WO2021104499A1 - Sample adding and mixing method and apparatus, and non-transitory computer readable storage medium - Google Patents
Sample adding and mixing method and apparatus, and non-transitory computer readable storage medium Download PDFInfo
- Publication number
- WO2021104499A1 WO2021104499A1 PCT/CN2020/132465 CN2020132465W WO2021104499A1 WO 2021104499 A1 WO2021104499 A1 WO 2021104499A1 CN 2020132465 W CN2020132465 W CN 2020132465W WO 2021104499 A1 WO2021104499 A1 WO 2021104499A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- mixing
- setting part
- placement
- suspended
- Prior art date
Links
- 238000002156 mixing Methods 0.000 title claims abstract description 110
- 238000000034 method Methods 0.000 title claims abstract description 66
- 238000012546 transfer Methods 0.000 claims abstract description 77
- 238000012360 testing method Methods 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims description 88
- 239000003153 chemical reaction reagent Substances 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 29
- 230000008569 process Effects 0.000 claims description 11
- 238000004590 computer program Methods 0.000 claims description 10
- 239000000654 additive Substances 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 8
- 239000000126 substance Substances 0.000 abstract description 6
- 239000000758 substrate Substances 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 230000015271 coagulation Effects 0.000 description 8
- 238000005345 coagulation Methods 0.000 description 8
- 239000012503 blood component Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 230000010100 anticoagulation Effects 0.000 description 5
- 230000020764 fibrinolysis Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 238000009534 blood test Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000010230 functional analysis Methods 0.000 description 2
- 238000002558 medical inspection Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000012284 sample analysis method Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 238000003805 vibration mixing Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- -1 etc. Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/712—Feed mechanisms for feeding fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/7174—Feed mechanisms characterised by the means for feeding the components to the mixer using pistons, plungers or syringes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
Definitions
- This application relates to the technical field of medical inspection, and specifically relates to a sample adding and mixing method and device, and a non-temporary computer-readable storage medium.
- this application develops a sample adding and mixing method that can effectively shorten the time from the mixing link to the detection link. At the same time, it also provides a sample adding and mixing device that can realize the above method, which is non-temporary.
- Computer readable storage medium
- One of the technical means adopted in this application is to provide a sample adding and mixing method, which includes the following steps: placing the sample placing part on a suspended position; adding the sample adding part to the sample placing part; Before the sample portion reaches the detection position, the sample placement portion is mixed by the transfer portion, so that the additives in the sample placement portion are mixed uniformly to obtain the test object.
- a sample adding and mixing device comprising: a sample adding part and a transplanting part, wherein the sample adding part is used when the sample placing part is in a suspended position, Add objects to be added to the sample setting part; the transfer part is used to move the sample setting part from the placing position to the suspended position, and perform a mixing operation on the sample setting part to make the sample setting part The additives in the part are mixed to obtain the test object, and the sample placement part is moved to the detection position to detect the test object; wherein, the distance between the placement position and the detection position Greater than the distance between the suspended position and the detection position.
- Another technical means adopted in this application is to provide a non-transitory computer-readable storage medium, wherein the non-transitory computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, The processor executes the following steps: placing the sample placing part in a suspended position through the transplanting part; adding the sample adding part to the sample placing part; before the sample placing part reaches the detection position, by transferring The part performs a mixing operation on the sample setting part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.
- the sample setting part is first placed in a suspended position, and then the object to be added is added to the sample setting part, and before the sample setting part reaches the detection position, the sample setting part is mixed by the transfer part. So that the additives in the sample setting part are mixed evenly. Therefore, there is no need to wait for the sample setting part to perform the mixing operation after reaching the detection position, thereby shortening the time from the mixing step to the detection step.
- Figure 1 is a flow chart of a sample adding and mixing method in an embodiment
- Figure 2 is a flow chart of a sample adding and mixing method in an embodiment
- Figure 3 is a flow chart of a sample adding and mixing method in an embodiment
- Fig. 4 is a flow chart of the steps of the sample adding part adding sample to the sample placing part in an embodiment
- Figure 5 is a partial flow chart of a sample adding and mixing method in an embodiment
- Fig. 6A, Fig. 6B, Fig. 6C, Fig. 6D are diagrams of implementation examples of the sample adding and mixing method
- Figure 7 is a partial flow chart of a sample adding and mixing method in an embodiment
- Figure 8 is a diagram of an implementation example of moving the sample setting part to the detection position
- Figure 9 is a partial flow chart of a sample adding and mixing method in an embodiment
- Figure 10 is a diagram showing an example of an implementation of moving the sample setting part from the placement position to the suspended position
- Figure 11 is a structural block diagram of a sample adding and mixing device in an embodiment
- Figure 12 is a structural block diagram of a sample adding and mixing device in an embodiment
- Fig. 13 is a structural block diagram of a non-transitory computer-readable storage medium in an embodiment.
- sample adding method may include the following steps:
- Step S12 Place the sample setting part in a suspended position.
- the sample setting part when the sample setting part is in a suspended position, the sample setting part is in a suspended state.
- the sample placement part refers to a container in which samples or reagents can be placed, and it can be a sample container, a reagent container, such as a reaction cup, a test tube, and the like.
- the specific type and form of the sample setting part have no influence on the sample adding and mixing method claimed in this application.
- the suspended state of the sample placing part means that the bottom of the sample placing part is not placed on any bearing surface, and the sample placing part in the suspended position can be clamped or fixed by the transfer part to keep the sample placing part suspended.
- the transfer part may be a part or structure with a fixing function or a clamping function such as a manipulator, a finger cylinder, a gripper, etc.; in one embodiment, the suspended position may be located above the placement position, above the detection position, or between the placement position and the detection position. between.
- the placement position may be the position where the sample placement part is placed before being placed in the suspended position.
- the placement position can be a position for placing an empty sample placement part, or it can be used as a pre-sampling position, that is, first add one or part of a sample or reagent to the sample placement part at the placement position.
- the sample placement part where the sample or reagent has been added to the pre-loading position can also be incubated before moving to the suspended position.
- the suspended position can be a position that is convenient for the sample application part to reach and move the sample setting part to the detection position.
- the selection of the suspension position can be based on the distance between the suspended position and the detection position, the distance between the suspended position and the initial position of the sampling part, and the suspended position
- the distance from the initial position of the sample setting part is determined, specifically, it may be the suspended position corresponding to the case where the sum of the above three distances is the shortest.
- the detection position can be a position where the sample setting part can be placed and the test object in the sample setting part can be detected.
- the test object can be the object to be added by the sample adding part in the following step S14, or it can be in the following step S14
- the samples and reagents placed in the pre-sample section may also be substances obtained by reacting the object to be added with other samples and reagents in the pre-sample section.
- Step S14 the sample adding part adds the object to be added into the sample placing part.
- the sample adding part can be a sample needle, or other parts capable of aspirating samples or reagents.
- the substance to be added by the sample adding part to the sample placing part can be only the sample, or only the reagent, or both the sample and the reagent are added. If only the sample is added, the reagent needs to perform the following step S16 Add at the time or before. If only the reagent is added, the sample needs to be added before step S14. For example, the sample can be added to the sample setting part in the setting area of the sample setting part, and then the sample setting part can be moved to the suspension position.
- the sample setting part moves in the front and the sample application part is loaded after is compared to the method where the sample setting part is moved before and the sample setting part moves behind, which reduces the movement of the sample setting part that has already been loaded. Time helps reduce the occurrence of sample contamination.
- the sample can be blood-related samples, such as blood, blood components, etc., blood components can be plasma, blood cells, etc.; reagents can be fibrinolysis, antifibrinolysis, coagulation, and anticoagulation And other detection reagents.
- sample adding section can add objects to be added to the sample placing section in a moving state, or add objects to be added to the sample placing section in a suspended state, that is, when the sample adding section adds objects to be added to the sample placing section, the two People can be in relative motion or relatively static state.
- Step S16 Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly.
- the sample setting part has at least one of a moving state and a mixing state.
- the transfer part can be a part or structure with a clamping function, such as a manipulator, a finger cylinder, or a clamping jaw. It can drive the sample setting part to move, for example, transfer the sample setting part to the suspended position, transfer to the detection position, or drive the sample setting part to be in a state of mixing, and then face the objects to be added in the sample setting part. Shaking is performed, for example, the sample and/or reagent are mixed, for example, the blood-related sample and the detection reagent can be shaken.
- the sample placement part can be in both a moving state and a mixing state, for example, mixing while moving.
- the sample setting part can also be kept in the suspended position for a predetermined time, and the predetermined time can be the time required to implement step S14 or step S16.
- the position where the sample setting part is in the mixing state may be a suspended position, or any position that the sample setting part can pass during the process of moving from the suspended position to the detection position.
- the specific mixing position of the sample setting part can be the suspended position.
- the sample addition part also directly adds the substance to be added to the sample setting part located in the suspended position, and directly adjusts the sample in the suspended position after the sample is added.
- the sample setting part performs a mixing operation to mix the additives in the sample setting part evenly.
- the position where the sample setting part is specifically mixed can be any position that can be passed during the process of moving the sample setting part from the suspended position to the detection position, that is, the sample setting part can be transferred from the suspended position to the detection position in the transfer part During the process of mixing the sample setting part, there can be multiple moving paths from the suspended position to the detection position, as long as the sample setting part can be moved from the suspended position to the detection position.
- the sample setting part can keep moving during the moving process, of course, it can also pause after moving a certain path, and then continue to enter the moving state after pausing for a certain period of time.
- the moving path and the state of the sample setting part during the moving process can be set according to requirements.
- the sample setting part can reach the detection position through moving path 1 from the suspended position, or reach the detection position through moving path 2, or reach the detection position through other moving paths. Any point on any moving path can be opposite.
- the position where the sample part performs the mixing operation of course, this position may be above the detection position where the sample part can be accommodated.
- the implementation position of the mixing operation can be determined according to the best detection time of the object to be added this time.
- the sample setting part clamped by the transfer part can be used to apply a driving force that enables the sample setting part to perform the mixing action.
- the driving force includes at least one of a rotation force, a swing force and a vibration force
- the sample setting part realizes rotation mixing, swing mixing, vibration mixing, or other mixing actions.
- the rotational force, swing force, and vibration force applied by the transfer part can appear superimposed or appear in sequence, for example, the sample placement part sometimes performs rotation mixing and sometimes vibration mixing.
- the duration of the mixing action can also be adjusted according to the actual situation.
- the mixing mechanism is set in the placement area of the sample placement part. After the sample and reagent loading operations are completed, they are directly mixed at the placement location, and then the sample is detected at the detection location. Since the sample placement part needs to move from the placement area to the detection position after mixing, it takes a long time and it is easy to miss the optimal detection time of the sample, especially for the quick response sample detection process. This mixing method has an adverse effect on the inspection speed and performance.
- the other is that the mixing mechanism is set at the detection position of the sample, and the sample and reagent are transferred to the detection position after the sample and reagent addition operation is completed, and the mixing is performed at the detection position.
- the structure of this mixing method is relatively complicated, and for the unified detection and analysis of multiple samples, a mixing mechanism needs to be added to each detection channel, which significantly increases the cost.
- the sample placing part is first placed in a suspended position, and then the object to be added is added to the sample placing part, and then the sample placing part is mixed by the transfer part.
- the position where the sample setting part is mixed can be the position where the sample setting part is in the mixing state, such as a suspended position, or any position that the suspended position can pass during the process of moving to the detection position, so that the sample addition and mixing method can be realized.
- An additional mixing mechanism is provided in the placement area or detection position of the sample placement part, which can effectively reduce costs; at the same time, the sample placement part located in the suspended position is mixed and moved after sample addition, which is convenient to reduce the movement of the sample placement part to the detection position. Time, also avoids the problem of missing the best detection time after mixing, which is beneficial to improve the detection efficiency and the accuracy of the detection results.
- sample analysis equipment such as chemiluminescence analyzers, coagulation analyzers and other clinical testing equipment
- it can effectively avoid the problem of missing the best sample analysis and detection time on the basis of cost control, which is conducive to obtaining More accurate sample analysis results.
- the position where the sample setting part is in the mixing state is a suspended position, or a position between the suspended position and the detection position, because the sample setting part is suspended and mixed, it can also effectively avoid the sample setting part and its bearing surface. The problem of collision.
- the sample adding and mixing method may further include the following steps:
- Step S20 The transfer part moves the sample setting part to the detection position.
- step S20 can be performed after step S16, that is, the sample setting part transferred to the detection position has completed the operations of adding the object to be added and mixing the object to be added, then the sample setting part is directly Transfer to the detection position for sample detection.
- step S20 can also be performed between step S14 and step S16, that is, the sample placement part that has been transferred to the detection position has passed the sample addition of the object to be added but has not performed the mixing operation, and is transferred to After detecting the position, firstly mix the sample placement part through the transfer part, and then perform the sample detection.
- the two methods can be selected according to the actual sample reaction and analysis requirements.
- the method for adding and mixing samples may further include the following steps:
- Step S32 Detect whether the sample setting part is placed on the transfer part.
- a sensor a sensing element, a detection component, etc. can be used to detect whether the sample placement part is clamped on the transfer part.
- Step S34 When the sample setting part is placed on the transfer part, the current position of the sample setting part is acquired.
- the sample setting part is currently located in the suspended position, the detection position, etc.
- the current position of the sample setting part can be obtained by detecting the specific position of the transfer part holding the sample setting part.
- the transfer part can be controlled to perform the clamping operation of the sample setting part.
- Step S36 According to the current position of the sample setting part, the state of the sample setting part is controlled by the transfer part.
- the transfer part can be used to control the sample setting part to be in a moving state.
- the sample setting part can be controlled to be in a suspended state, and at the same time, the sample adding part can be controlled to perform the sample adding work. After a predetermined time, the sample setting part can be controlled to enter the mixed position. Uniform state or mobile state, etc.
- the step S14 of adding the object to be added by the sample adding part to the sample placing part may include:
- Step S142 the sample adding part and the sample setting part are close to each other.
- the sample setting part before sample addition can be an empty sample setting part, that is, before sample addition, there is no liquid such as sample or reagent in the sample setting part, and the sample setting part before sample addition can also be filled with liquid in advance, that is, to the existing liquid Add samples or reagents to the sample setting section.
- the position of the sample setting part can be a suspended position; you can also control the sample setting part to move to the sample part, and you can also control the sample adding part and the sample setting part. Both move towards each other.
- Step S144 the sample adding part adds the object to be added to the sample placing part at the sample adding position.
- the sample adding position refers to the position where the sample adding part is when adding the object to be added, and the sample adding position can be set in advance.
- the objects to be added can be samples, reagents, etc., if the sample adding part is a sample needle, the objects to be added can be discharged from the sample ejection port of the sample needle.
- Step S146 After the sample application is completed, the sample application part and the sample setting part are far away from each other.
- the sample adding part 5 moves to the sample placing part 2 placed on the suspended position 3 to add samples.
- the first state or the second state may be satisfied between the sample adding portion 5 at the sample adding position and the liquid level in the sample placing portion 2.
- the first state is that the liquid contact position of the sample adding section 5 just touches the liquid surface in the sample placing section 2, that is, the two are at the same height level;
- the second state is that the liquid contact position of the sample adding section 5 is located at the sample placing section At a certain distance above the liquid surface in 2 and the distance from the liquid surface in the sample placement part 2 is less than a preset distance.
- the liquid contact position of the sample adding part 5 is located above the liquid surface 7 in the sample placing part 2 and the distance from the liquid surface 7 in the sample placing part 2 If the distance is less than the preset distance, the liquid level 7 in the sample adding portion 5 and the sample placing portion 2 is in the second state; as shown in Fig. 6C, the sample adding portion 5 touches the liquid after the object to be added is discharged.
- the position just touches the liquid surface 7 in the sample setting part 2, that is, the liquid surface 7 in the sample setting part 2 is in the first state between the sample application part 5 and the liquid surface 7 in the sample setting part 2.
- the liquid contact position of the sample adding part can be any position between the front end of the sample adding part and the discharge port of the sample adding part, and the front end is close to the discharge port. Specifically, it can be the front end, the discharge port, or the front end. And the middle position between the sample port and so on.
- the preset distance can be the height of a naturally falling sample drop formed when the sample dispensing part performs the sample discharge operation.
- the preset distance can be obtained through prior experiments.
- the sample dispensing part is used to perform the sample drop operation, and the formation is obtained by measurement.
- the height value of a sample droplet that falls naturally can be used as the value of the preset distance.
- the preset distance can take a value of 0 to 1 mm, for example, 0 mm, 0.5 mm, and 1 mm.
- the sample droplet formed on the sample loading part can be brought into contact with the liquid surface in the sample loading part, thereby achieving viscous force between the sample droplet and the liquid surface in the sample loading part, and at the same time
- the surface tension of the liquid in the sample placement part will also have an effect on the sample droplets.
- the liquid viscosity and surface tension in the sample placing part, as well as the gravity of the sample droplet itself fall into the sample placing part, which avoids the occurrence of sample hanging and residue, and improves
- the accuracy of sample addition can improve the accuracy of sample analysis results when applied to sample analysis.
- the sample adding and mixing method may further include the following step: moving the sample adding part above the sample placing part.
- the sample application part After the sample application part has absorbed the sample, it can form an upper and lower positional relationship with the sample setting part in the vertical direction. Specifically, the sample application part can be moved above the sample setting part.
- the sample setting part can also be When the operation is placed in the suspended position, directly move the sample setting part below the sample application part, then the execution of this step can be omitted.
- the step of approaching the sample application part to the sample placement part and the step of placing the sample placement part in a suspended position can be performed at the same time.
- the initial position of the sample loading part is set so that it also needs to move to the vicinity of the suspended position, in order to save the time of part transfer, you can simultaneously control the movement of the sample loading part while performing the operation of placing the sample loading part in the suspended position, so that It moves to above the sample placement part placed in the suspended position.
- the step of placing the sample placement part in the suspended position may be: the transfer part moves the sample placement part from the placement position to the suspended position.
- the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position, and moving from the suspended position to the detection position can save the moving path of the transfer part and reduce the movement time of the transfer part.
- the placement position can be the placement area of the sample placement section, for example, multiple placement sections are placed in the placement section box located on the placement area, or the placement section is placed on the placement area, and the placement area provides a bearing for the placement section.
- the sample setting part usually performs the sample adding operation in the placing area, and after the mixing is completed, the sample setting part is moved to the detection position, which takes a lot of time. As shown in FIG. 6A, the sample setting part 2 can be moved from the placement position 1 to the suspended position 3 first, and then from the suspended position 3 to the detection position 4.
- the placing and moving operation of the sample setting part can be carried out through the transfer part.
- the transfer part clamps and grabs the sample setting part, and drives the sample setting part to move. After reaching the corresponding position, if there is no supporting surface at the position, such as the above-mentioned suspended position, the transfer part holds and fixes the sample setting part Keep it at the corresponding position. If there is a supporting surface at the position, such as the detection position, the transfer part releases the sample placement part and places it in the corresponding position.
- the sample placement part 2 placed on the detection position 4 has completed the sample addition and mixing operations at this time, and the detection operations of the analyte, such as the mixed sample and/or reagent, can be performed.
- the step S20 of moving the sample setting part to the detection position by the transfer part may include:
- the transfer part 6 is switched from the state of holding and fixing the sample placing part 2 in the suspended position 3 to the state of driving the sample placing part 2 to move, and moves from the suspended position 3 to the detection position 4, and After reaching the detection position 4, the sample setting part 2 is released, and the movement process is smooth and smooth.
- the step of moving the sample placement part from the placement position to the suspended position by the transfer part may include:
- Step S41 the transfer part clamps the sample placement part located at the placement position
- Step S42 The transfer part transfers the sample setting part to the suspended position.
- the transfer part 6 clamps the sample part 2 and moves from the placement position 1 to the suspended position 3.
- the application also provides a sample analysis method, which includes the sample adding and mixing method of any one of the foregoing embodiments.
- the sample analysis method can be applied to a coagulation analyzer to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation.
- the sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.
- the sample adding and mixing device may include: a sample adding portion 11, a first sample adding driving portion 12, and a second sample adding portion.
- the sample adding part 11 is used for sucking the object to be added and adding the sample into the sample placing part, which can be a sample needle;
- the first sample adding driving part 12 is used for driving the movement of the sample adding part 11;
- the first sample adding driving 12 can be It is a power element such as an XYZ three-axis motion platform, a rotary motion platform, and a motor.
- the transfer part 14 shared with the sample setting part;
- the second sample adding driving part 13 is used to drive the sample adding part 11 to realize the adding The operation of sucking or discharging the substance;
- the second sample feeding driving part 13 can be a syringe; the working principle of the syringe can be that the plunger in the syringe moves up and down under the drive of the driving motor, which causes the volume of the sealed cavity to change to form different vacuum degrees.
- the second sample adding driving part 13 is connected to the sample adding part 11 and can be used to drive the sample adding part 11 to realize the suction or discharge operation of the sample or the reagent.
- the transfer part 14 is provided with a clamping mechanism for clamping and releasing the sample setting part 11 and a mixing mechanism for mixing the sample setting part; the control part 15 is used for controlling the first sample driving part 12 and the second
- the sample loading drive unit 13 and the transfer unit 14 are controlled, and may include a processor 151 and a memory 152.
- the memory 152 stores a computer program. When the computer program is executed by the processor 151, the processor 151 can execute the operation as described in any of the above embodiments. Add sample and mix method.
- the control unit 15 can drive the movement of the transfer unit 14 through the transfer unit drive unit; the transfer unit drive unit can be an XYZ three-axis motion platform, an air cylinder, a motor, and the like.
- sample adding and mixing method can be made into a control chip or a memory chip and then applied to various types of analysis and detection/analysis instruments, thereby expanding its application scope and application scenarios, and also improving The convenience of its application.
- the sample adding and mixing device may further include: a sample placing part 16 for placing the object to be added.
- the present application also provides a non-transitory computer-readable storage medium that stores a computer program 21.
- the processor executes the sample mixing and mixing as described in any of the above-mentioned embodiments. Even method.
- Non-volatile memory may include read only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory.
- Volatile memory may include random access memory (RAM) or external cache memory.
- RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous chain Channel (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.
- the application also provides a sample analysis device, which includes the sample adding and mixing device of any one of the above embodiments.
- the sample analysis device may be a coagulation analyzer, which is used to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation.
- the sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
A sample adding and mixing method, a sample adding and mixing apparatus, and a non-transitory computer readable storage medium. The sample adding and mixing method comprises the following steps: placing a sample setting part (2) at a suspended position (3); adding substances to be added into the sample setting part (2) by a sample adding part (5); and before the sample setting part (2) reaches a test position (4), performing a mixing operation on the sample setting part (2) by means of a transfer part (6), so that the substances added into the sample setting part (2) are well mixed to obtain a substrate to be tested. The method can effectively shorten the time from a mixing step to a test step.
Description
本申请涉及医疗检验技术领域,具体涉及一种加样混匀方法及装置、非临时性计算机可读存储介质。This application relates to the technical field of medical inspection, and specifically relates to a sample adding and mixing method and device, and a non-temporary computer-readable storage medium.
在医疗检验技术领域,将样本和试剂加入用于进行反应、检测、处理和/或分析的置样部中,并进行混匀,是一种常见的应用场景。其中,混匀操作是上述流程的关键环节。In the field of medical inspection technology, it is a common application scenario to add samples and reagents to the sample setting part for reaction, detection, processing and/or analysis, and to mix them. Among them, the mixing operation is a key link in the above process.
【发明内容】[Summary of the invention]
本申请针对以上问题的提出,而研制一种可以有效缩短混匀环节至检测环节的时间的加样混匀方法,同时还提供了一种能够实现上述方法的加样混匀装置、非临时性计算机可读存储介质。In response to the above problems, this application develops a sample adding and mixing method that can effectively shorten the time from the mixing link to the detection link. At the same time, it also provides a sample adding and mixing device that can realize the above method, which is non-temporary. Computer readable storage medium.
本申请采用的一个技术手段是:提供一种加样混匀方法,包括如下步骤:将置样部置于悬空位置上;加样部向所述置样部内添加待加物;在所述置样部达到检测位置之前,通过移载部对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到待测物。One of the technical means adopted in this application is to provide a sample adding and mixing method, which includes the following steps: placing the sample placing part on a suspended position; adding the sample adding part to the sample placing part; Before the sample portion reaches the detection position, the sample placement portion is mixed by the transfer portion, so that the additives in the sample placement portion are mixed uniformly to obtain the test object.
本申请采用的另一个技术手段是:提供一种加样混匀装置,包括:加样部及移栽部,其中,所述加样部用于在所述置样部至于悬空位置上时,向置样部内添加待加物;所述移载部用于将所述置样部由放置位置移动至所述悬空位置,并对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到所述待测物,并将所述置样部移至检测位置以对所述待测物进行检测;其中,所述放置位置与所述检测位置之间的距离大于所述悬空位置与所述检测位置之间的距离。Another technical means adopted in this application is to provide a sample adding and mixing device, comprising: a sample adding part and a transplanting part, wherein the sample adding part is used when the sample placing part is in a suspended position, Add objects to be added to the sample setting part; the transfer part is used to move the sample setting part from the placing position to the suspended position, and perform a mixing operation on the sample setting part to make the sample setting part The additives in the part are mixed to obtain the test object, and the sample placement part is moved to the detection position to detect the test object; wherein, the distance between the placement position and the detection position Greater than the distance between the suspended position and the detection position.
本申请采用的另一个技术手段是:提供一种非临时性计算机可读存储介质, 其中,所述非临时性计算机可读存储介质存储有计算机程序,所述计算机程序被处理器执行时,使得所述处理器执行如下步骤:通过移栽部将置样部置于悬空位置上;加样部向所述置样部内添加待加物;在所述置样部达到检测位置之前,通过移载部对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到待测物。Another technical means adopted in this application is to provide a non-transitory computer-readable storage medium, wherein the non-transitory computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, The processor executes the following steps: placing the sample placing part in a suspended position through the transplanting part; adding the sample adding part to the sample placing part; before the sample placing part reaches the detection position, by transferring The part performs a mixing operation on the sample setting part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.
在上述技术方案中,首先将置样部置于悬空位置上,然后向置样部添加待加物,并在置样部达到检测位置之前,通过移载部对置样部进行混匀操作,以使得置样部内的添加物混匀。从而无需等置样部到达检测位置之后执行混匀操作,从而缩短混匀环节至检测环节的时间。In the above technical solution, the sample setting part is first placed in a suspended position, and then the object to be added is added to the sample setting part, and before the sample setting part reaches the detection position, the sample setting part is mixed by the transfer part. So that the additives in the sample setting part are mixed evenly. Therefore, there is no need to wait for the sample setting part to perform the mixing operation after reaching the detection position, thereby shortening the time from the mixing step to the detection step.
为了更清楚地说明本申请实施例或现有技术中的技术方案,下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅是本申请的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only These are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.
其中:among them:
图1是一个实施例中加样混匀方法的流程图;Figure 1 is a flow chart of a sample adding and mixing method in an embodiment;
图2是一个实施例中加样混匀方法的流程图;Figure 2 is a flow chart of a sample adding and mixing method in an embodiment;
图3是一个实施例中加样混匀方法的流程图;Figure 3 is a flow chart of a sample adding and mixing method in an embodiment;
图4是一个实施例中加样部向置样部内加样的步骤流程图;Fig. 4 is a flow chart of the steps of the sample adding part adding sample to the sample placing part in an embodiment;
图5是一个实施例中加样混匀方法的部分流程图;Figure 5 is a partial flow chart of a sample adding and mixing method in an embodiment;
图6A、图6B、图6C、图6D是加样混匀方法的实施示例图;Fig. 6A, Fig. 6B, Fig. 6C, Fig. 6D are diagrams of implementation examples of the sample adding and mixing method;
图7是一个实施例中加样混匀方法的部分流程图;Figure 7 is a partial flow chart of a sample adding and mixing method in an embodiment;
图8是将置样部移动至检测位置的实施示例图;Figure 8 is a diagram of an implementation example of moving the sample setting part to the detection position;
图9是一个实施例中加样混匀方法的部分流程图;Figure 9 is a partial flow chart of a sample adding and mixing method in an embodiment;
图10是将置样部由放置位置移动至悬空位置的实施示例图;Figure 10 is a diagram showing an example of an implementation of moving the sample setting part from the placement position to the suspended position;
图11是一个实施例中加样混匀装置的结构框图;Figure 11 is a structural block diagram of a sample adding and mixing device in an embodiment;
图12是一个实施例中加样混匀装置的结构框图;Figure 12 is a structural block diagram of a sample adding and mixing device in an embodiment;
图13是一个实施例中非临时性计算机可读存储介质的结构框图。Fig. 13 is a structural block diagram of a non-transitory computer-readable storage medium in an embodiment.
为了使本申请的发明目的、技术方案及其技术效果更加清晰,以下结合附图和具体实施方式,对本申请进一步详细说明。应当理解的是,本说明书中描述的具体实施方式仅仅是为了解释本申请,并非为了限定本申请。在不冲突的情况下,本申请中的实施例及实施例中的特征可以相互组合。In order to make the purpose of the invention, technical solutions and technical effects of the present application clearer, the following further describes the present application in detail with reference to the accompanying drawings and specific implementations. It should be understood that the specific implementations described in this specification are only for explaining the application, not for limiting the application. In the case of no conflict, the embodiments in the application and the features in the embodiments can be combined with each other.
本申请提供了一种加样混匀方法,如图1所示,在一个实施例中,加样方法可以包括如下步骤:This application provides a sample adding and mixing method, as shown in FIG. 1. In one embodiment, the sample adding method may include the following steps:
步骤S12:将置样部置于悬空位置上。Step S12: Place the sample setting part in a suspended position.
其中,在置样部位于悬空位置的情况下,置样部处于悬空状态。Wherein, when the sample setting part is in a suspended position, the sample setting part is in a suspended state.
置样部是指可以置放样本或试剂的容器,其可以是样本容器、试剂容器,如反应杯、试管等。置样部的具体种类和形态对于本申请所要求保护的加样混匀方法不产生影响。置样部处于悬空状态是指置样部底部未置于在任何承载面上,位于悬空位置上的置样部可以由移载部来夹持或固定,以使得置样部保持悬空状态。移载部可以是机械手、手指气缸、夹爪等具有固定功能或夹持功能的部件或结构;在一个实施例中,悬空位置可以位于放置位置上方、检测位置上方、或者放置位置与检测位置之间。放置位置可以是在置于悬空位置之前,置样部所放置的位置。比如,在样本分析中,该放置位置可以是用于放置空的置样部的位置,也可以作为预加样位,即在放置位置上首先向置样部内添加一种或部分样本或试剂,在预加样位上加完样本或试剂的置样部在移至悬空位置之前,也可以先进行孵育。悬空位置可以是一个便于加样部到达也便于移动置样部至检测位置的位置,比如悬空位置的选取可以根据悬空位置与检测位置的距离、悬空位置与加样部初始位置的距离、悬空位置与置样部初始位置的距离 来确定,具体地,可以是上述三个距离之和最短的情况所对应的悬空位置。检测位置可以是能够放置置样部并对置样部内的待测物进行检测的位置,待测物可以为下述步骤S14中加样部添加的待加物,也可以是在下述步骤S14之前置样部内容置的样本、试剂,还可以是待加物与置样部内的其他样本、试剂进行反应获得的物质。The sample placement part refers to a container in which samples or reagents can be placed, and it can be a sample container, a reagent container, such as a reaction cup, a test tube, and the like. The specific type and form of the sample setting part have no influence on the sample adding and mixing method claimed in this application. The suspended state of the sample placing part means that the bottom of the sample placing part is not placed on any bearing surface, and the sample placing part in the suspended position can be clamped or fixed by the transfer part to keep the sample placing part suspended. The transfer part may be a part or structure with a fixing function or a clamping function such as a manipulator, a finger cylinder, a gripper, etc.; in one embodiment, the suspended position may be located above the placement position, above the detection position, or between the placement position and the detection position. between. The placement position may be the position where the sample placement part is placed before being placed in the suspended position. For example, in sample analysis, the placement position can be a position for placing an empty sample placement part, or it can be used as a pre-sampling position, that is, first add one or part of a sample or reagent to the sample placement part at the placement position. The sample placement part where the sample or reagent has been added to the pre-loading position can also be incubated before moving to the suspended position. The suspended position can be a position that is convenient for the sample application part to reach and move the sample setting part to the detection position. For example, the selection of the suspension position can be based on the distance between the suspended position and the detection position, the distance between the suspended position and the initial position of the sampling part, and the suspended position The distance from the initial position of the sample setting part is determined, specifically, it may be the suspended position corresponding to the case where the sum of the above three distances is the shortest. The detection position can be a position where the sample setting part can be placed and the test object in the sample setting part can be detected. The test object can be the object to be added by the sample adding part in the following step S14, or it can be in the following step S14 The samples and reagents placed in the pre-sample section may also be substances obtained by reacting the object to be added with other samples and reagents in the pre-sample section.
步骤S14:加样部向置样部内添加待加物。Step S14: the sample adding part adds the object to be added into the sample placing part.
加样部可以是样本针,也可以是能够吸取样本或试剂的其他部件。在步骤S14中,加样部向置样部添加的待加物可以只是样本,也可以只是试剂,或者是样本和试剂均添加,若只添加的是样本,则试剂需要在执行下述步骤S16之时或之前添加,若只添加的是试剂,则样本需要在执行步骤S14之前添加,比如,可以在置样部的放置区将样本添加至置样部,然后再将置样部移动至悬空位置。当然,置样部移动在前、加样部加样在后的方式,相比于加样部加样在前、置样部移动在后的方式,减少了已加样的置样部的移动时间,有利于降低样本污染的出现。当加样混匀方法应用于样本分析时,样本可以为血液关联样本,比如血液、血液成分等,血液成分可以为血浆、血细胞等;试剂可以为纤溶、抗纤溶、凝血、抗凝血等检测试剂。并且,加样部可以向处于移动状态的置样部添加待加物,也可以向处于悬空状态的置样部添加待加物,即当加样部向置样部添加待加物时,两者可以处于相对运动或相对静止的状态。The sample adding part can be a sample needle, or other parts capable of aspirating samples or reagents. In step S14, the substance to be added by the sample adding part to the sample placing part can be only the sample, or only the reagent, or both the sample and the reagent are added. If only the sample is added, the reagent needs to perform the following step S16 Add at the time or before. If only the reagent is added, the sample needs to be added before step S14. For example, the sample can be added to the sample setting part in the setting area of the sample setting part, and then the sample setting part can be moved to the suspension position. Of course, the way that the sample setting part moves in the front and the sample application part is loaded after is compared to the method where the sample setting part is moved before and the sample setting part moves behind, which reduces the movement of the sample setting part that has already been loaded. Time helps reduce the occurrence of sample contamination. When the sample addition and mixing method is applied to sample analysis, the sample can be blood-related samples, such as blood, blood components, etc., blood components can be plasma, blood cells, etc.; reagents can be fibrinolysis, antifibrinolysis, coagulation, and anticoagulation And other detection reagents. In addition, the sample adding section can add objects to be added to the sample placing section in a moving state, or add objects to be added to the sample placing section in a suspended state, that is, when the sample adding section adds objects to be added to the sample placing section, the two People can be in relative motion or relatively static state.
步骤S16:在置样部达到检测位置之前,通过移载部对置样部进行混匀操作,以使得置样部内的添加物混匀。Step S16: Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly.
其中,在移载部的作用下,置样部具有移动状态和混匀状态中的至少一种状态。Wherein, under the action of the transfer part, the sample setting part has at least one of a moving state and a mixing state.
如前所述,移载部可以是机械手、手指气缸、夹爪等具有夹持功能的部件或结构。其既可以驱动置样部使其处于移动状态,比如将置样部移送至悬空位置、移送至检测位置,也可以驱动置样部使其处于混匀状态,进而对置样部内的待加物进行摇匀,例如,样本和/或试剂发生混匀,例如可以将血液关联样本 与检测试剂进行摇匀。当然,在移载部的作用下,置样部可以既处于移动状态同时也处于混匀状态,比如一边移动一边进行混匀。在移载部的作用下,置样部还可以在预定时间内持续保持在悬空位置上,预定时间可以为实施步骤S14或步骤S16所需的时间。As mentioned above, the transfer part can be a part or structure with a clamping function, such as a manipulator, a finger cylinder, or a clamping jaw. It can drive the sample setting part to move, for example, transfer the sample setting part to the suspended position, transfer to the detection position, or drive the sample setting part to be in a state of mixing, and then face the objects to be added in the sample setting part. Shaking is performed, for example, the sample and/or reagent are mixed, for example, the blood-related sample and the detection reagent can be shaken. Of course, under the action of the transfer part, the sample placement part can be in both a moving state and a mixing state, for example, mixing while moving. Under the action of the transfer part, the sample setting part can also be kept in the suspended position for a predetermined time, and the predetermined time can be the time required to implement step S14 or step S16.
在一个实施例中,置样部处于混匀状态的位置可以为悬空位置、或者在置样部由悬空位置移动至检测位置过程中能够经过的任一位置。置样部具体进行混匀的位置可以为悬空位置,在这种情况下,加样部也直接向位于悬空位置上的置样部进行待加物添加,并在加样后直接在悬空位置对置样部进行混匀操作,以使得置样部内的添加物混匀。对置样部具体进行混匀操作的位置可以为在置样部由悬空位置移动至检测位置过程中能够经过的任一位置,即可以在移载部将置样部由悬空位置移送至检测位置的过程中对置样部进行混匀操作,悬空位置至检测位置的移动路径可以有多条,只要是能够将置样部由悬空位置移动至检测位置即可。并且,置样部在移动过程中可以持续保持移动状态,当然也可以移动一段路径后,暂停移动,暂停一定时间后,再继续进入移动状态。移动路径和置样部在移动过程中的所处状态可以根据需求设置。比如置样部由悬空位置可以经过移动路径一到达检测位置,也可以经过移动路径二到达检测位置,还可以经过其他移动路径到达检测位置,任一移动路径上的任一点位置均可以是对置样部进行混匀操作的位置,当然,该位置可以是能够容置置样部的检测位置的上方。混匀操作的实施位置可以根据本次待加物的最佳检测时间来确定。In an embodiment, the position where the sample setting part is in the mixing state may be a suspended position, or any position that the sample setting part can pass during the process of moving from the suspended position to the detection position. The specific mixing position of the sample setting part can be the suspended position. In this case, the sample addition part also directly adds the substance to be added to the sample setting part located in the suspended position, and directly adjusts the sample in the suspended position after the sample is added. The sample setting part performs a mixing operation to mix the additives in the sample setting part evenly. The position where the sample setting part is specifically mixed can be any position that can be passed during the process of moving the sample setting part from the suspended position to the detection position, that is, the sample setting part can be transferred from the suspended position to the detection position in the transfer part During the process of mixing the sample setting part, there can be multiple moving paths from the suspended position to the detection position, as long as the sample setting part can be moved from the suspended position to the detection position. In addition, the sample setting part can keep moving during the moving process, of course, it can also pause after moving a certain path, and then continue to enter the moving state after pausing for a certain period of time. The moving path and the state of the sample setting part during the moving process can be set according to requirements. For example, the sample setting part can reach the detection position through moving path 1 from the suspended position, or reach the detection position through moving path 2, or reach the detection position through other moving paths. Any point on any moving path can be opposite. The position where the sample part performs the mixing operation, of course, this position may be above the detection position where the sample part can be accommodated. The implementation position of the mixing operation can be determined according to the best detection time of the object to be added this time.
在一个实施例中,通过移载部对置样部进行混匀操作时,可通过移载部对其夹持的置样部施加能够使得置样部执行混匀动作的驱动力。In one embodiment, when the sample setting part is mixed by the transfer part, the sample setting part clamped by the transfer part can be used to apply a driving force that enables the sample setting part to perform the mixing action.
驱动力包括转动力、摆动力和振动力中的至少一种,进而置样部实现转动混匀、摆动混匀、振动混匀、或者其他混匀动作。当然,移载部施加的转动力、摆动力和振动力可以叠加出现或依次出现,比如置样部时而执行转动混匀、时而执行振动混匀等。同时,混匀动作的持续时间也可以根据实际情况进行调整。The driving force includes at least one of a rotation force, a swing force and a vibration force, and the sample setting part realizes rotation mixing, swing mixing, vibration mixing, or other mixing actions. Of course, the rotational force, swing force, and vibration force applied by the transfer part can appear superimposed or appear in sequence, for example, the sample placement part sometimes performs rotation mixing and sometimes vibration mixing. At the same time, the duration of the mixing action can also be adjusted according to the actual situation.
需要指出的是,相关技术中,可以通过以下两种方式对样本进行混匀。It should be pointed out that in related technologies, samples can be mixed in the following two ways.
一种是混匀机构设置在置样部的放置区,完成样本和试剂的加样操作后,直接在放置位置上进行混匀,然后再到检测位置进行样本的检测操作。由于混匀后需要将置样部经过从放置区至检测位置的运动,所需时间较长,容易错过样本的最佳检测时间,尤其是对于反应较快的样本检测过程。这种混匀方式对检验速度和性能均有不利影响。One is that the mixing mechanism is set in the placement area of the sample placement part. After the sample and reagent loading operations are completed, they are directly mixed at the placement location, and then the sample is detected at the detection location. Since the sample placement part needs to move from the placement area to the detection position after mixing, it takes a long time and it is easy to miss the optimal detection time of the sample, especially for the quick response sample detection process. This mixing method has an adverse effect on the inspection speed and performance.
另一种是混匀机构设置在样本的检测位置,完成样本和试剂的加样操作后移送至检测位置,并在检测位置上进行混匀。这种混匀方式结构相对复杂,并且对于多个样本的统一检测和分析,则需要在每个检测通道上均增加混匀机构,成本明显增高。本实施例中的加样混匀方法中,首先将置样部置于悬空位置上,然后向置样部添加待加物,之后通过移载部对置样部进行混匀。对置样部进行混匀的位置可以为置样部处于混匀状态的位置,如悬空位置、或者悬空位置移动至检测位置过程中能够经过的任一位置,进而可以实现加样混匀方法无需在置样部放置区或检测位置额外设置混匀机构,能够有效降低成本;同时,对位于悬空位置的置样部进行加样后的混匀和移动,便于减少置样部移动至检测位置的时间,也避免了混匀后最佳检测时间的错过问题,有利于提高检测效率和检测结果的准确性。当应用于样本分析设备时,比如应用在化学发光分析仪、凝血分析仪等临床检验设备中时,能够实现在控制成本的基础上,有效避免最佳样本分析及检测时间的错过问题,利于获得更加准确的样本分析结果。在置样部处于混匀状态的位置为悬空位置、或者悬空位置与检测位置之间位置的情况下,由于对置样部进行悬空混匀,因此,还可以有效的避免置样部与其承载面发生碰撞的问题。The other is that the mixing mechanism is set at the detection position of the sample, and the sample and reagent are transferred to the detection position after the sample and reagent addition operation is completed, and the mixing is performed at the detection position. The structure of this mixing method is relatively complicated, and for the unified detection and analysis of multiple samples, a mixing mechanism needs to be added to each detection channel, which significantly increases the cost. In the sample adding and mixing method in this embodiment, the sample placing part is first placed in a suspended position, and then the object to be added is added to the sample placing part, and then the sample placing part is mixed by the transfer part. The position where the sample setting part is mixed can be the position where the sample setting part is in the mixing state, such as a suspended position, or any position that the suspended position can pass during the process of moving to the detection position, so that the sample addition and mixing method can be realized. An additional mixing mechanism is provided in the placement area or detection position of the sample placement part, which can effectively reduce costs; at the same time, the sample placement part located in the suspended position is mixed and moved after sample addition, which is convenient to reduce the movement of the sample placement part to the detection position. Time, also avoids the problem of missing the best detection time after mixing, which is beneficial to improve the detection efficiency and the accuracy of the detection results. When applied to sample analysis equipment, such as chemiluminescence analyzers, coagulation analyzers and other clinical testing equipment, it can effectively avoid the problem of missing the best sample analysis and detection time on the basis of cost control, which is conducive to obtaining More accurate sample analysis results. In the case where the position where the sample setting part is in the mixing state is a suspended position, or a position between the suspended position and the detection position, because the sample setting part is suspended and mixed, it can also effectively avoid the sample setting part and its bearing surface. The problem of collision.
在一个实施例中,如图2和图3所示,在加样部向置样部内添加待加物的步骤之后,加样混匀方法还可以包括如下步骤:In one embodiment, as shown in Figures 2 and 3, after the step of adding the object to be added to the sample placing part by the sample adding part, the sample adding and mixing method may further include the following steps:
步骤S20:移载部将置样部移至检测位置。Step S20: The transfer part moves the sample setting part to the detection position.
具体地,如图2所示,步骤S20可以在步骤S16之后执行,即移送至检测位置上的置样部已完成加入待加物和对待加物进行混匀的操作,则直接将置样 部移送至检测位置上进行样本检测。具体地,如图3所示,步骤S20也可以在步骤S14和步骤S16之间进行,即移送至检测位置上的置样部已经过待加物的加样但未进行混匀操作,移送至检测位置后首先通过移载部对置样部进行混匀,然后再进行样本检测。两种方式可以根据实际的样本反应和分析要求进行选用。Specifically, as shown in FIG. 2, step S20 can be performed after step S16, that is, the sample setting part transferred to the detection position has completed the operations of adding the object to be added and mixing the object to be added, then the sample setting part is directly Transfer to the detection position for sample detection. Specifically, as shown in FIG. 3, step S20 can also be performed between step S14 and step S16, that is, the sample placement part that has been transferred to the detection position has passed the sample addition of the object to be added but has not performed the mixing operation, and is transferred to After detecting the position, firstly mix the sample placement part through the transfer part, and then perform the sample detection. The two methods can be selected according to the actual sample reaction and analysis requirements.
在一个实施例中,请参阅图4,加样混匀方法还可以包括如下步骤:In one embodiment, referring to FIG. 4, the method for adding and mixing samples may further include the following steps:
步骤S32:检测置样部是否置于移载部上。Step S32: Detect whether the sample setting part is placed on the transfer part.
具体地,可以通过传感器、感应件、检测部件等检测移载部上是否夹持有置样部。Specifically, a sensor, a sensing element, a detection component, etc. can be used to detect whether the sample placement part is clamped on the transfer part.
步骤S34:在置样部置于移载部上时,获取置样部的当前位置。Step S34: When the sample setting part is placed on the transfer part, the current position of the sample setting part is acquired.
比如获知置样部当前位于悬空位置、检测位置等,具体地,可以通过检测夹持有置样部的移载部的具***置,进而来获取置样部的当前位置。在置样部未置于移载部上的情况下,可以控制移载部进行置样部的夹取操作。For example, it is known that the sample setting part is currently located in the suspended position, the detection position, etc. Specifically, the current position of the sample setting part can be obtained by detecting the specific position of the transfer part holding the sample setting part. When the sample setting part is not placed on the transfer part, the transfer part can be controlled to perform the clamping operation of the sample setting part.
步骤S36:根据置样部的当前位置,通过移载部来控制置样部的所处状态。Step S36: According to the current position of the sample setting part, the state of the sample setting part is controlled by the transfer part.
通过上述检测和获取结果,便于控制和调整置样部的所处状态,比如控制置样部处于悬空状态、移动状态、混匀状态等。例如,当移载部上夹持有置样部但未到达悬空位置时,则可以通过移载部控制置样部处于移动状态。当移载部上夹持有置样部且到达悬空位置时,则可以控制置样部处于悬空状态,同时可以控制加样部进行加样工作,预定时间后,则可以控制置样部进入混匀状态或移动状态等。Through the above detection and acquisition results, it is convenient to control and adjust the state of the sample setting part, such as controlling the sample setting part to be in a suspended state, a moving state, a mixing state, and so on. For example, when the sample setting part is clamped on the transfer part but has not reached the suspended position, the transfer part can be used to control the sample setting part to be in a moving state. When the sample setting part is clamped on the transfer part and reaches the suspended position, the sample setting part can be controlled to be in a suspended state, and at the same time, the sample adding part can be controlled to perform the sample adding work. After a predetermined time, the sample setting part can be controlled to enter the mixed position. Uniform state or mobile state, etc.
在一个实施例中,如图5所示,加样部向置样部添加待加物的步骤S14可以包括:In an embodiment, as shown in FIG. 5, the step S14 of adding the object to be added by the sample adding part to the sample placing part may include:
步骤S142:加样部与置样部彼此靠近。Step S142: the sample adding part and the sample setting part are close to each other.
加样前的置样部可以是空的置样部,即加样前,置样部内无样本或试剂等液体,加样前的置样部也可以事先内置有液体,即向已有液体的置样部中添加样本或试剂。加样前,可以控制加样部向置样部所在位置进行移动,置样部所在位置可以为悬空位置;也可以控制置样部向加样部移动,还可以控制加样部 与置样部均进行移动而向彼此靠近。The sample setting part before sample addition can be an empty sample setting part, that is, before sample addition, there is no liquid such as sample or reagent in the sample setting part, and the sample setting part before sample addition can also be filled with liquid in advance, that is, to the existing liquid Add samples or reagents to the sample setting section. Before adding samples, you can control the sample adding part to move to the position of the sample setting part, the position of the sample setting part can be a suspended position; you can also control the sample setting part to move to the sample part, and you can also control the sample adding part and the sample setting part. Both move towards each other.
步骤S144:加样部在加样位置上将待加物添加至置样部。Step S144: the sample adding part adds the object to be added to the sample placing part at the sample adding position.
加样位置是指加样部进行待加物添加时所处的位置,该加样位置可以事先设定。The sample adding position refers to the position where the sample adding part is when adding the object to be added, and the sample adding position can be set in advance.
待加物可以是样本、试剂等,若加样部为样本针,则待加物可以由样本针的排样口排出。The objects to be added can be samples, reagents, etc., if the sample adding part is a sample needle, the objects to be added can be discharged from the sample ejection port of the sample needle.
步骤S146:在加样完成后,加样部与置样部彼此远离。Step S146: After the sample application is completed, the sample application part and the sample setting part are far away from each other.
加样完成后,可以控制加样部移动离开置样部,也可以控制置样部移动离开加样部,还可以控制加样部和置样部均朝远离对方的方向移动。After the sample is added, you can control the sample adding part to move away from the sample placing part, you can also control the sample placing part to move away from the sample adding part, and you can also control both the sample adding part and the sample placing part to move away from each other.
如图6B、图6C所示,加样部5移动至置于悬空位置3上的置样部2内进行加样。As shown in FIG. 6B and FIG. 6C, the sample adding part 5 moves to the sample placing part 2 placed on the suspended position 3 to add samples.
在一个实施例中,可以在排出待加物后,处于加样位置的加样部5与置样部2内的液面之间满足第一状态或第二状态。其中,第一状态为加样部5的触液位置恰好接触置样部2内的液面,即二者处于同一高度水平上;第二状态为加样部5的触液位置位于置样部2内的液面上方一定距离的位置处,且与置样部2内的液面的距离小于预设距离。如图6B所示,加样部5在排出待加物后,加样部5的触液位置位于置样部2内的液面7上方,且与置样部2内的液面7的距离小于预设距离,加样部5与置样部2内的液面7之间处于第二状态;如图6C所示,加样部5在排出待加物后,加样部5的触液位置恰好接触置样部2内的液面7,即加样部5与置样部2内的液面7之间处于第一状态。In one embodiment, after the objects to be added are discharged, the first state or the second state may be satisfied between the sample adding portion 5 at the sample adding position and the liquid level in the sample placing portion 2. Among them, the first state is that the liquid contact position of the sample adding section 5 just touches the liquid surface in the sample placing section 2, that is, the two are at the same height level; the second state is that the liquid contact position of the sample adding section 5 is located at the sample placing section At a certain distance above the liquid surface in 2 and the distance from the liquid surface in the sample placement part 2 is less than a preset distance. As shown in Figure 6B, after the sample adding part 5 discharges the objects to be added, the liquid contact position of the sample adding part 5 is located above the liquid surface 7 in the sample placing part 2 and the distance from the liquid surface 7 in the sample placing part 2 If the distance is less than the preset distance, the liquid level 7 in the sample adding portion 5 and the sample placing portion 2 is in the second state; as shown in Fig. 6C, the sample adding portion 5 touches the liquid after the object to be added is discharged. The position just touches the liquid surface 7 in the sample setting part 2, that is, the liquid surface 7 in the sample setting part 2 is in the first state between the sample application part 5 and the liquid surface 7 in the sample setting part 2.
加样部的触液位置可以为加样部的前端部与加样部的排样口之间的任意位置,前端部靠近排样口,具体地,可以是前端部、排样口、前端部与排样口之间的中间位置等。The liquid contact position of the sample adding part can be any position between the front end of the sample adding part and the discharge port of the sample adding part, and the front end is close to the discharge port. Specifically, it can be the front end, the discharge port, or the front end. And the middle position between the sample port and so on.
预设距离可以为加样部进行样本排出操作时,形成的一个自然下落的样本液滴高度,预设距离可以通过事先的实验获知,比如采用该加样部进行滴样操作,并测量获知形成的一个自然下落的样本液滴高度值,即可作为预设距离的 数值,实际应用时,预设距离可以取值0~1mm,例如,0mm、0.5mm、1mm。无论第一状态还是第二状态,均可以使得加样部上形成的样本液滴与置样部内的液面接触,进而实现样本液滴与置样部内的液面之间产生粘滞力,同时置样部内的液体表面张力也会对样本液滴产生作用。进而随着加样部的向上移动,在置样部内的液体粘滞力和表面张力、以及样本液滴自身重力的共同作用下落入置样部内,避免了样本挂壁和残留情况的发生,提高加样的准确度,从而在应用于样本分析时,能够提高样本分析结果的准确性。The preset distance can be the height of a naturally falling sample drop formed when the sample dispensing part performs the sample discharge operation. The preset distance can be obtained through prior experiments. For example, the sample dispensing part is used to perform the sample drop operation, and the formation is obtained by measurement. The height value of a sample droplet that falls naturally can be used as the value of the preset distance. In practical applications, the preset distance can take a value of 0 to 1 mm, for example, 0 mm, 0.5 mm, and 1 mm. Regardless of the first state or the second state, the sample droplet formed on the sample loading part can be brought into contact with the liquid surface in the sample loading part, thereby achieving viscous force between the sample droplet and the liquid surface in the sample loading part, and at the same time The surface tension of the liquid in the sample placement part will also have an effect on the sample droplets. Furthermore, as the sample adding part moves upward, the liquid viscosity and surface tension in the sample placing part, as well as the gravity of the sample droplet itself, fall into the sample placing part, which avoids the occurrence of sample hanging and residue, and improves The accuracy of sample addition can improve the accuracy of sample analysis results when applied to sample analysis.
在一个实施例中,在加样部向置样部靠近的步骤S32之前,加样混匀方法还可以包括如下步骤:将加样部移动至置样部的上方。In an embodiment, before the step S32 in which the sample adding part approaches the sample placing part, the sample adding and mixing method may further include the following step: moving the sample adding part above the sample placing part.
加样部吸取完样本后,与置样部可以形成竖直方向上的上方和下方的位置关系,具体地,可以加样部移动至置样部上方,当然,也可以在执行将置样部置于悬空位置的操作时,直接将置样部移动到加样部下方,那么则可以省略该步骤的执行。After the sample application part has absorbed the sample, it can form an upper and lower positional relationship with the sample setting part in the vertical direction. Specifically, the sample application part can be moved above the sample setting part. Of course, the sample setting part can also be When the operation is placed in the suspended position, directly move the sample setting part below the sample application part, then the execution of this step can be omitted.
在一个实施例中,加样部向置样部靠近的步骤与将置样部置于悬空位置上的步骤可以同时执行。In an embodiment, the step of approaching the sample application part to the sample placement part and the step of placing the sample placement part in a suspended position can be performed at the same time.
若加样部的初始位置的设定使得其也需要向悬空位置附近移动,为了节省部件移载时间,可以在执行将置样部置于悬空位置操作的同时,同步控制加样部移动,使得其移动至置于悬空位置上的置样部上方。If the initial position of the sample loading part is set so that it also needs to move to the vicinity of the suspended position, in order to save the time of part transfer, you can simultaneously control the movement of the sample loading part while performing the operation of placing the sample loading part in the suspended position, so that It moves to above the sample placement part placed in the suspended position.
在一个实施例中,将置样部置于悬空位置上的步骤可以为:移载部将置样部由放置位置移动至悬空位置。其中,放置位置与检测位置之间的距离大于悬空位置与检测位置之间的距离,进而由悬空位置移动至检测位置可以节省移载部的移动路径,减少移载部的运动时间。In one embodiment, the step of placing the sample placement part in the suspended position may be: the transfer part moves the sample placement part from the placement position to the suspended position. Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position, and moving from the suspended position to the detection position can save the moving path of the transfer part and reduce the movement time of the transfer part.
放置位置可以为置样部的放置区,例如,多个置样部均置于位于放置区上的置样部盒内,或者置样部放置在放置区上,放置区为置样部提供承载置样部底部的支承面。在相关技术中的加样方法中,置样部通常在放置区进行加样的操作,完成混匀后,再将置样部移动到检测位置,从而需要耗费较多的时间。 如图6A所示,置样部2可以先由放置位置1移动至悬空位置3,然后再由悬空位置3移动至检测位置4。可以通过移载部进行置样部的放置和移动操作。移载部夹持和抓取置样部,并带动置样部进行移动,当到达相应位置后,若该位置上未有支承面,如前述的悬空位置,则移载部把持固定置样部使其保持在相应位置上停留,若该位置上有支承面,如检测位置,则移载部释放置样部并将放置在相应位置上。The placement position can be the placement area of the sample placement section, for example, multiple placement sections are placed in the placement section box located on the placement area, or the placement section is placed on the placement area, and the placement area provides a bearing for the placement section. The supporting surface at the bottom of the sample placement section. In the sample adding method in the related art, the sample setting part usually performs the sample adding operation in the placing area, and after the mixing is completed, the sample setting part is moved to the detection position, which takes a lot of time. As shown in FIG. 6A, the sample setting part 2 can be moved from the placement position 1 to the suspended position 3 first, and then from the suspended position 3 to the detection position 4. The placing and moving operation of the sample setting part can be carried out through the transfer part. The transfer part clamps and grabs the sample setting part, and drives the sample setting part to move. After reaching the corresponding position, if there is no supporting surface at the position, such as the above-mentioned suspended position, the transfer part holds and fixes the sample setting part Keep it at the corresponding position. If there is a supporting surface at the position, such as the detection position, the transfer part releases the sample placement part and places it in the corresponding position.
如图6D所示,置于检测位置4上的置样部2此时已经完成了加样和混匀操作,则可以进行待测物如混匀后的样本和/或试剂的检测操作。As shown in FIG. 6D, the sample placement part 2 placed on the detection position 4 has completed the sample addition and mixing operations at this time, and the detection operations of the analyte, such as the mixed sample and/or reagent, can be performed.
在一个实施例中,请参阅图7,在置样部处于混匀状态的位置为悬空位置的情况下,移载部将置样部移至检测位置的步骤S20可以包括:In one embodiment, referring to FIG. 7, when the position where the sample setting part is in the mixing state is a suspended position, the step S20 of moving the sample setting part to the detection position by the transfer part may include:
S21:移载部将置样部从悬空位置移送至检测位置。S21: The transfer part transfers the sample placement part from the suspended position to the detection position.
S22:移载部释放置样部,将置样部置于检测位置上。S22: The transfer part releases the sample setting part and places the sample setting part at the detection position.
如图8所示,移载部6由把持固定置样部2使其位于悬空位置3上的状态切换到带动置样部2移动的状态,并由悬空位置3移动至检测位置4,并在到达检测位置4后释放置样部2,运动过程平稳顺畅。As shown in Figure 8, the transfer part 6 is switched from the state of holding and fixing the sample placing part 2 in the suspended position 3 to the state of driving the sample placing part 2 to move, and moves from the suspended position 3 to the detection position 4, and After reaching the detection position 4, the sample setting part 2 is released, and the movement process is smooth and smooth.
在一个实施例中,请参阅图9,移载部将置样部由放置位置移动至悬空位置的步骤可以包括:In one embodiment, referring to FIG. 9, the step of moving the sample placement part from the placement position to the suspended position by the transfer part may include:
步骤S41:移载部将位于放置位置上的置样部进行夹取;Step S41: the transfer part clamps the sample placement part located at the placement position;
步骤S42:移载部将置样部移送至悬空位置。Step S42: The transfer part transfers the sample setting part to the suspended position.
如图10所示,移载部6夹取置样部2,并由放置位置1移动至悬空位置3。As shown in FIG. 10, the transfer part 6 clamps the sample part 2 and moves from the placement position 1 to the suspended position 3.
本申请还提供了一种样本分析方法,其包括上述任一实施例的加样混匀方法。该样本分析方法可以应用于凝血分析仪中,用于对血液进行纤溶、抗纤溶、凝血、抗凝血等功能分析。样本可以为血液样本、血液检测试剂等,比如血液、血液成分,纤溶、抗纤溶、凝血、抗凝血等检测试剂,血液成分可以为血浆、血细胞等。The application also provides a sample analysis method, which includes the sample adding and mixing method of any one of the foregoing embodiments. The sample analysis method can be applied to a coagulation analyzer to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation. The sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.
本申请还提供了一种加样混匀装置,在一个实施例中,如图11所示,加样 混匀装置可以包括:加样部11、第一加样驱动部12、第二加样驱动部13、移载部14和控制部15。加样部11用于吸取待加物并向置样部内加样,具体可以为样本针;第一加样驱动部12用于驱动加样部11的运动;第一加样驱动12部可以是为XYZ三轴运动平台、转动式运动平台、电机等动力元件,当然,也可以是与置样部共用的移载部14;第二加样驱动部13用于驱动加样部11实现待加物的吸取或排出操作;第二加样驱动部13可以是注射器;注射器工作原理可以为注射器内柱塞在驱动电机的带动下进行上下运动,进而引起密封腔体的体积变化形成不同真空度,来达到液体的吸入和排出。第二加样驱动部13与加样部11相连接,可以用于带动加样部11实现样本或试剂的吸取或排出操作。移载部14具备用于夹持和释放置样部11的夹持机构和用于对置样部进行混匀的混匀机构;控制部15用于对第一加样驱动部12、第二加样驱动部13和移载部14进行控制,可包括处理器151和存储器152,存储器152存储有计算机程序,计算机程序被处理器151执行时,使得处理器151执行如上述任一实施例的加样混匀方法。控制部15可以通过移载部驱动部来驱动移载部14的运动;该移载部驱动部可以为XYZ三轴运动平台、气缸、电机等。The application also provides a sample adding and mixing device. In one embodiment, as shown in FIG. 11, the sample adding and mixing device may include: a sample adding portion 11, a first sample adding driving portion 12, and a second sample adding portion. The drive unit 13, the transfer unit 14 and the control unit 15. The sample adding part 11 is used for sucking the object to be added and adding the sample into the sample placing part, which can be a sample needle; the first sample adding driving part 12 is used for driving the movement of the sample adding part 11; the first sample adding driving 12 can be It is a power element such as an XYZ three-axis motion platform, a rotary motion platform, and a motor. Of course, it can also be the transfer part 14 shared with the sample setting part; the second sample adding driving part 13 is used to drive the sample adding part 11 to realize the adding The operation of sucking or discharging the substance; the second sample feeding driving part 13 can be a syringe; the working principle of the syringe can be that the plunger in the syringe moves up and down under the drive of the driving motor, which causes the volume of the sealed cavity to change to form different vacuum degrees. To achieve the suction and discharge of liquid. The second sample adding driving part 13 is connected to the sample adding part 11 and can be used to drive the sample adding part 11 to realize the suction or discharge operation of the sample or the reagent. The transfer part 14 is provided with a clamping mechanism for clamping and releasing the sample setting part 11 and a mixing mechanism for mixing the sample setting part; the control part 15 is used for controlling the first sample driving part 12 and the second The sample loading drive unit 13 and the transfer unit 14 are controlled, and may include a processor 151 and a memory 152. The memory 152 stores a computer program. When the computer program is executed by the processor 151, the processor 151 can execute the operation as described in any of the above embodiments. Add sample and mix method. The control unit 15 can drive the movement of the transfer unit 14 through the transfer unit drive unit; the transfer unit drive unit can be an XYZ three-axis motion platform, an air cylinder, a motor, and the like.
本领域技术人员可以理解,上述的加样混匀方法可以被制作成控制芯片或存储芯片后应用到各种类型的分析检测/分析仪器上,从而扩大其应用范围和应用场景,同时也提高了其应用的便捷性。Those skilled in the art can understand that the above-mentioned sample adding and mixing method can be made into a control chip or a memory chip and then applied to various types of analysis and detection/analysis instruments, thereby expanding its application scope and application scenarios, and also improving The convenience of its application.
在一个实施例中,请参阅图12,加样混匀装置还可以包括:置样部16,用于放置待加物。In one embodiment, referring to FIG. 12, the sample adding and mixing device may further include: a sample placing part 16 for placing the object to be added.
需要指出的是,上述加样混匀装置中的各部件能够互相配合以实现前述的加样混匀方法。It should be pointed out that the components of the above-mentioned sample adding and mixing device can cooperate with each other to realize the aforementioned sample adding and mixing method.
请参阅图13,本申请还提供了一种非临时性计算机可读存储介质,存储有计算机程序21,计算机程序21被处理器执行时,使得处理器执行如上述任一实施例的加样混匀方法。Referring to FIG. 13, the present application also provides a non-transitory computer-readable storage medium that stores a computer program 21. When the computer program 21 is executed by the processor, the processor executes the sample mixing and mixing as described in any of the above-mentioned embodiments. Even method.
本领域普通技术人员可以理解实现上述实施例方法中的全部或部分流程, 是可以通过计算机程序来指令相关的硬件来完成,该计算机程序21可存储于一非易失性计算机可读取存储介质中,该程序在执行时,可包括如上述各方法的实施例的流程。其中,本申请所提供的各实施例中所使用的对存储器、存储、数据库或其它介质的任何引用,均可包括非易失性和/或易失性存储器。非易失性存储器可包括只读存储器(ROM)、可编程ROM(PROM)、电可编程ROM(EPROM)、电可擦除可编程ROM(EEPROM)或闪存。易失性存储器可包括随机存取存储器(RAM)或者外部高速缓冲存储器。作为说明而非局限,RAM以多种形式可得,诸如静态RAM(SRAM)、动态RAM(DRAM)、同步DRAM(SDRAM)、双数据率SDRAM(DDRSDRAM)、增强型SDRAM(ESDRAM)、同步链路(Synchlink)DRAM(SLDRAM)、存储器总线(Rambus)直接RAM(RDRAM)、直接存储器总线动态RAM(DRDRAM)、以及存储器总线动态RAM(RDRAM)等。A person of ordinary skill in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be implemented by instructing relevant hardware through a computer program. The computer program 21 can be stored in a non-volatile computer readable storage medium. Here, when the program is executed, it may include the procedures of the above-mentioned method embodiments. Wherein, any reference to memory, storage, database, or other media used in the embodiments provided in this application may include non-volatile and/or volatile memory. Non-volatile memory may include read only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. As an illustration and not a limitation, RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous chain Channel (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.
本申请还提供了一种样本分析装置,其包括上述任一实施例的加样混匀装置。样本分析装置可以为凝血分析仪,用于对血液进行纤溶、抗纤溶、凝血、抗凝血等功能分析。样本可以为血液样本、血液检测试剂等,比如血液、血液成分,纤溶、抗纤溶、凝血、抗凝血等检测试剂,血液成分可以为血浆、血细胞等。The application also provides a sample analysis device, which includes the sample adding and mixing device of any one of the above embodiments. The sample analysis device may be a coagulation analyzer, which is used to perform functional analysis of blood such as fibrinolysis, antifibrinolysis, coagulation, and anticoagulation. The sample may be a blood sample, a blood test reagent, etc., such as blood, blood components, fibrinolysis, antifibrinolysis, coagulation, anticoagulation, and other test reagents, and the blood components may be plasma, blood cells, and the like.
以上,仅为本申请较佳的具体实施方式,但本申请的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本申请揭露的技术范围内,根据本申请的技术方案及其发明构思加以等同替换或改变,都应涵盖在本申请的保护范围之内。此外,尽管本说明书中使用了一些特定的术语,但这些术语只是为了方便说明,并不对本申请构成任何限制。The above are only preferred specific implementations of this application, but the protection scope of this application is not limited to this. Anyone familiar with the technical field within the technical scope disclosed in this application, according to the technical solution of this application and its The equivalent replacement or change of the inventive concept shall be covered by the protection scope of this application. In addition, although some specific terms are used in this specification, these terms are only for convenience of description and do not constitute any limitation to this application.
Claims (20)
- 一种加样混匀方法,包括:A method for adding and mixing samples, including:将置样部置于悬空位置上;Place the sample setting part in a suspended position;加样部向所述置样部内添加待加物;The sample adding part adds an object to be added into the sample placing part;在所述置样部达到检测位置之前,通过移载部对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到待测物。Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.
- 根据权利要求1所述的加样混匀方法,其中,所述置样部处于所述混匀状态的位置为所述悬空位置、或者在所述置样部由所述悬空位置移动至检测位置过程中能够经过的任一位置;The sample adding and mixing method according to claim 1, wherein the position where the sample setting part is in the mixing state is the suspended position, or the sample setting part moves from the suspended position to the detection position Any position that can be passed during the process;所述检测位置是放置所述置样部并对所述置样部内的所述待测物进行检测的位置。The detection position is a position where the sample setting part is placed and the test object in the sample setting part is detected.
- 根据权利要求1所述的加样混匀方法,其中,在所述加样部向所述置样部添加待加物的步骤之后,还包括:The sample adding and mixing method according to claim 1, wherein after the step of adding the sample to the sample placing portion by the sample adding portion, the method further comprises:所述移载部将所述置样部移至检测位置;The transfer part moves the sample placement part to a detection position;其中,所述检测位置是放置所述置样部并对所述置样部内的所述待测物进行检测的位置。Wherein, the detection position is a position where the sample setting part is placed and the test object in the sample setting part is detected.
- 根据权利要求3所述的加样混匀方法,其中,在所述置样部处于所述混匀状态的位置为所述悬空位置的情况下,所述移载部将所述置样部移至所述检测位置的步骤包括:The sample adding and mixing method according to claim 3, wherein when the position where the sample setting part is in the mixing state is the suspended position, the transfer part moves the sample setting part The steps to the detection position include:所述移载部将所述置样部从所述悬空位置移送至所述检测位置;The transfer part transfers the sample placement part from the suspended position to the detection position;所述移载部释放所述置样部,将所述置样部置于所述检测位置上。The transfer part releases the sample setting part, and places the sample setting part on the detection position.
- 根据权利要求1所述的加样混匀方法,其中,在位于所述悬空位置时,所述置样部在所述移载部的作用下而处于悬空状态。The sample adding and mixing method according to claim 1, wherein when in the suspended position, the sample placement part is in a suspended state under the action of the transfer part.
- 根据权利要求5所述的加样混匀方法,其中,还包括:The sample adding and mixing method according to claim 5, further comprising:检测所述置样部是否置于所述移载部上;Detecting whether the sample placement part is placed on the transfer part;在所述置样部置于所述移载部上时,获取所述置样部的当前位置;When the sample setting part is placed on the transfer part, acquiring the current position of the sample setting part;根据所述置样部的当前位置,通过所述移载部来控制所述置样部的所处状态。According to the current position of the sample setting part, the state of the sample setting part is controlled by the transfer part.
- 根据权利要求5所述的加样混匀方法,其中,所述置样部的所处状态为下述状态中的其中一种:所述移动状态、所述混匀状态、所述悬空状态、同时处于所述移动状态和所述混匀状态的状态、同时处于所述混匀状态与所述悬空状态的状态。The sample adding and mixing method according to claim 5, wherein the state of the sample setting part is one of the following states: the moving state, the mixing state, the suspended state, It is in the state of the moving state and the mixing state at the same time, and in the state of the mixing state and the suspended state at the same time.
- 根据权利要求1所述的加样混匀方法,其中,所述待加物为样本和/或试剂;The sample adding and mixing method according to claim 1, wherein the object to be added is a sample and/or a reagent;所述加样部向所述置样部添加待加物的步骤包括:The step of adding an object to be added by the sample adding part to the sample placing part includes:所述加样部与所述置样部彼此靠近;The sample adding part and the sample placing part are close to each other;所述加样部在加样位置上将所述待加物添加至所述置样部;The sample adding part adds the object to be added to the sample placing part at the sample adding position;在加样完成后,所述加样部与所述置样部彼此远离。After the sample application is completed, the sample application part and the sample placement part are far away from each other.
- 根据权利要求8所述的加样混匀方法,其中,所述加样部与所述置样部彼此靠近的步骤与所述将置样部置于悬空位置上的步骤同时执行。The sample adding and mixing method according to claim 8, wherein the step of bringing the sample adding part and the sample placing part close to each other is performed simultaneously with the step of placing the sample placing part in a suspended position.
- 根据权利要求8所述的加样混匀方法,其中,处于加样位置的所述加样部在向所述置样部添加所述待加物后,所述置样部内的液面与所述加样部之间处于第一状态或第二状态;The sample adding and mixing method according to claim 8, wherein after the sample adding part at the sample adding position adds the object to be added to the sample placing part, the liquid level in the sample placing part is The sample adding parts are in the first state or the second state;所述第一状态为所述加样部的触液位置与所述置样部内的液面位于同一高度水平,并相互接触;所述第二状态为所述加样部的触液位置间隔设置于所述置样部内的液面上方,且与所述置样部内的液面之间的距离小于预设距离。The first state is that the liquid contact position of the sample application part and the liquid surface in the sample placement part are at the same height level and are in contact with each other; the second state is that the liquid contact position of the sample application part is arranged at intervals It is above the liquid surface in the sample placing part and the distance from the liquid surface in the sample placing part is less than a predetermined distance.
- 根据权利要求1所述的加样混匀方法,其中,所述将置样部置于悬空位置上的步骤包括:The sample adding and mixing method according to claim 1, wherein the step of placing the sample setting part on a suspended position comprises:所述移载部将所述置样部由放置位置移动至所述悬空位置;The transfer part moves the sample placement part from the placement position to the suspended position;其中,所述放置位置与所述检测位置之间的距离大于所述悬空位置与所述检测位置之间的距离。Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position.
- 根据权利要求11所述的加样混匀方法,其中,所述悬空位置设置成使 得所述放置位置与所述检测位置之间的距离、所述悬空位置与所述检测位置之间的距离以及所述放置位置与所述悬空位置之间的距离之和最短。The sample adding and mixing method according to claim 11, wherein the suspended position is set such that the distance between the placement position and the detection position, the distance between the suspended position and the detection position, and The sum of the distances between the placement position and the suspended position is the shortest.
- 根据权利要求11所述的加样混匀方法,其中,所述移载部将所述置样部由放置位置移动至悬空位置的步骤包括:The sample adding and mixing method according to claim 11, wherein the step of the transferring part to move the sample placing part from the placing position to the suspended position comprises:所述移载部对位于所述放置位置上的所述置样部进行夹取;The transfer part clamps the sample placement part located at the placement position;所述移载部将所述置样部移送至所述悬空位置。The transfer part transfers the sample placement part to the suspended position.
- 根据权利要求13所述的加样混匀方法,其中,所述移载部为机械手、手指气缸、夹爪中的至少一种。The sample adding and mixing method according to claim 13, wherein the transfer part is at least one of a manipulator, a finger cylinder, and a clamping jaw.
- 根据权利要求11所述的加样混匀方法,其中,所述悬空位置位于所述放置位置上方、所述检测位置上方或者所述放置位置与所述检测位置之间;The sample adding and mixing method according to claim 11, wherein the suspended position is located above the placement position, above the detection position, or between the placement position and the detection position;所述放置位置是在置于所述悬空位置之前,所述置样部所放置的位置。The placement position is the position where the sample placement part is placed before being placed in the suspended position.
- 根据权利要求1所述的加样混匀方法,其中,所述通过移载部对所述置样部进行混匀操作的步骤为:The sample adding and mixing method according to claim 1, wherein the step of performing a mixing operation on the sample setting part by a transfer part is:所述移载部对所夹持的所述置样部施加能够使得所述置样部执行混匀动作的驱动力;The transfer part applies a driving force to the clamped sample setting part that enables the sample setting part to perform a mixing action;所述驱动力包括转动力、摆动力和振动力中的至少一种。The driving force includes at least one of rotation force, swing force, and vibration force.
- 一种加样混匀装置,其中,所述加样混匀装置包括:A sample adding and mixing device, wherein the sample adding and mixing device comprises:加样部,用于在置样部至于悬空位置上时,向所述置样部内添加待加物;The sample adding part is used for adding objects to be added into the sample placing part when the sample placing part is in the suspended position;移载部,用于将所述置样部由放置位置移动至所述悬空位置,并对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到所述待测物,并将所述置样部移至检测位置以对所述待测物进行检测;The transfer part is used to move the sample setting part from the placing position to the suspended position, and perform a mixing operation on the sample setting part, so that the additives in the sample setting part are mixed uniformly to obtain the The object to be tested, and move the sample placement part to the detection position to detect the object to be tested;其中,所述放置位置与所述检测位置之间的距离大于所述悬空位置与所述检测位置之间的距离。Wherein, the distance between the placement position and the detection position is greater than the distance between the suspended position and the detection position.
- 根据权利要求17所述的加样混匀装置,还包括:The sample adding and mixing device according to claim 17, further comprising:置样部,用于放置所述待加物。The sample setting part is used to place the object to be added.
- 根据权利要求17所述的加样混匀装置,还包括:The sample adding and mixing device according to claim 17, further comprising:第一加样驱动部,用于驱动所述加样部沿预设轨迹运动;The first sample adding driving part is used to drive the sample adding part to move along a preset trajectory;第二加样驱动部,用于驱动所述加样部实现所述待加物的吸取或添加操作。The second sample adding driving part is used to drive the sample adding part to realize the suction or adding operation of the object to be added.控制部,分别与所述第一加样驱动部、所述第二加样驱动部及所述移载部连接,用于对所述第一加样驱动部、所述第二加样驱动部和所述移载部进行控制。The control unit is respectively connected with the first sample adding drive unit, the second sample adding drive unit, and the transfer unit, and is used for controlling the first sample adding drive unit and the second sample adding drive unit. Control with the transfer part.
- 一种非临时性计算机可读存储介质,其中,所述非临时性计算机可读存储介质存储有计算机程序,所述计算机程序被处理器执行时,使得所述处理器执行如下步骤:A non-transitory computer-readable storage medium, wherein the non-transitory computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the processor executes the following steps:通过移栽部将置样部置于悬空位置上;Place the sample placement part in a suspended position through the transplanting part;加样部向所述置样部内添加待加物;The sample adding part adds an object to be added into the sample placing part;在所述置样部达到检测位置之前,通过移载部对所述置样部进行混匀操作,以使得所述置样部内的添加物混匀而得到待测物。Before the sample setting part reaches the detection position, a mixing operation is performed on the sample setting part through the transfer part, so that the additives in the sample setting part are mixed uniformly to obtain the test object.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911206958.5 | 2019-11-29 | ||
CN201911206958.5A CN112871029B (en) | 2019-11-29 | 2019-11-29 | Sample adding and mixing method, sample adding and mixing device, computer storage medium, sample analysis method and sample analysis device |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2021104499A1 true WO2021104499A1 (en) | 2021-06-03 |
Family
ID=76039166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2020/132465 WO2021104499A1 (en) | 2019-11-29 | 2020-11-27 | Sample adding and mixing method and apparatus, and non-transitory computer readable storage medium |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN112871029B (en) |
WO (1) | WO2021104499A1 (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002001137A (en) * | 2000-06-15 | 2002-01-08 | Japan Tobacco Inc | Shaking device |
US20090196793A1 (en) * | 2008-02-06 | 2009-08-06 | Kabushiki Kaisha Toshiba | Automatic analyzing apparatus |
CN202522565U (en) * | 2011-12-29 | 2012-11-07 | 福州艾迪康医学检验所有限公司 | Blood analyzer |
CN103364255A (en) * | 2012-03-29 | 2013-10-23 | 深圳市开立科技有限公司 | Blending device for hematology analyzer |
CN105628948A (en) * | 2016-03-04 | 2016-06-01 | 深圳普门科技有限公司 | High-speed C reactive protein analyzer and analyzing method thereof |
CN209438627U (en) * | 2018-12-24 | 2019-09-27 | 徐建顺 | A kind of blood pipetting device of thrombus detection machine |
CN209542622U (en) * | 2019-02-20 | 2019-10-25 | 重庆科斯迈生物科技有限公司 | Gripper system of chemiluminescence immunoassay analyzer |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08178824A (en) * | 1994-12-21 | 1996-07-12 | Toa Medical Electronics Co Ltd | Particle measuring apparatus |
EP2860528B1 (en) * | 2012-06-11 | 2018-01-03 | Hitachi High-Technologies Corporation | Automatic analysis apparatus |
CN106680476B (en) * | 2017-01-22 | 2018-08-03 | 英科新创(厦门)科技有限公司 | A kind of immuno-chromatography detection device for secretion sample |
CN107014990A (en) * | 2017-05-15 | 2017-08-04 | 深圳麦科田生物医疗技术有限公司 | Full-automatic thrombus elastic force measuring device |
CN207263759U (en) * | 2017-09-29 | 2018-04-20 | 深圳市新产业生物医学工程股份有限公司 | Chemiluminescence detector and evenly mixing device |
CN109883800B (en) * | 2019-02-18 | 2021-12-24 | 深圳唯公生物科技有限公司 | Sample mixing and moving mechanism and method thereof |
-
2019
- 2019-11-29 CN CN201911206958.5A patent/CN112871029B/en active Active
-
2020
- 2020-11-27 WO PCT/CN2020/132465 patent/WO2021104499A1/en active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002001137A (en) * | 2000-06-15 | 2002-01-08 | Japan Tobacco Inc | Shaking device |
US20090196793A1 (en) * | 2008-02-06 | 2009-08-06 | Kabushiki Kaisha Toshiba | Automatic analyzing apparatus |
CN202522565U (en) * | 2011-12-29 | 2012-11-07 | 福州艾迪康医学检验所有限公司 | Blood analyzer |
CN103364255A (en) * | 2012-03-29 | 2013-10-23 | 深圳市开立科技有限公司 | Blending device for hematology analyzer |
CN105628948A (en) * | 2016-03-04 | 2016-06-01 | 深圳普门科技有限公司 | High-speed C reactive protein analyzer and analyzing method thereof |
CN209438627U (en) * | 2018-12-24 | 2019-09-27 | 徐建顺 | A kind of blood pipetting device of thrombus detection machine |
CN209542622U (en) * | 2019-02-20 | 2019-10-25 | 重庆科斯迈生物科技有限公司 | Gripper system of chemiluminescence immunoassay analyzer |
Also Published As
Publication number | Publication date |
---|---|
CN112871029B (en) | 2022-06-14 |
CN112871029A (en) | 2021-06-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Alexovič et al. | Achievements in robotic automation of solvent extraction and related approaches for bioanalysis of pharmaceuticals | |
US11898947B2 (en) | Diagnostic method and device performing the same | |
US8951803B2 (en) | Sample analyzer and sample analyzing method | |
US9448247B2 (en) | Blood sample processing apparatus and blood sample processing method | |
JP6320535B2 (en) | Automatic analyzer | |
EP2541257A2 (en) | A dispenser device for and a method of dispensing a substance onto a substrate | |
US4231989A (en) | Multichannel system for the handling of immobilized biologically active substances | |
JP5393610B2 (en) | Nucleic acid analyzer | |
JP2009019922A (en) | Chemical analyzer | |
JPH10260118A (en) | Automatic extraction device and automatic concentration-measuring apparatus for component substance in liquid sample | |
JP7057813B2 (en) | Pipetting unit and pipetting method for closed liquid containers | |
JP2010502988A (en) | Micro sample cup rack adapter | |
US6709872B1 (en) | Method and apparatus for dispensing low nanoliter volumes of liquid while minimizing waste | |
WO2021104499A1 (en) | Sample adding and mixing method and apparatus, and non-transitory computer readable storage medium | |
JP2013156256A (en) | Method for heating liquid of constant volume in heated pipette needle part | |
WO2019040894A1 (en) | Substance dispense system for biological sample analysis instrument | |
CN109975569B (en) | Control method and system of chemiluminescence detector and chemiluminescence detector | |
JP5046586B2 (en) | Automatic analyzer | |
JP2019124523A (en) | Stirrer and specimen smear device including the same | |
JP2012127974A (en) | Chemical analyzer | |
CN218710491U (en) | Molecular pretreatment device and sample analyzer | |
CN112881739A (en) | Sample adding method and device, computer storage medium, sample analysis method and device | |
JP2000083650A (en) | Automatic test device for testing enzymatic reaction | |
JP2005249585A (en) | Autoanalyzer and analysis method | |
CN210376370U (en) | Device based on little flow control quantitative determination allergen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 20894332 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 20894332 Country of ref document: EP Kind code of ref document: A1 |