WO2017131958A1 - Potentiation of fixed coppers and other pesticides - Google Patents

Abstract

A pesticide composition includes at least one biologically inert carrier; and at least one ferrite or at least one doped component including at least one fixed copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds, magnesium compounds, calcium compounds, and combinations and/or mixtures thereof. In one embodiment, the doped component has a particle size of about 0.5nm to about 30 microns. A method for the control of pests includes the step of applying to the pests or their growth habitat the aforementioned composition. The method also includes the control of fungal and other bacterial and viral diseases in banana plants, by applying the aforementioned composition to their growth habitat in banana plant groves.

Description

POTENTIATION OF FIXED COPPERS AND OTHER PESTICIDES

FIELD OF THE INVENTION

The present invention relates to potentiation of pesticides containing fixed copper in human, agricultural, fungicidal, bactericidal, anti-pathogen and biocidal applications, by increasing the activity of its biocidal effects, so that less polluting copper is used.

BACKGROUND

All Patents, scientific articles and other documents mentioned herein are incorporated by reference as if reproduced in full.

During the 2015 Florida Citrus Show in Fort Pierce, Florida, a 2-day conference, genuine desperation is clearly seen because it is disclosed that most of Florida is infected with Huang-longbing(HLB) bacteria and there are no good treatments for this devastating disease which will all. but destroy the Florida citrus industry. See also. Rusnak, "Ail Hands On Deck to Save Florida Citrus", 2015, http://www.growingproduce.com citrus/insect- disease-update/all-hands-on-deck-to-save-fiorida-citrus/ . Growers were almost crying as they are abandoning their groves, selling their land, pleading with scienti sts to do something, anything, a stop gap measure. At the scientific presentation, the scientists were at a loss to offer anything substantial at this time. Vague promises were made 5 years down the line, etc., that they may have something to counter HLB. Virtually all the growers are hanging on by their fingernails. Moreover, the mirror image of the situation in Florida is being played out in Italy where a similar systemic bacteria is killing centuries old olive trees. The natural course of both diseases is very similar, inescapably progressing to death, and again there are no good treatments. Unless a solution is found within 7 years, there will be little citrus or olive trees in Florida or Italy, respectively. Moreover, with global wanning and climate change there will be many plant pests, insects, bacteria, and fungi extending their range moving into new territory severely impacting the way oflife and the food supply of millions.

An example is the devastating Xylellafastidosa infection of olive trees, in Italy, discussed in "XylellaFastidosa: It's Biology, Diagnosis, Control, And Risks" by J.D. Janse and A. Obradovic. This infection is almost a mirror image of HLB infection of citrus.

Xylella infection is a bacterium, which inhabits the internal vascular compartment of the olive tree, the xylem, and causes like HLB, plants to dry out, die, leaving shriveled stumps, that are incapable of bearing fruit. See also the Daily Mail, in the published article at "Olive Oil Under Threat From Bacteria Which Is Hitting H undreds Of Thousands Of Trees In Italy— And Could Set Prices Soaring" by Hannah Roberts, and "Italian Government Urged To Take Action To Fight Against Olive Tree Epidemic" in Agro News, January 9, 2015, Like HLB, there are few treatments which target the internal vascular system of olive trees,

HLB disease of citrus is a devastating phloem limited incurable bacterial infection which is decimating/killing the citrus industry worldwide. See, "Novel Bactericides and Application Methods to Control Huanglongbing Disease of Citrus" which discusses an overview of "inconsequential effect of nutritional treatments on Huanglongbing control, fruit quality, bacterial titer and disease progress" by T.R. Gottwald, J.H. Graham, et al. See also, "Citrus Disease with No Cure is Ravaging Florida Groves" Lizette Alvarez, New York Times, which provides: "We just need somebody to figure out how we can kill this bacteria in these trees." See also, "Citrus Greening Forces Florida Growers To Trust A Controversial Savior" Huffmgton Post 2013/08/30, which discloses that most commercial growers have adopted foliar nutrition as a stop gap method to fend off the inevitable dying of their citrus trees. See also, "Overview of Citrus Grower Nutritional Spray

Compositions" Tim Spann, which discloses "every fertilizer manufacturer now produces their own program of foliar nutrition for HLB." Further disclosed is the "Maury Boyd cocktail", which is the original nutritional foliar spray for HLB disease support.

Copper (II) hydroxide, also known as cupric hydroxide and having the chemical formula Cu(OH)₂, has a wide variety of commercially important uses, including as a mordant and pigment in dyeing textile and paper fibers, in the preparation of catalysts and other copper compounds, in marine paints, and in fungicides and bactericides. There are tens of millions of pounds of copper hydroxide pesticides, fungicides, bactericides, and biocides used throughout the world yearly, including about three million pounds a year in California alone. The Material Fact Sheet for "Copper Products" in the Organic Resource Guide from the Center for Environmental Fanning Systems,

www. cefs.ncsu.edu/newsevents/...product06-copperproducts.pdf discloses at page 93 that copper is labeled for use on over 100 crop plants to control fungal and bacterial diseases. Page 94 discloses a chart labeled "Copper Studies Showing Fair or Good Efficacy."

As noted in PCT Patent Publication WO 2006028853 Al of Oberholzer, Method for stabilizing copper hydroxide, Publication date March 16, 2006, the patent literature discloses a variety of processes for the commercial manufacture of copper (II) hydroxide. U.S. Patent Numbers 2,924,505, 3,428,731, 3,628,920, and RE 24,324 disclose processes involving phosphate. U.S. Patent Numbers 4,490,337 and 4,808,406 disclose processes involving carbonate; the latter process provides a product comprising considerable copper carbonate, in addition to copper hydroxide. Patent Numbers 1,800,828, 1,867,357,

2,525,242, 2,536,096 and 3,635,668 disclose processes involving ammonia. The processes of U.S. Patent Numbers 2,525,242 and 2,536,096 involve oxidation of copper metal in the presence of ammonia and U.S. Patent Number 4,944,935 discloses a similar process substituting ammonium ion for all or part of the ammonia. The other proce ses start with a soluble copper salt, typically copper (II) sulfate. U.S. Patent Number 4,404,169, European Patent Number EP 80226 BI and PCT Patent Publication WO 02/083566 A2 describe processes starting with copper (II) oxychloride. J. Komorowski-Kulik, Zeszyty, Naukowe Politecniki Sitaskiej, Series: Chemistry 2001, 142, 59-66 discloses a process where an aqueous suspension of copper (II) oxychloride is contacted with aqueous sodium hydroxide in the presence of glycerol as stabilizer. (See PCT Patent Publication WO

2006028853 Al of Oberholzer, Method for stabilizing copper hydroxide, Publication date March 1 , 2006). Oberholzer, in US Patent N umber 7,402,296, claims priority from the aforementioned PCT Patent Publication WO 2006028853 Al of Oberholzer.

Nufarm discloses the history of copper fungicides, the history of copper hydroxide, how copper hydroxide works, how copper works, particle size of copper hydroxide fungicide and more information, including about their products Champ® Dry Prill, Champ® Formula 2 Flowable and Champion® WP (See Nufarm Americas Inc., Nufarm Agriculture Division, "The Copper Champs!" ©2002.)

DuPont discloses a similar product, DuPont Kocide Blue Xtra with similar information. (DuPont (Australia) Ltd., DuPont™ Kocide Blue Xtra with BioActive Copper® ©2006.) DuPont discloses a bewildering array of dozens of plant diseases, treated with DuPont™ Kocide® 3000 Fungi ci de/B acteri ci de, on a multitude of agricultural crops. (E.I. DuPont de Nemours & Company Crop Protection, DuPont™ Kocide® 3000 Fungicide/Bactericide, ©2006-2011.)

Copper pesticides, fungicides, and bactericides are extremely toxic to fish, and aquatic organisms. (Nufarm Americas Inc., Agt Division, Champ® WG Specimen Label). Runoff from the use of copper fungicides, bactericides and algaecides into waterways, ground water and the ground is a very serious contamination problem well known to those in the art. For example, Scientific American, March 18,2013, "Fish Cannot Smell In Polluted Waters" by Brian Bienkowski, discloses: "copper is a poster child for water pollution" said Nathaniel Scholz, an excitology program manager at the National Oceanic and Atmospheric Administration's (NOAA) Northwest Fisheries Science Center, farther noting "copper is intensively used as a pesticide, fungicide.. t's found in cars, in boat paint, so boatyards are often contaminated, and it's often found in industrial discharge and near legacy mining operations. It's a rare pollutant that's both agricultural and urban." Young coho salmon exposed to low levels of copper did not evade predators— cutthroat trout— nearly as well as unexposed salmon, according to a lab study by Scholz and colleagues. The problem is "likely to be widespread in many freshwater aquatic habitats" according to a NOAA report. Copper at low concentrations targets the neurons that help fish, avoid predators, but at higher concentrations, copper impairs their smell for everything.

The Alabama State Water Program, of the Alabama Water Quality Information System—FAQ results, discloses that agricultural, pesticides are considered a potential source of copper pollution for water, and that 10 million pounds of copper was used in agricultural fungicides in the U.S. alone in 1990, "much of the copper is sprayed on plants and. tends to accumulate in the immediate soil environment, making it susceptible to storm water ru off from agricultural operations."

"The Grower", 01/01/2012 by Tom Burfield, discloses that "now, producers are growing increasingly anxious about the effect copper buildup may have on. their groves, and they're increasingly af aid that the day will come when pathogens display copper resistance."

U.S. Patent Number 5,202,353 of Schroth, Iron Enhancement of Copper Based Fungicidal and Bactericidal and Bactericidal Compositions, 1993, discloses that the addition of soluble iron to copper hydroxide fungicide increases activity of the copper hydroxide fungicide bactericide and reverses resistance to copper in vitro. Also, U.S. Patent Number 5,385,934 of Schroth, Methods for Preventing Precipitation of Copper Based Bactericidal Compositions Containing Iron, 1995, discloses the addition of an aggregation inhibiting salt to the copper plus iron compositions to prevent aggregate/or sediment formation upon the addition of Fe+3 to the composition. Both of Schroth's patents taken together require five components - a copper hydroxide component or a fixed copper component, with a dry surfactant, plus a soluble iron component, plus a liquid surfactant, and plus an aggiregation inhibitor, salt. Without being limited, held or bound to any particular theory or mechanism of action Applicant believes that because the copper component and the iron components are separate, the copper hydroxide being insoluble, the iron components being both soluble and insoluble, the aggregation inhibitor, the dry surfactant, and the liquid surfactant, the sizes being vastly different, then it follows mat the release rate and quantities and bioavailability of copper and iron ions on plant surfaces is not identical, or regulated, so that each component may release and disperse their ions at different rates compromising the pesticidal, fungicidal, bactericidal and biocidal effects of the composition. Moreover, it is complicated to have 5 different separate components, with different solubilities; namely a copper component, iron components, two different surfactants, and the aggregation inhibiting component. Schroth's iron component is soluble, so that when sprayed on plant leaves, would tend to disappear in the rain, and thus be of little value. This compelled Schroth to disclose, for example, page 1463, in the sentences before discussion, of Lee and Schroth's paper, "in these experiments, insoluble ferric oxide was used to replace half of the concentrations of ferric chloride for the purpose of increasing persistence." See Lee, Schroth, et al., "Increased Toxicity of Iron- Amended Copper-Containing Bactericides to the Walnut Blight Pathogen

Xanthomonascampestrispv. juglandis" Phytopathology, Ecology ad. Epidemiology, The American Phytopathological Society, 1993, pgs. 1460-1465 (referred to herein as

"Schroth/Lee"). Phytotoxicity was noted on trees treated with Champion® plus both ferric chloride and ferric oxide, although there was no difference in the efficacy between these two treatments. Page 1464 of Schroth/Lee discloses: "the effect of copper-iron mixtures in reducing bl ight of nuts has not been signi ficantly better then copper compounds alone to date" and "whereas copper compounds are very effective in controlling blight of leaves, they have never demonstrated such effectiveness on nuts." Schroth/Lee also concludes on page 1464, "although the addition of ferric chloride to fixed, copper compounds increases the concentration of free copper ions, phytotoxicity has not been observed in the field. However, phytotoxicity occurred when the insoluble ferric oxide was combined with ferric chloride. The reasons for this are unknown but probably have something to do with the long-term release of iron ions that interact with the fixed coppers. This surprising result indicates that much work yet has to be done to find the best formulation that will result in the greatest kill of bacteria over an extended period of time while at the same time not harming tender walnut tissues."

"The effect of iron in increasing the efficacy of copper compounds offers a new advance in the use of the age-old copper compounds and should lead to greatly improved control of bacteria such as X.c.juglandis. However, this will greatly depend on both the ecology of copper-resistant bacteria and the efficacy of new formulations in eradicating established populations."

Schroth's/Lee's paper, and Schroth's patents are clearly not enabling for "new formulations," taken together with the failure of "persistence" with their soluble irons on plant leaves, and unacceptable phytoxicity. When Schroth/Lee tried, to ameliorate the lack of persistence with the addition of insoluble iron to their soluble iron, their paper in the author's own words discloses their invention is unworkable. Their call for "new formulations" says it all, as well as their statement "much work yet has to be done."

Schroth's disclosures have never caught on in commerce because of the complicated nature of their practice and problems with the release of ions, phototoxicity, persistence, lack of activity, rain fastness and unperfected development.

Jim Graham, Megan Dewdney, in "Comparison of Copper Formulations for Control of Canker on Hamlin Oranges" disclose the testing of 14 different dosages of 11 distinct copper formulations. The formulations range from insoluble copper compounds, complexes, chelates to soluble copper chelates. No copper treatment was very effective on fruit incidents at harvest, A preferred size for systemic uptake according to Graham is 5 to 10 nm. See Graham, Jim, Novel B ctericides and Application Methods to Control Disease of Citrus, IV international Symposium of Plant-Pathogenic Bacteria, Guadalajara, Jalisco, Mexico, September 23, 2014, University of Florida, UF-IFAS, especially third page from end, entitled Alternative bactericide must be non-phytotoxic and systemic, i.e., capable of loading into the phloem via foliar application. No insoluble coppers tested by Graham had a particle size of 5-10 nanometers, so that none of the compounds of Graham's testing are capable of systemic administration to the plant throug the stomata.

Zinc-doped CuO nanocomposites of a specific size are known for use in specific fields. Eyal Malka et al., (small 2013, DOl:10.i002/s:mll.201301081, www.small- joumal.com) discloses, "eradication of multi-drug resistant bacteria by a novel zinc-doped CuO n ano composite." Michal Eshed, et al., (Advanced Functional Materials 2014, pp.1382-1390, www.afm-journal.de) discloses, "a Zn-doped CuO nanooomposite shows enhanced anti-bio film and antibacterial activities against Streptococcus mutans compared to nanosized CuO." They conclude, "the results of the present study further highlight the potential of these novel Zn:CuO np's as inhibitors of biofilm within the context of the oral niche." Moreover, Richardson provides, "commercially produced cupric oxide (copper(2) oxide) is ineffective as a fungicide or bactericide due to small surface areas; that is, dissolution times are very long compared to other basic copper (2) compounds," see H. Wayne Richardson in the "Handbook. Of Copper Compounds And Applications" pg. 109. This CuO used by Malka and Eshed is the same copper referred by Richardson above. While 30nm sized Zn:CuO nanocomponents are known, Applicant submits that taken together, biofilm in the oral niche and coatings of linens and antimicrobial bandages _s they do not relate in any way to agricultural pesticide, and systemic plant protectorants.

Moreover the size of 30 nm previously disclosed does not conform to Graham's teaching of 5 -10 nm for systemic administration to citrus plants.

Ploss, et al, United States Patent 7,1.05,1 6 B2, discloses that doping 5 wt % zinc metal, into a copper salt composition intended for agricultural applications provided enhanced surface adhesion, as in the case of plants on leaf and fruit surfaces, thereby increasing the duration of the plant-protecting effects and also eliminating the expense and environmental emissions associated with the re-application of prior art compositions that would be required to provide the same level of protection. Floss's use of metallic zinc to provide enhanced surface adhesion does not teach any ad vantages with regard to pesticidal characteristics per se. For example, zinc is a metal, an element and is insoluble in water, with a small surface area. Ploss provides no teaching or suggestion that its metallic zinc is pesticidal as "enhanced, surface adhesion" is clearly taught. This is known to those of knowledge of the art as an "adjuvant." Exemplary examples of the overwhelming plethora of agricultural adjuvants are: Nufarm, "Adjuvants Product Guide" pg 16, and Momentive, Momcntivc Adjuvants, Silwct and Agrosprcad adjuvants. Nufarm's Spraymate bond is a high quality sticker, deposition and retention agent for use with contact or protectant type fungicides or with contact and ingested insecticides. Spraymate bond increases adherence of spray droplets by sticking them firmly to target surfaces. This protects pesticides against wash-off by rain or sprinkler irrigation. Spraymate bond also protects chemicals by slowing the rate of degradation immediately after application. Its key benefits are that it improves spray droplet deposition, retains and protects droplets on targets, improves the performance and life of protectant fungicides and contact insecticides. Furthermore, there is a world of difference between pure zinc metal, an element, and insoluble pesticidal zinc compounds. SUMMARY OF THE INVENTION

This invention is directed to a pesticide, fungicidal, bactericidal, anti-pathogen or biocidal composition including a) at least one biologically inert carrier and; b) at least one doped component comprising at least one fixed copper compound, such as copper hydroxide, doped with at least one compound selected from the group consisting of iron compounds, zinc compounds, magnesium compounds, calcium compounds, and combinations tliereof. In a preferred embodiment, the doped component only includes iron compounds and/or zinc compounds as the only doping compounds. Preferably, the doped component has a particle size of about 0.5 run to 30 microns. The iron compound and/or zinc compound are doped into the fixed copper compound, such as copper hydroxide, singly or together in combination. In one embodiment a fixed copper compound is doped with an insoluble iron compound, such as, for example, where the fixed copper compound, is copper hydroxide, and wherein the fixed copper compound is partially substituted by an insoluble iron compound. Optionally, a surfactant may be included in the composition to provide for a more efficacious formulation.

One advantage of Applicant's invention is that the composition includes doped components that contain both the fixed copper compounds and the iron, zinc, magnesium, and/or calcium compounds combined on the doped component. The fixed copper compound, such as copper hydroxide, doped with the iron compound and/or zinc compound is used in order to provide a more cost effective composition with increased activity as compared to copper hydroxide alone. Therefore, less copper is required, with its resultant reduced runoff, and less pollution derived from copper. Moreover, iron is a plant micronutrient and the present composition would supply iron to plants as against copper hydroxide alone. It has been found that: heavy use of copper compounds produces iron chlorosis. In addition, copper hydroxide is a pesticide, rungicide/bactericide that is so well established so that the present invention could be administered very similarly to the current methods of copper hydroxide administration with a straight substitution, more or less, except that the amount of elemental copper derived from the present invention will be reduced, as against stand-alone copper hydroxide, to achieve the same or greater control or pathogen reduction. In one embodiment of the present invention, Appli cant submits that the present invention may be substi tuted for the copper hydroxide in PCT Patent

Publication WO 2006028853 to Oberholzer. The composition of the present invention also reverses resistance to copper so that copper resistant pathogens become sensitive to copper. While the Material Data Sheet for Copper Products, page 94, discloses a list of crops with poor results with copper, it is believed that Applicant's present invention increases the activity of copper with respect thereto. In one embodiment, Applicant's present invention may be provided as a pesticide, fungicide, bactericide, anti-pathogen and biocidal nutrient spray with optional additional micronutrients and/or optionally macronutrients, so tiiat there would be a combination copper based pesticide with micronutrients all in one formulation. In another embodiment, Applicant's present invention includes an insoluble copper pesticide, fungicide, bactericide, and anti-pathogen and biocidal capable of systemic uptake by foliar, root and/or trunk injection, and a plant protectorant, dual mode of action, all in one compound.

The prior art teach es various complexes of copper compounds, mixtures which are two or more substances that are not chemically combined, and chelates of copper compounds. Compounds of the present invention are overwhelmingly, compellingly, and persuasively, distinguishable, as doped zinc compounds and doped iron compounds are internalized in the copper hydroxide or other fixed coppers. Complexes and chelates are joined so that they are externalized to the copper hydroxide or other fixed coppers.

Moreover, being externalized to the copper hydroxide or other fixed copper produces larger particulates undesirable for systemic administration.

Applicant's doped components can also be sized at about 5nni-10nm, and other larger particulates, in one embodiment, to provide both plant protectorant and systemic uptake to be carried internally through the plant phloem and xylem to kill systemic bacterial infections such as HLB, and Xylcllafastidosa. and other systemic pathogenic fungi, or internal pathogens. Applicant's doped composition of fixed coppers doped with zinc, iron, or both, are also useful in treating banana plants afflicted with the Sigatoka complex of banana fungal diseases, including Black Sigatoka disease, Yellow Sigatoka disease, and eumusae leaf spot disease, as well as also Panama disease. In general, the composition is useful in treating banana fungal diseases such as, for example, but not limited to, Black Sigatoka disease, Yellow Sigatoka disease, leaf spot Sigatoka disease, Panama disease, Fusarium wilt, Antliracnose, Armillaria corn rot, Black cross, Black root rot, Brown blotch, Brown spot, Ceratocystis fruit rot, Cigar-end, Cladosporium speckle, Conn, dry rot, Crown rot. Cylindrocladium root rot, Damping-off disease, Deightoniella fruit speckle, Diamond spot, Dwarf Cavendish tip rot, Eyespot, Fruit freckle, Fruit rot, Fungal fruit rot, Leaf rust, Leaf speckle, Main Stark rot, Malayan leaf spot, Marasmiellus rot, Peduncle rot, Pestalotiopsis leaf spot, Phaeoseptoria leaf spot, Pitting disease,

Pseudostem heart rot, Root and rhizome rot, Sclerotinia fruit rot, Septoria leaf spot, Sheath rot, Sooty mold, Speckle disease, Squirter black end. disease, Stem-end rot, Trachysphaera finger rot, Tropical speckle and Verticillium tip rot. The composition is also useful for treating bacterial diseases of banana plants, , such as, for example, but not limited to, Xanthomonas wilt, Banana bacterial wilt, Enset wilt, Blood disease, Bugtok disease, gumming finger tip rot, Moko disease, Rhizome rot, and Japanese vascular wilt disease. The composition is also useful for treating viral diseases in banana plants, such as, for example, but not limited to, Bract mosaic disease, Banana bunchy top disease, Abaca mosaic disease, Banana streali virus disease, Badnavirus, viral caused chlorosis, Cigar leaf necrosis, cucumber mosaic virus, Sugarcane bacilliform virus, and banana virus X disease.

The Applicant's invention clearly will not displace and replace the entire world use of pesticides. However, applicant's invention will clearly replace and displace many of the highly toxic pesticides currently being used against pests which are resistant to existing copper pesti cides .

Moreover the Applicant's invention will decrease the breadth, depth and range of pesticides currently resistant to copper pesticides, restoring sensitivity and high activity. Moreover a Google search discloses over 52 million hits for the well-known established harmful effects of pesticides.

M reover, Wikipedia discloses, "Environmental Impact of Pesticides" with one page and a half of horrific pesticide environmental effects of classes of pesticides. In contrast, the Applicant's present invention reduces the need for the quantity of copper currently being used as a pesticide and displaces and replace many current highly toxic poisonous pesticides currently being used. Copper is the lesser of the two evils as against synthetic agricultural chemical pesticides with their well-known toxicities and horrific environmental effects.

BRIEF DESCRIPTION OF THE DRAWINGS:

[ The preferred embodiment of the present invention can best be understood in connection with the accompanying drawing. It is noted tlmt the invention is not limited to the precise embodiments shown in drawings, in which:

FIG. 1 is a copy of an X-Ray Diffraction test showing copper hydroxide doped with. 1 at% insoluble iron as a dopant in accordance with one embodiment of the present invention. There are no additional peaks proving that the iron is incorporated within the copper hydroxide.

FIG. 2 is a transmission electron microscopy (TEM) image of Applicant's iron doped copper hydroxide at 15 at% iron in accordance with one embodiment of the present invention. The scale on the lower left of FIG. 2 shows a comparable length of 50 nm. The size of the iron doped 15 at% Fe copper hydroxide particulates is about 3.5 nm to 9 nm, tested in quadruplicate and by transmission electron microscopy (TEM).

FIG.3 is an elemental analysis chart demonstrating the presence of both iron and copper in the iron doped copper hydroxide compound in accordance with one embodiment of the present invention of FIG, 1 and FIG. 2 above.

FIG. 4 is the X-ray diffraction of 15 at% iron doped copper hydroxide with an additional 10 at% iron that the copper hydroxide is unable to incorporate, so that the final composition is 15 at% iron doped copper hydroxide + 10 at% unincorporated/free iron hydroxide and/or iron oxide.

DETAILED DESCRIPTION OF THE INVENTION

The invention is a pesticide, fungicidal, bactericidal, anti-pathogen or biocidal composition comprising a) at least one biologically inert carrier; and b) at least one doped component including at least one fixed copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds, magnesium compounds, calcium compounds and combinations thereof. In a preferred embodiment, the doped component only includes iron compounds and/or zinc compounds as the only doping compound. For example, copper hydroxide may be doped with iron hydroxide, iron oxyhydroxide or other iron compound as one doped component. In one embodiment, the fixed copper compound is doped with an insoluble iron compound, wherein the fixed copper compound is partially substituted by the insoluble iron compound. The invention also comprises a fixed copper compound, such as copper hydroxide, doped, with a zinc compound. Furthermore, the fixed copper compound, such as copper hydroxide, may be doped with both an iron compound and a zinc compound. Applicant's invention, includes at least one doped component containing both, the fixed copper compound, doped with an iron compound and/or a zinc compound that can be used similarly to how copper hydroxide alone is used. In one embodiment of the present invention, an iron compound is doped on copper hydroxide, a zinc compound is doped on copper hydroxide, a zinc compound and an iron compound are doped on copper hydroxide, singly and optionally combined together, and or added to other pesticide agents. The doped component is prepared according to methods described herein and readily understood to those of skill in the art. The composition can be used in many of the applications that copper hydroxide is currently utilized, and many new pesticidal indications which are currently resistant to copper. See, for example, WO2006028853. In a preferred embodiment, copper hydroxide is doped with iron hydroxide to provide 15at% iron to the doped component, which results in a black doped component. In another preferred embodiment, the atomic percentage (at%) of iron in the doped component is less than 15 at %, more preferably less than 10 at%. Because of the increased biocidal activity, the preferred copper hydroxide-iron hydroxide doped component prepared by the present method is especially useful as an active ingredient in pesticides, fungicides, bactericides and biocides. This increased biocidal acti vity reduces the number of pathogens currently resistant to copper pesticides, increasing the range and breath, of activity of copper against other bacteria, fungi, viruses, mycoplasma, and other pathogenic organisms. Unlike mixtures, doped compositions cannot be separated by physical means. The dopant is incorporated within the structure of the compounds of interest, and becomes part of it. For example, Prabhakaran and Boothroyd, "Single crystal growth of Zn-doped CuO by the floating-zone method," Journal of Crystal Growth 250 (2003) pp. 77-82, describes the preparation of Zn-doped CuO, in order to obtain "sizable single crystals of high purity." On page 79, the authors note that "Zn is incorporated in the lattice and as reported elsewhere," where the lattice is the arrangement of the ions, atoms or molecules of the substance. Simply stated, a dopant substitutes in, and take the place of, elements that were in the lattice of the base material. Prabhakaran, et al., also notes that sometimes there is residual left out ZnO impurities when doping CuO, because of having exceeded the solubility limit of the dopant ZuO into the lattice of the CuO.

Likewise, in Haldolaarachchige, et al, "Effect of Chemical Doping on the Thermoelectric Properties of Fe Gaz," Journal of Applied Physics, March 2011 , describes that doping increases carrier density and results in a new metallic state. Haldolaarachchige, et al., also disclose the preparation of the chemically doped FeGaz doped with either Co or Ge. For example, Haldolaarachchige, et al., states in "IV. Conclusions" as follows: "Even small amounts of chemical doping have a significant effect on the physical properties and electronic structure of the pure compound."

Preferably, the composition is administered to plants by a method selected from the group consisting of dusting, sprinkling, spraying, brushing, dipping, smearing, impregnating, injection of the composition into plant vasculature, and application to a root system.

DEFINITIONS

In the context of this application, several terms are utilized as follows:

The definition of "pesticide" used in this application refers to the United States

EPA definition page 1 ofwww.epa.gov/agt culture/tpes.html which is "A pesticide is any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest. Pests can be insects and insect-like organisms, mice and other vertebrate animals, unwanted plants (weeds), or fungi, bacteria and viruses that cause plant diseases. Though often misunderstood to refer only to insecticides, the term pesticide also applies to herbicides, fungicides, and various other substances used to control pests."

The definition of a "mixture": a mixture contains 2 or more substances that are not chemically combined. Mixtures are unlike chemical compounds, because: the substances in a mixture can be separated using physical methods such as fdtration and distillation. Mixtures have variable compositions while compounds have a fixed definite formula.

When mixed, individual substances keep their properties in a mixture, while if they form a compound their properties can change. See

http://www.chemicool.com/defmition/mixture.html). An example of a mixture is

NORDQX 30/30 wg, manufactured by NORDOX AS c/o Monterey AgResources, EPA Reg. No. 48142-7), which is a cuprous oxide and zinc oxide mixture.

The definition of "elements" and "compounds": "Elements and compounds are pure chemical substances found in nature. The difference between an element and a compound is that an el ement is a substance made of the same type of atoms, whereas a compound is made of different elements in definite proportions. Examples of elements include iron, copper, zinc, hydrogen and oxygen. Examples of compounds include water (H₂0) and salt (sodium chloride-NaCl)." See,

http://www.diffen.com/difference/Compound_vs__Eleraent.

The definition of "complex": "A complex is a molecular entity formed by loose association involving two or more component molecular entities (ionic or uncharged), or the corresponding chemical species. The bonding between the components is normally weaker than in a covalent bond. In inorganic chemistry the term "coordination entity" is recommended instead of "complex" (IUPAC inorganic NOMENCLATURE (1990)." See, http://¾ww.ehemicool.conVdefinition/complex.hrml. The definition of "chelate"; "In reality, a chelate is any metal that is attached to an anion (negatively charged group) with more than one attachment site. Chelation means that the anion has two or more separate sites to which the metal is bonded.'¹ See,

www.j o stchemical . com.

The definition of a "dopant": a dopant is "an impurity added usually in minute amounts to a pure substance to alter its properties." See, www.merriam- webster.com/dictionary/dopant. According to www.freedictionaiy.com, under "medical" the term "to dope" is used in materials sciences as a verb which means "To add or incorporate a substance or other additive to a chemical compound of interest, with the intent of improving or altering performance parameters. "

The definition of "fixed copper": a copper compound which is insoluble or highly insoluble in water. Fixed Coppers are further disclosed by H. Wayne Richardson in the "Handbook Of Copper Compounds And Applications", page 97 tliird paragraph, "suitable copper compounds include fixed coppers [Cu+(OH)2], Bordeaux, as well as other well known fixed copper compositions including those disclosed in CRV Handbook of Pest Management in Agriculture, Vol. 3, David Pimentel (editor), CRC Press, Boca Raton, FL (1981 ), which is incorporated herein by reference" and Sabin Ί96 patent such as copper oxide/cuprous oxide, copper oxychloride, tribasic copper sulfate (CuS0₄ 3Cu(OH)2, copper diammonia diacetate complex and/or any other well-known fixed or insoluble copper compositions currently being used, or has been prior used as a pesticide, fungicide, bactericide, and algaecide. Exemplary examples are copper hydroxide, copper/cuprous oxide, copper carbonate, copper oxychloride, basic carbonate, copper carbonate, basic copper sulfates including particularly tribasic copper sulfate, copper oxychloricles, and mixtures thereof, ammonia copper carbonate, basic copper chloride, and others, and mixtures thereof.

The term "iron doped copper hydroxide" refers to a copper hydroxide compound that is doped with an iron compound. It is understood in alternate embodiments that the copper hydroxide compound may be substituted with another fixed copper compound as provided herein.

The term "zinc doped copper hydroxide" refers to a copper hydroxide compound that is doped with a zinc compound. It is understood in alternate embodiments that the copper hydroxide compound may be substituted with another fixed copper compound as provided herein. The term "zinc and iron doped copper hydroxide" refers to a copper hydroxide compound that is doped with both an iron compound and a zinc compound. It is understood in alternate embodiments that the copper hydroxide compound may be substituted with another fixed copper compound as provided herein.

Examples of iron compounds include any insoluble or substantially insoluble iron compound. Further examples include, but are not limited to iron compounds selected from the group consisting of iron hydroxide, iron oxyhydroxide, iron oxide, iron glucose, ferric citrate, Ferritin, ferrous firmarate, and ferrous sulfate. Most preferably the iron compound is iron hydroxide and/or iron oxyhydroxide.

Examples of zinc compounds include any insoluble or substantially insoluble zinc compound. Further examples include, but are not limited to zinc compounds selected from the group consisting of zinc hydroxide and zinc oxide.

In a preferred embodiment, the term "insoluble or substantially insoluble" is used herein. However, based on industry standards, Applicant submits that there is not a hard and fast quantitative definition for such terms as used by those of skill in the art.

Accordingly, terms such as "sparingly soluble", "insoluble", "highly insoluble", "slightly soluble", ("negligible") etc. have been used for decades by those of skill in the art. See, for Example US 5,385,934, at column 1, lines 28-38, wherein Professor Emeritus Milton N. Schroth provides that "These copper based compositions are typically aqueous" fixed" copper based compositions because the copper compounds used in these compositions typically have a solubility of free Cu+2 from about 1 to 30 ppm in the aqueous solution with the remainder (and the vast majority) of the copper either being insoluble or in chelated form (i.e., "fixed"). The 1 to 30 ppm of Cu +2 in such aqueous compositions is typically referred to as "free copper" to distinguish it from either the chelated Cu+2 or the insoluble Cu+2 in these fixed copper compositions." Similarly, Professor Santra discloses in US 8221791 Bl "An important consideration is whether to use "soluble" or "insoluble" coppcr(Cu) for long term fungicidal or bactericidal protection. The "soluble" Cu refers to Cu based, salts (such as Cu. sulphate) that hydrolyze completely in water, producing ionic Cu. The "insoluble (sparingly soluble) Cu compounds act as a reservoir from, which Cu ion is released to the plant surface on which it is deposited upon application."

Additionally, Professor Santra provides "Currently used Cu compounds possess unique set of physical and chemical properties. They differ in their total amount of metallic Cu content and aqueous solubility. It is well understood that the antibacterial activity will depend upon the availability of soluble (free and reactive) Cu ions in the formulation. Among the existing Cu compounds, tribasic Cu sulphates and cuprous oxide are least soluble, whereas Cu hydroxides are more soluble than Cu oxychloride." And Professor Santra further provides "Several Cu compounds are registered in the United States for management of over 100 diseases on almost 50 food crops. The Cu compounds exhibit varying degrees of effectiveness for any target organism on any given host. The most common forms of Cu that satisfy these conditions to varying degrees are the normal hydrolysis products of Cu(l) and Cu(2) salts (also known as "insoluble Cu" of "fixed Cu" compounds: Cu(l) oxide, (Cu₂0), Cu(2) oxychloride(CuCl₂.3Cu(OH)₂), tribasic Cu(2) sulphate(CuS043Cu(OH)2, and Cu liydroxide(Cu(OH)₂." In Agrochemical and Pesticide Safety Handbook, Michael F Waxman discloses "Since copper is toxic to plants, it must be used at low levels or in the insoluble form. For this reason, the relatively insoluble or "fixed" copper salts are used. These compounds release copper ions at very low rates that are adequate for fungicidal activity but not at concentrations that would harm or kill host plant." Richardson discloses "Alternate products were developed primarily in the twenties and thirties and relied on low soluble or fixed coppers which could be applied as dusts or suspensions" and "A copper compound must be chosen that is relatively resistant to weathering and supplies enough copper to be toxic to the fungal spores and bacterial cells without adversely affecting the host. The most common forms of copper that satisfy tliese conditions to varying degrees are the normal hydrolysis products of copper(l ) and copper(2) salts: copper (1) oxidc(Cu2),cuprous oxide), copper(2)

oxychloridc(CuCl₂.3Cu(01i)2, tribasic copper(2)sulphate(CuS04.3Cu(OH)₂, and copper hydroxides(Cu(OH)₂). These "fixed coppers" offer advantages of application and reduced phytotoxicity over the classic Bordeaux mixture. "These are the terms Richardson uses: Pg 85, Copper(2) Phosphate Trihydratc, "insoluble in cold water", Pg 55, Copper(l) Oxide, "virtually insoluble in water" Copper(2) oxide, Pp. 57,58, "essentially insoluble in water", Pg 61, Copper(2)Hydroxide, "virtually insoluble in water", Pg 63, Copper(2) carbonate Hydroxide, "virtually insoluble in water", Pg 69, Copper(2) Oxychloride, "essentially insoluble in water", Pg 79, Basic Copper(2) sulphate, "insoluble in water", Pg 83, Copper(2) Gluconate, "soluble in water." Richardson discloses a multitude of Copper compounds and descriptive nomenclature describing their solubility as above

demonstrated.

Those of the skill in the art can readily and easily test to determine the lower doses required of the applicant's invention to achieve disease control, and the doses to achieve disease control on copper resistant pathogens by standard routine testing.

The iron doping of the copper hydroxide nanoparticles is from a trace

contamination of iron, less than .01 at%, to about 40 at% iron with about 5-1.5 at% elemental iron preferred, with about 15 at% elemental iron the most preferred quantity. Other fixed coppers may incorporate more than 40 at% iron and/or zinc. While a variety of processes may be used to manufacture the doped component of the present in vention, Applicant has used a wet chemical process as further indicated in detail below. It is understood to those of skill in the art that the process disclosed herein is scalable for commercial production. The product is dried/evaporated by methods well known to those skilled in the chemical art, and maybe overcoated, if desired, with a stabilizer by methods well known to those in the chemical art. For example, See US Patent 4,404,1.69 to Ploss, et al. entitled "Process For Producing Cupric Hydroxide." The preferred size of an iron doped copper hydroxide nanoparticle is sub-micron, from about 0.5nm to 30 microns. A more preferred size is from about 3.5nm to 15 microns, an additional preferred size is from about 3.5nm to 200nm. A most preferred size is from about 3.5nm to lO m, especially for both leaf pro tectorant and systemic activity all in one. Different sizes may be mixed together in the practice of the invention.

Moreover, fixed copper compounds, such as copper hydroxide, are generally ^■ insoluble or highly insoluble in water. While not being limited, held or bound to any particular theory or mechanism of action, it is generally thought that "The free copper penetrates into the bacterial and/or fungal micro-organism in order to exert its toxic effect." (See U.S. Patent Number 5,202, 353 of Schroth, Iron Enhancement of Copper Based Fungicidal and Bactericidal and Bactericidal Compositions, 1993). Applicant believes, in addition, that the generation of reactive oxygen species (ROS) by fixed copper compounds and the additional increase in ROS generation with the addition of an iron compound is pesticidal. Moreover, while not being limited held or bound of any particular theory or mechanism of action, it is thought that exudates on the surfaces of the plant leaves, taken together with the rainfall and the acidic rain, produce an. acidic environment which dissolves the fixed copper compounds and releases free copper, which is very active against pathogens.

Thus without being limited, held or bound to these plant diseases disclosed, there are hundreds and hundreds of plant diseases amenable to control by copper pesticides and that the applicant's invention will surely potentiate the biocidal effects of copper pesticides against these pests. The composition of the present invention may be used with any known biologically inert carrier, including, but not limited to, a liquid diluent, e.g., water, a solid diluen t and/or a surfactant. The composition of the present invention is designed to be compatible with the physical properties of copper hydroxide and any other active ingredients, metliod of application and environmental factors which may include soil type, moisture and temperature, organic matter, soil structure, current nutrient levels and more, well known to those in the chemical and agricultural art.

Moreover, without being limited held or bound to any particular theory or mechanism of action, the more iron added to the copper hydroxide or other fixed copper, the more biocidal/robust pesticide the invention will be toward pests.

Moreover, in. one embodiment of the present invention herein, Applicant's iron doped copper hydroxide, or zinc doped copper hydroxide, or zinc and iron doped copper hydroxide, in an optional embodiment, may be stabilized or overcoated. United States Patent 4,404,169 PIoss, et al. discloses methods of stabilizing compounds of the applicant's invention. Overcoating, can modulate release of the active ingredient.

In an alternate embodiment, insoluble iron compounds, such as iron hydroxide or iron oxyhydroxide, may be added to copper hydroxide so that there are two separate components. In a further alternate embodiment, iron hydroxide or other insoluble iron compounds, may be added to the iron doped copper hydroxide, so there are also two separate components. In each case, evciything goes together in the spray tank for agricultural uses.

Moreover, since iron and copper are recognized micronutrients, recognized by the American Society of Agronomy and the Soil Science Society of America, then it follows that the remaining micronutrients boron, chloride, manganese, molybdenum and zinc, can easily be added to the iron or zinc or iron and zinc doped copper hydroxide, which uses iron and or zinc as a dopant to produce a doped copper hydroxide iron

fungicide bactericide/nutrient with micronutrients for foliar, trunk, branch and/or root application and/or injection in the trunk or branches of the plant. Moreover, secondary nutrients such as calcium, magnesium and sulfur may also be added, singly or together in combination,

Applicant's invention is the only known foliar nutritional bactericidal formulation that enters the phloem, and kills the HLB disease causing CandidatusLiberibacter (CLas) bacteria.

Since zinc has established anti-microbial activity against bacteria, fungi and other pathogens, Applicant proposes to potentiate, increase activity of the cl aimed iron doped, copper hydroxide by further doping the copper hydroxide with a zinc compound so that the end product will be an iron doped, zinc doped copper hydroxide or other fixed copper. It is understood that the doping with zinc is provided by a zinc compound and not by the use of pure metallic zinc, an element.

Preferred embodiments of this invention include both a zinc compound doped copper hydroxide and an iron compound doped copper hydroxide. Zinc sulfate is an exemplary example of a zinc salt thought to be useful in the manufacture of compounds of the invention. The sulfur in zinc sulfate is also a plant nutrient. Applicant also claims copper hydroxide which is both doped with an iron compound and a zinc compound together. In the preparation of the iron doped copper hydroxide, excess iron more than that the copper hydroxide will incorporate, is left in the preparation, so that the upshot is iron doped copper hydroxide, with additional iron hydroxide, in the preparation thereof. It may very well be an iron oxyhydroxide or other iron compound incorporated in the doping process.

Iron doped copper hydroxide, with and without additional unincorporated iron hydroxide in the preparation, may be combined with zinc doped, copper hydroxide with and without additional unincorporated zinc hydroxide or other zinc compounds, and both zinc and iron doped copper hydroxide with both additional unincorporated zinc and iron may also all be used in combinations or mixtures thereof. Other useful compounds are ferrites, which may be an additional alternate compound to be used stand alone, or may be combined as the iron and/or zinc doped copper hydroxide is also used. A ferrite is a type of ceramic compound composed of iron oxide

combined with one or additional metallic elements in chemical combination. See Encyclopedia Britannica. See Wikipedia. Exemplary examples are copper zinc iron oxide (CuZnFe₂.04). See Sigma order confirmation, order date 04/06/2015, order number 3013972345. This order also contains a copper iron oxide ferrite (CuFe₂C>4). See Sigma Technical Services answer of Jeanne Harrell, "X-ray Diffraction Results", dated 04/14/2015. Other ferrites include zinc iron oxide nanopowder (ZnFe₂0₄). See American Elements 15041 RP03, dated 04/14/2015. Another ferrite is a copper ferrite (CuFe^O^. See American Elements data sheet for copper ferrite, dated 04/16/2015. There may be manganese in the composition of useful ferrites. While not being limited, held or bound, the aforesaid ferrites are exemplar examples thought to be useful in the practice of the invention, and may be combined in any combination with themselves or other compounds previously disclosed in the practice of the invention. Preferably, tine size of the ferrites are from 0.5 nm up to 30 microns.

These combinations or mixtures thereof are easily tested against bacteria, fungi, viruses, mycoplasma and other pathogens, by rapid, well established, routine testing well known to those skilled in the art, such as laboratory high throughput screening of vast numbers of compounds in vitro, and/or greenhouse plantings.

The compositions of the Applicant's invention are especially directed and exquisitely suited as a treatment for Huanglongbing (HLB) disease of citrus, and citrus canker, and other citrus diseases, such as Greasy Spot, Melanose, and Alternaria Brown Spot, where copper is currently utilized. Applicant's doped composition of fixed coppers doped with zinc, iron, or both, are also useful in treating banana plants afflicted with Black Sigatoka disease, Yellow Sigatoka disease and Panama disease.

Moreover, the small size of Applicant's invention will improve foliage coverage of leaves, which is well known in the art:, that small particulates provide more uniform and superior coverage to leaves of plants. A critical and. distinguishing feature of the

Applicant's invention is that in preferred embodiments it can be an ultra-small insoluble copper and iron compound. Moreover, another critical aspect of Applicant's invention is the size of Applicant's iron doped copper hydroxide such that it is enabled for systemic uptake of the copper and iron. This size of Applicant's particles is unlike any size of any commercial fixed copper, so far as the Applicant is aware of, to facilitate systemic administration. Applicant's particulate at about 90+% particulate, is about 3.5 to 1 Onm, as both a leaf pro tectorant and a systemic pesticide, and bactericide. Previous insoluble or fixed coppers are not systemic due to their large size for the most part, Applicant's particulate is about 90+% approximately 3.5 to 10 nm, in order to avoid aggregation, and has the best chance to facilitate systemic uptake. Preferably, Applicant's invention is to be administered with adjuvants/surfactants. Adjuvants ma be one or more metal chelates preferably an iron chelate. Applicant's Transmission Electron Microscopy (TEM) image of F1G.2 herein was tested with a surfactant, to insure good separation. Typical surfactants thought to be useful for the Applicant's invention are described in Silwet Adjuvants and SAG Antifoams for Agricultural Applications, (2012), published in Momentive publication of Momentive Performance Materials, Inc. of Albany, NY and "Adjuvants Products Guide" of Nufarrn.

In the foregoing description, certain terms and visual depictions are used to illustrate the preferred embodiment. However, no unnecessary limitations are to be construed by the terms used or illustrations depicted, beyond what is shown in the prior art, since the tenns and illustrations are exemplary only, and are not meant to limit the scope of the present invention. Other fixed coppers may also be, joined, doped, with iron compounds and/or zinc compounds to practice the invention. Therefore, it is understood that Applicant's use of copper hydroxide for the fixed copper compound may be readily substituted with other blown fixed copper compound. Copper hydroxide and other fixed coppers may be doped with insoluble iron compounds, and/or with insoluble zinc compounds,

Moreover, because of the ultra-small size of the doped components in the

Applicant's invention, at approximately 3.5-lOnm, it has been shown that copper hydroxide plus the adjuvant/superspreader Silwet-77 is toxic to the Asian Citrus Psyllid nymphs, a vector of bacterial HLB. Applicant's invention, with the addition of zinc (Zn) and iron

(Fe), is thought to further enhance the pesticide efficacy of copper hydroxide or other fixed coppers against this vector/carrier of HLB bacteria, and adult vector/carrier Psyllid nymphs.

The upshot is that doped components of the Applicant's invention hits the bacterial causes of HLB disease and yella fastidiosa infection of Olive Trees, and other external/internal plant pathogens in at least three ways at the least, as follows:

a) as a leaf protcctorant with supra fine particle size; i.e. 3.5-10nm;

b) as a leaf and systemic pesticide bactericide/fungicide; i.e. 3.5-1 Onm; and, c) as a pesticide against vector/carrier of HLB bacteria, the Asian Citrus Psyllid nymphs and adults, and sharpshooters, froghoppers, and spittl chugs, vectors of

XyellaFastidosa. Additionally, Applicant's invention simultaneously treats citrus canker and other citrus diseases.

In one embodiment of the present in vention, the d oped component is a fixed copper compound doped with magnesium and/or calcium.

Moreover, compounds of the Applicant's invention may be used with agents which currently synergizes with copper administ-ation, such as for example Regalia biofungicide, and horticultural oils, and pesticidal adjuvants such as Silwet-L77, and other pesticides.

Compounds of the present invention are suitable for the customary adjuvants, such as metal chelates, preferably at least one iron chelate, and formulation aids, which include but not limited to surfactants, wetting agents, stabilizers, dispersants and anti-foam agents.

Applicant claims iron compounds doped into copper hydroxide preferably iron hydroxide or iron oxide, zinc compounds doped into copper hydroxide preferably zinc oxide, iron compounds and zinc compounds doped together in copper hydroxide. These doped copper hydroxide compounds may be combined i any combination, or mixtures thereof and additional iron compounds, e.g. iron hydroxide, or zinc compounds, e.g. zinc oxide, may be added. For example, iron hydroxide doped copper hydroxide at 15 at% iron may have free unincorporated iron hydroxide with it at 5,10, 15, 20, 25, 30, 35 at% iron hydroxide or more together with the 15 at% iron hydroxide doped copper hydroxide. Applicant claims all fixed copper compounds doped with iron compounds and/or zinc compounds for agricultural pesticidal usage.

EXAMPLES

The following examples illustrate the invention without limiting it. Except where otherwise indicated, the parts and percentages shown are expressed by percentage (at%). Example 1 : Synthesis of Iron-doped Copper Hydroxide

Iron-doped Copper Hydroxide (Fe-Cu (OH)₂) composite powder is synthesized using a wet chemical process. The starting materials for the synthesis were copper nitrate hexahydrat e(Cu(>l 0_;i h .6H₂0), iron, chloride (FcCL), sodium hydroxide (NaOH) and deionized water (H₂Q). To synthesize ~15 at% Fe-doped- Cu (ΟΗ)₂, 2.43g of copper nitrate hexahydrate and 0.4 g of iron chloride were dissolved in 75 mL of deionized water in a round bottom flask. The solution was reiluxed for 3 hours. Once the solution cooled down to room temperature, 102.2 mL of 0.25 M NaOH was added. The pH at this stage was between 10 and 11 , Subsequently, the solution was transferred to a beaker and another 102.2 mL of NaOH was added, while stirring the solution, using a high shear mixer at ~1000 rpm. The pH after the addition of N aOH was between 11 and 12. The precipitate was washed, one time vising deionized water and finally dried in air. Similar methods were used to synthesize iron-copper hydroxide composite, where the amount of iron was varied from 20 to 50 at%.

FIG. 1 is an X-ray diffraction test verifying that copper hydroxide is doped with an insoluble iron. In connection therewith, in FIG.2 herein, Applicant submits a data sheet of Applicant's iron doped copper hydroxide with 15 at% iron elemental analysis of FIG.3. FIG. 2, discloses Applicant's invention tested out by TEM at about 90%+ particulates, which is a very narrow particulate distribution unlike any other commercial product, between 3.5nm and lOnm. Fig.4 is the X-ray diffraction of 15 at% Iron doped+ additional 10 at% iron that the copper hydroxide is unable to incorporate, so that the final preparation i s 15 at% Iron doped copper hydroxide+ 10 at% unincorporated/free Iron Hydroxide and or Iron Oxide.

Example 2: Synthesis of Iron-Zinc-doped Copper Hydroxide

Iron-Zinc-doped Copper Hydroxide (Fe-Zn-Cu (OH)₂) composite powder is synthesized using a wet chemical process. The starting materials for the synthesis is copper nitrate hexahydrate (Cu (N0₃)₂.6H₂0), iron chloride (FeCI₃), zinc sulfate heptahydrate (ZnS0₄.7H₂0), sodium hydroxide (NaOH) and deionized water (H₂0). To synthesize -7.5 at% Fe - 7.5 at% Zn-doped- Cu(OH)_2s 2.43g of copper nitrate hexahydrate, 0.21 g of zinc sulfate heptahydrate and 0.1 g of iron chloride is dissolved in 75 mL of deionized water in a round bottom flask. The solution is refluxed for 3 hours. Once the solution cools down to room temperature, 102.2 mL of 0.25 M NaOH is added. Subsequently, the solution is transferred to a beaker and another 102.2 mL of NaOH is added, while stimng the solution, using a high shear mixer at -1000 rpm. The precipitate is washed one time using deionized water and finally dried, in air. The similar method is used to synthesize other variations of iron-zinc-copper hydroxide composite.

Example 3: Synthesis of Zinc-doped Copper Hydroxide

[0075] Zinc-doped Copper Hydroxide (Zn-Cu(OH)₂) composite powder is synthesized using a wet chemical- process. The starting materials for the synthesis is copper nitrate hexahydrate (Οι(Ν0₃)₂.όΗ₂θ), zinc sulfate heptahydrate (ZnS0₄.7H₂0), sodium hydroxide (NaOH) and deionized water (¾0), To synthesize ~15 at% Zn-doped- Cu(OH)₂, 2.43g of copper nitrate hexahydrate, 0.42 g of zinc sulfate heptahydrate is dissolved in 75 mL of deionized water in a round bottom flask. The solution is refluxed for 3 hours. Once the solution cools down to room temperature, 102.2 mL of 0.25 M NaOH is added. Subsequently, the solution is transferred to a beaker and another 102.2 mL of NaOH is added, while stimng the solution, using a high shear mixer at ~1000 rpm. The precipitate is washed one time using deionized water and. finally is dried in air. The similar method is used to synthesize other variations of zinc-copper hydroxide composite. The Applicant preferably has used additional zinc sulfate heptahydrate, so that the finished synthesis contains zinc doped copper hydroxide plus unincorporated zinc oxide alone, in the finished preparation. Use of Compositions for treating Black Sigatoka disease, Yellow Sigatoka disease and Panama disease in banana crop plants:

The Applicant's agricultural pesticides used to treat agricultural crops affected, with vascular crop diseases such as Huang-longbing bacterial disease in orange crop plants or Xylella fasfidosa disease in olive tree crop plants, namely with zinc or iron -doped copper hydroxide singly or together, and/or with ferrites, are exquisitely also suited for fungal diseases of banana, especially the three leading banana killers worldwide, namely Black Sigatoka disease, Yellow Sigatoka disease and Panama disease. The present invention is also useful against other banana fungal diseases, including eumusae leaf spot disease, which is also of the Sigatoka complex of banana fungal diseases,

See Dunn; Matt, "Devastating plant fungus may eradicate bananas within five years", Fox News, quoting plant pathologist Ioannis Stergiopoulos, August 18, 2016, 2 pages.

According to Dunn, therefore, it would be known to those skilled in the art of banana plant science that eumusae leaf spot disease is part of the Sigatoka complex of plant fungal diseases.

Bananas are a. staple for an. excess of 400 million subsistence people in the tropics. They are under vicious critical attack by these fungal diseases. See Mourichon et al, Sigatoka Leaf Spot Diseases, PROMUSA Working Group, International Network for Improvement of Banana and Plantai n Montpellier Cedex, FR,

www.biodiversityintem.ation.al.org/viploads/tx_news/Sigatoka_leaf_spot__diseases_699.pdf, October, 1 97,which discusses Black Sigatoka disease, also known as Black Leaf Streak disease (BLSD) caused by the ascomycetous fungi Mycospliaerella fijiensis Morelet, as well as Yellow Sigatoka disease, also known as Sigatoka disease (SD), caused by the ascomycetous fungi M. musicola Leach x Mulder. Mourichon et al also discuss the fact that systemic topical spraying of fungicides is a cause of environmental concern, and that the fungi grow resistant to conventional fungicides. See Ramaker, "Banana disease continues to Spread", resource.wageningenur.nl, November 26, 2015, showing aerial photos depicting widespread destruction of banana plants in a banana plantation. Ramaker states that there is widespread destru ction, of banana plants in. the Middle East, Southeast Asia, East Africa and Australia affected by Panana disease of banana plants, Ramaker also states it is only a matter of time before it spreads to South America. Ramaker notes that efforts to quarantine afflicted banana plants have failed to stop the spread of Panama disease in banana plants.. Ramaker notes that: the Panana disease causing fungus Fusarium o sorum survives in the soil for long periods of time, even after afflicted banana plants are removed. See "Banana Cultivation" of Wageningen; see "Black Sigatoka of Banana: The most important disease of a most important fruit" of Randy Ploetz. Black Sigatoka is the most important banana disease, opus cited, see "Plant Health Australia Fact Sheet." See also Outrageous Minds publication article, "Banageddon: Millions face hunger as Panama disease and Black Sigatoka decimate global banana crop" and see also "Black Sigatoka: An Increasing Threat to Banana Cultivation" Douglas H Marin, et al.

[0078] Black Sigatoka is currently being treated with systemic fungicides which are failing. See Douglas H, Marin et al. Opus cited. In a personal communication with one of the avithors above, Turner Sutton discloses "Copper fungicides have been used for Sigatoka control, especially in the past there was a heavy use of Bordeaux mixture followed by other copper based formulations. Because of the heavy use of copper in the past it accumulates in the soil at phytotoxic levels. This, and the development of new protectant products discouraged the use of Copper fungicides. Additionally, with the introduction of agricultural oil, some copper formulations react with the oil causing phytotoxicity on the leaves." As this article discloses these systemic fungicides are becoming inactive due to mutations and chemical resistance. This has only gotten worse since the time of the article in 2003. See "Black Sigatoka" by Randy Ploetz, which discloses that fixed coppers were the old standard methods until the systemic fungicides, beginning with Benomyl.

Resistance has developed with these systemics with challenges ahead for control of Black Sigatoka disease in banana crops. According to Mourichon et al, supra, Yellow Sigatoka disease is also prone to pesticide resistance. Because of the wet conditions where bananas are grown (see Table 1, Randy Ploetz in "Disease Control"), this has necessitated up to about 50 sprayings weekly, with costly environmental damage, labor and capital intensive admini stration of these fungicides, which adds about 25% to the cost of banana production see "Top Banana" at Martinez page 5.

"Copper completes the circle for Sigatoka control" by Terry Mabbett, which discloses that "Copper fungicide has travelled the complete circle in the control of Sigatoka disease on banana. Copper was the very first fungicide to be used against yellow Sigatoka over 60 years ago and is now back in contention and favour due to its broad spectrum activity and long history of use without resistance which now plagues many single-site systemic fungicides." See Mabbett also "Copper makes a full circle" at page 221, and "Positive profile for copper fungicide" on page 221, and "Rise and fall of systemic fungicides" at page 222.

It is further noted that all existing fixed copper fungicides are "crop protectants" and do not enter the systemic vascular circul ation of the banana plant, and therefore are inactive after a banana plant has become infected. The fixed copper fungicides as crop protectants kill this Sigatoka Disease fungus spore on the leaf and block its entry through the stoma into the leaf. See Bennett et al, Black Sigatoka of Bananas and Plantains, p. 6, Cornell University , www.apsnet.org/edcenter/intropp/lessons/fungi/ascomaycetes/ Pages/BlackSigatoka.aspx, 2003.

In contrast to known fungicides, Applicant's invention with its preferred 5-1 Onm size will enter the banana leaf through the stoma, or by other means, which, is referred to being "locally systemic", to counter the fungus systemically affecting the afflicted banana plant. Simultaneously, the compositions of Applicant's invention act like other classical fixed coppers, as a crop protectant on the surface of the leaf. Moreover, the compositions of Applicant's invention is used to reduce the number of sprayings due to wet weather, because of its systemic activity like the prior systemics, which were unsuccessfully used to try and control Black Sigatoka disease or Yellow Sigatoka disease in banana plants.

Moreover, as Ploss, in U.S. Patent No. 7, 105,136B2, has shown in his Ί36 patent, inferentially, because of the systemic activity of nano copper hydroxide in using

Applicant's fungicidal compositions, only a fraction of the quantity of the previously used copper hydroxide will be needed, to be effective,

Moreover the Applicant's supercharging by doping of copper hydroxide, copper oxide or other fixed coppers, with zinc or iron, or both, further strikingly increases functional activity and reduces copper usage, thus further mitigating the harmful effects of excess copper accumulation in the soil. Moreover both the iron and zinc are recognized established micronulrients to simultaneously feed the banana, plant.

The Applicant notes that "Pesticides Registered for Use in Banana in Hawaii" of EPA reg. no. September 2, 2014, Hawaii Department of Agriculture Pesticides Branch; National Pesticide Information Retrieval System (NPiRS) Purdue University, shows ten different prior art copper hydroxide fonnulations, which were approved to treat Black and Yellow Sigatoka in bananas plus another five copper fonnulations. However, every one of those ten prior art different copper hydroxide formations, is a crop protectant with particles too big to enter the leaf site of infection, and therefore are incapable of being systemic in the vasculature of the banana plant.. In "Control of black Sigatoka disease: Challenges and. Prospects" by Ebimieowei Etebu, at page 510, Etebu states as follows: "Protectants were the first group of fungicides that were discovered and. used in crop protection. They are non systemic, and as such do not penetrate plant tissue. Protectant fungicides do not control fungi that are already established within the plant tissues since they do not penetrate the plant tissues themselves. "

The Applicant's fungicidal compositions are distinguished from the prior art by their unique doping with zinc and/or iron, singly or together, and with a unique small size, capable of entering the banana plant leaf, the site of infection.

Panama disease s another blight affecting afflicted banana plants. Panama disease is caused by a fungus Fusarium oxysporum f.sp. cubense (Foe), which, along with Black Sigatoka are the two grievous existential threats to banana crop production and the lifeblood of over 400 million subsistence banana crop fanners. Panama disease is a vascular fungus which can remain active in the soil for many decades and it cannot be fully controlled by prior art fungicides. There is a desperate need for treatment for Panama disease. See "YES WE HAVE NO BANANAS: The world's favorite fruit is slowly but surely being driven to extinction" by Gwynn Guilford in Quartz; See also "Yes! We have no bananas? It could actually happen" Mark Koba; see "Fungus Threatens Banana Supply" Mark Koba; see also "Worse Comes to Worst: Bananas and Panama. Disease-When Plant and Pathogen Clones Meet" Nadia Ordonez in PLOS Pathogens.

Applicant's fungicidal compositions, with their small size suitable for systemic uptake, in the banana plant, and the proven anti-fungal characteristics of the fixed coppers i.e. copper hydroxide, plus being doping with iron and/or zinc, make Applicant's compositions very desirable for the treatment of Panama disease.

Moreover, fixed coppers, i.e. copper hydroxide, are approved for many other banana diseases, i.e. Black pitting, see "Pesticides Registered for Use in Banana in Hawaii" (Sept. 2, 2014), In point of fact, copper hydroxide is recommended for "Bananas, Beans, Cabbage, Cacao, Carrots, Cauliflower, Celery, Citrus, Coffee, Cucumber, Grapes, Onion, Peas, Peanuts, Pepper, Potato, Rice, Tomato and Watermelon" see "Fungicides" Jocanima Corporation. In fact, copper is approved in dozens of plant diseases, bacteria, fungi and viruses, see Denis Rusjan (2012). "Copper in Horticulture, Fungicides for Plant and Animal Diseases", Dr. Dharumadurai Dhanasekaran (Ed.), pp. 257-278. However, despite the limited success of the aforementioned copper based treatments for treating banana crop plants afflicted with Black Sigatoka disease, Yellow Sigatoka disease, or Panama disease, i contrast, Applicant's doped fixed coppers compositions, which are doped with zinc, or iron, or both, or , alternatively, Applicant's ferrite compositions, are uniquely capable of systemically or effectively capable of treating banana crop plants afflicted with "Panama" disease, "Yellow Sigatoka" disease and "Black Sigatoka" disease, while minimizing the accumulation of copper in crop soils.

In conclusion, the present invention for treating banana fungal, bacterial and viral diseases includes a pesticidal, fungicidal, bactericidal, anti-pathogen or biocidal composition for use in the treatment of fungal, bacterial and viral diseases of banana plants wherei the composition comprises

a) at least one biologically inert carrier; and

b) one of i), ii) or iii) as follows:

i) at least one doped component, wherein said doped component comprises at least one fixed copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds and combinations thereof, or,

ii) at least one ferrite containing copper, zinc or iron alone, or in any combination, or, iii) at least one ferrite containing copper, zinc or iron alone, or in any combination, and at least one doped component, wherein said doped component comprises at least one fixed copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds and combinations thereof.

Although the present invention lias been disclosed in terms of a preferred embodiment, it is further known that other modifications may be made to the present invention, without departing from the spirit and scope of the invention.

Claims

I CLAIM:

1. A pesticidal, fungicidal, bactericidal, anti-pathogen or biocidal composition for use in the treatment of fungal, bacterial and viral diseases of banana plants wherein the composition comprises

a) at least one biologically inert carrier; and

b) one ofi), ii) or iii):

i) at least one doped component, wherein said doped component comprises at least one fixed, copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds and combinations tliereof, or,

ii) at least one ferrite containing copper, zinc or iron, alone, or in any combination, or, iii) at least one ferrite containing copper, zinc or iron alone, or in any combination, and at least one doped component, wherein said doped component comprises at least one fixed copper compound doped with at least one compound selected from the group consisting of iron compounds, zinc compounds and combinations thereof.

2. The composition for use according to claim 1 , wherein said fixed copper compound is selected, from the group consisting of cupric hydroxide, copper oxychloride, copper oxide, cupric carbonate basic, copper sulfate basic, tribasic copper sulfate, cuprous oxide, cupric citrate, cupric phosphate, cuprobam, indigo copper, minerals brochantite, langite, malachite, cornetite, libethenite, pseudolibemenite, pseudo-malachite, antlerite, covellite, marshite, cuprite, chalcocite, Rogojski's salt, brochantite, hydrocyanite, nantokite, dolerophane, ammonia copper carbonate, basic copper chloride, and combinations tliereof.

3. The composition of claim 2, wherein said fixed copper compound is cupric hydroxide.

4. The composition of claim 2, wherein said fixed copper compound is copper oxide.

5. The composition for use according to claim 1 , wherein said iron compounds are insoluble or substantially insoluble iron compounds.

6. The composition for use according to claim 5 wherein said iron compounds are selected from the group consisting of iron hydroxide, iron oxyhydroxide, iron oxide, iron glucose, ferric citrate, Ferritin, ferrous fumarate, ferrous sulfate, and. iron saturated human holotransferrin.

7. The composition for use according to claim 1 , wherein said zinc compounds are insoluble or substantially insoluble zinc compounds.

8. The composition for use according to claim 7, wherein said zinc compounds are selected from the group consisting of zinc hydroxide and zinc oxide.

9. The composition for use according to claim 1 , wherein said ferrite is selected from the group consisting of copper zinc iron oxide, copper iron oxide ferrite, zinc iron oxide nanopowder and copper ferrite.

10. The composition for use according to claim 1 , wherein said doped component includes about 15 at% zinc from said zinc compound doped within said fixed copper compound.

11. The composition for use according to claim 1 , wherein said doped component includes about 15 at% iron from said iron compound doped within said fixed copper compound.

12. The composition for use according to claim 1 , wherein said particle size of said doped component is about 3.5 to about 200 nm.

13. The composition of claim 12, wherein said particle size of said doped component is, preferably about 3.5 to about 10 nm.

14. The composition for use according to claim 1, wherein said doped component is only doped with an iron compound and/or a zinc compound.

15. The composition of claim 1 wherein said, doped component has a particle size of about 0.5 nm to 30 microns.

16. The composition of claim 1 wherein, said at least one ferrite has a particle size of about 0.5 nm to 30 microns.

17. The composition for use according to claim 1 , wherein the composition is administered to bacteria, fungi or viruses or their respective growth habitats by a method selected from the group consisting of dusting, sprinkling, spraying, brushing, dipping, smearing, impregnating, injection of the composition into plant vasculature, and application to a root system.

18. The composition for use according to claim. I, wherein said composition is a plant nutritional composition.

19. The composition for use according to claim 1 wherein said fungal diseases are selected from the group of Black Sigatoka disease, Yellow Sigatoka disease, leaf spot Sigatoka disease, Panama disease, Fusariiim wilt, Anthracnose, Annillaria corn rot, Black cross, Black root rot, Brown blotch, Brown spot, Ceratocystis fruit rot, Cigar-end, Cladosporium speckle, Conn, dry rot, Crown rot, Cylindrocladium root rot, Damping-off disease, Deiglitoniella fruit speckle, Diamond, spot, Dwarf Cavendish tip rot, Eyespot, Fruit freckle, Fruit rot, Fungal fruit rot, Leaf rust, Leaf speckle, Main Stark rot, Malayan leaf spot, Marasmiellus rot, Peduncle rot, Pestalotiopsis leaf spot, Phaeoscptoria leaf spot, Pitting disease, Pseudostem heart rot, Root and rhizome rot, Sclerotinia fruit rot, Septoria leaf spot, Sheath rot, Sooty mold, Speckle disease, Squirter black end disease, Stem-end rot, Trachysphaera finger rot, Tropical speckle and Verticillium tip rot,

20. The composition for use according to claim 1 wherein said bacterial diseases are selected from the group of Xanthomonas wilt, Banana bacterial wilt, Enset wilt, Blood disease, B gtok disease, gumming finger tip rot, Moko disease, Rhizome rot, and Japanese vascular wilt disease.

21. The composition for use according to cl im 1 wherein said banana viral diseases are selected f om the group of Bract mosaic disease, Banana bunchy top disease, Abaca mosaic disease, Banana streak virus disease, Badnavims, viral caused chlorosis, Cigar leaf necrosis, cucumber mosaic viras, Sugarcane bacilliform virus, and banana virus X disease.

22. The composition according to claim 1, wherein said composition further comprises at least one adjuvant.

23. The composition according to claim 22 wherein said at least one adjuvant is at least one metal chelate.

24. The composition according to claim 23 wherein said, at least one metal chelate is at least one iron chelate.