WO2019003330A1 - 泳動媒体容器 - Google Patents
泳動媒体容器 Download PDFInfo
- Publication number
- WO2019003330A1 WO2019003330A1 PCT/JP2017/023686 JP2017023686W WO2019003330A1 WO 2019003330 A1 WO2019003330 A1 WO 2019003330A1 JP 2017023686 W JP2017023686 W JP 2017023686W WO 2019003330 A1 WO2019003330 A1 WO 2019003330A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- migration medium
- medium container
- seal
- syringe
- migration
- Prior art date
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
Definitions
- the present invention relates to an electrophoresis medium container suitable for use in an electrophoresis apparatus, for separating and analyzing nucleic acids, proteins and the like.
- a capillary electrophoresis apparatus in which a capillary is filled with a migration medium such as a polymer gel or a polymer solution has been widely used.
- Patent Document 1 describes that "the feeding of the migration medium is performed by pressing the seal part of the migration medium container from the outside".
- cost reduction is achieved by using a migration medium container as a resin molded product.
- the apparatus side is provided with a function of detecting the remaining amount of the migration medium container, and a function capable of removing the internal pressure in the migration medium container after liquid transfer. This makes it possible to manage the remaining amount in the migration medium container and to manage the amount of liquid transfer.
- an object of this invention is to provide the migration medium container which can stabilize a liquid feeding pressure, ensuring the sealability of a migration medium.
- the migration medium container of the present invention is a migration medium container provided with a syringe part which holds a migration medium, and a seal part which seals one end of the syringe part, and the seal part is A seal surface, a body portion, and a groove provided between the seal surface and the body portion, the seal surface being in contact with the inner wall of the syringe portion.
- the sealing surface comes in close contact with the inner wall of the container syringe unit, and the sealing performance can be secured.
- Device configuration outline diagram of the present invention Top view of the apparatus of the present invention AA cross section of the device Capillary array detail Migration medium container details Seal parts cross section Seal parts perspective view Sealed surface section enlarged view Sealed surface section enlarged view Seal surface cross section schematic drawing at the time of seal part insertion Cross section when connecting seal parts Perspective view of connectable seal parts Migration medium container installation detail Liquid transfer mechanism schematic Migration medium delivery operation details (initial state) Migration medium delivery operation details (plunger contact detection) Migration medium delivery operation detail drawing (phoresis medium injection) Pressure graph during polymer delivery Pressure graph during polymer delivery Cross-sectional view of a seal part provided with a seal surface at a plurality of places
- FIG. 1 is a schematic diagram of a capillary electrophoresis apparatus to which the present invention is applied.
- This apparatus can be roughly divided into two units, an auto sampler unit 16 at the lower part of the apparatus and an irradiation detection / constant temperature bath unit 17 at the upper part of the apparatus.
- the Y-axis drive 8 is mounted on the sampler base 7, the Z-axis drive 9 is mounted on the Y-axis drive 8, and the sample tray 11 is mounted on the Z-axis drive 9.
- the sample tray 11 can be driven to the Y axis and the Z axis.
- the user sets the migration medium container 2, the anode buffer container 3, the cathode buffer container 4, and the sample container 5 on the sample tray 11.
- the sample container 5 is set on the X-axis driver 10 mounted on the sample tray 11, and only the sample container 5 can be driven on the X-axis on the sample tray 11.
- the liquid feeding mechanism 6 is also mounted on the Z-axis driver 9. The liquid feeding mechanism 6 is disposed below the migration medium container 2.
- the irradiation detection / constant temperature bath unit 17 includes a constant temperature bath unit 12 and a constant temperature bath door 14 so that the inside can be maintained at a constant temperature.
- An irradiation detection unit 15 is mounted behind the thermostatic bath unit 12 so that detection during electrophoresis can be performed.
- the capillary array 1 is set in the constant temperature bath unit 12, and electrophoresis is performed in the constant temperature bath unit 12 while keeping the capillary array 1 at a constant temperature, and detection is performed by the irradiation detection unit 15.
- the thermostatic bath unit 12 is also mounted with an electrode 13 for dropping to GND when a high voltage is applied for electrophoresis.
- the capillary array 1 is fixed to the thermostatic bath unit 12.
- the electrophoresis medium container 2, the anode buffer container 3, the cathode buffer container 4, and the sample container 5 can be driven to the YZ axis by the auto sampler unit 16, and only the sample container 5 is further driven to the X axis. I can do it.
- the electrophoresis medium container 2, the anode side buffer solution container 3, the cathode side buffer solution container 4 and the sample container 5 can be automatically connected to an arbitrary position by the movement of the autosampler unit 16 to the fixed capillary array 1 .
- FIG. 2 shows a top view of the capillary electrophoresis apparatus.
- the anode-side buffer container 3 set on the sample tray 11 includes an anode-side washing tank 21, an anode-side electrophoresis buffer tank 22, and a sample introduction buffer tank 23.
- the cathode side buffer solution container 4 includes a waste liquid tank 24, a cathode side cleaning tank 25, and a cathode side electrophoresis buffer tank 26.
- the migration medium container 2, the anode buffer container 3, the cathode buffer container 4, and the sample container 5 are disposed in the positional relationship as illustrated.
- the positional relationship between the anode side and the cathode side at the time of connection with the capillary array 1 is “migration medium container 2-waste liquid tank 24”, “anode side cleaning tank 21-cathode side cleaning tank 25”, “anode side electricity
- FIG. 3 shows a cross-sectional view taken along the line AA in FIG.
- the migration medium container 2 is inserted and set in the guide 31 embedded in the sample tray 11. Further, the liquid transfer mechanism 6 is disposed such that the plunger 32 provided in the liquid transfer mechanism 6 is located below the migration medium container 2.
- the right side of the capillary array 1 in FIG. 3 is the cathode side, and the left side is the anode side.
- the autosampler unit 16 is moved to the position of “Anode-Side Electrophoresis Buffer Tank 22-Cathode-Side Electrophoresis Buffer Tank 26”, and a high voltage is applied to the cathode-side capillary array 1. Electrophoresis is performed by flowing the electrode 13 to GND through the anode buffer container 3.
- FIG. 4 shows a detailed view of the capillary array 1.
- the capillary array 1 includes a capillary 41 which is a glass tube having an inner diameter of about ⁇ 50 ⁇ m, and a detection unit 42 is attached to the capillary 41. The detection unit 42 detects this detection unit 42.
- a load header 46 and an SUS pipe 47 are attached.
- the material of the load header 46 is, for example, PBT resin, which is a resin having a high insulation property and a high comparison tracking index.
- PBT resin is a resin having a high insulation property and a high comparison tracking index.
- the capillaries 41 are respectively passed through the SUS pipe 47 and fixed. On the anode side, a plurality of capillaries 41 are put together by a capillary head 43.
- the capillary head 43 has a capillary head tip 45 which is needle-shaped at an acute angle, and a capillary head boss 44 whose outer diameter is larger than that of the capillary head tip 45.
- the material of the capillary head 43 is preferably a PEEK resin or the like which is hard to chip and is rigid and which is a resin having high stability to chemicals and analysis.
- the detection unit 42 performs positioning with high accuracy so that it can be detected by the irradiation detection unit.
- the load header 46 is fixed so as to be conductive with the point to which the high voltage is applied.
- the capillary head 43 is firmly fixed so that the tip 45 of the capillary head is directed downward and can bear the load.
- the positional relationship between the cathode side and the anode side at the time of fixation is such that the plurality of capillaries 41 do not overlap when set in the apparatus.
- FIG. 5 shows a structural view of the migration medium container 2.
- the migration medium container 2 has a syringe unit 51.
- the seal component 52 is inserted from the top (the upper side in the drawing) of the syringe unit 51 so as to be pushed in, and is moved to the bottom of the syringe unit 51.
- a rubber plug 53 is placed on the top of the syringe unit 51, and the cap 54 is turned to seal. Further, a film 55 is attached and sealed on the cap 54.
- the inside of the migration medium container 2 is filled with the migration medium 56, but the air 57 entering at this time is made to be accumulated in the upper part of the syringe unit 51.
- the material of the migration medium container 2 is preferably a COP resin or the like which is a resin capable of being thin-walled and having little influence on the material properties due to temperature change.
- FIG. 6A shows a structural view of the seal component 52 inserted into the syringe unit 51.
- the seal component 52 slides in the syringe unit 51 when the migration medium 56 is fed.
- the shape of the sealing part 52 is a cylindrical shape provided with a bottom portion 59 as shown in FIG. 6B, and the cross section is U-shaped inward of the migration medium container 2. Since the bottom portion 59 is provided, it has a role of sealing the migration medium 56 inside the migration medium container 2.
- the outer diameter of the seal surface 58 of the seal component 52 is slightly larger than the inner diameter of the syringe portion 51. Therefore, when inserted, the seal surface 58 is crushed and comes in close contact with the inner wall of the syringe unit 51.
- the seal component 52 has a U-shaped cross section, and the seal surface 58 is provided at the top, so that when the internal pressure of the migration medium container 2 is increased, the seal surface 58 extends outward and the syringe portion 51 is Seal.
- the sealing surface 58 may be flat or curved.
- the body portion 61 has a diameter smaller than the outer diameter of the sealing surface 58, and has an outer diameter not in contact with the inner wall of the syringe portion 51 even when it expands outward when the internal pressure of the migration medium container 2 rises. Thereby, only the seal surface 58 contacts the inner wall of the syringe unit 51.
- the portion in contact with the inner wall of the syringe portion 51 is limited to the sealing surface 58, and the change in the contact area between the inner wall of the syringe portion 51 and the seal component 52 is reduced to change the sliding resistance during liquid transfer. It can be suppressed and the pressure in the liquid delivery can be stabilized.
- a groove 60 is provided between the sealing surface 58 and the body portion 61.
- the seal surface 58 is easily spread to the outside when the internal pressure of the migration medium container 2 rises, so the seal surface 58 and the inner wall of the syringe portion 51 can be more closely attached, and the sealability is improved.
- the groove 60 may be provided inside the seal component 58 as long as the thickness of the seal component 52 is smaller than that of the seal surface 58 and the body portion 61.
- the measurement result of liquid feeding pressure is shown in FIG. 15 (a) and FIG.15 (b).
- 15A shows the case where the body portion 61 is in contact with the inner wall of the syringe portion 51
- FIG. 15B shows the case where the body portion 61 is not in contact with the inner wall of the syringe portion 51.
- the seal surface 58 is provided on the top of the seal component 52, as shown in FIG. 16, the body seal surface 62 may be provided at a plurality of locations with respect to the seal component 52. This makes it possible to further prevent the migration medium 56 from leaking.
- the material of the seal part 52 has a track record of being used for sealing the fluid of the sliding part, etc., and the expansion coefficient is larger than that of the material of the syringe part 51, and it is easy to spread outside when the internal pressure of the migration medium container 2 increases. Molecular PE resin or the like is desirable, but a rubber material may be used.
- FIG. 10 shows a detailed view of the installation of the migration medium container 2.
- the film 55 attached to the cap 54 is peeled off. Thereafter, it is set on the guide 31 embedded in the sample tray 11 and fixed from above so as not to be lifted.
- the guide 31 has high rigidity, and plays a role of suppressing deformation of the migration medium container 2 until it contacts the guide 31.
- the gap between the outer diameter of the syringe unit 51 and the inner diameter of the guide 31 is made as small as possible. The smaller the gap, the better.
- the gap between the outer diameter of the syringe portion 51 of the resin molded product and the inner diameter of the guide 31 which is a machined product is such that there is no processing excess.
- FIG. 11 shows a schematic view of the liquid feeding mechanism 6.
- the stepping motor 102 rotates according to the input pulse number, rotates the drive screw 91, and moves the nut 93 in a straight line.
- the driving method of the stepping motor 102 is, for example, 1-2 phase excitation.
- the nut 93 is coupled to the slider 92, and the slider 92 is coupled to the plunger 32.
- Position control of the plunger 32 is performed by a rotary encoder 103 integrated with the stepping motor 102.
- the slider 92 is connected to the linear guide 98 and is movable in the drive screw 91 axial direction.
- a detection plate 99 is coupled to the slider 92, and is detected by the origin sensor 100 fixed to the liquid feeding mechanism base 101.
- the detection position of the origin sensor 100 is the origin position of the plunger 32.
- a torque limiter 104 is attached to the drive screw 91 as an external load that imparts rotational resistance.
- the torque limiter 104 has a structure in which the inner ring 96 and the outer ring 95 are coaxially fitted.
- the inner ring 96 of the torque limiter 104 is hollow. Further, the inner ring 96 and the outer ring 95 can rotate independently. There is a constant rotational resistance between the inner ring 96 and the outer ring 95.
- a magnet type that is less susceptible to wear is desirable.
- the drive screw 91 and the inner ring 96 rotate in synchronization with each other by inserting the drive screw 91 through the hollow portion of the inner ring 96 and engaging the inner ring 96 with the parallel pin 97 vertically penetrating the drive screw 91.
- the outer ring 95 is fixed so as not to rotate by the torque limiter outer ring restraining 94 fixed to the liquid feeding mechanism base 101.
- This structure makes it possible to provide the drive screw 91 with resistance.
- the outer ring 95 is fixed, and a torque limiter 104 that requires 45 mN ⁇ m of torque when the inner ring 96 is rotated is used.
- FIGS. 1 The positional relationship between the plunger 32 and the migration medium container 2 and the capillary head 43 at each point is shown in FIGS.
- the migration medium container 2 is set on the guide 31.
- the plunger 32 of the liquid feeding mechanism 6 is disposed immediately below the migration medium container 2.
- FIG. 13 shows a diagram of the initial state of a series of movements of the migration medium 56 injection operation.
- the seal component 52 inserted in the migration medium container 2 can be slid by the movement of the plunger 32.
- the liquid feeding mechanism 6 drives the plunger 32 to bring the plunger 32 into contact with the bottom 59 of the seal component 52.
- FIG. 14 shows a state in which the electrophoresis medium 56 is being filled in the capillary 41.
- the seal part 52 is further pushed up to feed the migration medium 56 to the capillary 41.
- the inside of the migration medium container 2 becomes high pressure, and the respective parts of the migration medium container 2 expand outward.
- the deformation of the syringe portion 51 of the migration medium container 2 is suppressed by the guide 31, and the deformation of the rubber plug 53 is suppressed by the capillary head 43.
- the sealing part 52 is deformed by the internal pressure, it is deformed so as to spread outward and is more sealed.
- the risk of the leakage of the migration medium 56 due to the deformation of the syringe unit 51 can be reduced by making the seal component 52 a shape and strength in which the expansion coefficient is more easily deformed than the syringe unit 51 of the migration medium container 2. It can.
- the sealing surface 58 may be provided with a multistage structure.
- FIG. 7A shows a diagram of a multistage structure.
- the sealing surface 58 has a multistage structure of a recess 71 and a protrusion 72 along the vertical direction, and when the seal component 52 is inserted into the inside of the syringe portion 51, the protrusion 72 is crushed and closely attached as shown in FIG. Even if the migration medium 56 gets into the space between it and the inner wall of the syringe unit 51, its leakage pressure can be gradually lowered, so that it is possible to prevent downward leakage. Furthermore, by narrowing the tip of the protrusion 72, the protrusion 72 is easily crushed by the internal pressure, and the protrusion 72 and the syringe can be more closely attached.
- the seal surface on which the multistage structure is provided may be a flat surface as shown in FIG. 7 (a) or a curved surface as shown in FIG. 7 (b).
- one seal component 52 is used, but a plurality of seal components 52 may be connected.
- FIG. 9 (a) is a cross-sectional view of a state in which two seal components 52 (consisting of an upper seal 81 and a lower seal 82) are longitudinally connected
- FIG. 9 (b) is an upper seal 81 before connection. It is a perspective view which shows the lower side seal
- FIG. The bottom of the upper seal 81 is provided with a rod-like insertion portion 85, and the insertion portion 85 of the upper seal 81 is connected to be inserted into the lower seal 82.
- the sealing surfaces become two surfaces 83 and 84, and the sealing property of the migration medium 56 can be improved.
- the seal surface of the upper seal 81 and the lower seal 82 is assumed to be a multistage structure of the concave portion 71 and the convex portion 72 as shown in FIG. 7A and FIG. Even if the seal surface 83 has a flaw or chip and the migration medium 56 leaks downward, the lower seal 82 can prevent the migration medium 56 from leaking to the outside of the migration medium container 2. Further, by providing a distance between the seal surface 83 of the upper seal 81 and the seal surface 84 of the lower seal 82 by the insertion portion 85, more migration medium is confined when the migration medium 56 leaks. It is possible to prevent leakage further.
- Example 1 although two seal parts were connected, three or more of this connection may be connected. In this case, for example, a plurality of upper seals 81 are provided, and a lower seal 82 is provided at the lowermost portion.
- the sealing surface comes in close contact with the inner wall of the container syringe unit, and the sealing performance can be ensured.
- the sealing performance can be ensured.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Electrochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
2 泳動媒体容器
3 陽極側緩衝液容器
4 陰極側緩衝液容器
5 サンプル容器
6 送液機構
7 サンプラーベース
8 Y駆動体
9 Z駆動体
10 X駆動体
11 サンプルトレイ
12 恒温槽ユニット
13 電極
14 恒温槽ドア
15 照射検出ユニット
16 オートサンプラーユニット
17 照射検出/恒温槽ユニット
21 陽極側洗浄槽
22 陽極側電気泳動用緩衝液槽
23 陽極側サンプル導入用緩衝液槽
24 廃液槽
25 陰極側洗浄槽
26 陰極側電気泳動用緩衝液槽
31 ガイド
32 プランジャ
41 キャピラリ
42 検出部
43 キャピラリヘッド
44 キャピラリヘッドボス
45 キャピラリヘッド先端
46 ロードヘッタ
47 SUSパイプ
51 シリンジ部
52 シール部品
53 ゴム栓
54 キャップ
55 フィルム
56 泳動媒体
57 空気
58 シール面
59 シール底部
60 溝
61 胴体部
62 胴体部シール面
71 凹部
72 凸部
81 上側シール
82 下側シール
83 上側シール面
84 下側シール面
85 挿し込み部分
91 駆動ねじ
92 スライダ
93 ナット
94 トルクリミッタ外輪抑え
95 トルクリミッタ外輪
96 トルクリミッタ内輪
97 平行ピン
98 リニアガイド
99 検知板
100 原点センサ
101 送液機構ベース
102 ステッピングモータ
103 ロータリーエンコーダ
104 トルクリミッタ
Claims (10)
- 泳動媒体を保持するシリンジ部と、
前記シリンジ部の一端を封止するシール部品と、
を備える泳動媒体容器であって、
前記シール部品は、
シール面と、
胴体部と、
前記シール面と前記胴体部との間に設けられた溝とを有し、
前記シール面が前記シリンジ部内壁と接触する、
泳動媒体容器。 - 請求項1に記載の泳動媒体容器であって、
前記シール面の外径が、シール部品の他部分の外径より大きく、シリンジ部の内径より大きい、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール面が、鉛直方向に沿って凹凸の多段構造である、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール面の少なくとも一つが、シール部品の最上部に設けられている、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール面が、内圧により外側に広がる、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール面が、平面もしくは曲面を有している、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール部品の方が、前記シリンジ部と比較して膨張係数が大きい、
泳動媒体容器。 - 請求項2に記載の泳動媒体容器であって、
前記シール部品が複数個、鉛直方向に連結している、
泳動媒体容器。 - 請求項3に記載の泳動媒体容器であって、
前記シール面の多段構造の凸部が先端に向かって狭まっている、
泳動媒体容器。 - 請求項8に記載の泳動媒体容器であって、
前記シール部品が複数個、棒状の挿し込み部分によって連結される、
泳動媒体容器。
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2017/023686 WO2019003330A1 (ja) | 2017-06-28 | 2017-06-28 | 泳動媒体容器 |
CN201780092540.9A CN110799836B (zh) | 2017-06-28 | 2017-06-28 | 泳动介质容器 |
US16/620,961 US11243187B2 (en) | 2017-06-28 | 2017-06-28 | Electrophoretic medium container |
GB1917981.1A GB2577222B (en) | 2017-06-28 | 2017-06-28 | Electrophoretic medium container |
DE112017007534.3T DE112017007534T5 (de) | 2017-06-28 | 2017-06-28 | Elektrophoresemediumbehälter |
JP2019526452A JP6754497B2 (ja) | 2017-06-28 | 2017-06-28 | 泳動媒体容器 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2017/023686 WO2019003330A1 (ja) | 2017-06-28 | 2017-06-28 | 泳動媒体容器 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2019003330A1 true WO2019003330A1 (ja) | 2019-01-03 |
Family
ID=64741982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2017/023686 WO2019003330A1 (ja) | 2017-06-28 | 2017-06-28 | 泳動媒体容器 |
Country Status (6)
Country | Link |
---|---|
US (1) | US11243187B2 (ja) |
JP (1) | JP6754497B2 (ja) |
CN (1) | CN110799836B (ja) |
DE (1) | DE112017007534T5 (ja) |
GB (1) | GB2577222B (ja) |
WO (1) | WO2019003330A1 (ja) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012120585A (ja) * | 2010-12-06 | 2012-06-28 | Fine Rubber Kenkyusho:Kk | シリンジ用部材、該シリンジ用部材を用いた医療用シリンジ、及び該シリンジ用部材の製造方法 |
WO2012118194A1 (ja) * | 2011-03-02 | 2012-09-07 | 電気化学工業株式会社 | 樹脂製バレルを有するシリンジ内にヒアルロン酸又はその塩を含む水溶液を充填してなるプレフィルドシリンジ |
JP2014047828A (ja) * | 2012-08-30 | 2014-03-17 | Sumitomo Rubber Ind Ltd | 積層ガスケット |
WO2016157272A1 (ja) * | 2015-03-27 | 2016-10-06 | 株式会社 日立ハイテクノロジーズ | 電気泳動装置および電気泳動方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1273192B (it) * | 1994-05-10 | 1997-07-07 | Musetta Angela | Siringa monouso di sicurezza |
JP2000009690A (ja) * | 1998-06-24 | 2000-01-14 | Hitachi Ltd | キャピラリ電気泳動用溶液充填装置 |
WO2009158613A1 (en) | 2008-06-26 | 2009-12-30 | West Pharmaceutical Services, Inc. | Method of coating polyxylylene onto flouropolymer surfaces and devices coated thereby |
KR101924310B1 (ko) * | 2011-12-15 | 2018-11-30 | 에스에이치엘 그룹 에이비 | 자동 주입 장치 |
JP5391350B2 (ja) * | 2013-04-15 | 2014-01-15 | 株式会社日立ハイテクノロジーズ | 電気泳動媒体用容器及び電気泳動装置の駆動方法 |
-
2017
- 2017-06-28 WO PCT/JP2017/023686 patent/WO2019003330A1/ja active Application Filing
- 2017-06-28 DE DE112017007534.3T patent/DE112017007534T5/de active Pending
- 2017-06-28 JP JP2019526452A patent/JP6754497B2/ja active Active
- 2017-06-28 US US16/620,961 patent/US11243187B2/en active Active
- 2017-06-28 CN CN201780092540.9A patent/CN110799836B/zh active Active
- 2017-06-28 GB GB1917981.1A patent/GB2577222B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012120585A (ja) * | 2010-12-06 | 2012-06-28 | Fine Rubber Kenkyusho:Kk | シリンジ用部材、該シリンジ用部材を用いた医療用シリンジ、及び該シリンジ用部材の製造方法 |
WO2012118194A1 (ja) * | 2011-03-02 | 2012-09-07 | 電気化学工業株式会社 | 樹脂製バレルを有するシリンジ内にヒアルロン酸又はその塩を含む水溶液を充填してなるプレフィルドシリンジ |
JP2014047828A (ja) * | 2012-08-30 | 2014-03-17 | Sumitomo Rubber Ind Ltd | 積層ガスケット |
WO2016157272A1 (ja) * | 2015-03-27 | 2016-10-06 | 株式会社 日立ハイテクノロジーズ | 電気泳動装置および電気泳動方法 |
Also Published As
Publication number | Publication date |
---|---|
JPWO2019003330A1 (ja) | 2020-04-16 |
JP6754497B2 (ja) | 2020-09-09 |
GB2577222B (en) | 2022-05-25 |
US20200200705A1 (en) | 2020-06-25 |
US11243187B2 (en) | 2022-02-08 |
GB2577222A (en) | 2020-03-18 |
CN110799836A (zh) | 2020-02-14 |
CN110799836B (zh) | 2022-11-22 |
GB201917981D0 (en) | 2020-01-22 |
DE112017007534T5 (de) | 2020-01-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111579623B (zh) | 电泳装置及电泳方法 | |
US20160216235A1 (en) | Electrophoresis Medium Receptacle and Electrophoresis Apparatus | |
WO2019003330A1 (ja) | 泳動媒体容器 | |
US8177951B2 (en) | Electrophoresis apparatus and pump mechanism used in the same | |
CN109791123B (zh) | 驱动螺杆装置、送液机构以及送液方法 | |
US11592352B2 (en) | System and method for high voltage leak detection | |
JP6770650B2 (ja) | キャピラリ電気泳動装置 | |
JP2014163714A (ja) | 蒸発防止膜 | |
GB2584263A (en) | Electrophoresis device and electrophoresis method | |
JP2009180569A (ja) | キャピラリ電気泳動装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 17915646 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2019526452 Country of ref document: JP Kind code of ref document: A Ref document number: 201917981 Country of ref document: GB Kind code of ref document: A Free format text: PCT FILING DATE = 20170628 |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 17915646 Country of ref document: EP Kind code of ref document: A1 |