation module may be superposed onto the transmission image of the portion of the multicellular accommodation module obtained with an illumination source switched on, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
  • the device 1 may further comprise:
  • a first filter-holder carousel configured to place a spectral filter between an illumination source and the portion of the multicellular accommodation module 9, when the illumination source is switched on;
  • a second filter-holder carousel configured to place a spectral filter between the portion of the multicellular accommodation module 9 and the camera, when the illumination source is switched off, and when the at least one arthropod is bioluminescent.
  • multispectral versions of the transmission/bioluminescent images of the portion of the multicellular accommodation module may be formed on the camera and may be superposed onto one another, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
  • the invention relates to a system for optical imaging of at least one arthropod, the system comprising a device according to anyone of the embodiments described herein above, and the multi-cellular accommodation module 9.

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Abstract

Device (1) for optical imaging of at least one arthropod, the at least one arthropod being accommodated in an accommodation cell of a multi-cellular accommodation module (9), the accommodation cell being provided with a first opening on an observable side of the multi-cellular accommodation module (9), the device (1) comprising: - a camera configured to image the whole, or a portion, of the observable side of the multicellular accommodation module (9), said portion comprising the first opening of the accommodation cell, an image of the at least one arthropod being formed through the first opening; - a position of the camera with respect to the observable side of the multicellular accommodation module (9) being adjustable so that a distance between an objective of the camera and a central point of the portion of the multicellular accommodation module (9) may be set to any value between a minimum value and a maximum value.

Description

DESCRIPTION
TITLE : Device for optical imaging of arthropods.
TECHNICAL FIELD OF THE INVENTION
This invention relates to the field of monitoring arthropods.
BACKGROUND
There is a need for a compact device configured to provide images of arthropods individually hosted and fed in multi-cellular accommodation modules, the images being formed under different conditions of illumination, before and/or after feeding, so that different images may be superposed to one another for analysis.
SUMMARY OF THE INVENTION
To that end, the present invention provides a device for optical imaging of at least one arthropod, the at least one arthropod being accommodated in an accommodation cell of a multi-cellular accommodation module, the accommodation cell being provided with a first opening on an observable side of the multi-cellular accommodation module, the device comprising:
- a camera configured to image the whole, or a portion, of the observable side of the multicellular accommodation module, said portion comprising the first opening of the accommodation cell, an image of the at least one arthropod being formed through the first opening;
- a position of the camera with respect to the observable side of the multicellular accommodation module being adjustable so that a distance between an objective of the camera and a central point of the portion of the multicellular accommodation module may be set to any value between a minimum value and a maximum value.
According to an embodiment, the invention comprises one or more of the following features, alone or in any combination technically compatible.
According to an embodiment, the minimum value is comprised between 0 cm and 5 cm, in particular between 0 cm and 1 cm, more particularly between 0 mm and 1 mm.
According to these provisions, the distance between the objective of the camera can be chosen very small and the device is compact and nevertheless configured to provide high resolution images of the at least one arthropod, especially with high magnification objectives, typically with magnifications comprised between x0,1 and x10, in particular with magnifications comprised between x1 and x5, more particularly with a magnification equal to x4.
According to an embodiment, the device comprises an illumination source configured for illuminating the at least one arthropod through the first opening when the illumination source is switched on;
According to an embodiment, the accommodation cell is provided with a second opening on a second side, opposite to the observable side, of the multi-cellular accommodation module, and the illumination source is configured for illuminating the at least one arthropod through the second opening when the illumination source is switched on, so that the image formed through the first opening is a transmission image.
According to an embodiment, the device comprises a transparent support, the transparent support being movable with respect to the camera, and wherein the observable side of the multicellular accommodation module is lying on and removably attached to the transparent support, the portion of the multicellular accommodation module being imaged by the camera through the transparent support, said portion being determined by a position the movable transparent support. According to these provisions, the objective of the camera is configured to image the whole or any portion of the observable side of the multicellular accommodation module, depending on the position of the movable transparent support, each portion comprising different accommodation cells, each accommodation cell accommodating an individual arthropod.
According to an embodiment, the transparent support is movable according to a first direction, said first direction being parallel to the observable side of the multicellular accommodation module.
According to an embodiment, the transparent support is movable according to a second direction, said second direction being parallel to the observable side of the multicellular accommodation module.
According to an embodiment, the transparent support is a bottom of a basket configured to house the multicellular accommodation module, the device further comprising a first driving module on a first platform, the basket being movably attached to the first platform, the basket having a basket opening opposite to the bottom of the basket on one side of the first platform, the first driving module being configured to drive the translation of the basket along a first direction, the first platform comprising a first platform opening, the device further comprising a second driving module on a second platform, the second driving module being configured to drive the translation of the first platform along a second direction, the second platform comprising a second platform opening, the first platform opening and the second platform opening being superposed, wherein the basket is configured to pass through the first platform opening and the second platform opening so that the bottom of the basket, and the camera are on the same other side of the second platform.
According to these provisions, the camera, and the camera objective, may be as close as possible to the observable side of the multicellular accommodation module when the observable side of the multicellular accommodation module is lying on the bottom of the basket.
According to an embodiment, the first driving module comprises at least one motor configured to move the basket and the transparent support at the bottom of the basket, relative to the first platform, according to a translation along the first direction, from a first position of the basket to a second position of the basket, a first extension of the translation being defined by a maximum distance between the first position and the second position of the basket, wherein the first extension of the translation is determined by a dimension of the first opening along the first direction
According to an embodiment, the second driving module comprises a second motor to move the first platform, and the basket movably attached to the first platform, along the second direction, relative to the second platform, third position of the basket and a fourth position of the basket, a second extension of the translation being defined by a maximum distance between the third position and the fourth position of the basket, wherein the second extension of the translation is determined by a dimension of the second opening along the second direction.
According to these provisions, the extension of the displacement in any direction can be determined by the dimensions of the first opening and second opening, which can be adapted to the dimensions of the multicellular accommodation module.
According to an embodiment, the position of the transparent support along the first or the second direction is controlled with a precision less 2 pm, the precision being preferably less than 1 pm.
According to these provisions, the position of the multicellular accommodation module along the first or the second direction is controlled with the same precision, i.e. a precision less 2 pm, the precision being preferably less than 1 pm.
According to an embodiment, a first extension of the multicellular accommodation module along the first direction is comprised between 0 and 200 mm, preferably between 0 and 100 mm, more preferably between 0 and 50 mm. According to an embodiment, a second extension of the multicellular accommodation module along the second direction is comprised between 0 and 200 mm, preferably between 0 and 100 mm, more preferably between 0 and 50 mm.
According to an embodiment, a first extension of the movement of the multicellular accommodation module along the first direction is equal to the first extension of the multicellular accommodation module along the first direction.
According to an embodiment, a second extension of the movement of the multicellular accommodation module along the second direction is equal to the second extension of the multicellular accommodation module along the second direction.
According to these provisions, the portion of the multicellular accommodation module being imaged by the camera can be determined according to the precision of the position of the movable transparent support.
According to these provisions, the camera of the device can image any portion of the multicellular accommodation module. In particular, the camera of the device can image any portion of a specific multicellular accommodation module having extensions in the first and in the second directions corresponding to a standard format comprising, for example 96 or 144 cells in both direction.
In practice, along the first or along the second directions, the number of cells of the multicellular accommodation module may be any number, provided the number of cells multiplied by the dimension of each cell respectively along the first or along the second direction fits with the extension of the multicellular accommodation module respectively along the first direction or along the second direction.
According to an embodiment, the camera comprises a matrix of detectors, each detector of the matrix having a detector first extension in the first direction, and a detector second extension in the second direction.
According to an embodiment, the detector first extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
According to an embodiment, the detector second extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
According to these provisions, the position of the multicellular accommodation module in the first direction and/or in the second direction is controlled with a precision which is better than a camera resolution respectively in the first and the second directions corresponding respectively to the first detector extension and/or to the second detector extension.
According to an embodiment, the at least one arthropod is bioluminescent, and wherein the camera is further configured to image the portion of the multicellular accommodation module with only bioluminescent illumination from the arthropod, thus providing a bioluminescent image of the portion of the multicellular accommodation module.
The small distance between the objective of the camera and the central point of the portion of the multicellular accommodation module is appropriate to optimise light collected, which is particularly adapted to bioluminescent imaging considering that the amount of light emitted by the sample can be very low.
According to these provisions, the bioluminescent image of the portion of the multicellular accommodation module may be superposed onto the transmission image of the portion of the multicellular accommodation module obtained with an illumination source switched on, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions. According to an embodiment, the device further comprises :
- a first filter-holder carousel configured to place a spectral filter between an illumination source and the portion of the multicellular accommodation module, when the illumination source is switched on;
- a second filter-holder carousel configured to place a spectral filter between the portion of the multicellular accommodation module and the camera, when the illumination source is switched off, and when the at least one arthropod is bioluminescent.
According to these provisions, multispectral versions of the transmission/bioluminescent images of the portion of the multicellular accommodation module may be formed on the camera and may be superposed onto one another, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
According to another aspect, the invention provides a system for optical imaging of at least one arthropod, the system comprising a device according to anyone of the embodiments dexcribed hereinabove, and the multi-cellular accommodation module.
BRIEF DESCRIPTION OF THE DRAWINGS
The foregoing and other purposes, features, aspects and advantages of the invention will become apparent from the following detailed description of embodiments, given by way of illustration and not limitation with reference to the accompanying drawings, in which the same reference refer to similar elements or to elements having similar functions, and in which:
[Fig. 1] is an exploded perspective view of the device according to the invention.
DETAILED DESCRIPTION OF THE INVENTION ACCORDING TO AN EMBODIMENT
An embodiment of the device 1 according to the invention will be described in reference to figure 1 .
The device 1 is configured to embed a multi-cellular accommodation module 9, comprising at least one accommodation cell, preferably a plurality of accommodation cells, each of the accommodation cells being configured to host an arthropod; the accommodation cells are provided with a first opening on an observable side of the multi-cellular accommodation module 9 : for example, on the figure the observable side of the multi-cellular accommodation module 9 is the downward side, lying on a transparent support 8.
The device 1 comprises a camera, not represented on the figure, configured to observe the observable side of the multi-cellular accommodation module 9 through the transparent support 8. For example on figure 1 , the camera is attached to the device 1 and configured to observe from below the observable side of the multi-cellular accommodation module 9, in the direction D of observation towards the observable side of the multi-cellular accommodation module 9.
The camera is further configured to image the whole, or a portion, of the observable side of the multicellular accommodation module 9.
The camera may be attached to the device 1 and the transparent support 8 is configured to be movable with respect to the camera, so that when the transparent support 8 is displaced with respect to the attached camera, the imaged portion of the observable side of the multicellular accommodation module 9 is displaced accordingly.
The camera may be attached to the device 1 so that its position with respect to the observable side of the multicellular accommodation module 9 is adjustable in a direction of observation D; thus, a distance between an objective of the camera and a central point of the observed portion of the multicellular accommodation module 9 may be set to any value between a minimum value and a maximum value.
As an example, the minimum value is comprised between 0 cm and 5 cm, in particular between 0 cm and 1 cm, more particularly between 0 mm and 1mm; according to these provisions, the distance between the objective of the camera can be chosen very small and the device is compact and nevertheless configured to provide high resolution images of the at least one arthropod hosted in the cell of the observed portion of the multicellular accommodation module 9, especially with high magnification objectives; typically magnifications may be comprised between x0,1 and x10, in particular comprised between x1 and x5, more particularly magnification is equal to x4.
As an example, the transparent support 8 may be the bottom of a basket 8, the basket 8 being attached to a frame 2, said frame 2 being movably attached to at least one longitudinal rail 7, so that the movement of the frame 2 may is guided along the at least one longitudinal rail 7. A motor 4 driving a belt 6, via a pulley system 5, may be configured to drive the translation of the frame 2 in a first direction YY’, along the at least one longitudinal rail 7; according to these provisions, a position of the frame 2, consequently a position of the observed portion of the multicellular accommodation module 9 relative to the camera may be determined precisely along the at least one longitudinal rail 7. In particular, the position of the observed portion of the observable side of the multicellular accommodation module 9 may be controlled in the first direction YY’ with a precision less 2 pm, the precision being more particularly less than 1 pm.
The first driving module, comprising a first platform 3, the motor 4, the at least one driving belt 6 and the at least one longitudinal rail 7, the motor 4, and the at least one longitudinal rail 7 being both attached on the first platform 3, the first driving module being thus configured to drive, via the pulley system 5 and the driving belt 6, the translation movement of the frame 2 and, consequently of the observable portion of the multicellular accommodation module 9, along a first direction, with respect to the camera which is configured to be fixed along the first direction.
The first driving module is itself translated by a second driving module, along a second direction XX’, transversal to the first direction YY’, the second driving module comprising a second platform 10, with a second motor 4’, a second belt 6’, and a second pulley system 5’, and at least one other longitudinal rail 7’, the second motor 4’ being configured to drive the second belt 6’, via the second pulley system 5’, along the at least one other longitudinal rail 7’; thus a position of the observed portion of the multicellular accommodation module 9 relative to the camera may be determined precisely along the second direction XX’.
According to these provisions, the first platform 3, with the first driving module, is movable along the second direction XX’, relative to the second platform 10, which is fixed, the camera being fixed with respect to the second platform 10, in the first and in the second direction YY’ and XX’; as indicated hereinabove, the position of the camera in the direction of observation D is adjustable so that near-field imaging of the observable portion of the multicellular accommodation module 9 may be performed, at any position of said portion along the first and/or along the second direction YY’ and XX’.
According to these provisions, a position of the observed portion of the observable side of the multicellular accommodation module 9 may be controlled in the first direction YY’ and/or in the second direction XX’ with a precision less 2 pm, the precision being more particularly less than 1 pm.
In particular, the first platform 3 of the first driving module comprises a first platform 3 opening, and the second platform 10 of the second driving module comprises a second platform 10 opening; the first platform 3 opening and the second platform 10 opening are superposed; the basket 8, attached to the frame 2 which is movably attached on one side of the first platform 3, is configured to pass through the first platform 3 opening and the second platform 10 opening so that the multicellular accommodation module 9 lying on the bottom of the basket 8 and the camera are on the same other side of the second platform 10.
According to these provisions, the camera, more particularly the camera objective may be as close as possible to the observable side of the multicellular accommodation module 9.
The basket 8, the bottom of which is transparent and configured to support the multicellular accommodation module 9, may have any required dimensions, in particular to lodge multicellular accommodation modules 9 which may comprise 96 or 144 cells in the first YY’ and/or in the second XX’ directions. Accordingly, an extension of the multicellular accommodation module, consequently of the basket 8, along the first direction YY’, or in the second direction XX’, may be comprised between 0 and 200 mm, in particular between 0 and 100 mm, more particularly between 0 and 50 mm, depending on the number of cells and on the dimension of each cell respectively along the first or along the second direction.
The device 1 may in particular comprise an illumination source, not represented on figure 1 , the illumination source being configured for illuminating the at least one arthropod through the first opening when the illumination source is switched on.
More particularly, the accommodation cells are provided with a second opening on a second side, opposite to the observable side, of the multi-cellular accommodation module 9, and the illumination source is configured for illuminating the arthropods through the second opening when the illumination source is switched on, so that the image formed through the first opening is a transmission image.
For example, the camera may comprise a matrix of detectors, each detector of the matrix having a detector first extension in the first direction, and a detector second extension in the second direction. More particularly, the detector first extension and/or second extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
According to these provisions, the position of the multicellular accommodation module 9 in the first direction and/or in the second direction is controlled with a precision which is better than a camera resolution respectively in the first and the second directions corresponding respectively to the first detector extension and/or to the second detector extension.
The camera may also be further configured to image the portion of the multicellular accommodation module 9 with only bioluminescent illumination from the arthropod, when the arthropod is bioluminescent, thus providing a bioluminescent image of the portion of the multicellular accommodation module 9. The arthropod becomes luminescent when it has been fed with food mixed with bioluminescent material.
The small distance between the objective of the camera and the portion of the multicellular accommodation module 9 is appropriate to optimise light collected, and is thus particularly adapted to bioluminescent imaging considering that the amount of light emitted by the sample in that configuration can be very low.
According to these provisions, the bioluminescent image of the portion of the multicellular accommodation module may be superposed onto the transmission image of the portion of the multicellular accommodation module obtained with an illumination source switched on, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions.
The device 1 may further comprise:
- a first filter-holder carousel configured to place a spectral filter between an illumination source and the portion of the multicellular accommodation module 9, when the illumination source is switched on;
- a second filter-holder carousel configured to place a spectral filter between the portion of the multicellular accommodation module 9 and the camera, when the illumination source is switched off, and when the at least one arthropod is bioluminescent.
According to these provisions, multispectral versions of the transmission/bioluminescent images of the portion of the multicellular accommodation module may be formed on the camera and may be superposed onto one another, the precision of the superposition being appropriately improved by the precision of the determination of the position of the multicellular accommodation module along first and second directions and by the camera resolution in the first and the second directions. According to an aspect, the invention relates to a system for optical imaging of at least one arthropod, the system comprising a device according to anyone of the embodiments described herein above, and the multi-cellular accommodation module 9.

Claims

8 CLAIMS
1. Device (1) for optical imaging of at least one arthropod, the at least one arthropod being accommodated in an accommodation cell of a multi-cellular accommodation module (9), the accommodation cell being provided with a first opening on an observable side of the multicellular accommodation module (9), the device (1) comprising:
- a camera configured to image the whole, or a portion, of the observable side of the multicellular accommodation module (9), said portion comprising the first opening of the accommodation cell, an image of the at least one arthropod being formed through the first opening;
- a position of the camera with respect to the observable side of the multicellular accommodation module (9) being adjustable so that a distance between an objective of the camera and a central point of the portion of the multicellular accommodation module (9) may be set to any value between a minimum value and a maximum value.
2. Device (1) according to claim 1 , comprising a transparent support (8), the transparent support (8) being movable with respect to the camera, and wherein the observable side of the multicellular accommodation module (9) is lying on and removably attached to the transparent support (8), the portion of the multicellular accommodation module (9) being imaged by the camera through the transparent support (8), said portion being determined by a position the movable transparent support (8).
3. Device (1) according to anyone of the preceding claims, wherein the transparent support (8) is movable according to a first direction, said first direction being parallel to the observable side of the multicellular accommodation module (9).
4. Device (1) according anyone of the preceding claims, wherein the transparent support (8) is movable according to a second direction, said second direction being parallel to the observable side of the multicellular accommodation module (9).
5. Device (1) according to anyone of the preceding claims, wherein the transparent support (8) is a bottom of a basket (8) configured to house the multicellular accommodation module (9), the device (1) further comprising a first driving module on a first platform (3), the basket (8) being movably attached to the first platform (3), the basket (8) having a basket opening opposite to the bottom of the basket (8) on one side of the first platform (3), the first driving module being configured to drive the translation of the basket (8) along a first direction (YY’), the first platform (3) comprising a first platform (3) opening, the device further comprising a second driving module on a second platform (10), the second driving module being configured to drive the translation of the first platform (3) along a second direction (XX’), the second platform (10) comprising a second platform (10) opening, the first platform (3) opening and the second platform (10) opening being superposed, wherein the basket (8) is configured to pass through the first platform (3) opening and the second platform (10) opening so that the bottom of the basket (8), and the camera are on the same other side of the second platform (10).
6. Device (1) according to anyone of the claims 3 or 4, wherein the camera comprises a matrix of detectors, each detector of the matrix having a detector first extension in the first direction, and a detector second extension in the second direction.
7. Device (1) according to claim 5, wherein the detector first extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
8. Device (1) according to claim 5 or 6, wherein the detector second extension is less or equal to 20 pm, preferably less or equal than 15 pm, more preferably less or equal than 10 pm.
9. Device (1) according anyone of the preceding claims, wherein the at least one arthropod is bioluminescent, and wherein the camera is further configured to image the portion of the multicellular accommodation module (9) with only bioluminescent illumination from the arthropod, thus providing a bioluminescent image of the portion of the multicellular accommodation module (9). 9 evice (1) according anyone of the preceding claims, further comprising
- a first filter-holder carousel configured to place a spectral filter between an illumination source and the portion of the multicellular accommodation module (9), when the illumination source is switched on; - a second filter-holder carousel configured to place a spectral filter between the portion of the multicellular accommodation module (9) and the camera, when the illumination source is switched off, and when the at least one arthropod is bioluminescent. ystem for optical imaging of at least one arthropod, the system comprising a device according to anyone of claims 1 to 9, and the multi-cellular accommodation module (9).
PCT/EP2021/084336 2020-12-07 2021-12-06 Device for optical imaging of arthropods WO2022122628A2 (en)

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JP3787631B2 (en) * 2003-03-07 2006-06-21 国立大学法人名古屋大学 Bioluminescence measurement / analysis program, computer-readable recording medium storing the program, and bioluminescence measurement / analysis apparatus including the program and the computer
US7646482B2 (en) * 2007-05-31 2010-01-12 Genetix Limited Methods and apparatus for optical analysis of samples in biological sample containers
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