WO2019022542A3 - Method for isolating extracellular vesicles using cations - Google Patents

Method for isolating extracellular vesicles using cations Download PDF

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Publication number
WO2019022542A3
WO2019022542A3 PCT/KR2018/008485 KR2018008485W WO2019022542A3 WO 2019022542 A3 WO2019022542 A3 WO 2019022542A3 KR 2018008485 W KR2018008485 W KR 2018008485W WO 2019022542 A3 WO2019022542 A3 WO 2019022542A3
Authority
WO
WIPO (PCT)
Prior art keywords
extracellular vesicles
isolating
cations
present
isolating extracellular
Prior art date
Application number
PCT/KR2018/008485
Other languages
French (fr)
Korean (ko)
Other versions
WO2019022542A2 (en
Inventor
고용송
이창진
김지현
송성현
Original Assignee
㈜로제타엑소좀
포항공과대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ㈜로제타엑소좀, 포항공과대학교 산학협력단 filed Critical ㈜로제타엑소좀
Priority to CN201880062590.7A priority Critical patent/CN111148828A/en
Priority to JP2020527719A priority patent/JP2020528766A/en
Priority to US16/634,086 priority patent/US11904259B2/en
Priority to EP18837656.0A priority patent/EP3660142A4/en
Priority claimed from KR1020180087354A external-priority patent/KR102107844B1/en
Publication of WO2019022542A2 publication Critical patent/WO2019022542A2/en
Publication of WO2019022542A3 publication Critical patent/WO2019022542A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a method for isolating extracellular vesicles using cations, and more particularly, to a method for isolating extracellular vesicles from various samples by using the affinity between the extracellular vesicles and cations. A method for isolating extracellular vesicles according to the present invention does not require expensive equipment, can be applied irrespective of sample amount, and has the advantage of being capable of efficiently isolating the extracellular vesicles while preserving the shape or characteristics thereof. Moreover, the method according to the present invention can be combined with existing isolation methods to maximize extracellular vesicle isolation efficiency, and can be applied to disease diagnosis, disease treatment, and multi-omics research using isolated extracellular vesicles, as well as to research on the properties of extracellular vesicles.
PCT/KR2018/008485 2017-07-26 2018-07-26 Method for isolating extracellular vesicles using cations WO2019022542A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN201880062590.7A CN111148828A (en) 2017-07-26 2018-07-26 Method for separating extracellular vesicles using cations
JP2020527719A JP2020528766A (en) 2017-07-26 2018-07-26 Isolation method of extracellular endoplasmic reticulum using cations
US16/634,086 US11904259B2 (en) 2017-07-26 2018-07-26 Method for isolating extracellular vesicles using cations
EP18837656.0A EP3660142A4 (en) 2017-07-26 2018-07-26 Method for isolating extracellular vesicles using cations

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20170094888 2017-07-26
KR10-2017-0094888 2017-07-26
KR1020180087354A KR102107844B1 (en) 2017-07-26 2018-07-26 Method of isolating extracellular vesicles using cation
KR10-2018-0087354 2018-07-26

Publications (2)

Publication Number Publication Date
WO2019022542A2 WO2019022542A2 (en) 2019-01-31
WO2019022542A3 true WO2019022542A3 (en) 2019-03-28

Family

ID=65040760

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/008485 WO2019022542A2 (en) 2017-07-26 2018-07-26 Method for isolating extracellular vesicles using cations

Country Status (1)

Country Link
WO (1) WO2019022542A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113748217B (en) 2019-03-01 2023-08-25 仁慈生物分析公司 Systems, compositions, and methods for target entity detection

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120023684A (en) * 2009-04-23 2012-03-13 소시에다지 베네피시엔찌 이스라엘리따 브라질레이라 오스삐따우 알버트 아인슈타인 Method for isolating exosomes from biological solutions using iron oxide nanoparticles
WO2015048566A1 (en) * 2013-09-26 2015-04-02 The General Hospital Corporation Methods of isolating extracellular vesicles
WO2016033695A1 (en) * 2014-09-05 2016-03-10 Exerkine Corporation Exosome isolation
KR20160116802A (en) * 2015-03-31 2016-10-10 포항공과대학교 산학협력단 Isolation Method of Extracellular Vesicles by Aqueous Two-phase System

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120023684A (en) * 2009-04-23 2012-03-13 소시에다지 베네피시엔찌 이스라엘리따 브라질레이라 오스삐따우 알버트 아인슈타인 Method for isolating exosomes from biological solutions using iron oxide nanoparticles
WO2015048566A1 (en) * 2013-09-26 2015-04-02 The General Hospital Corporation Methods of isolating extracellular vesicles
WO2016033695A1 (en) * 2014-09-05 2016-03-10 Exerkine Corporation Exosome isolation
KR20160116802A (en) * 2015-03-31 2016-10-10 포항공과대학교 산학협력단 Isolation Method of Extracellular Vesicles by Aqueous Two-phase System

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAKAI ET AL.: "A novel affinity-based method for the isolation of highly purified extracellular vesicles", SCIENTIFIC REPORTS, vol. 6, 2016, pages 1 - 11, XP055566011 *
See also references of EP3660142A4 *

Also Published As

Publication number Publication date
WO2019022542A2 (en) 2019-01-31

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