WO2022039584A1 - Cancer cell transformation observation system using circulation-type cancer cell transformation measuring device and device for collecting transformed cancer cells comprising artificial blood vessel unit - Google Patents

Cancer cell transformation observation system using circulation-type cancer cell transformation measuring device and device for collecting transformed cancer cells comprising artificial blood vessel unit Download PDF

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Publication number
WO2022039584A1
WO2022039584A1 PCT/KR2021/095077 KR2021095077W WO2022039584A1 WO 2022039584 A1 WO2022039584 A1 WO 2022039584A1 KR 2021095077 W KR2021095077 W KR 2021095077W WO 2022039584 A1 WO2022039584 A1 WO 2022039584A1
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Prior art keywords
unit
cancer cells
transformation
cancer cell
medium
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PCT/KR2021/095077
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French (fr)
Korean (ko)
Inventor
김백길
조남훈
장연수
강숙희
Original Assignee
연세대학교 산학협력단
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Priority claimed from KR1020200103130A external-priority patent/KR102502401B1/en
Priority claimed from KR1020200103122A external-priority patent/KR102420392B1/en
Application filed by 연세대학교 산학협력단 filed Critical 연세대학교 산학협력단
Publication of WO2022039584A1 publication Critical patent/WO2022039584A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the present invention relates to a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same, and more particularly, observing a phenomenon in which cancer cells are transformed as they pass through a blood vessel wall. It relates to a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells, and a cancer cell transformation observation system using the same.
  • a transformed cancer cell collection device comprising a pseudovascular unit and a transformation observation system for cancer cells using the same, and more particularly, it is possible to observe transformation of cancer cells passing through vascular endothelial cells by collecting various samples. It relates to a device for collecting transformed cancer cells including a mimetic vascular unit and a system for observing transformation of cancer cells using the same.
  • Cancer cells move through blood vessels away from the original site of origin, colonize in another organ, and metastasize to another organ.
  • cancer cells that have escaped from the original site can move through blood vessels to other organs, pass through the blood vessel wall, and move to metastases.
  • cancer cells in which the cancer cells have passed through the blood vessel wall and moved to the metastasis may have similar traits to the cancer cells of the original origin, but some cancer cells are transformed and converted into cancer cells of a different type from the cancer cells of the original origin. Circumstances may arise that require the use of other therapeutic agents.
  • the present invention is intended to solve the above problems, and an object of the present invention is to be able to observe transformation of cancer cells passing through vascular endothelial cells by collecting various samples.
  • the epicenter is divided into an outer tube and an inner tube in which the cancer cells are accommodated. part, a supply part communicating with the outer cylinder to provide a flow path for the medium to pass through the round part, a moving part coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells move, and the inner cylinder and the moving part provided between the moving part. and an induction part that secretes an inducer so that the cancer cells floating inside the round part move toward the moving part by the flow of the medium.
  • the induction part is characterized in that it is formed of a plurality of membranes through which the cancer cells can pass so that the separated cancer cells are moved to the moving part.
  • the induction part may include a first encapsulation member formed on one surface of the round part, a second encapsulation member provided to be in contact with the first encapsulation member, and a space provided between the first encapsulation member and the second encapsulation member so that the cancer cells are It guides to move to the moving part, characterized in that it comprises a imitation blood vessel inner skin material that forms an endothelial layer similar to blood vessels inside the human body.
  • the imitation vascular endothelial material is formed of vascular endothelial cells so that the cancer cells can pass through, is attached to the first encapsulation member, and secretes an inducer to move the cancer cells to the moving part, and the vascular endothelium and the vascular endothelium. It characterized in that it comprises a hydrogel layer provided between the layer and the second encapsulation member to prevent the vascular endothelial layer from being damaged by the pressure of the second encapsulation member.
  • the circular part characterized in that the outer cylinder and the inner cylinder are arranged to be spaced apart a predetermined distance.
  • the inner cylinder is characterized in that the opening is formed toward the upper side so that the medium flowing into the outer cylinder through the supply unit is introduced into the inside.
  • the inner cylinder is characterized in that the medium is introduced and a filtering member is provided in the opening to prevent the cancer cells from leaving the opening.
  • the moving unit may include a guide member for guiding the movement of the cancer cells between the guide unit and the outer cylinder so that the cancer cells can be moved to the moving unit.
  • the guide member is characterized in that it extends from the periphery of the guide part toward the moving part so that the diameter becomes narrower.
  • the circulating cancer cell transformation measuring device including the same is a measuring unit for inducing transformation of cancer cells to observe the transformation of cancer cells that have passed through the endothelium.
  • a round part divided into an inner cylinder in which the cancer cells are accommodated, a supply part communicating with the outer cylinder to provide a flow path for the medium to pass through the round part, a moving part coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells are moved, and the A measuring unit for inducing transformation of cancer cells, which is provided between the inner passage and the moving unit and includes an inducing unit that secretes an inducer so that the cancer cells floating inside the round part are moved to the moving unit by the flow of the medium; and and a circulation unit communicating with the moving unit and providing a flow path for circulating the cancer cells and the medium moving along the moving unit to the circular unit.
  • the circulation unit is provided on the flow path and characterized in that it includes a collecting unit for collecting the cancer cells moving through the moving unit.
  • the collection unit replaces a part of the flow path, and is provided between the extraction member and the extraction member formed of a plurality of membranes so that the cancer cells are extracted, and secretes an inducer to promote the movement of the cancer cells, and the cancer cells are It is characterized in that it comprises a facilitating member for facilitating extraction to the outside through the extraction member.
  • the circulation unit characterized in that it comprises a circle portion in which the flow of the medium and the cancer cells is divided into at least one or more flow passages.
  • the circle portion has a radius of curvature and is bent to form a closed loop in which the divided flow paths are integrated.
  • the cancer cell transformation observation system using the above-described circulating cancer cell transformation measuring device is a measurement unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium, and is divided into an outer passage and an inner tube in which cancer cells are accommodated.
  • a circular part to be in communication with the outer cylinder to provide a flow path for the medium to pass through the circular part
  • a moving part coupled to one surface of the inner cylinder to provide a flow path for the cancer cells to move
  • a plurality of measurement units for inducing transformation of cancer cells and communicating with the plurality of measurement units, which includes an induction unit that secretes an inducer so that the cancer cells floating inside the round cell are moved to the moving unit by the flow of the medium
  • the circulation unit is provided on the flow path and characterized in that it includes a collecting unit for collecting the cancer cells moving through the moving unit.
  • the collection unit replaces a part of the flow path, and is provided between the extraction member and the extraction member formed of a plurality of membranes so that the cancer cells are extracted, and discharges an inducing material that promotes the movement of the cancer cells, and the cancer cells It is characterized in that it comprises a facilitating member that promotes extraction to the outside through the extraction member.
  • the inducing material secreted by the induction unit and the cancer cells are exchanged with each other through the circulation unit.
  • the circulation unit is characterized in that it includes a circle portion having a closed loop having a curvature from one side toward the other side and being bent so that one side and the other side are connected to each other.
  • an inlet and outlet are formed, and in order to observe transformation of cancer cells passing through the blood vessel wall, a pseudovascular unit is formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet.
  • a ring portion disposed along a path to be spaced apart to form a gap spaced apart so that the cancer cells are discharged to the outside, a plurality of the ring portions are positioned therein, and a plurality of membranes are formed so that the cancer cells flow out from the spaced gap It secretes an inducer as much as possible, and includes a membrane portion serving as a path through which the medium and the cancer cells move.
  • a portion of the periphery of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
  • the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
  • connecting portion connecting the ring portion adjacent in the spaced gap, characterized in that it maintains the spacing of the plurality of the ring portion.
  • the connecting portion is characterized in that a plurality is formed along the circumference of the ring portion.
  • the film portion, the cover member provided to surround the ring portion and the connection portion, is formed along the circumference of the cover member, characterized in that it comprises a secretion member that secretes an inducer to move the cancer cells to the outside. do.
  • the secretory member is characterized in that it is composed of vascular endothelial cells.
  • the film portion characterized in that it further comprises a moisture retention member provided along the circumference of the secretion member so that the moisture of the secretion member is maintained.
  • the moisture retention member is formed along the circumference of the secretion member between the ring parts, characterized in that it is formed to surround the secretion member.
  • the ring portion in contact with the inner periphery of the film portion, characterized in that for supporting the film portion.
  • an inlet and an outlet are formed, and in the inlet to observe transformation of cancer cells passing through the blood vessel wall,
  • a imitation vascular unit formed in a portion of a path through which the medium and cancer cells move toward the outlet, a ring portion disposed to be spaced apart on the path to form a gap so that the cancer cells are discharged to the outside, a plurality of the rings inside
  • An imitation vascular unit having an additional location, formed of a plurality of membranes, secreting an inducer to allow the cancer cells to flow out from the gap, and including a membrane portion in which the medium and a path through which the cancer cells move are formed, a space inside Formed, the cancer cells are located, and the primary unit and the imitation vascular unit are disposed in communication with the inlet and the outlet to circulate the medium, and collect the cancer cells moving from the imitation vascular unit to the outside Includes a receiving unit.
  • the receiving unit is characterized in that the inducer is accommodated to promote the movement of the cancer cells moving through the pseudovascular unit to the outside.
  • a circulation part connected to the inlet and the outlet to circulate the medium, an external housing communicating with the circulation part and spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top
  • the propulsion part is divided into an inner housing that becomes an inner housing, which secretes an inducing material so that the cancer cells floating in the inner housing move toward the pseudovascular unit by the circulation of the medium, and promotes formation of a plurality of membranes on one surface of the inner housing It is characterized by including wealth.
  • the facilitating part is provided between the first upper skin material formed on one surface of the propulsion part, the second upper skin material provided to be in contact with the first film, and the first film and the second film to secrete an inducer. It is characterized in that it comprises a third upper skin material.
  • the third epidermal material is provided with vascular endothelial cells so that the cancer cells are moved toward the imitation vascular unit, and the imitation film layer is attached to the first epidermal material, and the imitation by the first epidermis and the second epidermis. It characterized in that it comprises a substrate layer provided between the imitation film layer and the second upper skin material in order to prevent the film layer from being damaged.
  • the inner housing characterized in that it includes a filtering member provided in the opening in order to prevent the cancer cells from escaping to the outside.
  • the imitation blood vessel unit a portion of the circumference of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
  • the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
  • the system for observing transformation of cancer cells using the transformed cancer cell collection device including the above-described pseudovascular unit, an inlet and an outlet are formed, and observe the transformation of cancer cells passing through the blood vessel wall.
  • a imitation vascular unit formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet a plurality of vascular units are arranged to be spaced apart on the path to form a gap so that the cancer cells are discharged to the outside, inside
  • a imitation vascular unit comprising a membrane portion in which a plurality of the ring portions are located, a plurality of membranes are formed to secrete an inducer to allow the cancer cells to flow out from the gap, and a membrane portion in which the medium and a path through which the cancer cells move are formed;
  • a space is formed inside, the cancer cells are located, and the primary unit and the imitation vascular unit in which the medium is circulated in communication with the inlet and the outlet are disposed while penetrating the inside, and the imitation vascular unit is
  • the extraction device is characterized in that the inner space is formed to be stepped, and a space is formed in the lower part so that the cancer cells are precipitated in the inner space.
  • the circulation device is characterized in that it includes a pump unit for providing power to move the inducer on the movement path of the inducer.
  • a circulation part connected to the inlet and the outlet to circulate the medium, an external housing communicating with the circulation part and spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top
  • the propulsion part is divided into an inner housing that becomes an inner housing, which secretes an inducing material so that the cancer cells floating in the inner housing move toward the pseudovascular unit by the circulation of the medium, and promotes formation of a plurality of membranes on one surface of the inner housing It is characterized by including wealth.
  • the facilitating unit may include a first upper skin material formed on one surface of the propulsion part, a second upper skin material provided to be in contact with the first film, and a second upper skin material provided between the first film and the second film to secrete an inducer. 3 It is characterized in that it includes an upper skin material.
  • the third epidermal material is provided with vascular endothelial cells so that the cancer cells are moved toward the imitation vascular unit, and the imitation coating layer is attached to the first epidermis, and the first epidermis and the second epidermis.
  • the imitation coating layer In order to prevent the imitation coating layer from being damaged, it characterized in that it comprises a substrate layer provided between the imitation coating layer and the second upper skin material.
  • the imitation blood vessel unit a portion of the circumference of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
  • the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
  • the transformation of cancer cells passing through vascular endothelial cells can be performed by collecting various samples. There is an observable effect.
  • FIG. 1 is a view showing the overall appearance of a measuring unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 2 is a view illustrating an induction unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 3 is a diagram illustrating the flow of the medium and cancer cells in the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention. drawing;
  • FIG. 4 is a view for explaining a measuring device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 5 is a view illustrating a collection unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 6 is a view for explaining the circulation unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention
  • FIG. 7 is a diagram illustrating the situation inside the measuring unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention. drawing;
  • FIG. 8 illustrates a situation in which a plurality of measuring units are provided in a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention.
  • FIG. 9 is a diagram illustrating the relationship between a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 10 is a diagram illustrating an observation system of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 11 is an overall view of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit, according to an embodiment of the present invention, including a dummy vascular unit. drawing;
  • FIG. 12 is a view for explaining the imitation vascular unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention
  • FIG. 13 is a view for explaining the capsule part of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention
  • FIG. 14 is a view illustrating a primary unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 15 is a view for explaining a facilitating unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • 16 is a view for explaining an extraction device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 17 is a view showing the overall flow of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit according to an embodiment of the present invention, in a state including the imitation vascular unit; drawings for the purpose of explanation;
  • FIG. 18 is a diagram illustrating a situation in which cancer cells flow out through a dummy vascular unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention.
  • FIG. 19 is a more detailed view of cancer cells in the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention A drawing shown to explain;
  • 20 is a view showing that a reinforcement part is formed in the imitation blood vessel unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention; It is a drawing.
  • FIG. 1 is a view showing the overall appearance of a measuring unit of a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention
  • FIG. 2 is a diagram illustrating a circulating cancer cell transformation measuring apparatus including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an induction unit of a cancer cell transformation observation system using the same.
  • the measuring unit for inducing transformation of cancer cells is the epicenter 100 , and the supply unit 200 providing a flow path for the medium to pass through the epicenter 100 .
  • the supply unit 200 may be composed of an induction unit 300 that secretes an inducer so that the cancer cells C move toward the moving unit 400 and a moving unit 400 that provides a flow path through which the cancer cells C move.
  • the round part 100 is arranged to be separated from the outer cylinder 120 and the outer cylinder 120, and is spaced apart from the outer cylinder 120 by a predetermined distance and consists of an inner cylinder 140 in which the cancer cells C are accommodated.
  • the inner tube 140 may have an opening upward so that the medium can be introduced, and the cancer cells C floating in the inner tube 140 by the flow of the medium flowing through the opening In order to prevent escape through the opening, the medium can freely move through the opening, but there is a filtering member in the opening to limit the escape of the cancer cells (C) so that the cancer cells (C) are isolated to the inner tube (140). 142 may be formed.
  • the supply unit 200 communicates with the outer cylinder 120 so that the medium can be supplied toward the inside of the round section 100 so that the medium can be moved through the round section 100 . It is communicated with the upper part of the outer cylinder 120 may supply the medium.
  • the supply unit 200 may be located above the height of the inner cylinder 140 and communicate with the outer cylinder 120, and the medium flowing into the outer cylinder 120 from the supply unit 200 is As the area of the flow path increases, the flow rate may be slowed to allow the flow of the medium to flow in the lower direction at a low speed.
  • the induction unit 300 is coupled to one surface of the inner tube 140, the cancer cells (C) floating inside the inner tube 140 by the flow of the medium is moved toward the moving unit 400. It is formed of a plurality of membranes through which the cancer cells (C) can pass, and as shown in FIG. 2 , the first encapsulation member 320 , the second encapsulation member 340 , and the imitation blood vessel inner skin member 360 are formed. may include
  • the first coating member 320 may be formed on one surface of the inner cylinder 140 .
  • the second encapsulation member 340 may be provided between the first encapsulation member 320 and the moving part 400 so as to be in contact with the first encapsulation member 320 .
  • the imitation blood vessel inner skin material 360 secretes an inducing material so that the cancer cells C can move toward the moving unit 400 , and the first encapsulation member 320 and the second encapsulation member It is provided between the 340 to induce the movement of the cancer cells (C).
  • the imitation vascular inner skin material 360 may form an endothelial layer similar to a blood vessel inside the human body so that the cancer cells (C) can be transformed while passing through the vascular endothelium.
  • the imitation vascular endothelial skin material 360 is formed of vascular endothelial cells to allow the cancer cells (C) to pass through, is attached to the first encapsulation member 320, and the cancer cells (C) are moved to the moving part (
  • the vascular endothelial layer 362 is composed of vascular endothelial cells so that it can be observed more effectively that the cancer cells (C) are transformed while passing through the vascular cells inside the human body, and the inducer secreted so that the cancer cells C are moved toward the moving unit 400 .
  • the hydrogel layer 364 is provided between the vascular endothelial layer 362 and the second encapsulation member 340 to prevent the vascular endothelial layer 362 from being damaged, and the cancer cells C are moved.
  • a plurality of pores are formed so that the cells can be transformed, and by creating an environment similar to the extracellular matrix inside the human body, it is possible to create an environment more similar to that the cancer cells (C) pass through the vascular cells and are transformed.
  • the moving unit 400 is coupled to one surface of the inner cylinder 140 to provide a flow path through which the cancer cells C passing through the induction unit 300 can move, and the induction unit 300 and A guide member 420 for guiding the cancer cells C between the outer cylinder 120 to be moved along the movement direction B through the guide part 300 may be included.
  • the guide member 420 may extend from the inner tube 140 toward the outer tube 120 so that the diameter becomes narrower.
  • FIG. 3 is a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a medium and cancer cell flow of a cancer cell transformation observation system using the same. It is the drawing shown.
  • the medium passing through the round part 100 through the supply unit 200 is coupled to the upper surface of the outer cylinder 120 so that the medium flows toward the inner cylinder 140, and of the inner cylinder 140
  • the cancer cells (C) float in the inner tube 140 by the inflow of the medium, and can be prevented from being separated into the opening by the filtering member 142 .
  • the cancer cells (C) floating in the inner tube 140 by the medium move in the induction part 300 by the inducer secreted from the vascular endothelial layer 362 at a position adjacent to the induction part 300 . It can be moved to the unit 400 .
  • the cancer cells (C) are transformed while passing through the vascular endothelial layer 362, and the transformed cancer cells (C) are moved through the moving unit 400 to observe the cancer cells (C).
  • FIG. 4 is a diagram illustrating a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a measuring device of a cancer cell transformation observation system using the same
  • FIG. 5 is a diagram illustrating a collection unit of a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention.
  • the circulating cancer cell transformation measuring apparatus includes the above-described measuring unit M for inducing transformation of cancer cells, and communicates with the moving unit 400 . and may include a circulation unit 500 providing a flow path to circulate the cancer cells C and the medium moving along the moving unit 400 .
  • the measurement unit M may have the same configuration as described above, and a detailed description thereof will be omitted.
  • the circulation unit 500 communicates with the moving unit 400 to provide a flow path through which the cancer cells C and the medium are moved, and is provided in a portion of the flow path through the moving unit 400 . It may include a collecting unit 520 for collecting the moving cancer cells (C) and a circle unit 540 in which the flow path is divided into at least one flow path.
  • the collection unit 520 is provided on a part of the flow path to replace a part of the flow path so that the cancer cells C can be collected, and the extraction member 522 is formed of a plurality of membranes so that the cancer cells C are extracted. and a facilitating member 524 that promotes movement of the cancer cells C to the outside by secreting an inducer to collect the cancer cells C between the extraction members 522 and the outer circumference of the facilitating member 524 It may include a substrate member 526 formed along the facilitating member 524 to prevent damage by the extraction member (522).
  • the extraction member 522 may be divided into an outer skin layer and an endothelial layer, and may be formed in a portion of a path through which the cancer cells C and the medium are moved.
  • outer cortical layer and the inner cortical layer may have pores so that the cancer cells C can move and pass through.
  • the promotion member 524 may be formed along the outer periphery of the endothelial layer, and secrete an inducer to promote the movement of the cancer cells C to the outside.
  • the facilitating member 524 may have the same configuration as the vascular endothelial layer 362 described above, that is, may be composed of vascular endothelial cells.
  • the substrate member 526 is formed between the promotion member 524 and the outer skin layer, and may be formed similarly to the hydrogel layer 364 described above.
  • the matrix member 526 may provide an environment similar to the extracellular matrix, and a plurality of pores may be formed so that the cancer cells C can move to the outside.
  • the circle unit 540 communicates with the moving unit 400 , and may be divided into at least one flow path at a point communicating with the moving unit 400 , and the cancer cells C and the medium
  • the divided flow paths to be circulated may be integrated from one side to form a closed loop.
  • the circle portion 540 has a radius of curvature, and one end of the divided flow passages is bent toward each other to be integrated into one flow passage to form a circulation ring to form a closed loop.
  • a circulating cancer cell transformation measuring apparatus according to an embodiment of the present invention can be described with reference to FIGS. 6 to 7 .
  • FIG. 7 is a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an internal situation of a measuring unit of a cancer cell transformation observation system using the same. It is the drawing shown.
  • the cancer cells C are moved to a position adjacent to the induction unit 300 while floating in the inner tube 140 by the medium introduced through the supply unit 400 , and the induction unit The cancer cells C may be moved through the induction unit 300 toward the moving unit 400 by the inducer secreted from 300 .
  • the cancer cells C moved to the moving unit 400 may circulate a closed loop formed through the circle unit 540 as shown in FIG. 6 , and the The collection unit 520 formed in a part of the path may be moved to the outside and collected.
  • the inducing material secreted from the facilitating member 524 formed in the collection unit 520 is delivered to the cancer cells C accommodated in the inner tube 140 by the circulation of the circle unit 540, The probability of passing through the induction unit 300 may be increased, and the collection unit 520 may be more effectively moved to the outside by the induction material secreted from the induction unit 300 .
  • a separate pump member for additionally supplying an inducing material so that the cancer cells C can move toward the collecting unit 520 through the induction unit 300 is formed in a part of the circle unit 540 .
  • the cancer cell transformation observation system according to an embodiment of the present invention can be described with reference to FIGS. 8 to 10 .
  • FIG. 8 illustrates a situation in which a plurality of measuring units of a circulating cancer cell transformation measuring apparatus including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a cancer cell transformation observation system using the same are provided.
  • FIG. 9 is a diagram illustrating the relationship between a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention
  • the drawings and FIG. 10 are a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an observation system of a cancer cell transformation observation system using the same. It is a drawing shown for
  • the system for observing transformation of cancer cells includes a plurality of measurement units (M) having the configuration as described above and a flow path to communicate with the plurality of measurement units (M).
  • M measurement units
  • a plurality of branching points are formed, and a flow path is formed so that the plurality of branching points are connected to each other, and the circulation unit 500 in which the cancer cells and the medium are circulated may include.
  • the plurality of measurement units M may be arranged such that the moving unit 400 faces the circulation unit 500, and when at least two or more are provided as shown in FIG. 10, the circulation unit It may be radially arranged with respect to (500).
  • the circulation unit 500 may be the same as the above-described configuration, and a plurality of branch points through which a flow path is branched to communicate with the plurality of measurement units M1 and M2 may be formed.
  • the plurality of measurement units (M1, M2) described above may exchange the medium and the cancer cells (C) or inducers with each other through the circulation unit 500, through which the first measurement unit (M1) And the second measurement unit (M2) can promote the movement of the cancer cells (C) accommodated in the inner tube 140, through the induction unit 300 toward the moving unit (400).
  • the cancer cell is also affected by the inducing material secreted by the facilitating member 524 to each of the first and second measurement units M1 and M2 by the collection unit 520 .
  • the movement amount of (C) can be improved.
  • the movement from the source to the metastasized organ is not formed in a one-to-one correspondence like the first measurement unit M1 and the second measurement unit M2, but a plurality of organs. Since the transition of can occur, as shown in FIG. 10 , a plurality of transition sites are modeled based on one source, and the moving unit 400 is moved to the circulation unit 500 based on the circulation unit 500 . ) may be arranged radially to face.
  • the collection unit 520 collects a part of the cancer cells (C) that have passed through the induction unit 300 at a position adjacent to the moving unit 400 of each of the measurement units (M) and is transformed.
  • a sample of the cancer cell (C) may be possessed, and another transformed sample may be collected again through the collection unit 520 provided in the circulation unit 500, and as shown in FIG.
  • the cancer cells, and inducers are exchanged with each other, the cancer cells (C) that move toward the other measurement unit (M) are collected to collect a sample of the transformed cancer cells (C). there is.
  • the circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention, and a cancer cell transformation observation system using the same, observe a phenomenon in which cancer cells are transformed as they pass through a blood vessel wall. There may be an effect that can be done.
  • the circulating cancer cell transformation measurement device and the cancer cell transformation observation system using the same including a measurement unit for inducing transformation of cancer cells according to another embodiment of the present invention, collect cancer cells (C) separated from the source
  • a sensor interworking with the manager's terminal may be provided so that the state and transformation can be easily adjusted.
  • the amount of the medium flowing into the inner tube 140 may be adjusted by adjusting the amount of the medium supplied to the outer tube 120 , and accordingly, the cancer cells C floating in the inner tube 140 . ) may be changed to indirectly control the amount of cancer cells C passing through the induction unit 300 .
  • a sensor for sensing the cancer cell C may be disposed on the moving unit 400 to check the amount of the cancer cell C that has passed through the induction unit 300 in the inner tube 140 .
  • the manager's terminal controls the flow and flow rate of the medium, the amount of decrease in the cancer cells (C) accommodated in the inner tube 140 and the amount of cancer cells (C) passing through the moving unit 300 per hour It can be managed so that the movement amount can be grasped.
  • a management server capable of controlling the device may be further provided.
  • the management server may be provided with another sensor that can check the flow rate of the medium or check the supply amount of the medium, and check the floating amount and sedimentation amount of the cancer cells (C) in the inner tube 140, or the cancer cells
  • Another sensor that can check the capacity of (C) may be provided, and as described above, a sensor for checking the cancer cell (C) passing through the moving unit 400 is provided in the measuring device. It can also be managed by adjusting the amount of movement and change of cancer cells (C) and the medium.
  • FIG. 11 is an overall view of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit, according to an embodiment of the present invention, including a dummy vascular unit.
  • FIG. 12 is a diagram illustrating the imitation vascular unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention.
  • FIG. 13 is a diagram illustrating the capsule part of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention
  • 14 is a view illustrating a primary unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 15 is a view for explaining the facilitation unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention
  • FIG. 16 is It is a diagram illustrating an extraction device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a imitation blood vessel unit according to an embodiment of the present invention.
  • the transformed cancer cell collection device including the dummy vascular unit according to an embodiment of the present invention and the transformation collection and observation system for cancer cells using the same are the imitation vascular unit 600 and the primary unit 700 .
  • the receiving unit 800 , the extraction device 900 , and the circulation device 1000 may be configured, and the cancer cell C may be accommodated in the primary unit 700 .
  • the imitation vascular unit 600 moves through the injection hole I through which the cancer cells C move inside the primary unit 700 and the imitation vascular unit 600 . It may be formed in a part of the path of the outlet (O).
  • the imitation blood vessel unit 600 is arranged along the path so that a plurality of them are spaced apart from each other on the movement path of the cancer cells (C) to form a gap 622 spaced apart so that the cancer cells (C) are discharged to the outside ( 620) and a plurality of the ring parts 620 are positioned therein, are formed of a plurality of membranes, and secrete an inducer so that the cancer cells (C) are discharged to the outside in the spaced gap 622, and the medium And it may include a connecting portion 660 for connecting the coating portion 640 and the ring portion 620 that is a path through which the cancer cells (C) move.
  • a plurality of the ring portions 620 may be spaced apart from each other by being arranged from the inlet (I) toward the outlet (O), and the plurality of ring portions 620 may be spaced apart from each other by a predetermined distance. there is.
  • the ring portion 620 may be spaced apart from each other to form the spaced gap 622 so that the cancer cells C moving from the inlet I to the outlet O may flow out.
  • the ring part 620 is in contact with the inner side of the coating part 640 to prevent the coating part 640 formed of a plurality of films from stretching downward by gravity to close the coating part 640 . can support
  • the above-described coating portion 640 is formed of a plurality of membranes, a plurality of pores may be formed so that the cancer cells (C) can move to the outside, and as shown in FIG. 13 , the cover member ( 642 ), a secretion member 644 , and a moisture retention member 646 .
  • the cover member 642 is provided to surround the ring portion 620 and the connection portion 660 , and may be formed along the outer circumference of the ring portion 620 .
  • a plurality of pores may be formed so that the cancer cells C can move to the outside.
  • the secretion member 644 is an inducing material for inducing the outflow of the cancer cells (C) so that the cancer cells (C) can flow out of the gap (622) through the membrane portion (640) to the outside. can secrete
  • the secretion member 644 may be formed along the outer periphery of the cover member 642, and is formed as a vascular endothelial cell layer so that the cancer cells (C) can be observed to be transformed while passing through the blood vessel wall. there may be
  • cover member 642 and the secretion member 644 may be formed to be elongated from the inlet (I) toward the outlet (O).
  • the moisture retention member 646 is the secretion member to maintain moisture in the secretion member 644 in order to prevent the moisture of the secretion member 644 from being restricted from the movement of the cancer cells (C) is limited. It may be formed along the outer perimeter of 644 .
  • the moisture retaining member 646 is provided only between the above-described spaced gaps 622 and may be formed along the circumference of the secretion member 644, which is the spacing at which the cancer cells C are discharged. It is possible to keep the moisture in the secretion member 644 in the gap 622 so that the movement of the cancer cells C is not restricted.
  • the moisture retention member 646 may have a plurality of pores formed so that the cancer cells C can move, and is formed as a matrix layer to provide an environment similar to the extracellular matrix existing between blood vessels. .
  • the moisture retention member 646 maintains the moisture of the secretion member 644 so that the movement of the cancer cells C is not limited in the spaced gap 622 , and the spaced gap 622 .
  • An environment similar to the extracellular matrix is created to provide an environment similar to the human body for the cancer cells (C) flowing out through the may be allowed to leak.
  • the connecting portion 660 may be connected to each other by extending a portion of the circumference of the ring portion 620 toward the adjacent ring portion (620).
  • the connecting portion 660 connects the adjacent ring portions 620 to maintain a distance between the plurality of ring portions 620, and is formed elongated from the inlet (I) toward the outlet (O) to form a plurality of
  • the ring portion 620 may be fixed to be positioned on the path.
  • connection parts 660 may be formed along the circumference of the ring part 620 and face each other to improve the area of the spaced gap 622 through which the cancer cells C are discharged. It may be desirable to provide a pair for viewing.
  • the primary unit 700 in which the cancer cells C are accommodated has the inlet I and the outlet so that the cancer cells C can move to the imitation vascular unit 600 .
  • (O) is divided into a circulation unit 720 through which the medium is circulated, an outer housing 742 communicating with the circulation unit 720, and an inner housing 744 formed to be spaced apart from the outer housing 742 It is formed on one surface of the propulsion part 740 and the inner housing 744 to be composed of a facilitating part 760 that secretes an inducer so that the cancer cells C can move toward the imitation blood vessel unit 600 .
  • the circulation unit 720 may be in communication with the inlet (I) and the outlet (O), and a medium pump capable of supplying the medium to a part of the path or a pump providing the movement power of the medium is provided.
  • the propulsion part 740 may be divided into the outer housing 742 and the inner housing 744, and the outer housing 742 and the inner housing 744 may be disposed to be spaced apart from each other.
  • the outer housing 742 may be in communication with the circulation unit 720 so that the medium can be introduced, and the circulation unit 720 is communicated with the upper part of the outer housing 742 to allow the medium to flow in. while being naturally introduced into the inner housing 744 .
  • the inner housing 744 is formed to have a lower height than the outer housing 742 , an opening is formed upward to allow the medium to flow in, and the cancer cells C are accommodated therein.
  • the cancer cells (C) may be suspended in the inner housing 744 by the medium introduced into the inner housing 744 .
  • the opening of the inner housing 744 has the opening in order to prevent the cancer cells C floating inside the inner housing 744 by the medium from flowing out to the outer housing 742 through the opening.
  • a filtering member 746 for preventing the cancer cells C from leaving may be provided.
  • the filtering member 746 does not limit the movement of the medium toward the inner housing 744, but restricts the cancer cells C from leaving the inner housing 744, so that the cancer cells C ) can create an environment in which the imitation blood vessel unit 600 can be moved.
  • the promotion part 760 may be formed of a plurality of films as shown in FIG. 15 , and a first upper skin member 762 and the first upper skin member coupled to one surface of the inner housing 744 . It may include a second upper skin member 764 provided to be in contact with 762 , and a third upper skin member 766 provided between the first upper skin member 762 and the second upper skin member 764 .
  • the first upper skin material 762 may have a plurality of pores formed so that the cancer cells C can move toward the imitation vascular unit 600, and form one surface of the inner housing 744, there may be
  • the second upper skin member 764 may have a plurality of pores formed similarly to the above-described first upper skin member 762 , and the periphery may be provided so as to be in contact with the first upper skin member 762 .
  • the third epidermis 766 is disposed between the first epidermis 762 and the second epidermis 764 so that the cancer cells C can move toward the imitation vascular unit 600 . It may be composed of a imitation coating layer (F1) that secretes an inducer, and a matrix layer (F2) that prevents the imitation coating layer (F1) from being damaged by the second epidermal material (764).
  • the imitation capsular layer (F1) may be composed of vascular endothelial cells, and the cancer cells (C) form the imitation vascular unit (600) so as to model the departure from the original site in which the cancer cells (C) are first generated.
  • Inducing material is secreted to move toward, and when the cancer cells C floating in the inner housing 644 are moved to an adjacent position, it can be induced to move toward the pseudovascular unit 600 by the inducing material.
  • the substrate layer (F2) comprises the imitation coating layer (F1) and the imitation coating layer (F1) to prevent the imitation coating layer (F1) from being damaged by the pressure of the first and second upper skin members (762) and the second upper layer (764). It may be provided between the two upper skin materials 764 , and a plurality of pores may be formed so that the cancer cells C can move toward the imitation vascular unit 600 , and are formed of a hydrogel to form a hydrogel inside the human body. It is also possible to create an environment similar to the extracellular matrix provided between blood vessels.
  • the stromal layer (F2) prevents damage to the imitation coating layer (F1), forms a plurality of pores through which the cancer cells (C) can move, and makes the movement of the cancer cells (C) more similar to that of the human body. It is possible to create an environment similar to the extracellular matrix in order to create
  • the receiving unit 800 is configured to accommodate the cancer cells (C) when the cancer cells (C) flow out through the spaced gap (622) formed in the imitation blood vessel unit (600).
  • the imitation blood vessel unit 600 is located therein, and the circulation unit 720 may be formed to pass through so that the primary unit 700 can communicate with the inlet (I) and the outlet (O). .
  • an inducer may be accommodated therein to promote the movement of the cancer cells (C) flowing out into the receiving unit 800 through the imitation blood vessel unit 600 .
  • the extraction device 900 can be extracted while moving by the inducing material accommodated in the receiving unit 800, the cancer cells (C) leaked from the imitation blood vessel unit 600, as shown in FIG.
  • An inner space may be formed so that the inducing material passes therethrough and a sedimentation area 940 in which the cancer cells C are precipitated may be divided.
  • the moving region 920 may be in communication with the receiving unit 800 through the circulation device 1000, and the cancer cells ( C) may be a region where the flow rate is slowed by the flow of the inducer.
  • the cancer cells C moved through the circulation device 1000 are relatively heavier than the cancer cells C due to the inducing material whose flow rate is slowed in the movement region 920 . ) is precipitated toward the precipitation region 940 so that the cancer cells C can be extracted.
  • the sedimentation region 940 may have a stepped portion communicating with the moving region 920 , through which the moving region 920 and the sedimentation region 940 may be divided.
  • the circulation device 1000 may be in communication with the extraction device 900 , and may be in communication with the upper portion of the extraction device 900 to communicate with the moving region 920 . there is.
  • the circulation device 1000 may be provided with a pump unit in a portion of the path to provide the power of the inducing material.
  • the cancer cells C accommodated in the inner housing 744 float inside the inner housing 744 by the medium introduced into the inner housing 744 as described above. and the cancer cells C may be moved toward the imitation blood vessel unit 600 by the inducing material of the imitation film layer F1 at a position adjacent to the promotion part 760 .
  • the cancer cell (C) can be moved inside the receiving unit 800 through the spaced gap 622 of the imitation vascular unit 600 as described above, and the inlet (I) and the outlet ( O) may pass through the receiving unit 800 so as to communicate with the primary unit 700 .
  • a imitation film layer (F1) having the same configuration as the imitation film layer (F1) is at the bottom in order to more facilitate the movement of the cancer cells (C) into the accommodation unit (800). It may be provided on the side.
  • the flow rate of the inducing material is slowed by the moving region 920 inside the extraction unit 900, and accordingly, the relatively heavy cancer cells C are precipitated into the precipitation region 940 and the cancer cells. (C) can be extracted.
  • the cancer cells (C) flowing out from the imitation blood vessel unit 600 may flow out through the spaced gap 622 as shown in FIG. 19, and between the ring parts 620, the It can pass through the coating portion (620).
  • the movement can be facilitated by the inducing material secreted by the secretory member 644 or the inducing material accommodated in the receiving unit 800, and the cover member 642, the secretory member ( 644), passing through the pores formed in the moisture retaining member 646, it will be able to move into the receiving unit (800).
  • the moisture retaining member 646 surrounds the secretion member 644 of the spaced gap 622 to retain moisture in the secretion member 644 so that the movement of the cancer cells C is not restricted. may be formed accordingly.
  • the imitation blood vessel unit 600 is connected to the connecting parts 660 facing each other in the spaced gap 622 .
  • a reinforcing part 680 for reinforcing the rigidity of may be formed.
  • the reinforcing part 680 may be formed to cross the spaced gap 622 to connect the connecting parts 660 facing each other, and the ring part so that the spaced gap 622 is not closed. It may be formed to have a radius of curvature along the circumferential curve of 620 .
  • they may be sequentially arranged from the inlet (I) toward the outlet (O) so as to be shifted from each other, may be arranged in the same direction, or may be arranged to cross each other .
  • the spaced gap 622 may be closed to open the spaced gap 622 so that the cancer cells C are not limited to outflow.
  • the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same including the imitation vascular unit according to an embodiment of the present invention, are provided in which the cancer cells (C) deviate from the original site of origin and collect blood vessels. Moving along, passing through the blood vessel wall and moving through the metastasis can be modeled, and through this, a large number of samples can be taken to observe transformation while departing from the blood vessel wall or the original site of origin, and accordingly, when the drug is injected A drug can be injected by targeting the specific cancer cell (C), and the progress can be observed through this.
  • the transformed cancer cell collection device including the imitation vascular unit and the transformation collection and observation system of cancer cells using the same according to an embodiment of the present invention can perform transformation of cancer cells (C) passing through vascular endothelial cells using various samples. There may be effects that can be collected and observed.
  • the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same including the imitation vascular unit monitor the collection state and transformation of cancer cells (C) passing through vascular endothelial cells.
  • a sensor interworking with the manager's terminal may be provided for easy adjustment.
  • the cancer cells (C) can be sensed in order to check the period and amount in which the cancer cells (C) located in the kicking part 740 are moved toward the pseudovascular unit 600 by the flow of the medium.
  • the amount of movement of the cancer cells (C) per time, the movement period, the amount of movement of the cancer cells (C) according to time, etc. of the manager You can check it through the terminal.
  • the cancer cells (C) are sensed so that it can be confirmed that the cancer cells (C) moved from the imitation blood vessel unit (600) to the receiving unit (800) are moved to the extraction device (900) according to the flow of the fluid.
  • a power unit that provides power to circulate the medium so as to control the transformed cancer cell collection device including the imitation blood vessel unit of the present invention and the transformation collection and observation system of cancer cells using the same by using the manager's terminal It is also possible to control the intensity and flow rate of the flow of the medium through the manager's terminal in conjunction with the pump unit to control the flow rate and flow of the inducing material so that the cancer cells (C) are inside the extraction device 1000 It can be controlled so that it is effectively precipitated into the precipitation region 940 in the .
  • a management server for managing data measured through the first sensor and the second sensor receives the data measured through the first sensor and the second sensor to determine the time, the power unit and It is possible to analyze the amount of movement of the cancer cells (C) according to the strength of the pump unit and the amount of cancer cells (C) accommodated inside the single primary unit 700, and it is interlocked with the power unit and the pump unit to supply a medium or an inducing material. It may be provided to control the supply unit to supply.
  • the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same include the flow of the medium or the inducing material through the terminal, the cancer cells (C ) can be determined and managed by measuring the amount of movement.

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Abstract

A cancer cell transformation observation system using a circulation-type cancer cell transformation measuring device and a device for collecting transformed cancer cells comprising an artificial blood vessel unit according to the present invention comprises a measurement unit for inducing the transformation of cancer cells in order to observe the transformation of the cancer cells that have passed through the endothelium, the measurement unit comprising: a source unit which is divided into an outer case and an inner case in which the cancer cells are accommodated; a supply unit communicating with the outer case to provide a flow path for a medium to pass through the source unit; a moving unit coupled to one surface of the inner case to provide a flow path through which the cancer cells are moved; and an induction unit provided between the inner case and the moving unit and secreting an inducer so that the cancer cells floating inside the inner case can be moved toward the moving unit by the flow of the medium.

Description

순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템Cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a imitation blood vessel unit
본 발명은 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템에 관한 것으로, 보다 상세하게는 암세포가 혈관벽을 통과하며 형질전환되는 현상을 관찰하기 위한 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템에 관한 것이다.The present invention relates to a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same, and more particularly, observing a phenomenon in which cancer cells are transformed as they pass through a blood vessel wall. It relates to a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells, and a cancer cell transformation observation system using the same.
또한, 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 관찰 시스템에 관한 것으로, 보다 상세하게는 혈관내피세포를 통과하는 암세포의 형질전환을 다양한 샘플을 채취하여 관찰할 수 있는 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 관찰 시스템에 관한 것이다.In addition, it relates to a transformed cancer cell collection device comprising a pseudovascular unit and a transformation observation system for cancer cells using the same, and more particularly, it is possible to observe transformation of cancer cells passing through vascular endothelial cells by collecting various samples. It relates to a device for collecting transformed cancer cells including a mimetic vascular unit and a system for observing transformation of cancer cells using the same.
암세포는 최초 발생한 원발지에서 벗어나 혈관을 타고 이동되며 또 다른 장기에서 콜로니화되어 또 다른 장기에 전이될 수 있다.Cancer cells move through blood vessels away from the original site of origin, colonize in another organ, and metastasize to another organ.
이와 같이 원발지에서 벗어난 암세포는 다른 장기로 혈관을 타고 이동되어 혈관벽을 통과해 전이부로 이동될 수 있다.As such, cancer cells that have escaped from the original site can move through blood vessels to other organs, pass through the blood vessel wall, and move to metastases.
다만, 이와 같이 암세포가 혈관벽을 통과하여 전이부로 이동된 암세포는 최초 발생한 원발지의 암세포와 유사한 형질을 가지고 있을 수 있으나, 일부 암세포는 형질전환되어 최초 발생한 원발지의 암세포와 다른 형태의 암세포로 전환되어 서로 다른 치료제를 사용해야 하는 상황이 발생될 수 있다.However, the cancer cells in which the cancer cells have passed through the blood vessel wall and moved to the metastasis may have similar traits to the cancer cells of the original origin, but some cancer cells are transformed and converted into cancer cells of a different type from the cancer cells of the original origin. Circumstances may arise that require the use of other therapeutic agents.
이와 같이 혈관벽을 통과하거나 혈관을 따라 이동되는 암세포에 대하여 형질전환된 암세포의 다양한 샘플을 수집하기 위한 다양한 시도가 이루어지고 있으나, 암세포가 혈관내피세포층을 통과하는 모델링이 인체의 환경과 유사하도록 조성하는 것은 사실상 어렵기 때문에 최초 발생한 원발지와 전이부의 암세포의 샘플을 채취하는 것에는 다소 어려움이 있을 수 있다.As described above, various attempts have been made to collect various samples of transformed cancer cells for cancer cells that pass through the blood vessel wall or move along the blood vessel. Since it is difficult in reality, it may be somewhat difficult to collect samples of cancer cells from the first origin and metastases.
이에 따라 암세포가 혈관벽을 통과하며 형질전환되는 현상을 관찰할 수 있는 수단이 활발하게 고안되고 있으며, 이를 해결할 수 있는 수단이 필요하다.Accordingly, a means for observing the transformation of cancer cells through the blood vessel wall is being actively devised, and a means for solving this is required.
본 발명은 상기와 같은 문제점을 해결하기 위한 것으로서, 혈관내피세포를 통과하는 암세포의 형질전환을 다양한 샘플을 채취하여 관찰할 수 있는 것을 과제로 한다.The present invention is intended to solve the above problems, and an object of the present invention is to be able to observe transformation of cancer cells passing through vascular endothelial cells by collecting various samples.
본 발명의 과제들은 이상에서 언급한 과제들로 제한되지 않으며, 언급되지 않는 또 다른 과제들은 아래의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.
상기한 과제를 해결하기 위하여, 본 발명의 형태에 따르면, 내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서, 외통과 상기 암세포가 수용되는 내통으로 구분되는 진원부, 상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부, 상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부 및 상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 진원부 내부를 부유하는 상기 암세포가 상기 이동부를 향해 이동되도록 유도물질을 분비하는 유도부를 포함한다.In order to solve the above problems, according to an aspect of the present invention, as a measurement unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium, the epicenter is divided into an outer tube and an inner tube in which the cancer cells are accommodated. part, a supply part communicating with the outer cylinder to provide a flow path for the medium to pass through the round part, a moving part coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells move, and the inner cylinder and the moving part provided between the moving part. and an induction part that secretes an inducer so that the cancer cells floating inside the round part move toward the moving part by the flow of the medium.
여기서 상기 유도부는, 이탈된 상기 암세포가 상기 이동부로 이동되도록 상기 암세포가 통과할 수 있는 다수의 막으로 형성되는 것을 특징으로 한다.Here, the induction part is characterized in that it is formed of a plurality of membranes through which the cancer cells can pass so that the separated cancer cells are moved to the moving part.
아울러 상기 유도부는, 상기 진원부의 일면에 형성되는 제1 피막부재, 상기 제1 피막부재와 접촉되도록 구비되는 제2 피막부재 및 상기 제1 피막부재와 상기 제2 피막부재 사이에 구비되어 상기 암세포가 상기 이동부로 이동되도록 유도하며, 인체 내부의 혈관과 유사한 내피층을 형성하는 모조혈관내피부재를 포함하는 것을 특징으로 한다.In addition, the induction part may include a first encapsulation member formed on one surface of the round part, a second encapsulation member provided to be in contact with the first encapsulation member, and a space provided between the first encapsulation member and the second encapsulation member so that the cancer cells are It guides to move to the moving part, characterized in that it comprises a imitation blood vessel inner skin material that forms an endothelial layer similar to blood vessels inside the human body.
이때, 상기 모조혈관내피부재는, 상기 암세포가 통과할 수 있도록 혈관 내피세포로 형성되어 상기 제1 피막부재에 부착되고, 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 혈관내피층 및 상기 혈관내피층과 상기 제2 피막부재 사이에 구비되어 상기 혈관내피층이 상기 제2 피막부재의 압력에 의해 손상되는 것을 방지하는 히드로겔층을 포함하는 것을 특징으로 한다.At this time, the imitation vascular endothelial material is formed of vascular endothelial cells so that the cancer cells can pass through, is attached to the first encapsulation member, and secretes an inducer to move the cancer cells to the moving part, and the vascular endothelium and the vascular endothelium. It characterized in that it comprises a hydrogel layer provided between the layer and the second encapsulation member to prevent the vascular endothelial layer from being damaged by the pressure of the second encapsulation member.
한편, 상기 진원부는, 상기 외통과 상기 내통이 소정거리 이격되도록 배치되는 것을 특징으로 한다.On the other hand, the circular part, characterized in that the outer cylinder and the inner cylinder are arranged to be spaced apart a predetermined distance.
여기서 상기 내통은, 상기 공급부를 통해 상기 외통으로 유입되는 상기 배지가 내부로 유입되도록 상부를 향해 개구부가 형성되는 것을 특징으로 한다.Here, the inner cylinder is characterized in that the opening is formed toward the upper side so that the medium flowing into the outer cylinder through the supply unit is introduced into the inside.
아울러 상기 내통은, 상기 배지는 유입되고 상기 암세포는 상기 개구부로 이탈되는 것을 방지하도록 상기 개구부에 거름부재가 구비되는 것을 특징으로 한다.In addition, the inner cylinder is characterized in that the medium is introduced and a filtering member is provided in the opening to prevent the cancer cells from leaving the opening.
한편, 상기 이동부는, 상기 암세포가 상기 이동부로 이동될 수 있도록 상기 유도부와 상기 외통 사이에서 상기 암세포의 이동을 가이드하는 가이드부재를 포함하는 것을 특징으로 한다.Meanwhile, the moving unit may include a guide member for guiding the movement of the cancer cells between the guide unit and the outer cylinder so that the cancer cells can be moved to the moving unit.
여기서 상기 가이드부재는, 상기 유도부의 둘레에서 상기 이동부를 향해 직경이 좁아지도록 연장되는 것을 특징으로 한다.Here, the guide member is characterized in that it extends from the periphery of the guide part toward the moving part so that the diameter becomes narrower.
상술한 암세포의 형질전환을 유도하는 측정유닛을 바탕으로 이를 포함하는 순환식 암세포 형질전환 측정장치는, 내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서, 외통과 암세포가 수용되는 내통으로 구분되는 진원부, 상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부, 상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부 및 상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 진원부 내부를 부유하는 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 유도부를 포함하는 암세포의 형질전환을 유도하는 측정유닛 및 상기 이동부와 연통되고, 상기 이동부를 따라 이동되는 상기 암세포 및 상기 배지가 상기 진원부로 순환되도록 유로를 제공하는 순환유닛를 포함한다.Based on the above-described measuring unit for inducing transformation of cancer cells, the circulating cancer cell transformation measuring device including the same is a measuring unit for inducing transformation of cancer cells to observe the transformation of cancer cells that have passed through the endothelium. A round part divided into an inner cylinder in which the cancer cells are accommodated, a supply part communicating with the outer cylinder to provide a flow path for the medium to pass through the round part, a moving part coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells are moved, and the A measuring unit for inducing transformation of cancer cells, which is provided between the inner passage and the moving unit and includes an inducing unit that secretes an inducer so that the cancer cells floating inside the round part are moved to the moving unit by the flow of the medium; and and a circulation unit communicating with the moving unit and providing a flow path for circulating the cancer cells and the medium moving along the moving unit to the circular unit.
여기서 상기 순환유닛은, 유로상에 구비되어 상기 이동부를 통해 이동되는 상기 암세포를 수집하는 수집부를 포함하는 것을 특징으로 한다.Here, the circulation unit is provided on the flow path and characterized in that it includes a collecting unit for collecting the cancer cells moving through the moving unit.
이 때, 상기 수집부는, 유로 일부를 대체하며, 상기 암세포가 추출되도록 다수의 막으로 형성되는 추출부재 및 상기 추출부재 사이에 구비되어 상기 암세포의 이동을 촉진시키는 유도물질을 분비하고, 상기 암세포가 상기 추출부재를 통해 외부로 추출되는 것을 촉진시키는 촉진부재를 포함하는 것을 특징으로 한다.At this time, the collection unit replaces a part of the flow path, and is provided between the extraction member and the extraction member formed of a plurality of membranes so that the cancer cells are extracted, and secretes an inducer to promote the movement of the cancer cells, and the cancer cells are It is characterized in that it comprises a facilitating member for facilitating extraction to the outside through the extraction member.
아울러 상기 순환유닛은, 상기 배지 및 상기 암세포의 흐름이 적어도 하나 이상의 유로로 분할되는 서클부를 포함하는 것을 특징으로 한다.In addition, the circulation unit, characterized in that it comprises a circle portion in which the flow of the medium and the cancer cells is divided into at least one or more flow passages.
여기서 상기 서클부는, 곡률반경을 가지며 절곡되어 분할된 유로가 통합되는 폐루프가 형성되는 것을 특징으로 한다.Here, the circle portion has a radius of curvature and is bent to form a closed loop in which the divided flow paths are integrated.
상술한 순환식 암세포 형질전환 측정장치를 이용한 암세포 형질전환 관찰 시스템은, 내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서, 외통과 암세포가 수용되는 내통으로 구분되는 진원부, 상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부, 상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부 및 상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 진원부 내부를 부유하는 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 유도부를 포함하는 암세포의 형질전환을 유도하는 복수의 측정유닛, 상기 복수의 측정유닛과 연통되도록 유로가 분기되는 복수의 분기점이 형성되고, 상기 복수의 분기점이 서로 이어지도록 유로가 형성되어 상기 암세포 및 상기 배지가 순환되는 순환유닛을 포함하고, 상기 복수의 측정유닛은, 상기 이동부가 상기 순환유닛을 향하도록 방사형으로 배치되는 것을 특징으로 한다.The cancer cell transformation observation system using the above-described circulating cancer cell transformation measuring device is a measurement unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium, and is divided into an outer passage and an inner tube in which cancer cells are accommodated. a circular part to be in communication with the outer cylinder to provide a flow path for the medium to pass through the circular part, a moving part coupled to one surface of the inner cylinder to provide a flow path for the cancer cells to move, and provided between the inner cylinder and the moving part a plurality of measurement units for inducing transformation of cancer cells and communicating with the plurality of measurement units, which includes an induction unit that secretes an inducer so that the cancer cells floating inside the round cell are moved to the moving unit by the flow of the medium A plurality of branch points at which the flow passages branch are formed so as to be possible, and the flow passages are formed so that the plurality of branch points are connected to each other so as to include a circulation unit in which the cancer cells and the medium are circulated, wherein the plurality of measurement units include: It is characterized in that it is radially arranged to face the unit.
여기서 상기 순환유닛은, 유로상에 구비되어 상기 이동부를 통해 이동되는 상기 암세포를 수집하는 수집부를 포함하는 것을 특징으로 한다.Here, the circulation unit is provided on the flow path and characterized in that it includes a collecting unit for collecting the cancer cells moving through the moving unit.
이 때, 상기 수집부는, 유로의 일부를 대체하며, 상기 암세포가 추출되도록 다수의 막으로 형성되는 추출부재 및 상기 추출부재 사이에 구비되어 상기 암세포의 이동을 촉진시키는 유도물질을 배출하고, 상기 암세포가 상기 추출부재를 통해 외부로 추출되는 것을 촉진시키는 촉진부재를 포함하는 것을 특징으로 한다.At this time, the collection unit replaces a part of the flow path, and is provided between the extraction member and the extraction member formed of a plurality of membranes so that the cancer cells are extracted, and discharges an inducing material that promotes the movement of the cancer cells, and the cancer cells It is characterized in that it comprises a facilitating member that promotes extraction to the outside through the extraction member.
한편, 상기 복수의 측정유닛은, 상기 유도부에서 분비하는 유도물질 및 상기 암세포가 상기 순환유닛을 통해 서로 상호 교환되는 것을 특징으로 한다.Meanwhile, in the plurality of measurement units, the inducing material secreted by the induction unit and the cancer cells are exchanged with each other through the circulation unit.
이 때, 상기 순환유닛은, 일측에서 타측을 향해 곡률을 가지며 구부러져 일측과 타측이 서로 이어지는 폐루프를 가지는 서클부를 포함하는 것을 특징으로 한다.In this case, the circulation unit is characterized in that it includes a circle portion having a closed loop having a curvature from one side toward the other side and being bent so that one side and the other side are connected to each other.
한편, 주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서, 경로상에 복수개가 이격되도록 경로를 따라 배치되어 상기 암세포가 외부로 유출되도록 이격된 틈을 형성하는 고리부, 내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 이격된 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 되는 피막부를 포함한다.On the other hand, an inlet and outlet are formed, and in order to observe transformation of cancer cells passing through the blood vessel wall, a pseudovascular unit is formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet. A ring portion disposed along a path to be spaced apart to form a gap spaced apart so that the cancer cells are discharged to the outside, a plurality of the ring portions are positioned therein, and a plurality of membranes are formed so that the cancer cells flow out from the spaced gap It secretes an inducer as much as possible, and includes a membrane portion serving as a path through which the medium and the cancer cells move.
아울러 상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 한다.In addition, a portion of the periphery of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
여기서 상기 연결부는, 상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 한다.Here, the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
또한, 상기 연결부는, 상기 이격된 틈에서 인접한 상기 고리부를 연결하며 복수의 상기 고리부의 간격을 유지시키는 것을 특징으로 한다.In addition, the connecting portion, connecting the ring portion adjacent in the spaced gap, characterized in that it maintains the spacing of the plurality of the ring portion.
이 때, 상기 연결부는, 상기 고리부의 둘레를 따라 다수개가 형성되는 것을 특징으로 한다.At this time, the connecting portion is characterized in that a plurality is formed along the circumference of the ring portion.
한편, 상기 피막부는, 상기 고리부 및 상기 연결부를 감싸도록 구비되는 덮개부재, 상기 덮개부재의 둘레를 따라 형성되고, 상기 암세포가 외부로 이동되도록 유도물질을 분비하는 분비부재를 포함하는 것을 특징으로 한다.On the other hand, the film portion, the cover member provided to surround the ring portion and the connection portion, is formed along the circumference of the cover member, characterized in that it comprises a secretion member that secretes an inducer to move the cancer cells to the outside. do.
여기서 상기 분비부재는, 혈관내피세포로 구성되는 것을 특징으로 한다.Here, the secretory member is characterized in that it is composed of vascular endothelial cells.
아울러 상기 피막부는, 상기 분비부재의 수분이 유지되도록 상기 분비부재의 둘레를 따라 구비되는 수분유지부재를 더 포함하는 것을 특징으로 한다.In addition, the film portion, characterized in that it further comprises a moisture retention member provided along the circumference of the secretion member so that the moisture of the secretion member is maintained.
여기서 상기 수분유지부재는, 상기 고리부 사이에서 상기 분비부재의 둘레를 따라 형성되어 상기 분비부재를 감싸도록 형성되는 것을 특징으로 한다.Here, the moisture retention member is formed along the circumference of the secretion member between the ring parts, characterized in that it is formed to surround the secretion member.
한편, 상기 고리부는, 상기 피막부의 내측 둘레와 접하며 상기 피막부를 지지하는 것을 특징으로 한다.On the other hand, the ring portion, in contact with the inner periphery of the film portion, characterized in that for supporting the film portion.
상술한 구성을 포함하는 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치는, 주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서, 경로상에 복수개가 이격되도록 배치되어 상기 암세포가 외부로 유출되도록 틈을 형성하는 고리부, 내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 형성되는 피막부를 포함하는 모조혈관유닛, 내부에 공간이 형성되어 상기 암세포가 위치되고, 상기 주입구 및 상기 배출구와 연통되어 상기 배지가 순환되는 원발유닛 및 상기 모조혈관유닛이 내부를 관통하며 배치되고, 상기 모조혈관유닛에서 외부로 이동되는 상기 암세포를 수집하는 수용유닛을 포함한다.In the transformed cancer cell collecting device including the pseudovascular unit according to an embodiment of the present invention including the above-described configuration, an inlet and an outlet are formed, and in the inlet to observe transformation of cancer cells passing through the blood vessel wall, A imitation vascular unit formed in a portion of a path through which the medium and cancer cells move toward the outlet, a ring portion disposed to be spaced apart on the path to form a gap so that the cancer cells are discharged to the outside, a plurality of the rings inside An imitation vascular unit having an additional location, formed of a plurality of membranes, secreting an inducer to allow the cancer cells to flow out from the gap, and including a membrane portion in which the medium and a path through which the cancer cells move are formed, a space inside Formed, the cancer cells are located, and the primary unit and the imitation vascular unit are disposed in communication with the inlet and the outlet to circulate the medium, and collect the cancer cells moving from the imitation vascular unit to the outside Includes a receiving unit.
여기서 상기 수용유닛은, 상기 모조혈관유닛을 통해 이동하는 상기 암세포가 외부로 이동되는 것을 촉진하도록 유도물질이 수용되는 것을 특징으로 한다.Here, the receiving unit is characterized in that the inducer is accommodated to promote the movement of the cancer cells moving through the pseudovascular unit to the outside.
한편, 상기 원발유닛은, 상기 주입구 및 상기 배출구와 연결되어 배지가 순환되는 순환부, 상기 순환부와 연통되는 외부 하우징과 상기 외부 하우징과 이격되어 내부에 암세포가 위치되고, 상부를 향해 개구부가 형성되는 내부 하우징으로 구분되는 발상부, 상기 배지의 순환에 의해 상기 내부 하우징을 부유하는 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 유도물질을 분비하며 상기 내부 하우징의 일면에 다수의 막으로 형성되는 촉진부를 포함하는 것을 특징으로 한다.On the other hand, in the primary unit, a circulation part connected to the inlet and the outlet to circulate the medium, an external housing communicating with the circulation part and spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top The propulsion part is divided into an inner housing that becomes an inner housing, which secretes an inducing material so that the cancer cells floating in the inner housing move toward the pseudovascular unit by the circulation of the medium, and promotes formation of a plurality of membranes on one surface of the inner housing It is characterized by including wealth.
한편, 상기 촉진부는, 상기 발상부의 일면에 형성되는 제1 상피부재, 상기 제1 막과 접촉되도록 구비되는 제2 상피부재, 상기 제1 막 및 상기 제2 막 사이에 구비되어 유도물질을 분비하는 제3 상피부재를 포함하는 것을 특징으로 한다.On the other hand, the facilitating part is provided between the first upper skin material formed on one surface of the propulsion part, the second upper skin material provided to be in contact with the first film, and the first film and the second film to secrete an inducer. It is characterized in that it comprises a third upper skin material.
여기서 상기 제3 상피부재는, 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 혈관내피세포로 구비되어 제1 상피부재에 부착되는 모조피막층 및 상기 제1 상피부재 및 상기 제2 상피부재에 의해 상기 모조피막층이 손상되는 것을 방지하기 위해 상기 모조피막층과 상기 제2 상피부재 사이에 구비되는 기질층을 포함하는 것을 특징으로 한다.Here, the third epidermal material is provided with vascular endothelial cells so that the cancer cells are moved toward the imitation vascular unit, and the imitation film layer is attached to the first epidermal material, and the imitation by the first epidermis and the second epidermis. It characterized in that it comprises a substrate layer provided between the imitation film layer and the second upper skin material in order to prevent the film layer from being damaged.
한편, 상기 내부 하우징은, 상기 암세포가 외부로 이탈되는 것을 방지하기 위해 상기 개구부에 구비되는 거름부재를 포함하는 것을 특징으로 한다.On the other hand, the inner housing, characterized in that it includes a filtering member provided in the opening in order to prevent the cancer cells from escaping to the outside.
한편, 상기 모조혈관유닛은, 상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 한다.On the other hand, the imitation blood vessel unit, a portion of the circumference of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
여기서 상기 연결부는, 상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 한다.Here, the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
상술한 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 본 발명의 일 실시예에 따른 암세포의 형질전환 관찰 시스템은, 주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서, 경로상에 복수개가 이격되도록 배치되어 상기 암세포가 외부로 유출되도록 틈을 형성하는 고리부, 내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 형성되는 피막부를 포함하는 모조혈관유닛, 내부에 공간이 형성되어 상기 암세포가 위치되고, 상기 주입구 및 상기 배출구와 연통되어 상기 배지가 순환되는 원발유닛 및 상기 모조혈관유닛이 내부를 관통하며 배치되고, 상기 모조혈관유닛에서 외부로 이동되는 상기 암세포를 수집하는 수용유닛을 포함하는 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치, 유도물질이 상기 수용유닛을 통과하여 순환되도록 상기 수용유닛과 연통되는 순환장치 및 상기 순환유닛의 경로상에 형성되며, 상기 수용유닛으로 이동된 상기 암세포가 유도물질의 흐름에 따라 이동되어 수집되는 추출장치를 포함한다.The system for observing transformation of cancer cells according to an embodiment of the present invention using the transformed cancer cell collection device including the above-described pseudovascular unit, an inlet and an outlet are formed, and observe the transformation of cancer cells passing through the blood vessel wall. As a imitation vascular unit formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet, a plurality of vascular units are arranged to be spaced apart on the path to form a gap so that the cancer cells are discharged to the outside, inside A imitation vascular unit comprising a membrane portion in which a plurality of the ring portions are located, a plurality of membranes are formed to secrete an inducer to allow the cancer cells to flow out from the gap, and a membrane portion in which the medium and a path through which the cancer cells move are formed; A space is formed inside, the cancer cells are located, and the primary unit and the imitation vascular unit in which the medium is circulated in communication with the inlet and the outlet are disposed while penetrating the inside, and the imitation vascular unit is moved to the outside A transformed cancer cell collecting device including a dummy vascular unit including a receiving unit for collecting cancer cells, a circulator communicating with the receiving unit so that an inducer is circulated through the receiving unit, and formed on the path of the circulation unit and an extraction device in which the cancer cells moved to the receiving unit are moved and collected according to the flow of the inducer.
여기서 상기 추출장치는, 내부 공간이 단턱지게 형성되어 상기 암세포가 내부 공간에 침전되도록 하부에 공간이 형성되는 것을 특징으로 한다.Here, the extraction device is characterized in that the inner space is formed to be stepped, and a space is formed in the lower part so that the cancer cells are precipitated in the inner space.
아울러 상기 순환장치는, 유도물질의 이동경로상에 상기 유도물질이 이동되도록 동력을 제공하는 펌프부를 포함하는 것을 특징으로 한다.In addition, the circulation device is characterized in that it includes a pump unit for providing power to move the inducer on the movement path of the inducer.
한편, 상기 원발장치는, 상기 주입구 및 상기 배출구와 연결되어 배지가 순환되는 순환부, 상기 순환부와 연통되는 외부 하우징과 상기 외부 하우징과 이격되어 내부에 암세포가 위치되고, 상부를 향해 개구부가 형성되는 내부 하우징으로 구분되는 발상부, 상기 배지의 순환에 의해 상기 내부 하우징을 부유하는 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 유도물질을 분비하며 상기 내부 하우징의 일면에 다수의 막으로 형성되는 촉진부를 포함하는 것을 특징으로 한다.On the other hand, in the primary device, a circulation part connected to the inlet and the outlet to circulate the medium, an external housing communicating with the circulation part and spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top The propulsion part is divided into an inner housing that becomes an inner housing, which secretes an inducing material so that the cancer cells floating in the inner housing move toward the pseudovascular unit by the circulation of the medium, and promotes formation of a plurality of membranes on one surface of the inner housing It is characterized by including wealth.
여기서 상기 촉진부는, 상기 발상부의 일면에 형성되는 제1 상피부재, 상기 제1 막과 접촉되도록 구비되는 제2 상피부재, 상기 제1 막 및 상기 제2 막 사이에 구비되어 유도물질을 분비하는 제3 상피부재를 포함하는 것을 특징으로 한다.Here, the facilitating unit may include a first upper skin material formed on one surface of the propulsion part, a second upper skin material provided to be in contact with the first film, and a second upper skin material provided between the first film and the second film to secrete an inducer. 3 It is characterized in that it includes an upper skin material.
또한, 상기 제3 상피부재는, 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 혈관내피세포로 구비되어 제1 상피부재에 부착되는 모조피막층 및 상기 제1 상피부재 및 상기 제2 상피부재에 의해 상기 모조피막층이 손상되는 것을 방지하기 위해 상기 모조피막층과 상기 제2 상피부재 사이에 구비되는 기질층을 포함하는 것을 특징으로 한다.In addition, the third epidermal material is provided with vascular endothelial cells so that the cancer cells are moved toward the imitation vascular unit, and the imitation coating layer is attached to the first epidermis, and the first epidermis and the second epidermis. In order to prevent the imitation coating layer from being damaged, it characterized in that it comprises a substrate layer provided between the imitation coating layer and the second upper skin material.
한편, 상기 모조혈관유닛은, 상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 한다.On the other hand, the imitation blood vessel unit, a portion of the circumference of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other.
여기서 상기 연결부는, 상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 한다.Here, the connecting portion is formed to be elongated from the injection port toward the outlet so that the ring portion is fixed on the path to connect the plurality of ring portions from the inside of the coating portion.
본 발명의 순환식 암세포 형질전환 측정장치 및 모조혈관을 유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템에 따르면, 혈관내피세포를 통과하는 암세포의 형질전환을 다양한 샘플을 채취하여 관찰할 수 있는 효과가 있다.According to the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit of the present invention, the transformation of cancer cells passing through vascular endothelial cells can be performed by collecting various samples. There is an observable effect.
본 발명의 효과들은 이상에서 언급한 효과들로 제한되지 않으며, 언급되지 않은 또 다른 효과들은 청구범위의 기재로부터 당업자에게 명확하게 이해될 수 있을 것이다.Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.
아래에서 설명하는 본 출원의 바람직한 실시예의 상세한 설명뿐만 아니라 위에서 설명한 요약은 첨부된 도면과 관련해서 읽을 때에 더 잘 이해될 수 있을 것이다.The summary set forth above as well as the detailed description of the preferred embodiments of the present application set forth below may be better understood when read in conjunction with the accompanying drawings.
본 발명을 예시하기 위한 목적으로 도면에는 바람직한 실시예들이 도시되어 있다.For the purpose of illustrating the invention, there are shown in the drawings preferred embodiments.
그러나, 본 출원은 도시된 정확한 배치와 수단에 한정되는 것이 아님을 이해해야 한다.It should be understood, however, that the present application is not limited to the precise arrangements and instrumentalities shown.
도 1은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 측정유닛의 전체적인 모습을 도시한 도면;1 is a view showing the overall appearance of a measuring unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 2는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 유도부를 설명하기 위해 도시한 도면;2 is a view illustrating an induction unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 3은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 배지 및 암세포의 흐름을 설명하기 위해 도시한 도면;3 is a diagram illustrating the flow of the medium and cancer cells in the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention. drawing;
도 4는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 측정장치를 설명하기 위해 도시한 도면;4 is a view for explaining a measuring device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 5는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 수집부를 설명하기 위해 도시한 도면;5 is a view illustrating a collection unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 6은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 순환유닛을 설명하기 위해 도시한 도면;6 is a view for explaining the circulation unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention;
도 7은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 측정유닛 내부의 상황을 설명하기 위해 도시한 도면;7 is a diagram illustrating the situation inside the measuring unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention. drawing;
도 8은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 복수의 측정유닛이 구비되는 상황을 설명하기 위해 도시한 도면;8 illustrates a situation in which a plurality of measuring units are provided in a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention. drawings for;
도 9는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 상호관계를 설명하기 위해 도시한 도면;FIG. 9 is a diagram illustrating the relationship between a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 10은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 관찰 시스템을 설명하기 위해 도시한 도면;10 is a diagram illustrating an observation system of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 11은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 포함하는 전반적인 모습을 도시한 도면;11 is an overall view of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit, according to an embodiment of the present invention, including a dummy vascular unit. drawing;
도 12는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 설명하기 위해 도시한 도면;12 is a view for explaining the imitation vascular unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention;
도 13은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 피막부를 설명하기 위해 도시한 도면;13 is a view for explaining the capsule part of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention;
도 14는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 원발유닛을 설명하기 위해 도시한 도면;14 is a view illustrating a primary unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 15는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 촉진부를 설명하기 위해 도시한 도면;15 is a view for explaining a facilitating unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 16은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 추출장치를 설명하기 위해 도시한 도면;16 is a view for explaining an extraction device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention;
도 17은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 포함하는 상태에서 전체적인 흐름을 설명하기 위해 도시한 도면;17 is a view showing the overall flow of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit according to an embodiment of the present invention, in a state including the imitation vascular unit; drawings for the purpose of explanation;
도 18은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 통해 암세포가 유출되는 상황을 설명하기 위해 도시한 도면;18 is a diagram illustrating a situation in which cancer cells flow out through a dummy vascular unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention. drawings for the purpose of explanation;
도 19는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 암세포가 모조혈관유닛에 빠져나가는 것을 보다 상세하게 설명하기 위해 도시한 도면; 및19 is a more detailed view of cancer cells in the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention A drawing shown to explain; and
도 20은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛에 보강부가 형성되는 것을 도시한 도면이다.20 is a view showing that a reinforcement part is formed in the imitation blood vessel unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention; It is a drawing.
*<부호의 설명>*<Explanation of code>
100: 진원부100: epicenter
200: 공급부200: supply
300: 유도부300: induction part
400: 이동부400: moving unit
500: 순환유닛500: circulation unit
600: 모조혈관유닛600: imitation vascular unit
700: 원발유닛700: primary unit
800: 수용유닛800: accommodation unit
900: 추출장치900: extractor
1000: 순환장치1000: circulation device
이하 본 발명의 목적이 구체적으로 실현될 수 있는 본 발명의 바람직한 실시예를 첨부된 도면을 참조하여 설명한다.Hereinafter, preferred embodiments of the present invention in which the object of the present invention can be specifically realized will be described with reference to the accompanying drawings.
본 실시예를 설명함에 있어서, 동일 구성에 대해서는 동일 명칭 및 동일 부호가 사용되며 이에 따른 부가적인 설명은 생략하기로 한다.In describing the present embodiment, the same names and the same reference numerals are used for the same components, and an additional description thereof will be omitted.
먼저 도 1 내지 도 2를 참조하여 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛의 구성에 대해 설명할 수 있다.First, the configuration of the measurement unit for inducing transformation of cancer cells according to an embodiment of the present invention may be described with reference to FIGS. 1 and 2 .
구체적으로 도 1은 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 측정유닛의 전체적인 모습을 도시한 도면, 도 2는 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 유도부를 설명하기 위해 도시한 도면이다.Specifically, FIG. 1 is a view showing the overall appearance of a measuring unit of a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention , FIG. 2 is a diagram illustrating a circulating cancer cell transformation measuring apparatus including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an induction unit of a cancer cell transformation observation system using the same.
여기서 도 1에 도시된 바와 같이 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛은 진원부(100), 배지가 진원부(100)를 통과하도록 유로를 제공하는 공급부(200), 암세포(C)가 이동부(400)를 향해 이동되도록 유도물질을 분비하는 유도부(300) 및 암세포(C)가 이동되는 유로를 제공하는 이동부(400)로 구성될 수 있다.Here, as shown in FIG. 1 , the measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention is the epicenter 100 , and the supply unit 200 providing a flow path for the medium to pass through the epicenter 100 . , it may be composed of an induction unit 300 that secretes an inducer so that the cancer cells C move toward the moving unit 400 and a moving unit 400 that provides a flow path through which the cancer cells C move.
보다 상세하게 상기 진원부(100)는 외통(120)과 상기 외통(120)과 구분되도록 배치되며, 상기 외통(120)과 소정거리 이격되어 상기 암세포(C)가 수용되는 내통(140)으로 구성될 수 있다.In more detail, the round part 100 is arranged to be separated from the outer cylinder 120 and the outer cylinder 120, and is spaced apart from the outer cylinder 120 by a predetermined distance and consists of an inner cylinder 140 in which the cancer cells C are accommodated. can be
여기서 상기 내통(140)은 상기 배지가 유입될 수 있도록 상부를 향해 개구부가 형성될 수 있으며, 상기 개구부를 통해 유입되는 상기 배지의 흐름에 의해 상기 내통(140)을 부유하는 상기 암세포(C)가 상기 개구부를 통해 이탈되는 것을 방지하기 위해 상기 배지는 자유롭게 상기 개구부를 이동할 수 있지만, 상기 암세포(C)는 상기 내통(140)에 고립되도록 상기 개구부에는 상기 암세포(C)의 이탈을 제한하는 거름부재(142)가 형성될 수 있다.Here, the inner tube 140 may have an opening upward so that the medium can be introduced, and the cancer cells C floating in the inner tube 140 by the flow of the medium flowing through the opening In order to prevent escape through the opening, the medium can freely move through the opening, but there is a filtering member in the opening to limit the escape of the cancer cells (C) so that the cancer cells (C) are isolated to the inner tube (140). 142 may be formed.
한편, 상기 공급부(200)는 상기 진원부(100)의 내부를 향해 상기 배지가 공급될 수 있도록 상기 외통(120)과 연통되어 상기 배지가 상기 진원부(100)를 통과하여 이동될 수 있도록 상기 외통(120)의 상부와 연통되어 상기 배지를 공급할 수 있다.On the other hand, the supply unit 200 communicates with the outer cylinder 120 so that the medium can be supplied toward the inside of the round section 100 so that the medium can be moved through the round section 100 . It is communicated with the upper part of the outer cylinder 120 may supply the medium.
이 때, 상기 공급부(200)는 상기 내통(140)의 높이보다 상부에 위치되어 상기 외통(120)과 연통되어 있을 수 있으며, 상기 공급부(200)에서 상기 외통(120)으로 유입되는 상기 배지는 유로의 면적이 증가함에 따라 유속이 느려져 하부 방향을 향해 상기 배지의 흐름이 저속으로 유입되도록 할 수 있다.At this time, the supply unit 200 may be located above the height of the inner cylinder 140 and communicate with the outer cylinder 120, and the medium flowing into the outer cylinder 120 from the supply unit 200 is As the area of the flow path increases, the flow rate may be slowed to allow the flow of the medium to flow in the lower direction at a low speed.
한편, 상기 유도부(300)는 상기 내통(140)의 일면에 결합되어 상기 배지의 흐름에 의해 상기 내통(140)의 내부를 부유하는 상기 암세포(C)가 상기 이동부(400)를 향해 이동될 수 있도록 상기 암세포(C)가 통과할 수 있는 다수의 막으로 형성되고, 도 2에 도시된 바와 같이 제1 피막부재(320), 제2 피막부재(340), 모조혈관내피부재(360)를 포함할 수 있다.On the other hand, the induction unit 300 is coupled to one surface of the inner tube 140, the cancer cells (C) floating inside the inner tube 140 by the flow of the medium is moved toward the moving unit 400. It is formed of a plurality of membranes through which the cancer cells (C) can pass, and as shown in FIG. 2 , the first encapsulation member 320 , the second encapsulation member 340 , and the imitation blood vessel inner skin member 360 are formed. may include
여기서 상기 제1 피막부재(320)는 상기 내통(140)의 일면에 형성되어 있을 수 있다.Here, the first coating member 320 may be formed on one surface of the inner cylinder 140 .
또한, 상기 제2 피막부재(340)는 상기 제1 피막부재(320)에 접촉되도록 상기 제1 피막부재(320)와 상기 이동부(400) 사이에 구비될 수 있다.In addition, the second encapsulation member 340 may be provided between the first encapsulation member 320 and the moving part 400 so as to be in contact with the first encapsulation member 320 .
이 때, 상기 모조혈관내피부재(360)는 상기 암세포(C)가 상기 이동부(400)를 향해 이동될 수 있도록 유도물질을 분비하며, 상기 제1 피막부재(320)와 상기 제2 피막부재(340) 사이에 구비되어 상기 암세포(C)의 이동을 유도할 수 있다.At this time, the imitation blood vessel inner skin material 360 secretes an inducing material so that the cancer cells C can move toward the moving unit 400 , and the first encapsulation member 320 and the second encapsulation member It is provided between the 340 to induce the movement of the cancer cells (C).
아울러 상기 모조혈관내피부재(360)는 상기 암세포(C)가 혈관내피를 통과하면서 형질전환 되는 것을 관찰할 수 있도록 인체 내부의 혈관과 유사한 내피층을 형성할 수 있다.In addition, the imitation vascular inner skin material 360 may form an endothelial layer similar to a blood vessel inside the human body so that the cancer cells (C) can be transformed while passing through the vascular endothelium.
또한, 상기 모조혈관내피부재(360)는 상기 암세포(C)가 통과할 수 있도록 혈관 내피세포로 형성되며, 상기 제1 피막부재(320)에 부착되고, 상기 암세포(C)가 상기 이동부(400)로 이동되도록 유도물질을 분비하는 혈관내피층(362) 및 상기 혈관내피층(362)과 상게 제2 피막부재(340) 사이에서 상기 혈관내피층(362)이 상기 제2 피막부재(340)의 압력에 의해 손상되는 것을 방지하는 히드로겔층(364)을 포함할 수 있다.In addition, the imitation vascular endothelial skin material 360 is formed of vascular endothelial cells to allow the cancer cells (C) to pass through, is attached to the first encapsulation member 320, and the cancer cells (C) are moved to the moving part ( The vascular endothelial layer 362 secreting an inducer to move to 400 , and the vascular endothelial layer 362 between the vascular endothelial layer 362 and the second encapsulation member 340 is formed by the second encapsulation member 340 . ) may include a hydrogel layer 364 to prevent damage by the pressure.
여기서 상기 혈관내피층(362)은 혈관 내피세포로 구성되어 상기 암세포(C)가 인체 내부의 혈관 세포를 통과하면서 형질전환되는 것을 보다 효과적으로 관찰할 수 있도록 할 수 있으며, 혈관 세포를 통해 유도물질이 분비되어 상기 암세포(C)가 상기 이동부(400)를 향해 이동되도록 할 수 있다.Here, the vascular endothelial layer 362 is composed of vascular endothelial cells so that it can be observed more effectively that the cancer cells (C) are transformed while passing through the vascular cells inside the human body, and the inducer secreted so that the cancer cells C are moved toward the moving unit 400 .
아울러 상기 히드로겔층(364)은 상기 혈관내피층(362)이 손상되는 것을 방지하도록 상기 혈관내피층(362)과 상기 제2 피막부재(340) 사이에 구비되고, 상기 암세포(C)가 이동될 수 있도록 다수의 기공을 형성하며, 인체 내부에서 세포 외기질과 유사한 환경을 조성하여 상기 암세포(C)가 혈관 세포를 통과하여 형질전환되는 것을 보다 유사한 환경을 조성할 수 있다.In addition, the hydrogel layer 364 is provided between the vascular endothelial layer 362 and the second encapsulation member 340 to prevent the vascular endothelial layer 362 from being damaged, and the cancer cells C are moved. A plurality of pores are formed so that the cells can be transformed, and by creating an environment similar to the extracellular matrix inside the human body, it is possible to create an environment more similar to that the cancer cells (C) pass through the vascular cells and are transformed.
한편, 상기 이동부(400)는 상기 내통(140)의 일면에 결합되어 상기 유도부(300)를 통과하는 상기 암세포(C)가 이동될 수 있는 유로를 제공할 수 있고, 상기 유도부(300)와 상기 외통(120) 사이에서 상기 암세포(C)가 상기 유도부(300)를 통과하여 이동방향(B)을 따라 이동될 수 있도록 가이드하는 가이드부재(420)를 포함할 수 있다.Meanwhile, the moving unit 400 is coupled to one surface of the inner cylinder 140 to provide a flow path through which the cancer cells C passing through the induction unit 300 can move, and the induction unit 300 and A guide member 420 for guiding the cancer cells C between the outer cylinder 120 to be moved along the movement direction B through the guide part 300 may be included.
여기서 상기 가이드부재(420)는 상기 내통(140)에서 상기 외통(120)을 향해 직경이 좁아지도록 연장되어 있을 수 있다.Here, the guide member 420 may extend from the inner tube 140 toward the outer tube 120 so that the diameter becomes narrower.
상술한 구성을 바탕으로 도 3을 참조하여 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛의 활용과 기능에 대해 설명할 수 있다.Based on the above configuration, with reference to FIG. 3, the utilization and function of the measurement unit for inducing transformation of cancer cells according to an embodiment of the present invention can be described.
구체적으로 도 3은 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 배지 및 암세포의 흐름을 설명하기 위해 도시한 도면이다.Specifically, FIG. 3 is a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a medium and cancer cell flow of a cancer cell transformation observation system using the same. It is the drawing shown.
먼저 상기 공급부(200)를 통해 상기 진원부(100)를 통과하는 상기 배지는 상기 외통(120)의 상부면에 결합되어 상기 내통(140)을 향해 상기 배지가 유입되고, 상기 내통(140)의 상기 암세포(C)는 상기 배지의 유입에 의해 상기 내통(140)을 부유하며, 상기 거름부재(142)에 의해 상기 개구부로 이탈되는 것이 방지될 수 있다.First, the medium passing through the round part 100 through the supply unit 200 is coupled to the upper surface of the outer cylinder 120 so that the medium flows toward the inner cylinder 140, and of the inner cylinder 140 The cancer cells (C) float in the inner tube 140 by the inflow of the medium, and can be prevented from being separated into the opening by the filtering member 142 .
여기서 상기 배지에 의해 상기 내통(140)을 부유하는 상기 암세포(C)는 상기 유도부(300)와 인접한 위치에서 상기 혈관내피층(362)에서 분비하는 유도물질에 의해 상기 유도부(300)에서 상기 이동부(400)로 이동될 수 있다.Here, the cancer cells (C) floating in the inner tube 140 by the medium move in the induction part 300 by the inducer secreted from the vascular endothelial layer 362 at a position adjacent to the induction part 300 . It can be moved to the unit 400 .
이 때, 상기 암세포(C)는 상기 혈관내피층(362)을 통과하면서 형질전환 되고, 상기 이동부(400)를 통해 형질전환된 상기 암세포(C)가 이동되어 상기 암세포(C)를 관찰할 수 있다.At this time, the cancer cells (C) are transformed while passing through the vascular endothelial layer 362, and the transformed cancer cells (C) are moved through the moving unit 400 to observe the cancer cells (C). can
상술한 상기 측정유닛(M)의 구성을 바탕으로 도 4 내지 도 5를 참조하여 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치에 대해 설명할 수 있다.Based on the above-described configuration of the measuring unit (M), with reference to FIGS. 4 to 5, the circulating cancer cell transformation measuring apparatus according to an embodiment of the present invention can be described.
구체적으로 도 4는 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 측정장치를 설명하기 위해 도시한 도면, 도 5는 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 수집부를 설명하기 위해 도시한 도면이다.Specifically, FIG. 4 is a diagram illustrating a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a measuring device of a cancer cell transformation observation system using the same , FIG. 5 is a diagram illustrating a collection unit of a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention.
먼저 도 4에 도시된 바와 같이 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치는 전술한 암세포의 형질전환을 유도하는 측정유닛(M)을 포함하며, 상기 이동부(400)와 연통되고, 상기 이동부(400)를 따라 이동되는 상기 암세포(C) 및 상기 배지가 순환되도록 유로를 제공하는 순환유닛(500)을 포함할 수 있다.First, as shown in FIG. 4 , the circulating cancer cell transformation measuring apparatus according to an embodiment of the present invention includes the above-described measuring unit M for inducing transformation of cancer cells, and communicates with the moving unit 400 . and may include a circulation unit 500 providing a flow path to circulate the cancer cells C and the medium moving along the moving unit 400 .
여기서 상기 측정유닛(M)은 전술한 바와 동일한 구성을 가질 수 있으며, 이에 대한 자세한 설명은 생략하도록 한다.Here, the measurement unit M may have the same configuration as described above, and a detailed description thereof will be omitted.
한편, 상기 순환유닛(500)은 상기 이동부(400)와 연통되어 상기 암세포(C) 및 상기 배지가 이동되는 유로를 제공할 수 있으며, 유로상의 일부에 구비되어 상기 이동부(400)를 통해 이동되는 상기 암세포(C)를 수집하는 수집부(520) 및 유로가 적어도 하나 이상의 유로로 분할되는 서클부(540)를 포함할 수 있다.On the other hand, the circulation unit 500 communicates with the moving unit 400 to provide a flow path through which the cancer cells C and the medium are moved, and is provided in a portion of the flow path through the moving unit 400 . It may include a collecting unit 520 for collecting the moving cancer cells (C) and a circle unit 540 in which the flow path is divided into at least one flow path.
여기서 상기 수집부(520)는 유로상의 일부에 구비되어 상기 암세포(C)가 수집될 수 있도록 유로의 일부를 대체하며, 상기 암세포(C)가 추출되도록 다수의 막으로 형성되는 추출부재(522) 및 상기 추출부재(522) 사이에서 상기 암세포(C)가 수집되기 위해 유도물질을 분비하여 상기 암세포(C)가 외부로 이동을 촉진하는 촉진부재(524) 및 상기 촉진부재(524)의 외측 둘레를 따라 형성되어 상기 촉진부재(524)가 상기 추출부재(522)에 의해 손상되는 것을 방지하는 기질부재(526)를 포함할 수 있다.Here, the collection unit 520 is provided on a part of the flow path to replace a part of the flow path so that the cancer cells C can be collected, and the extraction member 522 is formed of a plurality of membranes so that the cancer cells C are extracted. and a facilitating member 524 that promotes movement of the cancer cells C to the outside by secreting an inducer to collect the cancer cells C between the extraction members 522 and the outer circumference of the facilitating member 524 It may include a substrate member 526 formed along the facilitating member 524 to prevent damage by the extraction member (522).
여기서 상기 추출부재(522)는 외피층과 내피층으로 구분되어 있을 수 있으며, 상기 암세포(C) 및 상기 배지가 이동되는 경로의 일부에 형성되어 있을 수 있다.Here, the extraction member 522 may be divided into an outer skin layer and an endothelial layer, and may be formed in a portion of a path through which the cancer cells C and the medium are moved.
아울러 상기 외피층과 상기 내피층은 상기 암세포(C)가 외부로 이동되어 통과할 수 있도록 기공이 형성되어 있을 수 있다.In addition, the outer cortical layer and the inner cortical layer may have pores so that the cancer cells C can move and pass through.
한편, 상기 촉진부재(524)는 상기 내피층의 외측 둘레를 따라 형성되어 있을 수 있으며, 유도물질을 분비하여 상기 암세포(C)가 외부로 이동되도록 촉진시킬 수 있다.Meanwhile, the promotion member 524 may be formed along the outer periphery of the endothelial layer, and secrete an inducer to promote the movement of the cancer cells C to the outside.
또한, 상기 촉진부재(524)는 앞서 상술한 상기 혈관내피층(362)과 동일한 구성, 즉, 혈관내피세포로 구성되어 있을 수 있다.In addition, the facilitating member 524 may have the same configuration as the vascular endothelial layer 362 described above, that is, may be composed of vascular endothelial cells.
한편, 상기 기질부재(526)는 상기 촉진부재(524)와 상기 외피층 사이에 형성되고, 앞서 상술한 히드로겔층(364)와 유사하게 형성될 수 있다.Meanwhile, the substrate member 526 is formed between the promotion member 524 and the outer skin layer, and may be formed similarly to the hydrogel layer 364 described above.
또한, 상기 기질부재(526)는 세포외기질과 유사한 환경을 제공할 수 있으며, 상기 암세포(C)가 외부로 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있다.In addition, the matrix member 526 may provide an environment similar to the extracellular matrix, and a plurality of pores may be formed so that the cancer cells C can move to the outside.
한편, 상기 서클부(540)는 상기 이동부(400)와 연통되며, 상기 이동부(400)와 연통되는 지점에서 적어도 하나 이상의 유로로 분할되어 있을 수 있고, 상기 암세포(C) 및 상기 배지가 순환될 수 있도록 분할된 유로가 일측에서 통합되어 폐루프를 형성할 수 있다.Meanwhile, the circle unit 540 communicates with the moving unit 400 , and may be divided into at least one flow path at a point communicating with the moving unit 400 , and the cancer cells C and the medium The divided flow paths to be circulated may be integrated from one side to form a closed loop.
보다 상세하게는 상기 서클부(540)는 곡률반경을 가지며 분할된 유로의 일단이 서로를 향해 절곡되어 하나의 유로로 통합되어 순환고리를 형성하여 폐루프를 형성할 수 있는 것이다.In more detail, the circle portion 540 has a radius of curvature, and one end of the divided flow passages is bent toward each other to be integrated into one flow passage to form a circulation ring to form a closed loop.
상술한 구성을 바탕으로 도 6 내지 도 7을 참조하여 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치에 대해 설명할 수 있다.Based on the above-described configuration, a circulating cancer cell transformation measuring apparatus according to an embodiment of the present invention can be described with reference to FIGS. 6 to 7 .
구체적으로 도 7은 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 측정유닛 내부의 상황을 설명하기 위해 도시한 도면이다.Specifically, FIG. 7 is a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an internal situation of a measuring unit of a cancer cell transformation observation system using the same. It is the drawing shown.
먼저 도 7에 도시된 바와 같이 상기 공급부(400)을 통해 유입되는 배지에 의해 상기 암세포(C)가 상기 내통(140)의 내부를 부유하면서 상기 유도부(300)와 인접한 위치로 이동되고, 상기 유도부(300)에서 분비되는 유도물질에 의해 상기 암세포(C)가 상기 이동부(400)를 향해 상기 유도부(300)를 통과하여 이동될 수 있다.First, as shown in FIG. 7 , the cancer cells C are moved to a position adjacent to the induction unit 300 while floating in the inner tube 140 by the medium introduced through the supply unit 400 , and the induction unit The cancer cells C may be moved through the induction unit 300 toward the moving unit 400 by the inducer secreted from 300 .
이 때, 상기 이동부(400)로 이동된 상기 암세포(C)는 도 6에 도시된 바와 같이 상기 서클부(540)를 통해 형성되는 폐루프를 순환할 수 있으며, 상기 순환유닛(500)의 경로 일부에 형성되는 상기 수집부(520)에 의해 외부로 이동되어 수집될 수 있다.At this time, the cancer cells C moved to the moving unit 400 may circulate a closed loop formed through the circle unit 540 as shown in FIG. 6 , and the The collection unit 520 formed in a part of the path may be moved to the outside and collected.
아울러 상기 수집부(520)에 형성되는 촉진부재(524)에서 분비되는 유도물질이 상기 서클부(540)의 순환에 의해 상기 내통(140)에 수용되는 상기 암세포(C)에 유도물질이 전달되어 상기 유도부(300)를 통과할 수 있는 확률을 높일 수 있으며, 상기 수집부(520)에서는 상기 유도부(300)에서 분비되는 유도물질에 의해 외부로 보다 효과적으로 이동되도록 할 수 있다.In addition, the inducing material secreted from the facilitating member 524 formed in the collection unit 520 is delivered to the cancer cells C accommodated in the inner tube 140 by the circulation of the circle unit 540, The probability of passing through the induction unit 300 may be increased, and the collection unit 520 may be more effectively moved to the outside by the induction material secreted from the induction unit 300 .
한편, 상기 서클부(540)의 일부에는 상기 암세포(C)가 상기 유도부(300)를 통과하여 상기 수집부(520)를 향해 이동될 수 있도록 유도물질을 추가적으로 공급하는 별도의 펌프부재가 형성될 수 있다.On the other hand, a separate pump member for additionally supplying an inducing material so that the cancer cells C can move toward the collecting unit 520 through the induction unit 300 is formed in a part of the circle unit 540 . can
상술한 구성을 바탕으로 도 8 내지 도 10을 이용하여 본 발명의 일 실시예에 따른 암세포 형질전환 관찰 시스템에 대해 설명할 수 있다.Based on the above-described configuration, the cancer cell transformation observation system according to an embodiment of the present invention can be described with reference to FIGS. 8 to 10 .
구체적으로 도 8은 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 복수의 측정유닛이 구비되는 상황을 설명하기 위해 도시한 도면, 도 9는 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 상호관계를 설명하기 위해 도시한 도면 및 도 10은 본 발명의 일 실시예에 따른 암세포의 형질전환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질전환 측정장치 및 이를 이용한 암세포 형질전환 관찰 시스템의 관찰 시스템을 설명하기 위해 도시한 도면이다.Specifically, FIG. 8 illustrates a situation in which a plurality of measuring units of a circulating cancer cell transformation measuring apparatus including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and a cancer cell transformation observation system using the same are provided. FIG. 9 is a diagram illustrating the relationship between a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells and a cancer cell transformation observation system using the same according to an embodiment of the present invention The drawings and FIG. 10 are a circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention and an observation system of a cancer cell transformation observation system using the same. It is a drawing shown for
먼저 도 8에 도시된 바와 같이 본 발명의 일 실시예에 따른 암세포의 형질전환 관찰 시스템은 상술한 바와 같은 구성을 가지는 복수의 측정유닛(M) 및 상기 복수의 측정유닛(M)과 연통되도록 유로가 분기되는 복수의 분기점이 형성되고, 상기 복수의 분기점이 서로 이어지도록 유로가 형성되어 상기 암세포 및 상기 배지가 순환되는 상기 순환유닛(500)을 포함할 수 있다.First, as shown in FIG. 8, the system for observing transformation of cancer cells according to an embodiment of the present invention includes a plurality of measurement units (M) having the configuration as described above and a flow path to communicate with the plurality of measurement units (M). A plurality of branching points are formed, and a flow path is formed so that the plurality of branching points are connected to each other, and the circulation unit 500 in which the cancer cells and the medium are circulated may include.
여기서 상기 복수의 측정유닛(M)은 상기 이동부(400)가 상기 순환유닛(500)을 향하도록 배치되어 있을 수 있으며, 도 10에 도시된 바와 같이 적어도 둘 이상이 구비되는 경우, 상기 순환유닛(500)을 기준으로 방사형으로 배치되어 있을 수 있다.Here, the plurality of measurement units M may be arranged such that the moving unit 400 faces the circulation unit 500, and when at least two or more are provided as shown in FIG. 10, the circulation unit It may be radially arranged with respect to (500).
이 때, 상기 순환유닛(500)은 앞서 상술한 구성과 동일할 수 있으며, 상기 복수의 측정유닛(M1, M2)와 연통되기 위해 유로가 분기되는 복수의 분기점이 형성되어 있을 수 있다.At this time, the circulation unit 500 may be the same as the above-described configuration, and a plurality of branch points through which a flow path is branched to communicate with the plurality of measurement units M1 and M2 may be formed.
상술한 상기 복수의 측정유닛(M1, M2)는 상기 순환유닛(500)을 통해 상기 배지 및 상기 암세포(C) 또는 유도물질을 서로 상호교환 될 수 있으며, 이를 통해 상기 제1 측정유닛(M1)과 상기 제2 측정유닛(M2)은 상기 내통(140)에 수용된 상기 암세포(C)가 상기 유도부(300)를 통과하여 상기 이동부(400)를 향해 이동되는 것을 촉진시킬 수 있다.The plurality of measurement units (M1, M2) described above may exchange the medium and the cancer cells (C) or inducers with each other through the circulation unit 500, through which the first measurement unit (M1) And the second measurement unit (M2) can promote the movement of the cancer cells (C) accommodated in the inner tube 140, through the induction unit 300 toward the moving unit (400).
또한, 상술한 바와 같이 상기 수집부(520)에 의해 상기 제1 측정유닛(M1) 및 상기 제2 측정유닛(M2) 각각에 상기 촉진부재(524)에서 분비하는 유도물질에도 영향을 받아 상기 암세포(C)의 이동량을 향상시킬 수 있다.In addition, as described above, the cancer cell is also affected by the inducing material secreted by the facilitating member 524 to each of the first and second measurement units M1 and M2 by the collection unit 520 . The movement amount of (C) can be improved.
이에 따라 상기 암세포(C)의 이동량이 향상됨에 따라 다수의 샘플을 확보할 수 있으며, 이는 인체 내부에서 상기 암세포(C)가 혈관벽 또는 발원지에서 이동되면서 발생되는 형질전환을 다량에 샘플을 통해 보다 효과적으로 관찰할 수 있는 효과가 있다.Accordingly, as the amount of movement of the cancer cells (C) is improved, a large number of samples can be secured, which is more effective through large amounts of samples for transformation that occurs while the cancer cells (C) move from the blood vessel wall or source in the human body. There is an observable effect.
아울러 발원지에서 전이되는 장기로의 이동은 도 9에 도시된 바와 같이 상기 제1 측정유닛(M1) 및 상기 제2 측정유닛(M2)과 같이 1 대 1 대응으로만 형성되는 것이 아닌 다수의 장기로의 전이가 발생될 수 있기 때문에 도 10에 도시된 바와 같이 하나의 발원지를 기준으로 다수의 전이지를 모식화하여 상기 순환유닛(500)을 기준으로 상기 이동부(400)가 상기 순환유닛(500)을 향하도록 방사형으로 배치되어 있을 수 있다.In addition, as shown in FIG. 9 , the movement from the source to the metastasized organ is not formed in a one-to-one correspondence like the first measurement unit M1 and the second measurement unit M2, but a plurality of organs. Since the transition of can occur, as shown in FIG. 10 , a plurality of transition sites are modeled based on one source, and the moving unit 400 is moved to the circulation unit 500 based on the circulation unit 500 . ) may be arranged radially to face.
이 때, 상기 수집부(520)는 각각의 상기 측정유닛(M)의 상기 이동부(400)와 인접한 위치에서 상기 유도부(300)를 통과한 상기 암세포(C)의 일부를 수집하여 형질전환된 상기 암세포(C)의 샘플을 소지할 수 있으며, 상기 순환유닛(500)에 구비되는 상기 수집부(520)를 통해 재차 형질전환된 또 다른 샘플을 채취할 수 있고, 도 9에 도시된 바와 같이 서로 상기 배지, 상기 암세포, 유도물질을 상호교환함에 따라 또 다른 상기 측정유닛(M)을 향해 이동되는 상기 암세포(C)를 수집하여 형질전환된 또 다른 상기 암세포(C)의 샘플을 채취할 수 있다.At this time, the collection unit 520 collects a part of the cancer cells (C) that have passed through the induction unit 300 at a position adjacent to the moving unit 400 of each of the measurement units (M) and is transformed. A sample of the cancer cell (C) may be possessed, and another transformed sample may be collected again through the collection unit 520 provided in the circulation unit 500, and as shown in FIG. As the medium, the cancer cells, and inducers are exchanged with each other, the cancer cells (C) that move toward the other measurement unit (M) are collected to collect a sample of the transformed cancer cells (C). there is.
상술한 바와 같이 다수의 상기 측정유닛(M)을 구비하여 다량의 샘플을 추출할 수 있으며, 상기 암세포(C)는 혈관벽 또는 또 다른 장기로 전이되기 위해 이동되면서 예측할 수 없는 형질전환을 일으키는 것에 대한 보다 효과적인 관찰과 샘플 채취가 가능하다는 장점이 있을 수 있다.As described above, a large amount of samples can be extracted by having a plurality of the measurement units (M), and the cancer cells (C) are moved to metastasize to the blood vessel wall or another organ to cause unpredictable transformation. There may be advantages in that more effective observation and sampling are possible.
즉, 본 발명의 일 실시예에 따른 암세포의 형질변환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질변환 측정장치 및 이를 이용한 암세포 형질변환 관찰 시스템은 암세포가 혈관벽을 통과하며 형질전환되는 현상을 관찰할 수 있는 효과가 있을 수 있다.That is, the circulating cancer cell transformation measuring device including a measuring unit for inducing transformation of cancer cells according to an embodiment of the present invention, and a cancer cell transformation observation system using the same, observe a phenomenon in which cancer cells are transformed as they pass through a blood vessel wall. There may be an effect that can be done.
한편, 본 발명의 또 다른 실시예에 따른 암세포의 형질변환을 유도하는 측정유닛이 포함되는 순환식 암세포 형질변환 측정장치 및 이를 이용한 암세포 형질변환 관찰 시스템은 원발지에서 이탈된 암세포(C)의 수집상태 및 변환을 간편하게 조절할 수 있도록 관리자의 단말기와 연동되는 센서가 구비될 수 있다.On the other hand, the circulating cancer cell transformation measurement device and the cancer cell transformation observation system using the same, including a measurement unit for inducing transformation of cancer cells according to another embodiment of the present invention, collect cancer cells (C) separated from the source A sensor interworking with the manager's terminal may be provided so that the state and transformation can be easily adjusted.
예를 들어, 상기 외통(120)으로 공급되는 상기 배지의 양을 조절하여 상기 내통(140)으로 유입되는 상기 배지의 양을 조절할 수 있으며, 이에 따라 상기 내통(140)에서 부유하는 상기 암세포(C)의 유동량이 변화되어 상기 유도부(300)를 통과하는 상기 암세포(C)의 양을 간접적으로 조절할 수 있다.For example, the amount of the medium flowing into the inner tube 140 may be adjusted by adjusting the amount of the medium supplied to the outer tube 120 , and accordingly, the cancer cells C floating in the inner tube 140 . ) may be changed to indirectly control the amount of cancer cells C passing through the induction unit 300 .
또는, 상기 내통(140)에서 상기 유도부(300)를 통과한 상기 암세포(C)의 양을 확인할 수 있도록 상기 암세포(C)를 센싱하는 센서가 상기 이동부(400)에 배치될 수 있는 것이다.Alternatively, a sensor for sensing the cancer cell C may be disposed on the moving unit 400 to check the amount of the cancer cell C that has passed through the induction unit 300 in the inner tube 140 .
또한, 상기 관리자의 단말기를 이용하여 상기 배지의 흐름 및 유속을 조절하고, 상기 내통(140)에 수용된 상기 암세포(C)의 감소량 및 상기 이동부(300)를 통과하는 상기 암세포(C)의 시간당 이동량을 파악할 수 있도록 이를 관리할 수 있다.In addition, by using the manager's terminal to control the flow and flow rate of the medium, the amount of decrease in the cancer cells (C) accommodated in the inner tube 140 and the amount of cancer cells (C) passing through the moving unit 300 per hour It can be managed so that the movement amount can be grasped.
이와 같이 상기 이동부(400)를 통과하는 상기 암세포(C)의 이동량을 확인할 수 있기 때문에 보다 많은 샘플을 채취하기 위해 배지의 유속 및 공급량을 상기 관리자의 단말기를 통해서 조절할 수 있는 간편함이 있을 수 있다.In this way, since it is possible to check the amount of movement of the cancer cells (C) passing through the moving unit 400, there may be convenience in which the flow rate and supply amount of the medium can be adjusted through the terminal of the manager in order to collect more samples. .
아울러 상기 관리자가 항시 상기 암세포(C)의 이동량을 확인하는 것에는 번거로움 또는 시간적 소요가 필요하기 때문에 이를 해결하기 위해 상기 센서로부터 상기 암세포(C)이 이동량 및 상기 배지의 공급량을 전달받아 상기 측정장치를 조절할 수 있는 관리서버를 더 구비할 수 있다.In addition, since it is cumbersome or time-consuming for the administrator to always check the amount of movement of the cancer cells (C), the amount of movement of the cancer cells (C) and the supply amount of the medium are transmitted from the sensor to solve this problem. A management server capable of controlling the device may be further provided.
여기서 상기 관리서버는 상기 배지의 유속을 확인하거나 상기 배지의 공급량을 확인할 수 있는 또 다른 센서를 구비할 수 있고, 상기 내통(140)의 상기 암세포(C)의 부유량과 침전량을 확인하거나 상기 암세포(C)의 수용량을 확인할 수 있는 또 다른 센서를 구비할 수도 있으며, 앞서 상술한 바와 같이 상기 이동부(400)를 통과하는 상기 암세포(C)를 확인하는 센서를 구비하여 상기 측정장치 내부에서 상기 암세포(C)의 이동량 및 변화량과 상기 배지를 조절하여 관리할 수도 있다.Here, the management server may be provided with another sensor that can check the flow rate of the medium or check the supply amount of the medium, and check the floating amount and sedimentation amount of the cancer cells (C) in the inner tube 140, or the cancer cells Another sensor that can check the capacity of (C) may be provided, and as described above, a sensor for checking the cancer cell (C) passing through the moving unit 400 is provided in the measuring device. It can also be managed by adjusting the amount of movement and change of cancer cells (C) and the medium.
한편, 먼저 도 11 내지 도 16을 참조하여 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템의 구성을 설명할 수 있다.On the other hand, first, with reference to FIGS. 11 to 16, the configuration of a transformed cancer cell collection device including a pseudovascular unit and a transformation collection observation system for cancer cells using the same according to an embodiment of the present invention can be described.
구체적으로. 도 11은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 포함하는 전반적인 모습을 도시한 도면, 도 12는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 모조혈관유닛을 설명하기 위해 도시한 도면, 도 13은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 피막부를 설명하기 위해 도시한 도면, 도 14는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 원발유닛을 설명하기 위해 도시한 도면, 도 15는 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 촉진부를 설명하기 위해 도시한 도면, 도 16은 본 발명의 일 실시예에 따른 순환식 암세포 형질전환 측정장치 및 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포 형질전환 관찰 시스템의 추출장치를 설명하기 위해 도시한 도면이다.Specifically. 11 is an overall view of a cancer cell transformation observation system using a circulating cancer cell transformation measuring device and a transformed cancer cell collection device including a dummy vascular unit, according to an embodiment of the present invention, including a dummy vascular unit. FIG. 12 is a diagram illustrating the imitation vascular unit of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the dummy vascular unit according to an embodiment of the present invention. 13 is a diagram illustrating the capsule part of the cancer cell transformation observation system using the transformed cancer cell collection device including the circulating cancer cell transformation measuring device and the imitation blood vessel unit according to an embodiment of the present invention; 14 is a view illustrating a primary unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention; FIG. 15 is a view for explaining the facilitation unit of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a dummy vascular unit according to an embodiment of the present invention, FIG. 16 is It is a diagram illustrating an extraction device of a cancer cell transformation observation system using a transformed cancer cell collection device including a circulating cancer cell transformation measuring device and a imitation blood vessel unit according to an embodiment of the present invention.
여기서 도 11에 도시된 바와 같이 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템은 모조혈관유닛(600), 원발유닛(700), 수용유닛(800), 추출장치(900), 순환장치(1000)로 구성되어 있을 수 있으며, 상기 원발유닛(700)에는 암세포(C)가 수용되어 있을 수 있다.Here, as shown in FIG. 11 , the transformed cancer cell collection device including the dummy vascular unit according to an embodiment of the present invention and the transformation collection and observation system for cancer cells using the same are the imitation vascular unit 600 and the primary unit 700 . ), the receiving unit 800 , the extraction device 900 , and the circulation device 1000 may be configured, and the cancer cell C may be accommodated in the primary unit 700 .
이 때, 상기 모조혈관유닛(600)은 도 12에 도시된 바와 같이 상기 암세포(C)가 상기 원발유닛(700) 내부에서 이동되는 주입구(I)와 상기 모조혈관유닛(600)을 통과하여 이동되는 배출구(O)의 경로상의 일부에 형성될 수 있다.At this time, as shown in FIG. 12 , the imitation vascular unit 600 moves through the injection hole I through which the cancer cells C move inside the primary unit 700 and the imitation vascular unit 600 . It may be formed in a part of the path of the outlet (O).
아울러 상기 모조혈관유닛(600)은 상기 암세포(C)의 이동경로상에서 복수개가 서로 이격되도록 경로를 따라 배치되어 상기 암세포(C)가 외부로 유출되도록 이격된 틈(622)을 형성하는 고리부(620)와 내부에 복수의 상기 고리부(620)가 위치되고, 다수의 막으로 형성되며, 상기 이격된 틈(622)에서 상기 암세포(C)가 외부로 유출되도록 유도물질을 분비하고, 상기 배지 및 상기 암세포(C)가 이동하는 경로가 되는 피막부(640) 및 상기 고리부(620)를 서로 연결하는 연결부(660)를 포함할 수 있다.In addition, the imitation blood vessel unit 600 is arranged along the path so that a plurality of them are spaced apart from each other on the movement path of the cancer cells (C) to form a gap 622 spaced apart so that the cancer cells (C) are discharged to the outside ( 620) and a plurality of the ring parts 620 are positioned therein, are formed of a plurality of membranes, and secrete an inducer so that the cancer cells (C) are discharged to the outside in the spaced gap 622, and the medium And it may include a connecting portion 660 for connecting the coating portion 640 and the ring portion 620 that is a path through which the cancer cells (C) move.
여기서 상기 고리부(620)는 상기 주입구(I)에서 상기 배출구(O)를 향해 복수개가 배열되어 서로 이격되어 있을 수 있으며, 복수의 상기 고리부(620)는 서로 기 설정된 거리만큼 이격되어 있을 수 있다.Here, a plurality of the ring portions 620 may be spaced apart from each other by being arranged from the inlet (I) toward the outlet (O), and the plurality of ring portions 620 may be spaced apart from each other by a predetermined distance. there is.
또한, 상기 고리부(620)는 서로 이격되어 상기 이격된 틈(622)을 형성하여 상기 주입구(I)에서 상기 배출구(O)로 이동되는 상기 암세포(C)가 외부로 유출되도록 할 수 있다.In addition, the ring portion 620 may be spaced apart from each other to form the spaced gap 622 so that the cancer cells C moving from the inlet I to the outlet O may flow out.
아울러 상기 고리부(620)는 다수의 막으로 형성되는 상기 피막부(640)가 중력에 의해 하부를 향해 늘어나는 것을 방지하기 위해 상기 피막부(640)의 내측과 접촉되어 상기 피막부(640)를 지지할 수 있다.In addition, the ring part 620 is in contact with the inner side of the coating part 640 to prevent the coating part 640 formed of a plurality of films from stretching downward by gravity to close the coating part 640 . can support
한편, 상술한 상기 피막부(640)는 다수의 막으로 형성되며, 상기 암세포(C)가 외부로 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있으며 도 13에 도시된 바와 같이, 덮개부재(642), 분비부재(644), 수분유지부재(646)로 구성될 수 있다.On the other hand, the above-described coating portion 640 is formed of a plurality of membranes, a plurality of pores may be formed so that the cancer cells (C) can move to the outside, and as shown in FIG. 13 , the cover member ( 642 ), a secretion member 644 , and a moisture retention member 646 .
여기서 상기 덮개부재(642)는 상기 고리부(620) 및 상기 연결부(660)를 감싸도록 구비되며, 상기 고리부(620)의 외측 둘레를 따라 형성되어 있을 수 있다.Here, the cover member 642 is provided to surround the ring portion 620 and the connection portion 660 , and may be formed along the outer circumference of the ring portion 620 .
또한, 앞서 상술한 바와 같이 상기 암세포(C)가 외부로 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있다.In addition, as described above, a plurality of pores may be formed so that the cancer cells C can move to the outside.
한편, 상기 분비부재(644)는 상기 암세포(C)가 상기 이격된 틈(622)에서 상기 피막부(640)를 통과하여 외부로 유출될 수 있도록 상기 암세포(C)의 유출을 유도하는 유도물질을 분비할 수 있다.On the other hand, the secretion member 644 is an inducing material for inducing the outflow of the cancer cells (C) so that the cancer cells (C) can flow out of the gap (622) through the membrane portion (640) to the outside. can secrete
또한, 상기 분비부재(644)는 상기 덮개부재(642)의 외측 둘레를 따라 형성되어 있을 수 있고, 상기 암세포(C)가 혈관벽을 통과하며 형질변환되는 것을 관찰할 수 있도록 혈관내피세포층으로 형성되어 있을 수 있다.In addition, the secretion member 644 may be formed along the outer periphery of the cover member 642, and is formed as a vascular endothelial cell layer so that the cancer cells (C) can be observed to be transformed while passing through the blood vessel wall. there may be
여기서 상술한 상기 덮개부재(642) 및 상기 분비부재(644)는 상기 주입구(I)에서 상기 배출구(O)를 향해 길게 형성되어 있을 수 있다.Here, the above-described cover member 642 and the secretion member 644 may be formed to be elongated from the inlet (I) toward the outlet (O).
한편, 상기 수분유지부재(646)는 상기 분비부재(644)의 수분이 증발하여 상기 암세포(C)의 이동이 제한되는 것을 방지하기 위해 상기 분비부재(644)의 수분을 유지할 수 있도록 상기 분비부재(644)의 외측 둘레를 따라 형성되어 있을 수 있다.On the other hand, the moisture retention member 646 is the secretion member to maintain moisture in the secretion member 644 in order to prevent the moisture of the secretion member 644 from being restricted from the movement of the cancer cells (C) is limited. It may be formed along the outer perimeter of 644 .
이 때, 상기 수분유지부재(646)는 상술한 상기 이격된 틈(622) 사이에만 구비되어 상기 분비부재(644)의 둘레를 따라 형성될 수 있으며, 이는 상기 암세포(C)가 유출되는 상기 이격된 틈(622)의 상기 분비부재(644)의 수분을 유지하여 상기 암세포(C)의 이동이 제한되지 않도록 할 수 있는 것이다.At this time, the moisture retaining member 646 is provided only between the above-described spaced gaps 622 and may be formed along the circumference of the secretion member 644, which is the spacing at which the cancer cells C are discharged. It is possible to keep the moisture in the secretion member 644 in the gap 622 so that the movement of the cancer cells C is not restricted.
아울러 상기 수분유지부재(646)는 상기 암세포(C)가 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있으며, 기질층으로 형성되어 혈관 사이에 존재하는 세포외기질과 유사한 환경을 제공할 수 있다.In addition, the moisture retention member 646 may have a plurality of pores formed so that the cancer cells C can move, and is formed as a matrix layer to provide an environment similar to the extracellular matrix existing between blood vessels. .
즉, 상기 수분유지부재(646)는 상기 분비부재(644)의 수분을 유지하여 상기 이격된 틈(622)에서 상기 암세포(C)가 이동되는 것이 제한되지 않도록 하며, 상기 이격된 틈(622)을 통해 유출되는 상기 암세포(C)를 인체와 유사한 환경을 제공할 수 있도록 세포외기질과 유사한 환경을 조성하고, 다수의 기공을 형성하여 상기 암세포(C)가 상기 이격된 틈(622)을 통해 유출될 수 있도록 할 수 있다.That is, the moisture retention member 646 maintains the moisture of the secretion member 644 so that the movement of the cancer cells C is not limited in the spaced gap 622 , and the spaced gap 622 . An environment similar to the extracellular matrix is created to provide an environment similar to the human body for the cancer cells (C) flowing out through the may be allowed to leak.
한편, 상기 연결부(660)는 상기 고리부(620)의 둘레 일부가 인접한 상기 고리부(620)를 향해 연장되어 서로를 연결하고 있을 수 있다.On the other hand, the connecting portion 660 may be connected to each other by extending a portion of the circumference of the ring portion 620 toward the adjacent ring portion (620).
아울러 상기 연결부(660)는 인접한 상기 고리부(620)를 연결하여 복수의 상기 고리부(620) 사이의 간격을 유지하고, 상기 주입구(I)에서 상기 배출구(O)를 향해 길게 형성되어 복수의 상기 고리부(620)가 경로상에 위치될 수 있도록 고정시킬 수 있다.In addition, the connecting portion 660 connects the adjacent ring portions 620 to maintain a distance between the plurality of ring portions 620, and is formed elongated from the inlet (I) toward the outlet (O) to form a plurality of The ring portion 620 may be fixed to be positioned on the path.
이 때, 상기 연결부(660)는 상기 고리부(620)의 둘레를 따라 다수개가 형성되어 있을 수 있으며, 상기 암세포(C)가 유출되는 상기 이격된 틈(622)의 면적을 향상시키기 위해 서로 마주보도록 한 쌍이 마련되는 것이 바람직할 수 있다.At this time, a plurality of the connection parts 660 may be formed along the circumference of the ring part 620 and face each other to improve the area of the spaced gap 622 through which the cancer cells C are discharged. It may be desirable to provide a pair for viewing.
한편, 상기 암세포(C)가 상기 모조혈관유닛(600)으로 이동될 수 있도록 도 14에 도시된 바와 같이 상기 암세포(C)가 수용되는 상기 원발유닛(700)은 상기 주입구(I) 및 상기 배출구(O)와 연통되어 상기 배지가 순환되는 순환부(720), 상기 순환부(720)와 연통되는 외부 하우징(742)과 상기 외부 하우징(742)과 이격되도록 형성되는 내부 하우징(744)으로 구분되는 발상부(740) 및 상기 내부 하우징(744)의 일면에 형성되어 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동될 수 있도록 유도물질을 분비하는 촉진부(760)로 구성될 수 있다.On the other hand, as shown in FIG. 14 , the primary unit 700 in which the cancer cells C are accommodated has the inlet I and the outlet so that the cancer cells C can move to the imitation vascular unit 600 . (O) is divided into a circulation unit 720 through which the medium is circulated, an outer housing 742 communicating with the circulation unit 720, and an inner housing 744 formed to be spaced apart from the outer housing 742 It is formed on one surface of the propulsion part 740 and the inner housing 744 to be composed of a facilitating part 760 that secretes an inducer so that the cancer cells C can move toward the imitation blood vessel unit 600 . can
여기서 상기 순환부(720)는 상기 주입구(I) 및 상기 배출구(O)와 연통되어 있을 수 있고, 경로상의 일부에 상기 배지를 공급할 수 있는 배지 펌프 또는 상기 배지의 이동동력을 제공하는 펌프가 구비될 수 있다.Here, the circulation unit 720 may be in communication with the inlet (I) and the outlet (O), and a medium pump capable of supplying the medium to a part of the path or a pump providing the movement power of the medium is provided. can be
한편, 상기 발상부(740)는 상기 외부 하우징(742)과 상기 내부 하우징(744)으로 구분될 수 있으며, 상기 외부 하우징(742)과 상기 내부 하우징(744)은 서로 이격되도록 배치될 수 있다.On the other hand, the propulsion part 740 may be divided into the outer housing 742 and the inner housing 744, and the outer housing 742 and the inner housing 744 may be disposed to be spaced apart from each other.
여기서 상기 외부 하우징(742)은 상기 배지가 유입될 수 있도록 상기 순환부(720)와 연통되어 있을 수 있으며, 상기 순환부(720)가 상기 외부 하우징(742)의 상부에 연통되어 상기 배지가 유입되면서 자연스럽게 상기 내부 하우징(744)으로 유입되도록 할 수 있다.Here, the outer housing 742 may be in communication with the circulation unit 720 so that the medium can be introduced, and the circulation unit 720 is communicated with the upper part of the outer housing 742 to allow the medium to flow in. while being naturally introduced into the inner housing 744 .
이 때, 상기 내부 하우징(744)은 상기 외부 하우징(742)보다 낮은 높이를 갖도록 형성되며, 상기 배지가 유입될 수 있도록 상부를 향해 개구부가 형성되고, 내부에는 상기 암세포(C)가 수용되어 내부로 유입되는 상기 배지에 의해 상기 암세포(C)가 상기 내부 하우징(744) 내부에서 부유될 수 있다.At this time, the inner housing 744 is formed to have a lower height than the outer housing 742 , an opening is formed upward to allow the medium to flow in, and the cancer cells C are accommodated therein. The cancer cells (C) may be suspended in the inner housing 744 by the medium introduced into the inner housing 744 .
아울러 상기 내부 하우징(744)의 개구부에는 상기 배지에 의해 상기 내부 하우징(744)의 내부를 부유하는 상기 암세포(C)가 상기 개구부를 통해 상기 외부 하우징(742)으로 유출되는 것을 방지하기 위해 상기 개구부에 상기 암세포(C)의 이탈을 방지하는 거름부재(746)가 구비될 수 있다.In addition, the opening of the inner housing 744 has the opening in order to prevent the cancer cells C floating inside the inner housing 744 by the medium from flowing out to the outer housing 742 through the opening. A filtering member 746 for preventing the cancer cells C from leaving may be provided.
여기서 상기 거름부재(746)는 상기 배지가 상기 내부 하우징(744)을 향해 이동하는 것은 제한하지 않고, 상기 암세포(C)가 상기 내부 하우징(744)에서 이탈하는 것만을 제한하여, 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동될 수 있는 환경을 조성할 수 있다.Here, the filtering member 746 does not limit the movement of the medium toward the inner housing 744, but restricts the cancer cells C from leaving the inner housing 744, so that the cancer cells C ) can create an environment in which the imitation blood vessel unit 600 can be moved.
한편, 상기 촉진부(760)는 도 15에 도시된 바와 같이 다수의 막으로 형성되어 있을 수 있으며, 상기 내부 하우징(744)의 일면에 결합하는 제1 상피부재(762), 상기 제1 상피부재(762)와 접하도록 구비되는 제2 상피부재(764) 및 상기 제1 상피부재(762)와 상기 제2 상피부재(764) 사이에 구비되는 제3 상피부재(766)으로 구성될 수 있다.On the other hand, the promotion part 760 may be formed of a plurality of films as shown in FIG. 15 , and a first upper skin member 762 and the first upper skin member coupled to one surface of the inner housing 744 . It may include a second upper skin member 764 provided to be in contact with 762 , and a third upper skin member 766 provided between the first upper skin member 762 and the second upper skin member 764 .
여기서 상기 제1 상피부재(762)는 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있으며, 상기 내부 하우징(744)의 일면을 형성하고 있을 수 있다.Here, the first upper skin material 762 may have a plurality of pores formed so that the cancer cells C can move toward the imitation vascular unit 600, and form one surface of the inner housing 744, there may be
아울러 상기 제2 상피부재(764)는 상술한 제1 상피부재(762)와 유사하게 다수의 기공이 형성되어 있을 수 있으며, 둘레가 상기 제1 상피부재(762)와 접하도록 구비될 수 있다.In addition, the second upper skin member 764 may have a plurality of pores formed similarly to the above-described first upper skin member 762 , and the periphery may be provided so as to be in contact with the first upper skin member 762 .
이 때, 상기 제3 상피부재(766)는 상기 제1 상피부재(762)와 상기 제2 상피부재(764) 사이에서 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동될 수 있도록 유도물질을 분비하는 모조피막층(F1), 상기 제2 상피부재(764)에 의해 상기 모조피막층(F1)이 손상되는 것을 방지하는 기질층(F2)으로 구성되어 있을 수 있다.At this time, the third epidermis 766 is disposed between the first epidermis 762 and the second epidermis 764 so that the cancer cells C can move toward the imitation vascular unit 600 . It may be composed of a imitation coating layer (F1) that secretes an inducer, and a matrix layer (F2) that prevents the imitation coating layer (F1) from being damaged by the second epidermal material (764).
여기서 상기 모조피막층(F1)은 혈관내피세포로 구성되어 있을 수 있으며, 상기 암세포(C)가 최초 발생한 원발지에서 이탈하는 것을 모식화할 수 있도록 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동하도록 유도물질을 분비하며, 상기 내부 하우징(644)을 부유하는 상기 암세포(C)가 인접한 위치로 이동되면 유도물질에 의해 상기 모조혈관유닛(600)을 향해 이동되도록 유도할 수 있다.Here, the imitation capsular layer (F1) may be composed of vascular endothelial cells, and the cancer cells (C) form the imitation vascular unit (600) so as to model the departure from the original site in which the cancer cells (C) are first generated. Inducing material is secreted to move toward, and when the cancer cells C floating in the inner housing 644 are moved to an adjacent position, it can be induced to move toward the pseudovascular unit 600 by the inducing material.
아울러 상기 기질층(F2)은 상기 제1 상피부재(762) 및 상기 제2 상피부재(764)의 압력에 의해 상기 모조피막층(F1)이 손상되는 것을 방지하도록 상기 모조피막층(F1)과 상기 제2 상피부재(764) 사이에 구비될 수 있으며, 상기 암세포(C)가 상기 모조혈관유닛(600)을 향해 이동될 수 있도록 다수의 기공이 형성되어 있을 수 있으며, 히드로겔로 형성되어 인체 내부에서 혈관 사이에 구비되는 세포외기질과 유사한 환경을 조성할 수도 있다.In addition, the substrate layer (F2) comprises the imitation coating layer (F1) and the imitation coating layer (F1) to prevent the imitation coating layer (F1) from being damaged by the pressure of the first and second upper skin members (762) and the second upper layer (764). It may be provided between the two upper skin materials 764 , and a plurality of pores may be formed so that the cancer cells C can move toward the imitation vascular unit 600 , and are formed of a hydrogel to form a hydrogel inside the human body. It is also possible to create an environment similar to the extracellular matrix provided between blood vessels.
즉, 상기 기질층(F2)은 상기 모조피막층(F1)의 손상을 방지하면서, 상기 암세포(C)가 이동될 수 있는 다수의 기공을 형성하고, 상기 암세포(C)의 이동을 보다 인체와 유사하게 조성하기 위해 세포외기질과 유사한 환경을 조성할 수 있는 것이다.That is, the stromal layer (F2) prevents damage to the imitation coating layer (F1), forms a plurality of pores through which the cancer cells (C) can move, and makes the movement of the cancer cells (C) more similar to that of the human body. It is possible to create an environment similar to the extracellular matrix in order to create
한편, 상기 수용유닛(800)은 상기 암세포(C)가 상기 모조혈관유닛(600)에 형성되는 상기 이격된 틈(622)을 통해 외부로 유출되는 경우, 상기 암세포(C)를 수용할 수 있도록 상기 모조혈관유닛(600)이 내부에 위치하며, 상기 원발유닛(700)이 상기 주입구(I) 및 상기 배출구(O)와 연통될 수 있도록 상기 순환부(720)가 관통하도록 형성되어 있을 수 있다.On the other hand, the receiving unit 800 is configured to accommodate the cancer cells (C) when the cancer cells (C) flow out through the spaced gap (622) formed in the imitation blood vessel unit (600). The imitation blood vessel unit 600 is located therein, and the circulation unit 720 may be formed to pass through so that the primary unit 700 can communicate with the inlet (I) and the outlet (O). .
또한, 상기 모조혈관유닛(600)을 통해 상기 수용유닛(800) 내부로 유출되는 상기 암세포(C)의 이동을 촉진하도록 내부에는 유도물질이 수용되어 있을 수 있다.In addition, an inducer may be accommodated therein to promote the movement of the cancer cells (C) flowing out into the receiving unit 800 through the imitation blood vessel unit 600 .
한편, 상기 추출장치(900)는 도 16에 도시된 바와 같이 상기 모조혈관유닛(600)에서 유출된 상기 암세포(C)가 상기 수용유닛(800)에 수용되는 유도물질에 의해 이동되면서 추출될 수 있도록 내부 공간이 형성될 수 있으며, 유도물질이 통과하는 이동영역(920)과 상기 암세포(C)가 침전되는 침전영역(940)으로 구분될 수 있다.On the other hand, the extraction device 900 can be extracted while moving by the inducing material accommodated in the receiving unit 800, the cancer cells (C) leaked from the imitation blood vessel unit 600, as shown in FIG. An inner space may be formed so that the inducing material passes therethrough and a sedimentation area 940 in which the cancer cells C are precipitated may be divided.
여기서 상기 이동영역(920)은 상기 순환장치(1000)를 통해 상기 수용유닛(800)과 연통되어 있을 수 있으며, 상기 추출장치(900)의 상부영역에 형성되어 유도물질과 함께 이동되는 상기 암세포(C)가 유도물질의 흐름에 의해 유속이 느려지는 영역일 수 있다.Here, the moving region 920 may be in communication with the receiving unit 800 through the circulation device 1000, and the cancer cells ( C) may be a region where the flow rate is slowed by the flow of the inducer.
이는 유속의 면적이 상기 순환장치(1000)보다 상기 이동영역(920)이 넓어지기 때문에 유속이 느려질 수 있으며, 유도물질의 유속이 느려지면서 상기 암세포(C)는 상기 침전영역(940)으로 침전될 수 있다.This is because the area of the flow rate is wider than that of the circulation device 1000 in the moving region 920 , so the flow rate may be slowed. can
한편, 상기 침전영역(940)은 상기 순환장치(1000)를 통해 이동된 상기 암세포(C)가 상기 이동영역(920)에서 유속이 느려지는 유도물질에 의해 상대적으로 유도물질보다 무거운 상기 암세포(C)가 상기 침전영역(940)을 향해 침전되어 상기 암세포(C)가 추출될 수 있다.On the other hand, in the sedimentation region 940 , the cancer cells C moved through the circulation device 1000 are relatively heavier than the cancer cells C due to the inducing material whose flow rate is slowed in the movement region 920 . ) is precipitated toward the precipitation region 940 so that the cancer cells C can be extracted.
또한, 상기 침전영역(940)은 상기 이동영역(920)과 연통되는 부분이 단턱지게 형성되어 있을 수 있으며, 이를 통해 상기 이동영역(920)과 상기 침전영역(940)이 구분될 수 있다.In addition, the sedimentation region 940 may have a stepped portion communicating with the moving region 920 , through which the moving region 920 and the sedimentation region 940 may be divided.
한편, 앞서 상술한 바와 같이 상기 순환장치(1000)는 상기 추출장치(900)와 연통되어 있을 수 있으며, 상기 이동영역(920)과 연통되기 위해 상기 추출장치(900)의 상부에 연통되어 있을 수 있다.On the other hand, as described above, the circulation device 1000 may be in communication with the extraction device 900 , and may be in communication with the upper portion of the extraction device 900 to communicate with the moving region 920 . there is.
또한, 상기 순환장치(1000)는 유도물질의 동력을 제공할 수 있도록 경로상의 일부에 펌프부가 구비되어 있을 수 있다.In addition, the circulation device 1000 may be provided with a pump unit in a portion of the path to provide the power of the inducing material.
앞서 상술한 구성을 바탕으로 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포 형질전환 수집 관찰 시스템의 전반적인 상황에 대해 도 17 내지 도 20을 참조하여 설명할 수 있다.Based on the above-described configuration, the overall situation of the transformed cancer cell collection device including the pseudovascular unit and the cancer cell transformation collection and observation system using the same according to an embodiment of the present invention will be described with reference to FIGS. 17 to 20 . can do.
먼저 도 17에 도시된 바와 같이 상기 내부 하우징(744)에 수용되는 상기 암세포(C)는 앞서 상술한 바와 같이 상기 내부 하우징(744)으로 유입되는 배지에 의해 상기 내부 하우징(744) 내부를 부유하게 되고, 상기 암세포(C)가 상기 촉진부(760)와 인접한 위치에서 상기 모조피막층(F1)의 유도물질에 의해 상기 모조혈관유닛(600)을 향해 이동될 수 있다.First, as shown in FIG. 17, the cancer cells C accommodated in the inner housing 744 float inside the inner housing 744 by the medium introduced into the inner housing 744 as described above. and the cancer cells C may be moved toward the imitation blood vessel unit 600 by the inducing material of the imitation film layer F1 at a position adjacent to the promotion part 760 .
여기서 상기 암세포(C)는 상술한 바와 같이 상기 모조혈관유닛(600)의 상기 이격된 틈(622)을 통해서 상기 수용유닛(800) 내부에 이동될 수 있으며, 상기 주입구(I) 및 상기 배출구(O)는 상기 원발유닛(700)과 연통되도록 상기 수용유닛(800)을 관통하고 있을 수 있다.Here, the cancer cell (C) can be moved inside the receiving unit 800 through the spaced gap 622 of the imitation vascular unit 600 as described above, and the inlet (I) and the outlet ( O) may pass through the receiving unit 800 so as to communicate with the primary unit 700 .
이 때, 상기 암세포(C)의 일부가 상기 이격된 틈(622)을 통해 유출되면, 상기 순환장치(1000)에 의해 유도물질과 함께 상기 추출장치(900)로 이동될 수 있다.At this time, when a part of the cancer cells C flows out through the spaced gap 622 , it may be moved to the extraction device 900 together with the inducer by the circulation device 1000 .
여기서 상기 수용유닛(800)의 하부 바닥면에는 상기 암세포(C)가 상기 수용유닛(800) 내부로 이동되는 것을 보다 촉진시키기 위해 상기 모조피막층(F1)과 동일한 구성의 모조피막층(F1)이 바닥면에 구비될 수 있다.Here, on the lower bottom surface of the accommodating unit 800, a imitation film layer (F1) having the same configuration as the imitation film layer (F1) is at the bottom in order to more facilitate the movement of the cancer cells (C) into the accommodation unit (800). It may be provided on the side.
한편, 유도물질은 상기 추출유닛(900) 내부에서 상기 이동영역(920)에 의해 유도물질의 유속이 느려지고, 이에 따라 상대적으로 무거운 상기 암세포(C)가 상기 침전영역(940)으로 침전되어 상기 암세포(C)가 추출될 수 있는 것이다.On the other hand, the flow rate of the inducing material is slowed by the moving region 920 inside the extraction unit 900, and accordingly, the relatively heavy cancer cells C are precipitated into the precipitation region 940 and the cancer cells. (C) can be extracted.
이 때, 상기 모조혈관유닛(600)에서 유출되는 상기 암세포(C)는 도 19에 도시된 바와 같이 상기 이격된 틈(622)을 통해 유출될 수 잇으며, 상기 고리부(620) 사이에서 상기 피막부(620)를 통과할 수 있는 것이다.At this time, the cancer cells (C) flowing out from the imitation blood vessel unit 600 may flow out through the spaced gap 622 as shown in FIG. 19, and between the ring parts 620, the It can pass through the coating portion (620).
이는 앞서 상술한 바와 같이 상기 분비부재(644)가 분비하는 유도물질 또는 상기 수용유닛(800) 내부에 수용되는 유도물질에 의해 이동이 촉진될 수 있으며, 상기 덮개부재(642), 상기 분비부재(644), 상기 수분유지부재(646)에 형성되는 기공을 통과하여 상기 수용유닛(800) 내부로 이동될 수 있는 것이다.As described above, the movement can be facilitated by the inducing material secreted by the secretory member 644 or the inducing material accommodated in the receiving unit 800, and the cover member 642, the secretory member ( 644), passing through the pores formed in the moisture retaining member 646, it will be able to move into the receiving unit (800).
또한, 상기 수분유지부재(646)는 상기 암세포(C)의 이동이 제한되지 않도록 상기 분비부재(644)의 수분을 유지하기 위해 상기 이격된 틈(622)의 상기 분비부재(644)의 둘레를 따라 형성되어 있을 수 있다.In addition, the moisture retaining member 646 surrounds the secretion member 644 of the spaced gap 622 to retain moisture in the secretion member 644 so that the movement of the cancer cells C is not restricted. may be formed accordingly.
한편, 상기 모조혈관유닛(600)이 길게 형성되어 중력에 의해 하부를 향해 늘어지는 것을 방지하기 위해 상기 이격된 틈(622)에는 서로 마주보는 상기 연결부(660)를 이어 상기 모조혈관유닛(600)의 강성을 보강하는 보강부(680)가 형성될 수 있다.On the other hand, in order to prevent the imitation blood vessel unit 600 from being elongated and drooping downward by gravity, the imitation blood vessel unit 600 is connected to the connecting parts 660 facing each other in the spaced gap 622 . A reinforcing part 680 for reinforcing the rigidity of may be formed.
여기서 상기 보강부(680)는 서로 마주보는 상기 연결부(660)를 연결하도록 상기 이격된 틈(622)를 가로지르도록 형성되어 있을 수 있으며, 상기 이격된 틈(622)이 폐쇄되지 않도록 상기 고리부(620)의 둘레 굴곡을 따라 곡률반경을 가지도록 형성될 수 있다.Here, the reinforcing part 680 may be formed to cross the spaced gap 622 to connect the connecting parts 660 facing each other, and the ring part so that the spaced gap 622 is not closed. It may be formed to have a radius of curvature along the circumferential curve of 620 .
아울러 도 20에 도시된 바와 같이 서로 어긋나도록 상기 주입구(I)에서 상기 배출구(O)를 향해 순차적으로 배치되어 있을 수 있으며, 동일한 방향을 향해 배치되어 있을 수도 있고, 서로 교차되도록 배치되어 있을 수도 있다.In addition, as shown in FIG. 20, they may be sequentially arranged from the inlet (I) toward the outlet (O) so as to be shifted from each other, may be arranged in the same direction, or may be arranged to cross each other .
다만, 상기 이격된 틈(622)이 폐쇄되어 상기 암세포(C)가 유출되는 것에 제한되지 않도록 상기 이격된 틈(622)을 개방하도록 형성되어 있을 수 있다.However, the spaced gap 622 may be closed to open the spaced gap 622 so that the cancer cells C are not limited to outflow.
상술한 구성을 바탕으로 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템은 상기 암세포(C)가 최초 발생지에서 이탈하여 혈관을 따라 이동하며, 혈관벽을 통과해 전이부를 통해 이동되는 것을 모식화할 수 있고, 이를 통해 혈관벽 또는 최초 발생지에서 이탈하면서 형질전환되는 것을 관찰하도록 다수의 샘플을 채취할 수 있으며, 이에 따라 약물을 주입하는 경우 특정한 상기 암세포(C)를 목표로 하여 약물을 주입하고 이를 통해 경과를 관찰할 수 있다.Based on the above-described configuration, the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same, including the imitation vascular unit according to an embodiment of the present invention, are provided in which the cancer cells (C) deviate from the original site of origin and collect blood vessels. Moving along, passing through the blood vessel wall and moving through the metastasis can be modeled, and through this, a large number of samples can be taken to observe transformation while departing from the blood vessel wall or the original site of origin, and accordingly, when the drug is injected A drug can be injected by targeting the specific cancer cell (C), and the progress can be observed through this.
이에 따라 본 발명의 일 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템은 혈관내피세포를 통과하는 암세포(C)의 형질전환을 다양한 샘플을 채취하여 관찰할 수 있는 효과가 있을 수 있다.Accordingly, the transformed cancer cell collection device including the imitation vascular unit and the transformation collection and observation system of cancer cells using the same according to an embodiment of the present invention can perform transformation of cancer cells (C) passing through vascular endothelial cells using various samples. There may be effects that can be collected and observed.
한편, 본 발명의 또 다른 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템은 혈관내피세포를 통과하는 암세포(C)의 수집상태 및 변환을 간편하게 조절할 수 있도록 관리자의 단말기와 연동되는 센서가 구비될 수 있다.On the other hand, the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same including the imitation vascular unit according to another embodiment of the present invention monitor the collection state and transformation of cancer cells (C) passing through vascular endothelial cells. A sensor interworking with the manager's terminal may be provided for easy adjustment.
예를 들어, 상기 발상부(740)에 위치된 상기 암세포(C)가 배지의 흐름에 의해 상기 모조혈관유닛(600)을 향해 이동되는 주기 및 양을 확인하기 위해 암세포(C)를 센싱할 수 있는 제1 센서를 상기 모조혈관유닛(600)과 상기 촉진부(760) 사이에 구비하여 시간당 상기 암세포(C)의 이동량, 이동주기, 시간에 따른 상기 암세포(C)의 이동량 등을 상기 관리자의 단말기를 통해서 확인할 수 있다.For example, the cancer cells (C) can be sensed in order to check the period and amount in which the cancer cells (C) located in the kicking part 740 are moved toward the pseudovascular unit 600 by the flow of the medium. By providing a first sensor between the imitation blood vessel unit 600 and the facilitator 760, the amount of movement of the cancer cells (C) per time, the movement period, the amount of movement of the cancer cells (C) according to time, etc. of the manager You can check it through the terminal.
또는, 상기 모조혈관유닛(600)에서 상기 수용유닛(800)으로 이동된 상기 암세포(C)가 유체의 흐름에 따라 상기 추출장치(900)로 이동되는 것을 확인할 수 있도록 상기 암세포(C)를 센싱하는 제2 센서가 상기 순환장치(1000)의 경로상에 배치될 수 있는 것이다.Alternatively, the cancer cells (C) are sensed so that it can be confirmed that the cancer cells (C) moved from the imitation blood vessel unit (600) to the receiving unit (800) are moved to the extraction device (900) according to the flow of the fluid. A second sensor that can be disposed on the path of the circulation device (1000).
또한, 상기 관리자의 단말기를 이용하여 본 발명의 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템을 제어할 수 있도록 배지를 순환시키도록 동력을 제공하는 동력부와 연동되어 상기 관리자의 단말기를 통해 배지의 흐름의 강도 및 유속을 제어할 수도 있으며, 상기 펌프부와 연동되어 유도물질의 유속 및 흐름을 조절하여 상기 암세포(C)가 상기 추출장치(1000) 내부에서 상기 침전영역(940)으로 효과적으로 침전되도록 조절할 수 있는 것이다.In addition, a power unit that provides power to circulate the medium so as to control the transformed cancer cell collection device including the imitation blood vessel unit of the present invention and the transformation collection and observation system of cancer cells using the same by using the manager's terminal It is also possible to control the intensity and flow rate of the flow of the medium through the manager's terminal in conjunction with the pump unit to control the flow rate and flow of the inducing material so that the cancer cells (C) are inside the extraction device 1000 It can be controlled so that it is effectively precipitated into the precipitation region 940 in the .
아울러 상기 제1 센서 및 상기 제2 센서를 통해 측정된 데이터를 관리하는 관리서버가 구비되어 상기 관리서버거 상기 제1 센서 및 상기 제2 센서를 통해 측정된 데이터를 수신하여 시간, 상기 동력부 및 상기 펌프부의 세기, 싱가 원발유닛(700) 내부에 수용된 암세포(C) 양에 따른 상기 암세포(C)의 이동량을 분석하도록 할 수 있으며, 상기 동력부 및 상기 펌프부와 연동되어 배지 또는 유도물질을 공급하는 공급부를 제어할 수 있도록 구비될 수도 있다.In addition, a management server for managing data measured through the first sensor and the second sensor is provided, and the management server receives the data measured through the first sensor and the second sensor to determine the time, the power unit and It is possible to analyze the amount of movement of the cancer cells (C) according to the strength of the pump unit and the amount of cancer cells (C) accommodated inside the single primary unit 700, and it is interlocked with the power unit and the pump unit to supply a medium or an inducing material. It may be provided to control the supply unit to supply.
즉, 본 발명의 또 다른 실시예에 따른 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치 및 이를 이용한 암세포의 형질전환 수집 관찰 시스템은 상기 관리자가 단말기를 통해서 배지 또는 유도물질의 흐름, 암세포(C)의 이동량을 측정하여 판단 및 관리할 수도 있다.That is, the transformed cancer cell collection device and the transformation collection and observation system of cancer cells using the same, according to another embodiment of the present invention, include the flow of the medium or the inducing material through the terminal, the cancer cells (C ) can be determined and managed by measuring the amount of movement.
앞서 설명된 실시예 이외에도 본 발명이 그 취지나 범주에서 벗어남이 없이 다른 특정 형태로 구체화될 수 있다는 사실은 해당 기술에 통상의 지식을 가진 이들에게는 자명한 것이다.In addition to the above-described embodiments, the fact that the present invention can be embodied in other specific forms without departing from the spirit or scope is apparent to those of ordinary skill in the art.
그러므로, 상술된 실시예는 제한적인 것이 아니라 예시적인 것으로 여겨져야 하고, 이에 따라 본 발명은 상술한 설명에 한정되지 않고 첨부된 청구항의 범주 및 그 동등 범위 내에서 변경될 수도 있다.Therefore, the above-described embodiments are to be regarded as illustrative rather than restrictive, and accordingly, the present invention is not limited to the above description, but may be modified within the scope of the appended claims and their equivalents.

Claims (45)

  1. 내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서,As a measurement unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium,
    외통과 상기 암세포가 수용되는 내통으로 구분되는 진원부;a genital part divided into an outer tube and an inner tube in which the cancer cells are accommodated;
    상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부; a supply unit communicating with the outer cylinder to provide a flow path for the medium to pass through the round unit;
    상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부; 및a moving unit coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells are moved; and
    상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 내통 내부를 부유하는 상기 암세포가 상기 이동부를 향해 이동되도록 유도물질을 분비하는 유도부를 포함하는, It is provided between the inner tube and the moving unit and includes an induction unit that secretes an inducer so that the cancer cells floating inside the inner tube are moved toward the moving unit by the flow of the medium.
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  2. 제1항에 있어서,According to claim 1,
    상기 유도부는,The induction part,
    이탈된 상기 암세포가 상기 이동부로 이동되도록 상기 암세포가 통과할 수 있는 다수의 막으로 형성되는 것을 특징으로 하는,characterized in that it is formed of a plurality of membranes through which the cancer cells can pass so that the separated cancer cells are moved to the moving unit,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  3. 제2항에 있어서,3. The method of claim 2,
    상기 유도부는,The induction part,
    상기 내통의 일면에 형성되는 제1 피막부재;a first film member formed on one surface of the inner cylinder;
    상기 제1 피막부재와 접촉되도록 구비되는 제2 피막부재; 및a second encapsulation member provided to be in contact with the first encapsulation member; and
    상기 제1 피막부재와 상기 제2 피막부재 사이에 구비되어 상기 암세포가 상기 이동부로 이동되도록 유도하며, 인체 내부의 혈관과 유사한 내피층을 형성하는 모조혈관내피부재를 포함하는 것을 특징으로 하는,It is provided between the first encapsulation member and the second encapsulation member to induce the cancer cells to move to the moving part, and comprises a imitation vascular inner skin material that forms an endothelial layer similar to blood vessels inside the human body,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  4. 제3항에 있어서,4. The method of claim 3,
    상기 모조혈관내피부재는,The imitation blood vessel inner skin material,
    상기 암세포가 통과할 수 있도록 혈관 내피세포로 형성되어 상기 제1 피막부재에 부착되고, 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 혈관내피층; 및a vascular endothelial layer formed of vascular endothelial cells to allow the cancer cells to pass through, attached to the first encapsulation member, and secreting an inducer to move the cancer cells to the moving part; and
    상기 혈관내피층과 상기 제2 피막부재 사이에 구비되어 상기 혈관내피층이 상기 제2 피막부재의 압력에 의해 손상되는 것을 방지하는 히드로겔층을 포함하는 것을 특징으로 하는,It characterized in that it comprises a hydrogel layer provided between the vascular endothelial layer and the second encapsulation member to prevent the vascular endothelial layer from being damaged by the pressure of the second encapsulation member,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  5. 제1항에 있어서,According to claim 1,
    상기 진원부는,The epicenter is
    상기 외통과 상기 내통이 소정거리 이격되도록 배치되는 것을 특징으로 하는, Characterized in that the outer cylinder and the inner cylinder are arranged to be spaced apart by a predetermined distance,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  6. 제5항에 있어서,6. The method of claim 5,
    상기 내통은,The inner pain is
    상기 공급부를 통해 상기 외통으로 유입되는 상기 배지가 내부로 유입되도록 상부를 향해 개구부가 형성되는 것을 특징으로 하는,Characterized in that an opening is formed toward the top so that the medium flowing into the outer cylinder through the supply unit flows into the inside,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  7. 제6항에 있어서,7. The method of claim 6,
    상기 내통은,The inner pain is
    상기 배지는 유입되고 상기 암세포는 상기 개구부로 이탈되는 것을 방지하도록 상기 개구부에 거름부재가 구비되는 것을 특징으로 하는,Characterized in that the medium is introduced and a filtering member is provided in the opening to prevent the cancer cells from escaping into the opening,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  8. 제1항에 있어서,According to claim 1,
    상기 이동부는,The moving unit,
    상기 암세포가 상기 이동부로 이동될 수 있도록 상기 유도부와 상기 외통 사이에서 상기 암세포의 이동을 가이드하는 가이드부재를 포함하는 것을 특징으로 하는, It characterized in that it comprises a guide member for guiding the movement of the cancer cells between the guide part and the outer cylinder so that the cancer cells can be moved to the moving part,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  9. 제8항에 있어서,9. The method of claim 8,
    상기 가이드부재는,The guide member,
    상기 유도부의 둘레에서 상기 이동부를 향해 직경이 좁아지도록 연장되는 것을 특징으로 하는,Characterized in that it extends from the periphery of the induction part toward the moving part so that the diameter becomes narrower,
    암세포의 형질전환을 유도하는 측정유닛.A measurement unit that induces transformation of cancer cells.
  10. 내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서, 외통과 암세포가 수용되는 내통으로 구분되는 진원부, 상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부, 상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부 및 상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 내통 내부를 부유하는 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 유도부를 포함하는 암세포의 형질전환을 유도하는 측정유닛; 및As a measuring unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium, a concave part divided into an outer tube and an inner tube in which cancer cells are accommodated, and a flow path in communication with the outer tube so that the medium passes through the epicenter A supply unit provided, a moving unit coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells are moved, and a cancer cell floating inside the inner cylinder provided between the inner cylinder and the moving unit and floating inside the inner cylinder by the flow of the medium is moved a measuring unit for inducing transformation of cancer cells including an inducing part that secretes an inducer to move to a part; and
    상기 이동부와 연통되고, 상기 이동부를 따라 이동되는 상기 암세포 및 상기 배지가 상기 진원부로 순환되도록 유로를 제공하는 순환유닛를 포함하는,and a circulation unit communicating with the moving unit and providing a flow path so that the cancer cells and the medium moving along the moving unit are circulated to the circular unit;
    순환식 암세포 형질전환 측정장치.Cyclic cancer cell transformation measuring device.
  11. 제10항에 있어서,11. The method of claim 10,
    상기 순환유닛은,The circulation unit is
    유로상에 구비되어 상기 이동부를 통해 이동되는 상기 암세포를 수집하는 수집부를 포함하는 것을 특징으로 하는,It is provided on the flow path, characterized in that it comprises a collecting unit for collecting the cancer cells moving through the moving unit,
    순환식 암세포 형질전환 측정장치.Cyclic cancer cell transformation measuring device.
  12. 제11항에 있어서,12. The method of claim 11,
    상기 수집부는,The collection unit,
    유로 일부를 대체하며, 상기 암세포가 추출되도록 다수의 막으로 형성되는 추출부재; 및an extraction member that replaces a part of the flow path and is formed of a plurality of membranes so that the cancer cells are extracted; and
    상기 추출부재 사이에 구비되어 상기 암세포의 이동을 촉진시키는 유도물질을 분비하고, 상기 암세포가 상기 추출부재를 통해 외부로 추출되는 것을 촉진시키는 촉진부재를 포함하는 것을 특징으로 하는,It is provided between the extraction members to secrete an inducer that promotes the movement of the cancer cells, characterized in that it comprises a promotion member that promotes the extraction of the cancer cells to the outside through the extraction member,
    순환식 암세포 형질전환 측정장치.Cyclic cancer cell transformation measuring device.
  13. 제10항에 있어서,11. The method of claim 10,
    상기 순환유닛은,The circulation unit is
    상기 배지 및 상기 암세포의 흐름이 적어도 하나 이상의 유로로 분할되는 서클부를 포함하는 것을 특징으로 하는,It characterized in that it comprises a circle part in which the flow of the medium and the cancer cells is divided into at least one flow path,
    순환식 암세포 형질전환 측정장치.Cyclic cancer cell transformation measuring device.
  14. 제13항에 있어서,14. The method of claim 13,
    상기 서클부는,The circle part,
    곡률반경을 가지며 절곡되어 분할된 유로가 통합되는 폐루프가 형성되는 것을 특징으로 하는,It has a radius of curvature and is bent to form a closed loop in which the divided flow paths are integrated,
    순환식 암세포 형질전환 측정장치.Cyclic cancer cell transformation measuring device.
  15. 제10항 내지 제14항 중 적어도 어느 한 항의 순환식 암세포 형질전환 측정장치를 이용한 암세포 형질전환 관찰 시스템으로서,15. A cancer cell transformation observation system using the circulating type cancer cell transformation measuring apparatus of at least one of claims 10 to 14,
    내피를 통과한 암세포의 형질전환을 관찰하기 위해 암세포의 형질전환을 유도하는 측정유닛으로서, 외통과 암세포가 수용되는 내통으로 구분되는 진원부, 상기 외통과 연통되어 배지가 상기 진원부를 통과하도록 유로를 제공하는 공급부, 상기 내통의 일면에 결합되어 상기 암세포가 이동되는 유로를 제공하는 이동부 및 상기 내통과 상기 이동부 사이에 구비되어 상기 배지의 흐름에 의해 상기 진원부 내부를 부유하는 상기 암세포가 상기 이동부로 이동되도록 유도물질을 분비하는 유도부를 포함하는 암세포의 형질전환을 유도하는 복수의 측정유닛;As a measuring unit for inducing transformation of cancer cells to observe transformation of cancer cells that have passed through the endothelium, a concave part divided into an outer tube and an inner tube in which cancer cells are accommodated, and a flow path in communication with the outer tube so that the medium passes through the epicenter A supply unit provided, a moving unit coupled to one surface of the inner cylinder to provide a flow path through which the cancer cells are moved, and a cancer cell that is provided between the inner tube and the moving unit and floats inside the round part by the flow of the medium is the a plurality of measurement units for inducing transformation of cancer cells including an inducing unit that secretes an inducer to move to a moving unit;
    상기 복수의 측정유닛과 연통되도록 유로가 분기되는 복수의 분기점이 형성되고, 상기 복수의 분기점이 서로 이어지도록 유로가 형성되어 상기 암세포 및 상기 배지가 순환되는 순환유닛을 포함하고, A plurality of bifurcation points are formed so as to communicate with the plurality of measurement units, and a circulation unit is formed through which the plurality of branch points are connected to each other so that the cancer cells and the medium are circulated,
    상기 복수의 측정유닛은, 상기 이동부가 상기 순환유닛을 향하도록 방사형으로 배치되는 것을 특징으로 하는, The plurality of measurement units, characterized in that the moving part is arranged radially to face the circulation unit,
    암세포 형질전환 관찰 시스템.Cancer cell transformation observation system.
  16. 제15항에 있어서,16. The method of claim 15,
    상기 순환유닛은,The circulation unit is
    유로상에 구비되어 상기 이동부를 통해 이동되는 상기 암세포를 수집하는 수집부를 포함하는 것을 특징으로 하는,It is provided on the flow path, characterized in that it comprises a collecting unit for collecting the cancer cells moving through the moving unit,
    암세포 형질전환 관찰 시스템.Cancer cell transformation observation system.
  17. 제16항에 있어서,17. The method of claim 16,
    상기 수집부는,The collection unit,
    유로의 일부를 대체하며, 상기 암세포가 추출되도록 다수의 막으로 형성되는 추출부재; 및 an extraction member that replaces a part of the flow path and is formed of a plurality of membranes so that the cancer cells are extracted; and
    상기 추출부재 사이에 구비되어 상기 암세포의 이동을 촉진시키는 유도물질을 배출하고, 상기 암세포가 상기 추출부재를 통해 외부로 추출되는 것을 촉진시키는 촉진부재를 포함하는 것을 특징으로 하는,It is provided between the extraction members to discharge the inducing material that promotes the movement of the cancer cells, characterized in that it comprises a facilitating member for accelerating the extraction of the cancer cells to the outside through the extraction member,
    암세포 형질전환 관찰 시스템.Cancer cell transformation observation system.
  18. 제15항에 있어서,16. The method of claim 15,
    상기 복수의 측정유닛은,The plurality of measurement units,
    상기 유도부에서 분비하는 유도물질 및 상기 암세포가 상기 순환유닛을 통해 서로 상호 교환되는 것을 특징으로 하는,characterized in that the inducer secreted by the induction unit and the cancer cells are mutually exchanged through the circulation unit,
    암세포 형질전환 관찰 시스템.Cancer cell transformation observation system.
  19. 제15항에 있어서,16. The method of claim 15,
    상기 순환유닛은,The circulation unit is
    일측에서 타측을 향해 곡률을 가지며 구부러져 일측과 타측이 서로 이어지는 폐루프를 가지는 서클부를 포함하는 것을 특징으로 하는,Characterized in that it includes a circle portion having a closed loop having a curvature from one side toward the other side and being bent so that one side and the other side are connected to each other,
    암세포의 형질전환 관찰 시스템.Cancer cell transformation observation system.
  20. 주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서,An inlet and outlet are formed, and in order to observe transformation of cancer cells passing through a blood vessel wall, a pseudovascular unit formed in a part of a path through which the medium and cancer cells move from the inlet to the outlet,
    경로상에 복수개가 이격되도록 경로를 따라 배치되어 상기 암세포가 외부로 유출되도록 이격된 틈을 형성하는 고리부;a ring portion disposed along the path so that a plurality of them are spaced apart on the path to form a spaced gap so that the cancer cells are discharged to the outside;
    내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 이격된 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 되는 피막부를 포함하는,A plurality of the ring portions are positioned therein, are formed of a plurality of membranes, and secrete an inducer to allow the cancer cells to flow out from the spaced gaps, and include a film portion that serves as a path through which the medium and the cancer cells move,
    모조혈관유닛.pseudovascular unit.
  21. 제20항에 있어서,21. The method of claim 20,
    상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 하는,A portion of the perimeter of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other,
    모조혈관유닛.pseudovascular unit.
  22. 제21항에 있어서,22. The method of claim 21,
    상기 연결부는,The connection part,
    상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 하는,Characterized in that the ring portion is formed to be elongated from the inlet toward the outlet so as to be fixed on the path and connects the plurality of the ring portions from the inside of the film portion,
    모조혈관유닛.pseudovascular unit.
  23. 제22항에 있어서,23. The method of claim 22,
    상기 연결부는,The connection part,
    상기 이격된 틈에서 인접한 상기 고리부를 연결하며 복수의 상기 고리부의 간격을 유지시키는 것을 특징으로 하는,Connecting the adjacent ring portions in the spaced gap, characterized in that maintaining the spacing of the plurality of ring portions,
    모조혈관유닛.pseudovascular unit.
  24. 제21항에 있어서,22. The method of claim 21,
    상기 연결부는,The connection part,
    상기 고리부의 둘레를 따라 다수개가 형성되는 것을 특징으로 하는,Characterized in that a plurality is formed along the circumference of the ring portion,
    모조혈관유닛.pseudovascular unit.
  25. 제22항에 있어서,23. The method of claim 22,
    상기 피막부는,The film portion,
    상기 고리부 및 상기 연결부를 감싸도록 구비되는 덮개부재;a cover member provided to surround the ring portion and the connection portion;
    상기 덮개부재의 둘레를 따라 형성되고, 상기 암세포가 외부로 이동되도록 유도물질을 분비하는 분비부재를 포함하는 것을 특징으로 하는,It is formed along the circumference of the cover member, characterized in that it comprises a secretion member that secretes an inducer to move the cancer cells to the outside,
    모조혈관유닛.pseudovascular unit.
  26. 제25항에 있어서,26. The method of claim 25,
    상기 분비부재는,The secretory member,
    혈관내피세포로 구성되는 것을 특징으로 하는,Characterized in that it is composed of vascular endothelial cells,
    모조혈관유닛.pseudovascular unit.
  27. 제25항에 있어서,26. The method of claim 25,
    상기 피막부는,The film portion,
    상기 분비부재의 수분이 유지되도록 상기 분비부재의 둘레를 따라 구비되는 수분유지부재를 더 포함하는 것을 특징으로 하는,Characterized in that it further comprises a moisture retention member provided along the circumference of the secretion member so that the moisture of the secretion member is maintained,
    모조혈관유닛.pseudovascular unit.
  28. 제27항에 있어서,28. The method of claim 27,
    상기 수분유지부재는,The moisture retention member,
    상기 고리부 사이에서 상기 분비부재의 둘레를 따라 형성되어 상기 분비부재를 감싸도록 형성되는 것을 특징으로 하는,It is formed along the circumference of the secretion member between the ring parts, characterized in that it is formed to surround the secretion member,
    모조혈관유닛.pseudovascular unit.
  29. 제20항에 있어서,21. The method of claim 20,
    상기 고리부는,The ring portion,
    상기 피막부의 내측 둘레와 접하며 상기 피막부를 지지하는 것을 특징으로 하는,It is in contact with the inner periphery of the coating portion, characterized in that supporting the coating portion,
    모조혈관유닛.pseudovascular unit.
  30. 주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서, 경로상에 복수개가 이격되도록 배치되어 상기 암세포가 외부로 유출되도록 틈을 형성하는 고리부, 내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 형성되는 피막부를 포함하는 모조혈관유닛;An inlet and outlet are formed, and in order to observe transformation of cancer cells passing through the blood vessel wall, a pseudovascular unit is formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet, so that a plurality of them are spaced apart on the path. A ring portion is disposed to form a gap so that the cancer cells are discharged to the outside, a plurality of the ring portions are positioned therein, and a plurality of membranes are formed to secrete an inducer so that the cancer cells are discharged to the outside from the gap, and the medium and a mimetic vascular unit comprising a membrane portion in which a path through which the cancer cells are moved is formed;
    내부에 공간이 형성되어 상기 암세포가 위치되고, 상기 주입구 및 상기 배출구와 연통되어 상기 배지가 순환되는 원발유닛; 및a primary unit in which a space is formed therein, the cancer cells are located, and the medium is circulated in communication with the inlet and the outlet; and
    상기 모조혈관유닛이 내부를 관통하며 배치되고, 상기 모조혈관유닛에서 외부로 이동되는 상기 암세포를 수집하는 수용유닛을 포함하는,The counterfeit blood vessel unit is disposed through the interior, and includes a receiving unit for collecting the cancer cells moving from the fake blood vessel unit to the outside,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  31. 제30항에 있어서,31. The method of claim 30,
    상기 수용유닛은,The receiving unit is
    상기 모조혈관유닛을 통해 이동하는 상기 암세포가 외부로 이동되는 것을 촉진하도록 유도물질이 수용되는 것을 특징으로 하는,Characterized in that the inducer is accommodated to promote the movement of the cancer cells moving through the pseudovascular unit to the outside,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  32. 제30항에 있어서,31. The method of claim 30,
    상기 원발유닛은,The primary unit is
    상기 주입구 및 상기 배출구와 연결되어 배지가 순환되는 순환부;a circulation unit connected to the inlet and the outlet to circulate the medium;
    상기 순환부와 연통되는 외부 하우징과 상기 외부 하우징과 이격되어 내부에 암세포가 위치되고, 상부를 향해 개구부가 형성되는 내부 하우징으로 구분되는 발상부;an idea part divided into an external housing communicating with the circulation part and an internal housing spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top;
    상기 배지의 순환에 의해 상기 내부 하우징을 부유하는 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 유도물질을 분비하며 상기 내부 하우징의 일면에 다수의 막으로 형성되는 촉진부를 포함하는 것을 특징으로 하는,Secreting an inducing material so that the cancer cells floating in the inner housing move toward the imitation vascular unit by circulation of the medium, characterized in that it comprises a facilitating part formed of a plurality of membranes on one surface of the inner housing,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  33. 제32항에 있어서,33. The method of claim 32,
    상기 내부 하우징은,The inner housing,
    상기 암세포가 외부로 이탈되는 것을 방지하기 위해 상기 개구부에 구비되는 거름부재를 포함하는 것을 특징으로 하는,It characterized in that it comprises a filtering member provided in the opening to prevent the cancer cells from escaping to the outside,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  34. 제32항에 있어서,33. The method of claim 32,
    상기 촉진부는,The promotion unit,
    상기 발상부의 일면에 형성되는 제1 상피부재;a first upper skin material formed on one surface of the idea part;
    상기 제1 상피부재와 접촉되도록 구비되는 제2 상피부재;a second upper skin member provided to be in contact with the first upper skin member;
    상기 제1 상피부재 및 상기 제2 상피부재 사이에 구비되어 유도물질을 분비하는 제3 상피부재를 포함하는 것을 특징으로 하는,It characterized in that it comprises a third epidermis that is provided between the first epidermis and the second epidermis to secrete an inducer,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  35. 제34항에 있어서,35. The method of claim 34,
    상기 제3 상피부재는,The third upper skin material,
    상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 혈관내피세포로 구비되어 제1 상피부재에 부착되는 모조피막층; 및a pseudocapsular layer provided with vascular endothelial cells and attached to the first epidermal material so that the cancer cells move toward the pseudovascular unit; and
    상기 제1 상피부재 및 상기 제2 상피부재에 의해 상기 모조피막층이 손상되는 것을 방지하기 위해 상기 모조피막층과 상기 제2 상피부재 사이에 구비되는 기질층을 포함하는 것을 특징으로 하는,In order to prevent the imitation coating layer from being damaged by the first epidermis and the second epidermis, it characterized in that it comprises a stromal layer provided between the imitation coat layer and the second epidermis,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  36. 제30항에 있어서,31. The method of claim 30,
    상기 모조혈관유닛은,The pseudovascular unit,
    상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 하는,A portion of the perimeter of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting each other,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  37. 제36항에 있어서,37. The method of claim 36,
    상기 연결부는,The connection part,
    상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 하는,Characterized in that the ring portion is formed to be elongated from the inlet toward the outlet so as to be fixed on the path and connects the plurality of the ring portions from the inside of the film portion,
    모조혈관유닛을 포함하는 형질전환된 암세포 수집장치.A transformed cancer cell collection device comprising a pseudovascular unit.
  38. 제30항 내지 제37항 중 적어도 어느 한 항의 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치를 이용한 암세포의 형질전환 수집 관찰 시스템으로서,38. A system for collecting and observing transformation of cancer cells using a transformed cancer cell collection device comprising the pseudovascular unit of any one of claims 30 to 37,
    주입구 및 배출구가 형성되고, 혈관벽을 통과하는 암세포의 형질전환을 관찰하기 위해 상기 주입구에서 상기 배출구를 향해 배지 및 암세포가 이동되는 경로의 일부에 형성되는 모조혈관유닛으로서, 경로상에 복수개가 이격되도록 배치되어 상기 암세포가 외부로 유출되도록 틈을 형성하는 고리부, 내부에 복수의 상기 고리부가 위치되고, 다수의 막으로 형성되어 상기 틈에서 상기 암세포가 외부로 유출되도록 유도물질을 분비하며, 상기 배지 및 상기 암세포가 이동되는 경로가 형성되는 피막부를 포함하는 모조혈관유닛, 내부에 공간이 형성되어 상기 암세포가 위치되고, 상기 주입구 및 상기 배출구와 연통되어 상기 배지가 순환되는 원발유닛 및 상기 모조혈관유닛이 내부를 관통하며 배치되고, 상기 모조혈관유닛에서 외부로 이동되는 상기 암세포를 수집하는 수용유닛을 포함하는 모조혈관유닛을 포함하는 형질전환된 암세포 수집장치; An inlet and outlet are formed, and in order to observe transformation of cancer cells passing through the blood vessel wall, a pseudovascular unit is formed in a part of the path through which the medium and cancer cells move from the inlet to the outlet, so that a plurality of them are spaced apart on the path. A ring portion is disposed to form a gap so that the cancer cells are discharged to the outside, a plurality of the ring portions are positioned therein, and a plurality of membranes are formed to secrete an inducer so that the cancer cells are discharged to the outside from the gap, and the medium and a imitation vascular unit comprising a membrane portion in which a path through which the cancer cells move is formed, a space is formed therein to place the cancer cells, and communicates with the inlet and the outlet to circulate the medium, and the imitation vascular unit a transformed cancer cell collecting device including a mimetic vascular unit disposed through the inside and including a receiving unit configured to collect the cancer cells moving from the mimetic vascular unit to the outside;
    유도물질이 상기 수용유닛을 통과하여 순환되도록 상기 수용유닛과 연통되는 순환장치; 및a circulation device communicating with the receiving unit so that the inducing material is circulated through the receiving unit; and
    상기 순환유닛의 경로상에 형성되며, 상기 수용유닛으로 이동된 상기 암세포가 유도물질의 흐름에 따라 이동되어 수집되는 추출장치를 포함하는,It is formed on the path of the circulation unit, and comprises an extraction device in which the cancer cells moved to the receiving unit are moved and collected according to the flow of the inducing material,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  39. 제38항에 있어서,39. The method of claim 38,
    상기 추출장치는,The extraction device is
    내부 공간이 단턱지게 형성되어 상기 암세포가 내부 공간에 침전되도록 하부에 공간이 형성되는 것을 특징으로 하는,Characterized in that the inner space is formed to be stepped, and a space is formed in the lower part so that the cancer cells are precipitated in the inner space,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  40. 제38항에 있어서,39. The method of claim 38,
    상기 순환장치는,The circulation device is
    유도물질의 이동경로상에 상기 유도물질이 이동되도록 동력을 제공하는 펌프부를 포함하는 것을 특징으로 하는,Characterized in that it comprises a pump unit for providing power to move the inducing material on the movement path of the inducing material,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  41. 제38항에 있어서,39. The method of claim 38,
    상기 원발장치는,The primary device is
    상기 주입구 및 상기 배출구와 연결되어 배지가 순환되는 순환부;a circulation unit connected to the inlet and the outlet to circulate the medium;
    상기 순환부와 연통되는 외부 하우징과 상기 외부 하우징과 이격되어 내부에 암세포가 위치되고, 상부를 향해 개구부가 형성되는 내부 하우징으로 구분되는 발상부;an idea part divided into an external housing communicating with the circulation part and an internal housing spaced apart from the external housing, the cancer cells are located therein, and an opening is formed toward the top;
    상기 배지의 순환에 의해 상기 내부 하우징을 부유하는 상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 유도물질을 분비하며 상기 내부 하우징의 일면에 다수의 막으로 형성되는 촉진부를 포함하는 것을 특징으로 하는,Secreting an inducing material so that the cancer cells floating in the inner housing move toward the imitation vascular unit by circulation of the medium, characterized in that it comprises a facilitating part formed of a plurality of membranes on one surface of the inner housing,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  42. 제41항에 있어서,42. The method of claim 41,
    상기 촉진부는,The promotion unit,
    상기 발상부의 일면에 형성되는 제1 상피부재;a first upper skin material formed on one surface of the idea part;
    상기 제1 상피부재와 접촉되도록 구비되는 제2 상피부재;a second upper skin member provided to be in contact with the first upper skin member;
    상기 제1 상피부재 및 상기 제2 상피부재 사이에 구비되어 유도물질을 분비하는 제3 상피부재를 포함하는 것을 특징으로 하는,It characterized in that it comprises a third epidermis that is provided between the first epidermis and the second epidermis to secrete an inducer,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  43. 제42항에 있어서,43. The method of claim 42,
    상기 제3 상피부재는,The third upper skin material,
    상기 암세포가 상기 모조혈관유닛을 향해 이동되도록 혈관내피세포로 구비되어 제1 상피부재에 부착되는 모조피막층; 및a pseudocapsular layer provided with vascular endothelial cells and attached to the first epidermal material so that the cancer cells move toward the pseudovascular unit; and
    상기 제1 상피부재 및 상기 제2 상피부재에 의해 상기 모조피막층이 손상되는 것을 방지하기 위해 상기 모조피막층과 상기 제2 상피부재 사이에 구비되는 기질층을 포함하는 것을 특징으로 하는,In order to prevent the imitation coating layer from being damaged by the first epidermis and the second epidermis, it characterized in that it comprises a stromal layer provided between the imitation coat layer and the second epidermis,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  44. 제38항에 있어서,39. The method of claim 38,
    상기 모조혈관유닛은,The pseudovascular unit,
    상기 고리부의 둘레 일부가 인접한 상기 고리부를 통해 연장되어 서로를 연결하는 연결부를 더 포함하는 것을 특징으로 하는,A portion of the perimeter of the ring portion is extended through the adjacent ring portion characterized in that it further comprises a connecting portion for connecting to each other,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
  45. 제44항에 있어서,45. The method of claim 44,
    상기 연결부는,The connection part,
    상기 고리부가 경로상에 고정되도록 상기 주입구에서 상기 배출구를 향해 길게 형성되어 상기 피막부 내측에서 복수의 상기 고리부를 연결하는 것을 특징으로 하는, Characterized in that the ring portion is formed to be elongated from the inlet toward the outlet so as to be fixed on the path and connects the plurality of the ring portions from the inside of the film portion,
    암세포의 형질전환 수집 관찰 시스템.Transformation collection observation system of cancer cells.
PCT/KR2021/095077 2020-08-18 2021-08-10 Cancer cell transformation observation system using circulation-type cancer cell transformation measuring device and device for collecting transformed cancer cells comprising artificial blood vessel unit WO2022039584A1 (en)

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KR1020200103130A KR102502401B1 (en) 2020-08-18 2020-08-18 A transfromed cancer cell cellection device comprising a mock vessel unit and a cancer cell transformation observation system using the same
KR1020200103122A KR102420392B1 (en) 2020-08-18 2020-08-18 A circulating cancer cell transformation measuring apparatus including a measuring unit for induing transformation of cancer cell and a cancer cell transformation observation system using the same
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