WO2019103442A3 - CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도 - Google Patents
CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도 Download PDFInfo
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- WO2019103442A3 WO2019103442A3 PCT/KR2018/014312 KR2018014312W WO2019103442A3 WO 2019103442 A3 WO2019103442 A3 WO 2019103442A3 KR 2018014312 W KR2018014312 W KR 2018014312W WO 2019103442 A3 WO2019103442 A3 WO 2019103442A3
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- genome editing
- crispr
- cpf1 system
- editing composition
- cpf1
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N9/22—Ribonucleases RNAses, DNAses
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
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- C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects
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Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201880085616.XA CN111836894B (zh) | 2017-11-21 | 2018-11-21 | 用于使用CRISPR/Cpf1***进行基因组编辑的组合物及其用途 |
AU2018370588A AU2018370588B2 (en) | 2017-11-21 | 2018-11-21 | Composition for Genome Editing Using Crispr/CPF1 System and Use Thereof |
EP18882084.9A EP3715461A4 (en) | 2017-11-21 | 2018-11-21 | GENOMIC EDITION COMPOSITION USING A CRISPR / CPF1 SYSTEM AND ITS USE |
US16/765,553 US11667917B2 (en) | 2017-11-21 | 2018-11-21 | Composition for genome editing using CRISPR/CPF1 system and use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20170155927 | 2017-11-21 | ||
KR10-2017-0155927 | 2017-11-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2019103442A2 WO2019103442A2 (ko) | 2019-05-31 |
WO2019103442A3 true WO2019103442A3 (ko) | 2019-07-18 |
Family
ID=66630759
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2018/014312 WO2019103442A2 (ko) | 2017-11-21 | 2018-11-21 | CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도 |
Country Status (6)
Country | Link |
---|---|
US (1) | US11667917B2 (ko) |
EP (1) | EP3715461A4 (ko) |
KR (1) | KR102168489B1 (ko) |
CN (1) | CN111836894B (ko) |
AU (1) | AU2018370588B2 (ko) |
WO (1) | WO2019103442A2 (ko) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
CN114846146B (zh) * | 2019-10-29 | 2024-04-12 | 基恩科雷有限责任公司 | 用于增加CRISPR/Cas12f1***的效率的工程化引导RNA及其用途 |
WO2021092519A1 (en) * | 2019-11-08 | 2021-05-14 | University Of Florida Research Foundation | Crispr complex-based detection system and method |
CN111235130B (zh) * | 2019-11-15 | 2022-11-25 | 武汉大学 | II类V型CRISPR蛋白CeCas12a及其在基因编辑的应用 |
KR102551876B1 (ko) * | 2019-12-18 | 2023-07-05 | 한국생명공학연구원 | Cpf1 및 키메릭 DNA-RNA 가이드를 포함하는 유전체 교정 또는 발현 억제용 조성물 |
KR102493512B1 (ko) * | 2020-10-08 | 2023-02-01 | 주식회사 진코어 | CRISPR/Cas12a 시스템을 위한 엔지니어링 된 crRNA |
CN116568803A (zh) * | 2020-10-08 | 2023-08-08 | 基恩科雷有限责任公司 | 用于增加CRISPR/Cas12f1***的效率的包含富含U的尾部的经工程化的引导RNA及其用途 |
CA3220294A1 (en) * | 2021-05-14 | 2022-11-17 | Genkore Inc. | Composition and method for treatment of lca10 using rna-guided nuclease |
CN113355362B (zh) * | 2021-06-16 | 2022-06-07 | 北京大学 | 化学修饰CRISPR/Cpf1复合物的用途 |
KR20230121569A (ko) * | 2022-02-09 | 2023-08-18 | 주식회사 진코어 | 상동지정복구를 위한 TaRGET 시스템 및 이를 이용한 유전자 편집 방법 |
WO2023172116A1 (ko) * | 2022-03-10 | 2023-09-14 | 주식회사 진코어 | 듀센 근이영양증 치료를 위한 유전자 편집 시스템 및 이를 이용한 질병 치료 방법 |
KR20230142365A (ko) * | 2022-03-30 | 2023-10-11 | 주식회사 진코어 | 어셔 증후군 치료를 위한 유전자 편집 시스템 |
CN116751763B (zh) * | 2023-05-08 | 2024-02-13 | 珠海舒桐医疗科技有限公司 | 一种Cpf1蛋白、V型基因编辑***及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20170063399A (ko) * | 2015-11-30 | 2017-06-08 | 기초과학연구원 | F. novicida 유래 Cas9을 포함하는 유전체 교정용 조성물 |
KR20170068400A (ko) * | 2015-12-08 | 2017-06-19 | 기초과학연구원 | Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102182847B1 (ko) | 2012-10-23 | 2020-11-27 | 주식회사 툴젠 | 표적 DNA에 특이적인 가이드 RNA 및 Cas 단백질을 암호화하는 핵산 또는 Cas 단백질을 포함하는, 표적 DNA를 절단하기 위한 조성물 및 이의 용도 |
CN106244591A (zh) * | 2016-08-23 | 2016-12-21 | 苏州吉玛基因股份有限公司 | 修饰crRNA在CRISPR/Cpf1基因编辑***中的应用 |
CN108130342B (zh) * | 2016-12-01 | 2022-01-11 | 中国科学院分子植物科学卓越创新中心 | 基于Cpf1的植物基因组定点编辑方法 |
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2018
- 2018-11-21 AU AU2018370588A patent/AU2018370588B2/en active Active
- 2018-11-21 KR KR1020180144185A patent/KR102168489B1/ko active IP Right Grant
- 2018-11-21 EP EP18882084.9A patent/EP3715461A4/en active Pending
- 2018-11-21 US US16/765,553 patent/US11667917B2/en active Active
- 2018-11-21 WO PCT/KR2018/014312 patent/WO2019103442A2/ko unknown
- 2018-11-21 CN CN201880085616.XA patent/CN111836894B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20170063399A (ko) * | 2015-11-30 | 2017-06-08 | 기초과학연구원 | F. novicida 유래 Cas9을 포함하는 유전체 교정용 조성물 |
KR20170068400A (ko) * | 2015-12-08 | 2017-06-19 | 기초과학연구원 | Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도 |
Non-Patent Citations (3)
Title |
---|
EAST-SELETSKY, ALEXANDRA ET AL.: "RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes", MOLECULAR CELL, vol. 66, no. e1-e3, 4 May 2017 (2017-05-04), pages 373, XP029999996 * |
LI, BIN ET AL.: "Engineering CRISPR-Cpfl crRNAs and aRNAs to maximize genome editing efficiency", NAT BIOMEDENG., vol. 1, no. 5, May 2017 (2017-05-01), pages 1 - 21, XP055564263 * |
MCMANUS, MICHAEL T. ET AL.: "Trypanosoma brucei Guide RNA Poly(U) Tail Formation Is Stabilized by Cognate mRNA", MOL. CELL . BIOL., vol. 20, no. 3, 2000, pages 883 - 891, XP055625013 * |
Also Published As
Publication number | Publication date |
---|---|
WO2019103442A2 (ko) | 2019-05-31 |
KR102168489B1 (ko) | 2020-10-22 |
EP3715461A4 (en) | 2021-09-08 |
CN111836894A (zh) | 2020-10-27 |
AU2018370588A1 (en) | 2020-07-09 |
EP3715461A2 (en) | 2020-09-30 |
US20200308583A1 (en) | 2020-10-01 |
KR20190058358A (ko) | 2019-05-29 |
AU2018370588B2 (en) | 2022-09-08 |
US11667917B2 (en) | 2023-06-06 |
CN111836894B (zh) | 2023-11-10 |
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