WO2017159461A1 - 飼料組成物、動物プランクトンの製造方法、動物プランクトン、並びに動物プランクトンの成長促進剤及び生残率向上剤 - Google Patents
飼料組成物、動物プランクトンの製造方法、動物プランクトン、並びに動物プランクトンの成長促進剤及び生残率向上剤 Download PDFInfo
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- WO2017159461A1 WO2017159461A1 PCT/JP2017/009034 JP2017009034W WO2017159461A1 WO 2017159461 A1 WO2017159461 A1 WO 2017159461A1 JP 2017009034 W JP2017009034 W JP 2017009034W WO 2017159461 A1 WO2017159461 A1 WO 2017159461A1
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- Prior art keywords
- zooplankton
- pha
- feed composition
- algae
- survival rate
- Prior art date
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
Definitions
- the present invention relates to a feed composition, a method for producing zooplankton, an zooplankton, an zooplankton growth promoter and a survival rate improving agent.
- zooplankton may be fed with algae containing these fatty acids.
- EPA eicosapentaenesan
- DHA docosahexaenesan
- An object of the present invention is to provide a feed composition that promotes the growth of zooplankton, improves the survival rate, and is safe and has little environmental impact.
- the present inventors have intensively studied a feed composition that can promote the growth of zooplankton and improve the survival rate.
- zooplankton can be obtained by feeding polyhydroxyalkanoate (hereinafter sometimes referred to as PHA), which is a biodegradable polyester, alone or mixed with known feed ingredients. It has been found that the survival rate of can be greatly improved.
- PHA polyhydroxyalkanoate
- the present inventors have found that zooplankton can achieve a high growth rate (growth rate) while maintaining a high survival rate by feeding both PHA and algae.
- the first of the present invention relates to a zooplankton feed composition containing PHA.
- the feed composition may include the PHA and algae.
- the feed composition may include at least one kind of algae classified into the genus Chlorella, Nannochloropsis, or Schizochytrium.
- the feed composition may be at least one selected from the group consisting of PHB, PHBH and PHBV.
- the second aspect of the present invention relates to a method for producing zooplankton, wherein zooplankton is bred using the feed composition.
- the third of the present invention relates to zooplankton fed and fed with the feed composition.
- the fourth of the present invention relates to a zooplankton growth promoter comprising PHA as an active ingredient.
- the fifth aspect of the present invention relates to an zooplankton survival rate improver comprising PHA as an active ingredient.
- the growth promoter or the survival rate improver may be at least one selected from the group consisting of PHB, PHBH, and PHBV.
- the present invention relates to a feed composition for zooplankton containing PHA.
- the composition also includes PHA alone.
- PHA is a general term for aliphatic polyesters in which microorganisms accumulate in cells as an energy source, and many substances have been reported as monomer components.
- PHA is originally a natural polymer synthesized by microorganisms by various carbon sources in the natural environment, and is present in soil, the ocean, rivers, and the like.
- Various microorganisms that decompose and utilize PHA have also been discovered, and PHA is a substance that is incorporated into the normal food chain.
- the present invention uses PHA, which is a biodegradable polyester, so that not only there is no problem in safety, but also that zooplankton can be raised by feeding PHA alone as a feed. Is. This is the first time that zooplankton has been found to be able to digest and absorb PHA.
- the PHA used in the zooplankton feed composition of the present disclosure is not particularly limited.
- the PHA is preferably PHA produced by a microorganism.
- PHA is represented by the formula (1): [—CHR—CH 2 —CO—O—] (wherein R is an alkyl group represented by C n H 2n + 1 , and n is 1 or more and 15 or less). It is preferably an aliphatic polyester containing a repeating unit represented by:
- a PHA obtained by polymerizing a component selected from 3-hydroxyalkanoic acids having 4 to 16 carbon atoms and a copolymerized PHA containing a component selected from 3-hydroxyalkanoic acids having 4 to 16 carbon atoms Is preferred.
- polyhydroxybutyrate hexanoate (PHBH) composed of 3-hydroxyalkanoic acid having 4 and 6 carbon atoms, and 4 and 5 carbon atoms
- PHBV polyhydroxybutanoate valerate
- it is at least 1 or more types selected from the group which consists of PHB, PHBH, and PHBV, More preferably, it is PHBH or PHBV.
- the form of PHA may be a culture solution containing PHA-producing cells (bacteria) or a dry cell body, or may be a dry body or suspension of PHA from which cells or cell components have been removed. It is not limited. In addition, it is preferable to use the thing of the magnitude
- the feed composition preferably contains PHA and algae.
- PHA phosphorous sulfate
- algae zooplankton has a high survival rate and can further promote its growth.
- Algae is a general term for organisms that generate oxygen through photosynthesis and inhabit underwater.
- the algae are not particularly limited as long as they can be used as feed, but in particular, floating algae having a size of 1 to 1,000 ⁇ m that inhabit freshwater and seawater excluding large algae are preferable.
- Chlorella Dunaliella, Parachlorella, etc .
- Green algal phylums Nannochloropsis, etc .
- Algae Labyrinthula classes such as Shizochytrium and Aurantichytrium; algae such as Euglena plant gates such as Euglena.
- algae containing nutrient enhancing components such as highly unsaturated fatty acids (DHA, EPA, etc.) are preferable because they can promote the growth of zooplankton and improve the survival rate.
- DHA highly unsaturated fatty acids
- Algae can be used in the form of a culture solution obtained by culturing algae, a dried algal body obtained by drying algae, a product obtained by crushing them, or a culture supernatant of algae.
- a feed composition containing PHA and algae can be obtained, for example, by mixing algae and PHA.
- the PHA and the algae may be a dry body or a suspension, and the form is not particularly limited.
- the feed composition may contain feed components other than PHA and algae.
- feed components are not particularly limited, and known feed components can be used.
- feed ingredients include, for example, highly unsaturated fatty acids (eg, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA), etc.)).
- the feed composition of the present disclosure By giving the zooplankton the feed composition of the present disclosure, it is possible to grow zooplankton early while maintaining a high survival rate. Because live zooplankton can absorb polyunsaturated fatty acids contained in algae such as Chlorella, Nannochloropsis, and Schizochytrium, zooplankton that retains a high survival rate, when fed with such algae, High feed value compared to zooplankton with low survival rate. Thus, according to the feed composition of the present disclosure, it is possible to efficiently and stably obtain zooplankton having a high nutritional value.
- Species of the zooplankton are not limited as long as the effect of improving the survival rate and the growth rate is recognized by feeding the zooplankton with the feed composition of the present disclosure.
- Zooplankton is a general term for micro-organisms that feed on organic matter such as phytoplankton that feeds without photosynthesis and live in water.
- rotifers, artemias, copepods, and daphnia In particular, it refers to those that are generally used as feed for biological feed and seafood in seedling production and aquaculture.
- the rotifer is an organism belonging to the ring-shaped animal gate, and includes the rotifer (Brachionus plicatilis), the S-type rotifer (B. rotundiformis), and the like.
- Artemia is an organism belonging to Arthropoda crustacea, and includes Artemia salina. Copepods and Daphnia are organisms belonging to the arthropoda.
- aquatic organisms such as fish, shellfish, and crustaceans
- fish include small fish such as medaka (Jawa medaka) and sardines, red sea bream, black sea bream, flounder, octopus, yellowtail, cod, salmon, etc. , Crab and the like, but not limited thereto.
- the manufacturing method of the zooplankton of this indication breeds zooplankton using the said feed composition.
- the use of the above feed composition includes, for example, feeding PHA alone as a feed composition to zooplankton, feeding a zooplankton with a feed composition containing algae and PHA, and PHA
- PHA and algae may be a dried product or a suspension, and the form is not particularly limited.
- the mixing ratio or usage ratio of algae and PHA (the usage ratio refers to the ratio of the amount of algae and PHA when feeding at the same time or time difference), the optimal ratio varies depending on the species to be given, and can be arbitrarily set However, for example, PHA is preferably prepared at a ratio of 0.1 to 50% by weight, more preferably 1 to 50% by weight, and still more preferably 5 to 50% by weight with respect to the total weight of algae and PHA preferable.
- the feed composition of the present disclosure is not limited to the time of feeding aquatic organisms, but may be fed through the period from the time after hatching until it grows to the necessary individual, or fed only at a specific time. Also good.
- the specific feeding period varies depending on the target aquatic organism, but is, for example, 2 days or more, preferably 3 days or more, more preferably 5 days or more.
- the upper limit is not particularly limited, and it may be fed for several weeks or months. Specifically, for example, the effect of the present invention can be obtained even for about 3 to 7 days.
- the zooplankton given the feed composition containing the PHA of the present disclosure has a higher survival rate compared to algae, which are commercially available feed / nutrient enrichment for zooplankton. Therefore, the feed composition containing the PHA of the present disclosure can be used as an agent for improving the survival rate of aquatic organisms such as zooplankton.
- a mixture of algae and PHA can be fed to zooplankton to provide PHA alone or algae alone.
- a feed composition containing PHA of the present disclosure preferably a feed composition containing PHA and algae can also be used as a growth promoter for aquatic organisms such as zooplankton.
- the technology for producing a stable zooplankton with high bait efficiency is significant in the cultivation of fish, shellfish, shellfish, etc. that use the zooplankton as food.
- the PHA of the present disclosure has biodegradability, and particularly exhibits excellent biodegradability in water as compared with other biodegradable plastic materials, so it is excellent in that it is safe and has little environmental impact. ing.
- [Zooplankton] Aquatic organisms such as the above zooplankton obtained by feeding the feed composition of the present disclosure have a high growth rate and a high survival rate, and thus are stably and inexpensively supplied as a food for aquaculture such as seafood. can do. Moreover, since it can have a high nutritional value, it is also high in feed value.
- PHBH single feeding test to Artemia salina (Artemia salina) Artemia salina (Artemia salina) after opening for 48 hours or more after hatching treatment was used for the test.
- the test container was a 1 L beaker with a biological density of 50,000 individuals / L.
- the test water was natural filtered seawater (salt concentration: 3.2%), and the seawater temperature was adjusted to 25 ° C. with a plate heater installed under the test container.
- the feed (feed feed) was fed three times in total, 0 hours after the start of the test, 20 hours later, and 40 hours later.
- the amount of salary was 40 mg each time per 50,000 individuals / L.
- the number of repeated lots was 3.
- the breeding water remaining after collecting Artemia salina with plankton net (mesh 125 ⁇ m) prior to feeding the feed at the time of 20 hours and 40 hours after the start of the test is the same as the test water. All water was changed with test water.
- the feed (feed feed) used in the test is PHBH powder (product name: Kaneka Biopolymer Aonilex (registered trademark), manufactured by Kaneka Corporation), and its average particle size (MV) is about 2.95 ⁇ m. A zooplankton was used that was small enough to feed.
- Example 2 Feeding test of PHBH / Schizochytrium mixed feed to Artemia salina Instead of PHBH powder as feed, PHBH (PHBH powder (product name: Kaneka Biopolymer Aonilex (registered trademark), Co., Ltd.) The test was carried out in the same manner as in Example 1 except that Kaneka's)) / Schizochytrium mixed feed was used.
- the PHBH / Schizochytrium mixing ratio was 1: 1 (weight ratio).
- Biochromis manufactured by Taiheiyo Trading Co., Ltd.
- the survival rate of Artemia is shown in Table 1 and FIG. 1, and the total dry weight of Artemia after the test is shown in FIG.
- the growth rate (artemia total dry weight after completion of the test) was similar to that of Example 1 in which PHBH was fed alone, but a decrease in the survival rate was observed during breeding, and 48 After a period of time, the survival rate was as low as about 80%.
- the survival rate decreased after 24 hours from the start of the test, and about 50% of the individuals died after 48 hours.
- the total dry weight of Artemia after completion of the test was significantly lower (low growth rate) than the other test sections. Since the test plot of this comparative example was not fed during the test period, it is considered that the test plot shows a value close to the total dry weight at the start of the test.
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Abstract
Description
[飼料組成物]
本発明は、PHAを含む動物プランクトン用飼料組成物に関する。当該組成物には、PHA単独も含まれる。
本開示の動物プランクトンの製造方法は、上記飼料組成物を用いて動物プランクトンを飼育するものである。上記飼料組成物を用いることとしては、具体的には、例えば飼料組成物としてPHAを単独で動物プランクトンに給餌すること、藻類とPHAを含む飼料組成物を動物プランクトンに給餌すること、及びPHAと藻類を個別に、同時あるいは時間差で動物プランクトンに給餌することなどが挙げられる。PHA及び藻類は乾燥体や懸濁液であってもよく、その形態は特に限定されない。
本開示の飼料組成物を給餌して得られる上記動物プランクトンなどの水生生物は、高育成速度であり高生残率であることから、魚介類等の養殖用の餌として安定的に、安価に供給することができる。また、高い栄養価を有し得るため、飼料価値としても高い。
試験には孵化処理より48時間以上経過した開口後のアルテミア サリナ(Artemia salina)を使用した。試験容器は1Lビーカー、生物密度を50,000個体/Lとした。試験水は天然濾過海水(塩分濃度3.2%)を使用し、試験容器の下に設置したプレートヒーターにて海水温を25℃に調整した。また、飼料(給与飼料)は、試験開始0時間後、20時間経過後、40時間経過後の計3回給与した。給与量は50,000個体/Lあたり各回40mgとした。反復ロット数は3とした。尚、試験開始20時間経過時、40時間経過時における飼料の給与に先立ち、プランクトンネット(目開き125μm)にてアルテミア サリナ(Artemia salina)を回収して残った飼育水を、前記試験水と同じ試験水で全換水した。
給与飼料としてPHBH粉末に代えて、PHBH(PHBH粉末(製品名:カネカ バイオポリマー アオニレックス(登録商標)、(株)カネカ社製))/シゾキトリウム混合飼料を用いた以外は実施例1と同じ方法にて試験を実施した。PHBH/シゾキトリウム混合比は1:1(重量比)とした。シゾキトリウムとしてはバイオクロミス(太平洋貿易社製)を使用した。アルテミアの生残率を表1及び図1に、また試験後のアルテミア総乾燥重量を図2に示す。
給与飼料としてPHBH粉末に代えて、シゾキトリウム(製品名:バイオクロミス)単独を用いた以外は実施例1と同じ方法にて試験を実施した。アルテミアの生残率を表1及び図1に、また試験後のアルテミア総乾燥重量を図2に示す。
給与飼料を全く与えない以外は実施例1と同じ方法にてアルテミアの生育、生残率試験を実施した。アルテミアの生残率を表1及び図1に、また試験後のアルテミア総乾燥重量を図2に示す。
Claims (9)
- PHAを含む、動物プランクトン用飼料組成物。
- 前記PHAと藻類を含む、請求項1に記載の飼料組成物。
- 前記藻類がクロレラ属、ナンノクロロプシス属、又はシゾキトリウム属に分類される藻類のうち少なくとも一種類を含む、請求項2に記載の飼料組成物。
- 前記PHAがPHB、PHBH及びPHBVよりなる群から選択された少なくとも一種以上である、請求項1~3いずれか1項に記載の飼料組成物。
- 請求項1~4いずれか1項に記載の飼料組成物を用いて動物プランクトンを飼育する、動物プランクトンの製造方法。
- 請求項1~4いずれか1項に記載の飼料組成物を給餌して飼育した動物プランクトン。
- PHAを有効成分とする、動物プランクトンの成長促進剤。
- PHAを有効成分とする、動物プランクトンの生残率向上剤。
- 前記PHAがPHB、PHBH及びPHBVよりなる群から選択された少なくとも一種以上である、請求項7又は8に記載の剤。
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CN201780017575.6A CN108779430A (zh) | 2016-03-16 | 2017-03-07 | 饲料组合物、浮游动物的制造方法、浮游动物以及浮游动物的生长促进剂和存活率提高剂 |
US16/085,287 US20190082719A1 (en) | 2016-03-16 | 2017-03-07 | Feed composition, method for manufacturing zooplankton, zooplankton, and zooplankton growth promoter and survival rate enhancer |
JP2018505836A JP6868000B2 (ja) | 2016-03-16 | 2017-03-07 | 飼料組成物、動物プランクトンの製造方法、動物プランクトン、並びに動物プランクトンの成長促進剤及び生残率向上剤 |
EP17766462.0A EP3431579A4 (en) | 2016-03-16 | 2017-03-07 | FOOD COMPOSITION, PROCESS FOR PRODUCING ZOOPLANCTON, ZOOPLANCTON, AND ZOOPLANCTON GROWTH PROMOTER AND SURVIVAL RATE AMPLIFIER |
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WO2019189213A1 (ja) * | 2018-03-27 | 2019-10-03 | 株式会社カネカ | 抗病性飼料、水生生物の生産方法、水生生物、及び抗病性付与方法 |
JP2021061803A (ja) * | 2019-10-16 | 2021-04-22 | 出 角田 | 水産生物用組成物、水産生物の育成方法、及びオリーブ採油粕発酵物の使用 |
WO2022215653A1 (ja) * | 2021-04-06 | 2022-10-13 | 株式会社 フューエンス | ポリヒドロキシアルカン酸(pha)を含む微粒子及びその製造方法 |
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FR3102040B1 (fr) * | 2019-10-21 | 2023-03-31 | Lille Ecole Centrale | Utilisation de nanoparticules d’amidon chargees en molecules actives pour l’aquaculture |
CN111285468A (zh) * | 2020-02-24 | 2020-06-16 | 深圳技师学院(深圳高级技工学校) | 一种浅水型湖泊富营养化及蓝藻水华生物控制方法 |
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- 2017-03-07 US US16/085,287 patent/US20190082719A1/en not_active Abandoned
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WO2022215653A1 (ja) * | 2021-04-06 | 2022-10-13 | 株式会社 フューエンス | ポリヒドロキシアルカン酸(pha)を含む微粒子及びその製造方法 |
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EP3431579A4 (en) | 2019-11-06 |
CN108779430A (zh) | 2018-11-09 |
JPWO2017159461A1 (ja) | 2019-01-24 |
JP6868000B2 (ja) | 2021-05-12 |
EP3431579A1 (en) | 2019-01-23 |
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