WO2017106917A3 - Reaction container and cap assembly - Google Patents

Reaction container and cap assembly Download PDF

Info

Publication number
WO2017106917A3
WO2017106917A3 PCT/AU2016/051268 AU2016051268W WO2017106917A3 WO 2017106917 A3 WO2017106917 A3 WO 2017106917A3 AU 2016051268 W AU2016051268 W AU 2016051268W WO 2017106917 A3 WO2017106917 A3 WO 2017106917A3
Authority
WO
WIPO (PCT)
Prior art keywords
reaction container
reaction
cap
barrier fluid
reagent
Prior art date
Application number
PCT/AU2016/051268
Other languages
French (fr)
Other versions
WO2017106917A2 (en
Inventor
John Corbett
SNR John CORBETT
Original Assignee
Bio Molecular Systems Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015905312A external-priority patent/AU2015905312A0/en
Application filed by Bio Molecular Systems Pty Ltd filed Critical Bio Molecular Systems Pty Ltd
Publication of WO2017106917A2 publication Critical patent/WO2017106917A2/en
Publication of WO2017106917A3 publication Critical patent/WO2017106917A3/en

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0673Handling of plugs of fluid surrounded by immiscible fluid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0689Sealing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • B01L2300/047Additional chamber, reservoir
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • B01L3/50825Closing or opening means, corks, bungs

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

The present invention relates to a reaction container that is suitable for conducting PCR. In particular, the invention relates to a pre-loaded reaction container comprising a reagent that is overlaid with a viscous barrier fluid, wherein the viscous barrier fluid seals the reagent from the atmosphere. One or more reaction reagents are added to the pre-loaded reaction container and the container is centrifuged to combine the one or more reaction reagents and the pre-loaded reagent. The pre-loaded reagent is stable at temperatures between 10 and 40°C for many months when immersed within the barrier fluid or is sealed from the atmosphere by the barrier fluid. The present invention also relates to a cap for cooperating engagement with a reaction container, the cap comprising an upper portion for receiving a sample and a lower portion adapted to sealingly engage the inlet aperture of the reaction container to thereby define a reaction chamber between the cap and the reaction container. The upper portion and the lower portion of the cap are in fluid communication by a conduit adapted to substantially retain at least a barrier fluid within the reaction chamber.
PCT/AU2016/051268 2015-12-22 2016-12-22 Reaction container and cap assembly WO2017106917A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AU2015905312A AU2015905312A0 (en) 2015-12-22 Reaction container
AU2015905312 2015-12-22
AU2016901589 2016-04-29
AU2016901589A AU2016901589A0 (en) 2016-04-29 Reaction container and cap assembly

Publications (2)

Publication Number Publication Date
WO2017106917A2 WO2017106917A2 (en) 2017-06-29
WO2017106917A3 true WO2017106917A3 (en) 2017-07-27

Family

ID=59088670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2016/051268 WO2017106917A2 (en) 2015-12-22 2016-12-22 Reaction container and cap assembly

Country Status (1)

Country Link
WO (1) WO2017106917A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6359754B1 (en) * 2017-12-29 2018-07-18 ヤマトエスロン株式会社 PCR containers and PCR containers with reagents
JP6371892B1 (en) * 2017-08-24 2018-08-08 ヤマトエスロン株式会社 Reagent cassette
CN111420622A (en) * 2020-04-30 2020-07-17 南通合瑞源生物科技有限公司 High-flux porous filter plate for nucleic acid synthesis and purification
WO2022219164A1 (en) * 2021-04-14 2022-10-20 Synhelix Tubular reaction unit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4417981A (en) * 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
US5780248A (en) * 1993-07-15 1998-07-14 Ortho Diagnostic Systems, Inc. Foil sealed cassette for agglutination reactions and liner therefor
US20020110812A1 (en) * 2001-02-12 2002-08-15 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
WO2012016720A1 (en) * 2010-08-05 2012-02-09 Vibod Gmbh New columns for incubation and isolation of chemical and/or biological samples

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4417981A (en) * 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
US5780248A (en) * 1993-07-15 1998-07-14 Ortho Diagnostic Systems, Inc. Foil sealed cassette for agglutination reactions and liner therefor
US20020110812A1 (en) * 2001-02-12 2002-08-15 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
WO2012016720A1 (en) * 2010-08-05 2012-02-09 Vibod Gmbh New columns for incubation and isolation of chemical and/or biological samples

Also Published As

Publication number Publication date
WO2017106917A2 (en) 2017-06-29

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