WO2020143041A1 - Cell detection method - Google Patents

Cell detection method Download PDF

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WO2020143041A1
WO2020143041A1 PCT/CN2019/071415 CN2019071415W WO2020143041A1 WO 2020143041 A1 WO2020143041 A1 WO 2020143041A1 CN 2019071415 W CN2019071415 W CN 2019071415W WO 2020143041 A1 WO2020143041 A1 WO 2020143041A1
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content
cell
dna ploidy
sample
dna
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周双念
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深圳市双科生物科技有限公司
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Priority to PCT/CN2019/071415 priority Critical patent/WO2020143041A1/en
Publication of WO2020143041A1 publication Critical patent/WO2020143041A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • the invention relates to the field of cell detection, in particular to a cell detection method.
  • the object of the present invention is to provide a cell detection method to solve the problem of inaccurate detection results of the existing cell detection method.
  • a cell detection method including:
  • Detecting the expression state of L1 in the host cell of the L1 sample detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy
  • the performance of the cells in the sample is determined according to the content, and the performance of the cells includes a normal state, a suspicious state, and an abnormal state.
  • detecting the expression state of L1 in the L1 sample in the host cell detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy and content to judge the performance of the cells in the sample include:
  • detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, and judging the cell performance according to the DNA ploidy and content include:
  • detecting the expression state of L1 in the L1 sample in the host cell includes:
  • the expression state of L1 of the L1 sample in the host cell is positive; if there is no L1 antigen expression in cells in multiple fields of view, then the L1 The expression status of L1 in the host cell is negative.
  • detecting the DNA ploidy and content in the DNA ploidy and content analysis sample includes:
  • the two sub-samples were stained separately to form L1 samples and DNA ploidy and content analysis samples.
  • the beneficial effect of the present invention lies in: by detecting the L1 expression state and DNA ploidy and content of the sample in the host cell, according to the L1 expression state and DNA ploidy and content to determine whether the cell is abnormal, so as to be accurate Test cell performance.
  • FIG. 1 is a flowchart of a cell detection method provided by an embodiment of the present invention.
  • FIG. 2 is a flowchart of determining cell performance provided by an embodiment of the present invention.
  • the cell detection method provided by the embodiment of the present invention includes:
  • Step S101 Extract a sample.
  • the sample may be exfoliated cervical epithelial cells.
  • Step S102 Use the sample to make an L1 sample and a DNA ploidy and content analysis sample, respectively.
  • the L1 sample and the DNA ploidy and content analysis sample are two sub-samples extracted from the same sample. After the sub-samples are extracted, the two sub-samples are stained separately to form the L1 sample and the DNA ploidy and content analysis sample.
  • the A1 staining or fluorescent staining method was used for L1 samples
  • the DNA-Feulgen staining method was used for DNA ploidy and content analysis samples.
  • Step S103 detecting the expression state of L1 in the L1 sample in the host cell, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy and content determine the performance of the cells in the sample.
  • the performance of the cells includes normal state, suspicious state and abnormal state. Combined with the expression state of L1 and DNA ploidy and content to comprehensively judge whether the cell is abnormal, the accuracy rate is high.
  • this step includes:
  • Step S201 Detect the expression state of L1 in the host cell in the L1 sample.
  • this step includes: scanning multiple fields of view of the microscope. If the L1 antigen of at least one cell is expressed, the expression state of L1 of the L1 sample in the host cell is positive; if there are no cells in multiple fields of view When the L1 antigen is expressed, the expression state of L1 in the L1 sample in the host cell is negative.
  • Step S202 If the expression state of L1 in the host cell is positive, the cell is normal and the detection is stopped.
  • Step S203 If the expression state of L1 in the host cell is negative, detect the DNA ploidy and content in the DNA ploidy and content analysis sample, and judge the cell performance according to the DNA ploidy and content.
  • DNA ploidy and content analysis samples scan the sample cells, determine the cell DNA ploidy and content by cell morphology, label the DNA ploidy and content of each cell, and check the DNA ploidy and content of all cells. The content is counted, the DNA ploidy and content of each cell are calculated, the DNA ploidy and the cells whose content is significantly greater than the threshold are marked, and the DNA ploidy and content of the sample are calculated according to the statistical abnormal content peak.
  • Step S204 If the expression state of L1 in the host cell is negative, the DNA ploidy> 4.5, and the DNA content> 7.18 pg, the cell is abnormal and the chromosome is missing. Specifically, L1 protein expression disappeared, and DNA ploidy and content were abnormal.
  • Step S205 If the expression state of L1 in the host cell is negative, 2.2 ⁇ DNA ploidy ⁇ 4.5, DNA content>7.18pg, the cell is in a suspicious state, and the nuclear chromosome deletion is not obvious. Specifically, the L1 protein is free or integrated outside the cell nucleus, and the DNA ploidy is abnormal. Depending on the abnormal state of the sample, different intervention methods can be implemented on the cell.
  • Step S206 If the expression state of L1 in the host cell is negative, the DNA ploidy ⁇ 2.2, and the DNA content ⁇ 7.18 pg, the cell is normal.
  • the cell detection method provided by the invention can detect whether the cell is abnormal according to the L1 expression state and the DNA ploidy and content in the host cell by detecting the L1 expression state and DNA ploidy and content of the sample, so that the cell performance can be accurately detected.

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Abstract

A cell detection method, comprising: extracting a sample (S101); respectively preparing an L1 sample and a DNA ploidy and content analysis sample by using the sample (S102); detecting an expression state of L1 in a host cell in the L1 sample, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, and determining the performance of a cell in the sample according to the expression state of the L1 in the host cell and the DNA ploidy and content, wherein the performance of the cell comprises a normal state, a suspicious state, and an abnormal state (S103). According to the cell detection method, whether the cell is abnormal is determined according to the L1 expression state and the DNA ploidy and content, so that the abnormal state of the cells can be accurately detected.

Description

一种细胞检测方法A cell detection method 技术领域Technical field
本发明涉及细胞检测领域，尤其涉及一种细胞检测方法。The invention relates to the field of cell detection, in particular to a cell detection method.
背景技术Background technique
目前的细胞检测方法一般是通过检测样本的DNA倍体来判断细胞是否异常，由于受其他不确定因素影响较多，导致判断结果不够准确。目前也有通过单一检查L1表达来判断细胞是否异常的判断方法，当检测出L1表达为阴性时无法判断细胞为异常还是正常，从而难以判断L1表达为阴性时的意义，在L1表达状态为阴性时，判断结果也不准确。Current cell detection methods generally determine whether the cell is abnormal by detecting the DNA ploidy of the sample. Due to the influence of other uncertain factors, the judgment result is not accurate enough. At present, there is also a method for judging whether a cell is abnormal by single-checking L1 expression. When L1 expression is detected as negative, it is impossible to determine whether the cell is abnormal or normal, so it is difficult to determine the significance of L1 expression when negative. , The judgment result is also inaccurate.
发明内容Summary of the invention
为了克服现有技术的不足，本发明的目的在于提供一种细胞检测方法，以解决现有细胞检测方法检测结果不准确的问题。In order to overcome the shortcomings of the prior art, the object of the present invention is to provide a cell detection method to solve the problem of inaccurate detection results of the existing cell detection method.
本发明的目的采用如下技术方案实现：The purpose of the present invention is achieved by the following technical solutions:
一种细胞检测方法，包括：A cell detection method, including:
提取样本；Take samples
用所述样本分别制作L1样本和DNA倍体与含量分析样本；Use the samples to make L1 samples and DNA ploidy and content analysis samples;
检测所述L1样本中L1在宿主细胞中的表达状态，检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据所述L1在宿主细胞中的表达状态和所述DNA倍体与含量判断所述样本中的细胞的性能，所述细胞的性能包括正常状态、可疑状态和异常状态。Detecting the expression state of L1 in the host cell of the L1 sample, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy The performance of the cells in the sample is determined according to the content, and the performance of the cells includes a normal state, a suspicious state, and an abnormal state.
进一步地，检测所述L1样本中L1在宿主细胞中的表达状态，检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据所述L1在宿主细胞中的表达 状态和所述DNA倍体与含量判断所述样本中的细胞的性能包括：Further, detecting the expression state of L1 in the L1 sample in the host cell, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy and content to judge the performance of the cells in the sample include:
检测所述L1样本中L1在宿主细胞中的表达状态；Detecting the expression state of L1 in the host cell in the L1 sample;
若L1在宿主细胞中的表达状态呈阳性，则细胞为正常状态，停止检测；If the expression state of L1 in the host cell is positive, the cell is in a normal state and the detection is stopped;
若L1在宿主细胞中的表达状态呈阴性，则检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据DNA倍体与含量判断细胞的性能。If the expression state of L1 in the host cell is negative, then detect the DNA ploidy and content in the DNA ploidy and content analysis sample, and judge the cell performance according to the DNA ploidy and content.
进一步地，若L1在宿主细胞中的表达状态呈阴性，则检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据DNA倍体与含量判断细胞的性能包括：Further, if the expression state of L1 in the host cell is negative, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, and judging the cell performance according to the DNA ploidy and content include:
若L1在宿主细胞中的表达状态呈阴性，DNA倍体>4.5，DNA含量>7.18pg，则细胞为异常状态，染色体缺失；If the expression state of L1 in the host cell is negative, the DNA ploidy> 4.5, and the DNA content> 7.18 pg, the cell is abnormal and the chromosome is missing;
若L1在宿主细胞中的表达状态呈阴性，2.2<DNA倍体≤4.5，DNA含量>7.18pg，则细胞为可疑状态，细胞核染色体缺失不明显；If the expression state of L1 in the host cell is negative, 2.2 <DNA ploidy ≤ 4.5, DNA content> 7.18 pg, the cell is in a suspicious state, and the nuclear chromosome deletion is not obvious;
若L1在宿主细胞中的表达状态呈阴性，DNA倍体≤2.2，DNA含量≤7.18pg则细胞为正常状态。If the expression state of L1 in the host cell is negative, DNA ploidy ≤2.2, DNA content ≤7.18pg, the cell is normal.
进一步地，检测所述L1样本中L1在宿主细胞中的表达状态包括：Further, detecting the expression state of L1 in the L1 sample in the host cell includes:
扫描显微镜的多个视野，若至少一个细胞的L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阳性；若多个视野中的细胞都无L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阴性。In multiple fields of view of the scanning microscope, if the L1 antigen of at least one cell is expressed, the expression state of L1 of the L1 sample in the host cell is positive; if there is no L1 antigen expression in cells in multiple fields of view, then the L1 The expression status of L1 in the host cell is negative.
进一步地，检测所述DNA倍体与含量分析样本中的DNA倍体与含量包括：Further, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample includes:
对每个细胞的DNA倍体与含量进行标记，并对所有细胞的DNA倍体与含量进行统计，计算每个细胞的DNA倍体与含量，标记出DNA倍体与含量明显大于阈值的细胞。Mark the DNA ploidy and content of each cell, and count the DNA ploidy and content of all cells, calculate the DNA ploidy and content of each cell, and mark the cells with DNA ploidy and content significantly greater than the threshold.
进一步地，用所述样本分别制作L1样本和DNA倍体与含量分析样本包括：Further, using the samples to prepare L1 samples and DNA ploidy and content analysis samples respectively includes:
从同一份所述样本中提取出两个子样本；Extract two sub-samples from the same sample;
对两个所述子样本分别进行染色，形成L1样本和DNA倍体与含量分析样本。The two sub-samples were stained separately to form L1 samples and DNA ploidy and content analysis samples.
相比现有技术，本发明的有益效果在于：通过检测样本的L1在宿主细胞中的表达状态和DNA倍体与含量，根据L1表达状态和DNA倍体与含量判断细胞是否异常，从而可以准确检测细胞的性能。Compared with the prior art, the beneficial effect of the present invention lies in: by detecting the L1 expression state and DNA ploidy and content of the sample in the host cell, according to the L1 expression state and DNA ploidy and content to determine whether the cell is abnormal, so as to be accurate Test cell performance.
附图说明BRIEF DESCRIPTION
图1为本发明实施例提供的细胞检测方法流程图；1 is a flowchart of a cell detection method provided by an embodiment of the present invention;
图2为本发明实施例提供的判断细胞的性能的流程图。FIG. 2 is a flowchart of determining cell performance provided by an embodiment of the present invention.
具体实施方式detailed description
下面，结合附图以及具体实施方式，对本发明做进一步描述，需要说明的是，在不相冲突的前提下，以下描述的各实施例之间或各技术特征之间可以任意组合形成新的实施例。In the following, the present invention will be further described in conjunction with the drawings and specific implementations. It should be noted that, without conflict, the various embodiments described below or the technical features can be arbitrarily combined to form new embodiments. .
如图1所示，本发明实施例提供的细胞检测方法，包括：As shown in FIG. 1, the cell detection method provided by the embodiment of the present invention includes:
步骤S101：提取样本。Step S101: Extract a sample.
较佳的，样本可以为宫颈上皮脱落细胞。Preferably, the sample may be exfoliated cervical epithelial cells.
步骤S102：用所述样本分别制作L1样本和DNA倍体与含量分析样本。Step S102: Use the sample to make an L1 sample and a DNA ploidy and content analysis sample, respectively.
较佳的，L1样本和DNA倍体与含量分析样本是从同一份样本中提取出两个子样本，提取出子样本后，对两个子样本分别进行染色，形成L1样本和DNA倍体与含量分析样本。其中，对L1样本采用AEC染色或荧光染色方法，DNA倍体与含量分析样本采用DNA-Feulgen染色方法。Preferably, the L1 sample and the DNA ploidy and content analysis sample are two sub-samples extracted from the same sample. After the sub-samples are extracted, the two sub-samples are stained separately to form the L1 sample and the DNA ploidy and content analysis sample. Among them, the A1 staining or fluorescent staining method was used for L1 samples, and the DNA-Feulgen staining method was used for DNA ploidy and content analysis samples.
步骤S103：检测所述L1样本中L1在宿主细胞中的表达状态，检测所述 DNA倍体与含量分析样本中的DNA倍体与含量，根据所述L1在宿主细胞中的表达状态和所述DNA倍体与含量判断所述样本中的细胞的性能，所述细胞的性能包括正常状态、可疑状态和异常状态。结合L1表达状态和DNA倍体与含量综合判断细胞是否异常，准确率较高。Step S103: detecting the expression state of L1 in the L1 sample in the host cell, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy and content determine the performance of the cells in the sample. The performance of the cells includes normal state, suspicious state and abnormal state. Combined with the expression state of L1 and DNA ploidy and content to comprehensively judge whether the cell is abnormal, the accuracy rate is high.
较佳的，该步骤包括：Preferably, this step includes:
步骤S201：检测所述L1样本中L1在宿主细胞中的表达状态。Step S201: Detect the expression state of L1 in the host cell in the L1 sample.
较佳的，该步骤包括：扫描显微镜的多个视野，若至少一个细胞的L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阳性；若多个视野中的细胞都无L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阴性。Preferably, this step includes: scanning multiple fields of view of the microscope. If the L1 antigen of at least one cell is expressed, the expression state of L1 of the L1 sample in the host cell is positive; if there are no cells in multiple fields of view When the L1 antigen is expressed, the expression state of L1 in the L1 sample in the host cell is negative.
步骤S202：若L1在宿主细胞中的表达状态呈阳性，则细胞正常，停止检测。Step S202: If the expression state of L1 in the host cell is positive, the cell is normal and the detection is stopped.
步骤S203：若L1在宿主细胞中的表达状态呈阴性，则检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据DNA倍体与含量判断细胞的性能。Step S203: If the expression state of L1 in the host cell is negative, detect the DNA ploidy and content in the DNA ploidy and content analysis sample, and judge the cell performance according to the DNA ploidy and content.
较佳的，对于DNA倍体与含量分析样本，扫描样本细胞，通过细胞形态判断细胞DNA倍体与含量，对每个细胞的DNA倍体与含量进行标记，并对所有细胞的DNA倍体与含量进行统计，计算每个细胞的DNA倍体与含量，标记出DNA倍体与含量明显大于阈值的细胞，根据统计的异常含量峰值计算出样本的DNA倍体与含量。Preferably, for DNA ploidy and content analysis samples, scan the sample cells, determine the cell DNA ploidy and content by cell morphology, label the DNA ploidy and content of each cell, and check the DNA ploidy and content of all cells. The content is counted, the DNA ploidy and content of each cell are calculated, the DNA ploidy and the cells whose content is significantly greater than the threshold are marked, and the DNA ploidy and content of the sample are calculated according to the statistical abnormal content peak.
步骤S204：若L1在宿主细胞中的表达状态呈阴性，DNA倍体>4.5，DNA含量>7.18pg，则细胞异常，染色体缺失。具体为L1蛋白表达消失，DNA倍体与含量异常。Step S204: If the expression state of L1 in the host cell is negative, the DNA ploidy> 4.5, and the DNA content> 7.18 pg, the cell is abnormal and the chromosome is missing. Specifically, L1 protein expression disappeared, and DNA ploidy and content were abnormal.
步骤S205：若L1在宿主细胞中的表达状态呈阴性，2.2<DNA倍体≤4.5，DNA含量>7.18pg，则细胞为可疑状态，细胞核染色体缺失不明显。具体为L1蛋白游离或整合于细胞核外，DNA倍体较为异常，根据样本不同的异常状态， 可以对细胞实施不同的干预方法。Step S205: If the expression state of L1 in the host cell is negative, 2.2<DNA ploidy≤4.5, DNA content>7.18pg, the cell is in a suspicious state, and the nuclear chromosome deletion is not obvious. Specifically, the L1 protein is free or integrated outside the cell nucleus, and the DNA ploidy is abnormal. Depending on the abnormal state of the sample, different intervention methods can be implemented on the cell.
步骤S206：若L1在宿主细胞中的表达状态呈阴性，DNA倍体≤2.2，DNA含量≤7.18pg，则细胞正常。Step S206: If the expression state of L1 in the host cell is negative, the DNA ploidy ≤ 2.2, and the DNA content ≤ 7.18 pg, the cell is normal.
本发明提供的细胞检测方法，通过检测样本的L1在宿主细胞中的表达状态和DNA倍体与含量，根据L1表达状态和DNA倍体与含量判断细胞是否异常，从而可以准确检测细胞的性能。The cell detection method provided by the invention can detect whether the cell is abnormal according to the L1 expression state and the DNA ploidy and content in the host cell by detecting the L1 expression state and DNA ploidy and content of the sample, so that the cell performance can be accurately detected.
上述实施方式仅为本发明的优选实施方式，不能以此来限定本发明保护的范围，本领域的技术人员在本发明的基础上所做的任何非实质性的变化及替换均属于本发明所要求保护的范围。The above-mentioned embodiments are only preferred embodiments of the present invention, and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and replacements made by those skilled in the art on the basis of the present invention belong to the present invention. The scope of protection.

Claims (6)

  1. 一种细胞检测方法，其特征在于，包括：A cell detection method, characterized in that it includes:
    提取样本；Take samples
    用所述样本分别制作L1样本和DNA倍体与含量分析样本；Use the samples to make L1 samples and DNA ploidy and content analysis samples;
    检测所述L1样本中L1在宿主细胞中的表达状态，检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据所述L1在宿主细胞中的表达状态和所述DNA倍体与含量判断所述样本中的细胞的性能，所述细胞的性能包括正常状态、可疑状态和异常状态。Detecting the expression state of L1 in the host cell of the L1 sample, detecting the DNA ploidy and content in the DNA ploidy and content analysis sample, according to the expression state of the L1 in the host cell and the DNA ploidy The performance of the cells in the sample is determined according to the content, and the performance of the cells includes a normal state, a suspicious state, and an abnormal state.
  2. 根据权利要求1所述的细胞检测方法，其特征在于，检测所述L1样本中L1在宿主细胞中的表达状态，检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据所述L1在宿主细胞中的表达状态和所述DNA倍体与含量判断所述样本中的细胞的性能包括：The cell detection method according to claim 1, characterized in that the expression status of L1 in the host cell in the L1 sample is detected, the DNA ploidy and content in the DNA ploidy and content analysis sample are detected, according to the The expression status of the L1 in the host cell and the DNA ploidy and content to judge the performance of the cells in the sample include:
    检测所述L1样本中L1在宿主细胞中的表达状态；Detecting the expression state of L1 in the host cell in the L1 sample;
    若L1在宿主细胞中的表达状态呈阳性，则细胞为正常状态，停止检测；If the expression state of L1 in the host cell is positive, the cell is in a normal state and the detection is stopped;
    若L1在宿主细胞中的表达状态呈阴性，则检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据DNA倍体与含量判断细胞的性能。If the expression state of L1 in the host cell is negative, the DNA ploidy and content in the DNA ploidy and content analysis sample are detected, and the cell performance is judged according to the DNA ploidy and content.
  3. 根据权利要求2所述的细胞检测方法，其特征在于，若L1在宿主细胞中的表达状态呈阴性，则检测所述DNA倍体与含量分析样本中的DNA倍体与含量，根据DNA倍体与含量判断细胞的性能包括：The cell detection method according to claim 2, wherein if the expression state of L1 in the host cell is negative, the DNA ploidy and content in the DNA ploidy and content analysis sample are detected, according to the DNA ploidy The performance of cells judged by content includes:
    若L1在宿主细胞中的表达状态呈阴性，DNA倍体>4.5，DNA含量>7.18pg，则细胞为异常状态，染色体缺失；If the expression state of L1 in the host cell is negative, the DNA ploidy> 4.5, and the DNA content> 7.18 pg, the cell is abnormal and the chromosome is missing;
    若L1在宿主细胞中的表达状态呈阴性，2.2<DNA倍体≤4.5，DNA含量>7.18pg，则细胞为可疑状态，细胞核染色体缺失不明显；If the expression state of L1 in the host cell is negative, 2.2 <DNA ploidy ≤ 4.5, DNA content> 7.18 pg, the cell is in a suspicious state, and the nuclear chromosome deletion is not obvious;
    若L1在宿主细胞中的表达状态呈阴性，DNA倍体≤2.2，DNA含量≤7.18pg，则细胞为正常状态。If the expression state of L1 in the host cell is negative, DNA ploidy ≤2.2, DNA content ≤7.18pg, the cell is in a normal state.
  4. 根据权利要求2所述的细胞检测方法，其特征在于，检测所述L1样本中L1在宿主细胞中的表达状态包括：The cell detection method according to claim 2, wherein detecting the expression state of L1 in the host cell in the L1 sample comprises:
    扫描显微镜的多个视野，若至少一个细胞的L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阳性；若多个视野中的细胞都无L1抗原表达，则所述L1样本的L1在宿主细胞中的表达状态为阴性。In multiple fields of view of the scanning microscope, if the L1 antigen of at least one cell is expressed, the expression state of L1 of the L1 sample in the host cell is positive; if there is no L1 antigen expression in cells in multiple fields of view, then the L1 The expression status of L1 in the host cell is negative.
  5. 根据权利要求1所述的细胞检测方法，其特征在于，检测所述DNA倍体与含量分析样本中的DNA倍体与含量包括：The cell detection method according to claim 1, wherein detecting the DNA ploidy and content in the DNA ploidy and content analysis sample comprises:
    对每个细胞的DNA倍体与含量进行标记，并对所有细胞的DNA倍体与含量进行统计，计算每个细胞的DNA倍体与含量，标记出DNA倍体与含量明显大于阈值的细胞。Mark the DNA ploidy and content of each cell, and count the DNA ploidy and content of all cells, calculate the DNA ploidy and content of each cell, and mark the cells with DNA ploidy and content significantly greater than the threshold.
  6. 根据权利要求1所述的细胞检测方法，其特征在于，用所述样本分别制作L1样本和DNA倍体与含量分析样本包括：The cell detection method according to claim 1, wherein the preparation of an L1 sample and a DNA ploidy and content analysis sample using the sample respectively comprises:
    从同一份所述样本中提取出两个子样本；Extract two sub-samples from the same sample;
    对两个所述子样本分别进行染色，形成L1样本和DNA倍体与含量分析样本。The two sub-samples were stained separately to form L1 samples and DNA ploidy and content analysis samples.
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