WO2019152427A1 - Élimination des métaux lourds d'une protéine de riz - Google Patents

Élimination des métaux lourds d'une protéine de riz Download PDF

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Publication number
WO2019152427A1
WO2019152427A1 PCT/US2019/015703 US2019015703W WO2019152427A1 WO 2019152427 A1 WO2019152427 A1 WO 2019152427A1 US 2019015703 W US2019015703 W US 2019015703W WO 2019152427 A1 WO2019152427 A1 WO 2019152427A1
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WIPO (PCT)
Prior art keywords
water
rice protein
mixture
heavy metal
chelating agent
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PCT/US2019/015703
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English (en)
Inventor
Teodoro IANIRO
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Shaklee Corporation
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Publication date
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Publication of WO2019152427A1 publication Critical patent/WO2019152427A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/12—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
    • A23J1/125—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins

Definitions

  • This disclosure concerns the preparation of rice protein concentrate and/or methods for removing contaminants from rice protein material.
  • Rice is one of the most commonly consumed food crops. It is an abundant and inexpensive source for the development of various high-quality protein level products.
  • One such product is rice protein concentrate, which is an isolated vegetarian protein from rice, prepared by enzymatic digestion of comminuted rice grain.
  • Rice protein concentrate is a natural low-allergenic protein-containing substance derived from rice.
  • Rice protein concentrate that is produced from such grain typically contains at least a portion of the heavy metals that were present in the grain.
  • solubilized rice protein is formed, typically by the treatment of comminuted rice by enzymatic digestion and heating in an aqueous environment.
  • the solubilized rice protein is separated from insoluble materials and dried to produce the rice protein concentrate.
  • Rice protein concentrate precursor is combined with a chelating agent in an aqueous environment to provide a mixture of rice, water and chelating agent.
  • the mixture is maintained in a vessel at conditions sufficient for at least a portion of heavy metal contaminants, predominantly heavy metal ions, to be separated from the rice protein concentrate precursor and chelated. Thereafter, at least a portion of the water and the chelate are separated from the mixture, leaving rice protein concentrate that has a reduced content of heavy metals as compared to the solids in the protein concentrate precursor.
  • the chelating agent advantageously can be L-tartaric acid or carbohydrate-derived fulvic acid (CHD-FA).
  • the removal of heavy metals by processes described herein is simple to perform, uses only water and the chelating agent, and optionally can be performed in a single vessel that has temperature control, mixing and separation capabilities.
  • the content of lead (Pb) and other heavy metal can be reduced up to 95% or possibly more without using a hydrocarbon solvent.
  • the proteinaceous content of the rice protein concentrate after treatment is only slightly reduced, the resulting rice protein concentrate having greater than 90% of the protein content of the rice protein concentrate from which it is made.
  • FIG. 1 is a flow chart of a method for removing heavy metals from rice protein concentrate or isolate.
  • FIG. 2 is a flow chart of a method for removing heavy metals during an enzymatic hydrolysis process for the production of a rice protein concentrate or isolate from rice grain.
  • Brown rice As used herein, the terms“brown rice,”“unpolished rice,”“whole rice,” and“whole grain rice” refer to harvested rice grains from which the outer hull or husk has been removed. Specifically, brown rice as used herein means unpolished rice with only the husk removed.
  • White rice As used herein, the terms“white rice” and“polished rice” refer to rice grains formed by milling and/or polishing brown rice to remove the bran layer and germ.
  • Heavy metals As used herein, the terms“heavy metal” and“heavy metals” refer to arsenic, cadmium, lead, and/or mercury
  • Rice protein As used herein, the term“rice protein” refers to protein extracted from rice grain.
  • Rice protein material refers to a mixture of solids extracted from rice grain, a portion of which mixture is rice protein.
  • the rice protein material comprises at least 30 wt% rice protein of the total weight of the rice protein material.
  • the rice protein material comprises at least 40 wt% or at least 50 wt%, or at least 60 wt%, or at least 70 wt%, or at least 80 wt%.
  • Solubilized rice protein refers to a mixture of water and rice protein that is an intermediate obtained during production of rice protein concentrate by enzyme treatment of rice grain.
  • Rice protein concentrate refers to a dry composition containing rice protein, the composition having a rice protein content of at least 80% by weight of the total rice protein concentrate weight.
  • the term“rice protein concentrate,” as used herein is generic to the term“rice protein isolate.”
  • the rice protein concentrate may have a rice protein content of at least 85%, at least 90%, at least 95% or at least 98%, by weight.
  • Rice protein isolate refers to a dry composition containing rice protein, the composition having a rice protein level of at least 90%.
  • Rice protein concentrate precursor As used herein, the term“rice protein concentrate precursor” collectively refers to solubilized rice protein that contains heavy metal and dry rice protein material that contains heavy metal.
  • Chelating Agent is a chemical compound that coordinates with a metal to form a chelate.
  • a rice protein material that contains heavy metal is combined with a chelating agent in the presence of water to provide a mixture comprising rice protein, water and chelating agent. Best results are achieved when any added water is deionized water, but the water need not be deionized for successful operation.
  • the mixture is maintained in a vessel for a time sufficient for at least a portion of the heavy metal to be separated from the rice protein and chelated.
  • the mixture of rice protein material, water and chelating agent comprises, consists essentially of or consists of, from 13 wt % to 40 wt% of the total weight of the mixture rice protein material, 60 wt % to 87 wt% of the total weight of the mixture water, and 1 wt % to 10 wt% of the total weight of the mixture chelating agent.
  • the mixture of rice protein material, water and chelating agent comprises, consists essentially of or consists of from 6.5 wt % to 20 wt% of the total weight of the mixture rice protein material, 75 wt % to 87 wt% of the total weight of the mixture water, and 1 wt % to 5 wt% of the total weight of the mixture chelating agent.
  • the mixture of rice protein material, water and chelating agent comprises, consists essentially of or consists of from 3 wt % to 10 wt% of the total weight of the mixture rice protein material, 81 wt % to 87 wt% of the total weight of the mixture water, and 1 wt % to 2 wt% of the total weight of the mixture chelating agent.
  • “consists essentially of’ means at least 95% by weight of the total mixture are the recited components, or the result of interactions of those components. Or,“consists essentially of means the mixture contains only additional components that do not affect the treatment process in a manner that significantly effects the amount of separation after chelation (does not reduce the effectiveness of the separation by more than about 3%).
  • the mixture is formed by first adding the water to the rice protein material.
  • the chelating agent is next added, and the resulting water, rice protein material and chelating agent are mixed.
  • the mixing is performed by stirring vigorously, such as with a mechanical (e.g., a paddle stirrer) or a magnetic stirrer or any other suitable means of mixing.
  • excessive aeration of the mixture can be avoided by appropriate mixing speed and stirring-blade configuration.
  • the mixing speed is sufficient to fully agitate the solution.
  • the blade configuration if used for mixing, should provide a shearing effect.
  • the rice protein material can be agitated by another technique, such as shaking, but stirring is cost-effective and produces satisfactory results.
  • the chelating agent is not added until after the water and rice protein material are mixed. In some embodiments, the chelating agent is added a mixture of water and rice protein concentrate or water and rice protein isolate.
  • the mixture comprising, consisting of or consisting essentially of, the water, chelating agent and rice protein material, is maintained in a vessel for a time sufficient for at least a portion of the heavy metal to be separated from the rice protein and chelated. In certain embodiments, the mixture is maintained for
  • the water and the chelated heavy metal are separated from the treated mixture. Separation can be performed in part because the rice protein material has very poor to no solubility in water and the chelating agents are fully soluble in water. Physio-chemical interactions of the chelating agent and heavy metal binding sites on the proteins interact to allow for the removal of heavy metals, predominantly divalent in nature. Increased concentrations of the chelating agent itself will provide increased binding of the heavy metal component (per Le Chatelier’s Principle). In addition to varying the concentration of the chelating agent, other factors such at temperature, pressure, or contact time can be manipulated to shift the equilibrium to a higher state of heavy metal removal in the matrix.
  • the resulting separated rice protein has less than 50% or less than 40% or less than 30% or less than 20% or less than 10% or less than 5% or less than 2% or less than 1 % than was present in the rice protein material prior to treatment, or essentially no heavy metal remaining.
  • the steps of treatment with chelating agent and separation of chelated heavy metal may be conducted multiple times for each volume of input rice protein material to increase the removal of heavy metal. Diminishing amounts of heavy metal typically are removed by successive treatments.
  • the bulk of the heavy metals present in the rice protein material, from 40% to 90% of heavy metal removal can be removed after the first two treatment cycles, with higher amounts of removal with residence times of from 2 to 3 hours to 16 to 18 hours. Varying the concentration of the chelating agent used will vary the number of treatments needed to remove relatively higher heavy metal amounts.
  • the residence time and chelating agent concentration are preferably sufficiently high such that the bulk of heavy metal is removed after two treatments with the chelating agent, although three treatments may be beneficial in some instances.
  • the input rice protein material to be treated may be rice protein concentrate, rice protein isolate, or a dry rice protein material that has a lower concentration of rice protein than does rice protein concentrate.
  • the rice protein material to be treated should have at least 30 wt.% protein content.
  • the input rice protein material also can be solubilized rice protein that is an intermediate material that is formed during a process for enzymatic treatment of rice grain to extract rice protein.
  • the chelating agent may be tartaric acid, malic acid, citric acid, succinic acid, and/or calcium salts of such acids, namely calcium citrate, calcium malate, calcium succinate, calcium tartrate, or any mixture of two or more such acids and/or salts.
  • One or more sodium and/or potassium salts of tartaric acid, malic acid, citric acid, or succinic acid also could be used as the chelating agent.
  • Such a chelating agent is provided in an amount such that the weight of the chelating agent is 1% to 10% of the weight of the water in the mixture.
  • the chelating agent also can be selected from fulvic acids, humic acids, calcium salts of fulvic acids, calcium salts of humic acids, and mixtures thereof. Although calcium salts are highly effective, sodium and potassium salts of such acids also could be used. Monomeric fulvic acid and its calcium salt provide superior results as chelating agents as compared to dimer, trimer, oligomeric, or polymeric forms of fulvic acid and their respective calcium salt forms.
  • the chelating agent also can be humus material that contains a minimum of 30 wt.% fulvic acid, or can be a carbohydrate-derived fulvic acid material that contains from 30 wt % to 100 wt.% fulvic acid.
  • Fulvic acids are derived by removal of humic acids from humin by acidification or via a fermentation process from a carbohydrate-derived source.
  • Humic substances which are a group of compounds found in soils and water surfaces, are formed from the decomposition of organic matter. Generally divided into three components, based on their solubility in water as a function of pH, humic substances are referred to as humic acid, fulvic acid or humin.
  • Humic acids and fulvic acids represent the alkali soluble fragments and humic acids are commonly extracted using diluted alkali and precipitated with an acid to separate it from the soluble fulvic acids.
  • Such fulvic acid and humic acid chelating agents are effective when present at an amount such that the weight of the chelating agent is 0.1 % to 5% of the weight of the water in the mixture. In certain embodiments, the weight of the chelating agent is 0.5% to 5% of the weight of the water in the mixture. In certain embodiments, the weight of such a chelating agent is 0.5% to 1 % of the weight of the water.
  • chelating agents can be used to good effect in some embodiments of the disclosed processes.
  • Advantageous chelating agents are water soluble and/or are derived from a natural source.
  • the chelating agents are L-tartaric acid, carbohydrate-derived fulvic acid (CHD-FA), and humic acid that is enriched with at least 30% fulvic acid by weight.
  • the initial mixture comprising rice protein material, water and chelating agent comprises, consists essentially of or consists of 13 wt.% to 40 wt.% of a rice protein material and 60 wt.% to 87 wt.% water.
  • Chelating agent is provided in an amount that the weight of the chelating agent is 0.1 % to 10% of the weight of the water in the mixture.
  • the protein content of the treated rice protein product will depend on the nature of the inputted rice protein material.
  • the final protein level can be as low as 30% in some cases.
  • the protein content typically is at least 80% of the rice protein product weight when the inputted rice protein material for treatment is rice protein concentrate or rice protein isolate.
  • FIG. 1 illustrates an embodiment of the disclosed processes.
  • the source of rice protein material to be treated is rice protein concentrate powder or rice protein isolate powder that contain heavy metal.
  • the rice protein concentrate or isolate powder (i.e., the rice protein material) and deionized water are combined at a liquid to solid range ratio of > 2.5:1 and ⁇ 7.5:1 on a weight to weight basis in an appropriately sized vessel that has mixing and temperature control capabilities. Operation is possible with a higher amount of water, if desirable.
  • the ratio is preferably such that the rice protein concentrate or isolate is submerged and easy to agitate.
  • a liquid to solid ratio of up to 10:1 may be used, or a ratio reduced down to as low as 2:1 may be used, if desirable, depending upon the protein level of the rice protein concentrate or isolate.
  • the use of excess water could affect the nutrient profile of the resulting rice protein concentrate or isolate, but should not adversely affect chelation of the heavy metal.
  • the use of excess water could also increase processing time and processing costs in a commercial scale operation.
  • 2.5 parts deionized water are combined with one part rice protein concentrate or isolate on a weight to weight basis.
  • the deionized water is added to the vessel and chilled, if necessary, to be in a temperature in the range of 5-25°C. While stirring the chilled water, the rice protein concentrate or isolate is added to the water in the vessel to form a mixture of rice protein concentrate and water. Agitation is continued after the addition of the rice protein concentrate or isolate. In certain embodiments, the temperature of the mixture is maintained at no more than 25°C after addition of the rice protein concentrate or isolate.
  • the process can be performed at a temperature somewhat above 25°C, in particular at a temperature of up to 35°C.
  • Operation at too high a temperature could cause excessive protein loss, which is not desired.
  • Operation at a temperature of as low as 5°C is possible and may be advantageous on a production scale.
  • a temperature range of 5-25°C thus is operable, particular temperature ranges of 5-8°C and 20-25°C being appropriate for commercial production in some instances.
  • the mixture of rice protein concentrate or isolate and water can be agitated by stirring vigorously. Excessive aeration of the mixture can be avoided by appropriate mixing speed and stirring-blade configuration.
  • the rice protein concentrate or isolate can be agitated by any other suitable mixing technique.
  • the chelating agent then is added to the mixture of water and rice protein concentrate or isolate.
  • the chelating agent is added in an amount such that the weight of the chelating agent is 0.1 % to 10% of the weight of the water.
  • the chelating agent is added in an amount such the weight of the chelating agent is at least 0.1% of the weight of the water.
  • L-tartaric acid is added as the chelating agent to the mixture of water and rice in an amount such that the weight of the L-tartaric acid is 5% of the weight of the water.
  • the L-tartaric acid is added as a powder to the rice protein water mixture itself while continuously mixing.
  • the L-tartaric acid should be of sufficient purity for use in the treatment of food, as known to those of ordinary skill in the art.
  • the mixture of water, rice and L-tartaric acid is mixed, for example, a minimum of 4 hours but not more than 24 Hours. Operating under these conditions results in chelation of heavy metal ions by the L-tartaric acid.
  • water and chelated heavy metal are separated from the mixture by, e.g., centrifugation.
  • the mixture of water, chelated heavy metal, rice protein material and residual L-tartaric acid is transferred from the vessel to a centrifuge device.
  • a decanter centrifuge or a disc centrifuge is used to fully separate rice protein solids from supernatant. Rice protein solids retained in the centrifuge have a reduced content of heavy metals as compared to the content of heavy metals in the rice protein concentrate or isolate before the chelation treatment.
  • the rice protein solids retained in the centrifuge advantageously are further purified by a rinsing process in which sufficient deionized water is added to fully cover the rice protein solids.
  • the mixture of rice protein solids and water then is briefly agitated. Agitation for 30-60 minutes can be helpful to remove residual chelating agent and chelate that may be present. Water, possibly along with some residual chelating agent and chelate, then is removed from the mixture by centrifugation.
  • This rinsing process may be performed plural times. In certain embodiments, the rinsing process may be performed at least three times. Conducting the rinsing process three times has been shown to be sufficient to remove substantially all remaining chelating agent and chelate.
  • the mixture of rice protein and water preferably does not exceed 25°C to avoid undue removal of proteinaceous material from the rice protein, and best should not exceed 8°C, if possible.
  • the result of the process is a rice protein material having a protein content that typically is at least 80% when the inputted rice protein material for the treatment process is rice protein concentrate or rice protein isolate.
  • the process of treating rice protein concentrate or isolate as described above enables the production of a rice protein material having a greatly reduced content of heavy metal, especially lead (Pb), as compared to the content of heavy metal in the pre-treated rice protein concentrate or isolate.
  • the vessel may have a heat exchanger in the form of jacket that define a chamber between the jacket and the exterior of the vessel wall, through which chamber glycol at a desired temperature is circulated. Temperature of liquid inside the vessel is maintained at 20-25°C by heat exchange with the glycol.
  • the amount of L-tartaric acid is 5% of the amount of the water on a weight to weight basis. Stirring is continued until the L-tartaric acid is fully dissolved.
  • the required mixing time should not exceed 30 minutes.
  • the aqueous solution of L-tartaric acid and suspended rice protein material then is pumped through a decanter centrifuge to separate liquid from solids. The supernatant solution is discarded in an environmentally appropriate manner.
  • the resulting rice protein material which has a reduced content of heavy metal, is transferred into a 200 gallon vessel having mixing and siphoning capabilities.
  • Dl water may be used as an aid in transferring the rice protein material where applicable.
  • Dl water is added to the 200 gallon vessel in an amount sufficient to bring the total water content of the vessel to 130-150 gallons.
  • the total content of Dl water should not exceed 150 gallons.
  • the resulting suspension is stirred for 15 minutes.
  • the suspension is then allowed to sit for 30 minutes to 1 hour to allow protein solids to settle. After the settling period, the supernatant is siphoned off and discard.
  • the resulting rice protein material is transferred to an appropriately sized container, with the aid of additional Dl water if needed to accomplish the material transfer.
  • the rice protein material then is spray dried to produce rice protein concentrate that has a lower heavy metal content than the originally input rice protein material.
  • the vessel may have a heat exchanger in the form of jacket that define a chamber between the jacket and the exterior of the vessel wall, through which chamber glycol at a desired temperature is circulated. Temperature of liquid inside the vessel is maintained at 20-25°C by heat exchange with the glycol.
  • fulvic acid powder While mixing, 12.5 pounds of 30% fulvic acid powder are blended into the water.
  • the amount of fulvic acid powder is 1 % of the amount of the water on a weight to weight basis. Stirring is continued until the fulvic acid powder if fully dissolved.
  • the required mixing time should not exceed 30 minutes.
  • aqueous solution of fulvic acid and suspended rice protein material then is pumped through a decanter centrifuge to separate liquid from solids. The supernatant solution is discarded in an
  • the resulting rice protein material which has a reduced content of heavy metal, is transferred into a 75 gallon vessel having mixing and siphoning capabilities.
  • Dl water may be used as an aid in transferring the rice protein material where applicable.
  • Dl water is added to the 75 gallon vessel in an amount sufficient to bring the total water content of the vessel to 45-55 gallons.
  • the total content of Dl water should not exceed 55 gallons.
  • the resulting suspension is stirred for 15 minutes.
  • the suspension is then allowed to sit for 30 minutes to 1 hour to allow protein solids to settle. After the settling period, the supernatant is siphoned off and discard.
  • the resulting rice protein material is transferred to an appropriately sized container, with the aid of additional Dl water if needed to accomplish the material transfer.
  • the rice protein material then is spray dried to produce rice protein concentrate that has a lower heavy metal content than the originally input rice protein material.
  • the inputted rice protein material could be dry rice protein material that has a lower protein content than rice protein concentrate or isolate.
  • a comparable process can be used to remove heavy metal from a rice protein material that already is in an aqueous environment.
  • a comparable process can be used to remove heavy metal from rice protein that is an intermediate formed during an enzymatic hydrolysis process for the production of rice protein concentrate from rice grain.
  • An example of such as process for removing heavy metal from solubilized rice protein is shown in FIG. 2.
  • the only factors to be controlled relate to addition of a chelating agent, in particular, the amount and selection of the chelating agent, the timing of the addition of the chelating agent, and environmental conditions inside the vessel which contains the solubilized rice protein during removal of the heavy metal.
  • the most important operational criteria are mixing time, water temperature, liquid-to-solid ratios, and ensuring that the rice protein avoids excessive aeration in the suspension by appropriate mixing speed and stirring-blade configuration during treatment with the chelating agent. Additionally, if treatment of rice protein material is being performed during an enzymatic hydrolysis step or shortly thereafter, an appropriate blending speed and blade needs to be in place to avoid damage to the rice protein material.
  • the chelating agent can be added to the reaction mixture during or after an enzymatic hydrolysis step of such a process, at a step where rice protein is in an aqueous environment.
  • the chelating agent could be added to the reaction mixture during a washing, cleaning, or separation step that involves the use of water. In the process of FIG.
  • the chelating agent could be added at the step labeled“Water Based Solution pH and Concentration Adjustments.” It also would be possible to add the chelating agent at a step earlier in the process, such as the step labeled“Soaking and Cleaning” or the step labeled“Enzymatic Hydrolysis.” In certain embodiments, the chelating agent would be added in an amount such that the weight of the chelating agent is from 0.1 % to 10% of the weight of the water in the suspension of rice protein to be purified. The treatment time would be similar to that of the process for the treatment of dry rice protein concentrate or rice protein isolate. The apparatus used for an existing enzymatic hydrolysis process may be altered to better remove chelated heavy metal and residual chelating agent prior to the final drying step.
  • a flow of rice protein concentrate precursor can be processed continuously or in periodic pulses using the same process parameters.
  • an apparatus for processing a flow of rice protein concentrate precursor could comprise an extractor vessel (having chilling, mixing, and draining capabilities), a conveyor for continuously or periodically loading protein concentrate precursor into the extractor vessel, a holding tank (having chilling and mixing capabilities) to hold a mixture of Dl water and chelating agent, a holding tank (having chilling capabilities) containing Dl water only, a separator unit for separating solids from liquid, a dryer, and various transfer pumps for moving fluids between vessels.
  • a sequence of batch extractor vessels as many as twelve in a row, can be provided where each vessel has a tank of about 2,000 gallons overall volume capacity. Each tank would have mixing capabilities and a glycol chilling system to control temperature inside the tank. After rice protein concentrate precursor is added into the individual extractors via a conveyor, a mixture of chilled water and chelating agent is pumped into each extractor vessel for mixing. After the mixing is completed, each extractor would be drained to remove as much water as possible. Dl water then would be pumped into each tank to briefly rinse the retained rice protein solids, followed by draining once again to remove the bulk of the left over chelate and chelating agent.
  • a continuous or periodic process could also be performed using a single extraction vessel having one or more component inlets that are spaced apart from one or more component outlets, with a passageway extending between an inlet region adjacent to the inlets and an outlet region adjacent to the outlets.
  • rice protein concentrate precursor would be continuously or periodically added into the inlet region of the passageway via a conveyor.
  • Chilled water, if needed, and chelating agent also would be continuously or periodically conveyed into the inlet region of the passageway of the extractor vessel for mixing with the protein concentrate precursor. The mixture of protein concentrate precursor, water and chelating agent then would flow through the passageway toward the outlet region.
  • the mixture While flowing from the inlet region to the outlet region, the mixture would be stirred by mixing blades or the passageway could be defined by a cylindrical rotary tank that would rotate and thereby tumble the mixture as it moves from the inlet region to the outlet region.
  • Rinsing stations could be provided at spaced apart locations along the passageway to drain water and chelated heavy metal from the passageway and to add fresh water for rinsing.

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Abstract

La présente invention concerne un précurseur de concentré de protéine de riz qui est combiné à un agent chélatant dans un environnement aqueux pour fournir un mélange de protéine de riz, d'eau et d'agent chélatant. Dans certains modes de réalisation, le mélange est maintenu dans un récipient dans des conditions suffisantes pour qu'au moins une partie de tout contaminant de type métal lourd soit séparée du précurseur de concentré de protéine de riz et chélatée. Le chélate de métal lourd résultant est séparé de la protéine de riz pour fournir un concentré de protéine de riz ayant une teneur réduite en métaux lourds.
PCT/US2019/015703 2018-01-31 2019-01-29 Élimination des métaux lourds d'une protéine de riz WO2019152427A1 (fr)

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CN105394520A (zh) * 2015-10-30 2016-03-16 江南大学 一种整粒大米除镉的方法

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Publication number Priority date Publication date Assignee Title
CN103283932A (zh) * 2013-04-22 2013-09-11 义乌市海之纳生物工程有限公司 一种脱镉大米蛋白及其制备方法和应用
CN103549234A (zh) * 2013-10-23 2014-02-05 华中农业大学 一种消减谷物重金属的方法
CN104957448A (zh) * 2015-07-29 2015-10-07 湖南农业大学 一种酸溶联用发酵脱除大米中镉的方法
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