WO2020117170A1 - Glucose biosensor based on polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer - Google Patents
Glucose biosensor based on polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer Download PDFInfo
- Publication number
- WO2020117170A1 WO2020117170A1 PCT/TR2019/051023 TR2019051023W WO2020117170A1 WO 2020117170 A1 WO2020117170 A1 WO 2020117170A1 TR 2019051023 W TR2019051023 W TR 2019051023W WO 2020117170 A1 WO2020117170 A1 WO 2020117170A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- polyphosphonoundecyl
- polyvinylimidazole
- glucose
- group
- electrode
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
- A61B5/1486—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using enzyme electrodes, e.g. with immobilised oxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2505/00—Evaluating, monitoring or diagnosing in the context of a particular type of medical care
- A61B2505/07—Home care
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
- A61B2562/12—Manufacturing methods specially adapted for producing sensors for in-vivo measurements
Definitions
- the invention is related to biosensors that can measure glucose from sweat.
- the invention is particularly related to a biosensor comprising a polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co- polyglycidyl methacrylate tetra block copolymer as an electron transmitter between the glucose oxidase and redox centre of the electrode for measuring glucose.
- Glucometers that perform electrochemical measurements formed of an electronic circuit and strips that measure glucose from blood are present in the market. Besides these, similar studies that have been published for detecting glucose from sweat are also available. For example in the study of Gao W. et ah, (1) glucose measurement from sweat where chitosan/carbon nanotube/glucose oxidase/ Au/Prussian blue is used, has been examined. In literature, glucose biosensor studies prepared with polymers such as PANI (2), ferrocene, chitosan are available. Nanoparticles such as carbon nanotubes and/or gold, as an electrical transmitter between the redox centre of glucose oxidase and the electrode are also used.
- Glucometers that measure glucose from blood, and that are sold commercially perform measurements from high concentrations such as 1-3 mM.
- a method of producing a glucose sensor by using ionic liquid electro coating nanometre material, which does not comprise an enzyme comprises the steps of, grinding a vitreous carbon electrode until the surface is cleaned by using a gamma-aluminium oxide powder.
- the precision of the sensors used to measure the glucose amount in the blood is low.
- These sensors are generally designed as disposable sensors.
- the stability of the enzyme electrode used in these sensors breakdown rapidly.
- biosensor which comprises polyphosphonoundecyl acrylate-co- polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer and which can thereby provide continuous measurement and higher precision was required.
- the invention aims to provide a biosensor where polyphosphonoundecyl acrylate- co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer is used as an electron transfer mediator between the glucose oxidase and redox centre of the electrode.
- Another aim of the invention is to carry out a biosensor which can provide continuous measurement and higher precision by means of its copolymer content.
- polyphosphonoundecyl acrylate-co-polyvinylimidazole-co- polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer was synthesized using 11-phosphonoundecyl acrylate, 1-vinylimidazole, vinylferrocene, glycidyl methacrylate monomers.
- the application of the polymer obtained by using 4 different monomer units as a glucose biosensor provides several advantages such as, improving the measurement of each monomer respectively (enabling measurement at very low detection limits (10 mM)), being able to measure several times with the same electrode, providing non-invasive glucose measurement and carrying out glucose measurement from sweat.
- the glucose oxidase enzyme polymeric electron transfer receiver for measuring glucose has been covalently bonded to epoxy groups in the polyphosphonoundecyl acrylate-co-polyvinylferrocene-co-polyglycidyl methacrylate copolymer.
- An azobisisobutyronitrile (AIBN) polymer starter and the polymer subject to the invention have been prepared at different ratios at a temperature of 70°C by means of the free radical copolymerization method.
- Tetra block copolymer acts as an electron transfer mediator between glucose oxidase and the redox centre of the electrode. It has been enabled for epoxy groups to easily bind to copolymers by means of this method.
- the (A) group is a polyphosphonoundecyl group and it comprises a phosphono group (PO3H2) and functional groups whose alkyl chain numbers can vary between 11-15 C.
- the (B) group is a polyvinylimidazole group, and it can comprise methyl groups at the imidazole 2, 3, 5 positions.
- the (C) group comprises the ferrocene group.
- the (D) group may comprise the derivatives of the glycidyl group.
- glucose measurement has been enabled at concentrations up to 10 mM.
- concentrations up to 10 mM For example, Koh A. et al. (3) have mentioned that they can go as below as 200 mM concentration for measuring glucose concentration in sweat.
- At least 55 measurements can be taken with a single electrode of the biosensor designed with the polymer subject to the invention and it costs 50% less than other glucose measurement devices.
- a non-invasive glucose measurement has been carried out from a sweat sample without requiring to take a blood sample with a needle or lancet.
- the enzyme chemically bonds to the surface of the electrode and the same electrode can be used at least 55 times for measuring, as opposed to the disposable options provided by competitors.
- the level of detection (LOD) has been reduced to 10mM and thereby precision has been increased.
- the polymer claimed as the invention can perform glucose measurement from sweat in a very short period of time (10 seconds). It can be used in the health sector particularly for diabetes patients, in order to produce a glucose biosensor which gives fast results which is practical and provides high precision. Moreover, by means of the high stability of the polymer subject to the invention, several measurements (e.g. 55) can be taken with a single electrode, and therefore the cost of the product shall be reduced.
- the disposable glucose measurement strips available in the market can be used only once.
- the enzymes used in these sensors can physically bind to the surface of the electrode, they allow a single measurement only.
- the polymer we have developed the enzyme is chemically bonded to the surface of the electrode and it provides continuous glucose measurement (55 measurements). Moreover, the sensor we have developed is high precision, thereby it performs glucose measurement for concentrations as low as IOmM.
- our product provides continuous, painless, measurement from sweat samples, and thereby reduces costs up to at least 66% .
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Emergency Medicine (AREA)
- Genetics & Genomics (AREA)
- Optics & Photonics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Abstract
The invention is related to a biosensor comprising a polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer as an electron transmitter between the glucose oxidase and redox centre of the electrode for measuring glucose from sweat.
Description
GLUCOSE BIOSENSOR BASED ON POLYPHOSPHONOUNDECYL ACRYLATE-CO-POLYVINYLIMIDAZOLE-CO- POLYVINYLFERROCENE-CO-POLY GLY CID YL METHACRYLATE
TETRA BLOCK COPOLYMER
Technical Field
The invention is related to biosensors that can measure glucose from sweat.
The invention is particularly related to a biosensor comprising a polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co- polyglycidyl methacrylate tetra block copolymer as an electron transmitter between the glucose oxidase and redox centre of the electrode for measuring glucose.
Prior Art
Glucometers that perform electrochemical measurements formed of an electronic circuit and strips that measure glucose from blood are present in the market. Besides these, similar studies that have been published for detecting glucose from sweat are also available. For example in the study of Gao W. et ah, (1) glucose measurement from sweat where chitosan/carbon nanotube/glucose oxidase/ Au/Prussian blue is used, has been examined. In literature, glucose biosensor studies prepared with polymers such as PANI (2), ferrocene, chitosan are available. Nanoparticles such as carbon nanotubes and/or gold, as an electrical transmitter between the redox centre of glucose oxidase and the electrode are also used.
Glucometers that measure glucose from blood, and that are sold commercially perform measurements from high concentrations such as 1-3 mM.
Successful immobilization has been carried out in several sensor studies of enzymes such as glucose oxidase, peroxidase. However, when an electron transfer mediator is absent, the electron transfer effect of the enzyme is very low. The precision of
the biosensor can be improved by adding an electron transfer mediator to the system during biosensor construction. The determination of glucose concentration and high precision measurement is crucially important in clinical diagnosis. Glucose oxidase linked ferrocene based modified electrodes (redox-active polymers) have been used from past to present, in order to determine glucose. However, problems such as low precision and rapid breaking down of the stability of the enzyme electrode, are faced in these studies. At the same time, as the enzyme physically binds to the electrode surface, the strips are disposable.
Besides this, various agents are also used such as gold nanoparticles and/or carbon nanotube, in order to increase precision. However, these agents necessitate high potential applications to be carried out on the electrode. The reduction of the applied potential by means of improving the communication between the nanomaterial and the electrode is significantly important for the electrode to be long-lasting.
In the Chinese patent document numbered CN104569116A of the prior art, a method of producing a glucose sensor by using ionic liquid electro coating nanometre material, which does not comprise an enzyme, is disclosed. This method comprises the steps of, grinding a vitreous carbon electrode until the surface is cleaned by using a gamma-aluminium oxide powder.
distributing graphene into perfluorosulfonate-polytetrafluoroethylene copolymer solution and preparing a black suspension liquid,
coating the vitreous carbon electrode with the black suspension liquid and preparing a graphene -perfluorosulfonate-polytetrafluoroethylene copolymer/vitreous carbon electrode,
adding palladium dichloride into 1 -ethyl-3 -methylimidazole tetrafluoroborate in order to prepare a precipitation liquid,
preparing a palladium nano-ion/graphene-chitosan/ vitreous carbon electrode to function as a study electrode by adding graphene-chitosan/ vitreous carbon electrode into the precipitation liquid,
- preparing a glucose sensor without an enzyme to be used in order to determine glucose by means of an electrochemical workstation by taking a saturated calomel electrode, and platinum wire as a reference electrode.
In the Russian patent document numbered RU2641966C2 of the prior art, an encapsulation of a glucose sensor, with a glucose oxidase enzyme that is present in a crosslinked hydrophilic copolymer which is in contact with the surface of the electrode, is disclosed. The structure of the hydrophilic copolymer is mentioned in the related document.
In the prior art, the precision of the sensors used to measure the glucose amount in the blood is low. These sensors are generally designed as disposable sensors. Moreover the stability of the enzyme electrode used in these sensors breakdown rapidly.
When the applications in the prior art are taken into consideration, it was realized that a biosensor which comprises polyphosphonoundecyl acrylate-co- polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer and which can thereby provide continuous measurement and higher precision was required.
Aims of the Invention
The invention aims to provide a biosensor where polyphosphonoundecyl acrylate- co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer is used as an electron transfer mediator between the glucose oxidase and redox centre of the electrode.
Another aim of the invention is to carry out a biosensor which can provide continuous measurement and higher precision by means of its copolymer content.
Detailed Description of the Invention
In the invention polyphosphonoundecyl acrylate-co-polyvinylimidazole-co- polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer was synthesized using 11-phosphonoundecyl acrylate, 1-vinylimidazole, vinylferrocene, glycidyl methacrylate monomers. The application of the polymer obtained by using 4 different monomer units as a glucose biosensor provides several advantages such as, improving the measurement of each monomer respectively (enabling measurement at very low detection limits (10 mM)), being able to measure several times with the same electrode, providing non-invasive glucose measurement and carrying out glucose measurement from sweat.
The synthesis scheme of the polymer is given below. (Scheme 1)
A
(Formula I)
In the invention, the glucose oxidase enzyme polymeric electron transfer receiver for measuring glucose has been covalently bonded to epoxy groups in the polyphosphonoundecyl acrylate-co-polyvinylferrocene-co-polyglycidyl methacrylate copolymer. An azobisisobutyronitrile (AIBN) polymer starter and the polymer subject to the invention have been prepared at different ratios at a temperature of 70°C by means of the free radical copolymerization method. Tetra block copolymer acts as an electron transfer mediator between glucose oxidase and the redox centre of the electrode. It has been enabled for epoxy groups to easily bind to copolymers by means of this method. Thereby the polymer that can be prepared in a single step, enables to obtain of a biosensor that can carry out rapid, practical and high precision glucose measurement.
In the copolymer of the invention, the (A) group is a polyphosphonoundecyl group and it comprises a phosphono group (PO3H2) and functional groups whose alkyl chain numbers can vary between 11-15 C. The (B) group is a polyvinylimidazole group, and it can comprise methyl groups at the imidazole 2, 3, 5 positions. The (C) group, comprises the ferrocene group. The (D) group may comprise the derivatives of the glycidyl group.
By means of the polyphosphonoundecyl acrylate-co-polyvinylimidazole-co- polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer used during biosensor construction, glucose measurement has been enabled at concentrations up to 10 mM. For example, Koh A. et al. (3) have mentioned that they can go as below as 200 mM concentration for measuring glucose concentration in sweat.
At least 55 measurements can be taken with a single electrode of the biosensor designed with the polymer subject to the invention and it costs 50% less than other glucose measurement devices. By means of the polymer subject to the invention, a non-invasive glucose measurement has been carried out from a sweat sample without requiring to take a blood sample with a needle or lancet.
By means of the synthesized polymer of the invention, the enzyme chemically bonds to the surface of the electrode and the same electrode can be used at least 55 times for measuring, as opposed to the disposable options provided by competitors.
Thanks to the polymer structure of the invention, the level of detection (LOD) has been reduced to 10mM and thereby precision has been increased.
The polymer claimed as the invention can perform glucose measurement from sweat in a very short period of time (10 seconds). It can be used in the health sector particularly for diabetes patients, in order to produce a glucose biosensor which gives fast results which is practical and provides high precision.
Moreover, by means of the high stability of the polymer subject to the invention, several measurements (e.g. 55) can be taken with a single electrode, and therefore the cost of the product shall be reduced. The disposable glucose measurement strips available in the market can be used only once. As the enzymes used in these sensors can physically bind to the surface of the electrode, they allow a single measurement only. By means of the polymer, we have developed the enzyme is chemically bonded to the surface of the electrode and it provides continuous glucose measurement (55 measurements). Moreover, the sensor we have developed is high precision, thereby it performs glucose measurement for concentrations as low as IOmM.
By means of the polymer we have developed, our product provides continuous, painless, measurement from sweat samples, and thereby reduces costs up to at least 66% .
REFERENCES
1. Gao, W. ei al,“Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis.’’Nature, 1529, 509-514, (2016)
2. Garjonyte, R., Malinauskas, A.,“Amperometric glucose biosensors based on Prussian Blue- and polyaniline-glucose oxidase modified electrodes.” Biosensors & Bioelectronics, 15, 445-451, (2000)
3. Koh, A.,“A soft, wearable microfluidic device for the capture, storage, and colorimetric sensing of sweat.” Science Translational Medicine , 8(366): 366ral65, (2016)
Claims
1. The invention is a biosensor comprising a polyphosphonoundecyl acrylate- co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl
methacrylate tetra block copolymer such as in Formula I, which is used for bonding the glucose oxidase enzyme to the surface of the electrode,
A7
(Formula I)
characterized by;
(A) being the polyphosphonoundecyl group,
(B) being the polyvinylimidazole group,
(C) being the ferrocene group,
(D) being the glycidyl group.
2. A biosensor according to claim 1, characterized in that, the polyphosphonoundecyl group (A) of the copolymer comprises a phosphono
group (PO3H2) and functional groups whose alkyl chain numbers can vary between 11-15 C.
3. A biosensor according to claim 2, characterized in that it comprises methyl groups at the imidazole 2, 3, 5 positions in the polyvinylimidazole group (B) of the copolymer.
4. A biosensor according to claim 3, characterized in that azobisisobutyronitrile (AIBN) is used as a polymer starter in order to obtain the copolymer.
5. A biosensor according to claim 4, characterized in that the enzyme is chemically bonded to the surface of the electrode by means of the copolymer.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TR2018/18902 | 2018-12-07 | ||
TR2018/18902A TR201818902A2 (en) | 2018-12-07 | 2018-12-07 | POLIFOSFONOUNDESIL ACRYLATE-CO-POLYVINYLIMIDAZOL-CO-POLYVINYLFERROSEN-CO-POLYGLICIDYL METHACRYLATE TETRA BLOCK COPOLYMER BASED GLUCOSE BIOSENSOR |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2020117170A1 true WO2020117170A1 (en) | 2020-06-11 |
Family
ID=70973759
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/TR2019/051023 WO2020117170A1 (en) | 2018-12-07 | 2019-12-03 | Glucose biosensor based on polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer |
Country Status (2)
Country | Link |
---|---|
TR (1) | TR201818902A2 (en) |
WO (1) | WO2020117170A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024103490A1 (en) * | 2022-11-16 | 2024-05-23 | 深圳可孚生物科技有限公司 | Preparation method for synthetase for continuous blood glucose monitoring |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8701282B2 (en) * | 1998-10-08 | 2014-04-22 | Abbott Diabetes Care Inc. | Method for manufacturing a biosensor |
-
2018
- 2018-12-07 TR TR2018/18902A patent/TR201818902A2/en unknown
-
2019
- 2019-12-03 WO PCT/TR2019/051023 patent/WO2020117170A1/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8701282B2 (en) * | 1998-10-08 | 2014-04-22 | Abbott Diabetes Care Inc. | Method for manufacturing a biosensor |
Non-Patent Citations (2)
Title |
---|
ASHLESHA BHIDE ET AL.: "CLASP (Continuous lifestyle awareness through sweat platform): A novel sensor for simultaneous detection of alcohol and glucose from passive perspired sweat", BIOSENSORS AND BIOELECTRONIC, vol. 117, 30 June 2018 (2018-06-30), pages 537 - 545, XP085435744, DOI: 10.1016/j.bios.2018.06.065 * |
H. LEE ET AL.: "Wearable/disposable sweat-based glucose monitoring device with multistage transdermal drug delivery module", SCIENCE ADVANCES, vol. 3, 2017, pages e1601314, XP055511563 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2024103490A1 (en) * | 2022-11-16 | 2024-05-23 | 深圳可孚生物科技有限公司 | Preparation method for synthetase for continuous blood glucose monitoring |
Also Published As
Publication number | Publication date |
---|---|
TR201818902A2 (en) | 2020-06-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Herrmann et al. | Hydrogels and their role in biosensing applications | |
Sehit et al. | Ultrasensitive nonenzymatic electrochemical glucose sensor based on gold nanoparticles and molecularly imprinted polymers | |
Aydemir et al. | Conducting polymer based electrochemical biosensors | |
Janegitz et al. | The application of graphene for in vitro and in vivo electrochemical biosensing | |
TWI503541B (en) | Biosensor and molecule-identifying member | |
Xu et al. | A wearable sensor based on multifunctional conductive hydrogel for simultaneous accurate pH and tyrosine monitoring in sweat | |
Yang et al. | In situ sampling and monitoring cell-free DNA of the Epstein–Barr virus from dermal interstitial fluid using wearable microneedle patches | |
JPWO2019146788A1 (en) | Protective membrane material for biosensor probes | |
CN105784796B (en) | A kind of sensitive determination method of the aptamer sensor based on gold/molybdenum disulfide/graphene nanocomposite material to lysozyme | |
JP2014057885A (en) | Sensor assembly for monitoring system of specimen using hydrogel, and monitoring method of specimen using sensor assembly | |
Barhoum et al. | Stimuli-bioresponsive hydrogels as new generation materials for implantable, wearable, and disposable biosensors for medical diagnostics: Principles, opportunities, and challenges | |
CN106556635B (en) | For sensing the biosensor and its manufacturing method of the analyte in sweat | |
US20120088997A1 (en) | Implantable biochip for managing trauma--induced hemorrhage | |
WO2011084607A1 (en) | Transdermal systems, devices, and methods for biological analysis | |
WO2020099560A1 (en) | Electrochemical sensor system comprising molecularly imprinted polymer for early warning of urinary tract infections | |
CN110522460A (en) | Multi-index detection sensor based on transdermal microneedle array and preparation method | |
CN111839532A (en) | Flexible epidermis electrochemistry biosensor based on conductive hydrogel | |
Wang et al. | A durable antifouling protein molecularly imprinted gel interface for human serum albumin detection and antibacterial application | |
WO2019187586A1 (en) | Sensor using phenazine derivative or high molecular weight redox polymer containing phenazine derivative | |
Liu et al. | Development of an amperometric biosensor on a toothbrush for glucose | |
WO2020117170A1 (en) | Glucose biosensor based on polyphosphonoundecyl acrylate-co-polyvinylimidazole-co-polyvinylferrocene-co-polyglycidyl methacrylate tetra block copolymer | |
Pan et al. | One-step synthesis of MXene-functionalized PEDOT: PSS conductive polymer hydrogels for wearable and noninvasive monitoring of sweat glucose | |
JP2015151436A (en) | Stimulation responsive gel material | |
Kotanen et al. | Characterization of a wireless potentiostat for integration with a novel implantable biotransducer | |
WO2019176339A1 (en) | Protective film material for biosensor probe |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 19892283 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
32PN | Ep: public notification in the ep bulletin as address of the adressee cannot be established |
Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 06.09.2021) |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 19892283 Country of ref document: EP Kind code of ref document: A1 |