Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although exemplary methods or materials are listed herein, other similar or equivalent ones are also within the scope of the present invention. All numeral values referred to herein in connection with embodiments of the present disclosure should be understood as approximate values, though not explicitly specified with the term such as "about". All publications disclosed as references herein are incorporated in their entirety by reference.
According to an aspect of the present disclosure, an oral composite tablet includes: an ezetimibe wet granule portion including ezetimibe; and a rosuvastatin mixture portion including rosuvastatin or a pharmaceutically acceptable salt thereof, wherein the ezetimibe wet granule portion includes, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000, and the rosuvastatin mixture portion includes a diluent, wherein the diluent comprises a water-soluble diluent and a water-insoluble diluent, the water-insoluble diluent having a particle size (d90) of about 98 μm to about 229 μm corresponding to 90% of total particles in a cumulative particle size distribution.
As used herein, the term "d90" refers to an average particle size corresponding to 90% of total particles in a cumulative size distribution, as generally understood in the art. For example, when "d90 is 100 μm," this may indicate that 90% or more of active ingredient particles have an equivalent spherical volume diameter of about 100 μm or less, as measured using, for example, a Mastersizer (available from Malvern Instruments) or a HELOS particle size analyzer (available from Sympatec). However, embodiments are not limited thereto, and other particle size analyzers may also be used.
In an aspect of pharmacokinetics, since Tmax of ezetimibe is as short as less than 1 hour, after administration in the form of a tablet, rapid disintegration and dissolution of the tablet are required for rapid absorption to attain a desired therapeutic effect. To obtain a rapid initial dissolution, granules containing the active ingredient may be prepared as bulky granules having a small density. However, such bulky granules may cause a problem such as capping during tableting or a large mass deviation in the prepared tablets. On the other hand, when active ingredient-containing granules are prepared without the occurrence of problems during tableting, it may take a long time to disintegrate such granules, and a rapid initial dissolution rate cannot be expected. Thus, it may not be easy to prepare a tablet of ezetimibe having a high initial dissolution rate with improved productivity.
In the oral composite tablet according to one or more embodiments, the ezetimibe wet granule portion may include, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000, thereby ensuring an improved initial dissolution rate of the ezetimibe of the composite tablet while also securing an angle of repose of granules of about 35° to 40° so as to attain appropriate flowability of ezetimibe wet granules. In the oral composite tablet according to one or more embodiments, when the ezetimibe wet granule portion includes, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000, as a result of a dissolution test performed according to Dissolution method II in the Korean Pharmacopoeia at a paddle rotation speed of about 75 rpm, the ezetimibe in the oral composite tablet was found to have a dissolution rate of about 90% or greater in about 30 minutes at a temperature of about 37.5° in a sodium acetate buffer solution of a pH of about 4.5 containing about 0.45% sodium lauryl sulfate (see Test Example 2 and Table 7). As a result of evaluating physical properties of the ezetimibe wet granules, the ezetimibe wet granules were found to have an angle of repose in a range of about 35° to about 40° at which productivity is secured during tableting (see Test Example 1 and Table 5). Therefore, the oral composite tablet according to one or more embodiments may have improved pharmaceutical advantages, including an improved high initial dissolution rate and improved productivity.
In some embodiments, the ezetimibe wet granule portion may include, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000 in an amount of about 0.2 parts to about 0.6 parts by weight with respect to 1 part by weight of the ezetimibe.
In the oral composite tablet according to one or more embodiments, the rosuvastatin mixture portion may include a diluent including a water-soluble diluent and a water-insoluble diluent, the water-insoluble diluent having a d90 of about 98 μm to about 229 μm, thereby ensuring that the ezetimibe of the oral composite tablet has an improved initial dissolution rate while also securing an angle of repose in a range of about 35° to about 40° with respect to a final mixture of the ezetimibe wet granule portion and the rosuvastatin mixture portion such that the final mixture may have appropriate flowability during tableting. In the oral composite tablet according to one or more embodiments, when the water-insoluble diluent of the rosuvastatin mixture portion has a d90 of about 98 μm to about 229 μm, as a result of a dissolution test performed according to Dissolution method II in the Korean Pharmacopoeia at a paddle rotation speed of about 75 rpm, the ezetimibe in the oral composite tablet was found to have a dissolution rate of about 90% or greater in about 30 minutes at a temperature of about 37.5° in a sodium acetate buffer solution of a pH of about 4.5 containing about 0.45% sodium lauryl sulfate (see Test Example 2 and Table 7), and the final mixture of the ezetimibe wet granule portion and the rosuvastatin mixture portion had an angle of repose in a range of about 35° to about 40° (see Test Example 1 and Table 4). Accordingly, the oral composite tablet according to one or more embodiments may have an improved initial resolution rate of ezetimibe and improved productivity during tableting when the composite tablet is prepared by incorporating a water-insoluble diluent having a certain particle size range into the rosuvastatin mixture portion.
The oral composite tablet according to one or more embodiments may have an effect of reducing deposition of the water-insoluble diluent in a dissolution vessel of a dissolution tester, thus remarkably reducing a deviation in dissolution rate of the ezetimibe, and accordingly ensuring a suitable quality evaluation result for the oral composite tablet. The oral composite tablet according to one or more embodiments may include both a water-soluble diluent and a water-insoluble diluent. In general, during a dissolution test of a composite tablet, a water-insoluble diluent tends to be deposited in a dissolution vessel and not dissolved. The deposition of the water-insoluble diluent in the dissolution vessel may delay dissolution of the ezetimibe, the ezetimibe being nearly insoluble in water, in a dissolution medium during a dissolution test for quality evaluation of the composite tablet, consequently leading to an increased deviation in dissolution rate. In some embodiments, the rosuvastatin mixture portion of the oral composite tablet may include a water-soluble diluent and a water-insoluble diluent, the water-insoluble diluent having a d90 of about 98 μm to about 229 μm, so that deposition of the water-insoluble diluent in a dissolution vessel may be remarkably reduced. Consequently, the dissolution rate of the ezetimibe may be increased, with a reduced deviation in the dissolution rate (see Test Example 2, Table 6, Test Example 3, and Table 8). As described above, by including the water-insoluble diluent having a certain particle size range in the rosuvastatin mixture portion, the oral composite tablet may have an increased initial dissolution rate of the ezetimibe and a remarkably reduced deviation in dissolution of the ezetimibe in a dissolution test, and thus may be evaluated as having suitable quality in terms of a dissolution rate of ezetimibe in the composite tablet.
The water-insoluble diluent in the rosuvastatin mixture portion according to one or more embodiments, having a d90 of about 98 to about 229 μm, may be any diluent known in the art. For example, the water-insoluble diluent may include microcrystalline cellulose, starch, pregelatinized starch, dicalcium phosphate (DCP), low-substituted hydroxypropyl cellulose, or any combination thereof. However, embodiments are not limited thereto. In some embodiments, the water-insoluble diluents may be microcrystalline cellulose.
In some embodiments, the water-insoluble diluent may be a water-insoluble diluent having a d90 of about 98 μm to about 229 μm and a d50 of about 44 μm to about 108 μm, wherein the d50 refers to a particle size corresponding to 50% in a cumulative particle size distribution. In some embodiments, the water-insoluble diluent having a d90 of about 98 μm to about 229 μm may be microcrystalline cellulose having a d90 of about 98 μm to about 229 μm, and a d50 of about 44 μm to about 108 μm.
The water-insoluble diluent may be in an amount of about 2 parts to about 10 parts by weight with respect to 1 part by weight of the rosuvastatin.
The water-soluble diluent may be any diluent known in the art. For example, the water-soluble diluent may be selected from the group consisting of lactose, mannitol, carboxymethyl cellulose, and any combination thereof. However, embodiments are not limited thereto.
The water-soluble diluent may be in an amount of about 2 parts to about 10 parts by weight with respect to 1 part by weight of the rosuvastatin.
As used herein, the term "wet granule portion" may refer to a mixture of granules prepared in a wet condition, and the term "mixture portion" may refer to a simple mixture in a non-granular form or a mixture of granules prepared using any method excluding a wet granulation.
In some embodiments, the rosuvastatin mixture portion may be in the form of a simple mixture or a dry granular form. According to test results, when a rosuvastatin mixture portion was prepared as a simple mixture or in a dry granular form without using water as in one or more embodiments and then used to prepare a composite tablet, the composite tablet was found to include a remarkably reduced amount, for example, about less than 1.5%, of rosuvastatin lactone-related compounds after storage under a stressed condition, compared with a composite tablet obtained using a rosuvastatin mixture portion prepared in a wet granular form using water (see Test Example 4 and Table 9). Thus, the oral composite tablet according to one or more embodiments may have remarkably improved stability of the rosuvastatin due to being produced through a process in which exposure of the rosuvastatin to water is minimized.
In the oral composite tablet according to one or more embodiments, the ezetimibe wet granule portion and the rosuvastatin mixture portion may each further include a pharmaceutically acceptable additive selected from a binder, a disintegrant, a glidant, and any combination thereof.
The binder, the disintegrant, and the glidant may be selected from any known pharmaceutically acceptable additives commonly used in the art. For example, the binder may be selected from the group consisting of povidone, hypromellose, hydroxypropyl cellulose, copovidone, and any combination thereof. However, embodiments are not limited thereto. The disintegrant may be selected from the group consisting of crospovidone, croscarmellose sodium, sodium starch glycolate, low-substituted hydroxypropyl cellulose, and any combination thereof. However, embodiments are not limited thereto. The glidant may be selected from the group consisting of magnesium stearate, talc, light anhydrous silicic acid, sodium stearyl fumarate, and any combination thereof. However, embodiments are not limited thereto.
In the oral composite tablet according to one or more embodiments, an amount of the ezetimibe as a first active ingredient in the oral composite tablet may be from about 5 mg to about 20 mg, and in some other embodiments, about 5 mg to about 10 mg, in a unit dosage form of the composite tablet.
The oral composite tablet according to one or more embodiments may include rosuvastatin or a pharmaceutically acceptable salt thereof as a second active ingredient. The rosuvastatin may be in the form of a free base or a pharmaceutically acceptable salt thereof. For example, the pharmaceutically acceptable salt may be a calcium salt, a magnesium salt, a strontium salt, or the like. For example, the rosuvastatin may be a rosuvastatin calcium salt. However, embodiments are not limited thereto.
An amount of the rosuvastatin or a pharmaceutically acceptable salt thereof may be about 2.5 mg to about 40 mg, and in some embodiments, about 5 mg to about 20 mg, as a free base in a unit dosage form of the oral composite tablet according to one or more embodiments.
The oral composite tablet may be any tablet form including the ezetimibe wet granule portion and the rosuvastatin mixture portion, for example, in the form of a single-layered tablet, a double-layered tablet, or a press-coated tablet. However, embodiments are not limited thereto.
In the oral composite tablet according to one or more embodiments, a dissolution rate of the ezetimibe may be about 90% or greater in about 30 minutes at a temperature of about 37.5℃ in a sodium acetate buffer solution having a pH of about 4.5 containing about 0.45% sodium lauryl sulfate, as measured at a paddle rotation speed of about 75 rpm in a dissolution test of the oral composite tablet performed according to Dissolution method II in the Korean Pharmacopoeia.
In the oral composite tablet according to one or more embodiments, a dissolution rate of the ezetimibe may be about 20% or greater in about 30 minutes at a temperature of about 37.5℃ in simulated gastric fluid (SGF) of a pH of about 1.2, as measured at a paddle rotation speed of about 50 rpm in a dissolution test of the oral composite tablet performed according to Dissolution method II in the Korean Pharmacopoeia.
In the oral composite tablet according to one or more embodiments, a total amount of rosuvastatin lactone-related compounds may be less than about 1.5%, as measured in a stress testing of the oral composite tablet under stressed conditions at a temperature of about 60℃ for about 2 weeks.
According to another aspect of the present disclosure, a method of preparing the oral composite tablet according to any of the embodiments includes: preparing the ezetimibe wet granule portion by wet-granulating the ezetimibe together with a pharmaceutically acceptable additive including, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000; and tableting the ezetimibe wet granule portion together with the rosuvastatin mixture portion including the rosuvastatin or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable additive, wherein the pharmaceutically acceptable additive as a diluent includes a water-soluble diluent and a water-insoluble diluent, the water-insoluble diluent having a particle size (d90) of about 98 μm to about 229 μm corresponding to 90% in a cumulative particle size distribution of total particles.
The above descriptions of the oral composite tablet according to the one or more embodiments may apply to embodiments of the method of preparing the oral composite tablet.
In some embodiments of the method, the wet-granulating to prepare the ezetimibe wet granule portion may be performed using fluid-bed granulation, spray-drying, or a high-speed mixer.
In some embodiments of the method, the rosuvastatin mixture portion including the rosuvastatin or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable additive may be a simple mixture formed using simple mixing or a dry granule form prepared using dry granulation.
In some embodiments of the method, the ezetimibe wet granule portion may be prepared so as to include, as a binder, the povidone having a weight average molecular weight of about 30,000 to about 50,000 in an amount of about 0.2 parts to about 0.6 parts by weight with respect to 1 part by weight of the ezetimibe.
In some embodiments of the method, the ezetimibe wet granule portion and the rosuvastatin mixture portion may each further include about 0.5 parts to about 50 parts by weight of a diluent, about 0.1 parts to about 20 parts by weight of a binder, about 0.1 parts to about 40 parts by weight of a disintegrant, about 0.1 parts to about 3 parts by weight of a glidant, each with respect to 1 part by weight of the ezetimibe, or any combination thereof.
In some embodiments, the method of preparing the oral composite tablet according to any of the embodiments may include: (i) preparing the ezetimibe wet granule portion by wet-granulating the ezetimibe together with a pharmaceutically acceptable additive including, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000; (ii) preparing the rosuvastatin mixture portion in the form of a simple mixture or dry granules, the rosuvastatin mixture portion including the rosuvastatin or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable additive; and (iii) preparing single-layered tablets using a general tablet preparation method from a final mixture including the ezetimibe wet granule portion prepared in step (i) and the rosuvastatin mixture portion prepared in step (ii).
In some other embodiments, the method of preparing the oral composite tablet according to any of the embodiments may include: (i) preparing the ezetimibe wet granule portion by wet-granulating the ezetimibe together with a pharmaceutically acceptable additive including, as a binder, povidone having a weight average molecular weight of about 30,000 to about 50,000, and tableting the ezetimibe wet granule portion into a tablet; (ii) preparing the rosuvastatin mixture portion in the form of a simple mixture or dry granules, the rosuvastatin mixture portion including the rosuvastatin or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable additive; and (iii) preparing a double-layered tablet using a general tableting method with a double-layer tableting machine, the double-layered tablet including the tablet prepared in step (i) as a first layer and the rosuvastatin mixture portion prepared in step (ii) as a second layer.
In some embodiments, the preparing of the rosuvastatin mixture portion as dry granules may be performed using a common method known in the art. In some embodiments, the preparing of the rosuvastatin mixture portion as dry granules may include dry-granulating a simple mixture of the rosuvastatin or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable additive using a roller compactor, and then sieving the resulting dry granules using an oscillator.
MODE OF THE INVENTION
One or more embodiments of the present disclosure will now be described in detail with reference to the following examples. However, these examples are only for illustrative purposes and are not intended to limit the scope of the one or more embodiments of the present disclosure.
Preparation Example 1: Preparation of composite tablet using rosuvastatin mixture portion prepared using different methods
[Preparation of
ezetimibe
wet granules]
Ezetimibe-wet granules were prepared by mixing ezetimibe with lactose hydrate, microcrystalline cellulose, sodium lauryl sulfate, and croscarmellose sodium, according to the compositions represented in Table 1. The resulting mixture was put into a fluid-bed granulator, and then a binder solution (a solution of 4 mg of povidone dissolved in 90 mg of water) was injected thereinto by spraying while working the fluid-bed granulator at an inlet temperature of about 50℃, a spray pressure of about 1.0 bar, and a spray speed of about 10 rpm to granulate the mixture. The working conditions of the fluid-bed granulator were maintained constant while the binder solution was injected. After the granulation, the inlet temperature was lowered to about 45℃, and the resulting granules were dried at the reduced inlet temperature and sieved through a sieve having a mesh size of about 20, thereby preparing ezetimibe wet-granules.
Example
1: Preparation
of simple
rosuvastatin
mixture and composite tablet
The prepared ezetimibe wet granules as an ezetimibe wet granule portion were mixed with a simple mixture of additives for a rosuvastatin mixture portion as represented in Table 1. The resulting product was tableted with a tableting machine, thereby preparing composite tablets.
Example 2: Preparation of
rosuvastatin
-dry granule and composite tablet
A mixture of the additives for a rosuvastatin mixture portion as represented in Table 1 was sieved, mixed, dry-granulated with a roller compactor, and then broken down into dry granules, and sieved using an oscillator. The obtained rosuvastatin dry-granules were mixed with the ezetimibe wet granules prepared as described above, and the resulting mixture was then tableted using a tableting machine, thereby preparing composite tablets.
Comparative Example 1: Preparation of
rosuvastatin
wet-granules using high-speed mixer, and preparation of composite tablet
A mixture of the additives for a rosuvastatin mixture portion as represented in Table 1, excluding water, was mixed with 50 mg of water using a high-seed mixer under the following conditions: agitator speed of 150 rpm, agitator pressure of 1.6A, and chopper speed of 3,000 rpm, dried, and then sieved through a sieve having a mesh size of about 20, thereby preparing rosuvastatin wet granules. The obtained rosuvastatin wet-granules were mixed with the ezetimibe wet-granules prepared as described above, and the resulting mixture was then tableted using a tableting machine, thereby preparing composite tablets.
Comparative Example 2: Preparation of
rosuvastatin
wet-granules using fluid-bed granulator, and preparation of composite tablet
A mixture of the additives for a rosuvastatin mixture portion as represented in Table 1, excluding water, was added to a fluid-bed granulator, and then 50 mg of water was injected thereinto by spraying while working the fluid-bed granulator at an inlet temperature of about 50℃, a spray pressure of about 1.0 bar, and a spray speed of about 10 rpm, thereby granulating the mixture. The working conditions of the fluid-bed granulator were maintained constant while the water was injected. After the granulation, the inlet temperature was lowered to about 45℃, and the resulting granules were dried at the reduced inlet temperature and sieved through a sieve having a mesh size of about 20, thereby preparing rosuvastatin wet-granules. The resulting rosuvastatin wet granules were mixed with the ezetimibe wet granules prepared as described above, and the resulting mixture was then tableted using a tableting machine, thereby preparing composite tablets.
Preparation of composite tablets using different methods of forming rosuvastatin mixture portion
Example |
Preparation method of rosuvastatin mixture portion |
Types and amounts of additives for rosuvastatin mixture portion (mg) |
Types and amounts of additives for ezetimibe wet granule portion (mg) |
Example 1 |
Simple mixture(simple mixing) |
Rosuvastatin calcium(20 as Rosuvastatin)Microcrystalline cellulose 50Lactose hydrate 100Mannitol 100Crospovidone 3Magnesium stearate 4 |
Ezetimibe 10Lactose hydrate 30Microcrystalline cellulose 90Sodium lauryl sulfate 2Povidone 4Croscarmellose sodium 10Water (90) |
Example 2 |
Dry granules (Compacting) |
Comparative Example 1 |
Wet granules (High-speed mixer) |
Rosuvastatin calcium(Rosuvastatin 20)Microcrystalline cellulose 50Lactose hydrate 100Mannitol 100Crospovidone 3Magnesium stearate 4Water (50) |
Comparative Example 2 |
Wet granules(Fluid-bed granulator) |
(Microcrystalline cellulose in the rosuvastatin mixture portion: Comprecel M101D+)
(Povidone in the ezetimibe wet granule portion: PVP K-30 (weight average molecular weight: 30,000))
Preparation Example
2: Preparation
of composite tablet using rosuvastatin mixture portion including different
diluents
having different particle sizes
Composite tablets were prepared in the same manner as in Example 1, except that, as represented in Table 2, different types of microcrystalline cellulose having different particle sizes were used as diluents for rosuvastatin mixture portions, respectively. After ezetimibe wet granules were prepared in the same manner as in Example 1, the ezetimibe wet granules were mixed with a simple rosuvastatin mixture, and the resulting mixture was then tableted, thereby preparing composite tablets (Examples 3 and 4 and Comparative Examples 3 and 4).
Example |
Types of microcrystalline cellulose in rosuvastatin mixture portion |
d50(μm) |
d90(μm) |
Types and amounts of additives for ezetimibe wet granule portion (mg) |
Types and amounts of additives for rosuvastatin mixture portion (mg) |
Comparative Example 3 |
Avicel PH105 |
23 |
50 |
Ezetimibe 10Lactose hydrate 30Microcrystalline cellulose 90Sodium lauryl sulfate 2Povidone 4Croscarmellose sodium 10Water (90) |
Rosuvastatin calcium (20 as Rosuvastatin)Microcrystalline cellulose 50Lactose hydrate 100Mannitol 100Crospovidone 3Magnesium stearate 4 |
Example 1 |
Comprecel M101D+ |
44 |
98 |
Example 3 |
Avicel PH101 |
53 |
116 |
Example 4 |
Avicel PH102 |
108 |
229 |
Comparative Example 4 |
Vivapur 12 |
195 |
379 |
Povidone in the ezetimibe wet granule portion: PVP K-30 (weight average molecular weight: 30,000))
d50= median diameter of particle distribution
Preparation Example 3: Preparation of composite tablet using
povidones
having different weight average molecular weights in
ezetimibe
wet granule
Composite tablets were prepared in the same manner as in Example 1, except that, as represented in Table 3, povidones having different weight average molecular weights were used as binders for ezetimibe wet granules, respectively. After the ezetimibe wet granules were prepared in the same manner as in Example 1, the ezetimibe wet granules were mixed with each simple rosuvastatin mixture, and the resulting mixture was then tableted, thereby preparing composite tablets(Example 5 and Comparative Examples 5, 6, and 7).
Example |
Method of forming rosuvastatin mixture portion |
Weight average molecular weight of binder (povidone) |
Types and amounts of additives for ezetimibe wet granule portion (mg) |
Types and amounts of additives for rosuvastatin mixture portion (mg) |
Comparative Example 5 |
Simple mixing |
10,000 |
Ezetimibe 10Lactose hydrate 30Microcrystalline cellulose 90Sodium lauryl sulfate 2Povidone 4Croscarmellose sodium 10Water (90) |
Rosuvastatin calcium (20 as Rosuvastatin)Microcrystalline cellulose 50Lactose hydrate 100Mannitol 100Crospovidone 3Magnesium stearate 4 |
Example 1 |
30,000 |
Example 5 |
50,000 |
Comparative Example 6 |
400,000 |
Comparative Example 7 |
1,000,000 |
Test Example 1: Physical property measurement of mixture of
ezetimibe
wet granules and
rosuvastatin
simple mixture
Densities and angles of repose of the final mixtures of the ezetimibe wet granules and the rosuvastatin simple mixtures, obtained in Preparation Example 2, were measured, and the Hausner ratios (as a ratio of tapped density to bulk density) of the final mixtures were calculated. The results are shown in Table 4. For reference, an angle of repose at which productivity in tableting is ensured may be about 35° to about 40°.
|
Comparative Example 3 |
Example 1 |
Example 3 |
Example 4 |
Comparative Example 4 |
Density (g/mL) |
Bulk |
0.40 |
0.38 |
0.39 |
0.37 |
0.32 |
Tapped |
0.56 |
0.47 |
0.46 |
0.44 |
0.37 |
Hausner ratio |
1.40 |
1.24 |
1.18 |
1.19 |
1.16 |
Poor |
Fair |
Good |
Fair |
Good |
Angle of repose(°) |
46 |
38 |
38 |
37 |
32 |
Densities and angles of repose of final mixtures of the ezetimibe wet granules and the rosuvastatin simple mixtures with respect to the weight average molecular weights of the povidone in the ezetimibe wet granules, obtained in Preparation Example 2, were measured, and the Hausner ratios (as a ratio of tapped density to bulk density) of the final mixtures were calculated. The results are shown in Table 5.
Physical properties of ezetimibe wet granules with respect to weight average molecular weights of povidones used in ezetimibe wet granules
|
Comparative Example 5 |
Example 1 |
Example 5 |
Comparative Example 6 |
Comparative Example 7 |
Density (g/mL) |
Bulk |
0.42 |
0.38 |
0.37 |
0.36 |
0.37 |
Tapped |
0.57 |
0.46 |
0.44 |
0.41 |
0.41 |
Hausner ratio |
1.36 |
1.21 |
1.19 |
1.14 |
1.11 |
Poor |
Fair |
Fair |
Good |
Good |
Angle of repose (°) |
42 |
36 |
38 |
32 |
33 |
Test Example 2: Dissolution test (1)
A dissolution test was performed on the composite tablet of Example 1; the composite tablets of Examples 3 and 4 and Comparative Examples 3 and 4, obtained in Preparation Example 2; and the composite tablets of Examples 1, 5 and Comparative Examples 5, 6, and 7, obtained in Preparation Example 3, under the following conditions.
< Dissolution test conditions>
1) Dissolution method: Dissolution method II (paddle method) according to the Korean Pharmacopoeia
2) Dissolution medium: pH 4.5 sodium acetate buffer + 0.45 % SLS
3) Amount of dissolution medium: 500 mL
4) Temperature of dissolution apparatus: 37.5℃
5) Paddle speed: 75 rpm
6) Number of test sample per each experimental group: 6
7) Sampling time: 5 min and 30 min
< Sample analysis conditions >
Detector: UV-absorption detector (Measurement wavelength: 250 nm)
Column: ODS-2, C18, 150-mm column
Flow rate: 1.5 mL/min
Column temperature: 40℃
Analysis time: 20 min
< Test results >
Dissolution rate comparison of composite tablets with respect to particle size of diluents used in rosuvastatin mixture portion
Example |
5-min dissolution rate (%) |
30-min dissolution rate (%) |
Average |
Standard deviation |
Average |
Standard deviation |
Comparative Example 3 |
63.2 |
7.4 |
93.7 |
5.1 |
Example 1 |
61.5 |
4.8 |
93.5 |
3.3 |
Example 3 |
59.7 |
5.2 |
92.9 |
3.1 |
Example 4 |
57.0 |
8.6 |
93.1 |
5.3 |
Comparative Example 4 |
56.6 |
15.1 |
92.7 |
12.8 |
Dissolution rate comparison of composite tablets with respect to weight average molecular weights of povidones used in ezetimibe wet granules
Example |
5-min dissolution rate (%) |
30-min dissolution rate (%) |
Average |
Standard deviation |
Average |
Standard deviation |
Control formulation: Ezetrol® Tablet (available from MSD) |
56.2 |
5.2 |
90.6 |
3.4 |
Comparative Example 5 |
68.2 |
3.7 |
98.1 |
2.4 |
Example 1 |
61.5 |
4.8 |
93.5 |
3.3 |
Example 5 |
58.3 |
5.1 |
91.0 |
3.3 |
Example 6 |
55.1 |
4.2 |
89.7 |
3.7 |
Comparative Example 7 |
51.0 |
4.4 |
85.1 |
4.2 |
Test Example 3: Dissolution test (2)
A dissolution test was performed on the composite tablet of Example 1, and the composite tablets of Examples 3 and 4 and Comparative Examples 3 and 4, obtained in Preparation Example 2, under the following conditions.
< Dissolution test conditions >
1) Test method: Dissolution method II (paddle method) according to the Korean Pharmacopoeia
2) Dissolution medium: Simulated gastric fluid (SGF), prepared by dissolving 2.0 g of sodium chloride and 3.2 g of purified pepsin in 1,000 mL of water containing 7.0 mL of hydrochloric acid.
3) Amount of dissolution medium: 900 mL
4) Temperature of dissolution apparatus: 37.5℃
5) Paddle speed: 50 rpm
6) Number of test sample per each experimental group: 6
7) Sampling time: 5 min and 30 min
< Sample analysis conditions>
Detector: UV-absorption detector (Measurement wavelength: 250 nm)
Column: ODS-2, C18, 150-mm column
Flow rate: 1.5 mL/min
Column temperature: 40℃
Analysis time: 20 min
< Test results >
Dissolution rate comparison of composite tablets with respect to particle size of diluent used in rosuvastatin mixture portion
Example |
5-min dissolution rate (%) |
30-min dissolution rate (%) |
Average |
Standard deviation |
Average |
Standard deviation |
Comparative Example 3 |
4.7 |
1.1 |
29.7 |
1.8 |
Example 1 |
3.9 |
0.8 |
25.5 |
1.8 |
Example 3 |
3.6 |
1.4 |
26.1 |
2.1 |
Example 4 |
2.7 |
1.2 |
23.5 |
2.6 |
Comparative Example 4 |
1.6 |
1.2 |
15.3 |
3.2 |
Referring to Tables 4 and 6, the composite tablets of Comparative Example 3, including a water-insoluble diluent having a relatively small particle size in the rosuvastatin mixture portion, had an improved dissolution rate, but poor flowability due to a relatively large angle of repose. Flowability affects tableting properties of granules, and low flowability may lead to a large deviation in mass among tablets and low production efficiency, which would thereby render such mixtures unsuitable for mass production. The composite tablets of Comparative Example 4, including a water-insoluble diluent having a relatively large particle size in the rosuvastatin mixture portion, were found to have good flowability but a large deviation in dissolution rate. This is attributed to the water-insoluble diluent having a relatively large particle size in the rosuvastatin mixture portion being deposited in a dissolution vessel during the dissolution test, inhibiting the release of the active ingredient ezetimibe. The US Pharmacopeia (USP) prescribes that an acceptable deviation range of dissolution rates in the dissolution test is within 20% in 10 minutes or within 10% after 10 minutes (refer to USP 1092). Referring to Table 6, quality of the composite tablets of Comparative Example 4 could not be appropriately evaluated due to a large standard deviation in dissolution rate.
Referring to Tables 4 and 8, the composite tablets of Comparative Example 4 were found to exhibit a profile of delayed dissolution in simulated gastric fluid, relative to the composite tablets of the other examples. For ezetimibe having a Tmax of less than 1 hour, the initial dissolution rate, i.e., the release of ezetimibe in gastric juice, may have a major influence on the bioavailability of ezetimibe. Accordingly, the composite tablets of Comparative Example 4 are expected to have a low bioavailability in practice due to their low initial dissolution rate.
Referring to the results of Tables 5 and 7, the ezetimibe wet granules of Comparative Examples 6 and 7 prepared using povidones having large average molecular weights as binders had good flowability, while the composite tablets of Comparative Examples 6 and 7 including the ezetimibe wet granules were found to have lower initial dissolution rates, relative to the control formulation, due to the delayed dissolution of the ezetimibe according to the dissolution test. In consideration of the importance of the initial resolution rate of ezetimibe with respect to bioavailability, using povidones having large weight average molecular weights to prepare ezetimibe-wet granules as in Comparative Examples 6 and 7 was found to be undesirable in terms of dissolution rate of ezetimibe. On the contrary, the composite tablets of Comparative Example 5 prepared using povidone having a relatively low weight average molecular weight were found to have an improved initial ezetimibe resolution rate, but poor flowability of the ezetimibe wet granules and consequently lower tablet productivity, and thus are unsuitable in terms of productivity.
Test Example 4: Related compound test
After the composite tablets prepared in Examples 1 and 2 and Comparative Examples 1 and 2 were wrapped in aluminum-aluminum (Alu-Alu) blister packages and subjected to stressed conditions (about 60℃) for 2 weeks, lactone-related compounds were analyzed under the following conditions. The results are shown in Table 9.
<Test conditions>
< Sample analysis conditions>
Detector: UV-absorption detector (Measurement wavelength: 250 nm)
Column: ODS-2, C18, 150-mm column
Flow rate: 1.0 mL/min
Column temperature: 45℃
Analysis time: 70 min
Generation of lactone-related compounds of the composite tablets according to methods of preparing rosuvastatin mixture portion
Example |
Initial |
60℃ for 2 weeks(Alu-Alu blister package) |
Example 1 |
0.03 |
0.26 |
Example 2 |
0.04 |
0.29 |
Comparative Example 1 |
0.04 |
2.44 |
Comparative Example 2 |
0.03 |
1.97 |
Referring to the test results of Table 9, the composite tablets of Comparative Examples 1 and 2 including rosuvastatin wet granules, were found to include markedly increased lactone-related compounds after being left for 2 weeks for the test. Therefore, it was found that composite tablets having improved stability of rosuvastatin may be obtained by preparing the rosuvastatin mixture portion as a simple mixture or dry granules without using water.
It should be understood that the exemplary embodiments described therein should be considered in a descriptive sense only and not for purposes of limitation. Descriptions of features or aspects within each embodiment should typically be considered as available for other similar features or aspects in other embodiments. While one or more embodiments of the present invention have been described with reference to the figures, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the following claims.