WO2021162168A1 - 아프리카 돼지열병 바이러스 소독제의 제조방법 및 이에 의해 제조되는 아프리카 돼지열병 바이러스 소독제 - Google Patents
아프리카 돼지열병 바이러스 소독제의 제조방법 및 이에 의해 제조되는 아프리카 돼지열병 바이러스 소독제 Download PDFInfo
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- WO2021162168A1 WO2021162168A1 PCT/KR2020/003071 KR2020003071W WO2021162168A1 WO 2021162168 A1 WO2021162168 A1 WO 2021162168A1 KR 2020003071 W KR2020003071 W KR 2020003071W WO 2021162168 A1 WO2021162168 A1 WO 2021162168A1
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- WIPO (PCT)
- Prior art keywords
- swine fever
- african swine
- fever virus
- hypochlorous acid
- acid water
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- 241000701386 African swine fever virus Species 0.000 title claims abstract description 51
- 239000000645 desinfectant Substances 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 80
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- 239000000460 chlorine Substances 0.000 claims abstract description 39
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims abstract description 36
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 36
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- 239000012153 distilled water Substances 0.000 claims abstract description 23
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N29/00—Biocides, pest repellants or attractants, or plant growth regulators containing halogenated hydrocarbons
- A01N29/04—Halogen directly attached to a carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
Definitions
- the present invention relates to a method for producing an African swine fever virus disinfectant and to an African swine fever virus disinfectant prepared thereby.
- African swine fever virus is a first-class livestock contagious disease that occurs in all age groups of wild boars and domestic pigs.
- pigs infected with African swine fever virus show 100% mortality within 2 to 10 days.
- This African swine fever virus was first reported in Kenya in 1921, and started with the first infection in Europe in 1957, and then in China and Southeast Asia. Recently in Korea, efforts are being made to prevent the movement and quarantine of wild boars, which are vectors, in order to block the transmission.
- African swine fever virus is a DNA virus, which is different from the RNA viruses of avian influenza and foot-and-mouth disease. Therefore, a vaccine has not been developed due to the lack of neutralizing antibodies and problems with genetic diversity.
- the main cause of the spread of the African swine fever virus is through contact with infected individuals and the distribution and leftover feeding of infected individuals. It is reported that it can survive, has a long survival period at low temperatures, and is inactivated in strong acids with a pH of 3.9 or less and strong alkalis with a pH of 11.5 or more.
- African swine fever virus disinfectants include citric acid, ortho-phenylphenol, iodine compound, potassium monopersulfate compound, sodium dichlori-s-trizinetrione ( NADCC), sodium hydroxide (NaOH), and aldehyde compounds.
- 'Disinfectant having sterilization and disinfection synergistic effect and manufacturing method thereof (registration number: 10-1440375)', one or more organic acids of formic acid, fumaric acid, malic acid, citric acid, oxalic acid and ascorbic acid, phosphoric acid, boric acid and sulfa Disinfectants for sterilizing livestock pathogens by containing any one or more inorganic acids among the acids have been proposed.
- the present invention was invented to solve the above problems, and a method for producing an African swine fever virus disinfectant comprising sodium-free hypochlorous acid water having a disinfecting effective concentration capable of killing African swine fever virus without cytotoxicity, and It is a technical solution to provide an African swine fever virus disinfectant manufactured by
- the present invention comprises the steps of: preparing an electrolysis product containing chlorine gas by electrolyzing a hydrochloric acid solution into an electrolyzer; and preparing sodium-free hypochlorous acid water while mixing chlorine gas separated from the electrolysis product into distilled water; It provides a method for producing sodium-free hypochlorous acid water for African swine fever virus disinfectants, characterized in that by adjusting the sodium-free hypochlorous acid water having an effective chlorine concentration of 78 ppm or 143 ppm.
- the effective chlorine concentration of the hypochlorous acid water is 78 ppm, it is characterized in that the disinfection treatment by spraying directly on the pig.
- the effective chlorine concentration of the hypochlorous acid water is 143 ppm, it is characterized in that the disinfection treatment by spraying the pig barn.
- the present invention provides a disinfectant for African swine fever virus, characterized in that it comprises sodium-free hypochlorous acid water prepared by the above method.
- the present invention kills the African swine fever virus by directly spraying the African swine fever virus disinfectant to pigs or swine barns, characterized in that the death rate is at least 99.97%, sodium
- a method for killing African swine fever virus using non-containing hypochlorous acid water is provided.
- the manufacturing method of the African swine fever virus disinfectant of the present invention by means of a solution to the above problem, effective chlorine in hypochlorous acid water without sodium through the control of the current supplied to the electrolyzer according to the amount of chlorine gas and the amount of distilled water Since the concentration can be kept constant at 78 ppm or 143 ppm, there is an effect of efficiently performing disinfection of African swine fever virus and preparing a disinfectant that can be used as a virucidal agent without cytotoxicity.
- FIG. 1 is a flow chart according to the manufacturing method of the African swine fever virus disinfectant of the present invention.
- FIG. 1 is a flowchart according to the manufacturing method of the African swine fever virus disinfectant of the present invention.
- a hydrochloric acid solution is added to an electrolyzer and then electrolyzed to produce an electrolytic product containing chlorine gas ( It is prepared through S10) and the step (S20) of preparing sodium-free hypochlorous acid water while mixing chlorine gas separated from the electrolysis product into distilled water.
- sodium-free hypochlorous acid water having an effective chlorine concentration of 78 ppm or 143 ppm is produced by controlling the amount of current supplied to the electrolyzer according to the amount of chlorine gas and the amount of distilled water It is characterized in that, each of the above-described steps can be described as follows.
- a hydrochloric acid solution is put into an electrolyzer and then electrolyzed to prepare an electrolysis product containing chlorine gas (S10).
- electrolysis in general, a pair of electrodes forming a cathode and an anode are installed in an electrolyzer, and electricity is supplied to each electrode to electrolyze the material contained in the electrolyzer.
- electrolysis may be divided into a diaphragm type having a diaphragm between the cathode and an anode, and a diaphragm-free type having no diaphragm depending on the presence or absence of the diaphragm.
- the diaphragm-free type since there is an advantage in that the amount of generated electrolysis products can be increased by increasing the voltage and current and increasing the number of electrodes or the surface area of the electrodes, it is preferable to apply the diaphragm-free electrolysis in the present invention.
- sodium chloride is mixed with a hydrochloric acid solution to further increase the electrolytic ability.
- Sodium chloride has a large ionization, so the ratio of separation into cations and anions during electrolysis is close to 100%, so that the current flows well and the electrode
- the toxicity is so strong that it is inconvenient to use it after diluting it in a large amount of water.
- sodium chloride is mixed with the hydrochloric acid solution It is preferable not to
- sodium-free hypochlorous acid water is prepared while mixing chlorine gas separated from the electrolysis product into distilled water (S20).
- the distilled water introduced for the production of sodium-free hypochlorous acid water is supplied from an adjacent device, the amount of distilled water introduced can always change. Due to this, when the flow rate of distilled water is reduced or increased, the concentration of hypochlorous acid water generated can be increased or decreased.
- the flow rate of the hydrochloric acid solution supplied to the electrolyzer is sensed and the current supplied to the electrolyzer is controlled by controlling the power supply during electrolysis based on the measured value, and generated in the electrolyzer by controlling the current
- the effective chlorine concentration of hypochlorous acid water is maintained at 78 ppm or 143 ppm, so that it can be supplied constantly.
- the current applied to the electrolyzer is controlled so that the effective chlorine concentration of the hypochlorous acid water is maintained at 78 ppm or 143 ppm, it has a certain effective chlorine concentration depending on the required place, so that the disinfecting power of the disinfectant can be constantly maintained.
- the effective chlorine concentration of hypochlorous acid water is adjusted to be 78ppm.
- distilled water is supplied at 1.0 ⁇ 1.5L/min.
- the amount of distilled water is small and the effective chlorine concentration of the final product, hypochlorous acid water, exceeds 78ppm, which is not effective for convection prevention.
- the amount of chlorous acid is too large, and the final product, hypochlorous acid water, is diluted, and the effective chlorine concentration is much lower than 78ppm, so the disinfection efficiency is not good.
- the most preferable supply amount of distilled water is 1.25L/min so that the effective chlorine concentration of hypochlorous acid water becomes 78ppm.
- the effective chlorine concentration of hypochlorous acid water generated by measuring the amount of current supplied to the electrolyzer through the power supply and controlling the power supplied from the power supply based on the measured amount of current is controlled.
- the current is less than 18A, there is a disadvantage that the generation time of hypochlorite water needs to be further increased to meet the 78ppm concentration, and if it exceeds 22A, it is difficult to maintain a constant 78ppm concentration of hypochlorous acid.
- the effective chlorine concentration of the hypochlorous acid water is adjusted to 143ppm.
- hypochlorous acid water having an effective chlorine concentration of 143 ppm
- distilled water is supplied at 0.8 to 0.9 L/min while flowing chlorine gas separated from the electrolysis product. If distilled water is supplied at less than 0.8L/min, the amount of distilled water is small and the effective chlorine concentration of the final product, hypochlorous acid water, exceeds 143ppm, which may increase toxicity.
- the effective chlorine concentration of hypochlorous acid water, which is the final product is much lower than 143ppm, so even if it is sprayed where organic matter is present, the disinfection efficiency is not as good as expected.
- the most preferable supply of distilled water is 0.88L/min so that the effective chlorine concentration of hypochlorous acid water becomes 143ppm.
- the amount of current supplied to the electrolyzer through the power supply is measured, and the power supplied from the power supply is controlled based on the amount of current measured in this way. It controls the effective chlorine concentration of hypochlorous acid water produced by the If the current is less than 28A, there is a disadvantage that the amount of chlorine gas must be increased while increasing the electrolysis degree of the hydrochloric acid solution by increasing the current to meet the 143ppm concentration. There is this. In order to maintain the effective chlorine concentration of hypochlorous acid water at 143ppm, it is most desirable to flow a current of 30A.
- hypochlorous acid water the effect on disinfection power is large depending on the pH.
- it is suitable for application as a disinfectant in the range of pH 5.0 to 6.0, and the sodium-free hypochlorous acid water having an effective chlorine concentration of 78 ppm of the present invention becomes pH 5.5, so it can be an appropriate disinfectant for convection prevention that can be directly sprayed on pigs .
- the pH of hypochlorous acid water is lowered to 4.5 and the effective chlorine concentration is raised to 143 ppm, there is no cytotoxicity, so it is possible to kill the African swine fever virus by spraying it directly into the pig barn.
- electrolysis was performed to prepare an electrolysis product containing chlorine gas.
- 78 ppm of hypochlorous acid water was prepared by controlling a current of 20 A while mixing distilled water at 1.25 L/min under the supply of chlorine gas separated from the electrolysis product. At this time, the pH was 5.5.
- electrolysis was performed to prepare an electrolysis product containing chlorine gas.
- distilled water was mixed at 0.88 L/min and the current was adjusted to 30 A to prepare 143 ppm of hypochlorous acid water.
- the pH of the hypochlorous acid water was 4.5.
- the cytotoxicity and disinfection power of the African swine fever virus disinfectant was tested.
- the inactivation of the virus was determined by finally reading the cytotoxicity of the cells inoculated with the African swine fever virus treated with the disinfectant.
- Table 1 shows the virucidal test conditions for African swine fever.
- Item condition active substance Hypochlorous acid (HOCl) Chlorine concentration of active substance 78ppm, 143ppm Virus processing time 30 minutes Virus test temperature 10°C Interfering substance (organic molar) concentration high concentration BSA 10g/L + yeast extract 10g/L low concentration BSA 3g/L additive-free MEM + 7% FCS cell culture temperature 37°C Virus species used African Swine Fever Virus (ASFV) strain BA71V
- disinfectants having effective chlorine concentrations of 78 ppm and 143 ppm were prepared according to Examples 1 and 2 according to the African swine fever virus efficacy test method, respectively, and the African swine fever virus used in the experiment was African Swine Fever Virus (ASFV) strain BA71V was used.
- Experimental treatment temperature was 10°C for 30 minutes.
- the experimental group was divided into 3 groups, and for each of the experiments to verify the efficacy when organic substances were added, 3g/L bovine serum albumin was added to those containing low concentrations of organic substances, and high concentrations of organic substances were added.
- 10g/L bovine serum albumin and 10g/L yeast extract were used together.
- efficacy experiments were also conducted in the cell culture medium alone addition group.
- the experimental method for verifying the virucidal virus for African swine fever was carried out in the same manner as in Table 2 below.
- the experimental method as shown in Table 2, first, 1 ml of an organic material to be introduced as an interfering material was put into an experimental container, and 1 ml of a virus culture solution was added.
- the experimental vessel is a thermostat maintained at 10 ⁇ 1°C, and the organic material and the virus culture solution were mixed for 2min ⁇ 10s. Then, 8ml of the disinfectant test solution was added and mixed, and treated at 10 ⁇ 1°C for 30min ⁇ 10s. Mix well just before the end of the treatment time.
- test solution 0.5 ml was added to 4.5 ml of a mixture of MEM (minimum essential medium) + 2% FCS (fetal calf serum) maintained in a thermostatic water bath maintained at 4 ⁇ 1°C or crushed ice. added.
- MEM minimum essential medium
- FCS fetal calf serum
- test substance is diluted to 10 -9 with a mixture of MEM (minimum essential medium) + 2% FCS (fetal calf serum) maintained in a constant temperature bath maintained at 4 ⁇ 1°C or crushed ice did.
- MEM minimum essential medium
- FCS fetal calf serum
- the experimental dilutions after virus treatment were stored at 4°C or under crushed ice until inoculated into cell cultures.
- the effective chlorine concentration of the disinfectant used in the experiment was tested at 78 ppm, and this will be denoted as MCC-A.
- concentration of MCC-A actually used in the experiment was tested at the final 80% level according to the addition of virus solution, etc., and the treatment conditions were treated at 10° C. for 30 minutes.
- Item density organic matter Cell cultured cells 50% infection concentration (lg TCID50) Difference between virus control and experimental group (log10) MCC-A 80% bovine albumin 10g/L+ yeast extract 10g/L 5.5 0.5 MCC-A 80% bovine albumin 3g/L 2.5 3.5 MCC-A 80% - ⁇ 1.5 ⁇ 4.5 Virus control N/a bovine albumin 10g/L+ yeast extract 10g/L 6 N/a Virus control N/a bovine albumin 3g/L 6 N/a Virus control N/a - 6 N/a N/a; not applicable
- the high concentration organic matter addition group has a value of 0.5 log10, which means that when MCC-A is sprayed as a stock solution, there is a virucidal effect of 50%.
- the low-concentration organic substance addition group it is 0.5441 when converted to a constant log value with a value of 3.5 log10.
- the experimental group where no organic matter was added it is 0.6532 when converted to a constant log value with a value of ⁇ 4.5 log10.
- the virucidal effect of 80% concentration of MCC-A under low concentration organic matter was 3.5 as a log value through comparison with the control group. Through this, it can be confirmed that it has a bactericidal effect with an approximately 5,400-fold dilution and has 99.98% virucidal power.
- 80% MCC-A was checked for disinfection in the absence of organic matter, and the results are as follows. Table 7 shows.
- Table 7 shows the results of the disinfection power test of 80% MCC-A in the absence of organic matter.
- the virucidal effect is a log value through comparison with the control. ⁇ 4.5. Converting this to a constant log value, it is 0.6532, which means that there is a virucidal effect with a 6,500-fold dilution, so it has 99.99% virucidal power.
- a secondary disinfection test was conducted to verify the virucidal virus of MCC-A against African swine fever.
- the laboratory was conducted at the Polish National Academy of Veterinary Sciences in the same way as the first experiment.
- the test procedure was performed according to "5.7 virucidal test" of PN-EN-14675_2015-06E, a European standard test regulation.
- the effective chlorine concentration of MCC-A used in the experiment was tested at 143 ppm, and this will be referred to as MCC-A1.
- the concentration of MCC-A1 actually used in the experiment was tested at the final 80% level according to the addition of virus solution. Treatment conditions were treated at 10° C. for 30 minutes.
- 10g/L BSA + 10g/L yeast extract was added in the high concentration organic matter treatment condition, and 3g/L BSA was added in the low concentration organic matter treatment condition. used in the experiment.
- the cytotoxicity of MCC-A1 on the morphology and growth of cells by comparison with the control group for each experimental group was also conducted.
- the virus, culture medium, and toxicity according to the experimental method were performed, and it was confirmed that the test substance, MCC-A1, had no toxicity at all like MCC-A.
- the results of the secondary virus efficacy test the results of the virucidal secondary efficacy test of the disinfectant having an effective chlorine concentration of 143 ppm were shown.
- Item density organic matter Cell cultured cells 50% infection concentration (lg TCID50) Difference between virus control and experimental group (log10) MCC-A1 80% bovine albumin 10g/L+ yeast extract 10g/L 5.5 2.25 MCC-A1 80% bovine albumin 3g/L 2.5 4.25 Virus control N/a bovine albumin 10g/L+ yeast extract 10g/L 7.25 N/a Virus control N/a bovine albumin 3g/L 6.75 N/a
- Table 9 shows the results of the secondary efficacy test for virucidal MCC-A1, and it can be seen that Table 9 shows the results of the efficacy test for the African swine fever virus of MCC-A1 at a concentration of 143ppm.
- Table 9 shows the results of the efficacy test for the African swine fever virus of MCC-A1 at a concentration of 143ppm.
- MCC-A1 at a concentration of 143ppm it is 0.3522 when converted to a constant log value as 2.25 log10 in the high concentration organic matter addition group, which has a virucidal effect with an approximately 3,500-fold dilution, and has a virucidal effect of 99.97%.
- the virucidal effect of MCC-A1 under high-concentration organic matter was 2.25 as a log value through comparison with the control, and when converted to a constant log value, it was 0.3522, about 3,500 times dilution. It can be confirmed that it has a virucidal power of 99.97%. Then, 3 g/L of BSA was added to MCC-A1 of 80% concentration to create low-concentration organic matter treatment conditions, and then the disinfection power was tested, and the results are shown in Table 11 below. It was.
- Table 11 is the experimental data of the disinfecting power effect of 80% MCC-A1 against 3g/L BSA. As shown in Table 11, the virucidal effect of MCC-A1 under low-concentration organic matter was compared with the control, and the log value was 4.25. appears, and when converted to a constant log value, it is 0.6284, which has a virucidal effect with an about 6,200-fold dilution, and it can be seen that it has 99.99% virucidal power.
- Sodium-free hypochlorous acid water through the invention has not only no cytotoxicity, but also has more than 99.97% virucidal power against African swine fever, thereby confirming that it can kill the deadly viral hemorrhagic swine epidemic virus.
- the present invention can produce sodium-free hypochlorous acid water that maintains an effective chlorine concentration of 78 ppm or 143 ppm constant by adjusting the amount of current supplied to the electrolyzer according to the amount of chlorine gas and the amount of distilled water. have.
- the virucidal virus experiment on African swine fever attempted in the experimental example of the present invention has not been attempted before, and the effective chlorine concentration of hypochlorous acid water, which is the main effective substance, is adjusted to 78 ppm or 143 ppm to be used as a disinfectant for African swine fever virus. It is expected that the value of the present invention can be recognized in that it presents a new proposal for the application of
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Abstract
Description
항목 | 조건 | |
유효물질 | 차아염소산(HOCl) | |
유효물질의 염소농도 | 78ppm, 143ppm | |
바이러스 처리시간 | 30분 | |
바이러스 실험온도 | 10℃ | |
방해물질(유기몰) 농도 | 고농도 | BSA 10g/L + yeast extract 10g/L |
저농도 | BSA 3g/L | |
무첨가군 | MEM + 7% FCS | |
세포배양 온도 | 37℃ | |
사용된 바이러스 종 | African Swine Fever Virus(ASFV) strain BA71V |
순서 | 내용(유럽 표준형: PN-EN-14675_2015-06E "5.7 virucidal test") |
1 | 유기물 1ml + 바이러스 배양액 1ml |
2 | 10±1℃ 유지되는 항온조에서 2min ± 10s 간 혼합 |
3 | 실험용액 8ml을 첨가 혼합하고,10±1℃에서 30 min ± 10 s 간 처리 |
4 | 실험액 0.5ml을 4±1℃유지한 MEM(minimum essential medium) +2 % FCS(fetal calf serum; 소태아 혈청) 혼합액 4.5ml에 첨가 |
5 | 4±1℃ 유지한 MEM + 2% FCS 혼합액으로실험물질이 10-9까지 희석된 실험 표본을 준비 |
6 | 실험 희석액은 세포배양에 접종할 때까지 4℃ 유지 |
7 | 실험 희석액을 배양세포가 함유된 배양 용기 단위로 접종 |
8 | 각 희석액은 8개 단위별로 접종 |
9 | 세포배양 후 희석별로 바이러스 감염도 측정 |
10 | 정량적 실험의 적정 결과는 희석 배율에 따른 CPE(cytopathic effect; 세포병변효과)의 존재 유무에 따라 10~100% 비율로 측정[Spearman and Karber 의 방법에 따라 측정] |
11 | Plaque assay(바이러스의 존재 유무 확인법)도 병행하여 수행 |
12 | 각 실험구 및 대조구의 세포변성 및 세포성장 측정 등을 통하여 세포독성 판별 |
조항 | 수행 여부 |
실험 바이러스 현탁액의 적정(실험물질의 처리 전후 로그수치 4 감소의 결정에 따른다.) | 수행 |
실험물질의 세포독성이 없음의 판별 여부(세포의 변성, 증식 또는 바이러스 감수성) | 수행 |
배양세포에 접종된 바이러스 비교 검토(실험 혼합액 또는 배양배지의 희석에 따른 비교) | 수행 |
항목 | 농도 | 유기물 | 세포배양세포50% 감염농도(lg TCID50) | 바이러스 대조구와실험구의 차이값(log10) |
MCC-A | 80% | bovine albumin 10g/L+ yeast extract 10g/L | 5.5 | 0.5 |
MCC-A | 80% | bovine albumin 3g/L | 2.5 | 3.5 |
MCC-A | 80% | - | ≤1.5 | ≥4.5 |
Virus control | N/a | bovine albumin 10g/L+ yeast extract 10g/L | 6 | N/a |
Virus control | N/a | bovine albumin 3g/L | 6 | N/a |
Virus control | N/a | - | 6 | N/a |
N/a; not applicable |
항목 | 농도 | 유기물 | 처리시간(분) | 희석농도 (lg) | |||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
MCC-A | 80% | bovine serumalbumin 10g/L+yeast extract10g/L | 30 | 1111 | 1111 | 1111 | 1111 | 0000 | 0000 | 0000 | 0000 |
Viruscontrol | N/a | bovine serumalbumin 10g/L+yeast extract10g/L | 30 | 1111 | 1111 | 1111 | 1111 | 1010 | 0000 | 0000 | 0000 |
1; virus present - 4개의 세포배양내에서의 세포변성효과(CPE)0; no virus presentN/a; not applicable |
항목 | 농도 | 유기물 | 처리시간(분) | 희석농도 (lg) | |||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
MCC-A | 80% | bovine serum albumin3g/L | 30 | 1011 | 0010 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 |
Viruscontrol | N/a | bovine serum albumin3g/L | 30 | 1111 | 1111 | 1111 | 1111 | 0011 | 0000 | 0000 | 0000 |
1; virus present - 4개의 세포배양내에서의 세포변성효과(CPE),0; no virus presentN/a; not applicable |
항목 | 농도 | 유기물 | 처리시간(분) | 희석농도 (lg) | |||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
MCC-A | 80% | None | 30 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 |
Viruscontrol | N/a | None | 30 | 1111 | 1111 | 1111 | 1111 | 1001 | 0000 | 0000 | 0000 |
1; virus present - 4개의 세포배양내에서의 세포변성효과(CPE)0; no virus presentN/a; not applicable |
조항 | 수행 여부 |
실험 바이러스 현탁액의 적정(실험물질의 처리 전후 로그수치 4 감소의 결정에 따른다.) | 수행 |
실험물질의 세포독성이 없음의 판별 여부(세포의 변성, 증식 또는 바이러스 감수성) | 수행 |
배양세포에 접종된 바이러스 비교 검토(실험 혼합액 또는 배양배지의 희석에 따른 비교) | 수행 |
항목 | 농도 | 유기물 | 세포배양세포 50% 감염농도(lg TCID50) | 바이러스 대조구와 실험구의 차이값(log10) |
MCC-A1 | 80% | bovine albumin 10g/L+ yeast extract 10g/L | 5.5 | 2.25 |
MCC-A1 | 80% | bovine albumin 3g/L | 2.5 | 4.25 |
Virus control | N/a | bovine albumin 10g/L+ yeast extract 10g/L | 7.25 | N/a |
Virus control | N/a | bovine albumin 3g/L | 6.75 | N/a |
항목 | 농도 | 유기물 | 처리시간(분) | 희석농도 (lg) | |||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
MCC-A1 | 80% | bovine serum albumin10g/L+yeast extract10g/L | 30 | 1111 | 1111 | 1111 | 1111 | 0000 | 0000 | 0000 | 0000 |
Viruscontrol | N/a | bovine serum albumin10g/L+yeast extract10g/L | 30 | 1111 | 1111 | 1111 | 1111 | 1111 | 1010 | 0010 | 0000 |
1; virus present - 4개의 세포배양내에서의 세포변성효과(CPE)0; no virus presentN/a; not applicable |
항목 | 농도 | 유기물 | 처리시간(분) | 희석농도 (lg) | |||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
MCC-A1 | 80% | bovine serum albumin 3g/L | 30 | 1111 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 | 0000 |
Viruscontrol | N/a | bovine serum albumin 3g/L | 30 | 1111 | 1111 | 1111 | 1111 | 1111 | 0100 | 0000 | 0000 |
1; virus present - 4개의 세포배양내에서의 세포변성효과(CPE)0; no virus presentN/a; not applicable |
Claims (5)
- 염산용액을 전해조에 투입한 후 전기분해하여 염소가스를 포함하는 전해생성물을 제조하는 단계; 및상기 전해생성물로부터 분리된 염소가스를 증류수에 혼입하면서 나트륨 미함유 차아염소산수를 제조하는 단계;를 포함하여 이루어지고,상기 염소가스의 양과 상기 증류수의 양에 따라 상기 전해조에 공급되는 전류의 양을 조절함으로써 유효 염소농도가 78ppm 또는 143ppm인 나트륨 미함유 차아염소산수를 제조하는 것을 특징으로 하는,아프리카 돼지열병 바이러스 소독제용 나트륨 미함유 차아염소산수의 제조방법.
- 제1항에 있어서,상기 차아염소산수의 유효 염소농도가 78 ppm이고, 돼지에 직접 분사하여 소독처리하는 것을 특징으로 하는,아프리카 돼지열병 바이러스 소독제용 나트륨 미함유 차아염소산수의 제조방법.
- 제1항에 있어서,상기 차아염소산수의 유효 염소농도가 143 ppm이고, 돼지 축사에 분사하여 소독처리하는 것을 특징으로 하는,아프리카 돼지열병 바이러스 소독제용 나트륨 미함유 차아염소산수의 제조방법.
- 제1항 내지 제3항 중 어느 한 항의 방법으로 제조되는 나트륨 미함유 차아염소산수를 포함하여 이루어지는 것을 특징으로 하는, 아프리카 돼지열병 바이러스 소독제.
- 제4항에 따른 아프리카 돼지열병 바이러스 소독제를 돼지 또는 돼지 축사에 직접 분사하여 아프리카 돼지열병 바이러스를 사멸하되, 사멸율이 적어도 99.97%인 것을 특징으로 하는, 나트륨 미함유 차아염소산수를 이용한 아프리카 돼지열병 바이러스의 사멸방법.
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