WO2018077120A1 - 一种组合式离心装置 - Google Patents

一种组合式离心装置 Download PDF

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Publication number
WO2018077120A1
WO2018077120A1 PCT/CN2017/107091 CN2017107091W WO2018077120A1 WO 2018077120 A1 WO2018077120 A1 WO 2018077120A1 CN 2017107091 W CN2017107091 W CN 2017107091W WO 2018077120 A1 WO2018077120 A1 WO 2018077120A1
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Prior art keywords
negative pressure
storage chamber
thin tube
pressure storage
centrifugal device
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PCT/CN2017/107091
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English (en)
French (fr)
Inventor
尤剑琴
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深圳市孔雀生物科技有限公司
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Application filed by 深圳市孔雀生物科技有限公司 filed Critical 深圳市孔雀生物科技有限公司
Priority to US16/344,723 priority Critical patent/US20190271682A1/en
Priority to EP17865582.5A priority patent/EP3530354A4/en
Priority to JP2019521814A priority patent/JP7117014B2/ja
Publication of WO2018077120A1 publication Critical patent/WO2018077120A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • A61J1/20—Arrangements for transferring or mixing fluids, e.g. from vial to syringe
    • A61J1/2096—Combination of a vial and a syringe for transferring or mixing their contents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • A61J1/1462—Containers with provisions for hanging, e.g. integral adaptations of the container
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • A61J1/20—Arrangements for transferring or mixing fluids, e.g. from vial to syringe
    • A61J1/2003—Accessories used in combination with means for transfer or mixing of fluids, e.g. for activating fluid flow, separating fluids, filtering fluid or venting
    • A61J1/202—Separating means
    • A61J1/2034—Separating means having separation clips
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the invention relates to the technical field of medical technology, in particular to a combined centrifugal device suitable for layering cellular components.
  • Centrifugation technology is widely used in cell biology, molecular biology and biochemistry and other related fields. Under the action of centrifugal force, cells with different specific gravities in the suspension were layered and then separated and concentrated.
  • centrifuge tubes are all perfusion type, that is, the suspension to be layered is injected into the centrifuge tube from the top to the bottom, and the upper diameter of the centrifuge tube is greater than or equal to the diameter of the middle and bottom.
  • peripheral blood anticoagulated peripheral blood is composed of blood cells and plasma, and blood cells mainly include red blood cells, granulocytes, and mononuclear cells. Because of the different proportions of blood cells, they will be distributed at different levels of the centrifuge tube after natural sedimentation or centrifugation. Peripheral blood mononuclear cells account for only about one-thousandth of the blood cell components.
  • the red blood cells and granulocytes are located in the lower layer of the centrifuge tube, and the plasma is located in the upper layer of the centrifuge tube.
  • the mononuclear cells form a thin layer in the middle layer of the centrifuge tube, and it is very difficult to absorb because of the rare content.
  • the technical problem to be solved by the present invention is to provide a combined centrifugal device capable of efficiently separating a rare content of a suspension.
  • a technical solution adopted by the present invention is to provide a combined centrifugal device comprising: a thin tube; a negative pressure storage chamber for generating a negative pressure and storing a suspension, wherein the negative pressure storage chamber and the thinner When the tubes are connected so that the negative pressure storage chamber generates a negative pressure, The suspension is sucked into the negative pressure storage chamber and the thin tube through a thin tube; wherein the thin tube is provided with a fixing device for fixing the combined centrifugal device to the centrifuge through the fixing device, so that the combined centrifugal device is passed through the centrifuge The suspension in the suspension is centrifuged.
  • the fixing device is a buckle mechanism
  • the buckle mechanism is used for clamping on the centrifuge to fix the combined centrifugal device on the centrifuge.
  • the fixing device is a centrifugal cover connected to the centrifuge tube, and the thin tube is inserted in the centrifugal cover so that the combined centrifugal device is fixed in the centrifuge tube.
  • the negative pressure storage chamber comprises an elastic container to generate a negative pressure when the elastic container is squeezed.
  • the negative pressure storage chamber comprises at least two elastic containers communicating with each other to generate a negative pressure by squeezing one of the at least two interconnected elastic containers.
  • the combined centrifugal device further comprises a clamping member for clamping between at least two mutually communicating elastic containers.
  • the clamping member comprises a pinch valve.
  • the negative pressure storage chamber is provided with an opening for connecting with the negative pressure generator to generate a negative pressure by the negative pressure generator, wherein the negative pressure generator is an injection type syringe or a vacuum pump.
  • the inner diameter of the thin tube is less than or equal to 2 mm, and the width cross-sectional area of the negative pressure storage chamber is 3-20 times the width cross-sectional area of the thin tube.
  • the thin tube is transparent or translucent
  • the negative pressure storage chamber is transparent or translucent
  • the thin tube is integrally formed with the negative pressure storage chamber.
  • the utility model has the beneficial effects that the combined centrifugal device disclosed by the present invention comprises: a thin tube; a negative pressure storage chamber for generating a negative pressure and storing a suspension, wherein the negative pressure storage chamber is different from the prior art.
  • the thin tube is connected such that when the negative pressure storage chamber generates a negative pressure, the suspension is sucked into the negative pressure storage chamber and the thin tube through the thin tube; wherein the thin tube is provided with a fixing device for the combined centrifugal device through the fixing device Fixed on the centrifuge.
  • the present invention can directly fix the combined centrifugal device on the centrifuge through the fixing device, so that the centrifuge can store the rare content in the suspension by using the thin tube during the centrifugation process, and directly enlarge the rare content in the suspension.
  • the thickness of the ingredients in the thin tube In order to better separate the rare content of the suspension, the efficiency is greatly improved, and the layering and separation effects are better.
  • Figure 1 is a first schematic view showing the combined centrifugal device of the present invention
  • FIG. 2 is a schematic view showing the connection structure of the combined centrifugal device and the injection syringe of the present invention
  • Figure 3 is a schematic view showing the second structure of the combined centrifugal device of the present invention.
  • Figure 4 is a schematic view showing the third structure of the combined centrifugal device of the present invention.
  • Fig. 5 is a schematic view showing the connection structure of the combined centrifugal device and the centrifuge tube of the present invention.
  • Fig. 1 is a schematic view showing the first structure of the combined centrifugal device of the present invention.
  • the combined centrifugal device includes a thin tube 10, a negative pressure storage chamber 20, and a fixture 30.
  • the straw 10 is used to inhale the suspension.
  • the suspension may specifically be blood, peripheral blood or other body mixed suspension components.
  • the inner diameter of the thin tube 10 is less than or equal to 2 mm, and since the inner diameter of the thin tube 10 is less than or equal to 2 mm, the rare component is thickened in the thin tube, which is more advantageous for delamination and separation.
  • the suspension due to the presence of particles in the suspension, and the addition of the suspension has a certain viscosity, when the inner diameter of the thin tube 10 is less than or equal to 2 mm, the suspension will stay in the thin tube 10 without any pressure. The suspension is sealed to the capillary tube 10.
  • the negative pressure storage chamber 20 is for storing a suspension in which the negative pressure storage chamber 20 is in communication with the capillary tube 10.
  • the negative pressure storage chamber 20 is capable of generating a negative pressure so that when the negative pressure storage chamber 20 generates a negative pressure, the suspension is sucked into the negative pressure storage chamber 20 and/or the thin tube 10 through the thin tube 10.
  • the negative pressure storage chamber 20 includes an elastic container to generate a negative pressure when the elastic container 20 is squeezed. It should be understood that the present invention does not limit the shape of the elastic container.
  • the elastic container may specifically be a spherical shape, a cylindrical shape, a rectangular shape, an elliptical shape or other irregularly shaped elastic container.
  • the negative pressure storage chamber 20 includes an elastic container made of an elastic material, that is, a bomb The sexual container will return to its original shape when it is squeezed.
  • the thin tube 10 is placed in the suspension, and the user squeezes the elastic container by hand, first discharging the residual air inside the elastic container, so that a negative pressure is formed inside the elastic container, so that the suspension can be sucked into the elastic through the thin tube 10.
  • a small amount of air bubbles may exist. Since the negative pressure storage chamber 20 is an elastic container, the residual air bubbles can be completely discharged by shaking and squeezing the elastic container and sucking the suspension, and repeatedly operating.
  • the negative pressure storage chamber 20 includes at least two resilient containers that are in communication with each other to create a negative pressure by squeezing one of the at least two interconnected resilient containers.
  • the combined centrifugal device further includes a clamping member 20a for sandwiching between at least two mutually communicating elastic containers, wherein the clamping member 20a may specifically include a pinch valve, of course, this embodiment It is not limited that the clamping member 20a includes a pinch valve, and in other embodiments, the clamping member 20a may also include other clamping members. It should be understood that the invention does not limit the shape and number of at least two interconnected resilient containers. Specifically, as shown in FIG.
  • At least two mutually communicating elastic containers include a first spherical elastic container 201 and a second spherical elastic container 202 connected thereto, and the clamping member 20a is sandwiched by the first spherical shape. Between the elastic container 201 and the second spherical shaped elastic container 202. Of course, the first spherical-shaped elastic container 201 and the second spherical-shaped elastic container 202 are communicated by a small thin tube, and the clamping member 20a is sandwiched between the small thin tubes. Alternatively, as shown in FIG.
  • At least two mutually communicating elastic containers include a first elliptical elastic container 203, a second elliptical elastic container 204 in communication with the first elliptical elastic container 203, and a second
  • the elliptical elastic container 204 communicates with the third elliptical elastic container 205
  • the clamping member 20a is interposed between the first elliptical elastic container 203, the second elliptical elastic container 204, and the third elliptical elastic container 205.
  • the clamping member 20a is between the second elliptical elastic container 204 and the third elliptical elastic container 205.
  • first elliptical elastic container 203, the second elliptical elastic container 204, and the third elliptical elastic container 205 are communicated by a small thin tube, and the clamping member 20a is interposed on the small thin tube.
  • the at least two interconnected elastic containers are each an elastic container made of an elastic material, that is, deformed to generate a negative pressure when pressed.
  • the user squeezes one of the at least two interconnected elastic containers by hand, such that the residual air inside the at least two interconnected elastic containers is first discharged, so that at least two interconnected elastic containers are connected.
  • a negative pressure is formed inside so that the suspension can be sucked through the capillary 10 into at least two interconnected elastic containers.
  • a small amount of air remains inside the negative pressure storage chamber 20, so that by providing at least two mutually communicating elastic containers, the clamping member 20a is clamped to the two.
  • the lower ones can all be used to store the suspension, ensuring that no bubbles are present in the suspension.
  • the negative pressure storage chamber 20 is provided with an opening for connecting with the negative pressure generator to generate a negative pressure by the negative pressure generator. It should be understood that the present embodiment does not limit the position at which the negative pressure storage chamber 20 is provided with an opening, which may be disposed at the bottom or side of the negative pressure storage chamber 20 or the like.
  • the negative pressure generator may specifically be an injection type syringe 50 or a vacuum pump to generate a negative pressure through the injection type syringe 50 or a vacuum pump. It is worth noting that the inner diameter of the opening is less than or equal to 2 mm so that the suspension is drawn into the rigid container through the syringe 50 or vacuum pump.
  • the negative pressure storage chamber 20 may actually be a rigid container having an opening for connecting with the negative pressure generator to generate a negative pressure by the negative pressure generator, and hard A sealing cover may also be provided on the container for sealing the opening.
  • the thin tube 10 is transparent or translucent, that is, the thin tube 10 is made of a transparent or translucent material, so that the suspension can be seen inside the thin tube 10, that is, the rare component in the suspension is in the thin tube. 10 in thickness.
  • the thin tube 10 can also be provided with a scale, and the thickness of the suspension in the thin tube 10 can be read by the scale.
  • the negative pressure storage chamber 20 is transparent or translucent, that is, the negative pressure storage chamber 20 is made of a transparent material, so that the suspension can be seen inside the negative pressure storage chamber 20, that is, the suspension takes up
  • the thickness of the storage chamber 20 is pressurized.
  • the negative pressure storage chamber 20 can also be provided with a scale through which the thickness of the suspension in the negative pressure storage chamber 20 can be read.
  • the thin tube 10 and the negative pressure storage chamber 20 are integrally formed, and the structure is simple, and the design cost can be reduced.
  • the thin tube 10 is detachably disposed on the negative pressure storage chamber 20, specifically, the thin tube 10 is screwed to the negative pressure storage chamber 20, that is, the thin tube 10 is provided with an external thread, and the negative pressure storage chamber 20 is provided with an internal thread, and the external thread is screwed to the internal thread, so that the thin tube 10 and the negative pressure storage chamber 20 can be detachably connected.
  • the inner diameters of the thin tubes 10 are all equal.
  • the inner diameter of the thin tube 10 changes gradually, that is, the inner diameter of the thin tube 10 gradually decreases from the end connected to the negative pressure storage chamber 20 to the other end away from the negative pressure storage chamber 20. This makes it possible to enlarge the minute rare content in the thin tube 10.
  • the inner diameter of the thin tube 10 is arcuately connected to the inner diameter of the negative pressure storage chamber 20, so that the rare content of the suspension can be quickly separated into the thin tube 10, preferably the thin tube 10 and the negative tube.
  • the angle of the arcuate connection at the junction of the pressure storage chamber 20 is 30-60 degrees.
  • the capillary tube 10 is provided with a fixing device 30 for fixing the combined centrifugal device to the centrifuge through the fixing device 30, so that the suspension in the combined centrifugal device is centrifuged by the centrifuge, that is, the centrifuge can be used
  • the rare content of the suspension in the negative pressure storage chamber 20 is separated in the thin tube 10, and the suspension is directly centrifuged through a simple combined centrifugal device, which greatly reduces the operation time and design cost.
  • the inner diameter of the negative pressure storage chamber 20 is equal, and the width cross-sectional area of the negative pressure storage chamber 20 is 3-20 times the width cross-sectional area of the thin tube 10, specifically, the negative pressure storage chamber 20
  • the width cross-sectional area is 10 times the width cross-sectional area of the thin tube 10 (that is, in the case where the inner diameter of the thin tube 10 is equal), so that as long as the suspension of the negative pressure storage chamber 20 contains a rare content, It is enlarged and displayed in the thin tube 10, so that the components in the thin tube 10 can be directly sucked out or the components in the thin tube 10 can be taken out by cutting off the thin tube 10, which makes it easier to separate the rare content in the suspension. Ingredients.
  • the length H1 of the thin tube 10 is 10-20 times the length of the negative pressure storage chamber 20.
  • the inner diameters of the negative pressure storage chambers 20 may also be unequal, that is, the shape of the negative pressure storage chamber 20 is spherical, gourd shape or irregular shape.
  • the fixing device 30 is integrally formed with the thin tube 10.
  • the fixture 30 is detachably disposed on the tubule 10.
  • the fixing device 30 is a snap mechanism, and the snap mechanism 30 is used for clamping on the centrifuge to fix the combined centrifugal device on the centrifuge.
  • the buckle mechanism 30 is fixed in the thin tube 10, and the buckle mechanism 30 is provided with a protrusion.
  • the panel or the plug of the centrifuge is provided with a groove, and the protrusion is placed in the groove and the gap between the protrusion and the groove Cooperating, the combined centrifuge device is placed on the centrifuge.
  • the fixing device 30 is a centrifugal cover connected to the centrifuge tube 40, and the thin tube 10 is inserted in the centrifugal cover so that the combined centrifugal device is fixed in the centrifuge tube 40, thereby passing
  • the centrifuge tube 40 holds the combined centrifuge device on the centrifuge.
  • the thin tube 10 is detachably disposed in the centrifugal cover. Specifically, the centrifugal cover is provided with an opening, and the thin tube 10 is inserted in the opening.
  • the outer diameter of the thin tube 10 gradually decreases from the end connected to the negative pressure storage chamber 20 to the other end away from the negative pressure storage chamber 20.
  • the centrifugation is performed at this time.
  • the cover is placed in the thin tube 10, and the thin tube 10 can be fixed in the centrifuge tube by a centrifugal cover.
  • the tubule 10 is integrally formed with the centrifuge cap.
  • the length of the capillary 10 and the negative pressure storage chamber 20 is longer than the length of the centrifuge tube 20, that is, when the combined centrifugal device is fixed in the centrifuge tube 40, the negative pressure storage chamber 20 is disposed at the bottom of the centrifuge tube 40 to pass
  • the bottom of the centrifuge tube 40 supports the negative pressure storage chamber 20 so that the negative pressure storage chamber 20 does not move during centrifugation; in addition, when the combined centrifugal device is fixed in the centrifuge tube 40, the end of the thin tube 10 is more than the fixed device
  • the level of 30 is high, so that even if the thin tube 10 is properly displaced under centrifugation during centrifugation, the effect of delamination and thickening is not affected.
  • the present embodiment absorbs the mixed suspension by generating a negative pressure mode, thereby avoiding the conventional and prior art operations of injecting the suspension into the centrifuge tube, reducing pollution and generating bubbles; meanwhile, the present invention is performed by combined centrifugation.
  • the device is provided with a long thin tube structure, which is centrifuged in the case of the same volume of suspension, especially when the volume is small or small. After centrifugation, the thin tube of the combined centrifugal device can well amplify the thickness of the rare content of the suspension in the negative pressure storage chamber, which facilitates better centrifugation of rare cellular components.
  • the combined centrifugal device disclosed in the present invention comprises: a thin tube; a negative pressure storage chamber for generating a negative pressure and storing a suspension, wherein the negative pressure storage chamber communicates with the thin tube to make the negative pressure storage chamber negative At the time of pressing, the suspension is sucked into the negative pressure storage chamber and the thin tube through a thin tube; wherein the thin tube is provided with a fixing device for fixing the combined centrifugal device to the centrifuge by the fixing device.
  • the present invention can directly fix the combined centrifugal device on the centrifuge through the fixing device, so that the centrifuge can store the rare content in the suspension by using the thin tube during the centrifugation process, and directly enlarge the rare content in the suspension.
  • the thickness of the ingredients in the thin tube facilitates better separation of the rare components of the suspension, greatly improving efficiency, stratification and separation.

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Abstract

一种组合式离心分离装置，包括：细管（10）；负压存储腔（20），用于产生负压并存储悬浮液，其中负压存储腔（20）与细管（10）连通，以使得负压存储腔（20）产生负压时，通过细管（10）将悬浮液吸入负压存储腔（20）和细管（10）内，其中细管（10）上设置有固定装置（30），以通过固定装置（30）将组合式离心装置固定在离心机上。

Description

一种组合式离心装置 技术领域
本发明涉及医疗技术领域，尤其涉及一种适用于细胞成份分层的组合式离心装置。
背景技术
离心技术广泛应用于细胞生物学、分子生物学和生物化学及其他相关领域。在离心力作用下，悬浮液中比重不同的细胞得以分层然后分离浓缩。
传统的离心管均为灌注式，即将待分层的悬浮液从上向下注入离心管，而离心管的上部直径大于或等于中间和底部的直径。
传统的离心操作中，悬浮液中不同比重的细胞离心后处于离心管的不同层面，一些稀有含量的细胞成分在传统离心管中形成的薄层非常薄，比较难吸取分离。以外周血为例，抗凝的外周血由血细胞和血浆组成，血细胞主要包括红细胞、粒细胞、单个核细胞。因为各类血细胞比重不同，经过自然沉降或离心，将分布在离心管的不同层面。外周血中单个核细胞占血细胞成分的比例仅仅只有千分之一左右。离心后红细胞和粒细胞位于离心管的下层，血浆位于离心管的上层，单个核细胞形成薄层位于离心管的中层，因为稀有含量，吸取非常困难。
发明内容
本发明主要解决的技术问题是提供一种组合式离心装置，能够有效地分离出悬浮液中稀有含量的成份。
为解决上述技术问题，本发明采用的一个技术方案是：提供一种组合式离心装置，包括：细管；负压存储腔，用于产生负压并存储悬浮液，其中负压存储腔与细管连通，以使得负压存储腔产生负压时， 通过细管将悬浮液吸入在负压存储腔和细管内；其中，细管上设置有固定装置，以通过固定装置将组合式离心装置固定在离心机上，以使得通过离心机对组合式离心装置中的悬浮液进行离心。
其中，固定装置为一卡扣机构，卡扣机构用于卡置在离心机上，以将组合式离心装置固定在离心机上。
其中，固定装置为与离心管连接的离心盖，细管插在离心盖中，以使得组合式离心装置固定在离心管中。
其中，负压存储腔包括弹性容器，以通过挤压弹性容器时产生负压。
其中，负压存储腔包括至少两个互相连通的弹性容器，以通过挤压至少两个互相连通的弹性容器中的一者时产生负压。
其中，组合式离心装置还包括夹紧部件，夹紧部件用于夹设在至少两个互相连通的弹性容器之间。
其中，夹紧部件包括夹管阀。
其中，负压存储腔上设有一开口，开口用于与负压产生器连接，以通过负压产生器产生负压，其中负压产生器为注射式针筒或真空泵。
其中，细管的内径小于或等于2毫米，负压存储腔的宽度横截面积是细管的宽度横截面积3-20倍。
其中，细管透明或半透明，负压存储腔透明或半透明，细管与负压存储腔一体成型。
本发明的有益效果是：区别于现有技术的情况，本发明所公开的组合式离心装置包括：细管；负压存储腔，用于产生负压并存储悬浮液，其中负压存储腔与细管连通，以使得负压存储腔产生负压时，通过细管将悬浮液吸入在负压存储腔和细管内；其中，细管上设置有固定装置，以通过固定装置将组合式离心装置固定在离心机上。通过上述方式，本发明可以直接通过固定装置将组合式离心装置固定在离心机上，使得离心机在离心过程中，能够利用细管存储悬浮液中稀有含量的成份，直接放大了悬浮液中稀有含量的成份在细管中的厚度，便 于更好分离出悬浮液中稀有含量的成份，大大提高了效率，分层和分离效果更好。
附图说明
图1是本发明组合式离心装置的第一结构示意图；
图2是本发明组合式离心装置与注射式针筒的连接结构示意图；
图3是本发明组合式离心装置的第二结构示意图；
图4是本发明组合式离心装置的第三结构示意图；
图5是本发明组合式离心装置和离心管的连接结构示意图。
具体实施方式
下面结合附图和实施方式对本发明进行详细说明。
如图1所示，图1是本发明组合式离心装置的第一结构示意图。该组合式离心装置包括细管10、负压存储腔20和固定装置30。
细管10用于吸入悬浮液。应理解，悬浮液具体可以是血液、外周血或其他人体混合悬浮成份。
在本实施例中，细管10的内径小于或等于2毫米，由于细管10的内径小于或等于2毫米，这样使稀有成分在细管中变厚，更加有利于分层和分离。另外，由于悬浮液中存在颗粒，且加上悬浮液具有一定的粘稠度，当细管10的内径小于或等于2毫米时，悬浮液会在没有任何压力的情况下停留在细管10内，使得悬浮液密封细管10。
负压存储腔20用于存储悬浮液，其中负压存储腔20与细管10连通。在本实施例中，负压存储腔20能够产生负压，以使得负压存储腔20产生负压时，通过细管10将悬浮液吸入在负压存储腔20和/或细管10内。
在本实施例中，负压存储腔20包括弹性容器，以通过挤压弹性容器20时产生负压。应理解，本发明并不限定弹性容器的形状，弹性容器具体可以为球形状、圆柱状、矩形状、椭圆形状或其他不规则形状的弹性容器。
具体地，负压存储腔20包括由弹性材料制成的弹性容器，即弹 性容器在受到挤压时会恢复原形。使用时，将细管10放置在悬浮液里，用户用手挤压弹性容器，先将弹性容器内部残余的空气排出，使得弹性容器内部形成负压，从而可以通过细管10将悬浮液吸入弹性容器和/或细管10内。另外，细管10吸取悬浮液后，会有少量气泡存在，由于负压存储腔20为弹性容器，可通过摇动和挤压弹性容器并吸取悬浮液，反复操作，可以完全排出残余的气泡。
当然，在一些实施例中，负压存储腔20包括至少两个互相连通的弹性容器，以通过挤压至少两个互相连通的弹性容器中的一者时产生负压。且组合式离心装置还包括夹紧部件20a，夹紧部件20a用于夹设在至少两个互相连通的弹性容器之间，其中夹紧部件20a具体可以包括夹管阀，当然，本实施例并不限定夹紧部件20a包括夹管阀，在其他实施例中，夹紧部件20a还可以包括其他夹紧部件。应理解，本发明并不限定至少两个互相连通的弹性容器的形状和数量。具体地，如图3所示，至少两个互相连通的弹性容器包括第一球形状的弹性容器201和与其连通的第二球形状的弹性容器202，夹紧部件20a夹设在第一球形状的弹性容器201和第二球形状的弹性容器202之间。当然，第一球形状的弹性容器201和第二球形状的弹性容器202之间通过一小细管连通，夹紧部件20a夹设在小细管上。又或者，如图4所示，至少两个互相连通的弹性容器包括第一椭圆状的弹性容器203、与第一椭圆状的弹性容器203连通的第二椭圆状的弹性容器204以及与第二椭圆状的弹性容器204连通的第三椭圆状的弹性容器205，夹紧部件20a夹设在第一椭圆状的弹性容器203、第二椭圆状的弹性容器204和第三椭圆状的弹性容器205之间，具体地，夹紧部件20a第二椭圆状的弹性容器204和第三椭圆状的弹性容器205之间。当然，第一椭圆状的弹性容器203、第二椭圆状的弹性容器204和第三椭圆状的弹性容器205之间通过一小细管连通，夹紧部件20a夹设在小细管上。
具体地，至少两个互相连通的弹性容器均为一由弹性材料制成的弹性容器，即在受到挤压时会变形产生负压。使用时，将细管10放 置在悬浮液里，用户用手挤压至少两个互相连通的弹性容器中的一者，这样先将至少两个互相连通的弹性容器内部残余的空气排出，使得至少两个互相连通的弹性容器内部形成负压，从而可以通过细管10将悬浮液吸入在至少两个互相连通的弹性容器内。另外，值得注意的是，在通过细管10吸入悬浮液时，负压存储腔20内部会残留少量空气，因此通过设置至少两个互相连通的弹性容器，利用夹紧部件20a夹紧在两个互相连通的弹性容器之间，从而可以使残留的少量空气被隔离在至少两个互相连通的弹性容器的一者中(即最远离细管10的一个)，至少两个互相连通的弹性容器剩下的可以全部用来存储悬浮液，保证悬浮液中不存在气泡。在本实施例中通过两个以上的弹性容器和夹紧部件20a可以很方便和快速的隔离气泡在弹性容器的尾部或者其他位置。当然，还可以结合多次摇动和多次反复挤压弹性容器来吸取悬浮液，进一步排出残余的气泡，多重方式排出气泡，保证了负压存储腔20内的悬浮液不存在气泡。
当然，在一些实施例中，如图2所示，负压存储腔20上设有一开口，开口用于与负压产生器连接，以通过负压产生器产生负压。应理解，本实施例并不限定负压存储腔20设置开口的位置，该开口可以设置在负压存储腔20的底部或、侧边或其他位置等。负压产生器具体可以为注射式针筒50或真空泵，以通过注射式针筒50或真空泵产生负压。值得注意的是，开口的内径小于或等于2毫米，这样在通过注射式针筒50或真空泵将悬浮液吸入硬质容器内。当然，还可以在负压存储腔20上设有密封盖，用于密封开口。更具体地，负压存储腔20实际上可以为一硬质容器，该硬质容器上设有一开口，开口用于与负压产生器连接，以通过负压产生器产生负压，且硬质容器上还可以设有密封盖，用于密封开口。
在本实施例中，细管10透明或半透明，即细管10由透明或半透明材料制成，从而可以看到悬浮液在细管10内部的情况，即悬浮液中稀有成分在细管10中的厚度。当然，细管10还可以设置有刻度，可通过刻度读出悬浮液在细管10的厚度。
在本实施例中，负压存储腔20透明或半透明，即负压存储腔20由透明材料制成，从而可以看到悬浮液在负压存储腔20内部的情况，即悬浮液所占负压存储腔20的厚度。当然，负压存储腔20还可以设置有刻度，可通过刻度读出悬浮液在负压存储腔20的厚度。
在本实施例中，细管10与负压存储腔20一体成型，结构简单，能够减低设计成本。当然，在其他实施例中，细管10可拆卸设置在负压存储腔20上，具体地，细管10与负压存储腔20螺纹连接，即细管10设有外螺纹，负压存储腔20设有内螺纹，外螺纹与内螺纹螺纹连接，从而可实现细管10与负压存储腔20可拆卸连接。
在本实施例中，细管10的内径均相等。当然，在其他实施例中，细管10的内径呈逐渐变小方式变化，即细管10的内径从与负压存储腔20连接的一端向远离负压存储腔20的另一端逐渐变小，这样能够将微小的稀有含量放大在细管10中。
在本实施例中，细管10的内径与负压存储腔20的内径弧形连接，这样能够快速地将悬浮液中稀有含量的成份分离到细管10中，优选地，细管10和负压存储腔20连接处的弧形连接的角度是30-60度。
另外，细管10上设置有固定装置30，以通过固定装置30将组合式离心装置固定在离心机上，以使得通过离心机对组合式离心装置中的悬浮液进行离心，即可以通过离心机将负压存储腔20的悬浮液中稀有含量的成份分离在细管10中，直接通过结构简单的组合式离心装置对悬浮液进行离心，大大减低操作时间和设计成本。
在本实施例中，负压存储腔20的内径相等，且负压存储腔20的宽度横截面积是细管10的宽度横截面积的3-20倍，具体地，负压存储腔20的宽度横截面积是细管10的宽度横截面积的10倍(即在细管10的内径相等的情况下)，这样只要负压存储腔20的悬浮液中含有稀少含量的成份，都能够将其放大显示在细管10中，这样可以直接将细管10中的成份吸出来或通过剪掉细管10再将细管10中的成份取出来，这样更方便分离出悬浮液中稀有含量的成份。当然，细管10的长度H1是负压存储腔20的长度的10-20倍。当然，在其他实 施例中，负压存储腔20的内径也可以不相等，即负压存储腔20的形状呈球形、葫芦形状或不规则形状。
在本实施例中，固定装置30与细管10一体成型。当然，在其他实施例中，固定装置30可拆卸设置在细管10上。
在本实施例中，固定装置30为一卡扣机构，卡扣机构30用于卡置在离心机上，以将组合式离心装置固定在离心机上。具体地，卡扣机构30固定在细管10中，且卡扣机构30设有凸起，离心机的面板或塞子设有凹槽，凸起卡置在凹槽中且凸起与凹槽间隙配合，使得组合式离心装置卡置在离心机上。
在一些实施例中，如图5所示，固定装置30为与离心管40连接的离心盖，细管10插设在离心盖中，以使得组合式离心装置固定在离心管40中，从而通过离心管40将组合式离心装置固定在离心机上。应理解，细管10可拆卸设置在离心盖中，具体地，离心盖设有一开孔，细管10插设在开孔中。另外，细管10的外径从与负压存储腔20连接的一端向远离负压存储腔20的另一端逐渐变小，当细管10的外径与开孔的内径相等时，此时离心盖卡置在细管10中，可以通过离心盖将细管10固定在离心管中。当然，在其他实施例中，细管10与离心盖一体成型。
应理解，细管10和负压存储腔20长度比离心管20的长度长，即当组合式离心装置固定在离心管40中时，负压存储腔20设置在离心管40的底部，以通过离心管40的底部支撑负压存储腔20，使得负压存储腔20在离心过程中不会移动；另外，当组合式离心装置固定在离心管40中时，细管10的端部比固定装置30的水平高度高，这样在离心时候，即使细管10在离心作用下有适当位移，也不影响分层和变厚的效果。
本实施例通过产生负压方式吸取混合悬浮液，从而避免了传统的和现有技术中将悬浮液灌注进入离心管的操作方式，减少了污染和产生气泡；同时，本发明通过在组合式离心装置设置长条细管型结构，在同样体积的悬浮液情况下，特别是体积量少量或微量时，经过离心 机离心后，组合式离心装置的细管可以很好地放大悬浮液中稀有含量的成份在负压存储腔中的厚度，便于更好离心分离稀有含量的细胞成份。
综上，本发明所公开的组合式离心装置包括：细管；负压存储腔，用于产生负压并存储悬浮液，其中负压存储腔与细管连通，以使得负压存储腔产生负压时，通过细管将悬浮液吸入在负压存储腔和细管内；其中，细管上设置有固定装置，以通过固定装置将组合式离心装置固定在离心机上。通过上述方式，本发明可以直接通过固定装置将组合式离心装置固定在离心机上，使得离心机在离心过程中，能够利用细管存储悬浮液中稀有含量的成份，直接放大了悬浮液中稀有含量的成份在细管中的厚度，便于更好分离出悬浮液中稀有含量的成份，大大提高了效率，分层和分离效果更好。
以上所述仅为本发明的实施方式，并非因此限制本发明的专利范围，凡是利用本发明说明书及附图内容所作的等效结构或等效流程变换，或直接或间接运用在其他相关的技术领域，均同理包括在本发明的专利保护范围内。

Claims (10)

  1. 一种组合式离心装置，其特征在于，包括：
    细管；
    负压存储腔，用于产生负压并存储悬浮液，其中所述负压存储腔与所述细管连通，以使得所述负压存储腔产生负压时，通过所述细管将所述悬浮液吸入在所述负压存储腔和细管内；
    其中，所述细管上设置有固定装置，以通过所述固定装置将所述组合式离心装置固定在离心机上，以使得通过所述离心机对所述组合式离心装置中的所述悬浮液进行离心。
  2. 根据权利要求1所述的组合式离心装置，其特征在于，所述固定装置为一卡扣机构，所述卡扣机构用于卡置在所述离心机上，以将所述组合式离心装置固定在所述离心机上。
  3. 根据权利要求1所述的组合式离心装置，其特征在于，所述固定装置为与离心管连接的离心盖，所述细管插在所述离心盖中，以使得所述组合式离心装置固定在所述离心管中。
  4. 根据权利要求1所述的组合式离心装置，其特征在于，所述负压存储腔包括弹性容器，以通过挤压所述弹性容器时产生负压。
  5. 根据权利要求4所述的组合式离心装置，其特征在于，所述负压存储腔包括至少两个互相连通的弹性容器，以通过挤压所述至少两个互相连通的弹性容器中的一者时产生负压。
  6. 根据权利要求5所述的组合式离心装置，其特征在于，所述组合式离心装置还包括夹紧部件，所述夹紧部件用于夹设在所述至少两个互相连通的弹性容器之间。
  7. 根据权利要求6所述的组合式离心装置，其特征在于，所述夹紧部件包括夹管阀。
  8. 根据权利要求1所述的组合式离心装置，其特征在于，所述负压存储腔上设有一开口，所述开口用于与负压产生器连接，以通过所述负压产生器产生负压，其中所述负压产生器为注射式针筒或真空 泵。
  9. 根据权利要求1所述的组合式离心装置，其特征在于，所述细管的内径小于或等于2毫米，所述负压存储腔的宽度横截面积是所述细管的宽度横截面积3-20倍。
  10. 根据权利要求1所述的组合式离心装置，其特征在于，所述细管透明或半透明，所述负压存储腔透明或半透明，所述细管与所述负压存储腔一体成型。
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