WO2020206518A1 - Composições biológicas de funções múltiplas - Google Patents

Composições biológicas de funções múltiplas Download PDF

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Publication number
WO2020206518A1
WO2020206518A1 PCT/BR2019/050236 BR2019050236W WO2020206518A1 WO 2020206518 A1 WO2020206518 A1 WO 2020206518A1 BR 2019050236 W BR2019050236 W BR 2019050236W WO 2020206518 A1 WO2020206518 A1 WO 2020206518A1
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WIPO (PCT)
Prior art keywords
methyl
carboxamide
bacillus
pyrazole
spp
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PCT/BR2019/050236
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English (en)
French (fr)
Inventor
Eduardo Roberto de Almeida BERNARDO
Thales Facanali MARTINS
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Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda
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Application filed by Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda filed Critical Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda
Priority to US17/425,004 priority Critical patent/US20220087260A1/en
Priority to EP19924576.2A priority patent/EP3954214A4/en
Publication of WO2020206518A1 publication Critical patent/WO2020206518A1/pt

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • compositions of multiple functions for the biological control of phytopathogens, phytomatodes, agricultural pests, inoculant, phosphorus solubilization, soil conditioning, biostimulant, water stress reduction in cultivated plants and bioremediation in arable or non-arable areas are compositions containing mixtures of additives and excipients to at least 2 (two) species of Bacillus or 2 (two) or more strains of the same species and their mutants; at least 2 (two) representatives of fungi of the orders Hypocreales, Orbiliales, Helotiales and Mucorales or 2 (two) or more strains of the same species of representatives of the orders and their mutants; Bacillus in mixtures with fungi of the orders Hypocreales, Orbiliales, Helotiales and Mucorales, and combination of them or part of them (in colony-forming units, ufc) together or not with agricultural pesticides (fungicides, insecticides, acaricides, herbicide
  • antagonism and antibiosis tend to be the predominant mode of action of bacteria against phytomatodes and fungi. In the case of fungi, competition and parasitism are the most present.
  • these nematicidal compounds can have a direct action on the hatching of eggs or on mobility, and indirect effects, such as alteration of root exudates, confusion in locomotion or induction of resistance are also observed (Sikora & Hoffmann-Hergarten , 1992; Hasky-Ginther et al. 1998).
  • the use of bacteria as agents of biological control has shown to be a promising alternative in the productive system (Hallmann et al.
  • the genus Bacillus spp. is recognized as an excellent producer of antimicrobial compounds (Huang & Chang, 1975; Baker et al., 1983; Motomura & Hirooka, 1996) and the finding of the production of phytopathogen-inhibiting substances, produced by several isolates of Bacillus spp., is especially important for understanding the mechanisms of action of these microorganisms for antagonism.
  • Antagonistic bacteria like B. subtilis, generally act significantly by antibiosis and, occasionally, by parasitism and competition.
  • the control of the disease can be effective due to the substances produced and, not only due to the mass introduction of biocontrol agents (Arras & Arru, 1997).
  • Microorganisms that act by antibiosis generally have a broad spectrum of action, so that in the inhibition of fungi the production of toxic substances is more effective than any other mechanism of action involved.
  • RPCP plant growth promoting rhizobacteria
  • the objective of the present invention is to provide effective compositions for the reduction of damages caused by phytopathogens, phytomatodes, agricultural pests, to promote plant growth, to condition soils and to mitigate damages caused by eventual water stresses or environmental contamination.
  • the present invention relates to multi-purpose biological compositions, for spraying, bar spraying, aerial spraying, low volume, ultra low volume, planting furrow spraying, drench, coating (industrial or not), treatment (industrial or not) and incrustation (industrial or not) of seeds of cultivated plants, containing mixtures of at least 2 (two) species of Bacillus or 2 (two) or more strains of the same species and their mutants; at least 2 (two) representatives of fungi of the orders Hypocreales, Orbiliales, Helotiales and Mucorales and their mutants or 2 (two) or more strains of the same species of representatives of the orders; Bacillus in mixtures with fungi of the orders Hypocreales, Orbiliales, Helotiales and Mucorales, and combination of them or part of them (in colony-forming units, ufc) together or not with agricultural pesticides (fungicides, insecticides, acaricides, herbicides and nematicides), plant essential oils or plant extracts
  • compositions of multiple functions comprise: 1.0 to 90.0% of at least 2 (two) species of Bacillus or 2 (two) or more strains of the same species and their mutants;
  • Bacillus that comprise the order Bacilales and their mutants belong to the group consisting of Bacillus velezensis, Bacillus aryabhattai, Bacillus genus, Bacillus pseudomycoides, Bacillus mucilaginosus, Bacillus sonorensis, Bacillus agri, Bacillus aizawai, Bacillus firmis, Bacillus firmis coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nichillis, Bacillus nichillis, Bacillus nichillis subtilis, Bacilus licheniformis, Bacilus paralicheniformis, Bacill
  • Fungi and their mutants belong to the group comprising Hypocreales, not restricting to the genera and species of Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, brevicompactum Trichoderma, Trichoderma cinnamomeum, hamatum Trichoderma, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, virens Trichoderma, Trichoderma viride, viridescens Trichoderma Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora,
  • the fungi and their mutants belong to the group that comprises the order Orbiliales, not restricting to the genera and species of Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotryso constructions, Arthrobotryso , Arthrobotrys dactyloides, Arthrobotrys elegans Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Irregular Arthrobotrys, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora, Dactylaria haptotyla, Dactylaria.
  • Fungi and their mutants belong to the group comprising the order Helotiales, not restricting to the genera and species of Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum, Monacrosporium fusiforme, Monacrosporium globosporium, Monacrosporium, Monacrosporum, Monacrosporum, Dactylella aphrobrocha, Dactylella arcuata, Dactylella arnaudii, Dactylella atractoides, Dactylella attenuata, Dactylella candida, Dactylella cionopaga, Dactylella fusiformis, Dactylella heterospora, Dactylella heterospora or any other genus Dactylella branches, Dactylella heterospora.
  • Fungi and their mutants belong to the group that comprises the order Mucorales, not restricting to the genera and species of Cunninghamella elegans and any other genus or species of the aforementioned order.
  • Pesticides consist of:
  • Fungicides benzovindiflupyr, antiperonosporic, ametoctradin, amisulbrom, copper hydroxide, copper oxychloride, copper, sulfate, copper persulfate, boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, flux, ampronil, isofetam, fenproxy, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyrametostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, fentin hydroxide, oxytetracycline, chlozolinate, chloroneb, tecnazene, e
  • Herbicides alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; phenoxyphenoxypropionic ester, clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, phenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop-butyl, fluazifop-P-butyl, haloxyfop-ethoxyopyl, haloxyop -methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl or quizalofop-tefuryl, flamprop-methyl
  • esprocarb molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, thiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butififen, acylif, acid, chifort, ac , bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl,
  • Nematicides Benomyl, cloethocarb, aldoxycarb, tirpate, diamidafos, fenamiphos, cadusafos, dichlofenthion, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofof, isazofos, phosphocarb, thionazin, imicyafz, agicy, ze fluensulfone, 1,3-dichloropropene (telone), dimethyl disulfide, metam sodium, metam potassium, methyl bromide, allyl isothiocyanate (AITC), dimethyl sulfate, furfual (aldehyde), among others.
  • AITC allyl isothiocyanate
  • Essential oils and plant extracts obtained from seeds, plants and part of plants belong to the group consisting of: Ricinus communis, Crotalaria juncea, Chenopodium ambrosioides, Azadirachta indica, Verbena officinalis, Erythrina mulungu, Quassia amara, Bidens pilosa, Plantago lanceolata, Senecio brasiliensis, Lantana chamber, Tagetes patula, Lantana montevidensis, Eucalyptus spp., Lantana montevidensis, Lantana chamber, Senecio brasiliensis, Chrysantemum coronarium, Solanum americanum, Solanum paniculatum Hovenia dulcisia, Melia, Azia, Melia , Conyza bonariensis, Senecio brasiliensis, Bidens pilosa, Amaranthus hybridus, Euphorbia
  • the additives are dispersants selected from the group consisting of dispersants selected from the group consisting of water-soluble ionic polymers, amino acids, algae extracts, compounds of natural origin, water-soluble anionic polymers, surfactants selected from the group consisting of anionic surfactants and nonionic surfactants and combinations of them and derivatives.
  • the excipients belong to the group consisting of: silicas, talc, bentonite, carbohydrates, sugars, starches, carbonates, casein, whey and milk derivatives and combinations between them and derivatives.
  • compositions can be used as an encapsulated suspension formulation, dispersible concentrate, emulsifiable concentrate, water-in-oil emulsion, oil-in-water emulsion, microemulsion, concentrated suspension, suspo-emulsion, soluble granulate, soluble concentrate, soluble powder , tablet, dispersible granules, wettable powder, emulsifiable gel, water-soluble gel, emulsifiable granule, emulsifiable powder, oil dispersion or oil-concentrated suspension, mixed formulation of soluble concentrate and concentrated suspension, mixed formulation of soluble concentrate and suspo-emulsion , mixed formulation of soluble concentrate and oil-in-water emulsion, dispersible or oil-miscible concentrated suspension, oil-miscible solution, oil-dispersible powder, encapsulated granules, dry powder, electrostatic / electrodynamic spray liquid, granules, spray oil / spreading, ultra low volume suspension,
  • Biological compositions of multiple functions are for the biological control of phytopathogens, phytomatodes and agricultural pests, inoculant, phosphorus solubilization, soil conditioning, biostimulant, water stress reduction in cultivated plants and bioremediation in agricultural areas or not with nematicidal effects in the reduction of damages and control of phytenomatodes, not limited to Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incognita, Meloidogyne javanica, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Meloidogyne exigua, Helicotilechus dihystera, Meloidogyne exigua, Meloidogyne paranaensis, Meloidogyne coffeicola, Meloidogyne hapla, Meloidogyne enterolobii,
  • Nicotiana tabacum, ornamental, fruit (Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus persisica, Passiflora edulis), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidiaria, Brachiaria humidiaria, Brachiaria humidiaria , Brachiaria arrecta, Brachiaria dictyneura), Panicum spp.
  • Bacillus licheniformis 1.0 x 10 9 cfu / g
  • Bacillus subtilis 1.0 x 10 9 cfu / g
  • Trichoderma harzianum 1.0 x 10 7 cfu / g
  • the composition may have the following concentrations:
  • Bacillus amyloliquefasciens (strain CPQBA 040-11 DRM 01): 1.0 to 20.0% Bacillus amyloliquefasciens (strain CPQBA 040-11 DRM 04): 1.0 to 20.0% Additives: 1.0 to 20.0%
  • Bacillus amyloliquefasciens 1.0 to 20.0%
  • Bacillus licheniformis 1.0 to 20.0%
  • Bacillus subtilis 1.0 to 20.0%
  • Bacillus amyloliquefaciens 1.0 to 20.0% Trichoderma harzianum: 1.0 to 20.0% Azoxistrobin 5.0 to 10.0% Additives: 1.0 to 20.0%
  • Bacillus thuringiensis 10.0 to 20.0% Aschersonia aleyrodis: 10.0 to 20.0%
  • Aschersonia aleyrodis 10.0 to 20.0%
  • Aschersonia aleyrodis 10.0 to 20.0%
  • a composition containing 10.0% Bacillus amyloliquefasciens (strain CPQBA 040-11 DRM 01), 10.0% Bacillus amyloliquefasciens (strain CPQBA 040-11 DRM 04), 3% acrylic styrene polymer, 2, 0% anionic surfactant and 75.0% inert was formulated to evaluate its efficiency in the control of phytopathogens, biostimulation, growth promotion and soil conditioning.
  • a composition containing 10.0% Bacillus subtilis, 10.0% Bacillus amyloliquefasciens, 10.0% Malaleuca quinquenervia oil, 6.0% anionic surfactant and 64.0% inert was formulated to evaluate its efficiency in the control of phytopathogens.
  • a composition containing 10.0% Bacillus amyloliquefasciens, 8.0% Bacillus licheniformis, 10% Trichoderma harzianum 18.0% Azoxistrobin, 7.5% anionic surfactant, and 46.5% inert was formulated to assess its efficiency in phytopathogen control, nematode control, growth promotion and soil conditioning.
  • a composition containing 15.0% Beauveria bassiana, 7.0% Bacillus thuringiensis, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 51.5 % of inert was formulated to evaluate its efficiency in insect control.
  • a composition containing 15.0% Beauveria bassiana, 15.0% Isaria fumosorosea, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 43.5 % of inert was formulated to evaluate its efficiency in insect control.
  • a composition containing 15.0% Beauveria bassiana, 20.0% Aschersonia aleyrodis, 4.0% acrylic styrene polymer, 2.5% anionic surfactant and 58.5% inert was formulated to evaluate its efficiency in insect control.
  • compositions for the biological control of phytopathogens, phytomatodes and insects, inoculants, soil conditioners, biostimulants, water stress reducers in plants and bioremediation should follow the following flow of events:
  • Raw material the raw materials that will compose the product must be received and handled by trained people
  • the present invention can be applied both by spraying, bar spraying, aerial spraying, low volume, ultra low volume, planting furrow spraying, drench, coating (industrial or not), treatment (industrial or not) and incrustation (industrial or not) of seeds of plants grown for biological control of phytopathogens, phytonematodes, insects and mites, inoculants, soil conditioners, biostimulants and water stress reducers for cultivated plants and bioremediation.
  • the application method should be analyzed on a case-by-case basis and will depend on the technical conditions and needs of each producer.
  • Coffea spp Coffea arabica, Coffea canefora, Coffea robusta
  • Glycine max Zea mays, Gossypium hirsutum, vegetables (Solanum lycopersicum, Allium strain, Lactuca sativa, Daucus carota, Capsicum annuum Group), forest (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamental, fruit (Citrus spp., Malus spp., Carica papaya, Vitis spp ., Cucumis melo, Prunus Persica, Passiflora edulis), Brachiaria spp.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Trata-se a presente patente de invenção de composições biológicas de funções múltiplas, pertencente ao setor técnico de biotecnologia, para o controle biológico de fitopatógenos, fitonematóides, pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não, mais particularmente trata-se de composições contendo misturas de aditivos e excipientes a pelo menos pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes; de pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens e seus mutantes; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colônia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas, com efeitos nematicidas na redução dos danos e controle de fitonematóides, com efeitos fungicidas na redução dos danos e controle de fitopatógenos, com ação inseticida na redução dos danos e controle de insetos e ácaros, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, essa composição amplia a eficiência na condução das plantas cultivadas por utilizar mecanismos de ação diferentes e complementares das composições dos microrganismos e ativos destacados químicos ou biológicos.

Description

COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES MÚLTIPLAS
[001] Trata-se a presente patente de invenção de composições biológicas de funções múltiplas para o controle biológico de fitopatógenos, fitonematóides, pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não, mais particularmente trata-se de composições contendo misturas de aditivos e excipientes a pelo menos pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes; de pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens e seus mutantes; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas, com efeitos nematicidas na redução dos danos e controle de fitonematóides, com efeitos fungicidas na redução dos danos e controle de fitopatógenos, com ação inseticida na redução dos danos e controle de insetos e ácaros, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, para pulverização, pulverização via barra, pulverização aérea, baixo volume, ultra baixo volume, pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas. Pertencente ao setor técnico de biotecnologia, essa composição amplia a eficiência na condução das plantas cultivadas por utilizar mecanismos de ação diferentes e complementares das composições dos microrganismos e ativos destacados acima.
ESTADO DA TÉCNICA
[002] Ganhos produtivos da agricultura tem sido, muitas vezes, vinculados a aumentos gradativos de produtividade sem que ocorra um aumento na área agricultável. Esses aumentos de produtividade vêm sendo obtidos por meio de avanços significativos nas técnicas de cultivo, utilização de variedades mais adaptadas a fatores bióticos e abióticos, adequação do requerimento nutricional das plantas e, também, pelo melhor controle e redução dos danos causados por agentes fitopatogênicos e pragas agrícolas. Dentre esses avanços, o controle de pragas e doenças ainda é visto como o maior desafio na manutenção da produtividade das culturas. Diversas técnicas podem ser empregadas, com maior ou menor grau de eficácia, mas, de maneira geral, o uso de defensivos químicos continua sendo o método mais utilizado.
[003] Entretanto, para o controle de fitopatógenos, fitonematóides e pragas agrícolas, o uso de defensivos químicos tem mostrado, muitas vezes, resultados insatisfatórios. A maior parte desse problema decorre do uso indiscriminado e excessivo de moléculas químicas, que, frequentemente tem ocasionado problemas de intoxicação humana e/ou animal, concomitantemente a contaminação do meio ambiente. Esses eventos têm levado a um aumento da percepção pública contrário ao uso de defensivos químicos e à busca incessante por técnicas de manejo mais seguras ou alternativas.
[004] Assim, controles alternativos para fitonematóides, fitopatógenos e pragas agrícolas vêm sendo empregados sempre que possível, mas, adversidades inerentes à biologia desses organismos, costumam inviabilizar o uso de algumas técnicas. Por exemplo, variedades de cultivares resistentes são raramente disponíveis, enquanto que a rotação de cultura costuma ser inviável, devido aos custos económicos ou a grande gama de hospedeiros para algumas espécies (Bird et al., 2003)
[005] Nesse contexto, o controle biológico utilizando microrganismos vem sendo considerado uma opção viável, tanto para tratamento de sementes, tratamento de sulco de plantio ou pulverização das culturas de interesse.
[006] Dentre os mecanismos de ação, o antagonismo e antibiose costumam ser o modo de ação predominante de bactérias contra os fitonematóides e fungos. No caso dos fungos, competição e parasitismo são os mais presentes. Além do efeito direto na mortalidade dos fitonematóides, esses compostos nematicidas podem apresentar ação direta na eclosão de ovos ou na mobilidade e, efeitos indiretos, como alteração dos exudados radiculares, confusão na locomoção ou indução de resistência também são observados (Sikora & Hoffmann-Hergarten, 1992; Hasky-Gíinther et al. 1998). O uso de bactérias como agentes de controle biológico tem se mostrado uma alternativa promissora no sistema produtivo (Hallmann et al. 2004), como, por exemplo para Meloidogyne graminicola, onde Bacillus megaterium reduziu em 40% a penetração e formação de galhas desse fitonematóide nas raízes de arroz, além de diminuir em 60% sua migração para rizosfera e reduzir em 60% a eclosão de ovos (Sikora & Padgham 2007).
[007] Segundo Da Silva et al. (2007) o uso de Bacillus subtilis para o controle de Pratylenchus brachyurus foi tão bom quanto o tratamento químico utilizando abamectina, apresentando populações extremamente baixas destes fitonematóides no solo, cerca de 1 fitonematoide/cm3 de solo, reduzindo em tomo de 90% esta população frente ao tratamento testemunha. Outros resultados nesta linha de pesquisa, utilizando também Bacillus subtilis para tratamento de sementes é evidenciado por Da Silva et al. (2007), mostrando que esta alternativa de manejo proporcionou reduções na ordem de 50,06% da população de Pratylenchus spp. 30 dias após a semeadura, agregando 18% em produtividade. Resultados semelhantes foram encontrados por Higaki (2012) utilizando Bacillus subtilis para controle de Rotylenchulus reniformis e Pratylenchus brachyurus em algodoeiro. O tratamento com o microrganismo apresentou reduções superiores a 50% na população desses fitonematóides nas raízes da cultura. Bernardo (2002), utilizando os fungos Monacrosporium robustum, Arthrobotrys oligospora e A. musiformis obteve reduções significativas das populações de R. reniformis tanto em vitro quanto em campo.
[008] Outro dado interessante relatado por Higaki (2012), foi que as plantas tratadas com tal microrganismos apresentaram incrementos de massa fresca de raízes e na parte aérea na ordem de 36 e 47%, respectivamente, frente ao tratamento testemunha. De acordo com Araújo et al. (2008), os mecanismos de ação responsáveis pela promoção de crescimento em plantas podem estar ligados inicialmente à inibição direta do patógeno e pela indução de resistência sistémica, dentre outras. Muitas vezes é difícil reconhecer os mecanismos e relacioná-los à promoção direta de crescimento, visto que mais de um mecanismo é produzido pela bactéria.
[009] Para o controle biológico de fitopatógenos em plantas agricultáveis, Cook & Baker (1983) relatam que a caracterização de efetividade desta prática de manejo é“a redução da soma de inoculo ou das atividades determinantes da doença, provocada por um patógeno, realizada por ou através de um ou mais organismos que não o homem”. Tendo em vista esse conceito de ação, Bettiol et al. (2008) relatam que o uso de Bacillus subtilis tem obtido sucesso para controlar o oídio (causado pelo fungo Uncinula necato ) e a podridão-cinzenta (causado pelo fungo Botrytis cinerea ) da uva e em outras culturas há vários anos no Chile. Bernardo (2007) observou alta eficiência de controle em Guignardia citricarpa e Penicillium digitatum em condição de campo e pós-colheita. Segundo Mattos (2010), o uso da mistura de B. subitilis e B. licheniformis, proporcionou reduções significativas da incidência do bolor verde, causado pelo fungo Penicillium digitatum, em frutos de pós colheita da laranja“pêra”
[010] O genêro Bacillus spp. é reconhecidamente um excelente produtor de compostos antimicrobianos (Huang & Chang, 1975; Baker et al., 1983; Motomura & Hirooka, 1996) e a constatação da produção de substâncias inibidoras aos fitopatógenos, produzidas por diversos isolados de Bacillus spp., é especialmente importante para o entendimento dos mecanismos de ação desses microrganismos para o antagonismo. As bactérias antagónicas, a exemplo de B. subtilis, de modo geral agem significativamente por antibiose e, ocasionalmente, por parasitismo e competição. Dessa forma, o controle da doença pode ser efetivo devido às substâncias produzidas e, não apenas pela introdução massal dos agentes de biocontrole (Arras & Arru, 1997). Microrganismos que agem por antibiose, geralmente têm amplo espectro de ação, de forma que na inibição dos fungos a produção de substâncias tóxicas é mais efetiva do que qualquer outro mecanismo de ação envolvido.
[011] Com relação ao estresse abiótico, uma alternativa viável e com resultados significativos é o uso de rizobactérias promotoras de crescimento de plantas (RPCP). Diversos mecanismos associados às RPCPs, que auxiliam as plantas a suportarem os efeitos do estresse hídrico foram descritos, sendo eles: solubilização e fixação de nutrientes, produção de sideróforos, produção de hormônios vegetais, produção de compostos voláteis, alteração na morfologia e arquitetura das raízes, atividade da ACC deaminase, acumulação de osmólitos, ação sobre o sistema antioxidante da planta e produção de exopolissacarídeos (EPS) (ALAMI et al., 2000; ARORA et al., 2001; HUSSAIN et al., 2009; VURUKONDA et al., 2016).
[012] Dentre os mecanismos descritos, a produção de EPS por bactérias tem sido amplamente estudada, porém, visando a aplicação industrial, em especial, nas indústrias alimentícias e farmacêuticas, devido suas características emulsificantes e espessantes (KUMAR et al., 2007). Contudo, dada essas características, a utilização de bactérias produtoras de EPS na agricultura tem despertado interesse, uma vez que podem atuar como importante ferramenta na mitigação dos estresses abióticos.
[013] Apesar dessas vantagens, a maioria dos produtos existentes baseiam-se na premissa de explorar um único microrganismo para o controle biológico de fitopatógenos e fitonematóides, inoculantes, condicionadores de solo, bioestimulantes, redutores de estresse hídrico nas plantas cultivadas e biorremediação. Conforme relatado, alguns agentes possuem mais de um mecanismo de ação, os quais podem atuar isoladamente ou simultaneamente, direta ou indiretamente nos fitonematoides e fitopatógenos, no estímulo ao crescimento de plantas, no condicionamento dos solos, na redução do estresse hídrico em plantas cultivadas e biorremediação. Entretanto, associações entre os diversos agentes ainda é pouco explorado e ampliam o espectro de ação desses microrganismos, favorecendo o crescimento e proteção vegetal e consequentemente o aumento de produtividade com proteção ambiental.
[014] Como parte do estado da técnica, destacamos ainda os seguintes documentos: patente WO2018/045063, patente US 7,429,477 B2, patente US 6,808,917 Bl, patente US 2011/0110906 Al, patente US 6,194,193 Bl , BR1020180105434. OBJETIVOS DA INVENÇÃO
[015] Assim, o objetivo da presente invenção é proporcionar composições efetivas para a redução de danos causados por fitopatógenos, fitonematóides, pragas agrícolas, promover o crescimento vegetal, condicionar solos e mitigar danos causados por eventuais estresses hídricos ou contaminação ambiental.
DESCRIÇÃO RESUMIDA DA INVENÇÃO
[016] A presente invenção refere-se a composições biológicas de funções múltiplas, para pulverização, pulverização via barra, pulverização aérea, baixo volume, ultra baixo volume, pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, contendo misturas de pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes; de pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales e seus mutantes ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas em composições biológicas com propriedades fungicidas, nematicidas, inseticidas estimulantes de crescimento, condicionadoras de solos e redução dos efeitos de estresse hídrico em plantas cultivadas e biorremediação e áreas cultivadas. DESCRIÇÃO DETALHADA DA INVENÇÃO
[017] As composições biológicas de funções múltiplas, compreendem: 1,0 a 90,0% de pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes;
1,0 a 90,0 % de pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales e seus mutantes ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.);
5,0 a 10,0% de defensivos agrícolas;
5,0 a 20,0% de óleo essencial;
5,0 a 20,0% de extrato de plantas;
1,0 a 15,0% de Aditivos; e
1,0 a 97,0% de Excipientes.
[018] Os Bacillus que compreendem a ordem Bacilales e seus mutantes são do grupo que consistem em Bacillus velezensis, Bacillus aryabhattai, Bacillus genus, Bacillus pseudomycoides, Bacillus mucilaginosus, Bacillus sonorensis, Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus licheniformis, Bacilus paralicheniformis, Bacillus amyloliquefasciens, Bacillus megaterium, Bacillus sphaericus, Bacillus thuringiensis e qualquer outro gênero ou espécie da ordem supra-citada.
[019] Os fungos e seus mutantes são do grupo que compreende a Hypocreales, não restringindo aos gêneros e espécies de Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamomeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, Trichoderma virens, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora, Clonostachys farinosa, Clonostachys pseudobotrytis, Clonostachys pulvinata, Clonostachys rosea, (Link) Schroers, Clonostachys spectabilis, Clonostachys theobromae, Purpuriocillium lilacinus, Pochonia bulbillosa, Pochonia chlamydosporia, Pochonia chlamydosporia var. catenulata, Pochonia globispora, Pochonia goniodes, Pochonia humicola, Pochonia microbactrospora, Pochonia parasitica, Pochonia rubescens, Pochonia suchlasporia, Metarhizium anisopliae, Metarhizium acridum, Metarhizium brunneum, Metarhizium flavoviride, Metarizium rileyi, Aschersonia cubensi, Aschersonia aleyrodis, Aschersonia australiensis Aschersonia placenta, Isaria fumosorosea, Lecanicillium lecanni, Lecanicillium longisporum, Lecanicillium muscarium, Lecanicillium fungicola, Cordyceps militaris, Harposporium anguillulae e qualquer outro gênero ou espécie da ordem supra-citada.
[020] Os fungos e seus mutantes são do grupo que compreende a ordem Orbiliales, não restringindo aos gêneros e espécies de Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conoides, Arthrobotrys constringens, Arthrobotrys cylindrospora, Arthrobotrys dactyloides, Arthrobotrys elegans Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Arthrobotrys irregulares, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora, Dactylaria haptotyla, Dactylaria. purpurella, Dactylaria scaphoides, Dactylaria brochopaga, Dactylaria candida e qualquer outro gênero ou espécie da ordem supra-citada.
[021] Os fungos e seus mutantes são do grupo que compreende a ordem Helotiales, não restringindo aos gêneros e espécies de Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum, Monacrosporium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum, Monacrosporium cionopagu, Dactylella alba, Dactylella aphrobrocha, Dactylella arcuata, Dactylella arnaudii, Dactylella atractoides, Dactylella attenuata, Dactylella candida, Dactylella cionopaga, Dactylella fusiformis, Dactylella heterospora, Dactylella intermédia, Dactylella oxyspora, Dactylella ramosa e qualquer outro gênero ou espécie da ordem supra-citada.
[022] Os fungos e seus mutantes são do grupo que compreende a ordem Mucorales, não restringindo aos gêneros e espécies de Cunninghamella elegans e qualquer outro gênero ou espécie da ordem supra-citada.
[023] Os defensivos agrícolas consistem em:
a) Inseticidas - capsaicin, cartap, celastrus-extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, chlorpyrifos-e, cnidiadin, cryolite, cyanophos, cyantraniliprole, cyclaniliprole, cyhalothrin, cyhexatin, cypermethrin, dacnusa, DCIP, dichloropropene, dicofol, diglyphus, diglyphus+dacnusa, dimethacarb, dithioether, dodecylacetate, emamectin, encarsia, EPN, eretmocerus, ethylene-dibromide, eucalyptol, fatty-acids, fatty-acids/salts, fenazaquin, fenobucarb (BPMC), flubrocythrinate, flufenzine, formetanate, formothion, furathiocarb, gamma-cyhalothrin, vírus da granulose, NPV, acido indol-3-ylbutyric, iodomethane, isocarbofos, isofenphos, isofenphos-m, isoprocarb, isothioate, kaolin, lindane, liuyangmycin, mephosfolan, metaldehyde, methamidophos, metolcarb (MTMC), óleo mineral, m-isothiocyanate, monosultap, oleic-acid, omethoate, orius, oxymatrine, paecilomyces, parathion-e, pasteuria, pheromones, phosphorus-acid, photorhabdus, phoxim, phytoseiulus, pirimiphos-e, vírus da poliedrose, , oleato de potassium, prothiofos, pyraclofos, pyrethrinas, pyridaphenthion, pyrimidifen, pyriproxifen, sulfluramid, tebupirimfos, teflutbrin, temephos, tetradifon, thiofanox, thiometon, triazamate, triflumuron, vertrine, kappa-bifenthrin, kappa-tefluthrin, dichoromezotiaz, broflanilide, pyraziflumid aldicarb, alanycarb, benfuracarb, carbaryl, carbofuran, carbosulfan, methiocarb, methomyl, oxamyl, pirimicarb, propoxur e thiodicarb, acephate, azinphos-ethyl, azinphos-methyl, chlorfenvinphos, chlorpyrifos, chlorpyrifos-methyl, demeton-Smethyl, diazinon, dichlorvos/DDVP, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidaphos, methidathion, mevinphos, monocrotophos, oxymethoate, oxydemeton-methyl, parathion, parathion- methyl, phenthoate, phorate, phosalone, phosmet, phosphamidon, pirimiphos- methyl, quinalphos, terbufos, tetrachlorvinphos, triazophos, trichlorfon, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, spinosad, spinetoram, mectins, abamectin, emamectin benzoate, ivermectin, lepimectin, milbemectin, hydroprene, kinoprene, methoprene, fenoxycarb, pyriproxyfen; pymetrozine, flonicamid, pyrifluquinazon, clofentezine, hexythiazox, etoxazole, diafenthiuron, fenbutatin oxide, propargite, chlorfenapyr, bensultap, cartap hydrochloride, thiocydam, thiosultap sodium, bistrifluron, diflubenzuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, teflubenzuron, buprofezin, cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, amitraz, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim, cyenopyrafen, cyflumetofen, hydramethylnon, acequinocyl, fluacrypyrim, indoxacarb, metaflumizone, spirodiclofen, spiromesifen, spirotetramat, flubendiamide, chlorantraniliprole, cyclaniliprole, cyantraniliprole, azadirachtin, amidoflumet, bifenazate, fluensulfone, piperonyl butoxide, pyridalyl, sulfoxaflor, acrinathrin, allethrin, bifenthrin, cyfluthrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, tau-fluvalinate, permethrin, silafluofen e tralomethrin, dentre outros;
b) Fungicidas: benzovindiflupyr, antiperonosporic, ametoctradin, amisulbrom, hidróxido de cobre, oxychloride de cobre, cobre, sulfate, persulfate de cobre, boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, isofetamid, fenfuram, bixafen, fluxapyroxad, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyrametostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, fentin hydroxide, oxytetracycline, chlozolinate, chloroneb, tecnazene, etridiazole, iodocarb, prothiocarb, pyrisoxazole, oxpoconazole, etaconazole, fenpyrazamine, fenpicoxamide, mefentrifluconazole, naftifine, terbinafine, validamycin, pyrimorph, valifenalate, fthalide, probenazole, isotianil, laminarin, teclofthalam, triazoxide, pyriofenone, chlorothalonil, fluoroimide, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxiconazole, fluquinconazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, simeconazole, triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamid, benomyl, carbendazim, thiabendazole, fuberidazole, ethaboxam, etridiazole and hymexazole, azaconazole, diniconazole-M, oxpoconazol, paclobutrazol, uniconazol, l-(4- chloro-phenyl)-2-([l,2,4]triazol-lyl)-cycloheptanol, imazalilsulfphate, azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, methominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, enestroburin, methyl (2-chloro-5-[l-(3- methylbenzyloxyimino)ethyl]benzyl)carbamate, methyl (2-chloro-5-[l-(6- methylpyridin-2- ylmethoxyimino)ethyl]benzyl)carbamate, methyl 2-(ortho- (2,5-dimethylphenyloxymethylene)- phenyl)-3-methoxyacrylate, 2-(2-(6-(3- chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)- phenyl)-2- methoxyimino-N-methyl-acetamide, 3-methoxy-2-(2-(N-(4-methoxy-phenyl)- cyclopropanecarboximidoylsulfanylmethyl)-phenyl)-acrylic acid methyl ester; carboxin, benalaxyl, benalaxyl-M, fenhexamid, flutolanil, furametpyr, mepronil, metalaxyl, mefenoxam, ofurace, oxadixyl, oxycarboxin, penthiopyrad, isopyrazam, thifluzamide, tiadinil, 3,4-dichloro-N-(2- cyanophenyl)isothiazole-5-carboxamide, dimethomorph, flumorph, flumetover, fluopicolide (picobenzamid), zoxamide, carpropamid, diclocymet, mandipropamid, N- (2-(4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3- methoxyphenyl)ethyl)-2-methanesulfonyl-amino-3- methylbutyramide, N-(2- (4-[3-(4-chloro-phenyl)prop-2-ynyloxy]-3-methoxy-phenyl)ethyl)-2- ethanesulfonylamino- 3 -methylbutyramide, methyl 3-(4-chlorophenyl)-3-(2- isopropoxycarbonylamino-3-methyl-butyrylamino)propionate, N-(4'- bromobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N- (4'-trifluoromethyl-biphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5- carboxamide, N-(4'- chloro-3'-fluorobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-3- difluoro-methyl-l-methylpyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-3-difluoromethyl-lmethylpyrazole-4-carboxamide, N-(2- cyano-phenyl)- 3,4-dichloroisothiazole-5-carboxamide, 2- amino-4-methyl- thiazole-5-carboxanilide, 2-chloro-N-(l,l,3-trimethyl-indan-4-yl)- nicotinamide, N- (2-(l,3-dimethylbutyl)-phenyl)-l,3-dimethyl-5-fluoro-lH- pyrazole-4-carboxamide, N-(4'-chloro3',5-difluoro-biphenyl-2-yl)-3- difluoromethyl-l-methyl-IH-pyrazole-4-carboxamide, N-(4'-chloro3',5- difluoro-biphenyl- 2-yl)-3-trifluoromethyl-l -methyl-lH-pyrazole-4- carboxamide, N-(3',4'- dichloro-5-fluoro-biphenyl-2-yl)-3-trifluoromethyl-l- methyl-lH-pyrazole-4- carboxamide, N-(3',5difluoro-4'-methyl-biphenyl-2- yl)-3-difluoromethyl- 1 -methyl-lH-pyrazole-4-carboxamide, N- (3',5- difluoro-4'-methyl-biphenyl-2-yl)-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N- (cis-2-bicyclopropyl-2-yl-phenyl)-3-difluoromethyl-l methyl-lH-pyrazole-4-carboxamide, N- (trans-2-bicyclopropyl-2-yl-phenyl)- 3-difluoro-methyl-l-methyl-l H-pyrazole-4-carboxamide, fluopyram, N-(3- ethyl-3,5-5- trimethyl-cyclohexyl)-3-formylamino-2-hydroxy-benzamide, oxytetracyclin, silthiofam, N-(6-methoxy-pyridin-3-yl) cyclopropanecarboxamide, 2-iodo-N-phenylbenzamide, N-(2-bicyclo-propyl- 2-yl-phenyl)-3-difluormethyl-l-methylpyrazol-4-ylcarboxamide, N- (3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethyl-5-fluoropyrazol-4-yl-carboxamide, N- (3',4',5'-trifluorobiphenyl-2-yl)-5-chlorol,3-dimethyl- pyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl- lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-lmethylpyrazol-4-ylcarboxamide,N-(3',4',5'- trifluorobiphenyl-2-yl)-3 -difluoromethyl-lmethylpyrazol-4-ylcarboxamide, N - (3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-lmethylpyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-l - methylpyrazol-4-ylcarboxamide, N- (3', 4', 5'-trifluorobiphenyl-2-yl)-3- (chlorodifluoromethyl)-lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5- chloro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)- 1 ,3-dimethyl-5-fluoropyrazol-4-ylcarboxamide, N- (2, ,4 , ,5'- trifluorobiphenyl-2-yl)-5-chloro-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-fluoromethyl-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-l-methylpyrazol-4-ylcarboxamide,N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2- yl)-5-chloro- 3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N- (2',4',5'- trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-l-methylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5 trifluorobiphenyl-2-yl)- 5-fluoro-l-methyl-3-trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-5-chloro-l-methyl-3-trifluoromethylpyrazol-4- ylcarboxamide, N-(3',4'- dichloro-3-fluorobiphenyl-2-yl)-l -methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'- dichloro-3- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-l H-pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl- S-difluoromethyl-1 H-pyrazole-4-carboxamide, N-(3'- chloro-4'-fluoro-3- fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3M'-dichloro-4-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-difluoro-4-fluorobiphenyl-2-yl)-l - methyl- S-trifluoromethyl-lH-pyrazole-4-carboxamide, N- (3 ',4'-dichloro-4- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4- carboxamide, N- (3M'-difluoro-4-fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH- pyrazole-4-carboxamide, N- (3'-chloro-4'-fluoro-4-fluorobiphenyl-2-yl)-l- methyl-5-difluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-l - methyl- S-difluoromethyl-IH-pyrazole-carboxamide, N- (3',4'-difluoro-5- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3',4'-dichloro-5-fluorobiphenyl-2-yl)-l,3-dimethyl-l H-pyrazole-4- carboxamide, N-(3'-chloro-4'- fluoro-5-fluorobiphenyl-2-yl)-l-methyl-3- difluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-4-fluorobiphenyl-2- yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'-fluoro- 5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide,N-(4'- chloro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl- lH-pyrazole-4-carboxamide, N-(4'- methyl-5-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-5-fluorobiphenyl- 2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-chloro-5- fluorobiphenyl-2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-methyl- 5-fluorobiphenyl-2- yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4- carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-[2-(l, 1,2, 3,3,3- hexafluoropropoxy)-phenyl]-3- difluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]- 3-difluoromethyl-l- methyl-lH-pyrazole-4-carboxamide and N-[4'-(trifluoromethylthio)- biphenyl2-yl]-l-methyl-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, fluazinam, pyrifenox, bupirimate, cyprodinil, fenarimol, ferimzone, mepanipyrim, nuarimol, pyrimethanil, triforine, fenpiclonil, fludioxonil, aldimorph, dodemorph, fenpropimorph, tridemorph, fenpropidin, iprodione, procymidone, vinclozolin, famoxadone, fenamidone, octhilinone, probenazole, 5-chloro-7-(4-methyl-piperidin-l-yl)-6-(2,4,6- trifluorophenyl)-
[1.2.4]triazolo[l,5-a]pyrimidine, anilazine, diclomezine, pyroquilon, proquinazid, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, acibenzolar-S-methyl, captafol, captan, dazomet, folpet, fenoxanil, quinoxyfen, N,N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-lsulfonyl)-
[1.2.4]triazole-l-sulfonamide, 5-ethyl-6-octyl-[l,2,4]triazolo[l,5- a]pyrimidin- 2,7-diamine, 2,3,5,6-tetrachloro-4-methanesulfonyl-pyridine, 3,4,5-trichloro- pyridine-2,6-di-carbonitrile, N-(l- (5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2,4- dichloro-nicotinamide, N-((5-bromo-3-chloropyridin-2- yl)-methyl)-2,4- dichloro-nicotinamide, diflumetorim, nitrapyrin, dodemorphacetate, fluoroimid, blasticidin-S, chinomethionat, debacarb, difenzoquat, difenzoquat-methylsulphat, ácido oxolinic, piperalin, mancozeb, maneb, metam, methasulphocarb, metiram, ferbam, propineb, thiram, zineb, ziram, diethofencarb, iprovalicarb, benthiavalicarb, propamocarb, propamocarb hydrochlorid, 4-fluorophenyl N-(l-(l-(4-cyanophenyl)-ethanesulfonyl)but-2- yl)carbamate, methyl 3-(4-chloro-phenyl)-3-(2- isopropoxycarbonylamino-3- methylbutyrylamino) propanoate, guanidine, iminoctadine, guazatine, kasugamycin, oxytetracyclin, streptomycin, polyoxin, validamycin, binapacryl, dinocap, dinobuton, sulfur-containing heterocyclyl compounds: dithianon, isoprothiolane, edifenphos, iprobenfos, fosetyl, fosetyl-aluminum, pyrazophos, tolclofos- methyl, dichlofluanid, flusulfamide, hexachloro- benzene, phthalide, pencycuron, quintozene, thiophanate, thiophanatemethyl, cyflufenamid, cymoxanil, dimethirimol, ethirimol, furalaxyl, metrafenone and spiroxamine, guazatine-acetate, iminoc- tadine-triacetate, iminoctadinetris(albesilate), kasugamycin hydrochloride hydrate, dichlorophen, pentachlorophenol and its salts, N-(4- chloro-2-nitro-phenyl)- N-ethyl-4-methyl-benzenesulfonamide, dicloran, nitrothal-isopropyl, tecnazen, biphenyl, bronopol, diphenylamine, mildiomycin, oxincopper, prohexadione calcium, N - (cyclopropylmethoxyimino- (6-difluoromethoxy- 2,3-difluoro-phenyl)-methyl)-2-ph acetamide, N'-(4-(4-chloro-3- trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl
formamidine, N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl- phenyl)-N-ethyl-N-methyl formamidine, N'-(2-methyl-5-trifluormethyl-4-(3- trimethylsilanyl-propoxy)-phenyl)-N-ethyl-Nmethylformamidine and N'-(5- difluormethyl-2-methyl- 4-(3-trimethylsilanyl-propoxy)-phenyl)-Nethyl-N- methyl formamidine;
c) Herbicidas: alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; éster de phenoxyphenoxypropionic, clodinafop-propargyl, cyhalofop-butyl, diclofop- methyl, fenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop- butyl, fluazifop-P-butyl, haloxyfop-ethoxyethyl, haloxyfop-methyl, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl ou quizalofop-tefuryl, flamprop-methyl, flamprop- isopropyl, imazapyr, imazaquin, imazamethabenz-methyl (imazame), imazamox, imazapic, imazethapyr, pyrithiobac-acid, pyrithiobac-sodium, bispyribac-sodium, pyribenzoxym, florasulam, flumetsulam, metosulam, amidosulfuron, azimsulfuron, bensulfuron-methyl, chlorimuron-ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron-methyl, ethoxysulfuron, flazasulfuron, halosulfuron-methyl, imazosulfuron, metsulfuron-methyl, nicosulfuron, primisulfuron-methyl, prosulfuron, pyrazosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, thifensulfuron- methyl, triasulfuron, tribenuron-methyl, triflusulfuron-methyl, tritosulfuron, sulfosulfuron, foramsulfuron, iodosulfuron, benzoyl prop-ethyl, bromobutide, chiorthiamid, diphenamid, etobenzanidibenzchlomet, fluthiamide, fosamin, monalide, clopyralid, picloram, 2,4-D, benazolin, naptalame, diflufenzopyr, benzofenap, clomazone, diflufenican, fluorochloridone, fluridone, pyrazolynate, pyrazoxyfen, isoxaflutole, isoxachlortole, mesotrione, sulcotrione, ketospiradox, flurtamone, norflurazon, amitrol, glyphosate, sulfosate, bilanafos, glufosinateammonium, anilofos, mefenacet, dimethenamid, S-dimethenamid, acetochlor, alachlor, butachlor, butenachlor, diethatyl-ethyl, dimethachlor, metazachlor, metolachlor, S-metolachlor, pretilachlor, propachlor, prynachlor, terbuchlor, thenylchlor, xylachlor, butylate, cycloate, di-allate, dimepiperate, EPTC. esprocarb, molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, tiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butamifos, chlorthal-dimethyl (DCPA), acifluorfen, acifluorfen-sodium, aclonifen, bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl, oxadiazon, azafenidin, butafenacil, carfentrazone- ethyl, cinidon-ethyl, flumiclorac-pentyl, flumioxazin, flumipropyn, flupropacil, fluthiacet-methyl, sulfentrazone, thidiazimin, nipyraclofen, propanil, pyridate or pyridafol, bromofenoxim, dinoseb, dinoseb-acetate, dinoterb, cyperquat-chloride, difenzoquat-methylsulfate, diquat or paraquatdichloride, chlorbromuron, chlorotoluron, difenoxuron, dimefuron, diuron, ethidimuron, fenuron, fluometuron, isoproturon, isouron, linuron, methabenzthiazuron, methazole, metobenzuron, metoxuron, monolinuron, neburon, siduron, tebuthiuron, bromoxynil, ioxynil, chloridazon, ametryn, atrazine, cyanazine, desmein, dimethamethryn, hexazinone, prometon, prometryn, propazine, simazine, simetryn, terbumeton, terbutryn, terbutylazine, trietazine, metamitron, metribuzin bromacil, lenacil, terbacil, desmedipham, phenmedipham, tridiphane, isoxaben, dichlobenil, dalapon, ethofumesate, chlorfenac, aziprotryn, barban, bensulide, benzthiazuron, benzofluor, buminafos, buthidazole, buturon, cafenstrole, chlorbufam, chlorfenprop-methyl, chloroxuron, cinmethylin, cumyluron, cycluron, cyprazine, cyprazole, dibenzyluron, dipropetryn, dymron, eglinazin-ethyl, endothall, ethiozin, flucabazone, fluorbentranil, flupoxam, isocarbamid, isopropalin, karbutilate, mefluidide, monuron, napropamide, napropanilide, nitralin, oxaciclomefone, phenisopham, piperophos, procyazine, profluralin, pyributicarb, secbumeton, sulfallate (CDEC), terbucarb, triaziflam, triazofenamid, trimeturon, dentre outros;
d) Nematicidas: Benomyl, cloethocarb, aldoxycarb, tirpate, diamidafos, fenamiphos, cadusafos, dichlofenthion, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofof, isazofos, phosphocarb, thionazin, imicyafos, mecarphon, acetoprole, benclothiaz, chloropicrin, dazomet, fluensulfone, 1,3-dichloropropene (telone), dimethyl disulfide, metam sodium, metam potassium, methyl bromide, allyl isothiocyanate (AITC), dimethyl sulfate, furfual (aldehyde), dentre outros.
[024] Os óleos essenciais e extratos de plantas obtidos de sementes, plantas e parte de plantas são do grupo que consiste em: Ricinus communis, Crotalaria juncea, Chenopodium ambrosioides, Azadirachta indica, Verbena officinalis, Erythrina mulungu, Quassia amara, Bidens pilosa, Plantago lanceolata, Senecio brasiliensis, Lantana câmara, Tagetes patula, Lantana montevidensis, Eucalyptus spp., Lantana montevidensis, Lantana câmara, Senecio brasiliensis, Chrysantemum coronarium, Solanum americanum, Solanum paniculatum Hovenia dulcis, Melia azedarach, Butia eriospatha, Schinus terenbithifolius, Cinnamomum camphora, Conyza bonariensis, Senecio brasiliensis, Bidens pilosa, Amaranthus hybridus, Euphorbia heterophylla, Raphanus sativus, Ipomoea purpúrea, Brachiaria plantaginea, Ruía graveolens, Aloysia triphylla, Brassica napus, Mucuna pruriens, Ocimum gratissimum, Lippia sidoides, Croton zehntneri, Mentha piperita, Cymbopogon martinii, Cymbopogon schoenanthus, Piper aduncum, Arisaema franchetianum, Zanthoxylum simulans, Ruta chalepensis, Citrus sinensis, Melaleuca quinquenervia, Cymbopogon citratus, Thymus vulgaris, Piper aduncum, Melaleuca alternifolia, Hesperozygis myrtoides e demais espécies dos gêneros supra-citados.
[025] Os aditivos são dispersantes selecionados do grupo consistindo de dispersantes selecionados do grupo consistindo de polímeros iônicos solúveis em água, aminoácidos, extratos de algas, compostos de origem natural, polímeros aniônicos solúveis em água, surfactantes selecionados do grupo consistindo de surfactantes aniônicos e surfactantes não iônicos e combinações entre eles e derivados. [026] Os excipientes são do grupo que consiste em: sílicas, talco, bentonita, carboidratos, açúcares, amidos, carbonatos, caseína, soro de leite e derivados de leite e combinações entre eles e derivados.
[027] As composições podem ser empregadas como formulação de suspensão de encapsulado, concentrado dispersível, concentrado emulsionável, emulsão de água em óleo, emulsão de óleo em água, microemulsão, suspensão concentrada, suspo-emulsão, granulado solúvel, concentrado solúvel, pó solúvel, tablete, granulado dispersível, pó molhável, gel emulsionável, gel solúvel em água, grânulo emulsionável, pó emulsionável, dispersão de óleo ou suspensão concentrada em óleo, formulação mista de concentrado solúvel e susensão concetrada, formulação mista de concentrado sóluvel e suspo-emulsão, formulação mista de concentrado solúvel e emulsão de óleo em água, suspensão concentrada dispersível ou miscível em óleo, solução miscível em óleo, pó dispersível em óleo, granulado encapsulado, pó seco, líquido para pulverização eletrostática/eletrodinâmica, granulado, óleo para pulverização/espalhamento, suspensão a ultrabaixo volume, ultrabaixo volume, microgranulado, pó fino, granulado fino, macrogranulado, encapsulamento, microencapsulamento e nanoencapsulamento.
[028] As composições biológicas de funções múltiplas são para o controle biológico fitopatógenos, fitonematóides e pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não com efeitos nematicidas na redução dos danos e controle de fitonematóides, não se limitando à Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incógnita, Meloidogyne javanica, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Meloidogyne exigua, Helicotilechus dihystera, Meloidogyne exigua, Meloidogyne paranaensis, Meloidogyne coffeicola, Meloidogyne hapla, Meloidogyne enterolobii, Meloidogyne arabicidae, Meloidogyne arenaria, Pralylenchus brachiurus, Pralylenchus coffea, Pratylenchus zeae Xiphinema krugi, Heterodera glycines, Helicotylenchus sp., Paratrichodorus sp., Tylenchorhynchus sp., Scutellonema sp., Aphelenchoides spp., Aphelenchus spp., Mesocriconema sp., Tylenchus sp., dentre outros, com efeitos fungicidas na redução dos danos e controle, não se limitando à Pkakopsora pachyrhizi, Corynespora cassiicola, Puccinia polysora, Exserohilum lurei anu, Xanthomonas axonopodis, Ranudaria areola, Hemileia vastatrix, Cercospora coffeicola, Phoma spp., Pseudomonas syringae, Pseudomonas corrugata, Rhizoctonia solani, Fusarium solani, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Colletotrichum gloeosporioides, Colletotrichum truncatum, Colletotrichum lindemuthianum, Macrophomna phaseolina, Sclerotinia sclerotiorum, Botrytis cinerea, Penicillium digitatum, com ação inseticida e acaricida na redução dos danos e controle, não se limitando a pragas Polyphagotarsonemus latus, Tetranychus urticae, Diabrotica speciosa, Anticarsia gemmatalis, Spodoptera cosmioides, Spodoptera frugiperda, Pseudoplusia includens, Trichoplusia ni, Bemisia tabaci, Euschistos heros, Nezara viridula, Leucoptera coj eella, Hypothenemus hampei, Oligonychus ilicis, Polyphagotarsonemus latus , Brevipalpus phoenicis, Planococcus citri, Planococcus minor, Helicoverpa armigera, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, em adição aos polímeros/excipientes em formulações para pulverização, pulverização via barra, pulverização aérea, baixo volume, ultra baixo volume, pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, não se limitando a Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp. , Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis ), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, crotalárias ( Crotalaria juncea, Crotalária spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora ) e demais espécies dos gêneros supra-citados
[029] A título de exemplo, na obtenção de pelo menos 2 (dois) gêneros e/ou espécies de: Bacillus e seus mutantes; Bacillus em misturas com fungos das Ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas, temos:
Exemplo 1:
• Bacillus amyloliquefasciens (cepa CPQBA 040-11 DRM 01) - 1,0 x 109 u.f.c./g
• Bacillus amyloliquefasciens ( cepa CPQBA 040-11 DRM 04) 1,0 x 109 u.f.c./g Exemplo 2:
• Bacillus amyloliquefasciens: 1,0 x 109 u.f.c./g
• Bacillus liqueniformis: 1,0 x 109 u.f.c./g
Exemplo 3:
• Bacillus subtilis : 1,0 x 109 u.f.c./g
• Bacillus amyloliquef adens: 1,0 x 109 u.f.c./g
• Trichoderma harzianum : 1,0 x 107u.f.c./g
Exemplo 4:
• Beauveria bassiana : 1,0 x 109 u.f.c./g
• Bacillus thuringiensis: 1,0 x 109 u.f.c./g
• Aschersonia aleyrodis: 1,0 x 107u.f.c./g
Exemplo 5:
• Beauveria bassiana: 1,0 x 106 u.f.c./g
• Isaria fumosorosea : 1,0 x 106 u.f.c./g
• Aschersonia aleyrodis: 1,0 x 106u.f.c./g
Exemplo 6:
• Beauveria bassiana: 1,0 x 106 u.f.c./g
• Aschersonia aleyrodis: 1,0 x 106u.f.c./g
[030] A título de exemplo a composição poderá apresentar as seguintes concentrações:
Exemplo 1 :
Bacillus amyloliquefasciens ( cepa CPQBA 040-11 DRM 01 ): 1,0 a 20,0% Bacillus amyloliquefasciens ( cepa CPQBA 040-11 DRM 04): 1,0 a 20,0% Aditivos: 1,0 a 20,0%
Excipientes: 97,0 a 40,0%
Exemplo 2:
Bacillus amyloliquefasciens: 1,0 a 20,0% Bacillus liqueniformis: 1,0 a 20,0%
Oleo de Melaleuca quinquenervia 10,0 a 15,0% Aditivos: 1,0 a 15,0%
Excipientes: 86,0 a 30,0%
Exemplo 3:
Bacillus subtilis : 1,0 a 20,0%
Bacillus amyloliquefaciens : 1,0 a 20,0% Trichoderma harzianum : 1,0 a 20,0% Azoxistrobin 5,0 a 10,0% Aditivos: 1,0 a 20,0%
Excipientes: 92,0 a 10,0%
Exemplo 4:
Beauveria bassiana : 10,0 a 20,0%
Bacillus thuringiensis : 10,0 a 20,0% Aschersonia aleyrodis : 10,0 a 20,0%
Aditivos: 1,0 a 20,0%
Excipientes: 69,0 a 20,0%
Exemplo 5:
Beauveria bassiana : 10,0 a 20,0% Isariafumosorosea : 10,0 a 20,0%
Aschersonia aleyrodis : 10,0 a 20,0%
Aditivos: 1,0 a 20,0%
Excipientes: 69,0 a 20,0%
Exemplo 6:
Beauveria bassiana : 10,0 a 20,0%
Aschersonia aleyrodis: 10,0 a 20,0%
Aditivos: 1,0 a 20,0% Excipientes: 69,0 a 40,0%
EXEMPLOS DE OBTENÇÃO DA COMPOSIÇÃO
[031] Uma composição contendo 10,0% de Bacillus amyloliquefasciens (cepa CPQBA 040-11 DRM 01), 10,0% de Bacillus amyloliquefasciens (cepa CPQBA 040-11 DRM 04), 3% de polímero de estireno acrílico, 2,0% surfactante aniônico e 75,0% de inerte foi formulada para avaliar sua eficiência no controle de fitopatógenos, biostimulação, promoção de crescimento e condicionamento de solos.
[032] Uma composição contendo 10,0% de Bacillus subtilis, 10,0% de Bacillus amyloliquefasciens, 10,0% de óleo de Malaleuca quinquenervia, 6,0 % surfactante aniônico e 64,0% de inerte foi formulada para avaliar sua eficiência no controle de fitopatógenos.
[033] Uma composição contendo 10,0% de Bacillus amyloliquefasciens, 8,0% de Bacillus licheniformis , 10% de Trichoderma harzianum 18,0% de Azoxistrobin, 7,5% surfactante aniônico, e 46,5% de inerte foi formulada para avaliar sua eficiência no controle de fitopatógenos, controle de nematoides, promoção de crescimento e condicionamento de solos.
[034] Uma composição contendo 15,0% de Beauveria bassiana, 7,0% de Bacillus thuringiensis , 20,0% de Aschersonia aleyrodis, 4,0% de polímero de estireno acrílico, 2,5% surfactante aniônico e 51,5% de inerte foi formulada para avaliar sua eficiência no controle de insetos.
[035] Uma composição contendo 15,0% de Beauveria bassiana, 15,0% de Isaria fumosorosea, 20,0% de Aschersonia aleyrodis, 4,0% de polímero de estireno acrílico, 2,5% surfactante aniônico e 43,5% de inerte foi formulada para avaliar sua eficiência no controle de insetos.
[036] Uma composição contendo 15,0% de Beauveria bassiana, 20,0% de Aschersonia aleyrodis, 4,0% de polímero de estireno acrílico, 2,5% surfactante aniônico e 58,5% de inerte foi formulada para avaliar sua eficiência no controle de insetos.
[037] As composições para o controle biológico de fitopatógenos, fitonematóides e insetos, inoculantes, condicionadores de solo, bioestimulantes, redutores de estresse hídrico em plantas e biorremediação deverá seguir o seguinte fluxo de eventos:
• Matéria prima: as matérias primas que irão compor o produto deverão ser recebidas e manipuladas por pessoas treinadas;
• Mistura: as matérias primas deverão ser pesadas e misturadas seguindo o procedimento operacional padrão (POP) nas proporções indicadas;
• Amostragem: após a mistura, deverão ser retiradas amostras para verificação e certificação das garantias do produto. Deve-se analisar número de unidades formadoras de colónias (u.f.c./g ou u.f.c/mL) de produto;
• Envase: o produto formulado que se apresentar dentro das especificações de garantia deverão ser envasados em frascos plásticos de 1,0; 5,0 e 10,0 kg/L, previamente rotulados. Os frascos/pouchs são selados e fechados;
• Armazenagem: os frascos/pouchs são acomodados em pallets e armazenados em local seco, arejado e protegido da luz, permanecendo nessa condição até ser despachado.
[038] O presente invento poderá ser aplicado tanto por pulverização, pulverização via barra, pulverização aérea, baixo volume, ultra baixo volume, pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas para o controle biológico de fitopatógenos, fitonematóides, insetos e ácaros, inoculantes, condicionadores de solo, bioestimulantes e redutores de estresse hídrico para as plantas cultivadas e biorremediação. O método de aplicação deverá ser analisado caso a caso e dependerá das condições técnicas e necessidade de cada produtor.
VANTAGENS OBTIDAS
[039] O uso da composição para o controle biológico de fitopatógenos, fitonematóides, insetos e ácaros, inoculantes, condicionadores de solo, bioestimulantes e redutores de estresse hídrico para as plantas cultivadas e biorremediação contendo misturas de pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes; pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales e seus mutantes ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas, oferecem as seguintes vantagens:
• Favorece desenvolvimento das plantas cultivados, propiciando incrementos nos parâmetros de crescimento radicular, foliar e de produção;
• E uma alternativa ao uso de fungicidas, nematicidas, inseticidas e acaricidas químicos, vindo ao encontro do interesse da sociedade para produtos mais ecologicamente seguros;
• Explora uma gama maior de mecanismos de ação contra fitonematóides, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação contra fitopatógenos, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação contra insetos e ácaros, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação na fixação de nitrogénio nas plantas, garantindo uma maior eficiência nutricional;
• Explora uma gama maior de mecanismos no condicionamento dos solos, reduzindo os fitopatógenos e aumentando a interação com as raízes e promovendo o crescimento radicular e foliar das plantas cultivadas;
• Explora uma gama maior de mecanismos de ação contra estresses abióticos, principalmente estresse hídrico, garantindo, assim, uma maior eficiência na retenção de água e incremento produtivo;
• Explora diferentes mecanismos de ação na degradação de substâncias danosas ao meio ambiente, a exemplo de pesticidas;
• Diminui a seleção de fitonematóides, fitopatógenos e insetos resistentes a produtos químicos;
• Possui facilidade operacional para o emprego da tecnologia na pulverização, pulverização de sulco, drench e recobrimento, tratamento e incrustação de semente de forma industrial ou não;
• E capaz de ser empregado nos constituintes do revestimento, tratamento e incrustação de sementes (industrial ou não) de Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis ), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, crotalárias ( Crotalaria juncea, Crotaldria spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora ) e demais espécies dos gêneros supra-citados.
[040] A abrangência da presente patente de invenção, não deve ser limitada aos exemplos de aplicação, mas sim, aos termos definidos nas reivindicações e seus equivalentes.
REFERÊNCIAS BIBLIOGRÁFICAS
Alami, Y. Achouak W, Marol C, Heulin T. Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp. Strain isolated from sunflower roots. Applied and environmental microbiology, v. 66, n. 8, p. 3393-3398, 2000.
Araújo, F.F. Inoculação de sementes com Bacillus subtilis, formulado com farinha de ostra e desenvolvimento de milho, soja e algodão. Ciências e Agrotecnologia, Lavras, v. 2, p. 456-462. 2008
Araújo, J. M. Estratégias para isolamento seletivo de Actinomicetos. In: Melo, I. S. & Azevedo, J. L. Ecologia Microbiana. Jaguariúna: Embrapa, 1998. p.327-347.
Arora, N. K.; Kang, S. C.; Maheshwari, D. K. Isolation of siderophore producing strains of Rhizobium meliloti and their biocontrol potential against Macrophomina phaseolina that causes charcoal rot of groundnut. Curr Sei, v. 81, n. 6, p. 673-677, 2001.
Arras G. & Arru S. Mechanism of action of some microbial antagonists against fungai pathogens. Annali di Microbiologia ed Enzimologia 47:97-120. 1997.
Baker, C.J.; Stavely, J.R.; Thomas, C.A.; Sasser, M. & Macfall, J.S. Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves Phaseolus vulgaris. Phytopathology, St. Paul, 73: 1148-1152, 1983.
Bernardo, E.R.A. Eficácia do controle biológico de Rotylenchus reniformis (Linford & Oliveira, 1940) em algodoeiro ( Gossypium hirsutum L.) com fungos nematófagos. Dissertação (Dissertação em Agronomia) - UNESP, Jaboticabal, 74p. 2002.
Bernardo, E.R.A. Controle da mancha preta dos frutos cítricos em cultivo orgânico e convencional e do bolor verde em pós -colheita. Dissertação em Agronomia) - Unesp, Botucatu, 72p. 2007.
Bettiol, W.; Ghini, R.; Morandi, M.A.B.; Stadnik, M.J.; Kraus, U.; Stefanova, M.; Prado, A.M.C. 2008. Controle biológico de doenças de plantas na América Latina. In: Alves, S.B. & Lopes, R.B. (Eds.) Controle Microbiano de Pragas na América Latina - Avanços e desafios. Piracicaba. FEALQ. 2008. pp. 303-331.
Bird D.M.; Opperman, C.H.; Davies K.G. 2003. Interactions between bactéria and plant-parasitic nematodes: now and then. Int. J. Parasitol. 33: 1269-1276. Cook, R.J.; Baker, K.F. 1983. The nature and practice of biological control ofplant pathogens. St. Paul: APS, 1983. 539p.
Crawford, D. Isolation and characterization of actinmycete antagonists of a fungai root pathogen. Applied and environmetal microbiology, Washington, v.59, n.l l, p.3899-3905. 1993.
Da Silva, G.J.; Vieira, J.D.; Soares, L.F.; Barros J.F,; Mendes, S.P.S.C.; Geraldine, A.M. Chemical and biological control on Pratylenchus brachyurus godfrey in soybean crop management. 50° congresso Brasileiro de fitopatologia. 2007.
De Mattos, L.P.V. Controle de Guignardia citricarpa e Penicillium digitatum em laranja com óleos essenciais e agentes de biocontrole. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Agronómicas, 2010. Esposito, E.; Paulilo, S.M.; Manfio, G. P. Biodegradation of the herbicide diuron by indigenous actinomycetes. Chemsphere, New York, v.37, p.541- 548, 1998.
Groth, I. Actinomycetes in Karstic caves af northem Spain (Altamira and Tito Bustillo). Journal of microbiological methods. Amsterdam, v.36, p.116-122, 1999.
Hallmann, J.; Faupel, A.; Krechel, A.; Sikora, R. A.; Berg, G. Endophytic bactéria and biological control of nematodes. Bulletin OILB/SROP. 27:83-94. 2004.
Hasky-Gíinther, K.; Hoffmann-Hergarten, S.; Sikora, R.A. Resistance against the potato cyst nematode Globodera pallida systemically induced by the rhizobacteria Agrobacterium radiobacter (G12) and Bacillus sphaericus (B43). Fund. Appl. Nematol. 21:511-517. 1998.
Heuer, H. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-eletrophoretic separation in denaturing gradientes. Applied and environmental microbiology, Washington, v.63, n.8, p.3233-3241, 1997.
Higaki, W.A. 2012. Bacillus subtilis e abamectina no controle de Rotylenchulus reniformis e Pratylenchus brachyurus e alterações fisiológicas em algodoeiro em condições controladas. Dissertação (Mestrado em Agronomia) - Universidade do Oeste Paulista - Unoeste: Presidente Prudente - SP.
Huang, T. & Chang, M. Studies on xanthobacidin, a new antibiotic from Bacillus subtilis active against Xanthomonas. Botanical Bulletin Academia Sinica 16: 137-148. 1975.
Hussain, M. B. Zahir, A. Z.; Asghar, H. N.; Mubaraka, R.; Muhammad, N. Potential of Rhizobium spp. for improving growth and yield of rice (Oryza sativa L.). Soil Environ, v. 28, n. 1, p. 49-55, 2009.
Kennedy, A.C. Bacterial diversity in agroecosystems. Agriculture ecossystems & environment. Amsterdam, v.74, p.65-76,1999.
Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular biology and evolution, p. msw054, 2016.
Motomura, M.; Hirooka, E. Y. Método rápido para o isolamento de microrganismos de solo com atividade antifúngica sobre Fusarium moniliforme. Arquivos de Biologia e Tecnologia, Curitiba, v. 39, n. 2, p. 313- 322, 1996.
Padilha, G. Biologia molecular de Streptomyces e aplicações industriais. In: Melo, I. S. & Azevedo, J. L. Ecologia Microbiana. Jaguariúna: Embrapa, 1998. p.327-347.
Pandhare, J.; Zog, K.; Deshpande, V. V. Deffereintial stabilities of alcaline protease inhibitors from actinomycetes: effect of varius additives on thermostability. Bioresource technology. Amsterdam, v.84, p.165-169, 2002. Schrijver, A. & Mot, R. Degradation of pesticides by actinomycetes. Criticai Reveiews in microbiologiy, Boca Raton, v.25, p.85-119, 1999.
Sikora, R.A & Hoffmann-Hergaten, S. Importance of plant health-promoting rhizobacteria for the control of soil-bome fungai diseases and plant parasitic nematodes. Arab. J. Plant Prot. 10:48-53. 1992.
Sikora, R.A. & Padgham, J.L. Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Crop Protection 26:971-977. 2007.

Claims

REIVINDICAÇÕES
1 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, caracterizada por compreender:
1,0 a 90,0% de pelo menos 2 (duas) espécies de Bacillus ou 2 (duas) ou mais cepas de uma mesma espécie e seus mutantes;
1,0 a 90,0 % de pelo menos 2 (dois) representantes de fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales e seus mutantes ou 2 (duas) ou mais cepas de uma mesma espécie de representantes das ordens; Bacillus em misturas com fungos das ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas), óleos essenciais de plantas ou extratos de plantas;
5,0 a 10,0% de defensivos agrícolas;
5,0 a 20,0% de óleo essencial;
5,0 a 20,0% de extrato de plantas;
1,0 a 15,0% de Aditivos; e
1,0 a 97,0% de Excipientes.
2 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1 , caracterizado pelos Bacillus da ordem Bacilales serem do grupo que consistem nas espécies de Bacillus velezensis, Bacillus aryabhattai, Bacillus genus, Bacillus pseudomycoides, Bacillus mucilaginosus, Bacillus sonorensis, Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus licheniformis, Bacilus paralicheniformis, Bacillus amyloliquefasciens, Bacillus megaterium, Bacillus sphaericus, Bacillus thuringiensis e qualquer outro gênero ou espécie da ordem supra citada.
3 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos fungos das ordens Hypocreales serem do grupo que consistem nas espécies de Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamomeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, Trichoderma virens, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora, Clonostachys farinosa, Clonostachys pseudobotrytis, Clonostachys pulvinata, Clonostachys rosea, (Link) Schroers, Clonostachys spectabilis, Clonostachys theobromae, Purpuriocillium lilacinus, Pochonia bulbillosa, Pochonia chlamydosporia, Pochonia chlamydosporia var. catenulata, Pochonia globispora, Pochonia goniodes, Pochonia humicola, Pochonia microbactrospora, Pochonia parasítica, Pochonia rubescens, Pochonia suchlasporia, Metarhizium anisopliae, Metarhizium acridum, Metarhizium brunneum, Metarhizium flavoviride, Metarizium rileyi, Aschersonia cubensi, Aschersonia aleyrodis, Aschersonia australiensis Aschersonia placenta, Isaria fumosorosea, Lecanicillium lecanni, Lecanicillium longisporum, Lecanicillium muscarium. Lecanicillium fungicola , Cordyceps militaris, Harposporium anguillulae e qualquer outro gênero ou espécie da ordem supra-citada.
4 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos fungos da ordem Orbiliales serem do grupo que consistem nas espécies de Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conoides, Arthrobotrys constringens, Arthrobotrys cylindrospora, Arthrobotrys dactyloides, Arthrobotrys elegans Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Arthrobotrys irregulares, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora, Dactylaria haptotyla, Dactylaria. purpurella, Dactylaria scaphoides, Dactylaria brochopaga, Dactylaria candida e qualquer outro gênero ou espécie da ordem supra-citada.
5 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos fungos da ordem Helotiales serem do grupo que consistem nas espécies de Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum, Monacrosporium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum, Monacrosporium cionopagu, Dactylella alba, Dactylella aphrobrocha, Dactylella arcuata, Dactylella arnaudii, Dactylella atractoides, Dactylella attenuata, Dactylella candida, Dactylella cionopaga, Dactylella fusiformis, Dactylella heterospora, Dactylella intermédia, Dactylella oxyspora, Dactylella ramosa e qualquer outro gênero ou espécie da ordem supra-citada. 6 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos fungos da ordem Mucorales serem do grupo que consiste na espécie de Cunninghamella elegans e qualquer outro gênero ou espécie da ordem supra citada.
7 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos defensivos serem compostos pelos seguintes princípios ativos:
a) Inseticidas - capsaicin, cartap, celastrus-extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, chlorpyrifos-e, cnidiadin, cryolite, cyanophos, cyantraniliprole, cyclaniliprole, cyhalothrin, cyhexatin, cypermethrin, dacnusa, DCIP, dichloropropene, dicofol, diglyphus, diglyphus+dacnusa, dimethacarb, dithioether, dodecylacetate, emamectin, encarsia, EPN, eretmocerus, ethylene-dibromide, eucalyptol, fatty-acids, fatty-acids/salts, fenazaquin, fenobucarb (BPMC), flubrocythrinate, flufenzine, formetanate, formothion, furathiocarb, gamma-cyhalothrin, vírus da granulose, NPV, ácido indol-3-ylbutyric, iodomethane, isocarbofos, isofenphos, isofenphos-m, isoprocarb, isothioate, kaolin, lindane, liuyangmycin, mephosfolan, metaldehyde, methamidophos, metolcarb (MTMC), óleo mineral, m-isothiocyanate, monosultap, oleic-acid, omethoate, orius, oxymatrine, paecilomyces, parathion-e, pasteuria, pheromones, phosphorus-acid, photorhabdus, phoxim, phytoseiulus, pirimiphos-e, vírus da poliedrose, , oleato de potassium, prothiofos, pyraclofos, pyrethrinas, pyridaphenthion, pyrimidifen, pyriproxifen, sulfluramid, tebupirimfos, tefluthrin, temephos, tetradifon, thiofanox, thiometon, triazamate, triflumuron, vertrine, kappa-bifenthrin, kappa-tefluthrin, dichoromezotiaz, broflanilide, pyraziflumid aldicarb, alanycarb, benfuracarb, carbaryl, carbofuran, carbosulfan, methiocarb, methomyl, oxamyl, pirimicarb, propoxur e thiodicarb, acephate, azinphos-ethyl, azinphos-methyl, chlorfenvinphos, chlorpyrifos, chlorpyrifos-methyl, demeton-Smethyl, diazinon, dichlorvos/DDVP, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidaphos, methidathion, mevinphos, monocrotophos, oxymethoate, oxydemeton-methyl, parathion, parathion- methyl, phenthoate, phorate, phosalone, phosmet, phosphamidon, pirimiphos- methyl, quinalphos, terbufos, tetrachlorvinphos, triazophos, trichlorfon, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, spinosad, spinetoram, mectins, abamectin, emamectin benzoate, ivermectin, lepimectin, milbemectin, hydroprene, kinoprene, methoprene, fenoxycarb, pyriproxyfen; pymetrozine, flonicamid, pyrifluquinazon, clofentezine, hexythiazox, etoxazole, diafenthiuron, fenbutatin oxide, propargite, chlorfenapyr, bensultap, cartap hydrochloride, thiocydam, thiosultap sodium, bistrifluron, diflubenzuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, teflubenzuron, buprofezin, cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, amitraz, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim, cyenopyrafen, cyflumetofen, hydramethylnon, acequinocyl, fluacrypyrim, indoxacarb, metaflumizone, spirodiclofen, spiromesifen, spirotetramat, flubendiamide, chlorantraniliprole, cyclaniliprole, cyantraniliprole, azadirachtin, amidoflumet, bifenazate, fluensulfone, piperonyl butoxide, pyridalyl, sulfoxaflor, acrinathrin, allethrin, bifenthrin, cyfluthrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, tau-fluvalinate, permethrin, silafluofen e tralomethrin, dentre outros;
b) Fungicidas: benzovindiflupyr, antiperonosporic, ametoctradin, amisulbrom, hidróxido de cobre, oxychloride de cobre, cobre, sulfate, persulfate de cobre, boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, isofetamid, fenfuram, bixafen, fluxapyroxad, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyrametostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, fentin hydroxide, oxytetracycline, chlozolinate, chloroneb, tecnazene, etridiazole, iodocarb, prothiocarb, pyrisoxazole, oxpoconazole, etaconazole, fenpyrazamine, fenpicoxamide, mefentrifluconazole, naftifine, terbinafine, validamycin, pyrimorph, valifenalate, fthalide, probenazole, isotianil, laminarin, teclofthalam, triazoxide, pyriofenone, chlorothalonil, fluoroimide, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxiconazole, fluquinconazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, simeconazole, triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamid, benomyl, carbendazim, thiabendazole, fuberidazole, ethaboxam, etridiazole and hymexazole, azaconazole, diniconazole-M, oxpoconazol, paclobutrazol, uniconazol, l-(4- chloro-phenyl)-2-([l,2,4]triazol-lyl)-cycloheptanol, imazalilsulfphate, azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, methominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, enestroburin, methyl (2-chloro-5-[l-(3- methylbenzyloxyimino)ethyl]benzyl)carbamate, methyl (2-chloro-5-[l-(6- methylpyridin-2- ylmethoxyimino)ethyl]benzyl)carbamate, methyl 2-(ortho- (2,5-dimethylphenyloxymethylene)- phenyl)-3-methoxyacrylate, 2-(2-(6-(3- chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)- phenyl)-2- methoxyimino-N-methyl-acetamide, 3-methoxy-2-(2-(N-(4-methoxy-phenyl)- cyclopropanecarboximidoylsulfanylmethyl)-phenyl)-acrylic acid methyl ester; carboxin, benalaxyl, benalaxyl-M, fenhexamid, flutolanil, furametpyr, mepronil, metalaxyl, mefenoxam, ofurace, oxadixyl, oxycarboxin, penthiopyrad, isopyrazam, thifluzamide, tiadinil, 3,4-dichloro-N-(2- cyanophenyl)isothiazole-5-carboxamide, dimethomorph, flumorph, flumetover, fluopicolide (picobenzamid), zoxamide, carpropamid, diclocymet, mandipropamid, N- (2-(4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3- methoxyphenyl)ethyl)-2-methanesulfonyl-amino-3- methylbutyramide, N-(2- (4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3-methoxy-phenyl)ethyl)-2- ethanesulfonylamino- 3 -methylbutyramide, methyl 3-(4-chlorophenyl)-3-(2- isopropoxycarbonylamino-3-methyl-butyrylamino)propionate, N-(4'- bromobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N- (4'-trifluoromethyl-biphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5- carboxamide, N-(4'- chloro-3'-fluorobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-3- difluoro-methyl-l-methylpyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-3-difluoromethyl-lmethylpyrazole-4-carboxamide, N-(2- cyano-phenyl)- 3,4-dichloroisothiazole-5-carboxamide, 2- amino-4-methyl- thiazole-5-carboxanilide, 2-chloro-N-(l,l,3-trimethyl-indan-4-yl)- nicotinamide, N- (2-(l,3-dimethylbutyl)-phenyl)-l,3-dimethyl-5-fluoro-lH- pyrazole-4-carboxamide, N-(4'-chloro3',5-difluoro-biphenyl-2-yl)-3- difluoromethyl-l-methyl-IH-pyrazole-4-carboxamide, N-(4'-chloro3',5- difluoro-biphenyl- 2-yl)-3-trifluoromethyl-l -methyl-lH-pyrazole-4- carboxamide, N-(3',4'- dichloro-5-fluoro-biphenyl-2-yl)-3-trifluoromethyl-l- methyl-lH-pyrazole-4- carboxamide, N-(3',5difluoro-4'-methyl-biphenyl-2- yl)-3-difluoromethyl- 1 -methyl-lH-pyrazole-4-carboxamide, N- (3',5- difluoro-4'-methyl-biphenyl-2-yl)-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N- (cis-2-bicyclopropyl-2-yl-phenyl)-3-difluoromethyl-l methyl-lH-pyrazole-4-carboxamide, N- (trans-2-bicyclopropyl-2-yl-phenyl)- 3-difluoro-methyl-l-methyl-l H-pyrazole-4-carboxamide, fluopyram, N-(3- ethyl-3,5-5- trimethyl-cyclohexyl)-3-formylamino-2-hydroxy-benzamide, oxytetracyclin, silthiofam, N-(6-methoxy-pyridin-3-yl) cyclopropanecarboxamide, 2-iodo-N-phenylbenzamide, N-(2-bicyclo-propyl- 2-yl-phenyl)-3-difluormethyl-l-methylpyrazol-4-ylcarboxamide, N- (3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethyl-5-fluoropyrazol-4-yl-carboxamide, N- (3',4',5'-trifluorobiphenyl-2-yl)-5-chlorol,3-dimethyl- pyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl- lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-lmethylpyrazol-4-ylcarboxamide,N-(3',4',5'- trifluorobiphenyl-2-yl)-3 -difluoromethyl-lmethylpyrazol-4-ylcarboxamide, N - (3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-lmethylpyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-l - methylpyrazol-4-ylcarboxamide, N- (3', 4', 5'-trifluorobiphenyl-2-yl)-3- (chlorodifluoromethyl)-lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5- chloro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)- 1 ,3-dimethyl-5-fluoropyrazol-4-ylcarboxamide, N- (2, ,4 , ,5'- trifluorobiphenyl-2-yl)-5-chloro-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-fluoromethyl-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-l-methylpyrazol-4-ylcarboxamide,N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2- yl)-5-chloro- 3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N- (2',4',5'- trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-l-methylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5 trifluorobiphenyl-2-yl)- 5-fluoro-l-methyl-3-trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-5-chloro-l-methyl-3-trifluoromethylpyrazol-4- ylcarboxamide, N-(3',4'- dichloro-3-fluorobiphenyl-2-yl)-l -methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'- dichloro-3- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-l H-pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl- S-difluoromethyl-1 H-pyrazole-4-carboxamide, N-(3'- chloro-4'-fluoro-3- fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3A4'-dichloro-4-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-difluoro-4-fluorobiphenyl-2-yl)-l - methyl- S-trifluoromethyl-lH-pyrazole-4-carboxamide, N- (3 ',4'-dichloro-4- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4- carboxamide, N- (3M'-difluoro-4-fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH- pyrazole-4-carboxamide, N- (3'-chloro-4'-fluoro-4-fluorobiphenyl-2-yl)-l- methyl-5-difluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-l - methyl- S-difluoromethyl-IH-pyrazole-carboxamide, N- (3',4'-difluoro-5- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3',4'-dichloro-5-fluorobiphenyl-2-yl)-l,3-dimethyl-l H-pyrazole-4- carboxamide, N-(3'-chloro-4'- fluoro-5-fluorobiphenyl-2-yl)-l-methyl-3- difluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-4-fluorobiphenyl-2- yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'-fluoro- 5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide,N-(4'- chloro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl- lH-pyrazole-4-carboxamide, N-(4'- methyl-5-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-5-fluorobiphenyl- 2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-chloro-5- fluorobiphenyl-2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-methyl- 5-fluorobiphenyl-2- yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4- carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-[2-(l, 1,2, 3,3,3- hexafluoropropoxy)-phenyl]-3- difluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]- 3-difluoromethyl-l- methyl-lH-pyrazole-4-carboxamide and N-[4'-(trifluoromethylthio)- biphenyl2-yl]-l-methyl-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, fluazinam, pyrifenox, bupirimate, cyprodinil, fenarimol, ferimzone, mepanipyrim, nuarimol, pyrimethanil, triforine, fenpiclonil, fludioxonil, aldimorph, dodemorph, fenpropimorph, tridemorph, fenpropidin, iprodione, procymidone, vinclozolin, famoxadone, fenamidone, octhilinone, probenazole, 5-chloro-7-(4-methyl-piperidin-l-yl)-6-(2,4,6- trifluorophenyl)-
[1.2.4]triazolo[l,5-a]pyrimidine, anilazine, diclomezine, pyroquilon, proquinazid, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, acibenzolar-S-methyl, captafol, captan, dazomet, folpet, fenoxanil, quinoxyfen, N,N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-lsulfonyl)-
[1.2.4]triazole-l-sulfonamide, 5-ethyl-6-octyl-[l,2,4]triazolo[l,5- a]pyrimidin-
2,7-diamine, 2,3,5,6-tetrachloro-4-methanesulfonyl-pyridine, 3,4,5-trichloro- pyridine-2,6-di-carbonitrile, N-(l- (5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2,4- dichloro-nicotinamide, N-((5-bromo-3-chloropyridin-2- yl)-methyl)-2,4- dichloro-nicotinamide, diflumetorim, nitrapyrin, dodemorphacetate, fluoroimid, blasticidin-S, chinomethionat, debacarb, difenzoquat, difenzoquat-methylsulphat, ácido oxolinic, piperalin, mancozeb, maneb, metam, methasulphocarb, metiram, ferbam, propineb, thiram, zineb, ziram, diethofencarb, iprovalicarb, benthiavalicarb, propamocarb, propamocarb hydrochlorid, 4-fluorophenyl N-(l-(l-(4-cyanophenyl)-ethanesulfonyl)but-2- yl)carbamate, methyl 3-(4-chloro-phenyl)-3-(2- isopropoxycarbonylamino-3- methylbutyrylamino) propanoate, guanidine, iminoctadine, guazatine, kasugamycin, oxytetracyclin, streptomycin, polyoxin, validamycin, binapacryl, dinocap, dinobuton, sulfur-containing heterocyclyl compounds: dithianon, isoprothiolane, edifenphos, iprobenfos, fosetyl, fosetyl-aluminum, pyrazophos, tolclofos- methyl, dichlofluanid, flusulfamide, hexachloro- benzene, phthalide, pencycuron, quintozene, thiophanate, thiophanatemethyl, cyflufenamid, cymoxanil, dimethirimol, ethirimol, furalaxyl, metrafenone and spiroxamine, guazatine-acetate, iminoc- tadine-triacetate, iminoctadinetris(albesilate), kasugamycin hydrochloride hydrate, dichlorophen, pentachlorophenol and its salts, N-(4- chloro-2-nitro-phenyl)- N-ethyl-4-methyl-benzenesulfonamide, dicloran, nitrothal-isopropyl, tecnazen, biphenyl, bronopol, diphenylamine, mildiomycin, oxincopper, prohexadione calcium, N - (cyclopropylmethoxyimino- (6-difluoromethoxy- 2,3-difluoro-phenyl)-methyl)-2-ph acetamide, N'-(4-(4-chloro-3- trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl
formamidine, N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl- phenyl)-N-ethyl-N-methyl formamidine, N'-(2-methyl-5-trifluonnethyl-4-(3- trimethylsilanyl-propoxy)-phenyl)-N-ethyl-Nmethylformamidine and N'-(5- difluormethyl-2-methyl- 4-(3-trimethylsilanyl-propoxy)-phenyl)-Nethyl-N- methyl formamidine;
c) Herbicidas: alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; éster de phenoxyphenoxypropionic, clodinafop-propargyl, cyhalofop-butyl, diclofop- methyl, fenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop- butyl, fluazifop-P-butyl, haloxyfop-ethoxyethyl, haloxyfop-methyl, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl ou quizalofop-tefuryl, flamprop-methyl, flamprop- isopropyl, imazapyr, imazaquin, imazamethabenz-methyl (imazame), imazamox, imazapic, imazethapyr, pyrithiobac-acid, pyrithiobac-sodium, bispyribac-sodium, pyribenzoxym, florasulam, flumetsulam, metosulam, amidosulfuron, azimsulfuron, bensulfuron-methyl, chlorimuron-ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron-methyl, ethoxysulfuron, flazasulfuron, halosulfuron-methyl, imazosulfuron, metsulfuron-methyl, nicosulfuron, primisulfuron-methyl, prosulfuron, pyrazosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, thifensulfuron- methyl, triasulfuron, tribenuron-methyl, triflusulfuron-methyl, tritosulfuron, sulfosulfuron, foramsulfuron, iodosulfuron, benzoyl prop-ethyl, bromobutide, chiorthiamid, diphenamid, etobenzanidibenzchlomet, fluthiamide, fosamin, monalide, clopyralid, picloram, 2,4-D, benazolin, naptalame, diflufenzopyr, benzofenap, clomazone, diflufenican, fluorochloridone, fluridone, pyrazolynate, pyrazoxyfen, isoxaflutole, isoxachlortole, mesotrione, sulcotrione, ketospiradox, flurtamone, norflurazon, amitrol, glyphosate, sulfosate, bilanafos, glufosinateammonium, anilofos, mefenacet, dimethenamid, S-dimethenamid, acetochlor, alachlor, butachlor, butenachlor, diethatyl-ethyl, dimethachlor, metazachlor, metolachlor, S-metolachlor, pretilachlor, propachlor, prynachlor, terbuchlor, thenylchlor, xylachlor, butylate, cycloate, di-allate, dimepiperate, EPTC. esprocarb, molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, tiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butamifos, chlorthal-dimethyl (DCPA), acifluorfen, acifluorfen-sodium, aclonifen, bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl, oxadiazon, azafenidin, butafenacil, carfentrazone- ethyl, cinidon-ethyl, flumiclorac-pentyl, flumioxazin, flumipropyn, flupropacil, fluthiacet-methyl, sulfentrazone, thidiazimin, nipyraclofen, propanil, pyridate or pyridafol, bromofenoxim, dinoseb, dinoseb-acetate, dinoterb, cyperquat-chloride, difenzoquat-methylsulfate, diquat or paraquatdichloride, chlorbromuron, chlorotoluron, difenoxuron, dimefuron, diuron, ethidimuron, fenuron, fluometuron, isoproturon, isouron, linuron, methabenzthiazuron, methazole, metobenzuron, metoxuron, monolinuron, neburon, siduron, tebuthiuron, bromoxynil, ioxynil, chloridazon, ametryn, atrazine, cyanazine, desmein, dimethamethryn, hexazinone, prometon, prometryn, propazine, simazine, simetryn, terbumeton, terbutryn, terbutylazine, trietazine, metamitron, metribuzin bromacil, lenacil, terbacil, desmedipham, phenmedipham, tridiphane, isoxaben, dichlobenil, dalapon, ethofumesate, chlorfenac, aziprotryn, barban, bensulide, benzthiazuron, benzofluor, buminafos, buthidazole, buturon, cafenstrole, chlorbufam, chlorfenprop-methyl, chloroxuron, cinmethylin, cumyluron, cycluron, cyprazine, cyprazole, dibenzyluron, dipropetryn, dymron, eglinazin-ethyl, endothall, ethiozin, flucabazone, fluorbentranil, flupoxam, isocarbamid, isopropalin, karbutilate, mefluidide, monuron, napropamide, napropanilide, nitralin, oxaciclomefone, phenisopham, piperophos, procyazine, profluralin, pyributicarb, secbumeton, sulfallate (CDEC), terbucarb, triaziflam, triazofenamid, trimeturon, dentre outros;
d) Nematicidas: Benomyl, cloethocarb, aldoxycarb, tirpate, diamidafos, fenamiphos, cadusafos, dichlofenthion, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofof, isazofos, phosphocarb, thionazin, imicyafos, mecarphon, acetoprole, benclothiaz, chloropicrin, dazomet, fluensulfone, 1,3-dichloropropene (telone), dimethyl disulfide, metam sodium, metam potassium, methyl bromide, allyl isothiocyanate (AITC), dimethyl sulfate, furfual (aldehyde), dentre outros.
8 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos óleos essenciais e extratos vegetais obtidos de sementes, plantas e parte de plantas de Ricinus communis, Crotalaria juncea, Chenopodium ambrosioides, Azadirachta indica, Verbena ojficinalis, Erythrina mulungu, Quassia amara, Bidens pilosa, Plantago lanceolata, Senecio brasiliensis, Lantana camara, Tagetes patula, Lantana montevidensis, Eucalyptus spp., Lantana montevidensis, Lantana câmara, Senecio brasiliensis, Chrysantemum coronarium, Solanum americanum, Solanum paniculatum Hovenia dulcis, Melia azedarach, Butia eriospatha, Schinus terenbithifolius, Cinnamomum camphora, Conyza bonariemis, Senecio brasiliensis, Bidens pilosa, Amaranthus hybridus, Euphorbia heterophylla, Raphanus sativus, Ipomoea purpurea, Brachiaria plantaginea, Ruta graveolens, Aloysia triphylla, Brassica napus, Mucuna pruriens, Ocimum gratissimum, Lippia sidoides, Croton zehntneri, Mentha piperita, Cymbopogon martinii, Cymbopogon schoenanthus, Piper aduncum, Arisaema franchetianum, Zanthoxylum simulans, Ruta chalepensis, Citrus sinensis, Melaleuca quinquenervia, Cymbopogon citratus, Thymus vulgaris, Piper aduncum, Melaleuca alternifolia, Hesperozygis myrtoides e demais espécies dos gêneros supra citados.
9 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos aditivos serem dispersantes selecionados do grupo consistindo de polímeros iônicos solúveis em água, aminoácidos, extratos de algas, compostos de origem natural, polímeros aniônicos solúveis em água, surfactantes selecionados do grupo consistindo de surfactantes aniônicos e surfactantes não iônicos e combinações entre eles e derivados.
10 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos excipientes serem do grupo que consiste em sílicas, talco, bentonita, carboidratos, açúcares, amidos, carbonatos, derivados do leite (soro e leite em pó) e combinações entre eles e derivados.
11 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada como formulação suspensão de encapsulado, concentrado dispersível, concentrado emulsionável, emulsão de água em óleo, emulsão de óleo em água, microemulsão, suspensão concentrada, suspo-emulsão, granulado solúvel, concentrado solúvel, pó solúvel, tablete, granulado dispersível, pó molhável, gel emulsionável, gel solúvel em água, grânulo emulsionável, pó emulsionável, dispersão de óleo ou suspensão concentrada em óleo, formulação mista de concentrado solúvel e susensão concetrada, formulação mista de concentrado sóluvel e suspo-emulsão, formulação mista de concentrado solúvel e emulsão de óleo em água, suspensão concentrada dispersível ou miscível em óleo, solução miscível em óleo, pó dispersível em óleo, granulado encapsulado, pó seco, líquido para pulverização eletrostática/eletrodinâmica, granulado, óleo para pulverização/espalhamento, suspensão a ultrabaixo volume, ultrabaixo volume, microgranulado, pó fino, granulado fino, macrogranulado, encapsulamento, microencapsulamento e nanoencapsulamento.
12 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada como pulverização, pulverização via barra, pulverização aérea, baixo volume, ultra baixo volume, pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, não se limitando a Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp. , Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, crotalárias ( Crotalaria juncea, Crotalaria spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora ) e demais espécies dos gêneros supra-citados.
13 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de fitopatógenos, tais como: Phakopsora pachyrhizi, Corynespora cassiicola, Puccinia polysora, Exserohilum turcicum, Xanthomonas spp. ( Xanthomonas axonopodis, Xanthomonas Campestis, Xanthomonas vesicatória ), Ramularia areola, Hemileia vastatrix, Cercospora coffeicola, Phoma spp. ( Phoma costaricensis ), Ascochyta spp. ( Ascochyta coffeae ), Pseudomonas spp. {Pseudomonas syringae, Pseudomonas corrugata ), Rhizoctonia solani, Fusarium spp. ( Fusarium solani, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum), Colletotrichum spp. ( Colletotrichum gloeosporioides, Colletotrichum truncatum, Colletotrichum lindemuthianum ) Macrophomina phaseolina, Sclerotinia sclerotiorum, Botrytis cinerea, Penicillium digitatum, e demais espécies dos gêneros supra-citados.
14 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de fitonematóides, tais como: Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incógnita, Meloidogyne javanica, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Meloidogyne exigua, Helicotilechus dihystera, Meloidogyne exigua, Meloidogyne paranaensis , Meloidogyne coffeicola, Meloidogyne ha pia, Meloidogyne enterolobii, Meloidogyne arabicidae, Meloidogyne arenaria, Pratylenchus brachiurus, Pralylenchus coffea, Pratylenchus zeae Xiphinema krugi, Heterodera glycines, Helicotylenchus sp., Paratrichodorus sp., Tylenchorhynchus sp., Scutellonema sp., Aphelenchoides spp., Aphelenchus spp., Mesocriconema sp., Tylenchus sp. e demais espécies dos gêneros supra citados.
15 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de pragas agrícolas (insetos e ácaros), tais como: Polyphagotarsonemus latus, Tetranychus urticae, Diabrotica speciosa, Anticarsia gemmatalis, Spodoptera spp. ( Spodoptera cosmioides, Spodoptera frugiperda ), Pseudoplusia includens, Trichoplusia ni, Bemisia tabaci, Euschistos heros, Nezara viridula, Leucoptera coffeella, Hypothenemus hampei, Oligonychus ilicis, Polyphagotarsonemus latus , Brevipalpus phoenicis, Planococcus citri, Planococcus minor, Helicoverpa armígera e demais espécies dos gêneros supra-citados.
16 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de bioestimulantes, inoculantes, solubilizador de fósforo e estimulantes de crescimento nas plantas cultivadas, tais como: Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta ), Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.), Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Crotalaria spp. ( Crotalaria juncea, , Crotalária spectabilis,
Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora ) e demais espécies dos gêneros supra-citados.
17 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de condicionador de solos para todas as culturas e qualquer área agriculturável.
18 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de mitigar ou reduzir o estresse hídrico ou abiótico nas plantas cultivadas, tais como: Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Crotalaria spp. (Crotalaria juncea, 5 Crotalária spectabilis, Crotalaria ochroleuca,
Crotalaria paulina, Crotalaria breviflora), dentre outras.
19 - COMPOSIÇÕES BIOLÓGICAS DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de biorremediador de áreas agricultáveis ou não para a redução de contaminação ambiental por resíduos tóxicos.
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110452848A (zh) * 2019-08-20 2019-11-15 昆明理工大学 一株贝莱斯芽孢杆菌及其应用
CN112410268A (zh) * 2020-12-09 2021-02-26 甘肃省农业科学院植物保护研究所 一株副地衣芽孢杆菌及其应用
CN114304188A (zh) * 2021-12-15 2022-04-12 宁夏大学 一种蜡蚧轮枝菌可分散油悬浮剂及其制备方法与应用
WO2022154653A1 (es) * 2021-01-14 2022-07-21 Becerra Carranza Luis Rodrigo Mezcla, formulación plaguicida y método, para controlar plagas de semillas y granos vegetales almacenados
WO2022191691A1 (es) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo CEPA AISLADA DE Trichoderma Harzianum, MEZCLA Y MÉTODO, PARA PROMOVER LA GERMINACIÓN DE SEMILLAS VEGETALES
WO2022191692A1 (es) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo CEPA DE Bacillus Subtilis, MEZCLAS Y MÉTODO, PARA EL CONTROL DE HONGOS FITOPATÓGENOS
WO2022191694A1 (es) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo CEPA DE Azospirillum Brasilense, MEZCLAS Y MÉTODO, PARA PROMOVER EL DESARROLLO RADICULAR DE PLÁNTULAS VEGETALES
WO2023219483A1 (es) * 2022-05-09 2023-11-16 Becerra Carranza Luis Rodrigo Mezcla probiótica, biofungicida y método, para el control de hongos micotoxigénicos
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* Cited by examiner, † Cited by third party
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WO2023233182A1 (es) * 2022-05-31 2023-12-07 Agroadvance Spa Inoculante biológico con acción biocontroladora contra xanthomona arboricola
CN115161239B (zh) * 2022-07-25 2023-10-24 四川龙蟒福生科技有限责任公司 一种bacillus velezensis菌的专用发酵培养基及其应用
CN115161240B (zh) * 2022-07-25 2023-10-24 四川龙蟒福生科技有限责任公司 一种bacillus velezensis菌株及其应用
CN115125175B (zh) * 2022-07-25 2023-10-24 四川龙蟒福生科技有限责任公司 一种杀螨菌株的发酵方法及其应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194193B1 (en) 1998-12-11 2001-02-27 David J. Drahos Nutrient plant formulation with microbial strains
US6808917B1 (en) 2001-02-02 2004-10-26 Thomas D. Johnson Controlling plant pathogens with fungal/bacterial anatagonist combinations
US7429477B2 (en) 2001-02-02 2008-09-30 Johnson Thomas D Controlling plant pathogens with bacterial/fungal antagonist combinations
US20110110906A1 (en) 2008-04-07 2011-05-12 Bayer Cropscience Ag Combinations of biological control agents and insecticides or fungicides
WO2012020014A1 (en) * 2010-08-10 2012-02-16 Chr. Hansen A/S Nematocidal composition comprising bacillus subtilis and bacillus licheniformis
WO2018045063A1 (en) 2016-08-31 2018-03-08 Fmc Corporation Nematocidal compositions comprising bacillus licheniformis and bacillus subtilis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2485796C (en) * 2004-06-18 2013-08-20 Thomas D. Johnson Controlling plant pathogens with fungal/bacterial antagonist combinations comprising trichoderma virens and bacillus subtilis var.amyloliquefaciens
WO2010109436A1 (en) * 2009-03-25 2010-09-30 Carepro Bioscience (P) Ltd Microbial formulation for widespread uesd in agricultural practices
US9862922B2 (en) * 2015-09-17 2018-01-09 Polyorganic Technologies Corporation Rapid growth activator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194193B1 (en) 1998-12-11 2001-02-27 David J. Drahos Nutrient plant formulation with microbial strains
US6808917B1 (en) 2001-02-02 2004-10-26 Thomas D. Johnson Controlling plant pathogens with fungal/bacterial anatagonist combinations
US7429477B2 (en) 2001-02-02 2008-09-30 Johnson Thomas D Controlling plant pathogens with bacterial/fungal antagonist combinations
US20110110906A1 (en) 2008-04-07 2011-05-12 Bayer Cropscience Ag Combinations of biological control agents and insecticides or fungicides
WO2012020014A1 (en) * 2010-08-10 2012-02-16 Chr. Hansen A/S Nematocidal composition comprising bacillus subtilis and bacillus licheniformis
WO2018045063A1 (en) 2016-08-31 2018-03-08 Fmc Corporation Nematocidal compositions comprising bacillus licheniformis and bacillus subtilis

Non-Patent Citations (37)

* Cited by examiner, † Cited by third party
Title
ALAMI, Y.ACHOUAK WMAROL CHEULIN T: "Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp. Strain isolated from sunflower roots", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 8, 2000, pages 3393 - 3398
ARAUJO, F.F.: "Inoculagao de sementes com Bacillus subtilis, formulado com farinha de ostra e desenvolvimento de milho, soja e algodao", CIENCIAS E AGROTECNOLOGIA, LAVRAS, vol. 2, 2008, pages 456 - 462
ARAUJO, J. M.: "Microbiana", 1998, article "Estrategias para isolamento seletivo de Actinomicetos", pages: 327 - 347
ARORA, N. K.KANG, S. C.MAHESHWARI, D. K.: "Isolation of siderophore producing strains of Rhizobium meliloti and their biocontrol potential against Macrophomina phaseolina that causes charcoal rot of groundnut", CURR SEI, vol. 81, no. 6, 2001, pages 673 - 677
ARRAS G.ARRU S.: "Mechanism of action of some microbial antagonists against fungal pathogens", ANNALI DI MICROBIOLOGIA ED ENZIMOLOGIA, vol. 47, 1997, pages 97 - 120, XP008089863
BAKER, C.J.STAVELY, J.R.THOMAS, C.A.SASSER, M.MACFALL, J.S.: "Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves Phaseolus vulgaris", PHYTOPATHOLOGY, vol. 73, 1983, pages 1148 - 1152
BERNARDO, E.R.A.: "Controle da mancha preta dos frutos citricos em cultivo organico e convencional e do bolor verde em pos-colheita", DISSERTAGAO EM AGRONOMIA) - UNESP, BOTUCATU, 2007, pages 72
BERNARDO, E.R.A.: "Eficacia do controle biologico de Rotylenchus reniformis (Linford & Oliveira, 1940) em algodoeiro (Gossypium hirsutum L.) com fungos nematofagos", DISSERTAGAO (DISSERTAGAO EM AGRONOMIA) - UNESP, J ABOTICABAL, 2002, pages 74
BETTIOL, W.GHINI, R.MORANDI, M.A.B.STADNIK, M.J.KRAUS, U.STEFANOVA, M.PRADO, A.M.C.: "Controle Microbiano de Pragas na America Latina - Avangos e desafios", 2008, article "Controle biologico de doengas de plantas na America Latina", pages: 303 - 331
BIRD D.M.OPPERMAN, C.H.DAVIES K.G.: "Interactions between bacteria and plant-parasitic nematodes: now and then", INT. J. PARASITOL., vol. 33, 2003, pages 1269 - 1276
COOK, R.J.BAKER, K.F., THE NATURE AND PRACTICE OF BIOLOGICAL CONTROL OF PLANT PATHOGENS, 1983, pages 539
CRAWFORD, D.: "Isolation and characterization of actinmycete antagonists of a fungal root pathogen", APPLIED AND ENVIRONMETAL MICROBIOLOGY, vol. 59, no. 11, 1993, pages 3899 - 3905, XP000600373
DA SILVA, G.J.VIEIRA, J.D.SOARES, L.F.BARROS J.FMENDES, S.P.S.C.GERALDINE, A.M.: "Chemical and biological control on Pratylenchus brachyurus godfrey in soybean crop management", 50° CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 2007
DE MATTOS, L.P.V.: "Tese (doutorado", 2010, UNIVERSIDADE ESTADUAL PAULISTA, FACULDADE DE CIENCIAS AGRONOMICAS, article "Controle de Guignardia citricarpa e Penicillium digitatum em laranja com oleos essenciais e agentes de biocontrole"
ESPOSITO, E.PAULILO, S.M.MANFIO, G. P.: "Biodegradation of the herbicide diuron by indigenous actinomycetes", CHEMSPHERE, vol. 37, 1998, pages 541 - 548
GALPER S ET AL.: "A Collagenolytic Fungus, Cunninghamella elegans, for Biological Control of Plant-parasitic Nematodes", J NEMATOL., vol. 23, no. 3, July 1991 (1991-07-01), pages 269 - 74, XP055747653 *
GROTH, L: "Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo", JOURNAL OF MICROBIOLOGICAL METHODS. AMSTERDAM, vol. 36, 1999, pages 116 - 122
HALLMANN, J.FAUPEL, A.KRECHEL, A.SIKORA, R. A.BERG, G.: "Endophytic bacteria and biological control of nematodes", BULLETIN OILB/SROP., vol. 27, 2004, pages 83 - 94
HASKY-GUNTHER, K.HOFFMANN-HERGARTEN, S.SIKORA, R.A.: "Resistance against the potato cyst nematode Globodera pallida systemically induced by the rhizobacteria Agrobacterium radiobacter (G 12) and Bacillus sphaericus (B43", FUND. APPL. NEMATOL., vol. 21, 1998, pages 511 - 517
HEUER, H.: "Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-eletrophoretic separation in denaturing gradients", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 8, 1997, pages 3233 - 3241, XP002956278
HIGAKI, W.A.: "Dissertagao (Mestrado em Agronomia", 2012, UNIVERSIDADE DO OESTE PAULISTA, article "Bacillus subtilis e abamectina no controle de Rotylenchulus reniformis e Pratylenchus brachyurus e a1terações fisiologicas em algodoeiro em condicoes controladas"
HUANG, T.CHANG, M.: "Studies on xanthobacidin, a new antibiotic from Bacillus subtilis active against Xanthomonas", BOTANICAL BULLETIN ACADEMIA SINICA, vol. 16, 1975, pages 137 - 148
HUSSAIN, M. B.ZAHIR, A. Z.ASGHAR, H. N.MUBARAKA, R.MUHAMMAD, N.: "Potential of Rhizobium spp. for improving growth and yield of rice (Oryza sativa L.", SOIL ENVIRON, vol. 28, no. 1, 2009, pages 49 - 55
KENNEDY, A.C.: "Bacterial diversity m agroecosystems", AGRICULTURE ECOSSYSTEMS & ENVIRONMENT, vol. 74, 1999, pages 65 - 76
KUMAR, SUDHIRSTECHER, GLENTAMURA, KOICHIRO: "MEGA 7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets", MOLECULAR BIOLOGY AND EVOLUTION, 2016, pages msw054
MASCIA, RODRIGO: "Bacillus amyloliquefaciens e Trichoderma harzianum no manejo de Pratylenchus brachyurus e Helicotylenchus sp. na cultura da soja", DISSERTAÇÃO DE MESTRADO, 13 March 2017 (2017-03-13), XP055659516 *
MIN LIAO ET AL.: "Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation", PLOS ONE, vol. 11, no. 12, 9 December 2016 (2016-12-09), pages e0167748, XP055747656 *
MOTOMURA, M.HIROOKA, E. Y.: "Metodo rapido para o isolamento de microrganismos de solo com atividade antifungica sobre Fusarium moniliforme", ARQUIVOS DE BIOLOGIA E TECNOLOGIA, CURITIBA, vol. 39, no. 2, 1996, pages 313 - 322
OWNLEY, B.H. ET AL.: "Beauveria-bassiana, a dual purpose biological control with activity against insect pests and plant pathogens", EMERGING CONCEPTS IN PLANT HEALTH, 2004, pages 255 - 269, XP055747651 *
PADILHA, G.: "Ecologia Microbiana", 1998, article "Biologia molecular de Streptomyces e ap1icações industriais", pages: 327 - 347
PANDHARE, J.ZOG, K.DESHPANDE, V. V.: "Differential stabilities of alcaline protease inhibitors from actinomycetes: effect of various additives on thermostability", BIORESOURCE TECHNOLOGY, vol. 84, 2002, pages 165 - 169
PEREZ-GARCIA, A. ET AL.: "Plant protection and stimulation by microorganisms: biotechnological applications of Bacilli in agriculture", CURRENT OPINION IN BIOTECHNOLOGY, vol. 22, 2011, pages 187 - 193, XP055236081, DOI: 10.1016/j.copbio.2010.12.003 *
SCHRIJVER, A.MOT, R.: "Degradation of pesticides by actinomycetes", CRITICAL REVIEWS IN MICROBIOLOGIY, vol. 25, 1999, pages 85 - 119
See also references of EP3954214A4
SIKORA, R.A.PADGHAM, J.L.: "Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice", vol. 26, 2007, CROP PROTECTION, pages: 971 - 977
SIKORA, R.AHOFFMANN-HERGATEN, S.: "Importance of plant health-promoting rhizobacteria for the control of soil-borne fungal diseases and plant parasitic nematodes", ARAB. J. PLANT PROT., vol. 10, 1992, pages 48 - 53
TRANIER ET AL.: "Commercial Biological Control Agents Targeted Against Plant-Parasitic Root-knot Nematodes", BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, vol. 57, no. 6, 2014, pages 831 - 841, XP055747646 *

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CN112410268A (zh) * 2020-12-09 2021-02-26 甘肃省农业科学院植物保护研究所 一株副地衣芽孢杆菌及其应用
CN112410268B (zh) * 2020-12-09 2021-11-05 甘肃省农业科学院植物保护研究所 一株副地衣芽孢杆菌及其应用
WO2022154653A1 (es) * 2021-01-14 2022-07-21 Becerra Carranza Luis Rodrigo Mezcla, formulación plaguicida y método, para controlar plagas de semillas y granos vegetales almacenados
WO2022191691A1 (es) * 2021-03-12 2022-09-15 Becerra Carranza Luis Rodrigo CEPA AISLADA DE Trichoderma Harzianum, MEZCLA Y MÉTODO, PARA PROMOVER LA GERMINACIÓN DE SEMILLAS VEGETALES
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CN114304188B (zh) * 2021-12-15 2023-02-28 宁夏大学 一种蜡蚧轮枝菌可分散油悬浮剂及其制备方法与应用
WO2023219483A1 (es) * 2022-05-09 2023-11-16 Becerra Carranza Luis Rodrigo Mezcla probiótica, biofungicida y método, para el control de hongos micotoxigénicos
CN117384805A (zh) * 2023-12-13 2024-01-12 中国科学院昆明植物研究所 一株抑制羊肚菌子囊果白霉病的环状尼氏芽孢杆菌及其应用
CN117384805B (zh) * 2023-12-13 2024-03-01 中国科学院昆明植物研究所 一株抑制羊肚菌子囊果白霉病的环状尼氏芽孢杆菌及其应用

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