WO2019071328A1 - Amélioration apportée à un procédé d'hydrolyse d'amidon dans l'industrie de l'alcool - Google Patents

Amélioration apportée à un procédé d'hydrolyse d'amidon dans l'industrie de l'alcool Download PDF

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Publication number
WO2019071328A1
WO2019071328A1 PCT/BR2018/000063 BR2018000063W WO2019071328A1 WO 2019071328 A1 WO2019071328 A1 WO 2019071328A1 BR 2018000063 W BR2018000063 W BR 2018000063W WO 2019071328 A1 WO2019071328 A1 WO 2019071328A1
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WO
WIPO (PCT)
Prior art keywords
hydrolysis
starch
energy
improvement
industry
Prior art date
Application number
PCT/BR2018/000063
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English (en)
Portuguese (pt)
Inventor
Leonardo GARNICA
Antonio Pedro LOURENÇO
Original Assignee
Garnica Leonardo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Garnica Leonardo filed Critical Garnica Leonardo
Publication of WO2019071328A1 publication Critical patent/WO2019071328A1/fr

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Definitions

  • the present report refers to an invention for improvement in the process of hydrolysis of starch in the alcohol industry and more specifically to improvements applied in the stages of the production process of alcohol from starches as in the case of maize, rice and wheat grains and starchy, such as cassava and potato.
  • the improvement is implemented in the production stage of the must, or saccharification, with the hydrolysis of polysaccharides, in a separate process or carried out simultaneously with the fermentation of sugars produced in saccharification.
  • the simultaneous process has as objective, besides reducing the processing time, impacting the productivity of the process, it allows the minimization of sugar losses from the saccharification, in the separation of the hydrolysis products.
  • Simultaneous fermentation that is, the application of fermentative microorganism to the system in enzymatic reaction, involving the joint operations of saccharification with fermentation, is a fact present in the alcoholic production from corn.
  • biofuels stand out as components of the world energy matrix, supported by environmental impacts.
  • Ethanol follows its long history as a fuel and has been largely produced by the fermentation of sugars directly fermentable as sucrose, glucose and fructose. These sugars are easily obtained from crops such as sugar cane, beet, fruits, etc.
  • the amylose and amylopectin polysaccharides of malt are converted into smaller sugars, maltose and glucose, under the action of enzymes, such as a- and ⁇ -amylase, cellulase and ⁇ -glycosidase, according to the appropriate schedule thermo-mass, in the maintenance of the process, typical for the saccharification process.
  • enzymes such as a- and ⁇ -amylase, cellulase and ⁇ -glycosidase
  • thermo-mass thermo-mass
  • the starch can be hydrolysed thermochemically or by enzymes.
  • the chemical hydrolysis requires the use of special reactors, of concentrated acid at high temperatures and, consequently, of high cost. Enzymatic hydrolysis is more time-consuming, but is conducted in conditions. This makes the enzymatic hydrolysis process more attractive in the bioenergy industry.
  • the treatment with capacitive energy field, during the enzymatic hydrolysis of the starch, is the technical approach to increase the conversion of starch to sugars.
  • this application can be made in an isolated or simultaneous process of saccharification and fermentation.
  • the processing refers to the controlled application of an electric field or the modulation of capacitive energy supplied to the reacting system, in order to improve the biochemical and enzymatic processes.
  • Moderate electrical fields can improve mass transfer by increasing the mobility of charged molecules through the application of electrostatic forces.
  • capacitive energy treatments to improve enzymatic hydrolysis is an innovative approach that can alleviate cost and revenue ratios and stimulate the implementation of starch production technologies.
  • capacitive energy systems are applied, specially designed and designed for the operational conditions understood by the process. Acting as accelerators and / or attenuators of the object process, these energy accumulators act on the chemical and biochemical system, giving additional energy to the manifestation of the physical-chemical transformations of the mixture in process.
  • These applications can be made in batch, semi-continuous and continuous production regime processes, using the amylase enzyme complex or different types of enzymes and their possible combinations, said cocktails.
  • the present improved process is novel and seeks the mass and kinetic maximization of the hydrolytic conversion of the starch, employing capacitive energy in the catalytic function of the enzymatic activity in conjunction with behavior induced to the amylaceous structure.
  • the present invention extends to the combined action of the capacitive energy, to the simultaneous reactions that occur in the hydrolysis of the starch and the fermentation of the fermentable sugars.
  • the present invention relates to improvement in the hydrolysis process of starch in the alcohol industry, which brings about an increase in production and productivity in the ethanol production process from corn.
  • This improvement is applied in the step of hydrolysis of the starch, both in an isolated process of saccharification and simultaneously with the fermentation, using capacitive energy in the catalytic function of the enzymatic activity in conjunction with the behavior induced to the structures of the amylaceous parts.
  • This capacitive energy acts as an accelerator and / or attenuator of the wort production process, object of the present invention, being that these energetic capacitors act on the chemical and biochemical system, giving additional energy to the manifestation of the physicochemical and biochemical transformations of the mixture in process.
  • energy capacitors also understood as energy converters
  • energy converters must necessarily have differentiated operational and constructive conditions, in relation to the functional objectives and characteristics of the mixture that qualify the medium.
  • such converters are capable of delivering to the medium in reactions the capacitive energy per cubic meter of the converter from 20J / hr 3 to 1500KJ / hr 3 and more typically from 2.5KJ / hr 3 to 1 .
  • OOOKJ / hm 3 energy that is transferred to redox reactions at this stage of the production process.
  • the present invention is comprised of improvement in the process of producing ethanol from the starch with benefits in maximizing the yield of sugars desired in the wort and reduction in the preparation time of the same.
  • the capacit ⁇ va energy, delivered to the system in reaction acts catalytically in the hydrolysis of the polysaccharides, maximizing the formation of fermentable and non fermentable sugars, reducing the hydrolysis time with the consequent reduction of saccharification time, in the process alone or simultaneously with fermentation , impacting the productivity of this phase.
  • the reduction of this time is a function of the characteristics involved in the qualities and properties desired for the final wort that will be brought to fermentation, or processed in the simultaneous fermentation.
  • a preferred embodiment of the present invention has as novel and novel element in this process of ethanol production from starch the use of capacitive energy converters which act in the step of hydrolysis, said energy converter being said capacitive units, or capacitors, which are immersed inside the reactors or vessels where the enzymatic hydrolysis of the starch occurs, or saccharification. Process of saccharification alone or simultaneously with the fermentation of fermentable sugars from saccharification.
  • capacitors are made of specially designed armatures and constructed with materials and geometries suitable for the manufacturing stage and the characteristics of the vessels of the production process, such as shape, volume, internal diameter and height.
  • the materials used in the construction of the reinforcement can be, for example, austenitic stainless steel or titanium (Ti) or other conductive metals, with application and finishing pertinent to the application.
  • the capacitors of the armor may i nda be coated with electrically insulating material or not.
  • control and regulation of the capacitive energy employed in this stage of the process has a significant importance in the control of the production process, since it is this control that determines some characteristics of the product to be obtained (fermentation must).
  • This control aims at the maximization of capacitive energy transferred to the system in process, thus guaranteeing the effectiveness of the productive stage.
  • Sensors of pH, temperature, dissolved oxygen, soluble solids content, electrical conductivity, voltage and current are used and act directly on the energy converters.
  • the processing of the data obtained by these sensors acts on the capacitive energy transferred to the converter, by means of automatic adjustment of the electric voltage levels applied to the reinforcement, concomitantly with adjustments in the capacitive characteristics of the same.

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé amélioré d'hydrolyse enzymatique de l'amidon dans l'industrie de l'alcool, augmentant le rendement de la production de sucre converti ainsi que la vitesse de la réaction d'hydrolyse. Pendant l'hydrolyse enzymatique de l'amidon, le mélange en réaction est soumis à au moins une intervention d'énergie capacitive, de manière continue ou intermittente, cette énergie étant fournie au système en réaction.
PCT/BR2018/000063 2017-10-11 2018-10-09 Amélioration apportée à un procédé d'hydrolyse d'amidon dans l'industrie de l'alcool WO2019071328A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BR1020170220370 2017-10-11
BR102017022037-0A BR102017022037A2 (pt) 2017-10-11 2017-10-11 Melhoria em processo de hidrólise do amido na indústria alcooleira

Publications (1)

Publication Number Publication Date
WO2019071328A1 true WO2019071328A1 (fr) 2019-04-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2018/000063 WO2019071328A1 (fr) 2017-10-11 2018-10-09 Amélioration apportée à un procédé d'hydrolyse d'amidon dans l'industrie de l'alcool

Country Status (2)

Country Link
BR (1) BR102017022037A2 (fr)
WO (1) WO2019071328A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0041373A1 (fr) * 1980-05-30 1981-12-09 Ppg Industries, Inc. Electrostimulation de réactions microbiennes
US20080311639A1 (en) * 2007-06-15 2008-12-18 Tajchai Navapanich Pulsed electric field apparatus and methods for ethanol production
US20130288307A1 (en) * 2008-04-30 2013-10-31 Xyleco, Inc. Processing Biomass Containing Materials
WO2014100572A1 (fr) * 2012-12-21 2014-06-26 Colorado Energy Research Technologies, LLC Systèmes et procédés d'amélioration de fermentation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0041373A1 (fr) * 1980-05-30 1981-12-09 Ppg Industries, Inc. Electrostimulation de réactions microbiennes
US20080311639A1 (en) * 2007-06-15 2008-12-18 Tajchai Navapanich Pulsed electric field apparatus and methods for ethanol production
US20130288307A1 (en) * 2008-04-30 2013-10-31 Xyleco, Inc. Processing Biomass Containing Materials
WO2014100572A1 (fr) * 2012-12-21 2014-06-26 Colorado Energy Research Technologies, LLC Systèmes et procédés d'amélioration de fermentation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DURHAM, E. K.: "Moderate electric field treatment for saccharification of cellulosic materials", DISSERTATION, 2015, Ohio, pages 1 - 121, XP055591957 *
JEON, B. Y. ET AL.: "Production of ethanol directly from potato starch by mixed culture of Saccharomyces cerevisiae and Aspergillus niger using electrochemical bioreactor", J MICROBIOL BIOTECHNOL., vol. 18, no. 3, 1 March 2008 (2008-03-01), pages 545 - 551, XP002712838, ISSN: 1017-7825 *
KUMAR, P. ET AL.: "Pulsed Electric Field Pretreatment of Switchgrass and Wood Chip Species for Biofuel Production", INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, vol. 50, no. 19, 30 August 2011 (2011-08-30), pages 10996 - 11001, XP055591966, ISSN: 0888-5885, DOI: 10.1021/ie200555u *
VEGA-MERCADO, H. ET AL.: "Pulsed electric fields in food preservation", HANDBOOK OF FOOD PRESERVATION, 18 May 2007 (2007-05-18), XP055591951, ISSN: 0924-2244 *
ZHOU, Y. ET AL.: "Electrofluid enhanced hydrolysis of maize starch and its impacts on physical properties", RSC ADV., vol. 7, no. 31, 31 March 2017 (2017-03-31), pages 19145 - 19152, XP055591955, ISSN: 2046-2069, DOI: 10.1039/C7RA00080D *

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