WO2017214839A1 - Multi-functional reagent kit - Google Patents

Multi-functional reagent kit Download PDF

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Publication number
WO2017214839A1
WO2017214839A1 PCT/CN2016/085644 CN2016085644W WO2017214839A1 WO 2017214839 A1 WO2017214839 A1 WO 2017214839A1 CN 2016085644 W CN2016085644 W CN 2016085644W WO 2017214839 A1 WO2017214839 A1 WO 2017214839A1
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Prior art keywords
reagent
kit
kit according
multifunctional
control unit
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PCT/CN2016/085644
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French (fr)
Chinese (zh)
Inventor
彭鹏
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彭鹏
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Priority to PCT/CN2016/085644 priority Critical patent/WO2017214839A1/en
Publication of WO2017214839A1 publication Critical patent/WO2017214839A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
    • G16Z99/00—Subject matter not provided for in other main groups of this subclass

Definitions

  • the present invention relates to the field of biotechnology, and in particular to a multifunctional kit.
  • kits In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role.
  • the kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.
  • the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated.
  • the general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water.
  • the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples.
  • kits As the development of biology and the interdisciplinary processes of biology and other closely related disciplines increase, the variety of kits continues to increase. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions.
  • the present invention provides a multifunctional kit.
  • the technical solution adopted by the present invention is: a multifunctional kit comprising a kit body and a lid, the kit body is provided with a sounding device and a control unit, a small battery, a display device and a close range Wireless communication NFC tag.
  • the sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device and the temperature control probe And the control unit is electrically connected; the short-range wireless communication NFC tag is composed of a data storage module and a data transmission module, the output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the NFC chip to Intelligent management of the use of the staff
  • the inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two.
  • the lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.
  • the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.
  • the kit body has a two-layer structure, and the control unit, the sounding device and the small storage battery are disposed between the inner layer and the outer layer of the reagent cartridge body.
  • the number of the reagent wells is six.
  • the display device is a digital display screen.
  • the sounding device is a buzzer, and the number is two.
  • control unit is a single chip microcomputer.
  • the small battery is a lithium ion battery.
  • the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
  • the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the sensor.
  • the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.
  • the smart terminal used by the staff is set on the mobile phone and displays related information of the kit through the mobile phone interface.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device.
  • the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample.
  • the experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.
  • 2 is a structural diagram of the reagent well.
  • FIG. 3 is a schematic structural view of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.
  • a multifunctional kit includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 , a sounding device 40, and a control unit (not shown). ), a small battery (not shown), a display device 80, and a short-range wireless communication NFC tag 90.
  • the sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected.
  • the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
  • the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is further disposed therebetween, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36.
  • the sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32.
  • the rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated.
  • the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50.
  • the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32.
  • the color of the disk 36 is rotated and the arrow indicator light 34 is illuminated.
  • the color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36.
  • the eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively.
  • control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
  • the short-range wireless communication NFC tag 90 mainly includes a data storage module 901, a data transmission module 902, and an NFC chip 903.
  • the output of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal (usually the mobile phone 910) used by the user through the NFC chip.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once it exceeds the setting range, The sound device immediately alarms the experimenter to reduce the probability of the experiment failure; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.
  • the present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.

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Abstract

A multi-functional reagent kit. By providing a sound generating device (40), a control unit, and a storage battery at a main body (10) of the reagent kit, and providing a refrigerating device (381) and a temperature control probe (382) at an external wall of a reagent recess, the present invention provides a desired pre-cooling environment (2-8°C) for a reagent, and enables temperature detection by means of the temperature control probe (382), and display of the temperature on a display device (80) for lab personnel. When the temperature exceeds a preset range, the sound generating device (40) immediately gives an alarm to notify lab personnel, thus reducing the probability of experiment failures. At the same time, by employing a reagent turntable (36) to drive reagent recesses (38) to rotate, the present invention provides a loading reminder to lab personnel to prevent experiment failures caused by a loading error. In addition, by employing contactless end-to-end data transmission between an intelligent management terminal and the reagent kit within a five-centimeter range, the present invention solves numerous problems of manufacturing management methods of conventional reagent kits, thus improving efficiency and precision of management, and reducing a management cost of the manufacturer.

Description

发明名称：一种多功能的试剂盒  Title of Invention: A Multifunctional Kit
技术领域  Technical field
[0001] 本发明涉及生物技术领域， 特别是涉及一种多功能的试剂盒。  [0001] The present invention relates to the field of biotechnology, and in particular to a multifunctional kit.
背景技术  Background technique
[0002] 生物技术领域的发展， 除了技术上的突破外， 更重要的是通过研究和实验效率 的提高， 而这其中， 试剂盒的出现起到了很重要的作用。 试剂盒将常见的生物 学实验的步骤标准化， 为实验者提供将配制好的试剂和优化的加样方法， 大幅 提高了实验幵展的速度和结果的标准化程度。  [0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.
技术问题  technical problem
[0003] 由于生物学的特性， 很多试剂在使用吋为保证试剂的有效性和实验的准确性， 需要在低温 （2-8°C) 环境下完成， 而目前， 此类操作通常是使用"冰盒"来完成 ， 即将冰块加到大小合适的泡沫盒中， 然后将试剂管插到冰中， 确保试剂处于 所需的低温状态。 但由于很多生物学实验需要进行无菌操作， 而冰块很容易沾 上空气中的细菌， 是实验中细菌污染的一个潜在来源， 因此使用"冰盒"进行的低 温加样操作在做这些实验吋对实验人员的操作要求较高， 提高了实验人员的负 担， 并且实验成功率无法得到保证。 用"冰盒"的另一个缺点是， 无法确保插在其 中的试剂管是否为所需的温度环境 （2-8°C) 。 当温度超出这一范围， 实验人员 并无法立即得知这一情况， 可能也会对实验结果造成一定的影响。  [0003] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed. Another disadvantage of using an "ice box" is that it is not possible to ensure that the reagent tube inserted in it is in the desired temperature environment (2-8 ° C). When the temperature is outside this range, the experimenter is not immediately aware of the situation and may have a certain impact on the experimental results.
[0004] 另外， 由于实验的复杂程度日益增高， 现有试剂盒的试剂数也在不断增多， 加 样步骤更是日渐复杂化。 以常用的荧光定量 PCR试剂盒为例， 一般试剂盒内都带 有 Taq酶、 缓冲液、 显色染料和无菌超纯水 4种试剂， 另外需要实验者自备的试 剂至少包括上游引物、 下游引物和模板， 一共 7种试剂共同配制荧光定量 PCR的 反应体系。 很多吋候， 实验者在加样的过程中会出现漏加、 错加、 多加等现象 ， 究其原因， 就在于试剂种类繁多， 加样过程无法很好地进行识别和标记， 一 旦因需要临吋处理其他紧急事务而导致正在进行的实验被打断吋， 出错就在所 难免。 [0005] 随着生物学的发展以及生物学与其他联系紧密的学科交织形成交叉学科的增多 ， 试剂盒的种类也在不断地增多。 对于试剂盒的生产厂家， 尤其是生产种类繁 多、 应用广泛的试剂盒的大型厂家来说， 对于所生产的试剂盒的管理一直都是 非常具有挑战性的工作。 目前， 大部分生产商使用的管理方法还是由工作人员 将试剂盒的相关信息记录在纸质文档中， 并于需要吋找出相关文档进行核对。 这种通过人工记录和比对的管理模式导致了试剂盒管理的效率低下且出错率高 ， 而每年因此产生的用于"纠错"的额外支出高达数百万乃至数千万元。 [0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing ongoing experiments to be interrupted, errors are inevitable. [0005] As the development of biology and the interdisciplinary processes of biology and other closely related disciplines increase, the variety of kits continues to increase. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions.
问题的解决方案  Problem solution
技术解决方案  Technical solution
[0006] 为了解决现有技术存在的上述问题， 本发明提供了一种多功能的试剂盒。  In order to solve the above problems in the prior art, the present invention provides a multifunctional kit.
[0007] 本发明所采用的技术方案为： 一种多功能的试剂盒， 包括试剂盒本体和盒盖， 所述试剂盒本体上设有发声装置以及控制单元、 小型蓄电池、 显示装置和近距 离无线通讯 NFC标签。 所述发声装置、 控制单元及蓄电池之间电连； 所述显示装 置、 控制单元及蓄电池之间电连； 所述试剂孔外壁设有制冷装置和温控探头， 所述制冷装置、 温控探头与控制单元之间电连； 所述近距离无线通讯 NFC标签由 数据储存模块和数据传输模块组成， 数据存储模块的输出端与数据传输模块电 连， 数据传输模块通过 NFC芯片将信号无线传输至工作人员使用的智能管理终 山 [0007] The technical solution adopted by the present invention is: a multifunctional kit comprising a kit body and a lid, the kit body is provided with a sounding device and a control unit, a small battery, a display device and a close range Wireless communication NFC tag. The sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device and the temperature control probe And the control unit is electrically connected; the short-range wireless communication NFC tag is composed of a data storage module and a data transmission module, the output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the NFC chip to Intelligent management of the use of the staff
[0008] 所述试剂盒本体的内层还设有衬板， 所述衬板上设有移液器 tip头收集孔和试剂 旋转盘， 两者之间还有设有一个箭头指示灯。 所述衬板与所述盒体的幵口留有 一段距离， 使得所述衬板与所述盒体形成一有幵口的容置空间； 所述试剂旋转 盘上设置有多个试剂孔， 并与一动力机构相连。 [0008] The inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.
[0009] 所述试剂盒本体内还设有感应器， 所述感应器与所述移液器 tip头收集孔的底部 对应设置， 并且所述感应器通过感应所述移液器 tip头收集孔的底部控制所述动 力机构带动所述试剂旋转盘的旋转和所述箭头指示灯亮起的颜色。  [0009] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.
[0010] 进一步地， 所述试剂盒本体为双层结构， 所述控制单元、 发声装置和小型蓄电 池设置在试剂盒本体内层与外层之间。  [0010] Further, the kit body has a two-layer structure, and the control unit, the sounding device and the small storage battery are disposed between the inner layer and the outer layer of the reagent cartridge body.
[0011] 进一步地， 所述试剂孔数量为 6个。 [0012] 进- 步地， 所述显示装置为数字显示屏。 [0011] Further, the number of the reagent wells is six. [0012] Further, the display device is a digital display screen.
[0013] 进- 步地， 所述发声装置为蜂鸣器， 数量为 2个。  [0013] Further, the sounding device is a buzzer, and the number is two.
[0014] 进- 步地， 所述控制单元为单片机。  [0014] Further, the control unit is a single chip microcomputer.
[0015] 进- 步地， 所述小型蓄电池为锂离子电池。  [0015] Further, the small battery is a lithium ion battery.
[0016] 进- 步地， 所述感应器与所述移液器 tip头收集孔的底部通过弹簧连接。  [0016] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.
[0017] 进- 步地， 所述试剂旋转盘上设有 8个试剂孔； 所述与之相连的动力机构为步 进电机， 并与感应器相连， 受感应器的控制。  [0017] Further, the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the sensor.
进一步地， 所述箭头指示灯的材质为 LED, 与感应器相连， 并受感应器的控制  Further, the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.
[0019] 进一步地， 工作人员使用的智能终端设于手机上且通过手机界面显示试剂盒的 相关信息。 [0019] Further, the smart terminal used by the staff is set on the mobile phone and displays related information of the kit through the mobile phone interface.
发明的有益效果  Advantageous effects of the invention
有益效果  Beneficial effect
[0020] 本发明通过在试剂盒本体设置发声装置、 控制单元及蓄电池和在试剂孔外壁设 置制冷装置和温控探头， 可为试剂提供所需的预冷环境 （2-8°C) ， 并且可以通 过温控探头检测温度并通过显示装置呈现给实验人员。 一旦超出设定范围， 发 声装置立即报警提醒实验人员， 减少实验失败的机率； 同吋， 通过试剂盘带动 试剂孔旋转， 可以为实验人员起到加样提醒的作用， 避免因加错样导致的实验 失败； 借助智能管理终端与试剂盒可在五厘米的距离内实现非接触式点对点的 数据传输， 解决了传统试剂盒生产管理方法的诸多问题， 提升了管理的效率和 准确性， 为生产企业降低了管理成本。  [0020] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.
对附图的简要说明  Brief description of the drawing
附图说明  DRAWINGS
[0021] 图 1是本发明的整体结构图。 1 is an overall configuration diagram of the present invention.
[0022] 图 2是所述试剂孔的结构图。 2 is a structural diagram of the reagent well.
[0023] 图 3是是如图 1所示的试剂旋转盘、 箭头指示灯和移液器 tip头收集孔的结构示意 图。  3 is a schematic structural view of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.
[0024] 图 4是所述近距离无线通讯 NFC标签的结构示意图。 实施该发明的最佳实施例 4 is a schematic structural diagram of the short-range wireless communication NFC tag. BEST MODE FOR CARRYING OUT THE INVENTION
本发明的最佳实施方式  BEST MODE FOR CARRYING OUT THE INVENTION
[0025] 以下结合附图， 对本发明作进一步说明。  [0025] The present invention will be further described below in conjunction with the accompanying drawings.
[0026] 如图 1所示， 一种多功能的试剂盒， 包括试剂盒本体 10和盒盖 20， 所述试剂盒 本体上设有衬板 30、 发声装置 40以及控制单元 （图中未显示） 、 小型蓄电池 （ 图中未显示） 、 显示装置 80和近距离无线通讯 NFC标签 90。 所述发声装置、 控制 单元及蓄电池之间电连； 所述显示装置、 控制单元及蓄电池之间电连。 [0026] As shown in FIG. 1, a multifunctional kit includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 , a sounding device 40, and a control unit (not shown). ), a small battery (not shown), a display device 80, and a short-range wireless communication NFC tag 90. The sounding device, the control unit and the battery are electrically connected; the display device, the control unit and the battery are electrically connected.
[0027] 如图 2所示， 所述试剂孔的外壁设有制冷装置 381和温控探头 382， 所述制冷装 置、 温控探头与控制单元之间电连。  As shown in FIG. 2, the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.
[0028] 如图 3所示， 所述衬板 30上设有移液器 tip头收集孔 32和试剂旋转盘 36， 两者之 间还设有一箭头指示灯 34， 试剂旋转盘 36上设置有若干试剂孔 38。 感应器 60设 置在盒体 10内， 感应器 60与移液器 tip头收集孔 32的底部对应设置， 并且感应器 6 0通过感应移液器 tip头收集孔 32的底部控制步进电机 70带动试剂旋转盘 36的旋转 和箭头指示灯 34亮起的颜色。 通过上述设置， 感应器 60与移液器 tip头收集孔 32 的底部通过弹簧 50连接。 当加样操作完成后， 废弃的枪头被打入感应移液器 tip 头收集孔 32内， 感应器 60通过感应移液器 tip头收集孔 32的底部， 控制步进电机 7 0带动试剂旋转盘 36旋转和箭头指示灯 34亮起的颜色。 箭头指示灯 34亮起的颜色 与试剂旋转盘 36上的试剂孔一一对应， 红、 橙、 黄、 绿、 青、 蓝、 紫、 白八种 颜色分别对应试剂旋转盘 36上的 1-8号孔。 每次当感应器感应到废弃的 tip头被打 入到移液器 tip头收集孔后， 将控制步进电机 70带动试剂旋转盘 36旋转 45°至其下 一试剂孔， 并且控制箭头指示灯 34亮起所对应试剂孔的颜色。  [0028] As shown in FIG. 3, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is further disposed therebetween, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.
[0029] 如图 4所示， 所述近距离无线通讯 NFC标签 90主要包括数据存储模块 901， 数据 传输模块 902和 NFC芯片 903组成。 数据存储模块的输出端与数据传输模块电连， 数据传输模块通过 NFC芯片将无线将信号传输至用户使用的智能管理终端 （通常 为手机 910) 。  As shown in FIG. 4, the short-range wireless communication NFC tag 90 mainly includes a data storage module 901, a data transmission module 902, and an NFC chip 903. The output of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal (usually the mobile phone 910) used by the user through the NFC chip.
[0030] 本发明通过在试剂盒本体设置发声装置、 控制单元及蓄电池和在试剂孔外壁设 置制冷装置和温控探头， 可为试剂提供所需的预冷环境 （2-8°C) ， 并且可以通 过温控探头检测温度并通过显示装置呈现给实验人员。 一旦超出设定范围， 发 声装置立即报警提醒实验人员， 减少实验失败的机率； 同吋， 通过试剂盘带动 试剂孔旋转， 可以为实验人员起到加样提醒的作用， 避免因加错样导致的实验 失败。 [0030] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once it exceeds the setting range, The sound device immediately alarms the experimenter to reduce the probability of the experiment failure; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, and avoid the experiment failure caused by the wrong sample.
[0031] 本发明不局限于上述最佳实施方式， 任何人在本发明的启示下都可得出其他各 种形式的产品， 但不论在其形状或结构上作任何变化， 凡是具有与本申请相同 或相近似的技术方案， 均落在本发明的保护范围之内。  [0031] The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.
工业实用性  Industrial applicability
[0032] 本发明通过在试剂盒本体设置发声装置、 控制单元及蓄电池和在试剂孔外壁设 置制冷装置和温控探头， 可为试剂提供所需的预冷环境 （2-8°C) ， 并且可以通 过温控探头检测温度并通过显示装置呈现给实验人员。 一旦超出设定范围， 发 声装置立即报警提醒实验人员， 减少实验失败的机率； 同吋， 通过试剂盘带动 试剂孔旋转， 可以为实验人员起到加样提醒的作用， 避免因加错样导致的实验 失败； 借助智能管理终端与试剂盒可在五厘米的距离内实现非接触式点对点的 数据传输， 解决了传统试剂盒生产管理方法的诸多问题， 提升了管理的效率和 准确性， 为生产企业降低了管理成本。  [0032] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by a temperature-controlled probe and presented to the experimenter via a display device. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of failure of the experiment; meanwhile, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the mistake of adding the wrong sample. The experiment failed; the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters, solving many problems of the traditional kit production management method, improving the efficiency and accuracy of management, for the production enterprise Reduced management costs.

Claims

权利要求书 Claim
一种多功能的试剂盒， 其特征在于包括试剂盒本体， 所述试剂盒本体 上设有衬板、 若干试剂孔、 发声装置以及控制单元、 小型蓄电池、 显 示装置和近距离无线通讯 NFC标签。 所述发声装置、 控制单元及蓄电 池之间电连。 A versatile kit comprising a kit body having a liner, a plurality of reagent wells, a sounding device and a control unit, a small battery, a display device and a NFC tag for short-range wireless communication. The sounding device, the control unit and the battery are electrically connected.
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述发声装 置为蜂鸣器。 A multifunctional kit according to claim 1, wherein said sounding means is a buzzer.
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述显示装 置为数字显示屏。 A multifunctional kit according to claim 1, wherein said display means is a digital display.
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述试剂盒 本体为双层结构， 所述控制单元、 发声装置和小型蓄电池设置在试剂 盒本体内层与外层之间。 A multifunctional kit according to claim 1, wherein said kit body has a two-layer structure, and said control unit, sounding device and small battery are disposed between the inner and outer layers of the reagent cartridge body. .
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述衬板上 设有移液器 tip头收集孔和试剂旋转盘， 两者之间还有设有一个箭头指 示灯。 所述衬板与所述盒体的幵口留有一段距离， 使得所述衬板与所 述盒体形成一有幵口的容置空间； 所述试剂旋转盘上设置有多个试剂 孔， 并与一动力机构相连； A multifunctional kit according to claim 1, wherein said liner is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicating lamp is disposed therebetween. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism;
设置在所述试剂孔内的试剂管； a reagent tube disposed in the reagent well;
设置在所述盒体内的感应器， 所述感应器与所述移液器 tip头收集孔的 底部对应设置， 并且所述感应器通过感应所述移液器 tip头收集孔的底 部控制所述动力机构带动所述试剂旋转盘的旋转和所述箭头指示灯亮 起的颜色。 a sensor disposed in the casing, the inductor being disposed corresponding to a bottom of the pipette tip collecting hole, and the inductor controlling the bottom of the collecting hole by sensing the pipe tip The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述箭头指 示灯的材质为 LED, 其特征在于， 所述箭头指示灯与所述感应器连接 ， 并且可以根据需要分别亮起红、 橙、 黄、 绿、 青、 蓝、 紫、 白八种 颜色的光。 A multifunctional kit according to claim 1, wherein the arrow indicator is made of an LED, wherein the arrow indicator is connected to the sensor and can be separately illuminated as needed. Light in eight colors of red, orange, yellow, green, cyan, blue, purple, and white.
根据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述近距离 无线通讯 NFC标签主要是由数据存储模块、 数据传输模块和 NFC芯片 组成。 A multifunctional kit according to claim 1, wherein said short-range wireless communication NFC tag is mainly composed of a data storage module, a data transmission module and an NFC chip. Composition.
[权利要求 8] 据权利要求 1所述的一种多功能的试剂盒， 其特征在于所述数据存储 模块与所述数据传输模块电连， 所述数据传输模块与所述 NFC芯片电 连并通过无线信号将试剂盒的相关数据发送到所述智能管理终端上。  [Claim 8] A multifunctional kit according to claim 1, wherein said data storage module is electrically connected to said data transmission module, said data transmission module being electrically connected to said NFC chip The relevant data of the kit is sent to the intelligent management terminal by wireless signals.
PCT/CN2016/085644 2016-06-14 2016-06-14 Multi-functional reagent kit WO2017214839A1 (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060190185A1 (en) * 1997-08-11 2006-08-24 Ventana Medical Systems, Inc. Memory management method and apparatus for automated biological reaction system
CN203259385U (en) * 2013-03-12 2013-10-30 深圳华大基因研究院 Biological sample distribution error-proofing device and system
CN203465107U (en) * 2013-08-28 2014-03-05 中国水产科学研究院南海水产研究所 Split sample introduction plate mechanism for elemental analyzer
CN204241706U (en) * 2014-12-06 2015-04-01 周振 Infrared induction microwell plate indicator
CN204779612U (en) * 2015-06-09 2015-11-18 盐城卫生职业技术学院 Cell culture board
CN205003156U (en) * 2015-10-20 2016-01-27 苏州荣磐医疗科技有限公司 Utilize quick blood testing arrangement of closely wireless communication transmission

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060190185A1 (en) * 1997-08-11 2006-08-24 Ventana Medical Systems, Inc. Memory management method and apparatus for automated biological reaction system
CN203259385U (en) * 2013-03-12 2013-10-30 深圳华大基因研究院 Biological sample distribution error-proofing device and system
CN203465107U (en) * 2013-08-28 2014-03-05 中国水产科学研究院南海水产研究所 Split sample introduction plate mechanism for elemental analyzer
CN204241706U (en) * 2014-12-06 2015-04-01 周振 Infrared induction microwell plate indicator
CN204779612U (en) * 2015-06-09 2015-11-18 盐城卫生职业技术学院 Cell culture board
CN205003156U (en) * 2015-10-20 2016-01-27 苏州荣磐医疗科技有限公司 Utilize quick blood testing arrangement of closely wireless communication transmission

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