WO2019004793A3 - Procédé de fabrication de peau artificielle, et peau artificielle - Google Patents

Procédé de fabrication de peau artificielle, et peau artificielle Download PDF

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Publication number
WO2019004793A3
WO2019004793A3 PCT/KR2018/007434 KR2018007434W WO2019004793A3 WO 2019004793 A3 WO2019004793 A3 WO 2019004793A3 KR 2018007434 W KR2018007434 W KR 2018007434W WO 2019004793 A3 WO2019004793 A3 WO 2019004793A3
Authority
WO
WIPO (PCT)
Prior art keywords
artificial skin
dermis
simulation layer
manufacturing
culturing
Prior art date
Application number
PCT/KR2018/007434
Other languages
English (en)
Korean (ko)
Other versions
WO2019004793A2 (fr
Inventor
길인섭
이성훈
배일홍
김형준
민대진
이태룡
Original Assignee
(주)아모레퍼시픽
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)아모레퍼시픽 filed Critical (주)아모레퍼시픽
Publication of WO2019004793A2 publication Critical patent/WO2019004793A2/fr
Publication of WO2019004793A3 publication Critical patent/WO2019004793A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de fabrication de peau artificielle, le procédé comprenant les étapes suivantes : mélange de fibroblastes et de collagène pour former une couche de simulation de derme; application de kératinocytes sur la couche de simulation de derme, suivie d'une mise en culture; et mise en culture de la couche de simulation de derme, à laquelle ont été appliqués des kératinocytes, dans un milieu de culture d'épiderme duquel un composant de pyruvate a été retiré.
PCT/KR2018/007434 2017-06-30 2018-06-29 Procédé de fabrication de peau artificielle, et peau artificielle WO2019004793A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0083517 2017-06-30
KR1020170083517A KR102213922B1 (ko) 2017-06-30 2017-06-30 인공피부 제조방법 및 인공피부

Publications (2)

Publication Number Publication Date
WO2019004793A2 WO2019004793A2 (fr) 2019-01-03
WO2019004793A3 true WO2019004793A3 (fr) 2019-03-28

Family

ID=64742569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/007434 WO2019004793A2 (fr) 2017-06-30 2018-06-29 Procédé de fabrication de peau artificielle, et peau artificielle

Country Status (2)

Country Link
KR (1) KR102213922B1 (fr)
WO (1) WO2019004793A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102452585B1 (ko) * 2019-06-28 2022-10-11 (주)아모레퍼시픽 인공피부 주름형성 장치, 이를 이용한 주름진 인공피부 제조방법, 상기 제조방법으로 제조된 주름진 인공피부 및 상기 주름진 인공피부를 이용한 피부 주름 개선 화합물 스크리닝 방법
KR102335878B1 (ko) * 2019-10-11 2021-12-06 (주)아모레퍼시픽 노화 인공피부, 이의 제조방법 및 이를 이용한 피부 항노화 물질 스크리닝 방법
KR102460180B1 (ko) * 2020-11-27 2022-10-27 숭실대학교산학협력단 연령별 구조 및 물성을 갖는 인공피부, 및 이의 제조방법
KR20230077302A (ko) 2021-11-25 2023-06-01 쓰리이솔루션 주식회사 기능성 혈관이 도입된 인공피부 및 그 제조방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6086789A (en) * 1996-03-18 2000-07-11 Case Western Reserve University Medical uses of pyruvates
US20010048917A1 (en) * 1998-03-09 2001-12-06 Warren Hoeffler Skin equivalent and methods of forming and using same
US20040052772A1 (en) * 1999-04-20 2004-03-18 Societe L'oreal S.A. Novel epidermis/dermis equivalents and aged skin equivalents shaped therefrom
KR20170055791A (ko) * 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100567655B1 (ko) * 2004-04-26 2006-04-04 사회복지법인 삼성생명공익재단 섬유아세포 배양용 배지, 상기 배지를 이용하여 진피대체물을 제조하는 방법 및 그에 의하여 제조되는 진피대체물, 상기 진피 대체물을 이용하여 피부 대체물을제조하는 방법 및 그에 의하여 제조되는 피부 대체물
KR100760989B1 (ko) * 2006-02-15 2007-10-04 주식회사 엠씨티티 생체적합성 스캐폴드에서 섬유아세포와 피부각질세포를공동 배양하는 방법
KR101874790B1 (ko) * 2011-11-23 2018-07-05 (주)아모레퍼시픽 멜라닌형성세포를 함유하는 인공피부 및 이의 제조방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6086789A (en) * 1996-03-18 2000-07-11 Case Western Reserve University Medical uses of pyruvates
US20010048917A1 (en) * 1998-03-09 2001-12-06 Warren Hoeffler Skin equivalent and methods of forming and using same
US20040052772A1 (en) * 1999-04-20 2004-03-18 Societe L'oreal S.A. Novel epidermis/dermis equivalents and aged skin equivalents shaped therefrom
KR20170055791A (ko) * 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WILEY , CHRISTOPHER D. ET AL.: "Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype", CELL METABOLISM, vol. 23, no. 2, 2016, pages 303 - 314, XP055586276 *

Also Published As

Publication number Publication date
KR102213922B1 (ko) 2021-02-08
WO2019004793A2 (fr) 2019-01-03
KR20190003061A (ko) 2019-01-09

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