WO2021020953A3 - Wnt 단백질 활성 증진용 배지 조성물 - Google Patents

Wnt 단백질 활성 증진용 배지 조성물 Download PDF

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WO2021020953A3
WO2021020953A3 PCT/KR2020/010229 KR2020010229W WO2021020953A3 WO 2021020953 A3 WO2021020953 A3 WO 2021020953A3 KR 2020010229 W KR2020010229 W KR 2020010229W WO 2021020953 A3 WO2021020953 A3 WO 2021020953A3
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protein activity
medium composition
wnt protein
enhancing wnt
enhancing
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PCT/KR2020/010229
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English (en)
French (fr)
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WO2021020953A2 (ko
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이진우
김철훈
권순성
여주혜
Original Assignee
연세대학교 산학협력단
주식회사 인터파크바이오컨버전스
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Priority claimed from KR1020200054946A external-priority patent/KR102414138B1/ko
Application filed by 연세대학교 산학협력단, 주식회사 인터파크바이오컨버전스 filed Critical 연세대학교 산학협력단
Priority to EP20846486.7A priority Critical patent/EP4043549A2/en
Priority to CN202080067854.5A priority patent/CN114502722A/zh
Priority to JP2022506405A priority patent/JP2022543042A/ja
Priority to US17/631,645 priority patent/US20220275344A1/en
Publication of WO2021020953A2 publication Critical patent/WO2021020953A2/ko
Publication of WO2021020953A3 publication Critical patent/WO2021020953A3/ko

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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 Wnt 단백질의 활성 증진 및 오가노이드 배양을 위한 무혈청 배지 조성물 및 이를 이용한 오가노이드 배양 방법에 관한 것이다.
PCT/KR2020/010229 2019-08-01 2020-08-03 Wnt 단백질 활성 증진용 배지 조성물 WO2021020953A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP20846486.7A EP4043549A2 (en) 2019-08-01 2020-08-03 Medium composition for enhancing wnt protein activity
CN202080067854.5A CN114502722A (zh) 2019-08-01 2020-08-03 用于增进Wnt蛋白的活性的培养基组合物
JP2022506405A JP2022543042A (ja) 2019-08-01 2020-08-03 Ｗｎｔタンパク質活性増進用の培地組成物
US17/631,645 US20220275344A1 (en) 2019-08-01 2020-08-03 Medium composition for enhancing wnt protein activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20190093754 2019-08-01
KR10-2019-0093754 2019-08-01
KR10-2020-0054946 2020-05-08
KR1020200054946A KR102414138B1 (ko) 2019-08-01 2020-05-08 Wnt 단백질 활성 증진용 배지 조성물

Publications (2)

Publication Number Publication Date
WO2021020953A2 WO2021020953A2 (ko) 2021-02-04
WO2021020953A3 true WO2021020953A3 (ko) 2021-05-20

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PCT/KR2020/010229 WO2021020953A2 (ko) 2019-08-01 2020-08-03 Wnt 단백질 활성 증진용 배지 조성물

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Country Link
US (1) US20220275344A1 (ko)
EP (1) EP4043549A2 (ko)
JP (1) JP2022543042A (ko)
CN (1) CN114502722A (ko)
WO (1) WO2021020953A2 (ko)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017199811A1 (ja) * 2016-05-18 2017-11-23 学校法人慶應義塾 オルガノイド培養用細胞培養培地、培養方法、及びオルガノイド
JP6376578B2 (ja) * 2016-02-18 2018-08-22 学校法人慶應義塾 細胞培養培地、培養方法、及びオルガノイド
KR20190040212A (ko) * 2016-08-24 2019-04-17 각고호우징 게이오기주크 인간 설사병 바이러스의 감염·증식 배양용 2d 오가노이드 및 그 사용

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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MX2014013725A (es) * 2012-05-23 2015-02-10 Hoffmann La Roche Composiciones y metodos para obtener y utilizar celulas del endodermo y hepatocitos.
US20190338374A1 (en) * 2018-04-23 2019-11-07 Keio University Klebsiella pneumoniae strain that induces inflammation in the liver
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6376578B2 (ja) * 2016-02-18 2018-08-22 学校法人慶應義塾 細胞培養培地、培養方法、及びオルガノイド
WO2017199811A1 (ja) * 2016-05-18 2017-11-23 学校法人慶應義塾 オルガノイド培養用細胞培養培地、培養方法、及びオルガノイド
KR20190040212A (ko) * 2016-08-24 2019-04-17 각고호우징 게이오기주크 인간 설사병 바이러스의 감염·증식 배양용 2d 오가노이드 및 그 사용

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EMIKO MIHARA, HIDENORI HIRAI, HIDEKI YAMAMOTO, KEIKO TAMURA-KAWAKAMI, MAMI MATANO, AKIRA KIKUCHI, TOSHIRO SATO, JUNICHI TAKAGI: "Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin", ELIFE, ELIFE SCIENCES PUBLICATIONS, vol. 5, 23 February 2016 (2016-02-23), XP055477142, DOI: 10.7554/eLife.11621 *
TÜYSÜZ NESRIN, VAN BLOOIS LOUIS, VAN DEN BRINK STIENEKE, BEGTHEL HARRY, VERSTEGEN MONIQUE M. A., CRUZ LUIS J., HUI LIJIAN, VAN DER: "Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells", NATURE COMMUNICATIONS, vol. 8, no. 14578, 1 April 2017 (2017-04-01), pages 1 - 11, XP055812689, DOI: 10.1038/ncomms14578 *

Also Published As

Publication number Publication date
JP2022543042A (ja) 2022-10-07
CN114502722A (zh) 2022-05-13
EP4043549A2 (en) 2022-08-17
US20220275344A1 (en) 2022-09-01
WO2021020953A2 (ko) 2021-02-04

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