WO2020235917A1 - Composition for alleviating or treating acne, comprising, as active ingredient, extract of salvia plebeia r. br. or fraction thereof - Google Patents

Composition for alleviating or treating acne, comprising, as active ingredient, extract of salvia plebeia r. br. or fraction thereof Download PDF

Info

Publication number
WO2020235917A1
WO2020235917A1 PCT/KR2020/006549 KR2020006549W WO2020235917A1 WO 2020235917 A1 WO2020235917 A1 WO 2020235917A1 KR 2020006549 W KR2020006549 W KR 2020006549W WO 2020235917 A1 WO2020235917 A1 WO 2020235917A1
Authority
WO
WIPO (PCT)
Prior art keywords
fraction
acne
extract
active ingredient
composition
Prior art date
Application number
PCT/KR2020/006549
Other languages
French (fr)
Korean (ko)
Inventor
김동선
손은정
이윤미
육흥주
성윤영
Original Assignee
한국 한의학 연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국 한의학 연구원 filed Critical 한국 한의학 연구원
Publication of WO2020235917A1 publication Critical patent/WO2020235917A1/en

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/005—Preparations for sensitive skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Definitions

  • the present invention relates to a composition for improving or treating acne, comprising a gombo cabbage extract and a fraction thereof as an active ingredient.
  • acne is an inflammatory disease of the fur branch, and it is a very common skin disease that causes inflammation of the hair follicles on the skin of the face.
  • the cause of the occurrence of acne starts from the abnormal action of the androgen hormone, and the androgen hormone travels through the blood and flows into the sebaceous glands in the skin, promoting oil production, and the excessively secreted oil fills the pores of the sebum.
  • propionibacterium acnes which is an acne causative bacteria
  • a drug to which an antibiotic is added cannot be used as a cosmetic product.
  • research has been actively conducted to search for active ingredients that can reduce the side effects from natural resources, including plants, can be used continuously, and are harmless to the human body and have a strong anti-acne effect.
  • Gombo cabbage is an outpost of Salvia plebeia R. Br. belonging to the Lamiaceae family (Labiatae), and is also called Seolgyeoncho, Cheongwacho, Mamacho, Gwadongcheong, Suyangi, etc. flavonoids), homoplantaginin, hispidulin, eupafolin, eupafolin-7-glucoside, phenolic substances, essential oils, saponins, etc. This is known.
  • Korean Patent Publication No. 2016-0146007 discloses a composition for the treatment of respiratory inflammatory diseases comprising a specific crude extract of Gombo cabbage or a purified fraction isolated therefrom as an active ingredient
  • Korean Patent Publication No. 20111 No. -0078672 discloses a cosmetic composition containing an extract of pear-am chazugi as an active ingredient, but there is no disclosed composition for the improvement or treatment of acne comprising the extract of Gombo cabbage of the present invention and a fraction thereof as an active ingredient.
  • the present invention provides a composition for the improvement or treatment of acne comprising a gombo cabbage extract and a fraction thereof as an active ingredient, as derived from the above requirements, and the gombo cabbage extract of the present invention has no cytotoxicity, and the gombo cabbage By confirming that the extract and its fractions have excellent antibacterial activity against P. acnes , the present invention was completed.
  • the present invention provides a cosmetic composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the present invention provides a health functional food composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the present invention provides a pharmaceutical composition for the treatment of acne, comprising the extract of Gombo cabbage or a fraction thereof as an active ingredient.
  • the present invention provides a composition for antibacterial against Propionibacterium acnes comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the present invention relates to a composition for improving or treating acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient, wherein the gombo cabbage extract has excellent growth inhibition rate of propionibacterium acnes , an acne-causing bacteria Therefore, it can be usefully used in cosmetics for improving acne, pharmaceuticals for treating acne, or compositions for antibacterial.
  • Silymarin is a positive control.
  • Figure 2 is a photograph confirming that the propionibacterium acnes decreased according to the treatment of the Gombo cabbage extract of the present invention, the negative control group is not treated with the Gombo cabbage extract of the present invention.
  • BLK is a control without treatment. **, ***, **** mean to inhibit P. acnes statistically significantly compared to the control group (BLK), ** is p ⁇ 0.01, *** is p ⁇ 0.001, * *** is p ⁇ 0.0001.
  • Figure 4 is a result of confirming that the content of the chemokine RANTES decreased by the treatment of the extract of Gombo cabbage and its fractions of the present invention.
  • ### indicates that the content of RANTES was statistically significantly increased in the 10 ng/ml TNF- ⁇ and 10 ng/ml IFN- ⁇ -treated group (TI) compared to the control group, p ⁇ 0.001, * *, *** indicates that the content of RANTES in the gombo cabbage extract and fractions treated group of the present invention and the positive control silymarin treated group was statistically significantly reduced compared to the TI group, ** p ⁇ 0.01 And *** is p ⁇ 0.001.
  • the present invention relates to a cosmetic composition for improving acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the acne is preferably an acne caused by Propionibacterium acnes , but is not limited thereto.
  • the gombo cabbage extract may be prepared by a method including the following steps, but is not limited thereto.
  • step (3) preparing an extract by concentrating the filtered extract of step (2) under reduced pressure and drying it.
  • the extraction solvent in the step (1) is preferably water, a lower alcohol of C 1 to C 4 , or a mixture thereof, but is not limited thereto.
  • extraction of the gombo cabbage extract may be performed using any conventional method known in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction.
  • the extraction solvent is preferably extracted by adding 1 to 20 times the volume of the dried gombo cabbage, more preferably 3 to 10 times.
  • the extraction temperature is preferably 20 ⁇ 50 °C, but is not limited thereto.
  • the extraction time is preferably 10 to 100 hours, more preferably 24 to 96 hours, and most preferably 72 hours, but is not limited thereto.
  • the vacuum concentration in step (3) is preferably a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto.
  • the drying is preferably vacuum drying, vacuum drying, boiling drying, spray drying, or freeze drying, but is not limited thereto.
  • the fraction of the gombo cabbage extract is preferably a hexane fraction, an ethyl acetate fraction, a butanol fraction, or a water fraction, but is not limited thereto.
  • the cosmetic composition is a formulation selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. It is desirable to have, but is not limited thereto.
  • the cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.
  • the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Etc. may be used.
  • the formulation of the cosmetic composition of the present invention is a powder or a spray
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component.
  • chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.
  • a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
  • the formulation of the cosmetic composition of the present invention is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.
  • the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.
  • the cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobic substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, etc. .
  • the present invention relates to a health functional food composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the health functional food composition of the present invention may be prepared by adding the active ingredient, the gombo cabbage extract or a fraction thereof as it is, or mixing it with other foods or food ingredients, and may be appropriately prepared according to a conventional method.
  • foods to which the Gombo cabbage extract or its fractions can be added include caramel, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, and various soups.
  • Beverage, tea, drink, alcoholic beverage and vitamin complex may be in any one form selected from, and includes all health functional foods in the usual sense. That is, there is no particular limitation on the type of food.
  • the health functional food composition includes various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alkynic acid and salts thereof, organic acids, protective colloids It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. It may also contain pulp for the manufacture of natural fruit juices and vegetable beverages. The above components may be used independently or in combination.
  • the health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional components, and the natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and dextrin, Polysaccharides such as cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • the ratio of the natural carbohydrate is not very important, it is preferably 0.01 to 0.04 g, more preferably 0.02 to 0.03 g, but is not limited thereto with respect to 100 g of the composition of the present invention.
  • natural sweeteners such as taumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame can be used.
  • the present invention relates to a pharmaceutical composition for the treatment of acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • the pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent.
  • Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and include saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, lactose, and deck.
  • compositions of the present invention may be administered orally or parenterally, and parenteral administration may be administered by injection or application to the skin.
  • a suitable dosage of the pharmaceutical composition of the present invention can be variously prescribed depending on factors such as the method of formulation, mode of administration, age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the patient. I can.
  • the present invention relates to a composition for antibacterial against Propionibacterium acnes comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  • Solvent fractionation was performed using the obtained ethanol extract of Gombo cabbage. After 4.0 g of ethanol extract of Gombo cabbage was weighed and dissolved in 30 ml of water, 30 ml of hexane was added and mixed well, and the supernatant was repeated twice, and concentrated under reduced pressure to obtain a hexane fraction. Thereafter, 30 ml of ethyl acetate was added to the lower layer, mixed well, and the supernatant was taken and concentrated under reduced pressure to obtain an ethyl acetate fraction. After adding 20 ml of butanol again, mixing well, the supernatant was taken and concentrated under reduced pressure to obtain a butanol fraction, and a water fraction of the remaining lower layer was obtained.
  • CCK-8 Cell Counting Kit-8 assay was performed.
  • HaCaT cells were treated with samples at different concentrations (25, 50, 100, 200 ⁇ g/ml) and cultured for 24 hours. After adding CCK-8 at a concentration of 10%, reacting at 37° C. for 2 hours, and then measuring the absorbance at 450 nm using a microplate spectrophotometer (Biorad, Hercules, CA, USA) to calculate the cell viability (%). I did.
  • the amount of secretion of the chemokine RANTES was statistically significantly increased by the treatment (TI) of TNF- ⁇ and IFN- ⁇ , and the gombo cabbage extract of the present invention and its hexane (Hex) and When the ethyl acetate (EA) fraction was treated, it was confirmed that it was statistically significantly reduced.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Dermatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Polymers & Plastics (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Birds (AREA)
  • Medical Informatics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a composition for alleviating or treating acne, comprising, as an active ingredient, an extract of Salvia plebeia R. Br. or a fraction thereof, wherein the extract of Salvia plebeia R. Br. has an excellent growth inhibition rate of Propionibacterium acnes, which is acne-causing bacteria, thereby being effectively usable in cosmetics for alleviating acne, pharmaceuticals for treating acne or anti-bacterial compositions.

Description

곰보배추 추출물 및 이의 분획물을 유효성분으로 포함하는 여드름의 개선 또는 치료용 조성물Composition for improving or treating acne, comprising extract of Gombo cabbage and its fractions as active ingredients
본 발명은 곰보배추 추출물 및 이의 분획물을 유효성분으로 포함하는 여드름의 개선 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for improving or treating acne, comprising a gombo cabbage extract and a fraction thereof as an active ingredient.
일반적으로 여드름은 모피지선의 염증성 질환으로, 얼굴 피부 모낭에 염증을 일으키는 매우 흔한 피부 질환이다. 여드름 발생의 원인은 안드로겐 호르몬의 이상 작용에서부터 시작되는데, 안드로겐 호르몬이 혈액을 타고 피부에 있는 피지선으로 흘러들어가 오일 생산을 촉진시켜 과도하게 분비된 오일이 피지 모공 안을 가득 채우게 된다. 이때 오일의 과도한 생산과 더불어 모공 입구에서는 과각화 현상이 촉진되어 모공의 입구가 더욱 좁혀지거나 막히게 되는데, 이러한 현상이 모공 안을 가득 채우고 있는 오일들은 밖으로의 흐름을 멈추고 모공 안에 갇혀 정체되는데 이 갇힌 오일 덩어리들과 불순물들이 서로 엉켜 여드름 덩어리(Comedo)를 이루게 된다. 모공 안에 정체되어 있는 오일의 양이 많아지면, 박테리아인 프로피오니박테리움 아크네스( Propionibacterium acnes)가 활동하게 되고, 오일성분들을 탐식하고 난 후 생긴 부산물인 자유지방산이 모공을 심하게 자극하면서 염증을 유발시킨다. 일반적으로, 세균에 의한 여드름이나 염증과 같은 피부 질환의 치료에 있어서, 에리스로마이신(erythromycin), 테트라사이틀린(tetracycline), 신다마이신(cindamycine)과 같은 항균물질들이 주로 사용되어 왔으나, 이러한 항균물질은 장기간 사용시 여드름 원인균이 내성을 가지게 되고, 광 과민 작용과 같은 부작용을 일으키는 등의 많은 문제점을 야기해 왔다. In general, acne is an inflammatory disease of the fur branch, and it is a very common skin disease that causes inflammation of the hair follicles on the skin of the face. The cause of the occurrence of acne starts from the abnormal action of the androgen hormone, and the androgen hormone travels through the blood and flows into the sebaceous glands in the skin, promoting oil production, and the excessively secreted oil fills the pores of the sebum. At this time, excessive production of oil and hyperkeratosis is promoted at the pore opening, resulting in narrowing or clogging of the pore opening.This phenomenon stops the flow of oils filling the pores from the outside and is trapped in the pores, and these trapped oil lumps And impurities entangle with each other to form a acne lump (Comedo). When the amount of oil stagnant in the pores increases, the bacteria Propionibacterium acnes becomes active, and free fatty acids, a by-product after eating the oil components, severely irritate the pores and cause inflammation. Let it. In general, in the treatment of skin diseases such as acne or inflammation caused by bacteria, antibacterial substances such as erythromycin, tetracycline, and cindamycine have been mainly used, but these antibacterial substances are When used for a long period of time, acne causative bacteria become resistant and cause side effects such as photosensitization.
또한, 여드름 원인균인 프로피오니박테리움 아크네스는 인공적인 배양이 어렵고, 항생물질이 추가되는 약제는 화장품으로서의 이용이 불가능한 문제가 있다. 이에 대해, 최근에는 식물을 비롯한 천연자원으로부터 상기 부작용들을 줄이고 지속적으로 사용할 수 있으며 인체에 무해하면서도 강력한 항여드름균 효과를 갖는 유효성분들을 탐색하는 연구가 활발하게 진행되고 있다.In addition, propionibacterium acnes, which is an acne causative bacteria, is difficult to artificially culture, and a drug to which an antibiotic is added cannot be used as a cosmetic product. On the other hand, research has been actively conducted to search for active ingredients that can reduce the side effects from natural resources, including plants, can be used continuously, and are harmless to the human body and have a strong anti-acne effect.
한편, 곰보배추는 꿀풀과(Labiatae)에 속하는 배암차즈기(Salvia plebeia R. Br.)의 전초로서, 설견초, 청와초, 마마초, 과동청, 수양이 등으로도 불리며 성분으로는 플라보노이드 (flavonoids), 호모플란타기닌 (homoplantaginin), 히스피둘린 (hispidulin), 유파폴린 (eupafolin), 유파폴린-7-글루코시드 (eupafolin-7-glucoside) 외에도 페놀성 물질, 정유성분, 사포닌 등의 성분이 알려져 있다.On the other hand, Gombo cabbage is an outpost of Salvia plebeia R. Br. belonging to the Lamiaceae family (Labiatae), and is also called Seolgyeoncho, Cheongwacho, Mamacho, Gwadongcheong, Suyangi, etc. flavonoids), homoplantaginin, hispidulin, eupafolin, eupafolin-7-glucoside, phenolic substances, essential oils, saponins, etc. This is known.
곰보배추 관련 기술로는 한국공개특허 제2016-0146007호에 곰보배추 특정 조추출물 또는 이로부터 분리된 정제 분획물을 유효성분으로 포함하는 호흡기 염증 질환의 치료용 조성물이 개시되어 있고, 한국공개특허 제20111-0078672호에 배암차즈기 추출물을 유효성분으로 함유하는 화장료 조성물이 개시되어 있으나, 본 발명의 곰보배추 추출물 및 이의 분획물을 유효성분으로 포함하는 여드름의 개선 또는 치료용 조성물에 대해 개시된 바 없다.As a technology related to Gombo cabbage, Korean Patent Publication No. 2016-0146007 discloses a composition for the treatment of respiratory inflammatory diseases comprising a specific crude extract of Gombo cabbage or a purified fraction isolated therefrom as an active ingredient, and Korean Patent Publication No. 20111 No. -0078672 discloses a cosmetic composition containing an extract of pear-am chazugi as an active ingredient, but there is no disclosed composition for the improvement or treatment of acne comprising the extract of Gombo cabbage of the present invention and a fraction thereof as an active ingredient.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 곰보배추 추출물 및 이의 분획물을 유효성분으로 포함하는 여드름의 개선 또는 치료용 조성물을 제공하고, 본 발명의 곰보배추 추출물이 세포독성이 없으며, 곰보배추 추출물 및 이의 분획물이 P. acnes에 대한 항균활성이 우수하다는 것을 확인함으로써, 본 발명을 완성하였다.The present invention provides a composition for the improvement or treatment of acne comprising a gombo cabbage extract and a fraction thereof as an active ingredient, as derived from the above requirements, and the gombo cabbage extract of the present invention has no cytotoxicity, and the gombo cabbage By confirming that the extract and its fractions have excellent antibacterial activity against P. acnes , the present invention was completed.
상기 목적을 달성하기 위하여, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 건강기능식품 조성물을 제공한다.In addition, the present invention provides a health functional food composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 치료용 약학 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for the treatment of acne, comprising the extract of Gombo cabbage or a fraction thereof as an active ingredient.
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 프로피오니박테리움 아크네스( Propionibacterium acnes)에 대한 항균용 조성물을 제공한다.In addition, the present invention provides a composition for antibacterial against Propionibacterium acnes comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름의 개선 또는 치료용 조성물에 관한 것으로, 상기 곰보배추 추출물이 여드름 유발균인 프로피오니박테리움 아크네스( Propionibacterium acnes)의 생장 저해율이 우수하므로, 여드름 개선용 화장료, 여드름 치료용 의약품 또는 항균용 조성물에 유용하게 사용될 수 있다. The present invention relates to a composition for improving or treating acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient, wherein the gombo cabbage extract has excellent growth inhibition rate of propionibacterium acnes , an acne-causing bacteria Therefore, it can be usefully used in cosmetics for improving acne, pharmaceuticals for treating acne, or compositions for antibacterial.
도 1은 곰보배추 추출물의 세포독성을 확인한 결과이고, Silymarin은 양성대조군이다.1 is a result of confirming the cytotoxicity of the extract of Gombo cabbage, Silymarin is a positive control.
도 2는 본 발명의 곰보배추 추출물의 처리에 따른 프로피오니박테리움 아크네스가 감소한 것을 확인한 사진으로, 음성대조군은 본 발명의 곰보배추 추출물을 처리하지 않은 것이다.Figure 2 is a photograph confirming that the propionibacterium acnes decreased according to the treatment of the Gombo cabbage extract of the present invention, the negative control group is not treated with the Gombo cabbage extract of the present invention.
도 3은 곰보배추 분획물의 항균 활성을 확인한 것으로, Hex는 헥산 분획물이고, EA는 에틸아세테이트 분획물이며, BuOH은 부탄올 분획물이고, Water는 물 분획물이다. BLK는 아무것도 처리하지 않은 대조군이다. **, ***, ****은 대조군(BLK)에 비해 통계적으로 유의미하게 P. acnes을 저해하는 것을 의미하며, **는 p<0.01이고, ***는 p<0.001이며, ****는 p<0.0001이다.3 is a check of the antibacterial activity of the gombo cabbage fraction, where Hex is a hexane fraction, EA is an ethyl acetate fraction, BuOH is a butanol fraction, and Water is a water fraction. BLK is a control without treatment. **, ***, **** mean to inhibit P. acnes statistically significantly compared to the control group (BLK), ** is p<0.01, *** is p<0.001, * *** is p<0.0001.
도 4는 본 발명의 곰보배추 추출물 및 이의 분획물 처리에 의해 케모카인 RANTES 함량이 감소한 것을 확인한 결과이다. ###은 정상군(Control) 대비 10ng/㎖의 TNF-α와 10ng/㎖의 IFN-γ 처리군(TI)에서 RANTES의 함량이 통계적으로 유의미하게 증가하였다는 것으로, p<0.001이고, **, ***은 TI 군 대비 본 발명의 곰보배추 추출물 및 이의 분획물 처리군, 양성대조군인 실리마린(silymarin) 처리군의 RANTES의 함량이 통계적으로 유의미하게 감소하였다는 것으로, **은 p<0.01이며, ***은 p<0.001이다.Figure 4 is a result of confirming that the content of the chemokine RANTES decreased by the treatment of the extract of Gombo cabbage and its fractions of the present invention. ### indicates that the content of RANTES was statistically significantly increased in the 10 ng/ml TNF-α and 10 ng/ml IFN-γ-treated group (TI) compared to the control group, p<0.001, * *, *** indicates that the content of RANTES in the gombo cabbage extract and fractions treated group of the present invention and the positive control silymarin treated group was statistically significantly reduced compared to the TI group, ** p<0.01 And *** is p<0.001.
본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for improving acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
상기 여드름은 프로피오니박테리움 아크네스( Propionibacterium acnes)에 의해 유발된 여드름인 것이 바람직하지만 이에 한정하지 않는다. The acne is preferably an acne caused by Propionibacterium acnes , but is not limited thereto.
상기 곰보배추 추출물은 하기 단계를 포함하는 방법에 의해 제조될 수 있으나, 이에 한정하는 것은 아니다.The gombo cabbage extract may be prepared by a method including the following steps, but is not limited thereto.
(1) 곰보배추에 추출용매를 가하여 추출하는 단계;(1) extracting by adding an extraction solvent to Gombo cabbage;
(2) 상기 단계 (1)의 추출물을 여과하는 단계; 및(2) filtering the extract of step (1); And
(3) 상기 단계 (2)의 여과한 추출물을 감압 농축하고 건조하여 추출물을 제조하는 단계.(3) preparing an extract by concentrating the filtered extract of step (2) under reduced pressure and drying it.
상기 단계 (1)에서 추출용매는 물, C 1~C 4의 저급 알코올 또는 이들의 혼합물인 것이 바람직하지만 이에 한정하지 않는다. The extraction solvent in the step (1) is preferably water, a lower alcohol of C 1 to C 4 , or a mixture thereof, but is not limited thereto.
상기 제조방법에 있어서, 곰보배추 추출물의 추출은 여과법, 열수 추출, 침지 추출, 환류 냉각 추출 및 초음파추출 등 당업계에 공지된 모든 통상적인 방법을 이용할 수 있다. 상기 추출용매는 건조된 곰보배추 부피의 1~20배 첨가하여 추출하는 것이 바람직하며, 더 바람직하게는 3~10배 첨가하는 것이다. 추출온도는 20~50℃인 것이 바람직하나 이에 한정하지 않는다. 또한, 추출시간은 10~100시간인 것이 바람직하며, 24~96시간이 더욱 바람직하고, 72시간이 가장 바람직하나 이에 한정하지 않는다. 상기 방법에 있어서, 단계 (3)의 감압농축은 진공감압농축기 또는 진공회전증발기를 이용하는 것이 바람직하나 이에 한정하지 않는다. 또한, 건조는 감압건조, 진공건조, 비등건조, 분무건조 또는 동결건조하는 것이 바람직하나 이에 한정하지 않는다. 상기 곰보배추 추출물의 분획물은 헥산 분힉물, 에틸아세테이트 분획물, 부탄올 분획물 또는 물 분획물인 것이 바람직하지만 이에 한정하는 것은 아니다.In the above manufacturing method, extraction of the gombo cabbage extract may be performed using any conventional method known in the art such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction. The extraction solvent is preferably extracted by adding 1 to 20 times the volume of the dried gombo cabbage, more preferably 3 to 10 times. The extraction temperature is preferably 20 ~ 50 ℃, but is not limited thereto. In addition, the extraction time is preferably 10 to 100 hours, more preferably 24 to 96 hours, and most preferably 72 hours, but is not limited thereto. In the above method, the vacuum concentration in step (3) is preferably a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably vacuum drying, vacuum drying, boiling drying, spray drying, or freeze drying, but is not limited thereto. The fraction of the gombo cabbage extract is preferably a hexane fraction, an ethyl acetate fraction, a butanol fraction, or a water fraction, but is not limited thereto.
상기 화장료 조성물은 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 구성된 군으로부터 선택되는 제형을 갖는 것이 바람직하지만 이에 한정하는 것은 아니다. 이들 각 제형으로 이루어진 화장료 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.The cosmetic composition is a formulation selected from the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. It is desirable to have, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라가칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Etc. may be used.
본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판-부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
본 발명의 화장료 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.
본 발명의 화장료 조성물의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 아세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.
본 발명의 화장료 조성물은 형광물질, 살진균제, 굴수성 유발물질, 보습제, 방향제, 방향제 담체, 단백질, 용해화제, 당 유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobic substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, etc. .
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 건강기능식품 조성물에 관한 것이다.In addition, the present invention relates to a health functional food composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
본 발명의 건강기능식품 조성물은 상기 유효성분인 상기 곰보배추 추출물 또는 이의 분획물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 혼합하여 제조될 수 있고, 통상적인 방법에 따라 적절하게 제조될 수 있다. 상기 곰보배추 추출물 또는 이의 분획물을 첨가할 수 있는 식품의 예로는 카라멜, 육류, 소시지, 빵, 초콜릿, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 수프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 중에서 선택된 어느 하나의 형태일 수 있으며, 통상적인 의미에서의 건강기능식품을 모두 포함한다. 즉, 상기 식품의 종류에는 특별한 제한은 없다. 상기 건강기능식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 및 천연 풍미제, 착색제 및 증진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알킨산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 또한, 천연 과일 주스 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 상기의 성분은 독립적으로 또는 조합하여 사용할 수 있다. 또한, 본 발명의 건강기능식품 조성물은 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있으며, 상기 천연 탄수화물은 포도당, 과당과 같은 단당류, 말토스, 슈크로스와 같은 이당류, 및 덱스트린, 사이클로덱스트린과 같은 다당류, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 상기 천연 탄수화물의 비율은 크게 중요하지 않지만, 본 발명의 조성물 100g에 대하여, 0.01~0.04g 인 것이 바람직하고, 더욱 바람직하게는 0.02~0.03g을 포함하는 것이지만 이에 한정하지 않는다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. The health functional food composition of the present invention may be prepared by adding the active ingredient, the gombo cabbage extract or a fraction thereof as it is, or mixing it with other foods or food ingredients, and may be appropriately prepared according to a conventional method. Examples of foods to which the Gombo cabbage extract or its fractions can be added include caramel, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, and various soups. , Beverage, tea, drink, alcoholic beverage and vitamin complex may be in any one form selected from, and includes all health functional foods in the usual sense. That is, there is no particular limitation on the type of food. The health functional food composition includes various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alkynic acid and salts thereof, organic acids, protective colloids It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. It may also contain pulp for the manufacture of natural fruit juices and vegetable beverages. The above components may be used independently or in combination. In addition, the health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional components, and the natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and dextrin, Polysaccharides such as cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. Although the ratio of the natural carbohydrate is not very important, it is preferably 0.01 to 0.04 g, more preferably 0.02 to 0.03 g, but is not limited thereto with respect to 100 g of the composition of the present invention. As the sweetener, natural sweeteners such as taumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame can be used.
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 치료용 약학 조성물에 관한 것이다.In addition, the present invention relates to a pharmaceutical composition for the treatment of acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
본 발명의 약학 조성물은 약학적으로 허용되는 담체, 부형제 또는 희석제를 추가로 포함할 수 있다. 본 발명의 약학 조성물에 포함되는 약학적으로 허용되는 담체는 제제 시에 통상적으로 이용되는 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 덱스트로즈 용액, 말토덱스트린 용액, 글리세롤, 에탄올, 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산 칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세결정성 셀룰로스, 폴리비닐피롤리돈, 셀룰로스, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and include saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, lactose, and deck. Straw, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methyl cellulose, methyl hydroxybenzoate, propyl Hydroxybenzoate, talc, magnesium stearate, mineral oil, and the like, but are not limited thereto.
또한 상기 유효성분 이외에 항산화제, 완충액, 정균제, 희석제, 계면활성제, 결합제, 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제 또는 보존제 등을 추가로 포함할 수 있다. 본 발명의 약학 조성물은 경구 또는 비경구 투여할 수 있으며, 비경구 투여는 주사 또는 피부에 도포하는 방법으로 투여할 수 있는 것이다. 본 발명의 약학 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하게 처방될 수 있다. In addition, antioxidants, buffers, bacteriostatic agents, diluents, surfactants, binders, lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents or preservatives, etc. may be further included in addition to the active ingredients. The pharmaceutical composition of the present invention may be administered orally or parenterally, and parenteral administration may be administered by injection or application to the skin. A suitable dosage of the pharmaceutical composition of the present invention can be variously prescribed depending on factors such as the method of formulation, mode of administration, age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the patient. I can.
또한, 본 발명은 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 프로피오니박테리움 아크네스( Propionibacterium acnes)에 대한 항균용 조성물에 관한 것이다.In addition, the present invention relates to a composition for antibacterial against Propionibacterium acnes comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
이하, 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다. Hereinafter, the present invention will be described in more detail using examples. These examples are only for describing the present invention in more detail, and it is apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.
실시예 1. 곰보배추 추출물 및 분획물의 제조Example 1. Preparation of extract and fraction of Gombo cabbage
480g의 곰보배추 원물에 대하여, 70%(v/v) 에탄올을 720㎖ 가한 후, 3시간 환류 추출하고, 이를 감압 농축하여 동결건조하여, 140.6g의 곰보배추 추출물을 획득하였다. To 480 g of the original Gombo cabbage, 720 ml of 70% (v/v) ethanol was added, followed by extraction under reflux for 3 hours, concentrated under reduced pressure and lyophilized to obtain 140.6 g of Gombo cabbage extract.
상기 획득한 곰보배추 70% 에탄올 추출물을 이용하여 용매 분획을 실시하였다. 곰보배추 에탄올 추출물 4.0g을 칭량하여 물 30㎖에 용해한 후, 동량의 헥산 30㎖을 가하여 잘 혼합하고 상층액을 취하기를 2회 반복한 후, 감압농축하여 헥산 분획물을 획득하였다. 이후 하층액에 에틸아세테이트 30㎖을 가하여 잘 혼합한 후 상층액을 취하여 감압농축하여 에틸아세테이트 분획물을 획득하였다. 다시 부탄올 20㎖을 가하여 잘 섞어준 후 상층액을 취하여 감압농축하여 부탄올 분획물을 획득하였고, 나머지 하층의 물 분획물을 획득하였다.Solvent fractionation was performed using the obtained ethanol extract of Gombo cabbage. After 4.0 g of ethanol extract of Gombo cabbage was weighed and dissolved in 30 ml of water, 30 ml of hexane was added and mixed well, and the supernatant was repeated twice, and concentrated under reduced pressure to obtain a hexane fraction. Thereafter, 30 ml of ethyl acetate was added to the lower layer, mixed well, and the supernatant was taken and concentrated under reduced pressure to obtain an ethyl acetate fraction. After adding 20 ml of butanol again, mixing well, the supernatant was taken and concentrated under reduced pressure to obtain a butanol fraction, and a water fraction of the remaining lower layer was obtained.
이와 같이 획득한 각각의 분획물의 수율은 표 1에 개시하였다.The yield of each fraction thus obtained is disclosed in Table 1.
수득양(mg)Yield (mg) 수율(%)yield(%)
헥산Hexane 139.6139.6 3.503.50
에틸아세테이트Ethyl acetate 339.9339.9 8.508.50
부탄올Butanol 404.4404.4 10.1110.11
물water 2731.42731.4 68.2968.29
실시예 2. 곰보배추 추출물의 세포독성 확인Example 2. Confirmation of Cytotoxicity of Gombo Cabbage Extract
곰보배추 추출물의 인간 각질세포(human keratinocyte) HaCaT 세포독성을 확인하기 위하여, CCK-8(Cell Counting Kit-8) 어세이를 실시하였다. 96웰 플레이트에서 HaCaT 세포에 시료를 농도별(25, 50, 100, 200㎍/㎖)로 처리하고 24시간 동안 배양하였다. CCK-8을 10% 농도로 첨가한 다음 2시간 동안 37℃에서 반응시킨 후, 마이크로플레이트 분광광도계(Biorad, Hercules, CA, USA)를 이용하여 450nm에서 흡광도를 측정하여 세포 생존율(%)을 계산하였다. In order to confirm the cytotoxicity of human keratinocyte HaCaT of the extract of Gombo cabbage, CCK-8 (Cell Counting Kit-8) assay was performed. In a 96-well plate, HaCaT cells were treated with samples at different concentrations (25, 50, 100, 200 µg/ml) and cultured for 24 hours. After adding CCK-8 at a concentration of 10%, reacting at 37° C. for 2 hours, and then measuring the absorbance at 450 nm using a microplate spectrophotometer (Biorad, Hercules, CA, USA) to calculate the cell viability (%). I did.
그 결과, 도 1에 개시한 바와 같이, 곰보배추 추출물은 HaCaT 세포독성이 거의 없는 것으로 나타났다.As a result, as disclosed in Figure 1, it was found that the extract of Gombo cabbage had little HaCaT cytotoxicity.
실시예 3. 곰보배추 추출물 및 분획물의 처리에 따른 여드름균의 생장 저해율 확인Example 3. Confirmation of growth inhibition rate of acne bacteria according to treatment of extracts and fractions of Gombo cabbage
여드름균 P. acnes KCTC 3314를 사용하였으며, Reinforced Clostridial medium(Difco, USA)을 혐기조건에서 실험하기 위하여, RC 액체배지에서 48시간 동안 전배양한 후, 1.5×10 8 CFU/㎖의 농도로 희석한 배지에 시료 용액을 첨가하여 37℃에서 48시간 동안 혐기조건에서 여드름균 P. acnes의 균 감소율(%)을 확인하였다. 균 감소율은 600nm에서 흡광도 측정을 통해 확인하였다.Acne bacteria P. acne s KCTC 3314 was used, and Reinforced Clostridial medium (Difco, USA) was pre-cultured in RC liquid medium for 48 hours to experiment under anaerobic conditions, and then at a concentration of 1.5×10 8 CFU/ml. The sample solution was added to the diluted medium, and the reduction rate (%) of acne bacteria P. acnes was confirmed under anaerobic conditions at 37°C for 48 hours. The bacteria reduction rate was confirmed by measuring the absorbance at 600 nm.
그 결과 도 2 및 표 2에 개시한 바와 같이, 곰보배추 추출물 500㎍/㎖에서 98.5±0.28%의 항균효과를 나타냈고, 도 3에 개시한 바와 같이 헥산 분획물, 에틸아세테이트 분획물에서 여드름균인 P. acnes의 균에 대한 저해활성이 강하게 나타나는 것을 확인하였다.As a result, as disclosed in FIGS. 2 and 2, the antibacterial effect of 98.5±0.28% was exhibited in 500 μg/ml of Gombo cabbage extract, and as disclosed in FIG. 3, the acne bacteria P in the hexane fraction and ethyl acetate fraction It was confirmed that the inhibitory activity of acnes against bacteria was strongly exhibited.
여드름균 P. acnes KCTC 3314Acne bacteria P. acnes KCTC 3314 저해활성(%)Inhibitory activity (%) 표준편차Standard Deviation
곰보배추 추출물Gombo cabbage extract 500㎍/㎖500㎍/㎖ 98.598.5 0.280.28
250㎍/㎖250㎍/㎖ 75.875.8 0.10.1
100㎍/㎖100㎍/㎖ 7.97.9 2.42.4
실시예 4. 케모카인 분비 억제 효능 실험Example 4. Experiment of chemokine secretion inhibition efficacy
배양된 인간 각질 세포(keratinocyte)를 6-웰 플레이트(well plate)에 접종하여 시료를 농도별 25, 50㎍/㎖로 1시간 전처리한 후, 10ng/㎖의 TNF-α와 10ng/㎖의 IFN-γ를 처리하고 24시간 동안 배양하였다. 양성대조군으로 silymarin(12, 25㎍/㎖)을 각각 처리하였다. 24시간 후에 배지에 분비된 케모카인 RANTES의 분비량을 ELISA kit (R&D systems Inc., Minneapolis, MN, USA)로 측정하였다. Cultured human keratinocytes were inoculated into a 6-well plate, and samples were pretreated at 25 and 50 µg/ml for 1 hour, followed by 10 ng/ml TNF-α and 10 ng/ml IFN. -γ was treated and incubated for 24 hours. Silymarin (12, 25㎍/㎖) was treated as a positive control, respectively. After 24 hours, the amount of secretion of the chemokine RANTES secreted into the medium was measured with an ELISA kit (R&D systems Inc., Minneapolis, MN, USA).
모든 측정 결과는 평균(mean)과 표준편차(standard deviation; SD)로 나타내었으며, 실험군 간의 차이는 Student's t-test를 사용하여 통계학적 분석을 수행하였고, p < 0.05 값인 경우에 통계적으로 유의성이 있는 것으로 판정하였다.All measurement results were expressed as mean and standard deviation (SD), and the difference between the experimental groups was statistically analyzed using Student's t-test, and statistically significant when p <0.05. It was determined to be.
그 결과, 도 4에 개시한 바와 같이, 케모카인 RANTES의 분비량이 TNF-α 및 IFN-γ의 처리(TI)에 의해 통계적으로 유의하게 증가하였고, 본 발명의 곰보배추 추출물 및 이의 헥산(Hex) 및 에틸아세테이트(EA) 분획물을 처리한 경우, 통계적으로 유의미하게 감소한 것을 확인하였다. As a result, as disclosed in FIG. 4, the amount of secretion of the chemokine RANTES was statistically significantly increased by the treatment (TI) of TNF-α and IFN-γ, and the gombo cabbage extract of the present invention and its hexane (Hex) and When the ethyl acetate (EA) fraction was treated, it was confirmed that it was statistically significantly reduced.

Claims (8)

  1. 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 화장료 조성물.A cosmetic composition for improving acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  2. 제1항에 있어서, 상기 여드름은 프로피오니박테리움 아크네스( Propionibacterium acnes)에 의해 유발된 여드름인 것을 특징으로 하는 여드름 개선용 화장료 조성물.The cosmetic composition for improving acne according to claim 1, wherein the acne is acne caused by Propionibacterium acnes .
  3. 제1항에 있어서, 상기 곰보배추 추출물의 용매는 물, C 1~C 4의 저급알코올 또는 이들의 혼합물인 것을 특징으로 하는 여드름의 개선용 화장료 조성물.The cosmetic composition for improving acne according to claim 1, wherein the solvent of the gombo cabbage extract is water, a lower alcohol of C 1 to C 4 or a mixture thereof.
  4. 제1항에 있어서, 상기 곰보배추 추출물의 분획물은 헥산 분획물 또는 에틸아세테이트 분획물인 것을 특징으로 하는 여드름 개선용 화장료 조성물.The cosmetic composition for improving acne according to claim 1, wherein the fraction of the gombo cabbage extract is a hexane fraction or an ethyl acetate fraction.
  5. 제1항에 있어서, 상기 화장료 조성물은 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 구성된 군으로부터 선택되는 제형을 갖는 것을 특징으로 하는 여드름 개선용 화장료 조성물.The method of claim 1, wherein the cosmetic composition is composed of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray. A cosmetic composition for improving acne, characterized in that it has a formulation selected from the group.
  6. 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 개선용 건강기능식품 조성물.A health functional food composition for improving acne, comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  7. 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 여드름 치료용 약학 조성물.A pharmaceutical composition for the treatment of acne comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
  8. 곰보배추 추출물 또는 이의 분획물을 유효성분으로 포함하는 프로피오니박테리움 아크네스( Propionibacterium acnes)에 대한 항균용 조성물.An antibacterial composition for propionibacterium acnes comprising a gombo cabbage extract or a fraction thereof as an active ingredient.
PCT/KR2020/006549 2019-05-21 2020-05-19 Composition for alleviating or treating acne, comprising, as active ingredient, extract of salvia plebeia r. br. or fraction thereof WO2020235917A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020190059617A KR102070789B1 (en) 2019-05-21 2019-05-21 Composition for ameliorating or treating acne comprising Salvia plebeia extract and its fraction as effective component
KR10-2019-0059617 2019-05-21

Publications (1)

Publication Number Publication Date
WO2020235917A1 true WO2020235917A1 (en) 2020-11-26

Family

ID=69322434

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/006549 WO2020235917A1 (en) 2019-05-21 2020-05-19 Composition for alleviating or treating acne, comprising, as active ingredient, extract of salvia plebeia r. br. or fraction thereof

Country Status (2)

Country Link
KR (1) KR102070789B1 (en)
WO (1) WO2020235917A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102070789B1 (en) * 2019-05-21 2020-01-29 한국 한의학 연구원 Composition for ameliorating or treating acne comprising Salvia plebeia extract and its fraction as effective component
KR102161942B1 (en) * 2020-07-27 2020-10-05 이훈 Anti-acne cosmetic composition and method for manufacturing the same

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110138984A (en) * 2010-06-22 2011-12-28 배승강 Cosmetic composition for improving skin using gombo-baechu and manufacturing method thereof
JP2013067606A (en) * 2011-09-08 2013-04-18 Kao Corp ESTROGEN RECEPTOR β ACTIVATOR
CN105412340A (en) * 2015-12-11 2016-03-23 郑州郑先医药科技有限公司 Traditional Chinese medicine preparation for treating acne and preparation method thereof
KR20160130624A (en) * 2015-05-04 2016-11-14 재단법인 경기과학기술진흥원 A Pharmaceutical Composition Comprising Extract or Fraction of Salvia plebeia R. Br. for Preventing or Treating Gout
CN106668516A (en) * 2015-11-06 2017-05-17 广州青岚生物科技有限公司 Composition of drug extract for eliminating face acne marks and preparation method of composition
KR102070789B1 (en) * 2019-05-21 2020-01-29 한국 한의학 연구원 Composition for ameliorating or treating acne comprising Salvia plebeia extract and its fraction as effective component

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110138984A (en) * 2010-06-22 2011-12-28 배승강 Cosmetic composition for improving skin using gombo-baechu and manufacturing method thereof
JP2013067606A (en) * 2011-09-08 2013-04-18 Kao Corp ESTROGEN RECEPTOR β ACTIVATOR
KR20160130624A (en) * 2015-05-04 2016-11-14 재단법인 경기과학기술진흥원 A Pharmaceutical Composition Comprising Extract or Fraction of Salvia plebeia R. Br. for Preventing or Treating Gout
CN106668516A (en) * 2015-11-06 2017-05-17 广州青岚生物科技有限公司 Composition of drug extract for eliminating face acne marks and preparation method of composition
CN105412340A (en) * 2015-12-11 2016-03-23 郑州郑先医药科技有限公司 Traditional Chinese medicine preparation for treating acne and preparation method thereof
KR102070789B1 (en) * 2019-05-21 2020-01-29 한국 한의학 연구원 Composition for ameliorating or treating acne comprising Salvia plebeia extract and its fraction as effective component

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HUANG, C.: "Antimicrobial herbs against Staphylococcus aureusa and Propionibacterium acnes", PLANTA. MED., vol. 76, no. 12, 2010, pages 500, XP055762876 *

Also Published As

Publication number Publication date
KR102070789B1 (en) 2020-01-29

Similar Documents

Publication Publication Date Title
KR20190079202A (en) Fermentation method of lactic acid bacteria using citrus fruit and its use
WO2020235917A1 (en) Composition for alleviating or treating acne, comprising, as active ingredient, extract of salvia plebeia r. br. or fraction thereof
KR101125224B1 (en) A composition for skin anti-aging comprising extracts or fractions of Eremochloa ophiuroides as an active ingredient
KR101125225B1 (en) A composition for skin wrinkle improvement comprising extracts or fractions of Eremochloa ophiuroides as an active ingredient
KR101800962B1 (en) Composition for Improving Skin Wrinkles Using an Extract of Reed or a Product Obtained by Fermenting the Same with Lactic Acid Bacteria
KR102094949B1 (en) Whitening composition containing extract, fractions or compound derived from Raphanus sativus L. var niger
KR102453568B1 (en) Composition for moisturizing and anti-inflammation comprising fermented Codium fragile extract fermented by inoculating Bacillus safensis strain
WO2020180139A2 (en) Composition, for preventing, relieving and treating atopic dermatitis, comprising as active ingredient gardenia fruit extract from which pigments are removed
KR102226131B1 (en) Composition for skin moisturizing comprising extract of fermented Setaria italica as effective component
KR102231474B1 (en) Composition for preventing, ameliorating or treating acne comprising withaferin A as effective component
KR102283946B1 (en) Anti-inflammation Compound derived from Yuja and Isolating Method Thereof
KR101721666B1 (en) Skin-whitening compound from Inula britannica, and skin-whitening composition containing the same
KR101877413B1 (en) Composition containing Melandrii Herba extract reducing lipid accumulation as effective component
KR101862135B1 (en) Novel diynoic acid compound and pharmaceutical composition for preventing or treating bone diseases comprising the same
WO2018186705A1 (en) Composition, containing siraitia grosvenorii residue extract as active ingredient, for prevention, alleviation, or treatment of inflammation
KR20200089527A (en) Composition comprising extracts of Acorus gramineus and Dendropanax morbifera for anti-inflammation
KR102600557B1 (en) A composition having anti-inflammation activity comprising compounds isolated from the fraction of the Podocarpus macrophyllus extracts as an active ingredient
KR102694743B1 (en) Anti-microbial composition comprising biorenovation extract of Lycium chinense sprout as effective component
WO2024117771A1 (en) Composition including colored rice extract as active ingredient for prevention, reduction, or treatment of skin wrinkles
KR20200069616A (en) Anti-inflammatory composition comprising Prunus pendula for. ascendens (Makino) Ohwi
KR102445679B1 (en) Composition of extracts for antioxidant property containing silk worm pupae and red ginseng
KR102563733B1 (en) Composition for wound treatment or skin regeneration containing Aloe vera flower extract as active ingredients
WO2023058993A1 (en) Composition for anti-aging and skin improvement, comprising complex of indian gooseberry extract and barley sprout extract (ib complex) as active ingredient
KR20180060169A (en) Composition for Preventing or Improving Atopic dermatitis Comprising Extract of Galium Aparine as Effective Component
WO2020091232A1 (en) Composition for prevention, improvement or treatment of atopic dermatitis, comprising lumbricus rubellus extract or fraction thereof as active ingredient

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20809345

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20809345

Country of ref document: EP

Kind code of ref document: A1