WO2020238406A1

WO2020238406A1 - Feed composition and preparation method therefor and application thereof

Info

Publication number: WO2020238406A1
Application number: PCT/CN2020/082965
Authority: WO
Inventors: 赖州文; 龙吉云; 黄艺珠; 杨立军; 章亮
Original assignee: 山东省新奥农牧发展有限公司
Priority date: 2019-05-30
Filing date: 2020-04-02
Publication date: 2020-12-03

Abstract

A feed emulsion composition, comprising butyrate, butyrin, butyrin derivatives or a mixture thereof, and/or glycerol laurate, glycerol laurate derivatives or a mixture thereof, wherein the pH value of the feed emulsion composition is 2-6. A feed composition, a preparation method, and an application of the composition in feeding animals. The feed composition comprises butyrin and polyglycerol laurate, and the pH value is 2.5-6.0.

Description

饲用组合物及其制备方法和应用Feed composition and preparation method and application thereof

相关申请Related application

本申请要求2019年5月30提交的中国专利申请201910462502.9号以及2019年7月8日提交的中国专利申请201910609010.8号的优先权，通过引用将两篇专利申请的内容全部并入本文，用于所有目的。This application claims the priority of Chinese patent application No. 201910462502.9 filed on May 30, 2019 and Chinese patent application No. 201910609010.8 filed on July 8, 2019. The contents of the two patent applications are incorporated herein by reference for all purpose.

技术领域Technical field

本申请大体涉及动物饲料产品领域；更具体而言，本申请涉及适于动物饲养、特别是动物病防治的饲用组合物及其制备方法和应用。The present application generally relates to the field of animal feed products; more specifically, the present application relates to a feed composition suitable for animal feeding, especially animal disease control, and a preparation method and application thereof.

背景技术Background technique

畜禽饮水或饲料的酸化、霉变以及病原微生物的滋生易引起畜禽中毒，影响畜禽生长，降低畜禽机体抵抗力、甚至引发疫病的爆发，给畜牧业发展造成严重的经济损失。The acidification, mildew and growth of pathogenic microorganisms in livestock and poultry drinking water or feed can easily cause livestock and poultry poisoning, affect the growth of livestock and poultry, reduce the resistance of livestock and poultry, and even cause disease outbreaks, causing serious economic losses to the development of animal husbandry.

为了解决上述问题，研发人员进行了不断的努力和探索，研发出一些产品，但是仍然无法满足畜牧业生产的需要。因此，迫切需要开发新的、经济有效的用于畜禽饮水或饲料的防酸化、防霉变和/或防病原微生物的产品，促进畜牧业经济的可持续发展。In order to solve the above problems, R&D personnel have made continuous efforts and explorations to develop some products, but they still cannot meet the needs of animal husbandry production. Therefore, there is an urgent need to develop new, cost-effective and anti-acidification, anti-mildew, and/or anti-pathogenic microorganism products for livestock and poultry drinking water or feed to promote the sustainable economic development of animal husbandry.

此外，诸如病毒、细菌等的致病微生物感染是长期困扰动物饲养的问题，也是产业内一直面对的挑战和研究热点。John J.Kabara于1966年发现饱和脂肪酸中中链脂肪酸月桂酸(C ₁₂)的杀菌能力较强，而单不饱和脂肪酸中棕榈油酸(C _16:1)的杀菌能力更为优异。Himarsson等(2006年)进一步证实了这一点，并且发现月桂酸在酸性环境下对Visna病毒的抑制作用更强。此外，还有报道证实，基于短链脂肪酸(如丁酸)的物质能抑制革兰氏阴性细菌如沙门氏杆菌、大肠杆菌。 In addition, the infection of pathogenic microorganisms such as viruses and bacteria is a problem that has plagued animal feeding for a long time, and it is also a challenge and research hotspot that the industry has been facing. John J. Kabara discovered in 1966 that the medium-chain fatty acid lauric acid (C ₁₂ ) in the saturated fatty acid has a stronger bactericidal ability, while the palmitoleic acid (C _16:1 ) in the monounsaturated fatty acid has a better bactericidal ability. Himarsson et al. (2006) further confirmed this point and found that lauric acid has a stronger inhibitory effect on Visna virus in an acidic environment. In addition, there are reports confirming that substances based on short-chain fatty acids (such as butyric acid) can inhibit Gram-negative bacteria such as Salmonella and Escherichia coli.

基于中、短链脂肪酸的抗微生物制剂开发具有良好的前景，也是本领域亟需的。The development of antimicrobial preparations based on medium and short-chain fatty acids has good prospects and is also urgently needed in this field.

发明内容Summary of the invention

第一方面，本申请提供了饲用乳液组合物，其包含丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物，其中所述饲用乳液组合物的pH值为2-6。In the first aspect, this application provides a feed emulsion composition, which comprises butyrate, butyrate, butyrate derivatives or mixtures thereof, and/or laurate, laurate derivatives or The mixture, wherein the pH value of the feed emulsion composition is 2-6.

在第一方面的一些实施方案中，所述饲用乳液组合物的pH值为2.5-5.5。In some embodiments of the first aspect, the pH of the feed emulsion composition is 2.5-5.5.

在第一方面的一些实施方案中，所述饲用乳液组合物在用水稀释100-1000倍后的pH值为3-6，优选为3.5-5.5。In some embodiments of the first aspect, the feed emulsion composition has a pH value of 3-6, preferably 3.5-5.5 after being diluted 100-1000 times with water.

第二方面，本申请提供了制备饲用乳液组合物的方法，包括将丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物，与乳化剂、稳定剂、有机酸和甜味剂进行乳化均质处理；其中，通过所述方法制备的饲用乳液组合物的pH值为2-6。In the second aspect, the present application provides a method for preparing a feed emulsion composition, which comprises combining butyrate, butyrate, butyrate derivatives or mixtures thereof, and/or laurate, laurate Derivatives or their mixtures are emulsified and homogenized with emulsifiers, stabilizers, organic acids and sweeteners; wherein the pH value of the feed emulsion composition prepared by the method is 2-6.

在第二方面的一些实施方案中，通过第二方面所述的方法制备的饲用乳液组合物的pH值为2.5-5.5。In some embodiments of the second aspect, the feed emulsion composition prepared by the method described in the second aspect has a pH of 2.5-5.5.

在第一方面和第二方面的一些实施方案中，所述丁酸盐选自钠盐、钾盐、钙盐、镁盐以及它们的任意组合。In some embodiments of the first and second aspects, the butyrate is selected from the group consisting of sodium, potassium, calcium, magnesium, and any combination thereof.

在第一方面和第二方面的一些实施方案中，所述丁酸甘油酯选自三丁酸甘油酯、单丁酸甘油酯、双丁酸甘油酯以及它们的任意组合。In some embodiments of the first and second aspects, the glyceryl butyrate is selected from the group consisting of tributyrin, glyceryl monobutyrate, glyceryl dibutyrate, and any combination thereof.

在第一方面和第二方面的一些实施方案中，所述丁酸甘油酯衍生物选自丁酸月桂酸甘油酯，丁酸辛酸甘油酯，丁酸癸酸甘油酯，丁酸辛酸癸酸甘油酯，丁酸、辛酸、癸酸和月桂酸与甘油的组配甘油酯，以及它们的任意组合。In some embodiments of the first aspect and the second aspect, the butyric acid glyceride derivative is selected from the group consisting of glyceryl butyrate laurate, glyceryl butyrate caprylate, glyceryl butyrate caprate, and glyceryl butyrate caprylic acid. Esters, butyric acid, caprylic acid, capric acid and lauric acid and glycerin combination glycerides, and any combination thereof.

在第一方面和第二方面的一些实施方案中，所述月桂酸甘油酯选自聚甘油月桂酸酯、单月桂酸甘油酯、双月桂酸甘油酯、三月桂酸甘油酯以及它们的任意组合。In some embodiments of the first and second aspects, the glyceryl laurate is selected from polyglyceryl laurate, glyceryl monolaurate, glyceryl dilaurate, glyceryl trilaurate, and any combination thereof .

在第一方面和第二方面的一些实施方案中，所述月桂酸甘油酯衍生物选自月桂酸和其他短中长链脂肪酸与甘油的酯化物。In some embodiments of the first and second aspects, the lauric acid glyceride derivative is selected from esters of lauric acid and other short, medium and long chain fatty acids with glycerol.

在第二方面的一些实施方案中，所述乳化剂选自硬酯酰乳酸钠、复合乳化剂、双乙酰酒石酸单甘油酯、以及它们的任意组合，其中所述复合乳化剂包含甘油脂肪酸脂和蔗糖脂肪酸脂及硬脂酰乳酸钠-钙。In some embodiments of the second aspect, the emulsifier is selected from the group consisting of sodium stearoyl lactylate, a complex emulsifier, diacetyltartaric acid monoglyceride, and any combination thereof, wherein the complex emulsifier comprises glycerol fatty acid ester and sucrose Fatty acid esters and sodium stearoyl lactylate-calcium.

在第二方面的一些实施方案中，所述稳定剂选自酪蛋白、变性淀粉、卡拉胶以及它们的任意组合。In some embodiments of the second aspect, the stabilizer is selected from casein, modified starch, carrageenan, and any combination thereof.

在第二方面的一些实施方案中，所述有机酸选自丙酸、柠檬酸、乳酸以及它们的任意组合。In some embodiments of the second aspect, the organic acid is selected from propionic acid, citric acid, lactic acid, and any combination thereof.

在第二方面的一些实施方案中，所述甜味剂选自三氯蔗糖、阿斯巴甜、蔗糖以及它们的任意组合。In some embodiments of the second aspect, the sweetener is selected from sucralose, aspartame, sucrose, and any combination thereof.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物为0wt.％～80wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the butyrate, butyrin, butyrin derivatives or mixtures thereof are 0wt.% to 80wt. %.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物为0wt.％～80wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the glyceryl laurate, glyceryl laurate derivative or a mixture thereof is 0 wt.% to 80 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述乳化剂为0wt.％～20wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the emulsifier is 0 wt.% to 20 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述有机酸为0.01wt.％～10wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the organic acid is 0.01 wt.% to 10 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述甜味剂为0wt.％～5wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the sweetener is 0 wt.% to 5 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述稳定剂为0wt.％～58wt.％。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the stabilizer is 0 wt.% to 58 wt.%.

在第二方面的一些实施方案中，所述方法包括以下步骤：In some embodiments of the second aspect, the method includes the following steps:

将乳化剂溶解于丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物中，得到油相；Dissolving the emulsifier in butyric acid glyceride, a butyric acid glyceride derivative or a mixture thereof, and/or lauric acid glyceride, a lauric acid glyceride derivative or a mixture thereof to obtain an oil phase;

将稳定剂、有机酸和甜味剂溶解并混合，得到水相；Dissolve and mix the stabilizer, organic acid and sweetener to obtain an aqueous phase;

对水相和油相进行混合、乳化、均质化处理。The water phase and the oil phase are mixed, emulsified, and homogenized.

在一些实施方案中，根据第二方面所述的方法制备的饲用乳液组合物具有第一方面所述的饲用乳液组合物的性质。In some embodiments, the feed emulsion composition prepared according to the method described in the second aspect has the properties of the feed emulsion composition described in the first aspect.

在第二方面的一些实施方案中，所述乳化剂与所述稳定剂的质量比为1：(4-11)。In some embodiments of the second aspect, the mass ratio of the emulsifier to the stabilizer is 1: (4-11).

在第二方面的一些实施方案中，卡拉胶、变性淀粉、复合乳化剂与双乙酰酒石酸单甘油酯的质量比为1：(70-140)：(7-25)：(0-7)。In some embodiments of the second aspect, the mass ratio of carrageenan, modified starch, composite emulsifier and diacetyltartaric acid monoglyceride is 1: (70-140): (7-25): (0-7).

在第二方面的一些实施方案中，所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的质量比为1：(0.1-10)：(0-60)。In some embodiments of the second aspect, the mass ratio of the sweetener, the organic acid and the butyrate, butyrate, butyrate derivatives or mixtures thereof is 1: (0.1- 10): (0-60).

第三方面，本申请提供了第一方面所述的饲用乳液组合物或根据第二方面所述的方法制备的饲用乳液组合物在动物饲养中的用途。In the third aspect, this application provides the use of the feed emulsion composition of the first aspect or the feed emulsion composition prepared according to the method of the second aspect in animal feeding.

在第三方面的一些实施方案中，所述动物选自家畜类、家禽类或水产类(例如鱼类)。In some embodiments of the third aspect, the animal is selected from livestock, poultry, or aquatic products (e.g., fish).

第四方面，本申请提供了第一方面所述的饲用乳液组合物或根据第二方面所述的方法制备的饲用乳液组合物在制备用于预防或治疗诸如家畜类、家禽类或水产类的动物中的微生物感染相关病症的药物中的用途。In the fourth aspect, this application provides that the feed emulsion composition of the first aspect or the feed emulsion composition prepared according to the method of the second aspect is prepared for the prevention or treatment of livestock, poultry or aquatic products. Use in medicines for diseases related to microbial infections in animals.

在一些实施方案中，微生物感染为病毒感染或细菌感染。在一些实施方案中，所述病毒为囊膜病毒，例如非洲猪瘟病毒(ASFV)、蓝耳病病毒、伪狂犬病毒、流感病毒、流行性腹泻病毒。在一些实施方案中，所述细菌选自沙门氏杆菌、大肠杆菌、金黄色葡萄球菌、空肠弯曲杆菌、梭菌。In some embodiments, the microbial infection is a viral infection or a bacterial infection. In some embodiments, the virus is an enveloped virus, such as African swine fever virus (ASFV), PRRS virus, pseudorabies virus, influenza virus, pandemic diarrhea virus. In some embodiments, the bacteria are selected from Salmonella, Escherichia coli, Staphylococcus aureus, Campylobacter jejuni, Clostridium.

第五方面，本申请提供了饲用组合物，所述饲用组合物包含丁酸甘油酯和聚甘油月桂酸酯，并且pH值为2.5-6.0。In a fifth aspect, the present application provides a feed composition, which contains butyric acid glyceride and polyglyceryl laurate, and has a pH of 2.5-6.0.

在一些实施方案中，丁酸甘油酯选自一丁酸甘油酯、二丁酸甘油酯、三丁酸甘油酯或以上的任意组合。In some embodiments, the butyrin is selected from monobutyrin, dibutyrin, tributyrin, or any combination of the above.

在一些实施方案中，聚甘油月桂酸酯为聚甘油月桂酸甘油酯。在一些具体实施方案中，聚甘油月桂酸酯为聚甘油月桂酸单甘酯。In some embodiments, the polyglycerol laurate is polyglycerol laurate. In some specific embodiments, the polyglycerol laurate is polyglycerol monolaurate.

在一些实施方案中，饲用组合物为酸性乳液的形式，pH值为2.5-3.0。在一些实施方案中，酸性乳液包含乳化剂、稳定剂、有机酸和调味剂中的一种或多种。In some embodiments, the feed composition is in the form of an acid emulsion with a pH of 2.5-3.0. In some embodiments, the acidic emulsion includes one or more of emulsifiers, stabilizers, organic acids, and flavoring agents.

在一些实施方案中，乳化剂选自柠檬酸脂肪酸甘油酯、硬酯酰乳酸钠、硬酯酰乳酸钙、单/双脂肪酸甘油酯、以及它们的任意组合。In some embodiments, the emulsifier is selected from citric acid fatty acid glycerides, sodium stearoyl lactylate, calcium stearoyl lactate, mono/di fatty acid glycerides, and any combination thereof.

在一些实施方案中，稳定剂选自酪蛋白、变性淀粉(例如辛烯基琥珀酸淀粉钠)、卡拉胶以及它们的任意组合。In some embodiments, the stabilizer is selected from casein, modified starch (such as sodium starch octenyl succinate), carrageenan, and any combination thereof.

在一些实施方案中，有机酸选自丙酸、柠檬酸、乳酸以及它们的任意组合。In some embodiments, the organic acid is selected from propionic acid, citric acid, lactic acid, and any combination thereof.

在一些实施方案中，调味剂选自三氯蔗糖、阿斯巴甜、蔗糖以及它们的任意组合。In some embodiments, the flavoring agent is selected from sucralose, aspartame, sucrose, and any combination thereof.

在一些实施方案中，以所述饲用组合物的总重量计，丁酸甘油酯为0.1wt.％～60wt.％。In some embodiments, based on the total weight of the feed composition, the butyric acid glyceride is 0.1 wt.% to 60 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，聚甘油月桂酸酯为0.1wt.％～60wt.％。In some embodiments, based on the total weight of the feed composition, the polyglyceryl laurate is 0.1 wt.% to 60 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，乳化剂为0wt.％～20wt.％。In some embodiments, based on the total weight of the feed composition, the emulsifier is 0 wt.% to 20 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，有机酸为0.01wt.％～10wt.％。In some embodiments, the organic acid is 0.01 wt.% to 10 wt.% based on the total weight of the feed composition.

在一些实施方案中，以所述饲用组合物的总重量计，调味剂为0wt.％～5wt.％。In some embodiments, based on the total weight of the feed composition, the flavoring agent is 0 wt.% to 5 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，稳定剂为0wt.％～40wt.％。In some embodiments, based on the total weight of the feed composition, the stabilizer is 0 wt.% to 40 wt.%.

第六方面，本申请提供了制备饲用组合物的方法，所述饲用组合物为包含丁酸甘油酯和聚甘油月桂酸酯的酸性乳液的形式，并且pH值为2.5-3.0，所述方法包括：In the sixth aspect, the present application provides a method for preparing a feed composition, which is in the form of an acid emulsion containing butyric acid glyceride and polyglyceryl laurate, and has a pH of 2.5-3.0. Methods include:

将丁酸甘油酯、聚甘油月桂酸酯与乳化剂、稳定剂、有机酸和任选的调味剂进行乳化均质处理。Glyceryl butyrate, polyglyceryl laurate, emulsifier, stabilizer, organic acid and optional flavoring agent are emulsified and homogenized.

第七方面，本申请提供了丁酸甘油酯和聚甘油月桂酸酯在制备饲用组合物中的用途，其中所述饲用组合物的pH值为2.5-6.0。In the seventh aspect, the present application provides the use of glyceryl butyrate and polyglyceryl laurate in preparing a feed composition, wherein the pH of the feed composition is 2.5-6.0.

在一些实施方案中，丁酸甘油酯选自一丁酸甘油酯、二丁酸甘油酯、三丁酸甘油酯或以上的任意组合。In some embodiments, the glyceryl butyrate is selected from glyceryl monobutyrate, glyceryl dibutyrate, tributyrin, or any combination of the above.

在第五至第七方面的一些实施方案中，饲用组合物用于预防或治疗诸如家畜类、家禽类或水产类的动物中的微生物感染相关病症。在一些实施方案中，微生物感染为病毒感染或细菌感染。在一些实施方案中，所述病毒为囊膜病毒，例如非洲猪瘟病毒(ASFV)、蓝耳病病毒、伪狂犬病毒、流感病毒、流行性腹泻病毒。在一些实施方案中，所述细菌选自沙门氏杆菌、大肠杆菌、金黄色葡萄球菌、空肠弯曲杆菌、梭菌。In some embodiments of the fifth to seventh aspects, the feed composition is used to prevent or treat microbial infection-related disorders in animals such as livestock, poultry, or aquatic products. In some embodiments, the microbial infection is a viral infection or a bacterial infection. In some embodiments, the virus is an enveloped virus, such as African swine fever virus (ASFV), PRRS virus, pseudorabies virus, influenza virus, pandemic diarrhea virus. In some embodiments, the bacteria are selected from Salmonella, Escherichia coli, Staphylococcus aureus, Campylobacter jejuni, Clostridium.

第八方面，本申请提供了预防或治疗诸如家畜类或家禽类的动物中的微生物感染相关病症的方法，包括向有需要的动物给予在第五至第七方面所述的饲用组合物。In the eighth aspect, the present application provides a method for preventing or treating microbial infection-related diseases in animals such as livestock or poultry, including administering the feed composition described in the fifth to seventh aspects to animals in need.

附图简要描述Brief description of the drawings

图1显示了阴性对照组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 1 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the negative control group.

图2显示了阳性对照组的病毒荧光观察。Figure 2 shows the virus fluorescence observation of the positive control group.

图3显示了月桂酸1.4mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 3 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the lauric acid 1.4mg/ml concentration group.

图4显示了月桂酸0.7mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 4 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the 0.7 mg/ml lauric acid concentration group.

图5显示了月桂酸0.28mg/ml浓度组的病毒荧光观察。Figure 5 shows the virus fluorescence observation of the lauric acid 0.28mg/ml concentration group.

图6显示了月桂酸单甘脂1.92mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 6 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the 1.92 mg/ml concentration group of lauric acid monoglyceride.

图7显示了月桂酸单甘脂0.96mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 7 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the 0.96 mg/ml concentration of lauric acid monoglyceride.

图8显示了月桂酸单甘脂0.384mg/ml浓度组的病毒荧光观察。Figure 8 shows the fluorescence observation of viruses in the 0.384 mg/ml concentration group of lauric acid monoglyceride.

图9显示了聚甘油月桂酸单甘酯3mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 9 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the polyglycerol monolaurate 3mg/ml concentration group.

图10显示了聚甘油月桂酸单甘酯1.5mg/ml浓度组病毒荧光观察。Figure 10 shows the fluorescence observation of the virus in the polyglycerol monolaurate 1.5 mg/ml concentration group.

图11显示了聚甘油月桂酸单甘酯0.6mg/ml浓度组病毒荧光观察。Figure 11 shows the fluorescence observation of the virus in the polyglycerol monolaurate 0.6 mg/ml concentration group.

图12显示了酸性乳液40mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 12 shows the cell morphology observation (top) and virus fluorescence observation (bottom) of the acid emulsion 40mg/ml concentration group.

图13显示了酸性乳液100mg/ml浓度组的细胞形态学观察(上)和病毒荧光观察(下)。Figure 13 shows cell morphology observation (top) and virus fluorescence observation (bottom) of the acid emulsion 100mg/ml concentration group.

图14显示了使用酸性乳液之前(上)和之后(下)的禽粪便的图片。Figure 14 shows pictures of poultry manure before (top) and after (bottom) use of the acid emulsion.

发明详细描述Detailed description of the invention

本申请的发明人对含有丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物的饲用乳液组合物制备进行了研究。丁酸盐、三丁酸甘油酯及其衍生物可以在消化道内释放出丁酸，修复受损的肠道粘膜，增强消化道的屏障功能，月桂酸甘油酯及其衍生物具有抵抗病源微生物感染的能力，对潜在的病原微生物具有预防作用，低pH值有利于抑制畜禽饮水或饲料的霉变和病原微生物的滋生，如果能够使得含有丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物的饲用乳液组合物在低pH值(例如pH值为2-6)下保持稳定，就能够为畜禽提供良好的饮水或饲料天然抗病毒、抗致病菌作用。相比之下，常规的脂肪酸酯乳状液在低pH条件下不稳定，而且在用水稀释100-1000倍后，不能维持低pH值。此外，尽管丁酸盐及其衍生物具有诸多生物学益处，丁酸盐及其衍生物通常具有差的气味及口感，将其直接添加至饲料中会带来适口性问题。针对上述技术问题，本申请的发明人经过研究建立了本申请的各项发明，本申请的各项发明能够解决至少一个上述技术问题，并且在一些情况下还具有其他优势。The inventor of the present application prepared a feed emulsion composition containing butyrate, butyrate, butyrate derivatives or mixtures thereof, and/or lauric acid glycerides, laurate glycerides derivatives or mixtures thereof Were studied. Butyrate, tributyrin and its derivatives can release butyric acid in the digestive tract, repair damaged intestinal mucosa, and enhance the barrier function of the digestive tract. Glycerol laurate and its derivatives can resist pathogenic microbial infections It has a preventive effect on potential pathogenic microorganisms. The low pH value is beneficial to inhibit the mildew of livestock and poultry drinking water or feed and the growth of pathogenic microorganisms. If it can make it contain butyrate, butyrin and butyrin The feed emulsion composition of glyceryl laurate, lauric glyceride derivatives or mixtures thereof is stable at a low pH value (for example, a pH value of 2-6), and can be used for livestock and poultry. Provide good drinking water or feed with natural anti-virus and anti-pathogenic effects. In contrast, conventional fatty acid ester emulsions are unstable under low pH conditions and cannot maintain low pH values after being diluted 100-1000 times with water. In addition, although butyrate and its derivatives have many biological benefits, butyrate and its derivatives usually have poor odor and taste, and adding them directly to feed will cause palatability problems. In view of the above technical problems, the inventor of this application has established various inventions of this application through research, and each invention of this application can solve at least one of the above technical problems, and in some cases also has other advantages.

此外，本申请的发明人基于脂肪酸、特别是基于丁酸(短链脂肪酸)和月桂酸(中链脂肪酸)的化合物在动物抗病方面进行了深入研究，发现丁酸甘油酯和聚甘油月桂酸酯联合使用具有优异的效果。此外，鉴于月桂酸及其酯类通常呈固态，不适合动物饮水添加或直接灌服，本申请的发明人还进一步开发出更方便给予动物的饲用组合物制剂，例如，以酸性乳液的形式。由此，建立了本申请的各项发明。In addition, the inventors of the present application have conducted in-depth research on animal disease resistance based on fatty acids, especially compounds based on butyric acid (short-chain fatty acid) and lauric acid (medium-chain fatty acid), and found that butyric acid glycerides and polyglyceryl lauric acid The combination of esters has excellent effects. In addition, in view of the fact that lauric acid and its esters are usually solid and are not suitable for animal drinking water addition or direct administration, the inventor of the present application has further developed a feed composition formulation that is more convenient to administer to animals, for example, in the form of an acid emulsion . Thus, various inventions of this application have been established.

定义definition

提供以下定义用以更好地界定本申请以及在本申请实践中指导本领域普通技术人员。除非另外说明，本申请中的术语的含义与本领域技术人员通常理解的含义相同，例如，涉及原料和产物、操作步骤、工艺参数、使用设备和工具以及数值单位中的术语。本文所引用的所有专利文献、学术论文及其他公开出版物，其中的全部内容整体并入本文作为参考。The following definitions are provided to better define this application and to guide those of ordinary skill in the art in the practice of this application. Unless otherwise specified, the meanings of the terms in this application are the same as those commonly understood by those skilled in the art, for example, terms related to raw materials and products, operating steps, process parameters, equipment and tools used, and numerical units. All patent documents, academic papers and other public publications cited in this article, all of which are incorporated herein by reference in their entirety.

本文所用的术语“丁酸甘油酯衍生物”指适于给予动物的结构源于丁酸甘油酯的物质。例如，常见的丁酸甘油酯衍生物为丁酸月桂酸甘油酯，丁酸辛酸甘油酯，丁酸癸酸甘油酯，丁酸辛酸癸酸甘油酯，丁酸、辛酸、癸酸和月桂酸与甘油的组配甘油酯等。The term "butyric acid derivative" as used herein refers to a substance suitable for administration to animals whose structure is derived from butyric acid glyceride. For example, common butyric acid glyceride derivatives are butyric acid lauric acid glyceride, butyric acid caprylic acid glyceride, butyric acid capric glyceride, butyric acid caprylic acid capric glyceride, butyric acid, caprylic acid, capric acid and lauric acid and Glycerin composition glyceride etc.

本文所用的术语“丁酸盐”指适于给予动物的丁酸的盐。例如，合适的丁酸盐包括钠盐、钾盐、钙盐、镁盐等。The term "butyrate" as used herein refers to a salt of butyric acid suitable for administration to animals. For example, suitable butyrates include sodium, potassium, calcium, magnesium, and the like.

本文所用的术语“月桂酸甘油酯衍生物”指适于给予动物的结构源于月桂酸甘油酯的物质。例如，常见的月桂酸甘油酯衍生物为月桂酸和其他短中长链脂肪酸与甘油的酯化物等。The term "glyceryl laurate derivative" as used herein refers to a substance suitable for administration to animals whose structure is derived from glyceryl laurate. For example, common lauric acid glyceride derivatives are esters of lauric acid and other short, medium and long chain fatty acids with glycerol.

本文所用的术语“乳化剂”是指能够改善乳浊液中各种构成相之间的表面张力，使之形成均匀稳定的分散体系或乳浊液的物质。例如，合适的乳化剂包括但不限于硬酯酰乳酸钠、复合乳化剂、双乙酰酒石酸单甘油酯等。As used herein, the term "emulsifier" refers to a substance that can improve the surface tension between the various constituent phases in the emulsion to form a uniform and stable dispersion system or emulsion. For example, suitable emulsifiers include but are not limited to sodium stearoyl lactylate, complex emulsifiers, diacetyl tartaric acid monoglyceride and the like.

本文所用的术语“复合乳化剂”是指一种或几种乳化剂的组合。例如复合乳化剂可以包含甘油脂肪酸脂和蔗糖脂肪酸脂及硬脂酰乳酸钠-钙。The term "complex emulsifier" as used herein refers to a combination of one or several emulsifiers. For example, the complex emulsifier may include glycerol fatty acid ester, sucrose fatty acid ester, and sodium stearoyl lactylate-calcium.

本文所用的术语“稳定剂”是指能增加本申请的脂肪酸酯乳液的稳定性的化学物质。例如，合适的稳定剂包括但不限于酪蛋白、变性淀粉、卡拉胶等。The term "stabilizer" as used herein refers to a chemical substance that can increase the stability of the fatty acid ester emulsion of the present application. For example, suitable stabilizers include but are not limited to casein, modified starch, carrageenan and the like.

本文所用的术语“乳液包材”，也称作“乳液壁材”，是指能使乳状液稳定存在的除乳化剂以外的其他材质。例如，合适的乳液包材包括但不限于食品胶体、糊精、糖浆、壳聚糖、乳糖、乳清粉等。As used herein, the term "emulsion packaging material", also known as "emulsion wall material", refers to materials other than emulsifiers that can stabilize the emulsion. For example, suitable emulsion packaging materials include, but are not limited to, food colloids, dextrin, syrup, chitosan, lactose, whey powder and the like.

本文所用的术语“有机酸”是指一些具有酸性的有机化合物，其可与醇反应生成酯。例如，合适的有机酸包括但不限于丙酸、柠檬酸、乳酸等。The term "organic acid" as used herein refers to some acidic organic compounds that can react with alcohols to form esters. For example, suitable organic acids include, but are not limited to, propionic acid, citric acid, lactic acid, and the like.

本文所用的术语“调味剂”是指能够赋予本申请的饲用组合物在味觉上更易被动物接受的添加剂，通常为甜味剂。The term "flavoring agent" as used herein refers to an additive that can give the feed composition of the present application a taste that is more acceptable to animals, usually a sweetener.

本文所用的术语“甜味剂”是指能够赋予本申请的脂肪酸组合物乳液甜味的添加剂。例如，合适的甜味剂包括但不限于三氯蔗糖、阿斯巴甜、蔗糖等。The term "sweetener" as used herein refers to an additive capable of imparting sweetness to the fatty acid composition emulsion of the present application. For example, suitable sweeteners include, but are not limited to, sucralose, aspartame, sucrose, and the like.

在不存在明显冲突的情况下，本文描述的数值范围当存在端点0值时，表示大于0。In the absence of an obvious conflict, the numerical range described in this article is greater than 0 when there is an endpoint 0 value.

除非另外说明，本申请中用的各种百分比含量(X％)以及成分之间的比值(X：Y)是基于重量/重量。Unless otherwise specified, various percentages (X%) and ratios (X:Y) between ingredients used in this application are based on weight/weight.

应当理解，本文所用的术语“基本”、“约”(例如，在组分含量和反应参数中)以本领域技术人员通常能够理解的含义来解释。一般情况下，术语“基本”、“约”可以理解为给定数值的正负5％范围内的任意数值，例如，约X或基本X可以代表95％X至105％X的范围中的任意数值。It should be understood that the terms "substantially" and "about" (for example, in component content and reaction parameters) used herein are interpreted in the meaning that those skilled in the art can generally understand. In general, the terms "substantially" and "about" can be understood as any value within the range of plus or minus 5% of a given value. For example, about X or substantially X can represent any value in the range of 95% X to 105% X Numerical value.

还应当理解，本文中给出的具体数值(例如，在配比、温度和时间中)不仅可作为单独的数值理解，还应当认为提供了某一范围的端点值，并且可以相互组合提供其他范围。It should also be understood that the specific numerical values (for example, in the ratio, temperature, and time) given herein can be understood not only as individual numerical values, but also should be considered to provide a certain range of endpoint values, and can be combined with each other to provide other ranges .

具体实施方案Specific implementation plan

第一方面，本申请提供了饲用乳液组合物，其包含丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物。In the first aspect, this application provides a feed emulsion composition, which comprises butyrate, butyrate, butyrate derivatives or mixtures thereof, and/or laurate, laurate derivatives or Its mixture.

在第一方面的一些实施方案中，所述饲用乳液组合物的pH值为2-6，例如2、2.5、3、3.1、3.2、3.3、3.4、3.5、3.6、3.7、3.8、3.9、4、4.1、4.2、4.3、4.4、4.5、4.6、4.7、4.8、4.9、5、5.1、5.2、5.3、5.4、5.5、5.6、5.7、5.8、5.9、6，或以上任意两个值组成的范围。In some embodiments of the first aspect, the pH value of the feed emulsion composition is 2-6, such as 2, 2.5, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, or any two of the above values range.

在第一方面的一些实施方案中，所述饲用乳液组合物的pH值为2.5-5.5，例如2.5、3、3.1、3.2、3.3、3.4、3.5、3.6、3.7、3.8、3.9、4、4.1、4.2、4.3、4.4、4.5、4.6、4.7、4.8、4.9、5、5.1、5.2、5.3、5.4、5.5，或以上任意两个值组成的范围。In some embodiments of the first aspect, the pH of the feed emulsion composition is 2.5-5.5, such as 2.5, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4. 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, or any two of the above ranges.

在第一方面的一些具体的实施方案中，所述饲用乳液组合物的pH值为3.65、3.86或4.04。In some specific embodiments of the first aspect, the pH of the feed emulsion composition is 3.65, 3.86, or 4.04.

在第一方面的一些实施方案中，所述饲用乳液组合物在用水稀释100-1000 倍后的pH值为3-6，例如3、3.1、3.2、3.3、3.4、3.5、3.6、3.7、3.8、3.9、4、4.1、4.2、4.3、4.4、4.5、4.6、4.7、4.8、4.9、5、5.1、5.2、5.3、5.4、5.5、5.6、5.7、5.8、5.9、6，或以上任意两个值组成的范围。In some embodiments of the first aspect, the feed emulsion composition has a pH of 3-6 after being diluted 100-1000 times with water, such as 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, or any two of the above A range of values.

在第一方面的一些具体的实施方案中，所述饲用乳液组合物在用水稀释100倍后的pH值为3.92、4.57或4.65。In some specific embodiments of the first aspect, the feed emulsion composition has a pH of 3.92, 4.57 or 4.65 after being diluted 100 times with water.

在第一方面的一些具体的实施方案中，所述饲用乳液组合物在用水稀释500倍后的pH值为3.98、4.65或4.76。In some specific embodiments of the first aspect, the feed emulsion composition has a pH of 3.98, 4.65 or 4.76 after being diluted 500 times with water.

在第一方面的一些具体的实施方案中，所述饲用乳液组合物在用水稀释1000倍后的pH值为4.23、4.98或5.32。In some specific embodiments of the first aspect, the feed emulsion composition has a pH of 4.23, 4.98 or 5.32 after being diluted 1000 times with water.

第二方面，本申请提供了制备饲用乳液组合物的方法，包括将丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物，与乳化剂、稳定剂、有机酸和甜味剂进行乳化均质处理。In the second aspect, the present application provides a method for preparing a feed emulsion composition, which comprises combining butyrate, butyrate, butyrate derivatives or mixtures thereof, and/or laurate, laurate Derivatives or their mixtures are emulsified and homogenized with emulsifiers, stabilizers, organic acids and sweeteners.

在第二方面的一些实施方案中，通过第二方面所述方法制备的饲用乳液组合物的pH值为2-6，例如2、2.5、3、3.1、3.2、3.3、3.4、3.5、3.6、3.7、3.8、3.9、4、4.1、4.2、4.3、4.4、4.5、4.6、4.7、4.8、4.9、5、5.1、5.2、5.3、5.4、5.5、5.6、5.7、5.8、5.9、6，或以上任意两个值组成的范围。In some embodiments of the second aspect, the feed emulsion composition prepared by the method of the second aspect has a pH of 2-6, such as 2, 2.5, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6 , 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, or The range of any two values above.

在第二方面的一些实施方案中，通过第二方面所述方法制备的饲用乳液组合物的pH值为2.5-5.5，例如2.5、3、3.1、3.2、3.3、3.4、3.5、3.6、3.7、3.8、3.9、4、4.1、4.2、4.3、4.4、4.5、4.6、4.7、4.8、4.9、5、5.1、5.2、5.3、5.4、5.5，或以上任意两个值组成的范围。In some embodiments of the second aspect, the feed emulsion composition prepared by the method of the second aspect has a pH of 2.5-5.5, such as 2.5, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7 , 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, or any two of the above ranges.

在第二方面的一些具体的实施方案中，通过第二方面所述方法制备的饲用乳液组合物的pH值为3.65、3.86或4.04。In some specific embodiments of the second aspect, the feed emulsion composition prepared by the method described in the second aspect has a pH of 3.65, 3.86 or 4.04.

在第一方面和第二方面的一些实施方案中，所述丁酸甘油酯选自三丁酸甘油酯、单丁酸甘油酯、双丁酸甘油酯以及它们的任意组合。In some embodiments of the first and second aspects, the butyrin is selected from the group consisting of tributyrin, glyceryl monobutyrate, glyceryl dibutyrate and any combination thereof.

在第二方面的一些实施方案中，所述有机酸选自丙酸、柠檬酸、乳酸、以及它们的任意组合。In some embodiments of the second aspect, the organic acid is selected from propionic acid, citric acid, lactic acid, and any combination thereof.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物为0wt.％～80wt.％，例如0wt.％、 5wt.％、10wt.％、15wt.％、20wt.％、25wt.％、30wt.％、35wt.％、40wt.％、45wt.％、50wt.％、55wt.％、60wt.％、65wt.％、70wt.％、75wt.％、80wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the butyrate, butyrin, butyrin derivatives or mixtures thereof are 0wt.% to 80wt. %, for example, 0wt.%, 5wt.%, 10wt.%, 15wt.%, 20wt.%, 25wt.%, 30wt.%, 35wt.%, 40wt.%, 45wt.%, 50wt.%, 55wt.% , 60wt.%, 65wt.%, 70wt.%, 75wt.%, 80wt.%, or a range of any two of the above values.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物为0wt.％、20wt.％或25wt.％。In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, the butyrate, butyrin, butyrin derivatives or mixtures thereof are 0 wt.%, 20wt.% or 25wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物为0wt.％～80wt.％，例如0wt.％、5wt.％、10wt.％、15wt.％、20wt.％、25wt.％、30wt.％、35wt.％、40wt.％、45wt.％、50wt.％、55wt.％、60wt.％、65wt.％、70wt.％、75wt.％、80wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the lauric acid glyceride, lauric acid glyceride derivative or a mixture thereof is 0 wt.% to 80 wt.%, such as 0 wt. %, 5wt.%, 10wt.%, 15wt.%, 20wt.%, 25wt.%, 30wt.%, 35wt.%, 40wt.%, 45wt.%, 50wt.%, 55wt.%, 60wt.% , 65wt.%, 70wt.%, 75wt.%, 80wt.%, or any two of the above ranges.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，所述月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物为10wt.％、15wt.％或30wt.％。In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, the lauric acid glyceride, lauric acid glyceride derivative or a mixture thereof is 10wt.%, 15wt.% or 30wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述乳化剂为0wt.％～20wt.％，例如0wt.％、1wt.％、1.5wt.％、2wt.％、2.5wt.％、3wt.％、3.5wt.％、4wt.％、4.5wt.％、5wt.％、10wt.％、15wt.％、20wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the emulsifier is 0wt.%-20wt.%, such as 0wt.%, 1wt.%, 1.5wt.%, 2wt.%, 2.5wt.%, 3wt.%, 3.5wt.%, 4wt.%, 4.5wt.%, 5wt.%, 10wt.%, 15wt.%, 20wt.%, or any two of the above values Range.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述乳化剂为2wt.％、3wt.％或3.5wt.％。In some embodiments of the second aspect, the emulsifier is 2 wt.%, 3 wt.%, or 3.5 wt.% based on the total weight of the feed emulsion composition.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述有机酸为0.01wt.％～10wt.％，例如0.01wt.％、0.1wt.％、0.5wt.％、1wt.％、1.5wt.％、2wt.％、2.5wt.％、3wt.％、3.5wt.％、4wt.％、4.5wt.％、5wt.％、5.5wt.％、6wt.％、6.5wt.％、7wt.％、7.5wt.％、8wt.％、8.5wt.％、9wt.％、9.5wt.％、10wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the organic acid is 0.01 wt.% to 10 wt.%, such as 0.01 wt.%, 0.1 wt.%, 0.5 wt. .%, 1wt.%, 1.5wt.%, 2wt.%, 2.5wt.%, 3wt.%, 3.5wt.%, 4wt.%, 4.5wt.%, 5wt.%, 5.5wt.%, 6wt. %, 6.5wt.%, 7wt.%, 7.5wt.%, 8wt.%, 8.5wt.%, 9wt.%, 9.5wt.%, 10wt.%, or a range composed of any two of the above values.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，所述有机酸为2wt.％、3wt.％或4wt.％.In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, the organic acid is 2wt.%, 3wt.% or 4wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述甜味剂为0wt.％～5wt.％，例如0wt.％、0.5wt.％、1wt.％、1.5wt.％、2wt.％、2.5wt.％、3wt.％、3.5wt.％、4wt.％、4.5wt.％、5wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the sweetener is 0 wt.% to 5 wt.%, for example, 0 wt.%, 0.5 wt.%, 1 wt.% , 1.5wt.%, 2wt.%, 2.5wt.%, 3wt.%, 3.5wt.%, 4wt.%, 4.5wt.%, 5wt.%, or a range composed of any two of the above values.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，所述甜味剂为0.5wt.％或5wt.％。In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, the sweetener is 0.5 wt.% or 5 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述稳定剂为0wt.％～58wt.％，例如0wt.％、5wt.％、10wt.％、10.1wt.％、10.2wt.％、10.3wt.％、10.4wt.％、10.5wt.％、10.6wt.％、10.7wt.％、10.8wt.％、10.9wt.％、11wt.％、12wt.％、13wt.％、14wt.％、15wt.％、15.1wt.％、15.2wt.％、15.3wt.％、15.4wt.％、15.5wt.％、15.6wt.％、15.7wt.％、15.8wt.％、15.9wt.％、16wt.％、17wt.％、18wt.％、19wt.％、20wt.％，20.1wt.％、20.2wt.％、20.3wt.％、20.4wt.％、20.5wt.％、20.6wt.％、20.7wt.％、20.8wt.％、20.9wt.％、21wt.％、25wt.％、30wt.％、35wt.％、40wt.％、45wt.％、50wt.％、55wt.％、58wt.％、或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the stabilizer is 0 wt.% to 58 wt.%, such as 0 wt.%, 5 wt.%, 10 wt.%, 10.1 wt.%, 10.2wt.%, 10.3wt.%, 10.4wt.%, 10.5wt.%, 10.6wt.%, 10.7wt.%, 10.8wt.%, 10.9wt.%, 11wt.%, 12wt. %, 13wt.%, 14wt.%, 15wt.%, 15.1wt.%, 15.2wt.%, 15.3wt.%, 15.4wt.%, 15.5wt.%, 15.6wt.%, 15.7wt.%, 15.8 wt.%, 15.9wt.%, 16wt.%, 17wt.%, 18wt.%, 19wt.%, 20wt.%, 20.1wt.%, 20.2wt.%, 20.3wt.%, 20.4wt.%, 20.5 wt.%, 20.6wt.%, 20.7wt.%, 20.8wt.%, 20.9wt.%, 21wt.%, 25wt.%, 30wt.%, 35wt.%, 40wt.%, 45wt.%, 50wt. %, 55wt.%, 58wt.%, or any two of the above ranges.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，所述稳定剂为10.45wt.％、15.2wt.％或20.15wt.％。In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, the stabilizer is 10.45 wt.%, 15.2 wt.%, or 20.15 wt.%.

在第二方面的一些实施方案中，所述饲用乳液组合物还包含水。In some embodiments of the second aspect, the feed emulsion composition further comprises water.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，水为20 wt.％～60wt.％，例如20wt.％、25wt.％、30wt.％、35wt.％、40wt.％、45wt.％、50wt.％、55wt.％、60wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the water is 20 wt.% to 60 wt.%, such as 20 wt.%, 25 wt.%, 30 wt.%, 35 wt.% , 40wt.%, 45wt.%, 50wt.%, 55wt.%, 60wt.%, or any two of the above ranges.

在第二方面的一些具体的实施方案中，以所述饲用乳液组合物的总重量计，水为33.35wt.％、46.8wt.％或50.55wt.％。In some specific embodiments of the second aspect, based on the total weight of the feed emulsion composition, water is 33.35 wt.%, 46.8 wt.%, or 50.55 wt.%.

在第二方面的一些实施方案中，以所述饲用乳液组合物的总重量计，所述饲用乳液组合物中油脂的总含量为5wt.％～80wt.％，例如5wt.％、10wt.％、15wt.％、20wt.％、25wt.％、30wt.％、35wt.％、40wt.％、45wt.％、50wt.％、55wt.％、60wt.％、65wt.％、70wt.％、75wt.％、80wt.％，或以上任意两个值组成的范围。In some embodiments of the second aspect, based on the total weight of the feed emulsion composition, the total content of fat in the feed emulsion composition is 5 wt.% to 80 wt.%, such as 5 wt.%, 10 wt. %, 15wt.%, 20wt.%, 25wt.%, 30wt.%, 35wt.%, 40wt.%, 45wt.%, 50wt.%, 55wt.%, 60wt.%, 65wt.%, 70wt.% , 75wt.%, 80wt.%, or any two of the above ranges.

在一些实施方案中，方法包括以下步骤：In some embodiments, the method includes the following steps:

(a)将乳化剂溶解于丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物中，得到油相；(a) dissolving the emulsifier in butyric acid glyceride, a butyric acid glyceride derivative or a mixture thereof, and/or lauric acid glyceride, a lauric acid glyceride derivative or a mixture thereof, to obtain an oil phase;

(b)将稳定剂、有机酸和甜味剂溶解并混合，得到水相；(b) Dissolving and mixing stabilizers, organic acids and sweeteners to obtain an aqueous phase;

(c)对水相和油相进行混合、乳化、均质化处理。(c) The water phase and the oil phase are mixed, emulsified, and homogenized.

在一些实施方案中，步骤(b)中稳定剂(例如变性淀粉或酪蛋白)溶解于水中后被加热至50℃～90℃，例如，50℃、55℃、60℃、65℃、70℃、75℃、80℃、85℃、90℃，或以上任意两个值组成的范围。In some embodiments, in step (b), the stabilizer (for example, modified starch or casein) is dissolved in water and heated to 50°C to 90°C, for example, 50°C, 55°C, 60°C, 65°C, 70°C , 75°C, 80°C, 85°C, 90°C, or any two of the above ranges.

在一些实施方案中，乳化处理为胶体磨研或剪切泵剪切。In some embodiments, the emulsification treatment is colloidal grinding or shear pump shearing.

在一些实施方案中，均质化处理在高压下进行，例如1-70MPa，例如1MPa、5MPa、10MPa、15MPa、20MPa、25MPa、30MPa、35MPa、40MPa、45MPa、50MPa、55MPa、60MPa、65MPa、70MPa，或以上任意两个值组成的范围。In some embodiments, the homogenization treatment is performed under high pressure, such as 1-70 MPa, such as 1 MPa, 5 MPa, 10 MPa, 15 MPa, 20 MPa, 25 MPa, 30 MPa, 35 MPa, 40 MPa, 45 MPa, 50 MPa, 55 MPa, 60 MPa, 65 MPa, 70 MPa , Or the range of any two of the above values.

作为非限制性实例，油脂组合物的制备方法可以按照以下方法实施：As a non-limiting example, the preparation method of the grease composition can be implemented as follows:

按配方比例将复合乳化剂、硬酯酰乳酸钠溶于丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物中。将变性淀粉或酪蛋白或其组合物溶解于水并加热到50-90℃左右，其他稳定剂(例如卡拉胶)、有机酸、甜味剂材料溶解后，再一起混合搅拌均匀。将水相与油相混合，并经剪切乳化，控制均质压力1-70MPa将料液进行高压均质，灌装即成为产品酸乳。The compound emulsifier and sodium stearyl lactylate are dissolved in glyceryl butyrate, glyceryl butyrate derivatives or mixtures thereof, and/or glyceryl laurate, glyceryl laurate derivatives or mixtures thereof according to the formula ratio. Dissolve the modified starch or casein or their composition in water and heat to about 50-90°C. After other stabilizers (such as carrageenan), organic acids, and sweetener materials are dissolved, they are mixed together and stirred uniformly. The water phase is mixed with the oil phase, and sheared and emulsified, and the homogenization pressure is controlled at 1-70MPa to homogenize the material liquid at high pressure, and the filling becomes the product yogurt.

根据本申请方法制备的酸乳克服了乳液在酸性条件下不稳定的缺点，能够长时间保持稳定，有利于储存和运输。本申请的酸乳pH值为2-6，即使将原液稀释100-1000倍，稀释液仍可以保持pH值为3-6，添加至畜禽饮水和饲料中能够抑制微生物的生长，有助于畜禽防病防疫。The yogurt prepared according to the method of the present application overcomes the disadvantage of the emulsion being unstable under acidic conditions, can maintain stability for a long time, and is beneficial to storage and transportation. The pH value of the yogurt in this application is 2-6. Even if the original solution is diluted 100-1000 times, the diluted solution can still maintain the pH value of 3-6. Adding to livestock and poultry drinking water and feed can inhibit the growth of microorganisms and help Disease prevention and epidemic prevention of livestock and poultry.

此外根据本申请方法制备的酸乳中添加了有机酸和甜味剂，改善了口味，有良好的适口性，适用于动物食用。In addition, organic acids and sweeteners are added to the yogurt prepared according to the method of the present application, which improves the taste, has good palatability, and is suitable for animal consumption.

在第二方面的一些具体的实施方案中，所述乳化剂与所述稳定剂的质量比为1：5.05、1：6.72、1：10.13。In some specific embodiments of the second aspect, the mass ratio of the emulsifier to the stabilizer is 1:5.05, 1:6.72, 1:10.13.

在第二方面的一些实施方案中，卡拉胶、变性淀粉、复合乳化剂与双乙酰酒石酸单甘油酯的质量比为1：75：7.5：0。In some embodiments of the second aspect, the mass ratio of carrageenan, modified starch, composite emulsifier and diacetyl tartaric acid monoglyceride is 1:75:7.5:0.

在第二方面的一些实施方案中，卡拉胶、变性淀粉、复合乳化剂与双乙酰酒石酸单甘油酯的质量比为1：133.33：13.33：6.67。In some embodiments of the second aspect, the mass ratio of carrageenan, modified starch, composite emulsifier and diacetyl tartrate monoglyceride is 1:133.33:13.33:6.67.

在第二方面的一些实施方案中，卡拉胶、变性淀粉、复合乳化剂与双乙酰酒石酸单甘油酯的质量比为1：100：20：0。In some embodiments of the second aspect, the mass ratio of carrageenan, modified starch, composite emulsifier and diacetyl tartrate monoglyceride is 1:100:20:0.

卡拉胶、变性淀粉、复合乳化剂和双乙酰酒石酸单甘油酯的上述质量比是形成低pH值的稳定乳状液的关键。The above-mentioned mass ratio of carrageenan, modified starch, composite emulsifier and diacetyl tartrate monoglyceride is the key to forming a stable emulsion with low pH value.

在第二方面的一些具体的实施方案中，所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的质量比为1：8：40。In some specific embodiments of the second aspect, the mass ratio of the sweetener, the organic acid and the butyrate, butyrin, butyrin derivatives or mixtures thereof is 1:8 : 40.

在第二方面的一些具体的实施方案中，所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的质量比为1：6：50。In some specific embodiments of the second aspect, the mass ratio of the sweetener, the organic acid and the butyrate, butyrate, butyrate derivatives or mixtures thereof is 1:6 : 50.

在第二方面的一些具体的实施方案中，所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的质量比为1：0.4：0。In some specific embodiments of the second aspect, the mass ratio of the sweetener, the organic acid and the butyrate, butyrin, butyrin derivatives or mixtures thereof is 1:0.4 : 0.

所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的上述质量比在低PH值条件下将本申请的饲用乳液组合物调为甘甜，具备良好的适口性。The above-mentioned mass ratio of the sweetener, the organic acid and the butyrate, butyric acid glyceride, butyric acid glyceride derivatives or mixtures thereof is used to combine the feed emulsion composition of the present application under the condition of low pH value It is sweet and has good palatability.

适于给予本申请的饲用乳液组合物的动物包括但不限于家畜类、家禽类或水产类(例如鱼类)。Animals suitable for administering the feed emulsion composition of the present application include, but are not limited to, livestock, poultry, or aquatic products (such as fish).

本申请的饲用乳液组合物能够直接添加在水中给动物饮用或者直接与饲料拌匀食用。The feed emulsion composition of the present application can be directly added to water for animals to drink or directly mixed with feed for consumption.

第五方面，本申请提供了饲用组合物，所述饲用组合物包含丁酸甘油酯和聚甘油月桂酸酯，并且pH值为2.5-6.0(例如，2.5、3、3.5、4、4.5、5、5.5、6)。In the fifth aspect, the present application provides a feed composition, which contains butyric acid glyceride and polyglyceryl laurate, and has a pH of 2.5-6.0 (for example, 2.5, 3, 3.5, 4, 4.5 , 5, 5.5, 6).

在一些实施方案中，饲用组合物为酸性乳液的形式，pH值为2.5-3.0。在相关实施方案中，酸性乳液形式的饲用组合物适于直接添加至动物饮用水中，并且在适当的稀释比例下(例如数十倍至数百倍)，稀释液仍保持酸性，例如pH值为2.5-6.0(例如，2.5、3、3.5、4、4.5、5、5.5、6)。In some embodiments, the feed composition is in the form of an acid emulsion with a pH of 2.5-3.0. In a related embodiment, the feed composition in the form of an acid emulsion is suitable for direct addition to animal drinking water, and at an appropriate dilution ratio (for example, tens to hundreds of times), the dilution still remains acidic, such as pH The value is 2.5-6.0 (for example, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6).

在一些实施方案中，酸性乳液包含乳化剂、稳定剂、有机酸和调味剂中的一种或多种。乳化剂、稳定剂、有机酸和调味剂是乳液制备中常见类型的物质。In some embodiments, the acidic emulsion includes one or more of emulsifiers, stabilizers, organic acids, and flavoring agents. Emulsifiers, stabilizers, organic acids and flavoring agents are common types of substances in emulsion preparation.

作为非限制性的实例，合适的乳化剂包括但不限于柠檬酸脂肪酸甘油酯、硬酯酰乳酸钠、硬酯酰乳酸钙、单/双脂肪酸甘油酯、以及它们的任意组合。合适的稳定剂包括但不限于酪蛋白、变性淀粉(例如辛烯基琥珀酸淀粉钠)、卡拉胶以及它们的任意组合。合适的有机酸包括但不限于丙酸、柠檬酸、乳酸以及它们的任意组合。合适的调味剂包括但不限于三氯蔗糖、阿斯巴甜、蔗糖以及它们的任意组合。As non-limiting examples, suitable emulsifiers include, but are not limited to, citric acid fatty acid glycerides, sodium stearoyl lactylate, calcium stearoyl lactate, mono/di fatty acid glycerides, and any combination thereof. Suitable stabilizers include, but are not limited to, casein, modified starch (such as sodium starch octenyl succinate), carrageenan, and any combination thereof. Suitable organic acids include, but are not limited to, propionic acid, citric acid, lactic acid, and any combination thereof. Suitable flavoring agents include, but are not limited to, sucralose, aspartame, sucrose, and any combination thereof.

在一些实施方案中，以所述饲用组合物的总重量计，丁酸甘油酯为0.1wt.％～60wt.％(例如，0.1、0.5、1、5、10、15、20、25、30、35、40、45、50、55、60wt.％)。在一些实施方案中，以所述饲用组合物的总重量计，丁酸甘油酯为1wt.％～30wt.％。在一些实施方案中，以所述饲用组合物的总重量计，丁酸甘油酯为10wt.％～20wt.％。In some embodiments, based on the total weight of the feed composition, butyric acid glycerides are 0.1 wt.% to 60 wt.% (e.g., 0.1, 0.5, 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60wt.%). In some embodiments, based on the total weight of the feed composition, the butyric acid glyceride is 1 wt.% to 30 wt.%. In some embodiments, based on the total weight of the feed composition, the butyric acid glyceride is 10 wt.% to 20 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，聚甘油月桂酸酯为0.1wt.％～60wt.％(例如，0.1、0.5、1、5、10、15、20、25、30、35、40、45、50、55、60wt.％)。在一些实施方案中，以所述饲用组合物的总重量计，聚甘油月桂酸酯为1wt.％～30wt.％。在一些实施方案中，以所述饲用组合物的总重量计，聚甘油月桂酸酯为10wt.％～20wt.％。In some embodiments, based on the total weight of the feed composition, the polyglycerol laurate is 0.1 wt.% to 60 wt.% (e.g., 0.1, 0.5, 1, 5, 10, 15, 20, 25 , 30, 35, 40, 45, 50, 55, 60wt.%). In some embodiments, based on the total weight of the feed composition, the polyglycerol laurate is 1 wt.% to 30 wt.%. In some embodiments, based on the total weight of the feed composition, the polyglyceryl laurate is 10 wt.% to 20 wt.%.

在一些实施方案中，以所述饲用组合物的总重量计，乳化剂为0wt.％～20wt.％(例如，0.1、0.5、1、5、10、15、20wt.％)。在一些实施方案中，以所述饲用组合物的总重量计，有机酸为0.01wt.％～10wt.％(例如，0.1、0.5、1、5、10wt.％)。在一些实施方案中，以所述饲用组合物的总重量计，调味剂为0wt.％～5wt.％(例如，0.1、0.5、1、5wt.％)。在一些实施方案中，以所述饲用组合物的总重量计，稳定剂为0wt.％～40wt.％(例如，0.1、0.5、1、5、10、15、20、25、30、35、40wt.％)。In some embodiments, based on the total weight of the feed composition, the emulsifier is 0 wt.% to 20 wt.% (eg, 0.1, 0.5, 1, 5, 10, 15, 20 wt.%). In some embodiments, the organic acid is 0.01 wt.% to 10 wt.% (for example, 0.1, 0.5, 1, 5, 10 wt.%) based on the total weight of the feed composition. In some embodiments, based on the total weight of the feed composition, the flavoring agent is 0 wt.% to 5 wt.% (for example, 0.1, 0.5, 1, 5 wt.%). In some embodiments, based on the total weight of the feed composition, the stabilizer is 0 wt.% to 40 wt.% (e.g., 0.1, 0.5, 1, 5, 10, 15, 20, 25, 30, 35 , 40wt.%).

作为非限制性的实例，酸性乳液的配方可以如下表所示：As a non-limiting example, the formula of the acid emulsion can be as shown in the following table:

聚甘油月桂酸单甘酯Polyglycerol monolaurate	0.1％-60％0.1%-60%
丁酸甘油酯Glyceryl Butyrate	0.1％-60％0.1%-60%
柠檬酸脂肪酸甘油酯Citric acid fatty acid glycerides	0％-5％0%-5%
硬脂酰乳酸钙(钠)Calcium stearoyl lactate (sodium)	0.05％-2％0.05%-2%
酪蛋白Casein	0％-8％0%-8%
单、双脂肪酸甘油酯Mono- and di-fatty acid glycerides	0.1％-5％0.1%-5%
有机酸Organic acid	0.02-5％0.02-5%
辛烯基琥珀酸淀粉钠Sodium starch octenyl succinate	0％-25％0%-25%
水water	按余量增减Increase or decrease by margin

作为更具体的非限制性的实例，酸性乳液的配方可以如下表所示：As a more specific non-limiting example, the formula of the acid emulsion can be as shown in the following table:

聚甘油月桂酸单甘酯Polyglycerol monolaurate	15％15%
丁酸甘油酯Glyceryl Butyrate	16％16%
柠檬酸脂肪酸甘油酯Citric acid fatty acid glycerides	1.3％1.3%
硬脂酰乳酸钙(钠)Calcium stearoyl lactate (sodium)	0.7％0.7%
酪蛋白Casein	1％1%
单、双脂肪酸甘油酯Mono- and di-fatty acid glycerides	0.8％0.8%
有机酸Organic acid	2％2%
辛烯基琥珀酸淀粉钠Sodium starch octenyl succinate	10％10%
水water	余量margin

在不存在冲突的情况下，第五方面所述的各项技术特征也适合于第六方面。In the absence of conflicts, the technical features described in the fifth aspect are also suitable for the sixth aspect.

第七方面，本申请提供了丁酸甘油酯和聚甘油月桂酸酯在制备饲用组合物中的用途，其中所述饲用组合物的pH值为2.5-6.0。In the seventh aspect, this application provides the use of glyceryl butyrate and polyglyceryl laurate in preparing a feed composition, wherein the pH of the feed composition is 2.5-6.0.

在不存在冲突的情况下，第五方面所述的各项技术特征也适合于第七方面。In the absence of conflicts, the technical features described in the fifth aspect are also suitable for the seventh aspect.

在第五至第七方面的一些实施方案中，饲用组合物用于预防或治疗动物中的微生物感染相关病症。In some embodiments of the fifth to seventh aspects, the feed composition is used to prevent or treat microbial infection-related disorders in animals.

适于给予本申请的饲用组合物的动物没有特别限制，合适的动物例如可以是家畜类、家禽类或水产类。Animals suitable for administering the feed composition of the present application are not particularly limited, and suitable animals may be, for example, livestock, poultry, or aquatic products.

本文中的“家畜”指养殖业中的哺乳动物，例如猪、牛、羊、狗、猫、兔等。"Livestock" in this article refers to mammals in the breeding industry, such as pigs, cattle, sheep, dogs, cats, rabbits, etc.

本文中的“家禽”指养殖业中的禽类动物，例如鸡、鸭、鹅以及鸟类养殖动物等。The "poultry" in this article refers to poultry animals in the breeding industry, such as chickens, ducks, geese, and bird breeding animals.

本文中的“水产类”指养殖业中的水产类动物，例如鱼、虾、蟹以及牛蛙等养殖动物。"Aquatic products" in this article refers to aquatic animals in the aquaculture industry, such as fish, shrimp, crabs, and bullfrogs.

在一些实施方案中，微生物感染为病毒感染或细菌感染。本文中的病毒或细菌可以禽畜患病或人畜共患的致病性病毒或细菌。In some embodiments, the microbial infection is a viral infection or a bacterial infection. The virus or bacteria herein can be pathogenic viruses or bacteria that are diseased in poultry and livestock or zoonotic.

囊膜(envelope)是指病毒外壳包被的由蛋白质、多糖和脂类构成的类脂双层膜，也称为包膜，主要来源于宿主细胞膜(磷脂层和膜蛋白)，但也包含有一些病毒自身的糖蛋白，主要功能是帮助病毒进入宿主细胞。Envelope refers to the lipid bilayer membrane composed of proteins, polysaccharides and lipids coated by the virus shell, also called envelope, which is mainly derived from the host cell membrane (phospholipid layer and membrane protein), but also contains The main function of some viruses' own glycoproteins is to help the virus enter the host cell.

在一些实施方案中，病毒可以为囊膜病毒，例如非洲猪瘟病毒(ASFV)、蓝耳病病毒、伪狂犬病毒、流感病毒、流行性腹泻病毒。In some embodiments, the virus may be an enveloped virus, such as African swine fever virus (ASFV), PRRS virus, pseudorabies virus, influenza virus, pandemic diarrhea virus.

在一些实施方案中，所述细菌可为沙门氏杆菌、大肠杆菌、金黄色葡萄球菌、空肠弯曲杆菌、梭菌。In some embodiments, the bacteria may be Salmonella, Escherichia coli, Staphylococcus aureus, Campylobacter jejuni, Clostridium.

实施例Example

下面结合实施例示例性描述本申请的发明，但实施例部分的内容不以任何方式限制本申请的各项发明。The inventions of the present application are exemplarily described below in conjunction with the embodiments, but the content of the embodiments does not limit the inventions of the present application in any way.

制备本申请的乳液组合物的原料配比如表1所示。Table 1 shows the ratio of raw materials for preparing the emulsion composition of the present application.

表1Table 1

制备工艺如下：The preparation process is as follows:

实施例1Example 1

制备本申请的乳液组合物的示例性原料配方如表2所示。Exemplary raw material formulations for preparing the emulsion composition of the present application are shown in Table 2.

表2Table 2

物料名称Material name	含量(克/千克)Content (g/kg)
三丁酸甘油酯Tributyrin	200200
月桂酸单甘油酯Lauric acid monoglyceride	100100
复合乳化剂Compound emulsifier	1515
硬脂酰乳酸钠Sodium Stearoyl Lactate	2020
变性淀粉Modified starch	150150
卡拉胶Carrageenan	22
丙酸Propionic acid	4040
三氯蔗糖Sucralose	55
水water	468468

根据表2中配方制备的乳液组合物pH＝3.65，用水稀释100倍后pH＝3.92，稀释500倍后pH＝3.98，稀释1000倍后pH＝4.23。The pH of the emulsion composition prepared according to the formula in Table 2 was 3.65, the pH was 3.92 after being diluted 100 times with water, the pH was 3.98 after being diluted 500 times, and the pH was 4.23 after being diluted 1000 times.

实施例2Example 2

制备本申请的乳液组合物的示例性原料配方如表3所示。Exemplary raw material formulations for preparing the emulsion composition of the present application are shown in Table 3.

表3table 3

物料名称Material name	含量(克/千克)Content (g/kg)
1-丁酸甘油酯1-butyric acid glyceride	200200
丁酸钠Sodium Butyrate	5050
月桂酸甘油酯Glyceryl laurate	150150
复合乳化剂Compound emulsifier	2020
双乙酰酒石酸单甘油酯Diacetyltartaric acid monoglyceride	1010
变性淀粉Modified starch	200200
卡拉胶Carrageenan	1.51.5
柠檬酸Citric acid	3030
阿斯巴甜aspartame	55
水water	333.5333.5

根据表3中配方制备的乳液组合物pH＝4.04，用水稀释100倍后pH＝4.57，稀释500倍后pH＝4.65，稀释1000倍后pH＝5.32。The pH of the emulsion composition prepared according to the formula in Table 3 was 4.04, the pH was 4.57 after being diluted 100 times with water, the pH was 4.65 after being diluted 500 times, and the pH was 5.32 after being diluted 1000 times.

实施例3Example 3

制备本申请的乳液组合物的示例性原料配方如表4所示。Exemplary raw material formulations for preparing the emulsion composition of the present application are shown in Table 4.

表4Table 4

物料名称Material name

含量(克/千克)Content (g/kg)

丁酸甘油酯Glyceryl Butyrate	00
单月桂酸甘油酯Glyceryl Monolaurate	300300
复合乳化剂Compound emulsifier	2020
酪蛋白Casein	3.53.5
变性淀粉Modified starch	100100
卡拉胶Carrageenan	1.01.0
乳酸Lactic acid	2020
蔗糖sucrose	5050
水water	505.5505.5

根据表4中配方制备的乳液组合物pH＝3.86，用水稀释100倍后pH＝4.65，稀释500倍后pH＝4.76，稀释1000倍后pH＝4.98。The pH of the emulsion composition prepared according to the formula in Table 4 was 3.86, the pH was 4.65 after being diluted 100 times with water, the pH was 4.76 after being diluted 500 times, and the pH was 4.98 after being diluted 1000 times.

根据实施例1-3中的配方均可制备均一稳定的酸乳，并且稀释后仍呈均一稳定的乳状液，可直接应用于畜禽饮水和饲料添加中。According to the formulas in Examples 1-3, uniform and stable yogurt can be prepared, and it is still a uniform and stable emulsion after dilution, which can be directly applied to livestock and poultry drinking water and feed addition.

实施例4：酸性乳液的制备Example 4: Preparation of acid emulsion

按照下表所示配方配制酸性乳液Prepare acid emulsion according to the formula shown in the table below

表5.table 5.

制备工艺如下：The preparation process is as follows:

将丁酸甘油酯、聚甘油月桂酸单甘酯加热到50-70℃左右，然后将乳化剂(柠檬酸脂肪酸甘油酯、硬脂酰乳酸钙(钠)、单、双脂肪酸甘油酯)按用量投入到丁酸甘油酯、聚甘油月桂酸单甘酯中溶解，保持温度50-80℃，作为油相待用；将辛烯基琥珀酸淀粉钠和酪蛋白按用量溶解如水中，并用水浴加热到80-95℃直到变成水溶液，然后降温冷却到70-80℃，待用；将有机酸、甜味剂(任选)按用量溶解后加入辛烯基琥珀酸淀粉钠和酪蛋白的混合溶液中，搅拌均匀，保持温度50-80℃，作为水相待用；将油相用输送泵缓慢输入水相中剪切混合初步乳化。初步乳化的混合液通过高压均质机，在15-45MPa均质压力下进行高压均质，高压均质后的乳状液进行灌装即可。Heat the butyric acid glyceride and polyglycerol lauric acid monoglyceride to about 50-70℃, and then use the emulsifier (citric acid fatty acid glyceride, stearoyl calcium lactate (sodium), mono- and di-fatty acid glycerides) according to the dosage Put into butyric acid glyceride, polyglyceryl lauric acid monoglyceride to dissolve, keep the temperature at 50-80℃, as the oil phase for later use; dissolve the sodium starch octenyl succinate and casein in water according to the amount, and heat in a water bath To 80-95 ℃ until it becomes an aqueous solution, then cool down to 70-80 ℃, set aside; dissolve the organic acid and sweetener (optional) according to the amount and add the sodium starch octenyl succinate and casein mixture In the solution, stir evenly, keep the temperature at 50-80°C, and serve as the water phase for later use; the oil phase is slowly fed into the water phase with a transfer pump for shear mixing and preliminary emulsification. The preliminarily emulsified mixture passes through a high-pressure homogenizer, and is homogenized at a homogenizing pressure of 15-45MPa, and the emulsion after high-pressure homogenization can be filled.

实施例5：酸性乳液的稀释pH测定Example 5: Dilution pH measurement of acid emulsion

本实施例测试了实施例4的酸性乳液在用水稀释不同倍数后的pH值。This example tested the pH value of the acid emulsion of Example 4 after being diluted with water by different multiples.

实施例6：酸性乳液的生物学评价Example 6: Biological evaluation of acid emulsion

本实施例对实施例4的酸性乳液进行了体外和体内的多种生物学评价。In this example, various biological evaluations in vitro and in vivo were performed on the acid emulsion of Example 4.

实施例6.1抗非洲猪瘟病毒(ASFV)细胞学测试Example 6.1 Anti-African Swine Fever Virus (ASFV) Cytological Test

在细胞水平上测试酸性乳液的抗非洲猪瘟病毒(ASFV)感染的功效，并同时引入月桂酸、月桂酸单甘脂和聚甘油月桂酸单甘酯进行比较。Test the efficacy of acid emulsion against African swine fever virus (ASFV) infection at the cellular level, and introduce lauric acid, lauric acid monoglyceride and polyglycerol lauric acid monoglyceride for comparison.

实验材料Experimental Materials

月桂酸、月桂酸单甘脂购自、聚甘油月桂酸单甘酯购自广州永易食品有限公司。Lauric acid and lauric acid monoglyceride were purchased from, and polyglycerol lauric acid monoglyceride was purchased from Guangzhou Yongyi Food Co., Ltd.

测试细胞为猪肺泡巨噬细胞，购自麒盟(上海)生物医学有限公司。The test cells were porcine alveolar macrophages, purchased from Qimeng (Shanghai) Biomedical Co., Ltd.

测试用非洲猪瘟病毒(ASFV)经遗传改造而携带荧光标记。The African swine fever virus (ASFV) for testing is genetically modified to carry a fluorescent label.

实验分组Experiment grouping

阴性对照组：仅添加细胞培养液。Negative control group: only add cell culture medium.

阳性对照组：添加混合有0.1MOI的病毒液的细胞培养液。Positive control group: add cell culture medium mixed with 0.1MOI virus liquid.

月桂酸组：将月桂酸先用少量二甲基亚砜溶解再用细胞培养液稀释成终浓度为1.4mg/ml、0.7mg/ml、0.28mg/ml的溶液，最后加入0.1MOI的病毒液充分混匀。同时设置不加病毒的对照。Lauric acid group: first dissolve lauric acid with a small amount of dimethyl sulfoxide and then dilute with cell culture solution to a final concentration of 1.4mg/ml, 0.7mg/ml, 0.28mg/ml, and finally add 0.1MOI of virus solution Mix well. At the same time, set a control without virus.

月桂酸单甘脂组：将月桂酸单甘脂先用少量二甲基亚砜溶解再用细胞培养液稀释成终浓度为1.92mg/ml、0.96mg/ml、0.384mg/ml(按照等月桂酸浓度折算分别为1.4mg/ml、0.7mg/ml、0.28mg/ml)的溶液，加入0.1MOI的病毒液充分混匀。同时设置不加病毒的对照。Lauric acid monoglyceride group: first dissolve lauric acid monoglyceride with a small amount of dimethyl sulfoxide and then dilute with cell culture solution to a final concentration of 1.92mg/ml, 0.96mg/ml, 0.384mg/ml (according to the The acid concentration was converted to 1.4mg/ml, 0.7mg/ml, 0.28mg/ml) solution, and 0.1MOI virus solution was added and mixed thoroughly. At the same time, set a control without virus.

聚甘油月桂酸单甘酯组：将聚甘油月桂酸单甘酯用细胞培养液稀释至终浓度为3mg/ml、1.5mg/ml、0.6mg/ml(按照等月桂酸浓度折算分别为1.4mg/ml、0.7mg/ml、0.28mg/ml)的溶液，加入0.1MOI的病毒液充分混匀。同时设置不加病毒的对照。Polyglycerol laurate monoglyceride group: Dilute polyglycerol laurate monoglyceride with cell culture solution to a final concentration of 3mg/ml, 1.5mg/ml, 0.6mg/ml (converted to 1.4mg according to the equivalent lauric acid concentration, respectively) /ml, 0.7mg/ml, 0.28mg/ml) solution, add 0.1MOI virus solution and mix well. At the same time, set a control without virus.

酸性乳液组：将酸性乳液用细胞培养液稀释至终浓度为40mg/ml、100mg/ml(按照等月桂酸浓度折算分别为2.8mg/ml、7mg/ml)的溶液，加入0.1MOI的病毒液充分混匀。同时设置不加病毒的对照。Acid emulsion group: Dilute the acid emulsion with cell culture solution to a final concentration of 40mg/ml, 100mg/ml (based on the equivalent lauric acid concentration to be 2.8mg/ml, 7mg/ml) solution, and add 0.1MOI of the virus solution Mix well. At the same time, set a control without virus.

实验流程experiment process

将细胞接种于96孔细胞培养板，生长至单层融合。在测试前，去除细胞培养液，按照“实验分组”中的描述分别加入配制好的测试样品，每孔各100μL，每组设置12个平行测试孔，置于37℃、5％CO ₂培养箱中孵育1h。 The cells were seeded on a 96-well cell culture plate and grown to a monolayer confluence. Before the test, remove the cell culture solution and add the prepared test samples separately according to the description in "Experimental Grouping", each 100μL per well, each group set up 12 parallel test holes, placed in 37℃, 5% CO ₂ incubator Incubate for 1h.

将孵育后的细胞培养孔内的液体弃掉，PBS洗涤后加入细胞维持培养基，置于37℃、5％CO ₂培养箱中培养72h，并在光学显微镜和荧光显微镜下分别观察细胞形态和病毒荧光。 Discard the liquid in the cell culture wells after incubation, add cell maintenance medium after washing with PBS, culture for 72 hours in a 37°C, 5% CO ₂ incubator, and observe the cell morphology and cell morphology under an optical microscope and a fluorescence microscope, respectively Virus fluorescence.

实验结果Experimental results

示例性照片显示于图1-13中，并总结于下表中。Exemplary photos are shown in Figures 1-13 and are summarized in the table below.

表6.各组的细胞形态学和病毒荧光结果总结Table 6. Summary of cell morphology and virus fluorescence results of each group

根据图1和图2的结果可知，本实施例的细胞评价模型是成功的。According to the results of Figure 1 and Figure 2, it can be seen that the cell evaluation model of this example is successful.

由于病毒复制会受到细胞自身增殖、生长情况的影响，因此对于病毒抑制功效的评价需要考虑这方面的结果。Since virus replication is affected by the proliferation and growth of cells, the evaluation of virus inhibitory efficacy needs to consider the results of this aspect.

月桂酸的3个组不能实现同时保持较好的细胞形态学且实现有效病毒抑制。The three groups of lauric acid could not achieve while maintaining good cell morphology and achieving effective virus suppression.

月桂酸单甘脂1.92mg/ml和0.96mg/ml浓度组存在细胞毒性，0.384mg/ml浓度组的细胞毒性不明显，并且存在一定的病毒抑制效果。There was cytotoxicity in the 1.92mg/ml and 0.96mg/ml concentration groups of lauric acid monoglyceride, and the 0.384mg/ml concentration group had no obvious cytotoxicity, and there was a certain virus inhibitory effect.

聚甘油月桂酸单甘酯3mg/ml和1.5mg/ml浓度组存在细胞毒性，0.6mg/ml浓度组的细胞毒性不明显，并且存在一定的病毒抑制效果。The 3mg/ml and 1.5mg/ml concentration groups of polyglyceryl laurate monoglyceride showed cytotoxicity, and the 0.6mg/ml concentration group had no obvious cytotoxicity, and there was a certain virus inhibitory effect.

酸性乳液40mg/ml浓度组无明显细胞毒性且实现了强病毒抑制效果，100mg/ml浓度组仅具有轻微细胞毒性且实现了强病毒抑制效果。The acid emulsion 40mg/ml concentration group has no obvious cytotoxicity and achieves a strong virus suppression effect, while the 100mg/ml concentration group has only slight cytotoxicity and achieves a strong virus suppression effect.

综合以上结果，酸性乳液在抗ASFV方面的作用和效果大大优于月桂酸、月桂酸单甘脂和聚甘油月桂酸单甘酯。值得注意的是，按照等月桂酸浓度折算，酸性乳液40mg/ml浓度组是月桂酸、月桂酸单甘脂和聚甘油月桂酸单甘酯的最高浓度组的2倍，这提示酸性乳液的配制能够显著降低月桂酸的细胞毒性。本申请开发的乳液中联合使用了丁酸甘油酯和聚甘油月桂酸单甘酯并将其制备成酸性形式，这样的产品实现了优异的抗病毒结果。Based on the above results, the effect and effect of acid emulsion in anti-ASFV is much better than lauric acid, lauric acid monoglyceride and polyglycerol lauric acid monoglyceride. It is worth noting that according to the equivalent lauric acid concentration, the 40mg/ml acid emulsion concentration group is twice the highest concentration group of lauric acid, lauric acid monoglyceride and polyglycerol lauric acid monoglyceride, which suggests the preparation of acid emulsion Can significantly reduce the cytotoxicity of lauric acid. The emulsion developed in the present application combines butyric acid glyceride and polyglyceryl lauric acid monoglyceride and prepares it into an acid form. Such a product achieves excellent antiviral results.

实施例6.2治疗断奶仔猪腹泻Example 6.2 Treatment of diarrhea in weaned piglets

湖南省岳阳市柏祥镇一养猪场，存栏母猪500多头，仔猪断奶第2天开始腹泻，多种化学药物治疗无效，确定为病毒性腹泻或营养性腹泻。There are more than 500 sows in a pig farm in Baixiang Town, Yueyang City, Hunan Province. The piglets started to have diarrhea on the second day of weaning. Many chemical drugs were ineffective and it was determined to be viral diarrhea or nutritional diarrhea.

将酸性乳液均匀施加于饲料，供仔猪自由进食(估算酸性乳液的摄入量为30ml/天/头)，第2天仔猪腹泻症状减轻，第3天基本康复，提示酸性乳液对于治疗和缓解腹泻(疑似病毒性)具有明显的作用。The acid emulsion is evenly applied to the feed for the piglets to eat freely (estimated intake of acid emulsion is 30ml/day/head), the piglets' diarrhea symptoms are alleviated on the 2nd day, and the piglets basically recovered on the 3rd day, suggesting that the acid emulsion is useful for the treatment and alleviation of diarrhea (Suspected of viral) has obvious effects.

实施例6.3治疗仔猪多***衰竭症(PMWS)Example 6.3 Treatment of multiple system failure in piglets (PMWS)

山西省原平市大营育肥场有3000头保育猪患仔猪多***衰竭症(PMWS)，同时继发感染胸膜肺炎和支原体。In Daying Fattening Farm, Yuanping City, Shanxi Province, 3,000 nursery pigs were suffering from multiple system failure (PMWS) in piglets and were also infected with pleuropneumonia and mycoplasma.

从中挑出178头病程较长(超过一个月)、消瘦严重(25-30kg)、药物治疗无效的病猪。Among them, 178 sick pigs with a long course of disease (more than one month), severe weight loss (25-30 kg) and ineffective drug treatment were selected.

将酸性乳液均匀施加于饲料，供仔猪自由进食(估算酸性乳液的摄入量为30ml/天/头)，施用2天后仔猪的精神状况和体况明显好转，采食量显著提升，死亡率明显降低，具体结果如下。Evenly apply the acid emulsion to the feed for the piglets to eat freely (estimated intake of the acid emulsion is 30ml/day/head). After 2 days of application, the mental and physical condition of the piglets are significantly improved, the feed intake is significantly increased, and the mortality rate is obvious The specific results are as follows.

表7.仔猪死亡情况记录表Table 7. Piglet death record table

实验期Experimental period	第1天Day 1	第2天Day 2	第3天3rd day	第4天Day 4	第5天Day 5	第6天Day 6	第7天Day 7
死亡数Number of deaths	1212	55	44	1010	99	77	66
死亡率mortality rate	7％7%	3％3%	3％3%	6％6%	6％6%	5％5%	4.5％4.5%

根据育肥场工作人员的介绍，在本实验前，仔猪的每日死亡率大约为20％。在摄入酸性乳液后，每日死亡率明显降低，最高为7％。According to the staff of the fattening farm, before this experiment, the daily mortality rate of piglets was about 20%. After ingesting the acid emulsion, the daily mortality rate was significantly reduced, up to 7%.

表8仔猪采食量情况记录表Table 8 Record table of piglet feed intake

从表8的结果可以看出，随着实验的进行，病猪的平均采食量呈上升的趋势，从摄入酸性乳液后的第一天平均采食量0.21kg到第7天平均采食量达到0.4kg，采食量提高了90.5％。It can be seen from the results in Table 8 that with the progress of the experiment, the average feed intake of sick pigs showed an upward trend, from the average feed intake of 0.21 kg on the first day after ingesting the acid emulsion to the average feed intake on the seventh day The amount reached 0.4kg, and the feed intake increased by 90.5%.

实施例6.4治疗禽白痢Example 6.4 Treatment of Avian Pullorum

湖南省岳阳县一养鸡场购进鸡苗10000羽，在第5日，发现50余只雏鸡死亡，经解剖和临床确认为沙门氏杆菌感染。A chicken farm in Yueyang County, Hunan Province purchased 10,000 chicks. On the 5th day, more than 50 chicks were found dead, which were confirmed to be infected with Salmonella after anatomy and clinical trials.

在第6日和第7日，以约1:1000的比例将酸性乳液混入雏鸡饮水中。第6日和第7日雏鸡死亡数分别为130和200余只。On the 6th and 7th day, the acid emulsion was mixed into the chicken drinking water at a ratio of about 1:1000. The number of chicks that died on the 6th and 7th day was 130 and more than 200, respectively.

自第7日夜间，将稀释比例改为约1:600，第8日雏鸡降至30余只。连续施用3天，死亡率进一步明显降低。From the night of the 7th day, the dilution ratio was changed to about 1:600, and on the 8th day the chicks were reduced to more than 30. After 3 days of continuous administration, the mortality rate was further reduced significantly.

实施例6.5治疗禽水便Example 6.5 Treatment of poultry water stool

广西省玉林市一养鸡场禽存栏量为16～17万羽，主要以三黄鸡为主。工作人员发现鸡的粪便比较稀，不成型。The number of poultry on a chicken farm in Yulin City, Guangxi Province is 160,000 to 170,000 birds, mainly three yellow chickens. The staff found that the chicken feces were relatively thin and not forming.

以约1:700的比例将酸性乳液混入鸡的饮水中，连续施用3天后水便明显减少，粪便更加成型(结果如图14所示)。The acid emulsion was mixed into the chicken's drinking water at a ratio of about 1:700. After 3 days of continuous application, the water and stool were significantly reduced and the stool was more formed (the result is shown in Figure 14).

实施例6.6：抗流感病毒细胞学测试Example 6.6: Anti-influenza virus cytology test

在细胞水平上测试酸性乳液的抗H1N1流感病毒感染的功效，并同时引入月桂酸、月桂酸单甘酯和聚甘油月桂酸单甘酯进行比较。Test the effect of acid emulsion against H1N1 influenza virus infection at the cellular level, and introduce lauric acid, lauric acid monoglyceride and polyglycerol lauric acid monoglyceride for comparison.

1实验材料1 Experimental materials

月桂酸购自广州永易食品有限公司Lauric acid was purchased from Guangzhou Yongyi Food Co., Ltd.

月桂酸单甘酯购自广州永易食品有限公司Monoglyceride laurate was purchased from Guangzhou Yongyi Food Co., Ltd.

聚甘油月桂酸单甘酯购自广州永易食品有限公司Polyglycerol monolaurate was purchased from Guangzhou Yongyi Food Co., Ltd.

A549细胞购自麒盟(上海)生物医学有限公司A549 cells were purchased from Qimeng (Shanghai) Biomedical Co., Ltd.

2实验步骤2 experimental steps

2.1实验样品处理2.1 Experimental sample processing

月桂酸组(A)：将月桂酸先用少量二甲基亚砜溶解再用细胞培养液稀释成终浓度为0.35mg/ml。Lauric acid group (A): first dissolve lauric acid with a small amount of dimethyl sulfoxide and then dilute with cell culture solution to a final concentration of 0.35 mg/ml.

月桂酸单甘酯组(B)：将月桂酸单甘酯先用少量二甲基亚砜溶解再用细胞培养液稀释成终浓度为0.48mg/ml(按照等月桂酸浓度折算分别为0.35mg/ml)。Monolauric acid monoglyceride group (B): first dissolve lauric acid monoglyceride with a small amount of dimethyl sulfoxide and then dilute with cell culture medium to a final concentration of 0.48mg/ml (converted to 0.35mg according to the equivalent lauric acid concentration) /ml).

聚甘油月桂酸单甘酯组(C)：将聚甘油月桂酸单甘酯用细胞培养液稀释至终浓度为0.75mg/ml(按照等月桂酸浓度折算分别为0.35mg/ml)。Polyglycerol laurate monoglyceride group (C): Dilute polyglycerol laurate monoglyceride with cell culture fluid to a final concentration of 0.75 mg/ml (converted to 0.35 mg/ml according to the equivalent lauric acid concentration).

酸性乳液组(D)：将酸性乳液用细胞培养液稀释至终浓度为5mg/ml(按照等月桂酸浓度折算分别为0.35mg/ml)。Acid emulsion group (D): Dilute the acid emulsion with cell culture solution to a final concentration of 5 mg/ml (converted to 0.35 mg/ml according to the equivalent lauric acid concentration).

阳性对照组(V)：添加混合有2 ⁴HAU的流感病毒的细胞培养液。 Positive control group (V): Add cell culture medium mixed with ²⁴ HAU influenza virus.

2.2病毒接种2.2 Virus inoculation

24孔板培养A549细胞，细胞长好后感染流感病毒，并用2.1的样品处理(分两种处理方式，一种是样品和病毒同时接种细胞1小时，一种是细胞感染病毒1小时后再加样品处理1小时)，24小时后取上清做血凝实验，收取细胞提取RNA进行qRT-PCR。Culture A549 cells in a 24-well plate. After the cells grow well, they are infected with influenza virus and treated with the sample of 2.1 (two treatment methods, one is to inoculate the cells with the sample and the virus for 1 hour, and the other is to add the virus after 1 hour of infection. Sample processing for 1 hour), after 24 hours, the supernatant was taken for hemagglutination experiment, and the cells were collected to extract RNA for qRT-PCR.

3实验结果3 experimental results

V表示病毒组，V-表示先感染病毒1小时再加样品处理1小时，V+表示病毒和样品同时接种细胞。V indicates the virus group, V- indicates that the virus is infected for 1 hour and then the sample is treated for 1 hour, and V+ indicates that the virus and the sample are simultaneously inoculated with cells.

3.1血凝实验结果3.1 Hemagglutination test results

表9Table 9

处理deal with

AA

BB

CC

DD

V-V-	4、54, 5	2、32, 3	4、44, 4	3、43, 4
V+V+	5、55, 5	2、22, 2	4、54, 5	2、32, 3
平均值average value	4.754.75	2.252.25	4.254.25	33

备注：阳性对照病毒组(V)血凝价为8、9。Remarks: The hemagglutination value of the positive control virus group (V) is 8, 9.

由表9的结果可见，两种处理方式血凝价平均结果从低到高为：B〈D〈C〈A，血凝价越高说明病毒含量越高，由此可知B组对流感病毒的抑制效果最强，其次是D组。It can be seen from the results in Table 9 that the average blood clotting value of the two treatments is from low to high: B<D<C<A. The higher the blood clotting value, the higher the virus content. It can be seen that the group B has an effect on influenza virus. The inhibitory effect is the strongest, followed by group D.

3.2 qRT-PCR检测3.2 qRT-PCR detection

对于核蛋白(NP)的qRT-PCR检测结果与上文血凝实验结果趋势相同，病毒含量从低到高为：B〈D〈C〈A，由此可知B组对流感病毒的抑制效果最强，其次是D组。The qRT-PCR detection results for nucleoprotein (NP) have the same trend as the results of the hemagglutination experiment above. The virus content from low to high is: B<D<C<A, which shows that group B has the most inhibitory effect on influenza virus Strong, followed by Group D.

血凝实验和qRT-PCR的检测结果均表明，本申请的酸性乳液对H1N1流感病毒具有较强的抑制效果。Both the hemagglutination test and the qRT-PCR test results show that the acid emulsion of the present application has a strong inhibitory effect on the H1N1 influenza virus.

实施例6.7：种禽生产性能测试Example 6.7: Breeder production performance test

在广西一种鸡场，选择一栋202日龄的种蛋鸡舍，分三条饲养线，将三条线随机分成两个处理组，其中一条线为实验组，种蛋鸡数量1392羽，饲喂基础日粮，另外饮水添加酸乳1kg/吨，自由饮用；另外两条线为对照组，种蛋鸡数量2383羽，饲喂基础日粮，自由饮水。试验期30天。结果如下：In a kind of chicken farm in Guangxi, choose a 202-day-old breeder house, divide it into three feeding lines, and randomly divide the three lines into two treatment groups, one of which is the experimental group, the number of breeders is 1,392, and the feeding basis In the diet, 1kg/ton of yogurt was added to the drinking water, free drinking; the other two lines were the control group, the number of laying hens was 2,383, and the basic diet was fed with free drinking. The test period is 30 days. The results are as follows:

表10 种蛋鸡生产性能情况表Table 10 Production performance of breeding layer

由表10结果可见，试验组破蛋率、次蛋率和死淘率跟对照组相比明显改善了，分别降低了23％、13.33％、46.34％。说明添加酸乳可以提高种蛋鸡的生产性能。From the results in Table 10, it can be seen that the egg broken rate, defective egg rate and dead scouring rate of the experimental group were significantly improved compared with the control group, which were reduced by 23%, 13.33%, and 46.34% respectively. It shows that adding yogurt can improve the production performance of the breeding layer.

对蛋的品质进行肉眼观察可见，试验组的蛋黄颜色明显比对照组的颜色深，蛋清也明显比对照组粘稠，说明添加酸乳可以改善蛋品质，使蛋黄颜色更深，蛋清粘稠度(哈夫单位)更高。Visual observation of the egg quality shows that the egg yolk color of the test group is obviously darker than that of the control group, and the egg white is also significantly thicker than the control group, indicating that adding yogurt can improve the egg quality, making the egg yolk darker, and the egg white consistency ( Huff unit) is higher.

实施例6.8：肉鸡生产性能测试Example 6.8: Broiler production performance test

在山西一大型肉鸡养殖场，选择两栋22日龄的白羽肉鸡(哈巴德)进行试验，分为试验组和对照组，试验组34173羽，饲喂基础日粮，并饮水添加酸乳1L/吨，4次/天，自由饮水。对照组34174羽，饲喂基础日粮，自由饮水。试验至肉鸡出栏。 In a large broiler farms Shanxi, select two 22-day-old broiler white feather (Hubbard) test, the test group and the control group, the experimental group 34,173 birds fed the basal diet and drinking yoghurt add 1L /Ton, 4 times/day, free drinking water. The control group was 34174 pigeons fed with basal diet and free drinking water. Test until the broiler is released.

表11.肉鸡死淘率对比情况表Table 11. Comparison table of broiler death rate

由表11结果可见，与对照组相比，饮水中添加酸乳显著降低了试验组各阶段的死淘率。It can be seen from the results in Table 11 that, compared with the control group, the addition of yogurt in the drinking water significantly reduced the rate of death in each stage of the test group.

表12.肉鸡生产性能对比情况表Table 12. Comparison of broiler production performance

由表12结果可见，试验组的初始体重比对照组低22g，使用酸乳两周后的平均日增重超过对照组，至46日龄时，试验组均重比对照组高60.77g，酸乳促进肉鸡生长的效果明显。It can be seen from the results in Table 12 that the initial weight of the experimental group was 22g lower than that of the control group. The average daily gain after using yogurt for two weeks exceeded that of the control group. At 46 days of age, the average weight of the experimental group was 60.77g higher than the control group. Milk promotes the growth of broilers.

上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。In the above, the present invention has been described in detail with general descriptions and specific embodiments, but on the basis of the present invention, some modifications or improvements can be made, which is obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention belong to the scope of the present invention.

Claims

饲用乳液组合物，其包含Feed emulsion composition, which comprises

丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或Butyrate, butyrin, butyrin derivatives or mixtures thereof, and/or

月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物，Glyceryl laurate, glyceryl laurate derivatives or mixtures thereof,

其中所述饲用乳液组合物的pH值为2-6，优选为2.5-5.5。The pH of the feed emulsion composition is 2-6, preferably 2.5-5.5.
如权利要求1所述的饲用乳液组合物，其中The feed emulsion composition of claim 1, wherein

所述丁酸盐选自钠盐、钾盐、钙盐、镁盐以及它们的任意组合；和/或The butyrate is selected from sodium salt, potassium salt, calcium salt, magnesium salt and any combination thereof; and/or

所述丁酸甘油酯选自三丁酸甘油酯、单丁酸甘油酯、双丁酸甘油酯以及它们的任意组合；和/或The glyceryl butyrate is selected from the group consisting of tributyrin, glyceryl monobutyrate, glyceryl dibutyrate and any combination thereof; and/or

所述丁酸甘油酯衍生物选自丁酸月桂酸甘油酯，丁酸辛酸甘油酯，丁酸癸酸甘油酯，丁酸辛酸癸酸甘油酯，丁酸、辛酸、癸酸和月桂酸与甘油的组配甘油酯，以及它们的任意组合。The butyric acid glyceride derivative is selected from the group consisting of butyric acid lauric acid glyceride, butyric acid caprylic glyceride, butyric acid capric glyceride, butyric acid caprylic acid capric glyceride, butyric acid, caprylic acid, capric acid and lauric acid and glycerin The combination of glycerides, and any combination of them.
如权利要求1或2所述的饲用乳液组合物，其中The feed emulsion composition of claim 1 or 2, wherein

所述月桂酸甘油酯选自聚甘油月桂酸酯、单月桂酸甘油酯、双月桂酸甘油酯、三月桂酸甘油酯以及它们的任意组合；和/或The glyceryl laurate is selected from the group consisting of polyglyceryl laurate, glyceryl monolaurate, glyceryl dilaurate, glyceryl trilaurate, and any combination thereof; and/or

所述月桂酸甘油酯衍生物选自月桂酸和其他短中长链脂肪酸与甘油的酯化物。The lauric acid glyceride derivative is selected from esters of lauric acid and other short, medium and long chain fatty acids with glycerol.
如权利要求1-3中任一项所述的饲用乳液组合物，其中所述饲用乳液组合物在用水稀释100-1000倍后的pH值为3-6，优选为3.5-5.5。The feed emulsion composition according to any one of claims 1 to 3, wherein the pH of the feed emulsion composition after being diluted 100-1000 times with water is 3-6, preferably 3.5-5.5.
制备饲用乳液组合物的方法，包括以下步骤：The method for preparing a feed emulsion composition includes the following steps:

将丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物，与乳化剂、稳定剂、有机酸和甜味剂进行乳化均质处理；Combine butyrate, butyric acid glyceride, butyric acid glyceride derivatives or mixtures thereof, and/or lauric acid glyceride, lauric acid glycerides derivatives or mixtures thereof, with emulsifiers, stabilizers, organic acids and sweeteners Emulsify and homogenize the agent;

其中，通过所述方法制备的饲用乳液组合物的pH值为2-6，优选为2.5-5.5。Wherein, the pH of the feed emulsion composition prepared by the method is 2-6, preferably 2.5-5.5.
如权利要求5所述的方法，其中The method of claim 5, wherein

所述丁酸盐选自钠盐、钾盐、钙盐、镁盐以及它们的任意组合；和/或The butyrate is selected from sodium salt, potassium salt, calcium salt, magnesium salt and any combination thereof; and/or

所述丁酸甘油酯选自三丁酸甘油酯、单丁酸甘油酯、双丁酸甘油酯以及它们的任意组合；和/或The glyceryl butyrate is selected from the group consisting of tributyrin, glyceryl monobutyrate, glyceryl dibutyrate and any combination thereof; and/or

所述丁酸甘油酯衍生物选自丁酸月桂酸甘油酯，丁酸辛酸甘油酯，丁酸癸酸甘油酯，丁酸辛酸癸酸甘油酯，丁酸、辛酸、癸酸和月桂酸与甘油的组配甘油酯，以及它们的任意组合；和/或The butyric acid glyceride derivative is selected from the group consisting of butyric acid lauric acid glyceride, butyric acid caprylic glyceride, butyric acid capric glyceride, butyric acid caprylic acid capric glyceride, butyric acid, caprylic acid, capric acid and lauric acid and glycerin Glycerides of, and any combination of them; and/or

所述月桂酸甘油酯选自聚甘油月桂酸酯、单月桂酸甘油酯、双月桂酸甘油酯、三月桂酸甘油酯以及它们的任意组合；和/或The glyceryl laurate is selected from the group consisting of polyglyceryl laurate, glyceryl monolaurate, glyceryl dilaurate, glyceryl trilaurate, and any combination thereof; and/or

所述月桂酸甘油酯衍生物选自月桂酸和其他短中长链脂肪酸与甘油的酯化物；和/或The lauric acid glyceride derivative is selected from esters of lauric acid and other short, medium and long chain fatty acids with glycerol; and/or

所述乳化剂选自硬酯酰乳酸钠、复合乳化剂、双乙酰酒石酸单甘油酯、以及它们的任意组合，其中所述复合乳化剂包含甘油脂肪酸脂和蔗糖脂肪酸脂及硬脂酰乳酸钠-钙；和/或The emulsifier is selected from sodium stearoyl lactylate, a complex emulsifier, diacetyl tartaric acid monoglyceride, and any combination thereof, wherein the complex emulsifier includes glycerol fatty acid ester, sucrose fatty acid ester and sodium stearoyl lactylate-calcium; and / or

所述稳定剂选自酪蛋白、变性淀粉、卡拉胶以及它们的任意组合；和/或The stabilizer is selected from casein, modified starch, carrageenan and any combination thereof; and/or

所述有机酸选自丙酸、柠檬酸、乳酸以及它们的任意组合；和/或The organic acid is selected from propionic acid, citric acid, lactic acid and any combination thereof; and/or

所述甜味剂选自三氯蔗糖、阿斯巴甜、蔗糖以及它们的任意组合。The sweetener is selected from sucralose, aspartame, sucrose and any combination thereof.
如权利要求5或6所述的方法，其中以所述饲用乳液组合物的总重量计，其中The method of claim 5 or 6, wherein based on the total weight of the feed emulsion composition, wherein

所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物为0wt.％～80wt.％；和/或The butyrate, butyric acid glyceride, butyric acid glyceride derivatives or mixtures thereof are 0wt.% to 80wt.%; and/or

所述月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物为0wt.％～80wt.％；和/或The said lauric acid glyceride, lauric acid glyceride derivative or a mixture thereof is 0wt.%～80wt.%; and/or

所述乳化剂为0wt.％～20wt.％；和/或The emulsifier is 0wt.%-20wt.%; and/or

所述有机酸为0.01wt.％～10wt.％；和/或The organic acid is 0.01wt.% to 10wt.%; and/or

所述甜味剂为0wt.％～5wt.％；和/或The sweetener is 0wt.%～5wt.%; and/or

所述稳定剂为0wt.％～58wt.％。The stabilizer is 0 wt.% to 58 wt.%.
如权利要求5至7中任一项所述的方法，包括以下步骤：The method according to any one of claims 5 to 7, comprising the following steps:

将乳化剂溶解于丁酸甘油酯、丁酸甘油酯衍生物或其混合物，和/或月桂酸甘油酯、月桂酸甘油酯衍生物或其混合物中，得到油相；Dissolving the emulsifier in butyrin, butyrin derivatives or mixtures thereof, and/or lauric acid glycerides, lauric acid glycerides derivatives or mixtures thereof to obtain an oil phase;

将稳定剂、有机酸和甜味剂溶解并混合，得到水相；Dissolve and mix the stabilizer, organic acid and sweetener to obtain an aqueous phase;

对水相和油相进行混合、乳化、均质化处理。The water phase and oil phase are mixed, emulsified, and homogenized.
如权利要求5-8中任一项所述的方法，其中The method of any one of claims 5-8, wherein

所述乳化剂与所述稳定剂的质量比为1：(4-11)，优选地，卡拉胶、变性淀粉、复合乳化剂与双乙酰酒石酸单甘油酯的质量比为1：(70-140)：(7-25)：(0-7)；和/或The mass ratio of the emulsifier and the stabilizer is 1: (4-11), preferably, the mass ratio of carrageenan, modified starch, composite emulsifier and diacetyl tartrate monoglyceride is 1: (70-140 ): (7-25): (0-7); and/or

所述甜味剂、所述有机酸与所述丁酸盐、丁酸甘油酯、丁酸甘油酯衍生物或其混合物的质量比为1：(0.1-10)：(0-60)。The mass ratio of the sweetener, the organic acid and the butyrate, butyric acid glyceride, butyric acid glyceride derivative or mixture thereof is 1: (0.1-10): (0-60).
权利要求1至4中任一项所述的饲用乳液组合物或根据权利要求5-9中任一项所述的方法制备的饲用乳液组合物在动物饲养中的用途；优选地，所述动物选自家畜类、家禽类或水产类(例如鱼类)。Use of the feed emulsion composition of any one of claims 1 to 4 or the feed emulsion composition prepared according to the method of any one of claims 5-9 in animal feeding; preferably, The animal is selected from livestock, poultry or aquatic products (such as fish).
权利要求1至4中任一项所述的饲用乳液组合物或根据权利要求5-9中任一项所述的方法制备的饲用乳液组合物在制备用于预防或治疗诸如家畜类、家禽类或水产类的动物中的微生物感染相关病症的药物中的用途。The feed emulsion composition of any one of claims 1 to 4 or the feed emulsion composition prepared according to the method of any one of claims 5-9 is prepared for the prevention or treatment of livestock, Use in medicine for diseases related to microbial infections in poultry or aquatic animals.
如权利要求11所述的用途，其中所述微生物感染为病毒感染或细菌感染，任选地，The use according to claim 11, wherein the microbial infection is a viral infection or a bacterial infection, optionally,

所述病毒为囊膜病毒，例如非洲猪瘟病毒(ASFV)、蓝耳病病毒、伪狂犬病毒、流感病毒、流行性腹泻病毒；或The virus is an enveloped virus, such as African swine fever virus (ASFV), PRRS virus, pseudorabies virus, influenza virus, epidemic diarrhea virus; or

所述细菌选自沙门氏杆菌、大肠杆菌、金黄色葡萄球菌、空肠弯曲杆菌、梭菌。The bacteria are selected from Salmonella, Escherichia coli, Staphylococcus aureus, Campylobacter jejuni and Clostridium.
饲用组合物，所述饲用组合物包含丁酸甘油酯和聚甘油月桂酸酯，并且pH值为2.5-6.0。The feed composition contains butyric acid glyceride and polyglyceryl laurate, and has a pH value of 2.5-6.0.
如权利要求13所述的饲用组合物，其中The feed composition of claim 13, wherein

所述丁酸甘油酯选自一丁酸甘油酯、二丁酸甘油酯、三丁酸甘油酯或以上的任意组合；和/或The glyceryl butyrate is selected from glyceryl monobutyrate, glyceryl dibutyrate, tributyrate or any combination thereof; and/or

所述聚甘油月桂酸酯为聚甘油月桂酸甘油酯，优选为聚甘油月桂酸单甘酯。The polyglycerol laurate is polyglycerol laurate, preferably polyglycerol laurate monoglyceride.
如权利要求13或14所述的饲用组合物，其中所述饲用组合物为酸性乳液的形式，pH值为2.5-3.0，优选地，所述酸性乳液包含乳化剂、稳定剂、有机酸和调味剂中的一种或多种。The feed composition according to claim 13 or 14, wherein the feed composition is in the form of an acid emulsion with a pH value of 2.5-3.0. Preferably, the acid emulsion comprises an emulsifier, a stabilizer, and an organic acid. And one or more of flavoring agents.
制备饲用组合物的方法，所述饲用组合物为包含丁酸甘油酯和聚甘油月桂酸酯的酸性乳液的形式，并且pH值为2.5-3.0，所述方法包括：A method for preparing a feed composition, the feed composition is in the form of an acid emulsion containing butyric acid glyceride and polyglyceryl laurate and has a pH of 2.5-3.0, the method comprising:

将丁酸甘油酯、聚甘油月桂酸酯与乳化剂、稳定剂、有机酸和任选的调味剂进行乳化均质处理。Glyceryl butyrate, polyglyceryl laurate, emulsifier, stabilizer, organic acid and optional flavoring agent are emulsified and homogenized.
如权利要求15所述的饲用组合物或权利要求16所述的方法，其中，The feed composition of claim 15 or the method of claim 16, wherein:

所述乳化剂选自柠檬酸脂肪酸甘油酯、硬酯酰乳酸钠、硬酯酰乳酸钙、单/双脂肪酸甘油酯、以及它们的任意组合；和/或The emulsifier is selected from citric acid fatty acid glycerides, sodium stearoyl lactylate, calcium stearoyl lactate, mono/di fatty acid glycerides, and any combination thereof; and/or

所述稳定剂选自酪蛋白、变性淀粉(例如辛烯基琥珀酸淀粉钠)、卡拉胶以及它们的任意组合；和/或The stabilizer is selected from casein, modified starch (such as sodium starch octenyl succinate), carrageenan and any combination thereof; and/or

所述有机酸选自丙酸、柠檬酸、乳酸以及它们的任意组合；和/或The organic acid is selected from propionic acid, citric acid, lactic acid and any combination thereof; and/or

所述调味剂选自三氯蔗糖、阿斯巴甜、蔗糖以及它们的任意组合。The flavoring agent is selected from sucralose, aspartame, sucrose and any combination thereof.
如权利要求17所述的饲用组合物或方法，其中以所述饲用组合物的总重量计，其中The feed composition or method of claim 17, wherein based on the total weight of the feed composition, wherein

所述丁酸甘油酯为0.1wt.％～60wt.％；和/或The butyric acid glyceride is 0.1wt.% to 60wt.%; and/or

所述聚甘油月桂酸酯为0.1wt.％～60wt.％；和/或The polyglycerol laurate is 0.1 wt.% to 60 wt.%; and/or

所述乳化剂为0wt.％～20wt.％；和/或The emulsifier is 0wt.%-20wt.%; and/or

所述有机酸为0.01wt.％～10wt.％；和/或The organic acid is 0.01wt.% to 10wt.%; and/or

所述调味剂为0wt.％～5wt.％；和/或The flavoring agent is 0wt.%～5wt.%; and/or

所述稳定剂为0wt.％～40wt.％。The stabilizer is 0wt.%-40wt.%.
丁酸甘油酯和聚甘油月桂酸酯在制备饲用组合物中的用途，其中所述饲用组合物的pH值为2.5-6.0，优选地，The use of butyric acid glyceride and polyglyceryl laurate in the preparation of a feed composition, wherein the pH of the feed composition is 2.5-6.0, preferably,

所述丁酸甘油酯选自一丁酸甘油酯、二丁酸甘油酯、三丁酸甘油酯或以上的任意组合；和/或The glyceryl butyrate is selected from glyceryl monobutyrate, glyceryl dibutyrate, tributyrate or any combination thereof; and/or

所述聚甘油月桂酸酯为聚甘油月桂酸甘油酯，优选为聚甘油月桂酸单甘酯。The polyglycerol laurate is polyglycerol laurate, preferably polyglycerol laurate monoglyceride.
如权利要求19所述的用途，其中所述饲用组合物为酸性乳液的形式，pH值为2.5-3.0，优选地，所述酸性乳液包含乳化剂、稳定剂、有机酸和调味剂中的一种或多种。The use according to claim 19, wherein the feed composition is in the form of an acid emulsion with a pH of 2.5-3.0. Preferably, the acid emulsion contains emulsifiers, stabilizers, organic acids and flavoring agents. One or more.
如权利要求19或20所述的用途，所述饲用组合物用于预防或治疗诸如家畜类、家禽类或水产类的动物中的微生物感染相关病症。The use according to claim 19 or 20, the feed composition is used to prevent or treat diseases related to microbial infection in animals such as livestock, poultry or aquatic products.
如权利要求21所述的用途，其中所述微生物感染为病毒感染或细菌感染，任选地，The use according to claim 21, wherein the microbial infection is a viral infection or a bacterial infection, optionally,

所述病毒为囊膜病毒，例如非洲猪瘟病毒(ASFV)、蓝耳病病毒、伪狂犬病毒、流感病毒、流行性腹泻病毒；或The virus is an enveloped virus, such as African swine fever virus (ASFV), PRRS virus, pseudorabies virus, influenza virus, epidemic diarrhea virus; or

所述细菌选自沙门氏杆菌、大肠杆菌、金黄色葡萄球菌、空肠弯曲杆菌、梭菌。The bacteria are selected from Salmonella, Escherichia coli, Staphylococcus aureus, Campylobacter jejuni, Clostridium.