WO2021050013A1 - A tissue disaggregation, homogenization, photoactivation device - Google Patents

A tissue disaggregation, homogenization, photoactivation device Download PDF

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Publication number
WO2021050013A1
WO2021050013A1 PCT/TR2019/050745 TR2019050745W WO2021050013A1 WO 2021050013 A1 WO2021050013 A1 WO 2021050013A1 TR 2019050745 W TR2019050745 W TR 2019050745W WO 2021050013 A1 WO2021050013 A1 WO 2021050013A1
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WO
WIPO (PCT)
Prior art keywords
homogenization
photoactivation
kit
disaggregation
set forth
Prior art date
Application number
PCT/TR2019/050745
Other languages
French (fr)
Inventor
Seyithan HOKENEK
Original Assignee
Global Biyomedikal Saglik Hizmetleri Sanayi Ve Ticaret Limited Sirketi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Global Biyomedikal Saglik Hizmetleri Sanayi Ve Ticaret Limited Sirketi filed Critical Global Biyomedikal Saglik Hizmetleri Sanayi Ve Ticaret Limited Sirketi
Priority to US17/642,616 priority Critical patent/US20220341824A1/en
Priority to EP19849050.0A priority patent/EP4028744A1/en
Priority to PCT/TR2019/050745 priority patent/WO2021050013A1/en
Publication of WO2021050013A1 publication Critical patent/WO2021050013A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

Definitions

  • the invention presented hereby generally concerns systems, methods and devices for analysis of biological material such as samples from a living organism on the cellular level, called cytometry. Disclosed invention more specifically falls within the technical areas of cytometry for purposes of diagnosis and treatment such as blood tests, blood counts, research and medical diagnostics.
  • Cytometry refers to methods for measurement of certain properties and characteristics of cells such as cell size, cell count, morphology and genetic content.
  • a common usage is found in medical industry, particularly in hematology where size and quantity of particles and cells make up the blood are determined for various purposes including diagnostic and treatment-oriented ones.
  • homogenization attempts to bring a sample to a state where all fractions of the sample have equal composition. Homogenization is an important step in analytical testing because through removing sample matrices, the shielded analytes are made accessible to the probes and/or instruments that are employed in their detection. Additionally, by spreading the analytes uniformly throughout the sample, homogenization aids in the efficient extraction of analytes in analytical procedures that involve analyte extraction prior to detection.
  • a device in the art albeit capable of disaggregation and separation, does not possess timing or revolution adjustment options.
  • said device is also incapable of delivering processes of homogenization which require different revolution characteristics.
  • the device also, as it comprises kits that contain the separation material as a component, allows only for atmospheric disaggregation i.e. that takes place in contact with air; which may cause contamination. Also lacking are a software support, registerable protocols or photoactivation capabilities.
  • Another device documented in the art addresses these problems in part with regards to timing and protocol registering; however still fails to offer a means for adjusting revolution characteristics.
  • Said device is centered around homogenization systems that revolve at a steady, unalterable rate i.e. 4000 rpm which causes overheating in components of homogenization kits which contain material to be homogenized, causing them to degrade.
  • Said device like the one before, is also not capable of photoactivation.
  • US 9962717 B1 discloses a multiplicity of instruments and methods for performing both sample homogenization and sample clarification by centrifugation with a single instrument without transferring the sample to a new sample container and without removing or repositioning the sample container within the instrument.
  • Some embodiments of the instrument may automatically perform centrifugation after homogenization, also the In other embodiments, the instrument may perform both homogenization and centrifugation in a simultaneous manner.
  • the considerations regarding technologies of disaggregation/separation, homogenization and photoactivation is rate of revolution for effectuating separation and homogenization processes; heat exchange properties to bypass overheating due to high rpm; integrity of material and kits holding thereof, all of which require to be addressed for improvement. It is important to point out that overheating persist especially in homogenization processes, where regularly utilized plastic parts perform poorly. Moving parts, as well as other non-moving parts that make up the devices are prone to wear and damage over time.
  • Primary object of the disclosed invention is to present a device for tissue disaggregation and separation.
  • Another object of the disclosed invention is to present a device for homogenization.
  • Another object of the disclosed invention is to present a device for photoactivation.
  • Another object of the disclosed invention is to present a device obviating the need for fan driven cooling after processes of disaggregation, separation and homogenization.
  • proposed invention comprises an aluminium body with innate heat sink properties against deformation and overheating as a design feature; enabling a stable setting for very high rates of revolutions-per-minute which may well exceed a rate of four thousand, producing excess heat. Said property also alleviates the need for active cooling via fans.
  • processes of disaggregation and homogenization may be handled in a simultaneous manner at will.
  • a photoactivation means is present, rendering simultaneous utility of photoactivation and homogenization possible, eliminating extra steps for preparation. Protocols of both processes are registerable and adjustable in parameters i.e. rate of revolution and duration of process overall; executed through a electronic control unit capable of storing and overseeing said protocols.
  • Fig. 1 demonstrates the exploded top perspective view of the device according to the disclosed invention.
  • Fig. 2 demonstrates the calibration/cooling mount chamber comprising kits and the photoactivation means according to the disclosed invention.
  • Fig. 3 demonstrates the top perspective view of the chamber formed by the bottom mount and the kit adapter in the open position of the device according to the disclosed invention.
  • proposed invention compact and efficient systems/devices with features contributing to simultaneous occurrence of both tissue disaggregation and homonegization processes are disclosed.
  • said processes of disaggregation/separation and homogenization further merge with the ability to induce photoactivation via a set of different oprions among which light-emitting diode (LED) usage occurs.
  • proposed invention comprises an aluminium body with innate heat sink properties against deformation and overheating as a design feature; enabling a stable setting for very high rates of revolutions-per-minute which may well exceed a rate of four thousand, producing excess heat. Said property also alleviates the need for active cooling via fans.
  • processes of disaggregation and homogenization may be handled in a simultaneous manner at will.
  • a photoactivation means is present, rendering simultaneous utility of photoactivation and homogenization possible, eliminating extra steps for preparation. Protocols of both processes are registerable and adjustable in parameters i.e. rate of revolution and duration of process overall; executed through a electronic control unit (31) capable of storing and overseeing said protocols.
  • top mount (11) and bottom mount (26) are brought together with at least one, preferably two hinges (25) at corresponding sides thereof, whereas the opposite sides ensure tight closure with a magnet (12) at partial protrusion from said top mount (11) and a magnet holder (24) with structural relationship with said bottom mount (26) in a closed position.
  • said top mount (11) houses two motors, namely a high RPM motor (13) and a low RPM motor (14) both of which are fixed into position with a motor mount (16) letting drive couplings of said motors (13, 14) retain full rotatability.
  • Said low and high RPM motors (13, 14) are controllable in speed, i.e.
  • disaggregation/separation methods may be employed due to the adjustable RPM feature, such as fatty tissue, hair follicles, connective tissue including but not limited to cartilage, skin etc.
  • adjustable RPM feature such as fatty tissue, hair follicles, connective tissue including but not limited to cartilage, skin etc.
  • said bottom mount (26) comprises a calibration and cooling mount (22) as a space confined within inner walls of said bottom mount (26) and above which a kit mount (22) that hosts one low RPM kit (17) and one high RPM kit (18).
  • Said calibration and cooling mount (22) comprises, according to at least one embodiment, a photoactivation means (23).
  • Said photoactivation means (23) further comprises a vertical and upwards light emission setting, which is in one particular embodiment a light-emitting diode (LED).
  • Embodiments of the invention comprise an electronic control unit (31) configured to execute tasks of homogenization, separation and photoactivation via commanding high RPM motor (13) and low RPM motor (14).
  • Said ECU (31) may control said high and low RPM motors (13, 14) in aspects of duration and revolutions-per-minute and thus effectuate different regimes of operation. Homogenization and homogenization merged with photoactivation are distinct, different operation regimes, i.e. protocols.
  • a homogenization process may last thirty seconds with a high rate of revolution.
  • joint homogenization- photoactivation process may last twenty to thirty minutes with a low rate of revolution. Purpose for this is to retain material homogeneity within the timeframe required for photoactivation to be effectuated.
  • homogenization process is executed so that the kit and the material are not spoiled, enabled by the aforementioned adjustability feature of both the high RPM motor (13) and the low RPM motor (14).
  • a rate of revolution approximately between 1500-3000 RPM is deemed appropriate.
  • the rate of revolution for the high RPM motor (13) is set to be 2000 RPM.
  • a protocol for homogenization process is employed such that it runs for thirty seconds (30 s) at 2000 RPM.
  • a protocol for separation is employed such that it runs at the low RPM kit (17) for two minutes (120 s) at 80 RPM. Said protocol is specifically employed for hair follicles.
  • a protocol for separation is employed such that it runs at the high RPM kit
  • a protocol for separation is employed such that it runs at the low RPM kit (17) or the high RPM kit (18) for three minutes (180 s) at 120 RPM.
  • Said protocol is specifically employed for cartilage tissue.
  • a protocol for homogenization is employed such that it runs for thirty seconds (30s) at 2000 RPM.
  • Said embodiment is configured to homogenize a mixture of hair follicle origin.
  • a protocol for homogenization is employed such that it runs for thirty seconds (30s) at 2000 RPM.
  • Said embodiment is configured to homogenize a mixture of cartilage origin.
  • a protocol for joint homogenization-photoactivation is employed such that it runs for a period between 15 and 20 minutes at 50 RPM.
  • Said embodiment is configured for mixture(s) of hair follicle, fatty tissue and cartilage origin.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a disaggregation and homogenization device also capable of photoactivation to improve heat resilience, photoactivation efficiency and simultaneous processing. In the disclosed invention processes of disaggregation and homogenization may be handled in a simultaneous manner at will. Moreover, in various embodiments of the disclosed invention, a photoactivation means is present, rendering simultaneous utility of photoactivation and homogenization possible, eliminating extra steps for preparation. Protocols of both processes are registerable and adjustable in parameters i.e. rate of revolution and duration of process overall; executed through an electronic control unit capable of storing and executing said protocols.

Description

A TISSUE DISAGGREGATION, HOMOGENIZATION AND PHOTOACTIVATION DEVICE
Technical Field of the Present Invention
The invention presented hereby generally concerns systems, methods and devices for analysis of biological material such as samples from a living organism on the cellular level, called cytometry. Disclosed invention more specifically falls within the technical areas of cytometry for purposes of diagnosis and treatment such as blood tests, blood counts, research and medical diagnostics. Prior Art/Background of the Present Invention
Cytometry refers to methods for measurement of certain properties and characteristics of cells such as cell size, cell count, morphology and genetic content. A common usage is found in medical industry, particularly in hematology where size and quantity of particles and cells make up the blood are determined for various purposes including diagnostic and treatment-oriented ones. For different applications, homogenization attempts to bring a sample to a state where all fractions of the sample have equal composition. Homogenization is an important step in analytical testing because through removing sample matrices, the shielded analytes are made accessible to the probes and/or instruments that are employed in their detection. Additionally, by spreading the analytes uniformly throughout the sample, homogenization aids in the efficient extraction of analytes in analytical procedures that involve analyte extraction prior to detection. Most methods and devices employing said methods still have technical shortcomings and problems yet to be addressed in full. For instance, a device in the art, albeit capable of disaggregation and separation, does not possess timing or revolution adjustment options. In addition, said device is also incapable of delivering processes of homogenization which require different revolution characteristics. The device also, as it comprises kits that contain the separation material as a component, allows only for atmospheric disaggregation i.e. that takes place in contact with air; which may cause contamination. Also lacking are a software support, registerable protocols or photoactivation capabilities.
Another device documented in the art addresses these problems in part with regards to timing and protocol registering; however still fails to offer a means for adjusting revolution characteristics. Said device is centered around homogenization systems that revolve at a steady, unalterable rate i.e. 4000 rpm which causes overheating in components of homogenization kits which contain material to be homogenized, causing them to degrade. Said device, like the one before, is also not capable of photoactivation.
US 9962717 B1 discloses a multiplicity of instruments and methods for performing both sample homogenization and sample clarification by centrifugation with a single instrument without transferring the sample to a new sample container and without removing or repositioning the sample container within the instrument. Some embodiments of the instrument may automatically perform centrifugation after homogenization, also the In other embodiments, the instrument may perform both homogenization and centrifugation in a simultaneous manner. The considerations regarding technologies of disaggregation/separation, homogenization and photoactivation is rate of revolution for effectuating separation and homogenization processes; heat exchange properties to bypass overheating due to high rpm; integrity of material and kits holding thereof, all of which require to be addressed for improvement. It is important to point out that overheating persist especially in homogenization processes, where regularly utilized plastic parts perform poorly. Moving parts, as well as other non-moving parts that make up the devices are prone to wear and damage over time.
Objects of the Present Invention Primary object of the disclosed invention is to present a device for tissue disaggregation and separation.
Another object of the disclosed invention is to present a device for homogenization.
Another object of the disclosed invention is to present a device for photoactivation.
Another object of the disclosed invention is to present a device obviating the need for fan driven cooling after processes of disaggregation, separation and homogenization.
Yet another object of the disclosed invention is to present a device comprising a strong, metal body. Yet another object of the disclosed invention is to present a device capable of simultaneously delivering disaggregation, separation and homogenization on demand. Yet another object of the disclosed invention is to present a device capable of providing timewise and ratewise adjustable processes of disaggregation and separation.
Summary of the Present Invention
In proposed invention, compact and efficient systems/devices with features contributing to simultaneous occurrence of both tissue disaggregation and homonegization processes are disclosed. In the disclosed invention, said processes of disaggregation/separation and homogenization further merge with the ability to induce photoactivation via a set of different options among which light-emitting diode (LED) usage occurs.
To offer an instrumental setting solid and durable against the wear and tear regularly occurring during said processes, proposed invention comprises an aluminium body with innate heat sink properties against deformation and overheating as a design feature; enabling a stable setting for very high rates of revolutions-per-minute which may well exceed a rate of four thousand, producing excess heat. Said property also alleviates the need for active cooling via fans.
In different embodiments of the disclosed invention, processes of disaggregation and homogenization may be handled in a simultaneous manner at will. Moreover, in various embodiments of the disclosed invention, a photoactivation means is present, rendering simultaneous utility of photoactivation and homogenization possible, eliminating extra steps for preparation. Protocols of both processes are registerable and adjustable in parameters i.e. rate of revolution and duration of process overall; executed through a electronic control unit capable of storing and overseeing said protocols.
Brief Description of the Figures of the Present Invention
Accompanying figures are given solely for the purpose of exemplifying an rpm-adjustable disaggregation and homogenization device also capable of photoactivation, whose advantages over prior art were outlined above and will be explained in brief hereinafter.
The figures are not meant to delimit the scope of protection as identified in the claims nor should they be referred to alone in an effort to interpret the scope identified in said claims without recourse to the technical disclosure in the description.
Fig. 1 demonstrates the exploded top perspective view of the device according to the disclosed invention.
Fig. 2 demonstrates the calibration/cooling mount chamber comprising kits and the photoactivation means according to the disclosed invention.
Fig. 3 demonstrates the top perspective view of the chamber formed by the bottom mount and the kit adapter in the open position of the device according to the disclosed invention. Detailed Description of the Present Invention
10) Top lid
11) Top mount
12) Magnet
13) High RPM motor
14) Low RPM motor
15) Drive coupling
16) Motor mount
17) Low RPM kit
18) High RPM kit
19) Kit adapter
20) Kit mount
21) Kit mount support
22) Calibration and cooling mount
23) Photoactivation means
24) Magnet holding means
25) Hinge
26) Bottom mount
27) Bottom lid
28) Power supply
29) Frontal cover
30) Side cover
31) ECU
In proposed invention, compact and efficient systems/devices with features contributing to simultaneous occurrence of both tissue disaggregation and homonegization processes are disclosed. In the disclosed invention, said processes of disaggregation/separation and homogenization further merge with the ability to induce photoactivation via a set of different oprions among which light-emitting diode (LED) usage occurs. To offer an instrumental setting solid and durable against the wear and tear regularly occurring during said processes, proposed invention comprises an aluminium body with innate heat sink properties against deformation and overheating as a design feature; enabling a stable setting for very high rates of revolutions-per-minute which may well exceed a rate of four thousand, producing excess heat. Said property also alleviates the need for active cooling via fans.
In different embodiments of the disclosed invention, processes of disaggregation and homogenization may be handled in a simultaneous manner at will. Moreover, in various embodiments of the disclosed invention, a photoactivation means is present, rendering simultaneous utility of photoactivation and homogenization possible, eliminating extra steps for preparation. Protocols of both processes are registerable and adjustable in parameters i.e. rate of revolution and duration of process overall; executed through a electronic control unit (31) capable of storing and overseeing said protocols.
According to at least one embodiment of the proposed invention, top mount (11) and bottom mount (26) are brought together with at least one, preferably two hinges (25) at corresponding sides thereof, whereas the opposite sides ensure tight closure with a magnet (12) at partial protrusion from said top mount (11) and a magnet holder (24) with structural relationship with said bottom mount (26) in a closed position. According to at least one embodiment of the proposed invention, said top mount (11) houses two motors, namely a high RPM motor (13) and a low RPM motor (14) both of which are fixed into position with a motor mount (16) letting drive couplings of said motors (13, 14) retain full rotatability. Said low and high RPM motors (13, 14) are controllable in speed, i.e. rate of revolution, determination of which in turn enables different types of tissues comprising different cells of various characteristics to be subjected to disaggregation. According to at least one embodiment of the proposed invention, different disaggregation/separation methods may be employed due to the adjustable RPM feature, such as fatty tissue, hair follicles, connective tissue including but not limited to cartilage, skin etc. However, it is to be noted that at any rate, which is also characteristic considering their different purposes.
According to at least one embodiment of the proposed invention, said bottom mount (26) comprises a calibration and cooling mount (22) as a space confined within inner walls of said bottom mount (26) and above which a kit mount (22) that hosts one low RPM kit (17) and one high RPM kit (18). Said calibration and cooling mount (22) comprises, according to at least one embodiment, a photoactivation means (23). Said photoactivation means (23) further comprises a vertical and upwards light emission setting, which is in one particular embodiment a light-emitting diode (LED). Said photoactivation means (23) as an upwards positioned light emission setting enables, contrary to different photoactivation methods and devices in the art, ensures that light emitted by said means covers a wider effective range for reaching cells further away from a given light source, in turn increasing photoactivation efficiency greatly. Embodiments of the invention comprise an electronic control unit (31) configured to execute tasks of homogenization, separation and photoactivation via commanding high RPM motor (13) and low RPM motor (14). Said ECU (31) may control said high and low RPM motors (13, 14) in aspects of duration and revolutions-per-minute and thus effectuate different regimes of operation. Homogenization and homogenization merged with photoactivation are distinct, different operation regimes, i.e. protocols. According to one embodiment, a homogenization process may last thirty seconds with a high rate of revolution. According to one embodiment, joint homogenization- photoactivation process may last twenty to thirty minutes with a low rate of revolution. Purpose for this is to retain material homogeneity within the timeframe required for photoactivation to be effectuated.
According to at least one embodiment of the proposed invention, homogenization process is executed so that the kit and the material are not spoiled, enabled by the aforementioned adjustability feature of both the high RPM motor (13) and the low RPM motor (14). In an embodiment, a rate of revolution approximately between 1500-3000 RPM is deemed appropriate. In a particular embodiment, the rate of revolution for the high RPM motor (13) is set to be 2000 RPM.
According to at least one embodiment of the disclosed invention, a protocol for homogenization process is employed such that it runs for thirty seconds (30 s) at 2000 RPM.
According to at least one embodiment of the disclosed invention, a protocol for separation is employed such that it runs at the low RPM kit (17) for two minutes (120 s) at 80 RPM. Said protocol is specifically employed for hair follicles.
According to at least one embodiment of the disclosed invention, a protocol for separation is employed such that it runs at the high RPM kit
(18) for twelve minutes (720 s) at 100 RPM. Said protocol is specifically employed for fat tissue.
According to at least one embodiment of the disclosed invention, a protocol for separation is employed such that it runs at the low RPM kit (17) or the high RPM kit (18) for three minutes (180 s) at 120 RPM. Said protocol is specifically employed for cartilage tissue.
According to at least one embodiment of the disclosed invention, a protocol for homogenization is employed such that it runs for thirty seconds (30s) at 2000 RPM. Said embodiment is configured to homogenize a mixture of hair follicle origin.
According to at least one embodiment of the disclosed invention, a protocol for homogenization is employed such that it runs for thirty seconds (30s) at 2000 RPM. Said embodiment is configured to homogenize a mixture of cartilage origin.
According to at least one embodiment of the disclosed invention, a protocol for joint homogenization-photoactivation is employed such that it runs for a period between 15 and 20 minutes at 50 RPM. Said embodiment is configured for mixture(s) of hair follicle, fatty tissue and cartilage origin.

Claims

1) A device for disaggregation and homogenization on histoid and cellular levels comprising at least one high RPM motor (13) for homogenization and one low RPM motor (14) for disaggregation characterized in that; said device further comprises a photoactivation means (23) situated horizontally beneath a low RPM kit (17) and a high RPM kit (18) emitting light vertically towards said kits in a bottom-up lighting configuration for deep and efficient photoactivation; and, said device further comprises an electronic control unit (31) configured to execute protocols containing predefined sets of actions such that a mixture situated inside a kit can be simulataneously subjected to red, near-IR or IR light while being homogenized.
2) A device for disaggregation and homogenization on histoid and cellular levels as set forth in Claim 1 characterized in that said low RPM kit (17) and high RPM kit (18) are at least partially translucent or semi-transparent to facilitate photoactivation.
3) A device for disaggregation and homogenization on histoid and cellular levels as set forth in Claim 1 characterized in that said low RPM kit (17) and high RPM kit (18) are fully transparent to facilitate photoactivation.
4) A device for disaggregation and homogenization on histoid and cellular levels as set forth in any preceding Claim characterized in that said photoactivation means (23) is configured to radiate in the range from 600 nm to 750 nm. 5) A device for disaggregation and homogenization on histoid and cellular levels as set forth in Claim 1 characterized in that said photoactivation means (23) is configured to radiate in the range from 750 nm to 1 mm.
6) A device for disaggregation and homogenization on histoid and cellular levels as set forth in any preceding Claim characterized in that said device further comprises a casing preferably made of aluminium, with air ducts facilitating cooling.
7) A method for processing samples and mixtures into serums comprising distinct steps of; mixture preparation; where mixtures are produced using samples of different histoid and cellular character(s), kit preparation; where produced mixture is rendered process- compatible by insertion into low or high RPM kits, homogenization and photoactivation; where a mixture situated inside a kit is subjected to red, near-IR or IR light while being homogenized, coming out readily deployable.
8) A method for processing samples and mixtures into serums as set forth in Claim 7, characterized in that said homogenization and photoactivation step is durationwise controllable, whereby rehomogenization due to precipitation is prevented.
9) A method for processing samples and mixtures into serums as set forth in Claim 7 and 8, characterized in that said homogenization and photoactivation step is controllable in terms of rate of revolution whereby kit deformation is prevented. 10) A method for processing samples and mixtures into serums as set forth in any preceding Claim, characterized in that the mixture obtained after said homogenization and photoactivation comprises photoactivated stem cells.
PCT/TR2019/050745 2019-09-11 2019-09-11 A tissue disaggregation, homogenization, photoactivation device WO2021050013A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US17/642,616 US20220341824A1 (en) 2019-09-11 2019-09-11 Tissue Disaggregation, Homogenization, Photoactivation Device
EP19849050.0A EP4028744A1 (en) 2019-09-11 2019-09-11 A tissue disaggregation, homogenization, photoactivation device
PCT/TR2019/050745 WO2021050013A1 (en) 2019-09-11 2019-09-11 A tissue disaggregation, homogenization, photoactivation device

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PCT/TR2019/050745 WO2021050013A1 (en) 2019-09-11 2019-09-11 A tissue disaggregation, homogenization, photoactivation device

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Citations (3)

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Publication number Priority date Publication date Assignee Title
US9962717B1 (en) 2017-07-31 2018-05-08 Mp Biomedicals, Llc Instrument for automated sample preparation by combination homogenization and clarification
US20190079063A1 (en) * 2016-03-15 2019-03-14 Dots Technology Corp. Systems and methods for allergen detection
CN110174369A (en) * 2019-06-14 2019-08-27 深圳市雷凌广通技术研发有限公司 It is a kind of that clean pesticide residue detection device is convenient for based on spectroscopic methodology

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190079063A1 (en) * 2016-03-15 2019-03-14 Dots Technology Corp. Systems and methods for allergen detection
US9962717B1 (en) 2017-07-31 2018-05-08 Mp Biomedicals, Llc Instrument for automated sample preparation by combination homogenization and clarification
CN110174369A (en) * 2019-06-14 2019-08-27 深圳市雷凌广通技术研发有限公司 It is a kind of that clean pesticide residue detection device is convenient for based on spectroscopic methodology

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Title
""Chromophores (Photoacceptors) for Low-Level Laser Therapy" in "Handbook of Photomedicine"", 22 October 2013, ISBN: 978-1-4398-8469-0, article TIINA I. KARU: ""Chromophores (Photoacceptors) for Low-Level Laser Therapy" in "Handbook of Photomedicine"", XP055707036 *
"Handbook of Photonics for Biomedical Science", vol. 20102371, 18 May 2010, CRC PRESS, ISBN: 978-1-4398-0629-6, ISSN: 2155-2312, article BRIAN WILSON: "Photodynamic Therapy/Diagnostics : Principles, Practice, and Advances", pages: 649 - 686, XP055707033, DOI: 10.1201/9781439806296-c25 *
SETA BIOMEDICALS: "SETA BioMedicals SETA BioMedicals Fluorescent Tools for BioMedical Applications Product number: K10-7801 Product name: Seta-APC-780 General Data", 16 November 2014 (2014-11-16), XP055707118, Retrieved from the Internet <URL:https://www.setabiomedicals.com/files/Pdf/K10-7801.pdf> [retrieved on 20200619] *

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