WO2020050809A3 - Enrichment of samples inside the microchannels by using magnetic particles - Google Patents

Enrichment of samples inside the microchannels by using magnetic particles Download PDF

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Publication number
WO2020050809A3
WO2020050809A3 PCT/TR2019/050728 TR2019050728W WO2020050809A3 WO 2020050809 A3 WO2020050809 A3 WO 2020050809A3 TR 2019050728 W TR2019050728 W TR 2019050728W WO 2020050809 A3 WO2020050809 A3 WO 2020050809A3
Authority
WO
WIPO (PCT)
Prior art keywords
magnetic particles
biological
microchannels
enrichment
samples inside
Prior art date
Application number
PCT/TR2019/050728
Other languages
French (fr)
Other versions
WO2020050809A2 (en
Inventor
Utku HATIPOGLU
Barbaros CETIN
Gunes KIBAR
Original Assignee
Ihsan Dogramaci Bilkent Universitesi
Adana Bilim Ve Teknoloji Universitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ihsan Dogramaci Bilkent Universitesi, Adana Bilim Ve Teknoloji Universitesi filed Critical Ihsan Dogramaci Bilkent Universitesi
Priority to DE112019004459.1T priority Critical patent/DE112019004459T5/en
Priority to GB2104157.9A priority patent/GB2591406B/en
Publication of WO2020050809A2 publication Critical patent/WO2020050809A2/en
Publication of WO2020050809A3 publication Critical patent/WO2020050809A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates to purification method for selecting and isolating target substances from biological and/or chemical sample such as solutions containing DNA/RNA spirals, nanoscale biological and chemical structures such as exosomes, drug molecules, proteins, enzymes, cells and microbiological organisms, wherein it is particularly microfluidic based biological and/or chemical sample purification method by customized magnetic particles.
PCT/TR2019/050728 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles WO2020050809A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE112019004459.1T DE112019004459T5 (en) 2018-09-05 2019-09-05 ENRICHMENT OF SAMPLES BY MEANS OF MAGNETIC PARTICLES IN MICROCHANNELS
GB2104157.9A GB2591406B (en) 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2018/12647 2018-09-05
TR201812647 2018-09-05

Publications (2)

Publication Number Publication Date
WO2020050809A2 WO2020050809A2 (en) 2020-03-12
WO2020050809A3 true WO2020050809A3 (en) 2020-04-30

Family

ID=69721716

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2019/050728 WO2020050809A2 (en) 2018-09-05 2019-09-05 Enrichment of samples inside the microchannels by using magnetic particles

Country Status (3)

Country Link
DE (1) DE112019004459T5 (en)
GB (1) GB2591406B (en)
WO (1) WO2020050809A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022210704A1 (en) 2022-10-11 2024-04-11 Robert Bosch Gesellschaft mit beschränkter Haftung Cleaning cartridge for a microfluidic device, microfluidic device and method for cleaning the microfluidic device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8162149B1 (en) * 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
WO2013041983A1 (en) * 2011-09-19 2013-03-28 Centre National De La Recherche Scientifique Microfluidic system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4643822A (en) 1985-02-28 1987-02-17 The Secretary Of State For Trade And Industry In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method of separation of material from material mixtures
JP3115501B2 (en) 1994-06-15 2000-12-11 プレシジョン・システム・サイエンス株式会社 Method for controlling desorption of magnetic material using dispenser and various devices processed by this method
DE19500660B4 (en) 1994-12-10 2007-12-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for manipulating microscopic particles and their use
US5973138A (en) 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
JP3711988B2 (en) 2003-05-12 2005-11-02 株式会社日立製作所 Fine particle array analysis system, fine particle array kit, and chemical analysis method
AU2012220825B2 (en) * 2011-02-21 2015-12-17 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
US9709469B2 (en) * 2011-11-11 2017-07-18 The Regents Of The University Of California Valveless microfluidic device
CN103820304B (en) * 2014-02-25 2015-09-16 苏州天隆生物科技有限公司 Microfluid 3 D electromagnetic for nucleic acid purification excites evenly mixing device
US10962527B2 (en) * 2016-02-05 2021-03-30 The Broad Institute, Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8162149B1 (en) * 2009-01-21 2012-04-24 Sandia Corporation Particle sorter comprising a fluid displacer in a closed-loop fluid circuit
WO2013041983A1 (en) * 2011-09-19 2013-03-28 Centre National De La Recherche Scientifique Microfluidic system

Also Published As

Publication number Publication date
GB2591406B (en) 2023-03-01
GB2591406A (en) 2021-07-28
GB202104157D0 (en) 2021-05-05
DE112019004459T5 (en) 2021-07-15
WO2020050809A2 (en) 2020-03-12

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