WO2022012481A1 - Crystalline or Amorphous form of Bcl-2/Bcl-xL inhibitor compound or its salts - Google Patents
Crystalline or Amorphous form of Bcl-2/Bcl-xL inhibitor compound or its salts Download PDFInfo
- Publication number
- WO2022012481A1 WO2022012481A1 PCT/CN2021/105856 CN2021105856W WO2022012481A1 WO 2022012481 A1 WO2022012481 A1 WO 2022012481A1 CN 2021105856 W CN2021105856 W CN 2021105856W WO 2022012481 A1 WO2022012481 A1 WO 2022012481A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- formula
- compound
- basically
- chart
- mdsc
- Prior art date
Links
- 150000001875 compounds Chemical class 0.000 title claims abstract description 198
- 150000003839 salts Chemical class 0.000 title claims abstract description 30
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 title claims abstract description 18
- 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 title claims abstract description 18
- 229940122035 Bcl-XL inhibitor Drugs 0.000 title abstract description 7
- 229940123711 Bcl2 inhibitor Drugs 0.000 title abstract description 7
- 238000000634 powder X-ray diffraction Methods 0.000 claims description 77
- 238000001565 modulated differential scanning calorimetry Methods 0.000 claims description 59
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 51
- 239000002904 solvent Substances 0.000 claims description 49
- 239000007787 solid Substances 0.000 claims description 47
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 42
- 238000003756 stirring Methods 0.000 claims description 35
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 32
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical class OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 32
- 238000002411 thermogravimetry Methods 0.000 claims description 31
- 238000010586 diagram Methods 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 29
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 28
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 20
- 206010028980 Neoplasm Diseases 0.000 claims description 20
- 230000009477 glass transition Effects 0.000 claims description 20
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical class CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 claims description 19
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 18
- 235000001968 nicotinic acid Nutrition 0.000 claims description 17
- 239000011664 nicotinic acid Substances 0.000 claims description 17
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 15
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 claims description 15
- 239000012296 anti-solvent Substances 0.000 claims description 15
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 15
- 159000000000 sodium salts Chemical class 0.000 claims description 15
- 239000002253 acid Substances 0.000 claims description 14
- 201000011510 cancer Diseases 0.000 claims description 14
- 239000012046 mixed solvent Substances 0.000 claims description 12
- 230000004580 weight loss Effects 0.000 claims description 12
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 11
- 102100026596 Bcl-2-like protein 1 Human genes 0.000 claims description 10
- 239000001530 fumaric acid Substances 0.000 claims description 10
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-dimethylformamide Substances CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 8
- 238000000113 differential scanning calorimetry Methods 0.000 claims description 8
- 150000002688 maleic acid derivatives Chemical class 0.000 claims description 8
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 6
- QCQCHGYLTSGIGX-GHXANHINSA-N 4-[[(3ar,5ar,5br,7ar,9s,11ar,11br,13as)-5a,5b,8,8,11a-pentamethyl-3a-[(5-methylpyridine-3-carbonyl)amino]-2-oxo-1-propan-2-yl-4,5,6,7,7a,9,10,11,11b,12,13,13a-dodecahydro-3h-cyclopenta[a]chrysen-9-yl]oxy]-2,2-dimethyl-4-oxobutanoic acid Chemical compound N([C@@]12CC[C@@]3(C)[C@]4(C)CC[C@H]5C(C)(C)[C@@H](OC(=O)CC(C)(C)C(O)=O)CC[C@]5(C)[C@H]4CC[C@@H]3C1=C(C(C2)=O)C(C)C)C(=O)C1=CN=CC(C)=C1 QCQCHGYLTSGIGX-GHXANHINSA-N 0.000 claims description 6
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical class OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 claims description 6
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 6
- 229960003512 nicotinic acid Drugs 0.000 claims description 6
- 239000008194 pharmaceutical composition Substances 0.000 claims description 6
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000011976 maleic acid Substances 0.000 claims description 5
- 229960003194 meglumine Drugs 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 4
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 claims description 4
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims description 4
- 230000008901 benefit Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 150000002148 esters Chemical class 0.000 claims description 4
- 150000008282 halocarbons Chemical class 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 150000002825 nitriles Chemical class 0.000 claims description 4
- 239000003880 polar aprotic solvent Substances 0.000 claims description 4
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- JMMWKPVZQRWMSS-UHFFFAOYSA-N isopropyl acetate Chemical compound CC(C)OC(C)=O JMMWKPVZQRWMSS-UHFFFAOYSA-N 0.000 claims description 3
- 229940116298 l- malic acid Drugs 0.000 claims description 3
- 235000011090 malic acid Nutrition 0.000 claims description 3
- 238000011282 treatment Methods 0.000 claims description 3
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 claims description 2
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 claims 3
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 claims 2
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 claims 2
- 206010025323 Lymphomas Diseases 0.000 claims 2
- 206010029260 Neuroblastoma Diseases 0.000 claims 2
- 208000006265 Renal cell carcinoma Diseases 0.000 claims 2
- 201000005787 hematologic cancer Diseases 0.000 claims 2
- 208000024200 hematopoietic and lymphoid system neoplasm Diseases 0.000 claims 2
- 208000025113 myeloid leukemia Diseases 0.000 claims 2
- 208000031261 Acute myeloid leukaemia Diseases 0.000 claims 1
- 208000036762 Acute promyelocytic leukaemia Diseases 0.000 claims 1
- 206010003571 Astrocytoma Diseases 0.000 claims 1
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 claims 1
- 206010005003 Bladder cancer Diseases 0.000 claims 1
- 206010006187 Breast cancer Diseases 0.000 claims 1
- 208000026310 Breast neoplasm Diseases 0.000 claims 1
- 206010008342 Cervix carcinoma Diseases 0.000 claims 1
- 208000010833 Chronic myeloid leukaemia Diseases 0.000 claims 1
- 206010009944 Colon cancer Diseases 0.000 claims 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 claims 1
- 201000008808 Fibrosarcoma Diseases 0.000 claims 1
- 208000022072 Gallbladder Neoplasms Diseases 0.000 claims 1
- 208000032612 Glial tumor Diseases 0.000 claims 1
- 206010018338 Glioma Diseases 0.000 claims 1
- 208000017604 Hodgkin disease Diseases 0.000 claims 1
- 208000021519 Hodgkin lymphoma Diseases 0.000 claims 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 claims 1
- 206010023347 Keratoacanthoma Diseases 0.000 claims 1
- 208000008839 Kidney Neoplasms Diseases 0.000 claims 1
- 208000031671 Large B-Cell Diffuse Lymphoma Diseases 0.000 claims 1
- 206010023825 Laryngeal cancer Diseases 0.000 claims 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims 1
- 201000003793 Myelodysplastic syndrome Diseases 0.000 claims 1
- 208000033761 Myelogenous Chronic BCR-ABL Positive Leukemia Diseases 0.000 claims 1
- 208000033776 Myeloid Acute Leukemia Diseases 0.000 claims 1
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 claims 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 claims 1
- 206010033128 Ovarian cancer Diseases 0.000 claims 1
- 206010061535 Ovarian neoplasm Diseases 0.000 claims 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 claims 1
- 206010035226 Plasma cell myeloma Diseases 0.000 claims 1
- 206010060862 Prostate cancer Diseases 0.000 claims 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims 1
- 208000015634 Rectal Neoplasms Diseases 0.000 claims 1
- 206010038389 Renal cancer Diseases 0.000 claims 1
- 201000010208 Seminoma Diseases 0.000 claims 1
- 208000000453 Skin Neoplasms Diseases 0.000 claims 1
- 208000005718 Stomach Neoplasms Diseases 0.000 claims 1
- 206010042971 T-cell lymphoma Diseases 0.000 claims 1
- 208000027585 T-cell non-Hodgkin lymphoma Diseases 0.000 claims 1
- 206010043276 Teratoma Diseases 0.000 claims 1
- 208000024313 Testicular Neoplasms Diseases 0.000 claims 1
- 206010057644 Testis cancer Diseases 0.000 claims 1
- 208000024770 Thyroid neoplasm Diseases 0.000 claims 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 claims 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 claims 1
- 201000006083 Xeroderma Pigmentosum Diseases 0.000 claims 1
- 230000001154 acute effect Effects 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 210000001185 bone marrow Anatomy 0.000 claims 1
- 210000003169 central nervous system Anatomy 0.000 claims 1
- 201000010881 cervical cancer Diseases 0.000 claims 1
- 208000029742 colonic neoplasm Diseases 0.000 claims 1
- 201000004101 esophageal cancer Diseases 0.000 claims 1
- 201000010175 gallbladder cancer Diseases 0.000 claims 1
- 206010017758 gastric cancer Diseases 0.000 claims 1
- 208000005017 glioblastoma Diseases 0.000 claims 1
- 210000002768 hair cell Anatomy 0.000 claims 1
- 201000010982 kidney cancer Diseases 0.000 claims 1
- 206010023841 laryngeal neoplasm Diseases 0.000 claims 1
- 208000032839 leukemia Diseases 0.000 claims 1
- 201000007270 liver cancer Diseases 0.000 claims 1
- 208000014018 liver neoplasm Diseases 0.000 claims 1
- 201000005202 lung cancer Diseases 0.000 claims 1
- 208000020816 lung neoplasm Diseases 0.000 claims 1
- 210000002751 lymph Anatomy 0.000 claims 1
- 210000004324 lymphatic system Anatomy 0.000 claims 1
- 201000007275 lymphatic system cancer Diseases 0.000 claims 1
- 208000003747 lymphoid leukemia Diseases 0.000 claims 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 claims 1
- 201000001441 melanoma Diseases 0.000 claims 1
- 201000000050 myeloid neoplasm Diseases 0.000 claims 1
- 208000007538 neurilemmoma Diseases 0.000 claims 1
- 201000008968 osteosarcoma Diseases 0.000 claims 1
- 201000002528 pancreatic cancer Diseases 0.000 claims 1
- 208000008443 pancreatic carcinoma Diseases 0.000 claims 1
- 210000001428 peripheral nervous system Anatomy 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 206010038038 rectal cancer Diseases 0.000 claims 1
- 201000001275 rectum cancer Diseases 0.000 claims 1
- 201000006845 reticulosarcoma Diseases 0.000 claims 1
- 208000029922 reticulum cell sarcoma Diseases 0.000 claims 1
- 201000009410 rhabdomyosarcoma Diseases 0.000 claims 1
- 206010039667 schwannoma Diseases 0.000 claims 1
- 201000000849 skin cancer Diseases 0.000 claims 1
- 201000011549 stomach cancer Diseases 0.000 claims 1
- 208000011580 syndromic disease Diseases 0.000 claims 1
- 201000003120 testicular cancer Diseases 0.000 claims 1
- 201000002510 thyroid cancer Diseases 0.000 claims 1
- 208000030901 thyroid gland follicular carcinoma Diseases 0.000 claims 1
- 201000005112 urinary bladder cancer Diseases 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 36
- 239000000243 solution Substances 0.000 description 41
- 239000011521 glass Substances 0.000 description 34
- 239000000523 sample Substances 0.000 description 29
- 238000012360 testing method Methods 0.000 description 26
- -1 Bcl-xL Proteins 0.000 description 15
- 239000000725 suspension Substances 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 12
- 230000006907 apoptotic process Effects 0.000 description 10
- 238000001556 precipitation Methods 0.000 description 9
- 108010090931 Proto-Oncogene Proteins c-bcl-2 Proteins 0.000 description 8
- 102000013535 Proto-Oncogene Proteins c-bcl-2 Human genes 0.000 description 8
- 239000002585 base Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 235000019439 ethyl acetate Nutrition 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 239000013078 crystal Substances 0.000 description 7
- 238000002425 crystallisation Methods 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 239000002244 precipitate Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000001291 vacuum drying Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- 108700000711 bcl-X Proteins 0.000 description 3
- 102000055104 bcl-X Human genes 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 238000009509 drug development Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003760 magnetic stirring Methods 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000000861 pro-apoptotic effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000002424 anti-apoptotic effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000012395 formulation development Methods 0.000 description 2
- 238000005734 heterodimerization reaction Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- NSETWVJZUWGCKE-UHFFFAOYSA-N propylphosphonic acid Chemical compound CCCP(O)(O)=O NSETWVJZUWGCKE-UHFFFAOYSA-N 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000010583 slow cooling Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000011269 treatment regimen Methods 0.000 description 2
- JXYCCKVJTSXZRI-UHFFFAOYSA-N 1,4-dioxane;heptane Chemical compound C1COCCO1.CCCCCCC JXYCCKVJTSXZRI-UHFFFAOYSA-N 0.000 description 1
- JWUJQDFVADABEY-UHFFFAOYSA-N 2-methyltetrahydrofuran Chemical compound CC1CCCO1 JWUJQDFVADABEY-UHFFFAOYSA-N 0.000 description 1
- 102000010565 Apoptosis Regulatory Proteins Human genes 0.000 description 1
- 108010063104 Apoptosis Regulatory Proteins Proteins 0.000 description 1
- 101001056180 Homo sapiens Induced myeloid leukemia cell differentiation protein Mcl-1 Proteins 0.000 description 1
- 102100026539 Induced myeloid leukemia cell differentiation protein Mcl-1 Human genes 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- WNLRTRBMVRJNCN-UHFFFAOYSA-L adipate(2-) Chemical compound [O-]C(=O)CCCCC([O-])=O WNLRTRBMVRJNCN-UHFFFAOYSA-L 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 102000055574 bcl-2 Homologous Antagonist-Killer Human genes 0.000 description 1
- 108700039689 bcl-2 Homologous Antagonist-Killer Proteins 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000005880 cancer cell killing Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005496 eutectics Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000012803 optimization experiment Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 238000010837 poor prognosis Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- IWZKICVEHNUQTL-UHFFFAOYSA-M potassium hydrogen phthalate Chemical compound [K+].OC(=O)C1=CC=CC=C1C([O-])=O IWZKICVEHNUQTL-UHFFFAOYSA-M 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000012453 solvate Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000030968 tissue homeostasis Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
- C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/675—Phosphorus compounds having nitrogen as a ring hetero atom, e.g. pyridoxal phosphate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C213/00—Preparation of compounds containing amino and hydroxy, amino and etherified hydroxy or amino and esterified hydroxy groups bound to the same carbon skeleton
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C215/00—Compounds containing amino and hydroxy groups bound to the same carbon skeleton
- C07C215/02—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton
- C07C215/04—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated
- C07C215/06—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic
- C07C215/10—Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic with one amino group and at least two hydroxy groups bound to the carbon skeleton
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/41—Preparation of salts of carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/41—Preparation of salts of carboxylic acids
- C07C51/412—Preparation of salts of carboxylic acids by conversion of the acids, their salts, esters or anhydrides with the same carboxylic acid part
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C55/00—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms
- C07C55/02—Dicarboxylic acids
- C07C55/14—Adipic acid
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
- C07C57/02—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms with only carbon-to-carbon double bonds as unsaturation
- C07C57/13—Dicarboxylic acids
- C07C57/145—Maleic acid
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
- C07C57/02—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms with only carbon-to-carbon double bonds as unsaturation
- C07C57/13—Dicarboxylic acids
- C07C57/15—Fumaric acid
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
- C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
- C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
- C07D213/79—Acids; Esters
- C07D213/80—Acids; Esters in position 3
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
- C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
- C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
- C07D213/79—Acids; Esters
- C07D213/803—Processes of preparation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/13—Crystalline forms, e.g. polymorphs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
- C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
- C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
Definitions
- the invention belongs to the field of medicinal chemistry, and particularly relates to a crystalline or amorphous form of a Bcl-2/Bcl-xL inhibitor compound or a salt thereof, and a preparation method and application thereof.
- Apoptosis is the process of programmed cell death and an essential biological process for tissue homeostasis. Apoptosis resistance is a hallmark of human cancer.
- One of the main ways for cancer cells to evade apoptosis is through up-regulation of anti-apoptotic Bcl-2 family proteins.
- Targeting key apoptosis regulators to overcome apoptosis resistance and promote tumor cell apoptosis is a new cancer treatment strategy.
- the Bcl-2 protein functions as a key regulator of apoptosis in both cancer cells and normal cells.
- the Bcl-2 protein serves as a check for apoptosis, allowing healthy and useful cells to survive.
- This protein family includes anti-apoptotic proteins, such as Bcl-2, Bcl-xL, and Mcl-1, and pro-apoptotic molecules, including Bid, Bim, Bad, Bak, and Bax.
- anti-apoptotic proteins such as Bcl-2, Bcl-xL, and Mcl-1
- pro-apoptotic molecules including Bid, Bim, Bad, Bak, and Bax.
- normal cells have low expression levels of anti-apoptotic Bcl-2 and Bcl-xL proteins, these proteins have been found to be highly overexpressed in many different types of human tumors. These overexpressions are related to the poor prognosis of several types of cancer, and are related to clinical resistance to chemotherapeutics and radiation.
- Bcl-2 or Bcl-xL causes cancer cells to become more resistant to chemotherapeutic agents in vitro and in vivo. Therefore, it has been sought to target Bcl-2 and/or Bcl-xL as a cancer treatment strategy. Inhibiting the Bcl-2 activity of cancer cells can reduce chemotherapy resistance and increase cancer cell killing.
- Bcl-2 and Bcl-xL proteins inhibit apoptosis by heterodimerization with pro-apoptotic Bcl-2 family proteins such as Bak, Bax, Bim, Bid, Puma, and Bad.
- pro-apoptotic Bcl-2 family proteins such as Bak, Bax, Bim, Bid, Puma, and Bad.
- the experimentally determined three-dimensional structures of Bcl-xL and Bcl-2 have shown that these proteins have well-defined grooves that interact with the BH3 domain of the pro-apoptotic Bcl-2 protein.
- a non-peptide small molecule designed to block the heterodimerization of the Bcl-2/Bcl-xL protein and its pro-death binding partner can be effective as an antagonist of Bcl-2/Bcl-xL, and the small Molecular inhibitors may have great therapeutic potential to treat human cancers in which Bcl-2 and/or Bcl-xL are highly expressed.
- WO2014/113413A1 discloses a small molecule Bcl-2/Bcl-xL inhibitor for inducing apoptosis of cancer cells expressing Bcl-2 and/or Bcl-xL, and specifically discloses representative compounds: (R) -3- (1- (3- (4- (N- (4- (4- (3- (2- (4-chlorophenyl) -1-isopropyl-5-methyl-4- (methylsulfonyl) -1H-pyrrol-3-yl) -5-fluorophenyl) piperazin-1-yl) phenyl) sulfamoyl) -2- (trifluoromethylsulfonyl) phenylamino) -4- (phenylthio) buty l) piperidine-4-carbonyloxy) propylphosphonic acid (Hereinafter referred to as the compound of formula 1) , Its structural formula is as follows:
- some solid compounds may also have Amorphous forms.
- Amorphous refers to the structure of some non-completely crystalline Amorphous regions (amorphous regions) or the formation of some Amorphous solids (amorphous) .
- amorphous the existence and quantity of its Amorphous form are also unpredictable, and may also have a significant impact on the solubility, melting point, density, and stability of the drug. Therefore, in the process of new drug development, a comprehensive screening of crystalline and amorphous forms of drug compounds is required, considering multiple factors.
- the invention provides a crystalline form or amorphous form of a Bcl-2/Bcl-xL inhibitor compound or a salt thereof, and a preparation method and application thereof.
- the crystalline form or Amorphous form of the present invention has very important value for drug development, formulation development and production.
- the present invention provides a crystalline or Amorphous form of the compound of formula 1 or its salts:
- the chemical name of the compound is (R) -3- (1- (3- (4- (N- (4- (4- (3- (2- (4-chlorophenyl) -1-isopropyl-5-methyl-4- (methylsulfonyl) -1H-pyrrol-3-yl) -5-fluorophenyl) piperazin-1-yl) phenyl) sulfamoyl) -2- (trifluoromethylsulfonyl) phenylamino) -4- (phenylthio) buty l) piperidine-4-carbonyloxy) propylphosphonic acid.
- the form may be the following specific forms:
- the form is the crystalline form I of the fumarate salt of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in an X-ray powder diffraction (XRPD) pattern expressed by an angle of 2 ⁇ : 18.03 ⁇ 0.2°, 28.81 ⁇ 0.2° and 31.88 ⁇ 0.2°.
- XRPD X-ray powder diffraction
- the form has XRPD characteristic peaks at positions basically as shown in Table 1 below and/or an X-ray powder diffraction (XRPD) pattern basically as shown in FIG. 1.
- XRPD X-ray powder diffraction
- the form also has the following characteristics:
- the form is the crystalline form II of the hydrochloride salt of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2 ⁇ : 13.01 ⁇ 0.2°, 21.55 ⁇ 0.2°, 25.95 ⁇ 0.2°, 31.33 ⁇ 0.2° and 37.34 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 2 below and/or an XRPD pattern basically as shown in FIG. 3.
- the form also has the following characteristics:
- the form is the nicotinic acid salt crystalline form III of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram expressed at 2 ⁇ angles: 15.46 ⁇ 0.2° and 18.06 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 3 below and/or an XRPD pattern basically as shown in FIG. 5.
- the form also has the following characteristics:
- the form is the sodium salt crystalline form IV of the compound of formula 1, which is characterized in that it has at least three or four characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2 ⁇ : 16.75 ⁇ 0.2°, 32.34 ⁇ 0.2°, 33.48 ⁇ 0.2° and 37.95 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 4 below and/or an XRPD pattern basically as shown in FIG. 7.
- the form is the crystalline form V of the potassium salt of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2 ⁇ : 24.35 ⁇ 0.2°, 30.10 ⁇ 0.2° and 31.41 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 5 below and/or an XRPD pattern basically as shown in FIG. 8.
- the form is the crystalline form VI of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2 ⁇ : 3.94 ⁇ 0.2°, 5.09 ⁇ 0.2°, 18.01 ⁇ 0.2°, 19.08 ⁇ 0.2°, 19.70 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 6 below and/or an XRPD pattern basically as shown in FIG. 9.
- the form also has the following characteristics:
- the form is the crystalline form VII of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2 ⁇ : 20.18 ⁇ 0.2°, 20.74 ⁇ 0.2°, 29.20 ⁇ 0.2°, 29.28 ⁇ 0.2° and 36.73 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 7 below and/or an XRPD pattern basically as shown in FIG. 12.
- the form also has the following characteristics:
- the form is the crystalline form VIII of the compound of formula 1, which is characterized in that it has a characteristic peak at the following position in the XRPD diagram represented by an angle of 2 ⁇ : 18.11 ⁇ 0.2°.
- the form has XRPD characteristic peaks at positions basically as shown in Table 8 below and/or an XRPD pattern basically as shown in FIG. 14.
- the form also has the following characteristics:
- the form is the Amorphous Form IX of the adipate salt of the compound of Formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 17.
- the form also has the following characteristics:
- the form is the fumarate Amorphous form X of the compound of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 19.
- the form also has the following characteristics:
- the form is the Amorphous form XI of the compound nicotinate of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 21.
- the form also has the following characteristics:
- the form is the amorphous form XII of the potassium salt of the compound of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 23.
- the form also has the following characteristics:
- the form is the amorphous Form XIII of the sodium salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 25.
- the form also has the following characteristics:
- the form is the amorphous form XIV of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 27.
- the form also has the following characteristics:
- the form is the amorphous form XV of the maleate salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 30.
- the form also has the following characteristics:
- the form is the amorphous form XVI of the meglumine salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 33.
- the form also has the following characteristics:
- the present invention provides a method for preparing the crystalline or amorphous form of the compound of formula 1 or its salt.
- the present invention provides a method for preparing the crystalline form of the salt of the compound of formula 1, which comprises the following steps: mixing the compound of formula 1 with a solvent and an acid or base, and slowly volatilizing the resulting solution at room temperature to obtain the formula 1 The crystalline form of the salt of the compound.
- the solvent can be a solvent commonly used in laboratories, such as one or more of water, alkane, alcoho, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbon solvents and polar aprotic solvents, such as DMF and DMSO.
- the mass-volume ratio of the compound of formula 1 to the solvent can be 100 mg: (0.1-10 mL) .
- the acid may be a pharmaceutically acceptable acid or an acid commonly used in the art, and may be an inorganic acid or an organic acid. Further preferably, the acid may be fumaric acid, hydrochloric acid, niacin, maleic acid and the like.
- the base may be a pharmaceutically acceptable base or a base commonly used in the art. Further preferably, the alkali may be sodium hydroxide, potassium hydroxide, meglumine and the like.
- the present invention provides the preparation of the crystalline form VI of the compound of formula 1, which includes the following steps: dissolving the compound of formula 1 in acetone, then adding methanol and stirring, and separating the resulting solid to obtain crystalline form VI of the compound of formula 1.
- the present invention provides the preparation of crystalline form VII of the compound of formula 1, which comprises the following steps: dissolving the compound of formula 1 in ethyl acetate, then adding L-malic acid and stirring, and separating the resulting solid to obtain crystalline form VII of the compound of formula 1.
- the present invention provides the preparation of the crystalline form VIII of the compound of formula 1, which includes the following steps: adding the compound of formula 1 to a tetrahydrofuran/n-hexane mixed solvent, and after stirring, the resulting solid is separated to obtain crystalline form VIII of the compound of formula 1.
- the present invention provides a method for preparing the Amorphous form of the salt of the compound of formula 1, which comprises the following steps: mixing the compound of formula 1 with a solvent and an acid or base, and separating the resulting solid to obtain Amorphous form of the salt of the compound of formula 1
- the solvent can be a solvent commonly used in laboratories, such as: One or more of water, alkane, alcohol, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbon solvents and polar aprotic solvents, such as DMF and DMSO.
- the mass-volume ratio of the compound of formula 1 to the solvent can be 100 mg: (0.1-10 mL) .
- the acid may be a pharmaceutically acceptable acid or an acid commonly used in the art, and may be an inorganic acid or an organic acid. Further preferably, the acid may be fumaric acid, hydrochloric acid, niacin, maleic acid and the like.
- the base may be a pharmaceutically acceptable base or a base commonly used in the art. Further preferably, the alkali may be sodium hydroxide, potassium hydroxide, meglumine and the like.
- the present invention provides a method for preparing the Amorphous form XIV of the compound of formula 1, which comprises the following steps: adding the compound of formula 1 to a solvent to obtain a solution, then adding the resulting solution to an anti-solvent, and separating the obtained solid to obtain the Amorphous form XIV of the compound of formula 1.
- the solvent is selected from one or more of ethyl acetate, acetone, tetrahydrofuran, acetonitrile, DMSO, 1, 4-dioxane, MIBK, dichloromethane, and DMF; and/or the
- the anti-solvent is selected from one or more of heptane, ethanol, water, IPAc, IPA, butanol, and NPA.
- the preparation temperature can be a temperature that can be conventional in the art, for example, 20-50°C.
- the crystallization time is not particularly limited, as long as the crystals can be precipitated, for example, the crystallization time can be 1-48h.
- the preparation method of the crystalline form or the amorphous form of the compound of formula 1 or its salt may also be a method well known in the art, such as solvent volatilization method, suspension stirring method, heating and cooling crystallization method, and mixed solvent crystallization method.
- the solvent volatilization method of the present invention is to volatilize the clear solution of the sample at different temperatures until the solvent is volatilized to dryness.
- the suspension stirring method of the present invention is to stir the supersaturated solution of the sample (with undissolved solids) in different solvents for a period of time.
- the heating and cooling crystallization method of the present invention is to dissolve the sample in an appropriate solvent under high temperature conditions, and after filtering, the filtrate is stirred and precipitated in a room temperature or low temperature environment.
- the mixed solvent crystallization method of the present invention is to take a sample and dissolve it in an appropriate solvent, add another or more solvents, and precipitate a solid system for a short time after stirring and filtering.
- the present invention provides a pharmaceutical composition
- a pharmaceutical composition comprising the crystalline or amorphous form of the compound of formula 1 or its salt, and pharmaceutically acceptable excipients.
- the compound of formula 1 or its salt, crystalline form or amorphous form of a solvate may be a therapeutically effective amount.
- the pharmaceutically acceptable excipients may be well-known excipients in the art. In the case of solid preparations, they include, but are not limited to: diluents, binders, disintegrants, lubricants, glidants, release rate control agents, plasticizers, preservatives, antioxidants, etc.
- the pharmaceutical composition can be selected in a dosage form suitable for human consumption, such as: tablets, capsules, granules, powdersor pills, etc., preferably tablets, capsules, granules, disintegrating tablets, sustained-release or controlled-release tablets etc.
- the pharmaceutical composition of the present invention can be prepared by various methods well known in the art, and a therapeutically effective amount of one or more of the crystalline form or the amorphous form of the compound of formula 1 or its salt can be combined with one or more a variety of pharmaceutically acceptable excipients are mixed to prepare a dosage form suitable for human consumption, such as tablets, capsules, and granules.
- “Therapeutically effective amount” refers to the amount of the compound form according to the present invention, which when administered to a patient in need thereof, is sufficient to achieve the treatment of a disease state, condition or disorder for which the compound is effective. Such an amount will be sufficient to elicit the biological or medical response of the tissue system or patient sought by the researcher or clinician.
- the present invention provides a crystalline form or Amorphous form of the compound of the above formula 1 or its salt or the above pharmaceutical composition for use in the treatment or prevention of diseases in which the inhibition of Bcl-2 or Bcl-xL provides benefits in medicine.
- the disease in which the inhibition of Bcl-2 or Bcl-xL provides benefit is cancer.
- the crystalline form or amorphous form of the compound of formula 1 or its salt of the present invention has the following advantages:
- Various forms of the present invention including the amorphous form XIV of the compound of formula 1, the meglumine salt amorphous form XVI and the crystalline form VI of the compound of formula 1 have good physical and chemical stability.
- the present invention improves the preparation method of the crystalline form VI of the compound of formula 1, thereby improving the purity of the product.
- Figure 1 is the XPRD pattern of the crystalline form I of the fumarate salt of formula 1.
- Figure 2 is the MDSC chart of the crystalline form I of the fumarate salt of formula 1.
- Figure 3 is the XPRD pattern of the crystalline form II of the hydrochloride salt of formula 1.
- Figure 4 is the MDSC chart of the crystalline form II of the hydrochloride salt of formula 1.
- Figure 5 is the XPRD pattern of the crystalline form III of the Nicotinate of formula 1.
- Figure 6 is the MDSC chart of the crystalline form III of the Nicotinateof formula 1.
- Figure 7 is the XPRD pattern of the crystalline form IV of the sodium salt of formula 1.
- Figure 8 is the XPRD pattern of the crystalline form V of the potassium salt of formula1.
- Figure 9 is the XPRD pattern of the crystalline form VI of formula 1.
- Figure 10 is the TGA diagram of the crystalline form VI of formula 1.
- Figure 11 is a DSC chart of the crystalline form VI of formula 1.
- Figure 12 is the XPRD pattern of the crystalline form VII of formula 1.
- Figure 13 is a DSC chart of the crystalline form VII of formula 1.
- Figure 14 is the XPRD pattern of the crystalline form VIII of formula 1.
- Figure 15 is the TGA diagram of the crystalline form VIII of formula 1.
- Figure 16 is a DSC chart of the crystalline form VIII of formula 1.
- Figure 17 is the XPRD pattern of the amorphous form IX of the adipate salt of formula 1.
- Figure 18 is the MDSC chart of the amorphous form IX of the adipate salt of formula 1.
- Figure 19 is the XPRD pattern of the fumarate amorphous form X of formula 1.
- Figure 20 is the MDSC chart of the fumarate amorphous form X of formula I.
- Figure 21 is the XPRD pattern of the amorphous form XI of the nicotinate of formula 1.
- Figure 22 is an MDSC chart of amorphous form XI of the nicotinate of formula 1.
- Figure 23 is the XPRD pattern of the amorphous form XII of the potassium salt of formula 1.
- Figure 24 is the MDSC chart of the amorphous form XII of the potassium salt of formula 1.
- Figure 25 is the XPRD pattern of the amorphous form XIII of the sodium salt of formula 1.
- Figure 26 is the MDSC chart of the amorphous form XIII of the sodium salt of formula 1.
- Figure 27 is the XPRD pattern of the amorphous form XIV of formula 1.
- Figure 28 is the MDSC chart of the amorphous form XIV of formula 1.
- Figure 29 is the TGA chart of the amorphous form XIV of formula 1.
- Figure 30 is the XPRD pattern of the amorphous form XV of the maleate salt of formula 1.
- Figure 31 is the MDSC chart of the amorphous form XV of the maleate salt of formula 1.
- Figure 32 is the TGA chart of the amorphous form XV of the maleate salt of formula 1.
- Figure 33 is the XPRD pattern of the amorphous form XVI of the meglumine salt of formula 1.
- Figure 34 is the MDSC chart of the amorphous form XVI of the meglumine salt of formula 1.
- Figure 35 is the TGA graph of the amorphous form XVI of the meglumine salt of formula 1.
- the slow volatilization test is carried out by selecting the compound of formula 1 and 8 different solvents or mixed solvents. In the experiment, weigh about 10 mg of the solid compound of formula 1 into a 1 mL glass bottle, add the corresponding solvent to dissolve to obtain a clear solution, then seal with Parafilm TM membrane and pierce 4 small holes with a needle, and place it at room temperature to slowly volatilize and crystallize. After the solid has precipitated, take out the solid to test XRPD (See Table 9) .
- the suspension stirring test selects the compound of formula 1 to be carried out under a variety of different conditions.
- a : _Preparation method Pipette 50 mL of 0.2 M potassium chloride solution into a 200-mL volumetric flask, add 134 mL of 0.2 M hydrochloric acid solution, and mix well. Add purified water close to the graduation line and adjust the pH to 1.0.
- Preparation method Pipette 25 mL of 0.2 M potassium dihydrogen phosphate (KH2PO4) solution and 11.2 mL of 0.2 M sodium hydroxide solution into a 100-mL volumetric flask. Add purified water close to the graduation line and adjust the pH to 6.8.
- KH2PO4 potassium dihydrogen phosphate
- the reverse anti-solvent addition experiment used the compound of formula 1 as the starting material and selected 19 different solvent/anti-solvent systems.
- the slow cooling test used the compound of formula 1 as the starting material, and a total of 9 different solvents or mixed solvents were selected.
- the gas-phase permeation test uses the compound of formula 1 as the starting material, and a total of 2 different solvent/antisolvent systems are selected.
- Table 13 show that only Amorphous was obtained in the test.
- the gas-solid permeation test used the compound of formula 1 as the starting material, and a total of 3 different solvents were selected.
- Table 14 show that only amorphous was obtained in the test.
- the crystalline form VIII of the compound of formula 1 Weigh 196 mg of the compound of formula 1, add 8 mL of tetrahydrofuran/n-hexane (1/19, v/v) mixed solvent, stir at 800 rpm at 50°C for 48 hours, then centrifuge to obtain the crystalline form VIII of the compound of formula 1
- Example 8 Optimization and preparation method of the crystalline form VI of the compound of formula 1
- the compound of formula 1 (15 mg) and fumaric acid (1.37 mg) at a molar ratio of 1: 1 were suspended and stirred in 0.5 mL of methanol at room temperature to obtain the crystalline form VI of the compound of formula 1.
- the method of adding anti-solvent is used instead of suspension stirring to realize the possibility of solid precipitation from a clear solution.
- acetone can dissolve the compound of formula 1 well without wall hanging, so the solvent containing acetone is selected as the positive solvent.
- the crystalline form VI can only be obtained in a solvent system containing methanol, and fumaric acid may not be added. Therefore, considering the simplicity of operation, the anti-solvent methanol was added dropwise to the acetone solution of the free base of the compound of formula 1 as an optimized method for preparing the crystalline form VI.
- Example 16 Hygroscopicity test of the Amorphous form XIV of the compound of formula 1 (DVS)
- Example 17 Stability test of the amorphous form XIV of the compound of formula 1
- the amorphous sample is packed with nitrogen, shading, and moisture-proof, and is stable under high humidity and light conditions; but it is sensitive to heat and needs to be stored at low temperature and protected from light.
- Example 18 Polymorphic screening test of the potassium salt of the compound of formula 1
- Example 20 Preparation of the amorphous form XV of the maleate salt of the compound of formula 1
- Test method The sample is placed in an aluminum pan, and the temperature is increased from room temperature to the set temperature at a rate of 10°C/min under N 2 protection after pressing the lid.
- MDSC Modulation Differential Scanning Calorimetry
- Measurement method The sample is placed in an aluminum pan, and after pressing the cover, it is heated from room temperature to the set temperature at a rate of 10°C/min under the protection of N2, and then kept at a constant temperature for 10 minutes, and then cooled to 35°C at a rate of 15°C/min. The temperature is raised to the specified temperature with a heating rate of 3 °C/min, a modulation period of 60 s, and a modulation amplitude of ⁇ 1 °C.
- thermogravimetric analyzer provided by TA Instruments
- Measurement method The sample is placed in an alloy pan, and the opening is heated from room temperature to the set temperature at a rate of 10°C/min under the protection of N 2 .
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Oncology (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Disclosed herein are a crystalline or amorphous form of a Bcl-2/Bcl-xL inhibitor compound or a salt thereof, and a preparation method and application thereof.
Description
The invention belongs to the field of medicinal chemistry, and particularly relates to a crystalline or amorphous form of a Bcl-2/Bcl-xL inhibitor compound or a salt thereof, and a preparation method and application thereof.
Apoptosis is the process of programmed cell death and an essential biological process for tissue homeostasis. Apoptosis resistance is a hallmark of human cancer. One of the main ways for cancer cells to evade apoptosis is through up-regulation of anti-apoptotic Bcl-2 family proteins. Targeting key apoptosis regulators to overcome apoptosis resistance and promote tumor cell apoptosis is a new cancer treatment strategy.
The Bcl-2 protein functions as a key regulator of apoptosis in both cancer cells and normal cells. The Bcl-2 protein serves as a check for apoptosis, allowing healthy and useful cells to survive. This protein family includes anti-apoptotic proteins, such as Bcl-2, Bcl-xL, and Mcl-1, and pro-apoptotic molecules, including Bid, Bim, Bad, Bak, and Bax. Although normal cells have low expression levels of anti-apoptotic Bcl-2 and Bcl-xL proteins, these proteins have been found to be highly overexpressed in many different types of human tumors. These overexpressions are related to the poor prognosis of several types of cancer, and are related to clinical resistance to chemotherapeutics and radiation. Consistent with clinical observations, laboratory studies have determined that overexpression of Bcl-2 or Bcl-xL causes cancer cells to become more resistant to chemotherapeutic agents in vitro and in vivo. Therefore, it has been sought to target Bcl-2 and/or Bcl-xL as a cancer treatment strategy. Inhibiting the Bcl-2 activity of cancer cells can reduce chemotherapy resistance and increase cancer cell killing.
Bcl-2 and Bcl-xL proteins inhibit apoptosis by heterodimerization with pro-apoptotic Bcl-2 family proteins such as Bak, Bax, Bim, Bid, Puma, and Bad. The experimentally determined three-dimensional structures of Bcl-xL and Bcl-2 have shown that these proteins have well-defined grooves that interact with the BH3 domain of the pro-apoptotic Bcl-2 protein. It has been proposed that a non-peptide small molecule designed to block the heterodimerization of the Bcl-2/Bcl-xL protein and its pro-death binding partner can be effective as an antagonist of Bcl-2/Bcl-xL, and the small Molecular inhibitors may have great therapeutic potential to treat human cancers in which Bcl-2 and/or Bcl-xL are highly expressed.
These discoveries make possible new strategies for drug discovery targeting cancer.
WO2014/113413A1 discloses a small molecule Bcl-2/Bcl-xL inhibitor for inducing apoptosis of cancer cells expressing Bcl-2 and/or Bcl-xL, and specifically discloses representative compounds: (R) -3- (1- (3- (4- (N- (4- (4- (3- (2- (4-chlorophenyl) -1-isopropyl-5-methyl-4- (methylsulfonyl) -1H-pyrrol-3-yl) -5-fluorophenyl) piperazin-1-yl) phenyl) sulfamoyl) -2- (trifluoromethylsulfonyl) phenylamino) -4- (phenylthio) buty l) piperidine-4-carbonyloxy) propylphosphonic acid (Hereinafter referred to as the compound of formula 1) , Its structural formula is as follows:
However, the current literature including the patent application mainly reports the structure and pharmacological activity of this type of compound, and has not conducted any research and report on its polymorphism, Amorphous and other structural polymorphisms.
Due to the influence of various factors such as molecular structure configuration, conformation, molecular arrangement, molecular force, eutectic substance, etc., solid materials cause the spatial arrangement of molecular lattices to be different, forming two or more different crystal structures. This phenomenon It is called "Polymorphism Phenomenon" or "Phenomenon" . "Polymorphism" is widespread in solid drugs, and there may be differences in physical and chemical properties between different crystal forms of the same drug, such as appearance, density, hardness, melting point, solubility, stability, dissolution rate, dissolution rate, bioavailability, etc. There may be significant differences, and this phenomenon is particularly obvious in oral solid preparations. In addition, the existence and quantity of polymorphic compounds are unpredictable. Different crystalline forms of the same drug may have significant differences in solubility, melting point, density, stability, etc., thereby affecting the uniformity and biological characteristics of the drug to varying degrees. Utilization, efficacy and safety, etc.
In addition to crystal forms, some solid compounds may also have Amorphous forms. Amorphous refers to the structure of some non-completely crystalline Amorphous regions (amorphous regions) or the formation of some Amorphous solids (amorphous) . For a specific solid drug, the existence and quantity of its Amorphous form are also unpredictable, and may also have a significant impact on the solubility, melting point, density, and stability of the drug. Therefore, in the process of new drug development, a comprehensive screening of crystalline and amorphous forms of drug compounds is required, considering multiple factors.
In particular, for the above-mentioned compound of formula 1 used as a Bcl-2/Bcl-xL inhibitor, the development of a crystalline form or an Amorphous form of the compound or its salt that can improve the stability, solubility, and bioavailability of the compound have potential medicinal and clinical value.
Summary of the invention
The invention provides a crystalline form or amorphous form of a Bcl-2/Bcl-xL inhibitor compound or a salt thereof, and a preparation method and application thereof. The crystalline form or Amorphous form of the present invention has very important value for drug development, formulation development and production.
In the following description, certain specific details are set forth in order to provide a thorough understanding of various embodiments of the present invention. However, those skilled in the art will understand that the present invention can be practiced without these details. The following description of several embodiments is made with the understanding that the present disclosure is regarded as an example of the claimed subject matter, and is not intended to limit the appended claims to the specific embodiments shown. The headings used throughout the present invention are provided for convenience only and should not be construed as limiting the claims in any way. The embodiment shown under any heading can be combined with the embodiment shown under any other heading.
In addition, when referring to, for example, XRPD diagrams, TGA diagrams, MDSC, DSC diagrams, etc., the term "substantially as shown" means that it is not necessarily the same as those described herein, but when considered by a person of ordinary skill in the art, The spectrum that falls within the limits of experimental error or deviation.
In one aspect, the present invention provides a crystalline or Amorphous form of the compound of formula 1 or its salts:
The chemical name of the compound is (R) -3- (1- (3- (4- (N- (4- (4- (3- (2- (4-chlorophenyl) -1-isopropyl-5-methyl-4- (methylsulfonyl) -1H-pyrrol-3-yl) -5-fluorophenyl) piperazin-1-yl) phenyl) sulfamoyl) -2- (trifluoromethylsulfonyl) phenylamino) -4- (phenylthio) buty l) piperidine-4-carbonyloxy) propylphosphonic acid. Specifically, the form may be the following specific forms:
1) The compound of formula 1 fumarate crystalline form I
In one embodiment, the form is the crystalline form I of the fumarate salt of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in an X-ray powder diffraction (XRPD) pattern expressed by an angle of 2θ: 18.03±0.2°, 28.81±0.2° and 31.88±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 1 below and/or an X-ray powder diffraction (XRPD) pattern basically as shown in FIG. 1.
Table 1
In some preferred embodiments, the form also has the following characteristics:
1) In the Modulated Differential Scanning Calorimetry (MDSC) , there is an endothermic peak at 217.4±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 2.
2) The compound of formula 1 hydrochloride crystalline form II
In one embodiment, the form is the crystalline form II of the hydrochloride salt of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ : 13.01±0.2°, 21.55±0.2°, 25.95±0.2°, 31.33±0.2° and 37.34±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 2 below and/or an XRPD pattern basically as shown in FIG. 3.
Table 2
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is an endothermic peak at 148.2±2.0℃ and a glass transition temperature at 139.5±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 4.
3) The compound of formula 1 nicotinate crystalline form III
In one embodiment, the form is the nicotinic acid salt crystalline form III of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram expressed at 2θ angles: 15.46±0.2° and 18.06±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 3 below and/or an XRPD pattern basically as shown in FIG. 5.
Table 3
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 127.6±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 6.
4) The compound of formula 1 sodium salt crystalline form IV
In one embodiment, the form is the sodium salt crystalline form IV of the compound of formula 1, which is characterized in that it has at least three or four characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 16.75±0.2°, 32.34±0.2°, 33.48±0.2° and 37.95±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 4 below and/or an XRPD pattern basically as shown in FIG. 7.
Table 4
5) The compound of formula 1 potassium salt crystalline form V
In one embodiment, the form is the crystalline form V of the potassium salt of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 24.35±0.2°, 30.10±0.2° and 31.41±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 5 below and/or an XRPD pattern basically as shown in FIG. 8.
Table 5
6) The Compound of formula 1 crystalline form VI
In one embodiment, the form is the crystalline form VI of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 3.94± 0.2°, 5.09±0.2°, 18.01±0.2°, 19.08±0.2°, 19.70±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 6 below and/or an XRPD pattern basically as shown in FIG. 9.
Table 6
In some preferred embodiments, the form also has the following characteristics:
1) In the Thermogravimetric Analysis (TGA) , there is a weight loss of 7.01±0.2%by weight before 160℃;
2) In the DSC chart, there are two endothermic peaks at 78.8±2.0℃ and 162.5±2.0℃;
3) Basically the TGA diagram shown in FIG. 10; and/or
4) Basically the DSC chart shown in FIG. 11.
7) The compound of formula 1 crystalline form VII
In one embodiment, the form is the crystalline form VII of the compound of formula 1, which is characterized in that it has at least three, at least four, or five characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 20.18± 0.2°, 20.74±0.2°, 29.20±0.2°, 29.28±0.2° and 36.73±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 7 below and/or an XRPD pattern basically as shown in FIG. 12.
Table 7
In some preferred embodiments, the form also has the following characteristics:
1) In the DSC chart, there are two endothermic peaks at 77.5±2.0℃ and 195.7±2.0℃; and/or
2) Basically the DSC chart shown in FIG. 13.
8) Compound of formula 1 crystalline form VIII
In one embodiment, the form is the crystalline form VIII of the compound of formula 1, which is characterized in that it has a characteristic peak at the following position in the XRPD diagram represented by an angle of 2θ: 18.11±0.2°.
In some preferred embodiments, the form has XRPD characteristic peaks at positions basically as shown in Table 8 below and/or an XRPD pattern basically as shown in FIG. 14.
Table 8
In some preferred embodiments, the form also has the following characteristics:
1) In the TGA chart, there is a weight loss of 7.59±0.2%by weight before 150℃;
2) In the DSC chart, there are two endothermic peaks at 69.3±2.0℃ and 152.1±2.0℃;
3) Basically the TGA diagram shown in FIG. 15; and/or
4) Basically, the DSC chart shown in FIG. 16.
9) Compound of Formula 1 Adipate Amorphous Form IX
In one embodiment, the form is the Amorphous Form IX of the adipate salt of the compound of Formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 17.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 113.4±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 18.
10) Compound of Formula 1 Fumarate Amorphous Form X
In one embodiment, the form is the fumarate Amorphous form X of the compound of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 19.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 136.6±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 20.
11) Compound of Formula 1 Nicotinate Amorphous Form XI
In one embodiment, the form is the Amorphous form XI of the compound nicotinate of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 21.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 117.9±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 22.
12) Compound of formula 1 potassium salt Amorphous form XII
In one embodiment, the form is the amorphous form XII of the potassium salt of the compound of formula 1, which is characterized in that it has an XRPD pattern basically as shown in FIG. 23.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 135.7±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 24.
13) Compound of formula 1 sodium salt morphous form XIII
In one embodiment, the form is the amorphous Form XIII of the sodium salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 25.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 140.9±2.0℃; and/or
2) Basically the MDSC chart shown in FIG. 26.
14) Amorphous form of compound of formula 1 XIV
In one embodiment, the form is the amorphous form XIV of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 27.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 148.9±2.0℃;
2) In the TGA chart, there is a weight loss of 6.35±0.2%by weight before 200℃;
3) Basically the MDSC chart shown in FIG. 28; and/or
4) Basically the TGA diagram shown in FIG. 29.
15) Compound of formula 1 maleate salt morphous form XV
In one embodiment, the form is the amorphous form XV of the maleate salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 30.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 91.89±2.0℃;
2) In the TGA chart, there is a weight loss of 8.767±0.2%by weight before 223.63℃;
3) Basically the MDSC chart shown in FIG. 31; and/or
4) Basically the TGA diagram shown in FIG. 32.
16)
Compound of formula 1 meglumine salt morphous form XVI
In one embodiment, the form is the amorphous form XVI of the meglumine salt of the compound of formula 1, which is characterized in that it has an XRPD pattern substantially as shown in FIG. 33.
In some preferred embodiments, the form also has the following characteristics:
1) In the MDSC chart, there is a glass transition temperature at 66.19±2.0℃;
2) In the TGA chart, there is a weight loss of 2.416±0.2%by weight before 124.19℃; a weight loss of 2.774±0.2%by weight between 187.71℃ and 124.19℃;
3) Basically the MDSC chart shown in FIG. 34; and/or
4) Basically the TGA diagram shown in FIG. 35.
In the second aspect, the present invention provides a method for preparing the crystalline or amorphous form of the compound of formula 1 or its salt.
In one embodiment, the present invention provides a method for preparing the crystalline form of the salt of the compound of formula 1, which comprises the following steps: mixing the compound of formula 1 with a solvent and an acid or base, and slowly volatilizing the resulting solution at room temperature to obtain the formula 1 The crystalline form of the salt of the compound.
The solvent can be a solvent commonly used in laboratories, such as one or more of water, alkane, alcoho, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbon solvents and polar aprotic solvents, such as DMF and DMSO. The mass-volume ratio of the compound of formula 1 to the solvent can be 100 mg: (0.1-10 mL) .
The acid may be a pharmaceutically acceptable acid or an acid commonly used in the art, and may be an inorganic acid or an organic acid. Further preferably, the acid may be fumaric acid, hydrochloric acid, niacin, maleic acid and the like. The base may be a pharmaceutically acceptable base or a base commonly used in the art. Further preferably, the alkali may be sodium hydroxide, potassium hydroxide, meglumine and the like.
In one embodiment, the present invention provides the preparation of the crystalline form VI of the compound of formula 1, which includes the following steps: dissolving the compound of formula 1 in acetone, then adding methanol and stirring, and separating the resulting solid to obtain crystalline form VI of the compound of formula 1.
In one embodiment, the present invention provides the preparation of crystalline form VII of the compound of formula 1, which comprises the following steps: dissolving the compound of formula 1 in ethyl acetate, then adding L-malic acid and stirring, and separating the resulting solid to obtain crystalline form VII of the compound of formula 1.
In one embodiment, the present invention provides the preparation of the crystalline form VIII of the compound of formula 1, which includes the following steps: adding the compound of formula 1 to a tetrahydrofuran/n-hexane mixed solvent, and after stirring, the resulting solid is separated to obtain crystalline form VIII of the compound of formula 1.
In one embodiment, the present invention provides a method for preparing the Amorphous form of the salt of the compound of formula 1, which comprises the following steps: mixing the compound of formula 1 with a solvent and an acid or base, and separating the resulting solid to obtain Amorphous form of the salt of the compound of formula 1
The solvent can be a solvent commonly used in laboratories, such as: One or more of water, alkane, alcohol, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbon solvents and polar aprotic solvents, such as DMF and DMSO. The mass-volume ratio of the compound of formula 1 to the solvent can be 100 mg: (0.1-10 mL) .
The acid may be a pharmaceutically acceptable acid or an acid commonly used in the art, and may be an inorganic acid or an organic acid. Further preferably, the acid may be fumaric acid, hydrochloric acid, niacin, maleic acid and the like. The base may be a pharmaceutically acceptable base or a base commonly used in the art. Further preferably, the alkali may be sodium hydroxide, potassium hydroxide, meglumine and the like.
In one embodiment, the present invention provides a method for preparing the Amorphous form XIV of the compound of formula 1, which comprises the following steps: adding the compound of formula 1 to a solvent to obtain a solution, then adding the resulting solution to an anti-solvent, and separating the obtained solid to obtain the Amorphous form XIV of the compound of formula 1.
Optionally, the solvent is selected from one or more of ethyl acetate, acetone, tetrahydrofuran, acetonitrile, DMSO, 1, 4-dioxane, MIBK, dichloromethane, and DMF; and/or the The anti-solvent is selected from one or more of heptane, ethanol, water, IPAc, IPA, butanol, and NPA.
In each of the above preparation methods, the preparation temperature can be a temperature that can be conventional in the art, for example, 20-50℃.
In the above preparation methods, the crystallization time is not particularly limited, as long as the crystals can be precipitated, for example, the crystallization time can be 1-48h.
In addition, the preparation method of the crystalline form or the amorphous form of the compound of formula 1 or its salt may also be a method well known in the art, such as solvent volatilization method, suspension stirring method, heating and cooling crystallization method, and mixed solvent crystallization method. The solvent volatilization method of the present invention is to volatilize the clear solution of the sample at different temperatures until the solvent is volatilized to dryness. The suspension stirring method of the present invention is to stir the supersaturated solution of the sample (with undissolved solids) in different solvents for a period of time. The heating and cooling crystallization method of the present invention is to dissolve the sample in an appropriate solvent under high temperature conditions, and after filtering, the filtrate is stirred and precipitated in a room temperature or low temperature environment. The mixed solvent crystallization method of the present invention is to take a sample and dissolve it in an appropriate solvent, add another or more solvents, and precipitate a solid system for a short time after stirring and filtering.
In the third aspect, the present invention provides a pharmaceutical composition comprising the crystalline or amorphous form of the compound of formula 1 or its salt, and pharmaceutically acceptable excipients.
The compound of formula 1 or its salt, crystalline form or amorphous form of a solvate may be a therapeutically effective amount. The pharmaceutically acceptable excipients may be well-known excipients in the art. In the case of solid preparations, they include, but are not limited to: diluents, binders, disintegrants, lubricants, glidants, release rate control agents, plasticizers, preservatives, antioxidants, etc.
The pharmaceutical composition can be selected in a dosage form suitable for human consumption, such as: tablets, capsules, granules, powdersor pills, etc., preferably tablets, capsules, granules, disintegrating tablets, sustained-release or controlled-release tablets etc.
The pharmaceutical composition of the present invention can be prepared by various methods well known in the art, and a therapeutically effective amount of one or more of the crystalline form or the amorphous form of the compound of formula 1 or its salt can be combined with one or more a variety of pharmaceutically acceptable excipients are mixed to prepare a dosage form suitable for human consumption, such as tablets, capsules, and granules.
"Therapeutically effective amount" refers to the amount of the compound form according to the present invention, which when administered to a patient in need thereof, is sufficient to achieve the treatment of a disease state, condition or disorder for which the compound is effective. Such an amount will be sufficient to elicit the biological or medical response of the tissue system or patient sought by the researcher or clinician.
In the fourth aspect, the present invention provides a crystalline form or Amorphous form of the compound of the above formula 1 or its salt or the above pharmaceutical composition for use in the treatment or prevention of diseases in which the inhibition of Bcl-2 or Bcl-xL provides benefits in medicine.
In one embodiment, the disease in which the inhibition of Bcl-2 or Bcl-xL provides benefit is cancer.
The crystalline form or amorphous form of the compound of formula 1 or its salt of the present invention has the following advantages:
1. In the present invention, a variety of unreported crystalline forms or amorphous forms of the compound of formula 1 or its salts have been discovered for the first time, and the forms can be used as an important basis for subsequent drug development, formulation development and production.
2. Various forms of the present invention including the amorphous form XIV of the compound of formula 1, the meglumine salt amorphous form XVI and the crystalline form VI of the compound of formula 1 have good physical and chemical stability.
3. The present invention improves the preparation method of the crystalline form VI of the compound of formula 1, thereby improving the purity of the product.
Description of the drawings
Figure 1 is the XPRD pattern of the crystalline form I of the fumarate salt of formula 1.
Figure 2 is the MDSC chart of the crystalline form I of the fumarate salt of formula 1.
Figure 3 is the XPRD pattern of the crystalline form II of the hydrochloride salt of formula 1.
Figure 4 is the MDSC chart of the crystalline form II of the hydrochloride salt of formula 1.
Figure 5 is the XPRD pattern of the crystalline form III of the Nicotinate of formula 1.
Figure 6 is the MDSC chart of the crystalline form III of the Nicotinateof formula 1.
Figure 7 is the XPRD pattern of the crystalline form IV of the sodium salt of formula 1.
Figure 8 is the XPRD pattern of the crystalline form V of the potassium salt of formula1.
Figure 9 is the XPRD pattern of the crystalline form VI of formula 1.
Figure 10 is the TGA diagram of the crystalline form VI of formula 1.
Figure 11 is a DSC chart of the crystalline form VI of formula 1.
Figure 12 is the XPRD pattern of the crystalline form VII of formula 1.
Figure 13 is a DSC chart of the crystalline form VII of formula 1.
Figure 14 is the XPRD pattern of the crystalline form VIII of formula 1.
Figure 15 is the TGA diagram of the crystalline form VIII of formula 1.
Figure 16 is a DSC chart of the crystalline form VIII of formula 1.
Figure 17 is the XPRD pattern of the amorphous form IX of the adipate salt of formula 1.
Figure 18 is the MDSC chart of the amorphous form IX of the adipate salt of formula 1.
Figure 19 is the XPRD pattern of the fumarate amorphous form X of formula 1.
Figure 20 is the MDSC chart of the fumarate amorphous form X of formula I.
Figure 21 is the XPRD pattern of the amorphous form XI of the nicotinate of formula 1.
Figure 22 is an MDSC chart of amorphous form XI of the nicotinate of formula 1.
Figure 23 is the XPRD pattern of the amorphous form XII of the potassium salt of formula 1.
Figure 24 is the MDSC chart of the amorphous form XII of the potassium salt of formula 1.
Figure 25 is the XPRD pattern of the amorphous form XIII of the sodium salt of formula 1.
Figure 26 is the MDSC chart of the amorphous form XIII of the sodium salt of formula 1.
Figure 27 is the XPRD pattern of the amorphous form XIV of formula 1.
Figure 28 is the MDSC chart of the amorphous form XIV of formula 1.
Figure 29 is the TGA chart of the amorphous form XIV of formula 1.
Figure 30 is the XPRD pattern of the amorphous form XV of the maleate salt of formula 1.
Figure 31 is the MDSC chart of the amorphous form XV of the maleate salt of formula 1.
Figure 32 is the TGA chart of the amorphous form XV of the maleate salt of formula 1.
Figure 33 is the XPRD pattern of the amorphous form XVI of the meglumine salt of formula 1.
Figure 34 is the MDSC chart of the amorphous form XVI of the meglumine salt of formula 1.
Figure 35 is the TGA graph of the amorphous form XVI of the meglumine salt of formula 1.
Specific implementation
Example
In the following examples, the experimental methods are completed according to conventional conditions or conventional test conditions, and the compounds used in the examples are obtained by commercially available or self-made methods.
Example 1: Preparation of crystalline form I of the fumarate salt of the compound of formula 1
Weigh 208mg of the compound of formula 1, add 4.2 mL of acetonitrile to prepare an acetonitrile solution, weigh 1.2 mg of fumaric acid into a glass vial, add 0.2 mL of the above-prepared acetonitrile solution, and after stirring the solution for 24 hours, it is observed that the sample is turbid. Continue to stir overnight, centrifuge to obtain a supernatant solution, and slowly evaporate the supernatant at room temperature to obtain the fumarate crystalline form I of the compound of formula 1.
Example 2: Preparation of crystalline form II of the hydrochloride salt of the compound of formula 1
Weigh 212 mg of the compound of formula 1, add 5.2 mL of methanol to prepare a methanol solution, add 6.5 μL of concentrated hydrochloric acid aqueous solution to a glass vial, and add 0.2 mL of the above-prepared methanol solution to obtain a clear solution. The above clear solution was slowly volatilized at room temperature to obtain the crystalline form II of the hydrochloride salt of the compound of formula 1.
Example 3: Preparation of crystalline form III of nicotinate of formula 1
Weigh 213mg of the compound of formula 1, add 4.2 mL of acetone/water (19/1, v/v) to prepare an acetone/water (19/1, v/v) solution, weigh 0.9 mg of niacin and add it to a glass vial, add 0.2 mL of the acetone/water (19/1, v/v) solution prepared above. After stirring the solution for 24 hours, it is observed that the sample is clear. Stirring is continued overnight, and the above liquid is slowly volatilized at room temperature to obtain the crystalline form III of nicotinate of formula 1.
Example 4: Preparation of crystalline form IV of the sodium salt of the compound of formula 1
Weigh 212 mg of the compound of formula 1 and add 5.2 mL of methanol to prepare a methanol solution. Weigh 0.54 mg of sodium hydroxide into a glass vial, add 0.2 mL of the methanol solution prepared above, and the solution is clear after stirring for 24 hours. The clear solution is slowly volatilized at room temperature to obtain the crystalline form IV of the sodium salt of the compound of formula 1
Example 5: Preparation of the crystalline form V of the potassium salt of the compound of formula 1
Weigh 208 mg of the compound of formula 1, add 4.2 mL of acetonitrile to prepare the acetonitrile solution of the compound of formula 1, weigh 0.4 mg of potassium hydroxide into a glass vial, add 0.2 mL of the above-prepared acetonitrile solution, and observe after stirring the solution for 24 hours When the solid precipitated, the sample was centrifuged after continuing to stir overnight, and the supernatant was placed at room temperature to slowly volatilize to obtain the crystalline form V of potassium salt of the compound of formula 1.
Example 6: Preparation of crystalline form VII of the compound of formula 1
Weigh 110 mg of the compound of formula 1, add 5.25 mL of ethyl acetate to prepare the ethyl acetate solution of the compound of formula 1, weigh 1.6 mg of L-malic acid into a glass vial, and add 0.75 mL of the prepared ethyl acetate solution. After the solution was stirred for 3 days, turbidity of the sample was observed, and the above sample was centrifuged to obtain the crystalline form VII of the compound of formula 1.
Example 7: Preparation of crystalline forms VIII of the compound of formula 1
In order to prepare the above crystalline form of the compound of formula 1, the crystallization process was screened as follows:
(1) Slow volatilization test of compound of formula 1
The slow volatilization test is carried out by selecting the compound of formula 1 and 8 different solvents or mixed solvents. In the experiment, weigh about 10 mg of the solid compound of formula 1 into a 1 mL glass bottle, add the corresponding solvent to dissolve to obtain a clear solution, then seal with Parafilm
TM membrane and pierce 4 small holes with a needle, and place it at room temperature to slowly volatilize and crystallize. After the solid has precipitated, take out the solid to test XRPD (See Table 9) .
Table 9
* The molar ratio of compound to tartaric acid is 1: 1.
(2) Suspension stirring test of compound of formula 1
The suspension stirring test selects the compound of formula 1 to be carried out under a variety of different conditions.
In the experiment, approximately 15 mg of the solid compound of formula 1 was weighed and placed in a 1 mL glass vial containing 0.5 mL of solvent, and the suspension was stirred at room temperature and 50℃ for 3 days. If there is precipitation, the solid is obtained after centrifugal separation and tested for XRPD. If there is no precipitation, use a slow volatilization method to precipitate solids (see Table 10) .
Table 10
a: _Preparation method: Pipette 50 mL of 0.2 M potassium chloride solution into a 200-mL volumetric flask, add 134 mL of 0.2 M hydrochloric acid solution, and mix well. Add purified water close to the graduation line and adjust the pH to 1.0.
b: _Preparation method: Pipette 50 mL of 0.2 M potassium hydrogen phthalate solution into a 200-mL volumetric flask, add 0.1 mL of 0.2 M hydrochloric acid solution, and mix well. Add purified water close to the graduation line and adjust the pH to 4.0.
c: Preparation method: Pipette 25 mL of 0.2 M potassium dihydrogen phosphate (KH2PO4) solution
and 11.2 mL of 0.2 M sodium hydroxide solution into a 100-mL volumetric flask. Add purified water
close to the graduation line and adjust the pH to 6.8.
(3) The reverse anti-solvent addition test of the compound of formula 1
The reverse anti-solvent addition experiment used the compound of formula 1 as the starting material and selected 19 different solvent/anti-solvent systems.
In the experiment, weigh about 15 mg of the compound of formula 1 and add 0.1-1.0 mL of the corresponding solvent to obtain a clear solution. The above solutions were added to 2.0 mL of anti-solvent under magnetic stirring conditions, and stirred at room temperature for 70 hours. If there is precipitation, the solid is obtained after centrifugal separation and tested for XRPD. If there is no precipitation, use a slow volatilization method to precipitate solids (see Table 11) .
Table 11
(4) Slow cooling test of compound of formula 1
The slow cooling test used the compound of formula 1 as the starting material, and a total of 9 different solvents or mixed solvents were selected. In the experiment, weigh about 15 mg of the compound of formula 1 into a 1 mL glass vial, and then add 0.6 mL of the corresponding solvent. All the obtained suspensions were placed in a 50℃ thermostat to equilibrate for 30 minutes. If the sample was dissolved, the solids were added continuously until a suspension at that temperature was obtained. Filter all the suspension with a syringe (filter membrane pore size is 0.45μm) , place the filtered clear filtrate in a 50℃ thermostat, and cool to 5℃ at a rate of 0.1℃/min. If there is precipitation, centrifuge to obtain a solid and test for XRPD. If there is no precipitation, use a slow volatilization method to precipitate solids (see Table 12) .
Table 12
(5) Gas phase permeation test of compound of formula 1
The gas-phase permeation test uses the compound of formula 1 as the starting material, and a total of 2 different solvent/antisolvent systems are selected. In the experiment, weigh about 10 mg of the compound of formula 1 into a 3 mL glass vial, and add a solvent to dissolve it until it is clear. Take 3 mL of anti-solvent into a 20-mL glass bottle, put the 3 mL glass vial containing the sample solution into the 20 mL glass bottle, and close the bottle cap tightly. Observe the phenomenon after standing for one week. If there is a solid precipitation, the solid is obtained after centrifugal separation and XRPD test is performed. If there is no precipitation, use a slow volatilization method to precipitate solids. The results in Table 13 show that only Amorphous was obtained in the test.
Table 13
Experiment number | Solvent | Anti-solvent | Form | |
1 | 1, 4-Dioxane | | Amorphous XIV | |
2 | DCM | IPAc | Amorphous XIV |
(6) Gas-solid permeability test of compound of formula 1
The gas-solid permeation test used the compound of formula 1 as the starting material, and a total of 3 different solvents were selected. In the experiment, weigh about 10 mg of the compound of formula 1 into a 3 mL glass vial, take 2 mL of the corresponding solvent into a 20 mL glass bottle, then put the 3 mL glass vial containing the sample into the 20 mL glass bottle, and close the bottle cap tightly. After standing for one week, observe the phenomenon, if there is solid, take it out for XRPD test. If the solid has been completely dissolved, the solid will be precipitated out using a slow volatilization method. The results in Table 14 show that only amorphous was obtained in the test.
Table 14
Experiment number | Solvent | Form | |
1 | | Amorphous XIV | |
2 | EtOAc | Amorphous XIV | |
3 | Acetonitrile | Amorphous XIV |
The preparation methods of the crystalline forms VIII of the compound of formula 1 obtained through the above-mentioned crystal form screening experiment are as follows:
The crystalline form VIII of the compound of formula 1: Weigh 196 mg of the compound of formula 1, add 8 mL of tetrahydrofuran/n-hexane (1/19, v/v) mixed solvent, stir at 800 rpm at 50℃ for 48 hours, then centrifuge to obtain the crystalline form VIII of the compound of formula 1
Example 8: Optimization and preparation method of the crystalline form VI of the compound of formula 1
The crystalline form VI was found during the salt formation screening with fumaric acid, so the preparation method of the crystalline form VI of the compound of formula 1 is as follows:
The compound of formula 1 (15 mg) and fumaric acid (1.37 mg) at a molar ratio of 1: 1 were suspended and stirred in 0.5 mL of methanol at room temperature to obtain the crystalline form VI of the compound of formula 1.
But the residual fumaric acid in the obtained crystalline form VI sample is difficult to remove, which affects the purity of the crystalline form VI. Therefore, this Example optimizes the preparation method of crystalline form VI. Table 15 is a summary of optimization experiments on the preparation method of crystalline form VI.
First of all, the method of adding anti-solvent is used instead of suspension stirring to realize the possibility of solid precipitation from a clear solution. Secondly, compared with methanol, acetone can dissolve the compound of formula 1 well without wall hanging, so the solvent containing acetone is selected as the positive solvent. In addition, it can be seen from the test results in Table 15 that the crystalline form VI can only be obtained in a solvent system containing methanol, and fumaric acid may not be added. Therefore, considering the simplicity of operation, the anti-solvent methanol was added dropwise to the acetone solution of the free base of the compound of formula 1 as an optimized method for preparing the crystalline form VI. Specifically, 100 mg of the compound of formula 1 was weighed and dissolved in 1.0 mL of acetone, and 5.0 mL of methanol was added dropwise under magnetic stirring (1000 rpm) , stirred at room temperature for 48 hours, and centrifuged to obtain the crystalline form VI of compound of formula 1.
Table 15
Example 9: Investigation on the physicochemical stability of the crystalline form VI of the compound of formula 1
After placing the crystalline form VI of formula 1 at 25 ℃/60%RH and 40 ℃/75%RH for one week, the purity and crystal form did not change significantly (See table 16) , indicating that the crystalline form VI The physical and chemical properties are stable when stored for one week under the conditions of 25 ℃/60%RH and 40 ℃/75%RH.
Table 16
Example 10: Preparation of Amorphous Form IX of Adipate Compound of Formula 1
Weigh 195 mg of the compound of formula 1 and 22.6 mg of adipic acid, and add 2 mL of tetrahydrofuran/water (19/1, v/v) . The above sample was stirred at room temperature for 3 days, and then centrifuged to separate solid and liquid. The supernatant was taken out and added with 2 mL of n-heptane for addition of the anti-solvent, and precipitated to obtain the Amorphous Form IX of the adipate compound of Formula 1
Example 11: Preparation of Amorphous Form X of Fumarate Compound of Formula 1
Weigh 21.0 mg of the compound of formula 1 and 1.6 mg of fumaric acid, add 0.2 mL of acetonitrile, stir the above sample at 5 ℃ for 2 days, then place it at -20 ℃ for 2 days, and precipitate to obtain the Amorphous Form X of Fumarate Compound of Formula 1
Example 12: Preparation of Amorphous Form XI of Nicotinate Compound of Formula 1
Weigh 209 mg of the compound of formula 1, add 4.2 mL of tetrahydrofuran/water (19/1, v/v) to prepare a tetrahydrofuran/water (19/1, v/v) solution, weigh 0.84 mg of niacin and add it to a glass vial, add 0.2 mL of the above-prepared tetrahydrofuran/water (19/1, v/v) solution. After stirring the solution for 24 hours, the sample is observed to be clear. Continue stirring overnight, and slowly evaporate the above liquid at room temperature to obtain the Amorphous Form XI of Nicotinate Compound of Formula 1.
Example 13: Preparation of Amorphous Form XII of the potassium salt of the compound of formula 1
Weigh 19.6 mg of the compound of formula 1, add 1.1 mg of potassium hydroxide and 0.2 mL of acetonitrile, stir the above sample at 5 ℃ for 2 days, then place it at -20 ℃ for 2 days, and precipitate to obtain the Amorphous form XII of the potassium salt of the compound of formula 1.
Example 14: Preparation of Amorphous Form XIII of Sodium Salt of the compound of Formula 1
Weigh 19.8 mg of the compound of formula 1, add 0.8 mg sodium hydroxide and 0.2 mL acetonitrile. After stirring the above sample at 5 ℃ for 2 days, it was placed at -20 ℃ and allowed to stand for 2 days. The solid precipitated out to obtain the sodium salt Amorphous form XIII of the compound of formula 1.
Example 15: Preparation of Amorphous Form XIV of Compound of Formula 1
Weigh about 15 mg of the compound of formula 1, and add 0.1-1.0 mL of EtOAc to obtain a clear solution. The above solution was added to 2.0 mL of heptane under magnetic stirring, and stirred at room temperature for 70 hours. The precipitate is separated by centrifugation, and the Amorphous form XIV of the compound of formula 1 is obtained.
Example 16: Hygroscopicity test of the Amorphous form XIV of the compound of formula 1 (DVS)
Take about 10 mg of the Amorphous form XIV sample of the compound of formula I for dynamic water adsorption (DVS) test. The conclusions are described in Table 17 below:
Table 17
Name | Moisture gain (80%RH) |
Amorphous XIV | 4.83% (Hygroscopic) |
The above results indicate that Amorphous samples easily absorb water during storage and need to be kept tightly closed.
Example 17: Stability test of the amorphous form XIV of the compound of formula 1
Pack the amorphous sample with nitrogen, shading, and moisture-proof, and place it under 40℃, high humidity 92.5%RH, light conditions, and take samples at 0 days/5 days/10 days to investigate its content, related substances and crystal forms (light conditions: total illuminance≥1.2×106 Lux·hr, near ultraviolet energy≥200w·hr/m2) . The results are shown in Table 18.
Table 18
Condition | Content (%) | Total impurities (%) |
0 Days | 99.5 | 0.39 |
5 Days-high humidity | 100.7 | 0.37 |
5 Days-light | 100.4 | 0.43 |
5 Days-40℃ | 100.3 | 0.59 |
10 Days-high humidity | 99.7 | 0.39 |
10 Days-light | 100.1 | 0.41 |
10 Days -40℃ | 99.7 | 0.64 |
The results show that the amorphous sample is packed with nitrogen, shading, and moisture-proof, and is stable under high humidity and light conditions; but it is sensitive to heat and needs to be stored at low temperature and protected from light.
Example 18: Polymorphic screening test of the potassium salt of the compound of formula 1
Weigh about 50 mg of the potassium salt amorphous form XII into a 4 mL glass bottle, and gradually add 0.4 mL of a single or mixed solvent (see Table 19) to prepare a suspension sample in the glass bottle, and add the magnets at 40℃. After stirring for 3 days, it was centrifuged, and the remaining solid was dried in a vacuum drying oven at 25℃.
Table 19
Experiment Number | Solvent | Form | |
- | Initial form | Amorphous XII | |
1 | Methanol | Basically |
|
2 | Ethanol | Amorphous XII | |
3 | Acetonitrile | Amorphous XII | |
4 | | Amorphous XII | |
5 | Ethyl acetate | Amorphous XII | |
6 | Tetrahydrofuran | Oily | |
7 | n-Heptane | Amorphous XII | |
8 | 1, 4-Dioxane | Amorphous XII | |
9 | | Amorphous XII | |
10 | Methanol: Water 3: 1 (v: v) | Amorphous XII | |
11 | Ethanol: Water 3: 1 (v: v) | Amorphous XII | |
12 | Acetonitrile: Water 1: 1 (v: v) | Amorphous XII | |
13 | Acetone: Water 1: 2 (v: v) | Amorphous XII |
Example 19: Polymorph screening test of the sodium salt of the compound of formula 1
Weigh about 50 mg of the sodium salt amorphous form XIII into a 4 mL glass bottle, and gradually add 0.4 mL of a single or mixed solvent (see Table 20) to prepare a suspension sample in the glass bottle. After adding the magnet, it is heated at 40℃. After stirring for 3 days, it was centrifuged, and the remaining solid was dried in a vacuum drying oven at 25℃.
Table 20
Example 20: Preparation of the amorphous form XV of the maleate salt of the compound of formula 1
Weigh 19.2 g of the compound of formula 1 and place it in a 40mL glass bottle, add 30 mL of acetone for ultrasonic solubilization, add a magnet and stir on a stirrer, then add 19.2 g of maleic acid. After stirring overnight at room temperature, it becomes slightly turbid. After adding 20 mL of n-hexane, a precipitate was precipitated. After stirring overnight, the amorphous form XV of the maleate salt of the compound of formula 1 was obtained after centrifugal separation.
Example 21: Polymorphic Screening Test of Maleate Compound of Formula 1
Weigh about 50 mg of the maleate amorphous form XV into a 4 mL glass bottle, and gradually add 0.4 mL of a single or mixed solvent (see Table 21) to prepare a suspension sample in the glass bottle. After adding the magnet after stirring for 3 days at 40℃, it was centrifuged, and the remaining solid was placed in a vacuum drying oven at 25℃ to dry.
Table 21
Example 22: Preparation of the amorphous form XVI of the meglumine salt of the compound of formula 1
Weigh 19.2 g of the compound of formula 1 and place it in a 40mL glass bottle, add 30 mL of acetone for ultrasonic solubilization, add a magnet and stir on a stirrer, and then add 19.2 g of meglumine. After stirring overnight at room temperature, it becomes slightly turbid. After adding 30 mL of n-hexane, a precipitate was precipitated. After stirring overnight, the amorphous form XVI of the meglumine salt of the compound of formula 1 was obtained after centrifugal separation.
Example 23: Polymorph screening test of meglumine salt of the compound of formula 1
(1) Weigh about 50 mg of the amorphous form of meglumine salt XVI in a 4 mL glass bottle, and gradually add 0.4 mL of single or mixed solvent (see Table 22) to prepare a suspension sample in the glass bottle, and add magnet After the seeds were stirred at 40℃ for 3 days and then centrifuged, the remaining solid was placed in a vacuum drying oven at 25℃ to dry.
Table 22
(2) Weigh about 50 mg of meglumine salt amorphous form XVI in a 1.5 mL glass bottle, and gradually add 0.3 mL of single or mixed solvent (see Table 23) to prepare a suspension sample in the glass bottle, and add magnet After the seeds were stirred at room temperature for 3 days and then centrifuged, the remaining solid was placed in a vacuum drying oven at 25℃ to dry.
Table 23
(3) Weigh about 30 mg of meglumine salt amorphous form XVI in a 1.5 mL glass bottle, gradually add the corresponding solvent (see Table 24) to dissolve it, and then filter it with a 0.22 μm organic filter membrane into a rinsed glass bottle, seal it with aluminum foil paper, pierce the hole, and place it at room temperature to evaporate slowly.
Table 24
(4) Weigh about 30 mg of meglumine salt amorphous form XVI in an 8 mL glass bottle, first add a good solvent (see Table 25) to dissolve it, and then gradually add 5 mL of anti-solvent n-heptane to obtain a suspension After centrifugation, the residual solid of the sample was dried in a vacuum drying oven at 25℃.
Table 25
Number | Solvent | Form | |
1 | Dichloromethane /0.2 | Amorphous XVI | |
2 | Methanol /0.3 mL | Amorphous XVI | |
3 | Tetrahydrofuran /0.2 mL | Amorphous XVI | |
4 | 2-methyltetrahydrofuran /0.5 mL | Amorphous XVI |
5 | Dimethyl sulfoxide /0.2 mL | Amorphous XVI |
6 | N-methylpyrrolidone /0.2 mL | Amorphous XVI |
Example 24: Stability test of salt form screening
Take an appropriate amount of the compound into an 8mL glass bottle, and then place it at room temperature (25℃, open) , high humidity (room temperature/75%RH, open) and light (room temperature, white light: 6980 lux, UV 282 μW/cm2) . Take samples for testing on the 5th, 10th, and 30th day (HPLC, XRPD) .
Table 26
The results showed that the XRPD of the salt form was unchanged under the above conditions.
Example 25: Identification and Characterization of Compound Form I-XVI of Formula 1
The instruments used and their parameters are as follows:
1. XPRD-X-ray powder diffraction
2. Differential scanning calorimetry (DSC)
Instrument: TA Q200/Q2000 differential scanning calorimeter provided by TA Instruments
Test method: The sample is placed in an aluminum pan, and the temperature is increased from room temperature to the set temperature at a rate of 10℃/min under N
2 protection after pressing the lid.
3. Modulation Differential Scanning Calorimetry (MDSC)
Instrument: TA Q200/Q2000 differential scanning calorimeter provided by TA Instruments
Measurement method: The sample is placed in an aluminum pan, and after pressing the cover, it is heated from room temperature to the set temperature at a rate of 10℃/min under the protection of N2, and then kept at a constant temperature for 10 minutes, and then cooled to 35℃ at a rate of 15℃/min. The temperature is raised to the specified temperature with a heating rate of 3 ℃/min, a modulation period of 60 s, and a modulation amplitude of ±1 ℃.
4. Thermogravimetric Analysis (TGA)
Instrument: TA Q500/Q5000 thermogravimetric analyzer provided by TA Instruments
Measurement method: The sample is placed in an alloy pan, and the opening is heated from room temperature to the set temperature at a rate of 10℃/min under the protection of N
2.
For the identification and characterization results of XPRD, TGA and (M) DSC, please refer to Figure 1-35, Table 1-8 and related text description.
Each reference, including all patents, patent applications, and publications cited in this application, is incorporated herein by reference in its entirety, as if each of them were individually incorporated. In addition, it will be understood that in the above teachings of the present invention, those skilled in the art can make certain changes or modifications to the present invention, and these equivalents will still fall within the scope of the present invention defined by the appended claims of the application.
Claims (36)
- The form of claim 1, which is the crystalline form I of the fumarate salt of the compound of formula 1, characterized in that it has characteristic peaks at the following positions in an X-ray powder diffraction (XRPD) pattern represented by an angle of 2θ: 18.03±0.2°, 28.81±0.2°and 31.88±0.2°.
- The form of claim 2, which has XRPD characteristic peaks at positions substantially as shown in Table 1 and/or an XRPD pattern substantially as shown in FIG. 1, and optionally has the following characteristics:1) In the Modulated Differential Scanning Calorimetry (MDSC) , there is an endothermic peak at 217.4±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 2.
- The form of claim 1, which is the crystalline form II of the hydrochloride salt of the compound of formula 1, characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 13.01±0.2°, 21.55±0.2°, 25.95±0.2°, 31.33±0.2°and 37.34±0.2°.
- The form of claim 4, which has XRPD characteristic peaks at positions substantially as shown in Table 2 and/or an XRPD pattern substantially as shown in FIG. 3, and optionally has the following characteristics:1) In the MDSC chart, there is an endothermic peak at 148.2±2.0℃and a glass transition temperature at 139.5±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 4.
- The form of claim 1, which is the nicotinate crystalline form III of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram expressed at 2θ angles: 15.46±0.2° and 18.06±0.2° .
- The form of claim 6, which has XRPD characteristic peaks at positions substantially as shown in Table 3 and/or an XRPD pattern substantially as shown in FIG. 5, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 127.6±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 6.
- The form of claim 1, which is the sodium salt crystalline form IV of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram expressed at 2θ angles: 16.75±0.2°, 32.34±0.2°, 33.48±0.2° and 37.95±0.2°.
- The form of claim 8, which has XRPD characteristic peaks at positions basically as shown in Table 4 and/or an XRPD pattern basically as shown in FIG. 7.
- The form of claim 1, which is the crystalline form V of the potassium salt of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 24.35±0.2°, 30.10±0.2° and 31.41±0.2°.
- The form of claim 10, which has XRPD characteristic peaks at positions basically as shown in Table5 and/or an XRPD pattern basically as shown in FIG. 8.
- The form of claim 1, which is the crystalline form VI of the compound of formula 1, which is characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by an angle of 2θ: 3.94±0.2°, 5.09±0.2°, 18.01±0.2°, 19.08±0.2°, 19.70±0.2°.
- The form of claim 12, which has XRPD characteristic peaks at positions basically as shown in Table6 and/or an XRPD pattern substantially as shown in FIG. 9, and optionally has the following characteristics:1) In the Thermogravimetric Analysis (TGA) , there is a weight loss of 7.01±0.2%by weight before 160℃;2) In the Differential Scanning Calorimetry (DSC) , there are two endothermic peaks at the peak temperature of 78.8±2.0℃ and -162.5±2.0℃;3) Basically the TGA diagram shown in FIG. 10; and/or4) Basically the DSC chart shown in FIG. 11.
- The form of claim 1, which is the crystalline form VII of the compound of formula 1, characterized in that it has characteristic peaks at the following positions in the XRPD diagram represented by 2θangles: 20.18±0.2°, 20.74±0.2°, 29.20± 0.2°, 29.28±0.2° and 36.73±0.2°.
- The form of claim 14, which has XRPD characteristic peaks at positions basically as shown in Table7 and/or an XRPD pattern substantially as shown in FIG. 12, and optionally has the following characteristics:1) In the DSC chart, there are two endothermic peaks at peak temperatures of 77.5±2.0℃ and 195.7±2.0℃; and/or2) Basically the DSC chart shown in FIG. 13.
- The form of claim 1, which is the crystalline form VIII of the compound of formula 1, which is characterized in that it has a characteristic peak at the following position in the XRPD diagram represented by an angle of 2θ: 18.11±0.2°.
- The form of claim 16, which has XRPD characteristic peaks at positions basically as shown in Table 8 and/or an XRPD pattern substantially as shown in FIG. 14, and optionally has the following characteristics:1) In the TGA chart, there is a weight loss of 7.59±0.2%by weight before 150℃;2) In the DSC chart, there are two endothermic peaks at peak temperatures of 69.3±2.0℃ and 152.1±2.0℃;3) Basically the TGA diagram shown in FIG. 15; and/or4) Basically the DSC chart shown in FIG. 16.
- The form of claim 1, which is the adipate salt Amorphous form IX of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 17, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 113.4±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 18.
- The form of claim 1, which is the fumarate Amorphous form X of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 19, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 136.6±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 20;
- The form of claim 1, which is the nicotinic acid salt Amorphous form XI of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 21, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 117.9±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 22.
- The form of claim 1, which is the Amorphous form XII of the potassium salt of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 23, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 135.7±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 24.
- The form of claim 1, which is the Amorphous form XIII of the sodium salt of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 25, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 140.9±2.0℃; and/or2) Basically the MDSC chart shown in FIG. 26.
- The form of claim 1, which is the Amorphous form XIV of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 27, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 148.9±2.0℃;2) In the TGA chart, there is a weight loss of 6.35±0.2%by weight before 200℃;3) Basically the MDSC chart shown in FIG. 28; and/or4) Basically the TGA diagram shown in FIG. 29.
- The form of claim 1, which is the amorphous form XV of the maleate salt of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 30, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 91.89±2.0℃;2) In the TGA chart, there is a weight loss of 8.767±0.2%by weight before 223.63℃;3) Basically the MDSC chart shown in FIG. 31; and/or4) Basically the TGA diagram shown in FIG. 32.
- The form of claim 1, which is the amorphous form XVI of the meglumine salt of the compound of formula 1, characterized in that it has an XRPD pattern substantially as shown in FIG. 33, and optionally has the following characteristics:1) In the MDSC chart, there is a glass transition temperature at 66.19±2.0;2) In the TGA chart, there is a weight loss of 2.416±0.2%by weight before 124.19℃; a weight loss of 2.774±0.2%by weight between 187.71℃ and 124.19℃;3) Basically the MDSC chart shown in FIG. 34; and/or4) Basically the TGA diagram shown in FIG. 35.
- The method for preparing the crystalline form of the salt of the compound of formula 1 according to any one of claims 1-11, which comprises the following steps: mixing the compound of formula 1 with a solvent and an acid or a base, and the resulting solution is slowly volatilized at room temperature, and This gives the crystalline form of the salt of the compound of formula 1;Optionally, wherein the solvent is selected from One or more of water, alkane, alcohol, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbons solvents and polar aprotic solvents such as DMF and DMSO;And/or the mass-volume ratio of the compound of formula 1 to the solvent is 100mg: (0.1-10mL) .
- The method of claim 26, wherein the acid is selected from fumaric acid, hydrochloric acid, niacin and maleic acid; and/or the base is selected from sodium hydroxide, potassium hydroxide, and meglumine.
- The method for preparing the crystalline form VII of the compound of formula 1 according to claim 14 or 15, which comprises the following steps: dissolving the compound of formula 1 in ethyl acetate, then adding L-malic acid and stirring, and separating the obtained solid to obtain the crystalline form VII of the compound of formula 1.
- The method for preparing the crystalline form VI of the compound of formula 1 according to claim 12 or 13, which comprises the following steps: dissolving the compound of formula 1 in acetone, then adding methanol and stirring, and separating the obtained solid to obtain the crystalline form VI of the compound of formula 1.
- The method for preparing the crystalline form VIII of the compound of formula 1 according to claim 16 or 17, which comprises the following steps: adding the compound of formula 1 to a tetrahydrofuran/n-hexane mixed solvent, and after stirring, the obtained solid is separated to obtain the crystalline form VIII of the compound of formula 1.
- The method for preparing the Amorphous form of the salt of the compound of formula 1 according to any one of claims 18-22、24-25, which comprises the steps of: mixing the compound of formula 1 with a solvent and an acid or base, and separating the resulting solid to obtain the compound of formula 1 Amorphous form of salt; Optionally, wherein the solvent is selected from one or more of water, alkane, alcohol, ketone, ester, aromatic hydrocarbon, halogenated hydrocarbon, nitrile, ether, aliphatic hydrocarbons solvents, polar aprotic solvents such as DMF and DMSO, and/or the mass-volume ratio of the compound of formula 1 to the solvent is 100mg: (0.1-10mL) .
- The method of claim 31, wherein the acid is selected from fumaric acid, hydrochloric acid, niacin, and maleic acid; and/or the base is selected from sodium hydroxide, potassium hydroxide, and meglumine.
- The method for preparing the Amorphous form XIV of the compound of formula 1 according to claim 23, which comprises the following steps: adding the compound of formula 1 to a solvent to obtain a solution, then adding the obtained solution to an anti-solvent, and separating the obtained solid to obtain the Amorphous form XIV of compound of formula 1; Optionally, the solvent is selected from one or more of ethyl acetate, acetone, tetrahydrofuran, acetonitrile, DMSO, 1, 4-dioxane, MIBK, dichloromethane, and DMF; and/or the anti-solvent is selected from one or more of heptane, ethanol, water, IPAc, IPA, butanol, and NPA.
- A pharmaceutical composition comprising the crystalline or amorphous form of the compound of Formula 1 or its salt according to any one of claims 1-25, and pharmaceutically acceptable excipients.
- The crystalline form or amorphous form of the compound of formula 1 or its salt according to any one of claims 1-25 or the pharmaceutical composition of claim 32 is prepared for the treatment or prevention of Bcl-2 or Bcl-xL in a medicine to suppress a disease that provides benefits; preferably, the disease is cancer.
- The use of claim 35, wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, colon cancer, kidney cancer, liver cancer, lung cancer, ovarian cancer, prostate cancer, testicular cancer, genitourinary tract cancer, lymphatic system cancer, rectal cancer , Laryngeal cancer, pancreatic cancer, esophageal cancer, gastric cancer, gallbladder cancer, cervical cancer, thyroid cancer, skin cancer; hematopoietic tumors of the lymphatic system, including leukemia, acute lymphoblastic leukemia, acute lymphoblastic leukemia, B-cell lymph Tumor, T-cell lymphoma, Hodgkin’s lymphoma, non-Hodgkin’s lymphoma, hair cell lymphoma, histiocytic lymphoma and Burketts lymphoma; hematopoietic tumors of the bone marrow line, including acute and chronic myelogenous leukemia, bone marrow Dysplasia syndrome, myelogenous leukemia and promyelocytic leukemia; tumors of the central and peripheral nervous system, including astrocytoma, neuroblastoma, glioma and schwannoma; tumors of mesenchymal origin, Including fibrosarcoma, rhabdomyosarcoma and osteosarcoma; and other tumors, including melanoma, xeroderma pigmentosum, keratoacanthoma, seminoma, thyroid follicular carcinoma, teratoma, renal cell carcinoma (RCC) , Myeloma, myelogenous and lymphoblastic leukemia, neuroblastoma and glioblastoma.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18/015,883 US20230271986A1 (en) | 2020-07-13 | 2021-07-12 | Crystalline or Amorphous form of Bcl-2/Bcl-xL inhibitor compound or its salts |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010671412 | 2020-07-13 | ||
CN202010671412.3 | 2020-07-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2022012481A1 true WO2022012481A1 (en) | 2022-01-20 |
Family
ID=79274458
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2021/105856 WO2022012481A1 (en) | 2020-07-13 | 2021-07-12 | Crystalline or Amorphous form of Bcl-2/Bcl-xL inhibitor compound or its salts |
Country Status (3)
Country | Link |
---|---|
US (1) | US20230271986A1 (en) |
CN (1) | CN113929715A (en) |
WO (1) | WO2022012481A1 (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105246882A (en) * | 2013-01-16 | 2016-01-13 | 密执安大学评议会 | BCL-2/Bcl-xL inhibitors and therapeutic methods using the same |
CN110960537A (en) * | 2018-07-31 | 2020-04-07 | 苏州亚盛药业有限公司 | Combination of Bcl-2/Bcl-xL inhibitor and chemotherapeutic drug and application thereof |
WO2020140956A1 (en) * | 2019-01-04 | 2020-07-09 | Ascentage Pharma (Suzhou) Co., Ltd. | Process for preparing sulfonamide compounds |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2981753A1 (en) * | 2015-02-06 | 2016-08-11 | Unity Biotechnology, Inc. | Compounds and uses in treatment of senescence-associated conditions |
-
2021
- 2021-07-12 CN CN202110786296.4A patent/CN113929715A/en active Pending
- 2021-07-12 WO PCT/CN2021/105856 patent/WO2022012481A1/en active Application Filing
- 2021-07-12 US US18/015,883 patent/US20230271986A1/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105246882A (en) * | 2013-01-16 | 2016-01-13 | 密执安大学评议会 | BCL-2/Bcl-xL inhibitors and therapeutic methods using the same |
CN110960537A (en) * | 2018-07-31 | 2020-04-07 | 苏州亚盛药业有限公司 | Combination of Bcl-2/Bcl-xL inhibitor and chemotherapeutic drug and application thereof |
WO2020140956A1 (en) * | 2019-01-04 | 2020-07-09 | Ascentage Pharma (Suzhou) Co., Ltd. | Process for preparing sulfonamide compounds |
Also Published As
Publication number | Publication date |
---|---|
CN113929715A (en) | 2022-01-14 |
US20230271986A1 (en) | 2023-08-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2018196687A1 (en) | New crystal form of lenvatinib methanesulfonate and preparation method thereof | |
WO2016124137A1 (en) | Phosphate of epidermal growth factor receptor inhibitor, crystalline form of phosphate, and preparation method | |
KR20190093651A (en) | Novel crystalline forms of ((5- (3-chlorophenyl) -3-hydroxypyridine-2-carbonyl) amino) acetic acid and preparation methods thereof | |
TW201402563A (en) | Form I crystal of dimaleate salt of tyrosine kinase inhibitor and preparation method thereof | |
CN104470920A (en) | Solid state form of vemurafenib choline salt | |
WO2023040513A1 (en) | Crystal form of amg510 compound, and preparation method therefor and use thereof | |
US9884856B2 (en) | Crystal form of Dabrafenib mesylate and preparation method thereof | |
TW202309018A (en) | Solid state forms of (s)-n-(3-(2-(((r)-1-hydroxypropan-2-yl)amino)-6-morpholinopyridin-4-yl)-4-methylphenyl)-3-(2,2,2-trifluoroethyl)pyrrolidine-1-carboxamide and salts thereof | |
EP3430004A1 (en) | Solid state forms of nilotinib salts | |
US20200216427A1 (en) | Solid state forms of entrectinib | |
WO2022012481A1 (en) | Crystalline or Amorphous form of Bcl-2/Bcl-xL inhibitor compound or its salts | |
KR20220016949A (en) | Polymorphisms of CDK9 inhibitors and their preparation and use | |
Reis et al. | Crystallization of isoniazid in choline-based ionic liquids: Physicochemical properties and anti-tuberculosis activity | |
US10513500B2 (en) | Crystalline forms of Lesinurad | |
US10544129B2 (en) | Crystalline forms of AP26113, and preparation method thereof | |
WO2021000687A1 (en) | Preparation method for crystal form of pac-1 | |
TWI762825B (en) | Crystal form of monomaleate of tyrosine kinase inhibitor and preparation method thereof | |
TWI777438B (en) | Crystalline forms of n- (phenyl sulfonyl) benzamide compounds | |
WO2022171117A1 (en) | Salt of nitrogen-containing fused heterocyclic compound or crystal form thereof, and preparation method therefor, pharmaceutical composition thereof, and use thereof | |
US20230106142A1 (en) | Crystals of alkynyl-containing compound, salt and solvate thereof, preparation method, and applications | |
CN113149998B (en) | Amorphous or crystalline forms of 2-indoline spiroketones or salts, solvates thereof | |
WO2024032615A1 (en) | Crystal form of pyridinopyrimidinone compound, acid salt thereof, crystal form of acid salt thereof, and use | |
WO2023011428A1 (en) | Crystal form of ripk1 inhibitor, acid salt thereof, and crystal form of acid salt thereof | |
CN107935978B (en) | A kind of crystal form of compound and preparation | |
WO2018130226A1 (en) | New crystal form of riociguat, preparation method and use thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 21843042 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 21843042 Country of ref document: EP Kind code of ref document: A1 |