WO2019216533A1 - Method for measuring viruses and mold in air - Google Patents

Method for measuring viruses and mold in air Download PDF

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Publication number
WO2019216533A1
WO2019216533A1 PCT/KR2019/002061 KR2019002061W WO2019216533A1 WO 2019216533 A1 WO2019216533 A1 WO 2019216533A1 KR 2019002061 W KR2019002061 W KR 2019002061W WO 2019216533 A1 WO2019216533 A1 WO 2019216533A1
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virus
mold
air
collecting
reagent
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PCT/KR2019/002061
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French (fr)
Korean (ko)
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황정호
김형래
안상권
변정훈
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연세대학교 산학협력단
영남대학교 산학협력단
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Publication of WO2019216533A1 publication Critical patent/WO2019216533A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2273—Atmospheric sampling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Definitions

  • the present invention is a method for determining the presence or absence of viruses or mold in the air, by reacting the collection unit separated from the air collecting device with the reagent, the air to measure the presence or absence of viruses and mold in the air through the change of reagent It relates to a heavy virus and fungal measurement method.
  • viruses In addition to dust, fine dust and pollen, the air contains many kinds of viruses and fungi, and huge numbers of viruses move along the air every day. Viruses found in one region are genetically similar, even in very remote areas. This shows that viruses can move into the air with dust and travel thousands of kilometers, and the area is not always safe just because a virus is not present.
  • viruses and fungi don't cause major problems, but some viruses and fungi can cause fatal illnesses that can completely destroy an organism in a given area and should always be used with caution.
  • Korean Patent Publication No. 10-1269173 (Registration Date: 2013.05.23) relates to a method and apparatus for measuring bacteria and viruses using a thin film integrated electronic device. Using this integrated electronic device, the electrochemical change of the thin film due to the metabolic action of the bacteria and the enzyme reaction derived from the virus is measured to determine the presence and concentration of bacteria and viruses.
  • the present invention is to provide a method for detecting viruses and mold in the air at a low cost.
  • the present invention is to provide a method that can quickly detect the virus and mold in areas where virus and mold contamination is suspected.
  • the present invention for achieving this object is a method for measuring viruses and fungi in the air, comprising the steps of combining the collecting unit to the collecting device; Operating the collecting device for a predetermined time to collect at least one of viruses and fungi in the air in the collecting unit; Separating the collecting device and the collecting part; And selecting whether to detect a virus or mold.
  • the collection unit is put in a container provided with a reagent, the virus collected in the collection unit Dissolving with the reagents; Reacting the reagent in which the virus is dissolved in the kit for a predetermined time; Determining the presence or absence of a virus through a change in the kit; Measuring the virus through and selecting whether to detect the virus or the mold, and when the detection of the mold is selected, rubbing the collection unit to an agar plate on which mold can grow; Storing the agar plates in the incubator for a predetermined time; And taking out the aga plate in the incubator to measure the mold; The mold can be measured via.
  • the collecting unit is a cotton swab, the cotton ball portion of the cotton swab is located in the air flow path of the collecting device.
  • the collecting device collects air at a flow rate of 8-10 lpm (liter per minute).
  • the collecting unit in the step of dissolving the virus collected in the collecting unit with the reagent, is added to 0.5 ml of the reagent and rotated 10 times to dissolve the virus.
  • the step of reacting the reagent in which the virus is dissolved in the kit for a predetermined time in the step of reacting the reagent in which the virus is dissolved in the kit for a predetermined time, five drops of the reagent in which the virus is dissolved in the kit are dropped.
  • the kit and the reagent in which the virus is dissolved are reacted for 5 to 8 minutes.
  • the agar plate in the step of storing the agar plate in the incubator for a predetermined time, is stored in the incubator for 24 hours.
  • the step of coupling the collecting unit to the collecting device operating the collecting device for a predetermined time to collect the air virus in the collecting unit and separating the collecting device and the collecting unit;
  • the virus detection is selected, the collecting part is put in a container equipped with 0.5 ml of the reagent and rotated 10 times.
  • the collecting device collects air at a flow rate of 8-10 lpm (liter per minute).
  • the virus and mold measurement method in the air according to the present invention has the effect of detecting viruses and molds at low cost by using a general cotton swab, without having to provide an expensive dedicated kit.
  • the present invention can quickly detect the presence or absence of viruses and fungi by detecting viruses and fungi in the collected air for a short time.
  • FIG. 1 is a flow chart for the process of measuring the virus and fungi with the virus and mold collecting device according to the present invention
  • Figure 2 is a front view of the virus and mold collecting device according to the present invention.
  • FIG. 3 is a view using a virus and mold collecting device according to the present invention.
  • Figure 5 is a measure of the fungi collected by the virus and the mold collecting device according to the invention.
  • Figure 1 shows a flow chart for the process of measuring viruses and fungi with the virus and fungi collection device according to the present invention.
  • the collecting unit is a cotton swab 20
  • the cotton ball 22 of the cotton swab 20 is a virus and mold collecting device 10 (hereinafter, referred to as 'collecting device' ) In the air flow path 13.
  • the collecting device 10 In operation S20 of collecting at least one of air virus and mold in the collecting unit by operating the collecting device 10, the collecting device 10 is operated for a predetermined time so that viruses and mold contained in the air Allow collection to be collected.
  • step S40 of selecting whether to detect a virus or a mold the collection part is put into a container equipped with a reagent, and the virus collected in the collection part is dissolved in a reagent.
  • the step (S50) and the step of detecting the virus-dissolved reagent in the kit (S60) and the change of the kit through the change of the kit (S70) can be further proceeded.
  • a plurality of swabs 20 added to the reagent 32 are stirred to dissolve the virus. Rotate about 10 times to allow the virus collected on the swab 20 to react sufficiently with the reagent 32.
  • the virus-dissolved reagent 32 is buried in the detection kit 40 and reacted for a predetermined time. Waiting for about 5-8 minutes for the reagent 32 and the detection kit 40 to sufficiently react, preferably 5 minutes.
  • the step of determining the presence or absence of the virus through the change of the kit (S70) to see the change in the display window 44 of the detection kit 40 may be to determine the presence of the virus in the air.
  • the presence or absence of virus in the air can be easily checked. If a virus is detected, a precise analysis staff can be put in to quantify the virus in the air.
  • the step (S40) of selecting whether to detect the virus or mold the step of coupling the collecting unit to the collecting device (S10) and operating the collecting device for a predetermined time In order to collect the mold in the air to the collecting unit (S20) and separating the collecting device and the collecting unit (S30) is made in the same manner as the virus detection process. However, after selecting the detection of the mold in the step (S40) of selecting whether to detect the virus or the mold, the mold is occupied by a process different from the virus detection process.
  • the step of selecting whether to detect the virus or the mold (S40) the step of rubbing the collecting part on the agar plate that can grow mold (S51) and the aga plate Storage in the incubator for a time (S61) and take out the agar plate from the incubator to measure the mold (S71) can be further proceeded.
  • step (S51) to rub the collecting part on the agar plate that mold can grow mold spores buried in the cotton swab 20 is collected in the agar plate 50.
  • the step of storing the agar plate in the incubator for a predetermined time provides the optimum conditions for the growth of the fungal spores to allow sufficient growth in the incubator.
  • the number of molds grown in the agar plate 50 may be determined how much mold is contained in the air.
  • Figure 2 relates to a front view of the virus and mold collecting device according to the present invention.
  • the collecting device 10 includes an air inlet 12 for sucking external air into the body 11, an air passage 13 through which the sucked air passes, and an air outlet for discharging the sucked air to the outside of the body 11. 14 is formed, the body portion 11 is further provided with a pump 18 to suck the air through the air inlet 12 and to discharge the air to the air outlet (14).
  • an inlet 16 through which a collecting part such as a cotton swab 20 is input is formed to collect at least one of a virus and a mold from the sucked air.
  • the body portion 11 may be made of metal, plastic, or the like, and may be made of a cylindrical or columnar shape.
  • the air inlet 12 and the air passage 13 and the air outlet 14 which are formed in the body portion 11 are all connected to one, and the air inlet 12 is an inlet part to easily suck the outside air. It would be nice to have this widely expanded.
  • the air passage 13 and the air outlet 14 may be formed in a size and shape that allows the air introduced through the air inlet 12 to be discharged in a smooth flow.
  • the capacity of the pump 18 to intake air from the air inlet 12 and to discharge air to the air outlet 14 should be able to suck and exhaust the air flow rate to the level of 8 ⁇ 10 lpm (liter per minute) It will not be a problem wherever you are, as long as you can smoothly inhale and exhaust air.
  • one side of the body portion 11 is formed with an inlet 16 for collecting air, such as a cotton swab 20 to trap the virus is settled.
  • the inlet 16 also serves to guide the collector to be located in the air passage 13.
  • the inlet 16 may be located anywhere in the body 11, but it may be better to guide the collecting unit and the air flow path 13 to be positioned vertically. It's a good idea to place them where they can be collected.
  • 3 and 4 is a view of collecting the virus with the virus and the mold collecting device according to the present invention and the collected virus is measured with a measurement kit.
  • a collecting part is inserted into the collecting device 10 to collect viruses in the air.
  • the collecting part may use a separate collecting part included in the existing ATP kit, but may be used as a commercially available cotton swab 20. Using a swab 20, it will be able to collect the virus at a lower cost than using the existing kit.
  • the cotton ball 22 portion of the cotton swab 20 is inserted into the inlet 16 so that the air flow passage 13 faces, and then the pump 18 is operated so that viruses in the air can be collected on the cotton swab 20. .
  • the pump 18 may inhale air at a level of about 8 to 10 lpm (liter per minute), and even if the air is sucked at the lowest air flow rate of 8 lpm, it may sufficiently capture the virus.
  • the differential pressure of the air flow path 13 is reduced, which has the effect of reducing the energy consumed by the pump 18 during the operation of the collecting device 10.
  • the pump 18 is operated for a few minutes so that a sufficient amount of virus can be collected in the cotton ball 22 of the cotton swab 20, and then the pump 18 is stopped.
  • the operation of the pump 18 does not necessarily need to be performed for a predetermined time, but may be operated for a time sufficient to collect an amount that can be detected by the detection kit 40 or the agar plate 50.
  • the cotton swab 20 taken out from the inlet 16 to dissolve the virus is reacted with the reagent 32 prepared in advance in the container 22 to dissolve the virus. That is, the cotton swab 20 in which the virus is collected is placed in a container 22 containing 0.5 ml of the reagent 32, and rotated about 10 times so as to sufficiently react with the reagent 32.
  • 0.5 ml of reagent is a small amount of small protein that cuts the body of the virus consisting of the protein, the antigen attached to the cotton swab to a sufficient capacity to bind the antibody attached to the detection kit. Rotate a cotton swab to the reagent and stir it so that the virus antigen and antibody can bind enough to detect it.
  • the reagent 32 in which the virus is dissolved is dropped into the reagent inlet 42 of the detection kit 40 by about five drops. Drop the reagent 32 and wait until the detection kit 40 reacts for about 5 minutes. In general, the reagent 32 and the detection kit 40 should wait for about 8 minutes to fully react, but 5 minutes for rapid measurement will be sufficient. Next, the display window 44 is checked to determine the presence or absence of a virus.
  • virus contamination can be measured quickly in areas where virus contamination is suspected, and the damages are responded to according to the measurement results. Can be minimized.
  • the collection device 10 and the small amount of reagents 32 are easy to carry, can be confirmed the presence of viruses in the air with a minimum number of people, using a common cotton swab 20 rather than expensive dedicated kits This makes it possible to detect viruses at low cost.
  • Figure 5 relates to the appearance of measuring the mold collected by the virus and the mold collecting device according to the present invention.
  • Very fine size of mold spores are floating in the air, through the collecting device 10 can measure not only the virus but also the mold contained in the air.
  • the collection device 10 is coupled to a collecting unit such as a cotton swab 20 to collect the surrounding air to be measured, so that various dusts, bacteria, viruses and molds contained in the air adhere to the surface of the cotton swab 20. do. Then, the collecting device 10 and the cotton swab 20 are separated. Up to this point, the procedure for measuring the virus described above is performed in the same manner.
  • a collecting unit such as a cotton swab 20 to collect the surrounding air to be measured, so that various dusts, bacteria, viruses and molds contained in the air adhere to the surface of the cotton swab 20. do. Then, the collecting device 10 and the cotton swab 20 are separated. Up to this point, the procedure for measuring the virus described above is performed in the same manner.
  • a cotton swab 20 is rubbed onto the agar plate 50, which is a solid medium in which the mold can grow, to allow various substances such as mold to adhere onto the solid medium. If you rub the swab on the bacteria-only medium, only the bacteria grow, and if you rub the swab on the mold medium, only the mold grows.
  • the agar plate 50 used to measure the mold may be made by the user himself, and may be commercially available.

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Abstract

The present invention relates to a method for measuring and identifying viruses and mold in the air, the method comprising the steps of: coupling a collection part to a collection device; operating the collection device for a predetermined time to collect at least one of viruses and mold in the air in the collection part; separating the collection part from the collection device; and selecting whether to detect the viruses or the mold.

Description

공기 중 바이러스 및 곰팡이 측정 방법How to measure viruses and fungi in the air
본 발명은 공기 중 바이러스나 곰팡이의 유, 무를 판단하는 방법으로, 공기를 포집한 포집 장치로부터 분리된 포집부를 시약과 반응시켜, 시약의 변화를 통해 공기 중 바이러스 및 곰팡이의 유, 무를 측정하는 공기 중 바이러스 및 곰팡이 측정 방법에 관한 것이다.The present invention is a method for determining the presence or absence of viruses or mold in the air, by reacting the collection unit separated from the air collecting device with the reagent, the air to measure the presence or absence of viruses and mold in the air through the change of reagent It relates to a heavy virus and fungal measurement method.
공기 중에는 먼지, 미세먼지, 꽃가루 이외에도 많은 종류의 바이러스나 곰팡이가 포함되어 있으며, 매일 엄청난 수의 바이러스가 공기를 따라 이동하고 있다. 한 지역에서 발견된 바이러스가 거리가 매우 멀리 떨어진 지역에서도 유전적으로 유사한 바이러스가 발견되고 있다. 이는 바이러스가 먼지와 함께 공기 중으로 들어가 수천 km를 이동할 수 있다는 것을 보여주고 있으며, 현재 바이러스가 발생하지 않았다고 해서 그 지역이 항상 안전한 것은 아니다.In addition to dust, fine dust and pollen, the air contains many kinds of viruses and fungi, and huge numbers of viruses move along the air every day. Viruses found in one region are genetically similar, even in very remote areas. This shows that viruses can move into the air with dust and travel thousands of kilometers, and the area is not always safe just because a virus is not present.
일반적으로 대부분의 바이러스 및 곰팡이는 큰 문제를 일으키지 않지만, 몇몇 바이러스 및 곰팡이의 경우, 치명적인 질병을 유발하여 어느 지역의 한 생명체를 완전히 초토화 시킬 수도 있기 때문에 안심할 수 없으며, 항상 주의해야 한다. In general, most viruses and fungi don't cause major problems, but some viruses and fungi can cause fatal illnesses that can completely destroy an organism in a given area and should always be used with caution.
따라서 이와 같이 바이러스 및 곰팡이의 발생이 의심되는 지역의 바이러스 유, 무를 검지하기 위한 장치 및 방법이 다수 제안되어 있다.Therefore, a number of devices and methods for detecting the presence or absence of viruses in areas where viruses and molds are suspected have been proposed.
종래의 공기 중의 부유 바이러스를 확인하는 방법으로는 공기를 물과 접촉시켜 수용액으로 바이러스를 포집시킨 후, 이를 면역반응이나 DNA를 분석하는 방법으로 확인하였으며, 다른 방법으로는 공조기의 필터 상에 흡착된 생체 물질을 추출하여 분석하는 방법을 사용하고 있다. 하지만 공기를 물과 접촉시켜서 수용액 상으로 바이러스를 포집시키는 방법이나 공조기의 필터를 이용하는 방법은 공조 시스템을 가동해야 하기 때문에 시스템 규모가 커지고, 많은 에너지를 소비하게 된다.Conventional airborne virus was identified by contacting air with water to collect the virus in aqueous solution, and then analyzing it by immunoreaction or DNA analysis. The method of extracting and analyzing biomaterials is used. However, the method of collecting the virus in the aqueous solution by contacting air with water or using the air conditioner's filter requires the operation of the air conditioning system, which increases the system size and consumes a lot of energy.
또 다른 예로, 한국 등록특허 공보 제10-1269173호(등록일자: 2013.05.23)은 박막 집적 전자소자를 이용한 박테리아 및 바이러스의 측정방법 및 측정장치에 관한 것으로, 생체 고분자 물질이 집적된 박막과 전극이 통합한 전자 소자를 이용하여, 박테리아의 대사 작용 및 바이러스 유래 효소 반응에 의한 박막의 전기 화학적 변화를 측정하여 박테리아 및 바이러스의 유무 및 농도를 측정한다. As another example, Korean Patent Publication No. 10-1269173 (Registration Date: 2013.05.23) relates to a method and apparatus for measuring bacteria and viruses using a thin film integrated electronic device. Using this integrated electronic device, the electrochemical change of the thin film due to the metabolic action of the bacteria and the enzyme reaction derived from the virus is measured to determine the presence and concentration of bacteria and viruses.
본 발명은 저렴한 비용으로 공기 중 바이러스 및 곰팡이를 검지할 수 있는 방법을 제공하고자 한다.The present invention is to provide a method for detecting viruses and mold in the air at a low cost.
본 발명은 바이러스 및 곰팡이 오염이 의심되는 지역에서 신속하게 바이러스 및 곰팡이를 검지할 수 있는 방법을 제공하고자 한다.The present invention is to provide a method that can quickly detect the virus and mold in areas where virus and mold contamination is suspected.
이러한 목적을 달성하기 위한 본 발명은 공기 중 바이러스 및 곰팡이 측정 방법으로, 포집 장치에 포집부를 결합하는 단계; 상기 포집 장치를 기설정된 시간 동안 작동시켜 공기 중 바이러스 및 곰팡이 가운데 적어도 하나를 상기 포집부에 포집하는 단계; 상기 포집 장치와 상기 포집부를 분리하는 단계; 및 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계; 를 포함하고, 상기 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계에서, 바이러스 검지를 선택하는 경우에는 상기 포집부를 시약이 구비되어 있는 용기에 투입시켜, 상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계; 바이러스가 용해된 상기 시약을 키트에 기설정된 시간 동안 반응시키는 단계; 상기 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계; 를 거쳐 바이러스를 측정하며, 상기 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계 에서, 곰팡이 검지를 선택하는 경우에는 상기 포집부를 곰팡이가 성장할 수 있는 아가플레이트에 문질러주는 단계; 상기 아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계; 및 상기 배양기에서 상기 아가플레이트를 꺼내 곰팡이를 측정하는 단계; 를 거쳐 곰팡이를 측정할 수 있다. The present invention for achieving this object is a method for measuring viruses and fungi in the air, comprising the steps of combining the collecting unit to the collecting device; Operating the collecting device for a predetermined time to collect at least one of viruses and fungi in the air in the collecting unit; Separating the collecting device and the collecting part; And selecting whether to detect a virus or mold. In the step of selecting whether to detect the virus or mold detection, in the case of detecting the virus, the collection unit is put in a container provided with a reagent, the virus collected in the collection unit Dissolving with the reagents; Reacting the reagent in which the virus is dissolved in the kit for a predetermined time; Determining the presence or absence of a virus through a change in the kit; Measuring the virus through and selecting whether to detect the virus or the mold, and when the detection of the mold is selected, rubbing the collection unit to an agar plate on which mold can grow; Storing the agar plates in the incubator for a predetermined time; And taking out the aga plate in the incubator to measure the mold; The mold can be measured via.
바람직하게는 본 발명에 있어서, 상기 포집 장치와 상기 포집부를 결합하는 단계에서, 상기 포집부는 면봉으로, 상기 면봉의 솜뭉치 부분이 상기 포집 장치의 공기 유로에 위치한다.Preferably in the present invention, in the step of combining the collecting device and the collecting unit, the collecting unit is a cotton swab, the cotton ball portion of the cotton swab is located in the air flow path of the collecting device.
바람직하게는 본 발명에 있어서, 상기 포집 장치로 공기를 포집하는 단계에서, 상기 포집 장치가 공기를 8-10lpm(liter per minute)의 유량으로 포집한다.Preferably, in the present invention, in the step of collecting air with the collecting device, the collecting device collects air at a flow rate of 8-10 lpm (liter per minute).
바람직하게는 본 발명에 있어서, 상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계에서, 상기 포집부를 0.5ml의 상기 시약에 투입하고, 10회 회전시켜 바이러스를 용해시킨다.Preferably, in the present invention, in the step of dissolving the virus collected in the collecting unit with the reagent, the collecting unit is added to 0.5 ml of the reagent and rotated 10 times to dissolve the virus.
바람직하게는 본 발명에 있어서, 바이러스가 용해된 상기 시약을 키트에 시설정된 시간 동안 반응시키는 단계에서, 상기 키트에 바이러스가 용해된 상기 시약을 다섯 방울 떨어트린다.Preferably, in the present invention, in the step of reacting the reagent in which the virus is dissolved in the kit for a predetermined time, five drops of the reagent in which the virus is dissolved in the kit are dropped.
바람직하게는 본 발명에 있어서, 바이러스가 용해된 상기 시약을 키트에 기설정된 시간 동안 반응시키는 단계에서, 상기 키트와 바이러스가 용해된 상기 시약을 5~8분 동안 반응시킨다.Preferably, in the present invention, in the step of reacting the reagent in which the virus is dissolved in the kit for a predetermined time, the kit and the reagent in which the virus is dissolved are reacted for 5 to 8 minutes.
바람직하게는 본 발명에 있어서, 상기 아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계에서, 상기 아가플레이트를 상기 배양기에 24시간동안 보관한다.Preferably, in the present invention, in the step of storing the agar plate in the incubator for a predetermined time, the agar plate is stored in the incubator for 24 hours.
바람직하게는 본 발명에 있어서, 포집 장치에 포집부를 결합하는 단계와 상기 포집 장치를 기설정된 시간 동안 작동시켜 공기 중 바이러스를 상기 포집부에 포집하는 단계와 상기 포집 장치와 상기 포집부를 분리하는 단계와 바이러스 또는 곰팡이 검지 단계를 선택하는 단계와 상기 바이러스 또는 곰팡이 검지 단계를 선택하는 단계에서 바이러스 검지를 선택하여, 상기 포집부를 시약 0.5ml가 구비되어 있는 용기에 투입하고 10회 회전시켜, 상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계와 바이러스가 용해된 상기 시약을 키트에 다섯 방울 떨어트리고, 5~8분 동안 반응시키는 단계와 상기 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계 또는 상기 바이러스 또는 곰팡이 검지 단계를 선택하는 단계에서 곰팡이 검지를 선택하여, 상기 포집부를 곰팡이가 성장할 수 있는 아가플레이트에 문질러주는 단계와 상기 아가플레이트를 24시간동안 배양기에 보관하는 단계 및 상기 배양기에서 상기 아가플레이트를 꺼내 곰팡이를 측정하는 단계;를 거쳐 곰팡이를 측정할 수 있다.Preferably, in the present invention, the step of coupling the collecting unit to the collecting device, operating the collecting device for a predetermined time to collect the air virus in the collecting unit and separating the collecting device and the collecting unit; In the step of selecting the virus or mold detection step and the step of detecting the virus or fungus, the virus detection is selected, the collecting part is put in a container equipped with 0.5 ml of the reagent and rotated 10 times. Dissolving the collected virus with the reagent, dropping the reagent in which the virus is dissolved into the kit, reacting for 5-8 minutes, and determining the presence or absence of the virus by changing the kit or the In the step of selecting a virus or fungus detection step, Rubbing the collection unit on the agar plate that can grow mold, and storing the agar plate in the incubator for 24 hours and taking out the aga plate from the incubator to measure the mold; can be measured through .
바람직하게는 본 발명에 있어서, 상기 포집 장치로 공기를 포집하는 단계에서, 상기 포집 장치가 공기를 8-10lpm(liter per minute)의 유량으로 포집한다.Preferably, in the present invention, in the step of collecting air with the collecting device, the collecting device collects air at a flow rate of 8-10 lpm (liter per minute).
본 발명에 따른 공기 중 바이러스 및 곰팡이 측정 방법은 고가의 전용 키트를 구비할 필요없이, 일반 면봉을 사용하여 저렴한 비용으로 바이러스와 곰팡이를 검지하는 효과가 있다.The virus and mold measurement method in the air according to the present invention has the effect of detecting viruses and molds at low cost by using a general cotton swab, without having to provide an expensive dedicated kit.
본 발명은 짧은 시간동안 포집된 공기 속에서 바이러스 및 곰팡이를 검지하여 신속하게 바이러스 및 곰팡이의 유, 무를 확인할 수 있다.The present invention can quickly detect the presence or absence of viruses and fungi by detecting viruses and fungi in the collected air for a short time.
도 1은 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 바이러스와 곰팡이를 측정하는 과정에 대한 순서도,1 is a flow chart for the process of measuring the virus and fungi with the virus and mold collecting device according to the present invention,
도 2는 본 발명에 따른 바이러스 및 곰팡이 포집 장치에 대한 정면도,Figure 2 is a front view of the virus and mold collecting device according to the present invention,
도 3은 본 발명에 따른 바이러스 및 곰팡이 포집 장치를 사용하고 있는 모습,3 is a view using a virus and mold collecting device according to the present invention,
도 4는 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 포집된 바이러스를 측정하는 모습,4 is a view of measuring the virus collected by the virus and the mold collecting device according to the present invention,
도 5는 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 포집된 곰팡이를 측정하는 모습.Figure 5 is a measure of the fungi collected by the virus and the mold collecting device according to the invention.
본 발명의 실시예에서 제시되는 특정한 구조 내지 기능적 설명들은 단지 본 발명의 개념에 따른 실시예를 설명하기 위한 목적으로 예시된 것으로, 본 발명의 개념에 따른 실시예들은 다양한 형태로 실시될 수 있다. 또한 본 명세서에 설명된 실시예들에 한정되는 것으로 해석되어서는 아니 되며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변경물, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다.Specific structural or functional descriptions presented in the embodiments of the present invention are only illustrated for the purpose of describing the embodiments according to the inventive concept, and the embodiments according to the inventive concept may be implemented in various forms. In addition, it should not be construed as limited to the embodiments described herein, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention.
이하에서는 첨부된 도면을 참조하여 본 발명에 대해 상세히 설명한다.Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the present invention.
도 1은 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 바이러스와 곰팡이를 측정하는 과정에 대한 순서도를 보여주고 있다.Figure 1 shows a flow chart for the process of measuring viruses and fungi with the virus and fungi collection device according to the present invention.
본 발명의 실시예에 의한 공기 중 바이러스 및 곰팡이 측정 방법은, 포집 장치에 포집부를 결합하는 단계(S10)와, 포집 장치를 작동시켜 공기 중 바이러스 및 곰팡이 가운데 적어도 하나를 포집부에 포집하는 단계(S20)와, 포집 장치와 포집부를 분리하는 단계(S30)와, 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계(S40)를 포함한다.In the air virus and mold measurement method according to an embodiment of the present invention, the step (S10) for coupling the collecting unit to the collecting device, and operating the collecting device to collect at least one of the virus and mold in the air to the collecting unit ( S20), separating the collecting device and the collecting unit (S30), and selecting whether to detect a virus or a mold (S40).
포집 장치에 포집부를 결합하는 단계(S10)에서, 포집부는 면봉(20)으로, 면봉(20)의 솜뭉치(22) 부분이 바이러스 및 곰팡이 포집 장치(10) (이하, '포집 장치'라 함)의 공기 유로(13)에 위치하도록 한다.In the step (S10) of combining the collecting unit to the collecting device, the collecting unit is a cotton swab 20, the cotton ball 22 of the cotton swab 20 is a virus and mold collecting device 10 (hereinafter, referred to as 'collecting device' ) In the air flow path 13.
포집 장치(10)를 작동시켜 공기 중 바이러스 및 곰팡이 가운데 적어도 하나를 포집부에 포집하는 단계(S20)에서, 포집 장치(10)를 기설정된 시간 동안 작동시켜, 공기 중에 포함되어 있는 바이러스와 곰팡이가 포집부에 포집되게 한다.In operation S20 of collecting at least one of air virus and mold in the collecting unit by operating the collecting device 10, the collecting device 10 is operated for a predetermined time so that viruses and mold contained in the air Allow collection to be collected.
바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계(S40)에서 바이러스의 검지를 선택하는 경우에는, 포집부를 시약이 구비되어 있는 용기에 투입시켜, 포집부에 포집된 바이러스를 시약으로 용해하는 단계(S50)와 바이러스가 용해된 시약을 키트에 묻혀 검지하는 단계(S60) 및 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계(S70)를 추가적으로 진행할 수 있다.When detecting the virus in step S40 of selecting whether to detect a virus or a mold, the collection part is put into a container equipped with a reagent, and the virus collected in the collection part is dissolved in a reagent. The step (S50) and the step of detecting the virus-dissolved reagent in the kit (S60) and the change of the kit through the change of the kit (S70) can be further proceeded.
포집부를 시약이 구비되어 있는 용기에 투입시켜, 포집부에 포집된 바이러스를 시약으로 용해하는 단계(S50)에서는, 시약(32)에 투입된 면봉(20)을 다수 휘저어 바이러스를 용해시키며, 바람직하게는 약 10회 회전시켜 면봉(20)에 포집된 바이러스와 시약(32)이 충분히 반응할 수 있도록 한다.In the collecting unit into a container provided with a reagent, and dissolving the virus collected in the collecting unit as a reagent (S50), a plurality of swabs 20 added to the reagent 32 are stirred to dissolve the virus. Rotate about 10 times to allow the virus collected on the swab 20 to react sufficiently with the reagent 32.
바이러스가 용해된 시약을 키트에 묻혀 검지하는 단계(S60)에서 바이러스가 용해된 시약(32)을 검지키트(40)에 묻히고, 기설정된 시간 동안 반응시킨다. 약 5~8분간 시약(32)과 검지키트(40)가 충분히 반응할 때까지 기다리며, 바람직하게는 5분간 반응시켜도 충분할 것이다.In the step of detecting the virus-dissolved reagent in the kit (S60), the virus-dissolved reagent 32 is buried in the detection kit 40 and reacted for a predetermined time. Waiting for about 5-8 minutes for the reagent 32 and the detection kit 40 to sufficiently react, preferably 5 minutes.
마지막으로, 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계(S70)에서 검지키트(40)의 표시창(44) 변화를 보고 공기 중의 바이러스 존재를 파악하면 될 것이다.Finally, the step of determining the presence or absence of the virus through the change of the kit (S70) to see the change in the display window 44 of the detection kit 40 may be to determine the presence of the virus in the air.
이와 같은 단계를 거쳐 공기 중 바이러스 유, 무를 간편하게 확인할 수 있으며, 바이러스가 검지 되었으면, 공기 중 바이러스 정량화를 위해 정밀 분석 인원을 투입하면 될 것이다.Through these steps, the presence or absence of virus in the air can be easily checked. If a virus is detected, a precise analysis staff can be put in to quantify the virus in the air.
한편, 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계(S40)에서 곰팡이의 검지를 선택하는 경우에는, 포집 장치에 포집부를 결합하는 단계(S10)와 포집 장치를 기설정된 시간 동안 작동시켜 공기 중 곰팡이를 포집부에 포집하는 단계(S20)와 포집 장치와 포집부를 분리하는 단계(S30)까지는 바이러스 검지 과정과 동일하게 이루어진다. 하지만 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계(S40)에서 곰팡이의 검지를 선택한 이후부터는 바이러스 검지 과정과 다른 과정으로 곰팡이를 점지하게 된다.On the other hand, when the detection of the mold is selected in the step (S40) of selecting whether to detect the virus or mold, the step of coupling the collecting unit to the collecting device (S10) and operating the collecting device for a predetermined time In order to collect the mold in the air to the collecting unit (S20) and separating the collecting device and the collecting unit (S30) is made in the same manner as the virus detection process. However, after selecting the detection of the mold in the step (S40) of selecting whether to detect the virus or the mold, the mold is occupied by a process different from the virus detection process.
바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계(S40)에서 곰팡이의 검지를 선택하는 경우에는, 포집부를 곰팡이가 자랄 수 있는 아가플레이트에 문질러주는 단계(S51)와 아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계(S61) 및 배양기에서 아가플레이트를 꺼내 곰팡이를 측정하는 단계(S71)를 추가적으로 진행할 수 있다.If the detection of the mold is selected in the step of selecting whether to detect the virus or the mold (S40), the step of rubbing the collecting part on the agar plate that can grow mold (S51) and the aga plate Storage in the incubator for a time (S61) and take out the agar plate from the incubator to measure the mold (S71) can be further proceeded.
포집부를 곰팡이가 자랄 수 있는 아가플레이트에 문질러주는 단계(S51)에서 포집부인 면봉(20)에 묻어있는 곰팡이 포자를 아가플레이트(50)에 묻혀주게 된다. In the step (S51) to rub the collecting part on the agar plate that mold can grow mold spores buried in the cotton swab 20 is collected in the agar plate 50.
아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계(S61)는 곰팡이 포자가 성장할 수 있는 최적의 조건을 제공하여 배양기에서 충분히 성장할 수 있도록 한다.The step of storing the agar plate in the incubator for a predetermined time (S61) provides the optimum conditions for the growth of the fungal spores to allow sufficient growth in the incubator.
그리고 마지막으로 배양기에서 아가플레이트를 꺼내 곰팡이를 측정하는 단계(S71)를 통해 공기 중 곰팡이가 얼마나 포함되어 있는지 아가플레이트(50)에서 자라난 곰팡이의 개수를 확인하면 된다.And finally, by taking out the agar plate from the incubator and measuring the mold (S71), the number of molds grown in the agar plate 50 may be determined how much mold is contained in the air.
도 2는 본 발명에 따른 바이러스 및 곰팡이 포집 장치에 대한 정면도에 관한 것이다.Figure 2 relates to a front view of the virus and mold collecting device according to the present invention.
포집 장치(10)는 몸체부(11)에 외부 공기를 흡입하는 공기 흡입구(12)와 흡입된 공기가 지나가는 공기 유로(13) 및 흡입된 공기를 몸체부(11)의 외부로 내보내는 공기 배출구(14)가 형성되어 있으며, 몸체부(11) 내부에는 공기 흡입구(12)를 통해 공기를 흡입하고 공기 배출구(14)로 공기를 배출할 수 있도록 하는 펌프(18)가 더 구비되어 있다. 또한 흡입된 공기로부터 바이러스 및 곰팡이 가운데 적어도 하나를 채취할 수 있도록 면봉(20)과 같은 포집부가 투입되는 투입구(16)가 형성되어 있다.The collecting device 10 includes an air inlet 12 for sucking external air into the body 11, an air passage 13 through which the sucked air passes, and an air outlet for discharging the sucked air to the outside of the body 11. 14 is formed, the body portion 11 is further provided with a pump 18 to suck the air through the air inlet 12 and to discharge the air to the air outlet (14). In addition, an inlet 16 through which a collecting part such as a cotton swab 20 is input is formed to collect at least one of a virus and a mold from the sucked air.
몸체부(11)는 금속이나 플라스틱 등으로 제작될 수 있고, 원통형 또는 기둥형 등으로 이루어질 수 있다.The body portion 11 may be made of metal, plastic, or the like, and may be made of a cylindrical or columnar shape.
몸체부(11) 내부에 형성되어 있는 공기 흡입구(12)와 공기 유로(13) 및 공기 배출구(14)는 모두 하나로 연결되어 있으며, 공기 흡입구(12)는 외부의 공기를 흡입하기 용이하도록 입구 부분이 넓게 확장되어 있는 것이 좋을 것이다.The air inlet 12 and the air passage 13 and the air outlet 14 which are formed in the body portion 11 are all connected to one, and the air inlet 12 is an inlet part to easily suck the outside air. It would be nice to have this widely expanded.
공기 유로(13)와 공기 배출구(14)는 공기 흡입구(12)를 통해 유입된 공기가 원활한 흐름으로 지나가며 배출될 수 있는 크기와 형태로 이루어지는 것이 좋을 것이다.The air passage 13 and the air outlet 14 may be formed in a size and shape that allows the air introduced through the air inlet 12 to be discharged in a smooth flow.
한편, 공기 흡입구(12)로부터 공기를 흡입하고 공기 배출구(14)로 공기를 배출하는 펌프(18)의 용량은 공기 유량을 8~10lpm(liter per minute)의 수준으로 흡, 배기할 수 있어야 할 것이며, 원만하게 공기의 흡입과 배출을 할 수 있다면 어느 곳에 위치해도 문제되지 않을 것이다.On the other hand, the capacity of the pump 18 to intake air from the air inlet 12 and to discharge air to the air outlet 14 should be able to suck and exhaust the air flow rate to the level of 8 ~ 10 lpm (liter per minute) It will not be a problem wherever you are, as long as you can smoothly inhale and exhaust air.
또한 몸체부(11)의 일측면에는 공기를 포집하여 바이러스가 안착하게 되는 면봉(20)과 같은 포집부를 투입하는 투입구(16)가 형성되어 있다. 투입구(16)는 포집부가 공기 유로(13)에 위치할 수 있도록 가이드하는 역할을 하기도 한다. 투입구(16)는 몸체부(11)의 어느 곳에 위치하더라도 크게 상관 없겠지만, 포집부와 공기 유로(13)가 수직으로 위치할 수 있도록 가이드하는 것이 좋을 것이며, 바람직하게는 포집부에 바이러스 및 곰팡이가 잘 포집될 수 있는 곳으로 위치하는 것이 좋을 것이다.In addition, one side of the body portion 11 is formed with an inlet 16 for collecting air, such as a cotton swab 20 to trap the virus is settled. The inlet 16 also serves to guide the collector to be located in the air passage 13. The inlet 16 may be located anywhere in the body 11, but it may be better to guide the collecting unit and the air flow path 13 to be positioned vertically. It's a good idea to place them where they can be collected.
도 3과 도4는 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 바이러스를 포집하는 모습과 포집된 바이러스를 측정 키트로 측정하는 모습이다.3 and 4 is a view of collecting the virus with the virus and the mold collecting device according to the present invention and the collected virus is measured with a measurement kit.
바이러스를 포집하고, 포집된 바이러스를 측정하는 과정을 살펴보면, 크게 공기 중 바이러스를 포집하고, 바이러스 용해 및 바이러스 검지하는 과정으로 이루어진다.Looking at the process of collecting the virus, and measuring the collected virus, it is largely composed of the process of collecting the virus in the air, virus lysis and virus detection.
공기 중 바이러스를 포집하는 과정에서 공기 중 바이러스를 포집하기 위해 포집 장치(10)에 포집부를 삽입한다. In the process of collecting viruses in the air, a collecting part is inserted into the collecting device 10 to collect viruses in the air.
포집부는 기존의 ATP 키트에 포함되어 있는 별도의 포집부를 사용할 수도 있겠지만, 시중에 판매하고 있는 면봉(20)으로 사용해도 무방할 것이다. 면봉(20)을 사용하게 되면, 기존의 키트를 사용할 때 보다 저렴한 비용으로 바이러스를 포집할 수 있을 것이다.The collecting part may use a separate collecting part included in the existing ATP kit, but may be used as a commercially available cotton swab 20. Using a swab 20, it will be able to collect the virus at a lower cost than using the existing kit.
면봉(20)의 솜뭉치(22) 부분이 공기 유로(13)를 향하도록 투입구(16)에 삽입시킨 다음, 펌프(18)를 작동시켜 공기 중의 바이러스가 면봉(20)에 포집될 수 있도록 한다.The cotton ball 22 portion of the cotton swab 20 is inserted into the inlet 16 so that the air flow passage 13 faces, and then the pump 18 is operated so that viruses in the air can be collected on the cotton swab 20. .
펌프(18)는 대략 8~10lpm(liter per minute) 수준으로 공기를 흡입하게 되며, 최저 공기 유량인 8lpm으로 공기를 흡입을 하더라도, 바이러스를 충분히 포집할 수 있다. 공기를 흡입하는 유량이 감소하게 되면, 공기 유로(13)의 차압이 감소하게 되며, 이는 포집 장치(10)의 작동시 펌프(18)에서 소비하는 에너지를 절감하는 효과가 있다.The pump 18 may inhale air at a level of about 8 to 10 lpm (liter per minute), and even if the air is sucked at the lowest air flow rate of 8 lpm, it may sufficiently capture the virus. When the flow rate for sucking air is reduced, the differential pressure of the air flow path 13 is reduced, which has the effect of reducing the energy consumed by the pump 18 during the operation of the collecting device 10.
펌프(18)를 수분간 작동시켜, 면봉(20)의 솜뭉치(22)에 충분한 양의 바이러스가 포집될 수 있도록 한 다음, 펌프(18)의 작동을 중단한다. 펌프(18)의 작동은 반드시 정해진 시간동안 해야할 필요는 없으며, 검지키트(40)나 아가플레이트(50)에 검지될 수 있을 정도의 양을 포집하기 알맞은 시간동안 작동시키면 될 것이다.The pump 18 is operated for a few minutes so that a sufficient amount of virus can be collected in the cotton ball 22 of the cotton swab 20, and then the pump 18 is stopped. The operation of the pump 18 does not necessarily need to be performed for a predetermined time, but may be operated for a time sufficient to collect an amount that can be detected by the detection kit 40 or the agar plate 50.
그 다음 바이러스를 용해시키기 위해 투입구(16)로부터 꺼낸 면봉(20)을 용기(22)에 미리 준비해둔 시약(32)과 반응시켜 바이러스를 용해한다. 즉, 0.5ml의 시약(32)이 담겨있는 용기(22)에 바이러스가 포집된 면봉(20)을 넣고, 약 10회 정도 회전시켜 시약(32)과 충분히 반응할 수 있도록 한다. Then, the cotton swab 20 taken out from the inlet 16 to dissolve the virus is reacted with the reagent 32 prepared in advance in the container 22 to dissolve the virus. That is, the cotton swab 20 in which the virus is collected is placed in a container 22 containing 0.5 ml of the reagent 32, and rotated about 10 times so as to sufficiently react with the reagent 32.
0.5ml의 시약은 면봉에 붙어있는 항원인 단백질로 구성된 바이러스의 몸통을 작게 짤라 수 많은 작은 단백질의 형태로 만들어 검지키트에 붙어있는 항체와 충분히 결합할 수 있도록 하는 용량이다. 그리고 시약에 면봉을 충분히 회전시켜 저어줘 바이러스의 항원과 항체가 충분히 결합해 검지가 잘될 수 있도록 한다.0.5 ml of reagent is a small amount of small protein that cuts the body of the virus consisting of the protein, the antigen attached to the cotton swab to a sufficient capacity to bind the antibody attached to the detection kit. Rotate a cotton swab to the reagent and stir it so that the virus antigen and antibody can bind enough to detect it.
마지막으로 바이러스를 검지하기 위해서 바이러스가 용해된 시약(32)을 검지키트(40)의 시약 투입구(42)에 약 다섯 방울로 약 100uL을 떨어뜨린다. 시약(32)을 떨어뜨린 후 약 5분간 검지키트(40)가 반응할 때까지 기다린다. 일반적으로 시약(32)과 검지키트(40)가 충분히 반응할 수 있도록 약 8분간 기다려야 하지만, 신속한 측정을 위해 5분간 반응시켜도 충분할 것이다. 그 다음, 표시창(44)을 확인하여 바이러스 유, 무를 판단한다.Finally, in order to detect the virus, about 100 uL of the reagent 32 in which the virus is dissolved is dropped into the reagent inlet 42 of the detection kit 40 by about five drops. Drop the reagent 32 and wait until the detection kit 40 reacts for about 5 minutes. In general, the reagent 32 and the detection kit 40 should wait for about 8 minutes to fully react, but 5 minutes for rapid measurement will be sufficient. Next, the display window 44 is checked to determine the presence or absence of a virus.
공기를 포집하고, 시약(32)을 통해 바이러스 유, 무를 판단하기까지 약 10분 정도의 시간이 소요되기 때문에 바이러스 오염이 의심되는 지역에서 신속하게 바이러스 오염을 측정하고, 측정 결과에 따라 대응하여 피해를 최소화할 수 있다. 또한 포집 장치(10)와 적은 양의 시약(32)은 휴대가 간편하고, 최소한의 인원으로 공기 중 바이러스 존재를 확인할 수 있으며, 고가의 전용 키트가 아닌 일반적으로 사용하는 면봉(20)을 사용하기 때문에 저렴한 비용으로 바이러스를 검지할 수 있다.Since it takes about 10 minutes to collect air and determine the presence or absence of virus through the reagents (32), virus contamination can be measured quickly in areas where virus contamination is suspected, and the damages are responded to according to the measurement results. Can be minimized. In addition, the collection device 10 and the small amount of reagents 32 are easy to carry, can be confirmed the presence of viruses in the air with a minimum number of people, using a common cotton swab 20 rather than expensive dedicated kits This makes it possible to detect viruses at low cost.
도 5는 본 발명에 따른 바이러스 및 곰팡이 포집 장치로 포집된 곰팡이를 측정하는 모습에 관한 것이다.Figure 5 relates to the appearance of measuring the mold collected by the virus and the mold collecting device according to the present invention.
매우 미세한 크기의 곰팡이 포자는 공기 중에 떠다니고 있어, 포집 장치(10)를 통해 바이러스뿐만 아니라 공기 중에 포함되어 있는 곰팡이도 측정할 수 있다. Very fine size of mold spores are floating in the air, through the collecting device 10 can measure not only the virus but also the mold contained in the air.
포집 장치(10)에 면봉(20)과 같은 포집부를 결합하여 측정하고자 하는 주변의 공기를 포집시켜, 공기에 포함되어 있는 각종 먼지, 세균, 바이러스 및 곰팡이 등이 면봉(20)의 표면에 부착되도록 한다. 그리고 포집 장치(10)와 면봉(20)을 분리시킨다. 여기까지는 상기에서 설명한 바이러스를 측정하기 위한 과정과 동일하게 이루어진다. The collection device 10 is coupled to a collecting unit such as a cotton swab 20 to collect the surrounding air to be measured, so that various dusts, bacteria, viruses and molds contained in the air adhere to the surface of the cotton swab 20. do. Then, the collecting device 10 and the cotton swab 20 are separated. Up to this point, the procedure for measuring the virus described above is performed in the same manner.
곰팡이를 정량화하기 위해서는 곰팡이가 자랄 수 있는 고체 배지인 아가플레이트(50)에 면봉(20)을 문질러 고체 배지 위에 곰팡이 등 여러 물질이 부착할 수 있도록 한다. 만일 박테리아 전용 배지에 면봉을 문지르게 되면 박테리아만 자라게 되고, 곰팡이 배지에 면봉을 문지르게 되면 곰팡이만 자라게 된다.In order to quantify the mold, a cotton swab 20 is rubbed onto the agar plate 50, which is a solid medium in which the mold can grow, to allow various substances such as mold to adhere onto the solid medium. If you rub the swab on the bacteria-only medium, only the bacteria grow, and if you rub the swab on the mold medium, only the mold grows.
배지 위에 면봉(20)을 충분히 문질러 준 다음, 아가플레이트(50)를 배양기(미도시)에 24시간동안 보관한다. 그리고 아가플레이트(50)에서 자라난 곰팡이의 개수를확인하여 공기 중에 곰팡이가 얼마나 함유되어 있는지 측정할 수 있다.Rub the cotton swab 20 sufficiently on the medium, and then store the agar plate 50 in an incubator (not shown) for 24 hours. The number of molds grown on the agar plate 50 may be checked to determine how much mold is contained in the air.
곰팡이 측정에 사용되는 아가플레이트(50)는 사용자가 직접 만들어 사용할 수 있을 것이며, 시중에 판매하는 것을 사용할 수도 있을 것이다.The agar plate 50 used to measure the mold may be made by the user himself, and may be commercially available.
이상에서 설명한 본 발명은 전술한 실시예 및 첨부된 도면에 의해 한정되는 것이 아니고, 본 발명의 기술적 사상을 벗어나지 않는 범위 내에서 여러 가지 치환, 변형 및 변경이 가능함은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 명백할 것이다.The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

Claims (9)

  1. 포집 장치에 포집부를 결합하는 단계;Coupling the collecting unit to the collecting device;
    상기 포집 장치를 기설정된 시간 동안 작동시켜 공기 중 바이러스 및 곰팡이 가운데 적어도 하나를 상기 포집부에 포집하는 단계;Operating the collecting device for a predetermined time to collect at least one of viruses and fungi in the air in the collecting unit;
    상기 포집 장치와 상기 포집부를 분리하는 단계; 및Separating the collecting device and the collecting part; And
    바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계; Selecting whether to detect a virus or mold;
    를 포함하고, Including,
    상기 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계에서, 바이러스 검지를 선택하는 경우에는 In the step of selecting whether to detect the virus or the mold, when detecting the virus
    상기 포집부를 시약이 구비되어 있는 용기에 투입시켜, 상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계;Injecting the collecting unit into a container equipped with a reagent to dissolve the virus collected in the collecting unit with the reagent;
    바이러스가 용해된 상기 시약을 키트에 기설정된 시간 동안 반응시키는 단계;Reacting the reagent in which the virus is dissolved in the kit for a predetermined time;
    상기 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계;Determining the presence or absence of a virus through a change in the kit;
    를 거쳐 바이러스를 측정하며,Measures viruses via
    상기 바이러스를 검지할 것인지 또는 곰팡이를 검지할 것인지를 선택하는 단계 에서, 곰팡이 검지를 선택하는 경우에는 In the step of selecting whether to detect the virus or mold, if the mold detection is selected
    상기 포집부를 곰팡이가 성장할 수 있는 아가플레이트에 문질러주는 단계;Rubbing the collection part on an agar plate on which mold can grow;
    상기 아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계; 및Storing the agar plates in the incubator for a predetermined time; And
    상기 배양기에서 상기 아가플레이트를 꺼내 곰팡이를 측정하는 단계;Removing the agar plate from the incubator and measuring mold;
    를 거쳐 곰팡이를 측정하는 공기 중 바이러스 및 곰팡이 측정 방법.Methods of measuring viruses and fungi in the air to measure mold via
  2. 제1항에 있어서,The method of claim 1,
    상기 포집 장치와 상기 포집부를 결합하는 단계에서, 상기 포집부는 면봉으로, 상기 면봉의 솜뭉치 부분이 상기 포집 장치의 공기 유로에 위치하는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of coupling the collecting device and the collecting unit, the collecting unit is a cotton swab, the cotton ball portion of the cotton swab virus and mold measuring method in the air is located in the air flow path of the collecting device.
  3. 제1항에 있어서,The method of claim 1,
    상기 포집 장치로 공기를 포집하는 단계에서, 상기 포집 장치가 공기를 8-10lpm(liter per minute)의 유량으로 포집하는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of collecting the air to the collecting device, the virus and mold measuring method in the air collecting the air at a flow rate of 8-10 lpm (liter per minute).
  4. 제1항에 있어서,The method of claim 1,
    상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계에서, 상기 포집부를 0.5ml의 상기 시약에 투입하고, 10회 회전시켜 바이러스를 용해시키는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of dissolving the virus collected in the collecting part with the reagent, the collecting part is added to 0.5 ml of the reagent, and rotated 10 times to dissolve the virus virus and mold measurement method in the air.
  5. 제1항에 있어서,The method of claim 1,
    바이러스가 용해된 상기 시약을 키트에 시설정된 시간 동안 반응시키는 단계 에서, 상기 키트에 바이러스가 용해된 상기 시약을 다섯 방울 떨어트리는 공기 중 바이러스 및 곰팡이 측정 방법.Reacting the virus-dissolved reagents in the kit for a predetermined time, dropping five drops of the virus-dissolved reagents in the kit.
  6. 제1항에 있어서,The method of claim 1,
    바이러스가 용해된 상기 시약을 키트에 기설정된 시간 동안 반응시키는 단계 에서, 상기 키트와 바이러스가 용해된 상기 시약을 5~8분 동안 반응시키는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of reacting the virus-dissolved reagent in the kit for a predetermined time, the kit and the virus-reacting method in the air to react the reagent in which the virus is dissolved for 5-8 minutes.
  7. 제1항에 있어서,The method of claim 1,
    상기 아가플레이트를 기설정된 시간동안 배양기에 보관하는 단계에서, 상기 아가플레이트를 상기 배양기에 24시간동안 보관하는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of storing the agar plate in the incubator for a predetermined time, the virus and mold measurement method in the air to store the agar plate in the incubator for 24 hours.
  8. 포집 장치에 포집부를 결합하는 단계;Coupling the collecting unit to the collecting device;
    상기 포집 장치를 기설정된 시간 동안 작동시켜 공기 중 바이러스를 상기 포집부에 포집하는 단계;Operating the collecting device for a predetermined time to collect air virus in the collecting unit;
    상기 포집 장치와 상기 포집부를 분리하는 단계;Separating the collecting device and the collecting part;
    바이러스 또는 곰팡이 검지 단계를 선택하는 단계;Selecting a virus or mold detection step;
    상기 바이러스 또는 곰팡이 검지 단계를 선택하는 단계에서 바이러스 검지를 선택하는 경우에는,When the virus detection is selected in the step of selecting the virus or mold detection step,
    상기 포집부를 시약 0.5ml가 구비되어 있는 용기에 투입하고 10회 회전시켜, 상기 포집부에 포집된 바이러스를 상기 시약으로 용해하는 단계;Injecting the collecting unit into a container equipped with 0.5 ml of a reagent and rotating the sample 10 times to dissolve the virus collected in the collecting unit with the reagent;
    바이러스가 용해된 상기 시약을 키트에 다섯 방울 떨어트리고, 5~8분 동안 반응시키는 단계;5 drops of the reagent in which the virus is dissolved in the kit, and reacted for 5-8 minutes;
    상기 키트의 변화를 통해 바이러스의 유, 무를 판단하는 단계;Determining the presence or absence of a virus through a change in the kit;
    를 거쳐 바이러스를 측정하고,To measure the virus,
    상기 바이러스 또는 곰팡이 검지 단계를 선택하는 단계에서 곰팡이 검지를 선택하는 경우에는,When the mold detection is selected in the step of selecting the virus or mold detection step,
    상기 포집부를 곰팡이가 성장할 수 있는 아가플레이트에 문질러주는 단계;Rubbing the collection part on an agar plate on which mold can grow;
    상기 아가플레이트를 24시간동안 배양기에 보관하는 단계;Storing the agar plates in an incubator for 24 hours;
    상기 배양기에서 상기 아가플레이트를 꺼내 곰팡이를 측정하는 단계;Removing the agar plate from the incubator and measuring mold;
    를 거쳐 곰팡이를 측정하는 공기 중 바이러스 및 곰팡이 측정 방법.Methods of measuring viruses and fungi in the air to measure mold via
  9. 제8항에 있어서,The method of claim 8,
    상기 포집 장치로 공기를 포집하는 단계에서, 상기 포집 장치가 공기를 8-10lpm(liter per minute)의 유량으로 포집하는 공기 중 바이러스 및 곰팡이 측정 방법.In the step of collecting the air to the collecting device, the virus and mold measuring method in the air collecting the air at a flow rate of 8-10 lpm (liter per minute).
PCT/KR2019/002061 2018-05-09 2019-02-20 Method for measuring viruses and mold in air WO2019216533A1 (en)

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