WO2019231395A1 - Produit et appareil pour une manipulation améliorée de réacteurs pour traiter des échantillons biologiques - Google Patents

Produit et appareil pour une manipulation améliorée de réacteurs pour traiter des échantillons biologiques Download PDF

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Publication number
WO2019231395A1
WO2019231395A1 PCT/SG2018/050274 SG2018050274W WO2019231395A1 WO 2019231395 A1 WO2019231395 A1 WO 2019231395A1 SG 2018050274 W SG2018050274 W SG 2018050274W WO 2019231395 A1 WO2019231395 A1 WO 2019231395A1
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WO
WIPO (PCT)
Prior art keywords
reactor
guard
module
holder
biological sample
Prior art date
Application number
PCT/SG2018/050274
Other languages
English (en)
Inventor
Haiqing Gong
Original Assignee
Star Array Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Star Array Pte Ltd filed Critical Star Array Pte Ltd
Priority to PCT/SG2018/050274 priority Critical patent/WO2019231395A1/fr
Publication of WO2019231395A1 publication Critical patent/WO2019231395A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/085—Protection against injuring the user
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips

Definitions

  • the present invention relates to the field of biological sample processing and specifically processing of nucleic acids for analyses.
  • Bio samples are processed for various purposes like research, medical diagnosis and the kind.
  • the biological samples maybe in the form of nucleic acids, proteins, antibodies, antigens, body cells and so on.
  • PCR polymerase chain reaction
  • a large number of biological researchers use PCR and other forms of DNA amplifications in their work on nucleic acid analyses, due to its high sensitivity and specificity.
  • the PCR is conducted in a PCR thermal cycler where the nucleic acid with required reaction constituent undergoes thermal processing for DNA amplification.
  • the biological samples may also be reacted with biomarkers and without going through the amplification process by PCR, such as for immunoassays and cell based assays.
  • the biological sample containing nucleic acid are processed with nucleic acid extraction reagents to obtain nucleic acid in a purified or un-purified form, which are then loaded into reactors in form of tubes, glass capillaries or wellplates in a sample preparation module (SPM). Thereafter the reactors are subjected to thermal processing such as thermal cycling in a PCR module (PCRM).
  • SPM sample preparation module
  • PCM PCR module
  • NAEM nucleic acid extraction module
  • AM additional assay module
  • SM capping or sealing module
  • CM centrifuging module
  • CM centrifuging module
  • Another method of analyzing nucleic assay can be directly loading the biological sample without any step of nucleic acid extraction into the reactor, sealing and capping the reactor for PCR thermal cycling or isothermal amplification.
  • a biochip 31 consists of reactors 15 in the form of wells.
  • the biological sample 21 is dispensed into the reactors 15 and sealed by a cover or a sealing fluid 30.
  • the biochip 31 is then mounted onto a reactor holder for the thermal processing.
  • Fig. 1(b) shows another form of biochip 31.
  • the biological sample 21 is loaded at the inlet 313 which flows into the reactors 15 via the network of channels 315.
  • the outlet 314 helps to vent out the air while filling in the biological sample 21 in the reactors 15.
  • Fig 1(c) illustrates reactors 15, often called as PCR tubes or glass capillaries.
  • the reactors 15 are suspended from a reactor holder 14.
  • the biological sample 21 is loaded into the reactors 15 and then the reactors 15 are sealed.
  • the reactors 15 then proceed to the PCR module for a batch processing.
  • a centrifuging step is often required for loading the biological sample 21 into the bottom of the reactors 15.
  • Fig 1(d) illustrates a commercially available microarray type reactor 15.
  • the reactor 15 is in the form of a flat substrate that is spotted with reaction constituents (not shown) over which biological sample 21 is placed for a reaction to take place. The outcome of the reaction is subsequently analyzed.
  • the reactor 15 is commonly made of glass for light transmission from an illumination light source such as an LED (not shown) into the biological sample 21 inside the reactor 15 and from the biological sample 21 to a photodetector (not shown) for analyses of the biological sample 21.
  • an illumination light source such as an LED (not shown) into the biological sample 21 inside the reactor 15 and from the biological sample 21 to a photodetector (not shown) for analyses of the biological sample 21.
  • Such thin glass reactors 15 in a capillary form are low cost consumables but are delicate and fragile, requiring careful handling particularly in the SPM, as in the PCRM commonly the reactors are robotically cycled between the baths.
  • the glass capillaries are typically of the size 40mm in length, 0.1 to 1 mm in diameter and 0.01 -0.2mm in wall thickness.
  • the microarrays provide sample analyses in a large scale.
  • the commonly used glass reactors 15 which are thin slides get prone to breakage and the spots with the markers or the markers with the biological samples often get marred while handling.
  • the present invention provides an apparatus for improved handling of the delicate and disposable low-cost reactors like the thin walled glass PCR tubes or the biochips.
  • This invention provides a great positive impact on rapid biological analysis using glass capillary reactors.
  • continuous or perforated reactor guard for processing biological sample, the reactor guard being removably attachable to a reactor holder for at least partially surrounding reactor(s) when held by the reactor holder, in use the reactor(s) being for receiving biological sample.
  • the reactor guard helps to prevent physical contact by the users at the surrounded and exposed areas after the reactor(s) is/are held by the reactor holder. This feature safe guards the reactors from damage due to any physical impact including physical contact by the users while handling them or transferring them from one location to another or while loading into any of the modules in an apparatus.
  • the fragile reactor such as a glass capillary is prevented from getting broken, which results in failure of biological sample processing and analysis, and biological contamination, etc.
  • the reactor guard when attached to the reactor holder allows substantially no relative movement between the guard and the holder.
  • This feature helps to protect the reactors from undergoing any physical impact with the reactor guard during any movement during the transfer from one location to another or while loading into any of the modules in an apparatus or during any centrifugal step for loading the biological sample deeper into the reactors or to remove the air pocket under the biological sample.
  • the glass capillary may break upon being pressed against the reactor guard during centrifugation.
  • the guard spins outward and carries the holder and the reactors to spin outward as well.
  • the reactor guard comprises at least two rotation holders at two opposite exterior sides of the guard, the rotation holders being fixedly attached or attachable to the guard near the upper part of the guard and proximate the top opening of the reactor(s) in use.
  • This feature is advantageous during the step of centrifugation in the centrifuge module for loading the biological sample deeper inside the reactors when in the form of capillaries.
  • a rotor support in the centrifuge module allows the guard to rest on and swing the lower part of the guard under centrifugal force.
  • the reactors as held by the reactor holder can still remain guarded by the reactor guard for greater safety from handling.
  • the reactor guard comprises a continuous or discontinuous hoop that is attached or attachable around the guard, the hoop further comprising two rotation holders being fixedly attached or attachable to the hoop such that in use the rotation holders are positioned near the upper part of the guard and proximate the top opening of the reactor(s).
  • the rotation holders are supported by the hoop, during the centrifugation the pressure on the guard is lesser and better distributed over the surface area of the guard than when the rotation holders are fixed to the guard directly thereby creating localised pressure on the guard surface.
  • the guard material when without the hoop needs to be stronger, hence is likely to be more expensive than when the hoop is used.
  • the reactor guard further comprises: a robotic arm to pick and place an assembly of the reactor holder holding the reactor(s) and the attached guard between two user specified positions. This feature is useful when the assembly needs to be transferred between several assay stations for processing the biological sample.
  • an assembly of the reactor guard that is removably attached to the reactor holder holding at least one reactor.
  • the user handling is further reduced as the reactor guard is already attached to the reactor holder holding the reactor(s).
  • apparatus for preparation of nucleic acid, for amplification by thermal processing comprising: a reactor holder for holding reactor(s) in use; and a continuous or perforated reactor guard that is removably attachable to a reactor holder for at least partially surrounding reactor(s) when held by the reactor holder, in use the reactor(s) being for receiving biological sample.
  • a reactor holder for holding reactor(s) in use
  • a continuous or perforated reactor guard that is removably attachable to a reactor holder for at least partially surrounding reactor(s) when held by the reactor holder, in use the reactor(s) being for receiving biological sample.
  • the apparatus further comprises: a first module that further comprises: a first unit including a plurality of extraction chambers for receiving biological sample and reagent(s) in use; and a first transfer arm for transferring biological sample from the extraction chambers by a plurality of pipettes to a first user specified location.
  • a first module that further comprises: a first unit including a plurality of extraction chambers for receiving biological sample and reagent(s) in use; and a first transfer arm for transferring biological sample from the extraction chambers by a plurality of pipettes to a first user specified location.
  • the apparatus further comprises a second unit including a plurality of assay chambers for receiving fluid(s) in use; and a second transfer arm for transferring fluid(s) from the assay chambers by a plurality of pipettes to a second user specified location.
  • the first transfer arm may be the same as the second transfer arm and the first user specified location may be the same as the second user specified location such as the location of the reactors.
  • the apparatus further comprises a container for accommodating the reactor holder while holding the reactor(s), the guard, the first unit and the second unit, at pre-defined positions within the container.
  • the container advantageously holds the first unit, the second unit and the reactors as held by the reactor holder at close and specified locations so that the biological sample from the extraction chambers can be transferred in a batch by a row of pipettes to the assay chambers and then loaded into the reactors.
  • the biological sample from the extraction chambers can be transferred in a batch by a row of pipettes into the reactors and assay reagents from the assay chambers can be also be loaded in a batch into the reactors by a row of pipettes.
  • the apparatus further comprises at least one module from the group consisting of: a second module for capping the reactor(s), a third module for sealing the reactor(s), a fourth module for centrifuging the reactor(s) to load biological sample in use, and a fifth module for processing of biological sample in use.
  • a robotic arm may be provided to pick and place the reactor holder holding the reactor(s) with the attached guard between the first module and any of the second, the third, or the fourth modules.
  • the apparatus further comprises a robotic arm to pick and place an assembly of the reactor holder holding the reactor(s) and with the attached guard between two user specified positions. This feature helps to relocate the delicate reactors with the biological such as to other modules and sub-modules in the apparatus for further processing while reducing the risk of manual contact with the reactors.
  • the guard when attached to the reactor holder allows substantially no relative movement between the guard and the holder.
  • the advantage is same as described under the first aspect.
  • the apparatus further comprises: at least two rotation holders at two opposite exterior sides of the guard, the rotation holders being fixedly attached or attachable to the guard near the upper part of the guard and proximate the top opening of the reactor in use; and a centrifuge module for centrifuging the reactor(s) for loading biological sample in use, the centrifuge module comprising a rotor support that allows the guard to rest on and swing the lower part of the guard under centrifugal force.
  • a continuous or discontinuous hoop is provided that is attached or attachable around the reactor guard, the hoop further comprising two rotation holders being fixedly attached or attachable to the hoop such that in use the rotation holders are positioned near the upper part of the guard and proximate the top opening of the reactor(s); and a centrifuge module for centrifuging the reactor(s) is provided for loading biological sample in use, the centrifuge module comprising a rotor support that allows the guard to rest on and swing the lower part of the guard under centrifugal force.
  • FIGs. 1(a) and (b) present perspective views of commercially available reactor array in bio chip form.
  • Fig. 1(c) presents a cross-sectional elevation view of commercially available capillary type reactors as held by a reactor holder.
  • Fig. 1(d) presents a plan view of a commercially available microarray type reactor.
  • Figs. 2(a) and (c) present plan views of two embodiments of the invention.
  • Figs. 2(b) and (d) present cross-sectional elevation views along the two dashed horizontal cut-lines shown in Figs 2(a) and 2(c) respectively.
  • Fig. 3(a) presents a plan view of an embodiments of the invention.
  • Fig. 3(b) presents a cross-sectional elevation view along the dashed horizontal cut-line shown in Fig 3(a).
  • Fig. 4a provides a flow diagram according to an embodiment of the invention with the cross-sectional elevation view of the reactors held by the reactor holder as in Fig. 2a along the vertical and dashed cut-line.
  • Fig. 4b provides an alternate embodiment of Fig. 4a.
  • Fig. 5 provides perspective exterior and interior views of the assembly of the reactor guard, the reactor holder and the reactors, according to an embodiment of the invention.
  • Fig. 6(a) provides a perspective view of the assembly of the reactor guard, the reactor holder and the reactors, where the guard is provided with a hoop and rotation holders at two opposite exterior sides of the hoop, according to an embodiment of the invention.
  • Fig. 6(b) provides a perspective view of the assembly of the reactor guard, the reactor holder and the reactors, where the guard is provided with rotation holders at two opposite exterior sides of the guard according to an embodiment of the invention.
  • Fig. 7(a) presents an embodiment with an elevation cross-sectional view along the cutline A-A’ at Figs. 6(a) or 6(b) with the rotation holder and a rotor support in the centrifuge module when not under a centrifugal force.
  • Fig. 7(b) presents an elevation side view of Fig. 7(a).
  • Fig. 7(c) an elevation view of Fig. 7(b) when under a centrifugal force.
  • Figs. 2(a) presents plan view of an embodiment of the invention for a sample preparation module 35.
  • a reactor holder 14 holds several reactors 15 in the form of tubes.
  • the reactors 15 are physically guarded by the reactor guard 12.
  • the reactor guard 12 may be continuous or perforated for any desirable reason.
  • the reactor guard 12 is removably attachable to the reactor holder 14 for at least partially surrounding the reactors 15 to prevent physical contact by the users at the surrounded and exposed areas after the reactors 15 are held by the reactor holder 14.
  • the reactor guard 12 may surround the sides as well as the bottom side of the reactors 15.
  • a pick-up connector 13 connects the reactor guard 12 and the reactor holder 14 together so that both can be picked-up together by the pick-up connector 13 manually or by a robotic arm (not shown) and transferred to any other module for subsequent steps if any, before the thermal processing.
  • the sample preparation module 35 includes a container 7 and a first unit 8 for nucleic acid extraction. The nucleic acid extraction is conducted in the extraction chambers 9. Herein, the biological sample 21 containing nucleic acid is processed.
  • the container 7 accommodates the reactor holder 14 while holding the reactors 15, the guard 12 and the first unit 8.
  • a second unit 10 is accommodated in the container 7 for assay of the extracted nucleic acid from the first unit 8.
  • the assay is conducted in the assay chambers 11 where the biological sample 21 from the first unit 8 is formulated.
  • Fig. 2(b) shows the cross-sectional elevation view along the horizontal and dashed cut-line in Fig. 2(a).
  • the extraction tube 9 contains a first reaction constituent 19 for purification and the assay tube 11 contains a second reaction constituent 20.
  • the biological sample 21 is picked up such as by pipettes (not shown) from the first unit 8 and transferred to the second unit 10.
  • the biological sample 21 is picked up such as by pipettes (not shown) and transferred to the reactor 15 for further processing, such as thermal processing to attain nucleic acid amplification.
  • the biological sample 21 and the second reaction constituent 20 may be loaded at the top opening of the reactor 15.
  • the reactor 15 is subsequently centrifuged for the biological sample 21 with the reaction constituent 20 to be loaded at the bottom of the reactor 15.
  • the reaction constituent 20 and the biological sample 21 may be loaded at the bottom of the reactor 15 by a pipette.
  • the extraction chambers 9 and assay chambers 11 may be in the form of tubes as well.
  • Figs. 2(c) presents plan view of an embodiment of the invention where the reactor 15 is in the form of a flat surface and pre-spotted with biomarkers and/or PCR primers.
  • the biological sample 21 is placed on these pre-spots surface by pipette in the form of a microarray.
  • Fig. 2(d) shows the cross-sectional elevation view along the horizontal and dashed cut-line in Fig. 2(c).
  • the reactor guard 12 partially surrounds the reactor 15 as held by the reactor holder 14. Subsequently, the assembly of the reactor 15 with the reactor holder 14 and the reactor guard 12 may be manually or robotically picked and placed elsewhere from this sample preparation module 35.
  • FIGs. 3(a) presents plan view of an embodiment of the invention where the reactors 15 of Fig. 2(a) are replaced by a biochip 31, as described under Fig. 1 (b).
  • Fig. 3(b) shows the cross- sectional elevation view along the horizontal and dashed cut-line in Fig. 3(a).
  • the reactor guard 12 surrounds the biochip 31 on three sides and has raised sides for protecting the top side of the bio-chip 31 from accidental physical contact by the users.
  • Fig. 4a provides a flow diagram according to an embodiment of the invention where the reactor holder 14 holding the reactors 15 and guarded by the reactor guard 12 as in Figs. 2(a) and (b) is transferred manually or by a robotic arm (not shown) while holding by the pick-up connector 13 to a capping module 40 for capping the reactors 15 with a cap 3 each. Thereafter, the assembly of the reactor holder 14 holding the reactors 15 and guarded by the reactor guard 12 is transferred manually or by a robotic arm (not shown) while holding by the pick-up connector 13 to a centrifuge module 45 for centrifuging the reactors 15 to load the biological sample 21 to the bottom of the reactors 15.
  • the assembly of the reactor holder 14 holding the reactors 15 and guarded by the reactor guard 12 is transferred manually or by a robotic arm (not shown) while holding by the pick-up connector 13 to the assay module 50 for processing of the biological sample 21 or testing and analysis of the biological sample 21.
  • the processing may include a step of polymerase chain reaction (PCR).
  • PCR polymerase chain reaction
  • the pick-up connector 13 is manually or robotically operated to disconnect the reactor holder 14 holding the reactor 13 from the reactor guard 12 before further processing.
  • the grip of the pick-up connector 13 by the robotic arm may be by any suitable means in the art such as vacuum or electromagnetic or mechanical.
  • a sealing liquid (not shown) may be used.
  • the sealing liquid may be a curable type such as wax, hot melt, glue or non-curable type such as oil, silicone, glue, wax.
  • the reactor guard 12 may be already mounted in any of the sample preparation module 35, the capping module 40 or the centrifuge module 45.
  • the reactor guard 12 may also be used in an optical module (not shown) during the optical analysis after the thermal processing of the biological sample 21.
  • the protection of the reactor guard 12 may be provided in at least one of the modules.
  • the pick-up connector 13 may be located in any convenient position in the assembly of the reactor holder 14 holding the reactors 15 and the reactor guard 12 and may or may not also serve the purpose of connecting the reactor guard 12 and the reactor holder 14 together.
  • the assembly of the reactor holder 14 holding the reactors 15 and guarded by the reactor guard 12 may also be transferred to user specified locations manually or by a robotic arm (not shown) by holding the reactor guard 12 instead of the pick-up connector 13.
  • the pick-up connector 13 may be detachable from the assembly.
  • the reactor holder 14 may be detachable from the assembly and particularly from the reactor guard 12.
  • the lock 16 locks the reactor guard 12 with the reactor holder 14. This is required when the assembly of the reactor guard 12 and the reactor holder 14 holding the reactors 15 is picked-up and transferred between various locations by the pick-up connector 13 or the reactor holder 14.
  • the locking mechanism may be of any type in the art such as mechanical, electromechanical, magnetic, frictional and the kind. The lock 16 however may not be required when the assembly is picked-up and transferred between various locations by a manual or an automated grip on the reactor guard 12 onto which the reactor holder 14 holding the reactors 15 may rest.
  • the steps of capping and centrifuging in the capping module 40 and the centrifuging module 45 respectively as in Fig. 4a may be executed by physically transferring the assembly of the reactors 15, the holder 14 and the reactor guard 12 out of the sample preparation module 35 to the capping module 40 and the centrifuging module 45.
  • the steps of capping and centrifuging may be executed in the sample preparation module 35 itself and without physically transferring the assembly of the reactors 15, the holder 14 and the reactor guard 12 out of the sample preparation module 35.
  • the reactor guard 12 may be detached before the step of thermal processing or testing as applicable. The detachment may also be done in the centrifuge module 45 or in the capping module 40 as applicable.
  • the guard 12 when attached to the reactor holder 14 may preferably allow no relative movement between the guard 12 and the holder 14. This feature helps to protect the reactors 15 as held by the holder 14 from undergoing any physical impact with the guard 12 during any movement during the transfer from one location to another or while loading into any of the modules in the apparatus. This feature is particularly helpful during any centrifugal step in the centrifuging module 45 where the whole assembly of the guard 12, the holder 14 and the reactors 15 held by the holder 14 undergoes a change in the angular position. [0051] Some of those modules as shown in Fig. 4a may be located within a single apparatus and some other modules may be standalone devices outside the apparatus. Workflows other than the workflow shown in Fig. 4a may also be deployed while using the reactor guard 12.
  • Fig. 4b illustrates an alternate embodiment without the need for a centrifuge step.
  • a thin pipette tip or a needle is used to load the biological sample 21 such as with the PCR master mix, directly to the bottom of the reactors 15 in the form of glass capillaries.
  • Fig. 5 provides perspective exterior and interior views of the assembly of the reactor guard 12, the reactor holder 14 and the reactors 15 in the form of glass capillaries, according to an embodiment of the invention.
  • Fig. 6(a) provides a perspective view of the assembly of the reactor guard 12, the reactor holder 14 and the reactors 15, where the guard 12 is provided with a hoop 103 around the top portion.
  • the hoop 103 may be fixed to the guard 12 or detachable.
  • the hoop 103 is further provided with fixed rotation holders 101 at two opposite exterior sides.
  • the rotation holders 101 may be fixed to the hoop 103 or detachable.
  • Fig. 6(b) provides a perspective view of the assembly of the reactor guard 12, the reactor holder 14 and the reactors 15, where the guard 12 itself is provided with the rotation holders 101 at two opposite exterior sides of the guard 12, without the use of the hoop 103.
  • Fig. 7(a) presents an embodiment with an elevation cross-sectional view along the cutline A- A’ at Figs. 6(a) or 6(b) with the rotation holder 101 and a rotor support 102 in the centrifuging module 45 when not under a centrifugal force.
  • the fixed rotation holders 101 at two opposite exterior sides of the guard 12 allows the guard 12 to rest on the rotor support 102 in the centrifuging module 45, such that before centrifugation the opening of the reactor 15 is facing up to allow loading of the biological sample 21, followed by reactor sealing or capping operations from the top.
  • Fig. 7(b) presents an elevation side view of Fig. 7(a).
  • Fig. 7(c) shows an elevation view of Fig. 7(b) when under a centrifugal force.
  • the guard 12 rotates about the rotation holder 101 on the rotor support 102 to allow the bottom of the reactor 15 to move outward while loading the sample 21 deeper inside the reactor 15.
  • the rotation holder 101 is located near the open and upper part of the guard 12 in order to allow the reactor 15 to incline outward during centrifugation.
  • the opening of the reactor 15 faces upwards when centrifugation stops.
  • the biological sample may also refer to reaction mixture containing nucleic acid, antibody, antigen, protein, cells, and chemical.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Une protection de réacteur continue ou perforée (12) est prévue pour traiter l'échantillon biologique (21), la protection de réacteur (12) pouvant être fixée de manière amovible à un support de réacteur (14) pour au moins partiellement le réacteur (s) (15) lorsqu'il est maintenu par le support de réacteur (14), lors de l'utilisation, le réacteur(s) (15) étant destiné à recevoir un échantillon biologique (21). L'invention concerne également un ensemble de la protection de réacteur (12) fixé amovible au support de réacteur (14) contenant au moins un réacteur (15). L'invention concerne un appareil comprenant le support de réacteur (14) pour maintenir un réacteur(s) (15) en utilisation et la protection de réacteur continue ou perforée (12).
PCT/SG2018/050274 2018-06-01 2018-06-01 Produit et appareil pour une manipulation améliorée de réacteurs pour traiter des échantillons biologiques WO2019231395A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/SG2018/050274 WO2019231395A1 (fr) 2018-06-01 2018-06-01 Produit et appareil pour une manipulation améliorée de réacteurs pour traiter des échantillons biologiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2018/050274 WO2019231395A1 (fr) 2018-06-01 2018-06-01 Produit et appareil pour une manipulation améliorée de réacteurs pour traiter des échantillons biologiques

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WO2019231395A1 true WO2019231395A1 (fr) 2019-12-05

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5833057A (en) * 1997-04-28 1998-11-10 Char; Aka Loka Apparatus for packaging and shipping biological fluid samples collected in vials
US20050025673A1 (en) * 1997-11-14 2005-02-03 Gen-Probe Incorporated Substance transfer device
EP1767274A1 (fr) * 2005-09-26 2007-03-28 Qiagen GmbH Procédé et dispositif pour le traitement d'un fluide
US20090130745A1 (en) * 2007-07-13 2009-05-21 Handylab, Inc. Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples
US20140277695A1 (en) * 2013-03-15 2014-09-18 Shazi Iqbal Automatic tracking of a specimen holder moved from one specimen rack to another

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5833057A (en) * 1997-04-28 1998-11-10 Char; Aka Loka Apparatus for packaging and shipping biological fluid samples collected in vials
US20050025673A1 (en) * 1997-11-14 2005-02-03 Gen-Probe Incorporated Substance transfer device
EP1767274A1 (fr) * 2005-09-26 2007-03-28 Qiagen GmbH Procédé et dispositif pour le traitement d'un fluide
US20090130745A1 (en) * 2007-07-13 2009-05-21 Handylab, Inc. Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples
US20140277695A1 (en) * 2013-03-15 2014-09-18 Shazi Iqbal Automatic tracking of a specimen holder moved from one specimen rack to another

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