WO2017099487A1 - Centrifuge and centrifugation method - Google Patents

Centrifuge and centrifugation method Download PDF

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Publication number
WO2017099487A1
WO2017099487A1 PCT/KR2016/014362 KR2016014362W WO2017099487A1 WO 2017099487 A1 WO2017099487 A1 WO 2017099487A1 KR 2016014362 W KR2016014362 W KR 2016014362W WO 2017099487 A1 WO2017099487 A1 WO 2017099487A1
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WO
WIPO (PCT)
Prior art keywords
rotating chamber
storage unit
predetermined component
separated
rotation
Prior art date
Application number
PCT/KR2016/014362
Other languages
French (fr)
Korean (ko)
Inventor
이희영
양현진
배대창
Original Assignee
메디칸 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 메디칸 주식회사 filed Critical 메디칸 주식회사
Priority to JP2018530117A priority Critical patent/JP6716700B2/en
Priority to CN201680071465.3A priority patent/CN108367302B/en
Priority to US15/781,548 priority patent/US11103883B2/en
Priority claimed from KR1020160166582A external-priority patent/KR102001001B1/en
Publication of WO2017099487A1 publication Critical patent/WO2017099487A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B3/00—Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • B04B7/16—Sieves or filters

Definitions

  • the present invention relates to a centrifuge and a centrifugal separation method capable of separating a large amount of material, and more particularly, to a centrifuge and a centrifugal separation method capable of separating a predetermined material from a large amount of material using a centrifugal force and a filter. will be.
  • a centrifuge is a device that separates materials by using centrifugal force generated when an object rotates. Centrifuges can be classified into various types depending on the amount of sample to be centrifuged, the rotational speed, and the rotor. Centrifuges and centrifugation methods for separating materials can be used in a variety of fields, such as industrial, medical.
  • centrifugation is performed to separate a fluid from a material that contains a complex fluid such as blood (a liquid containing a fine solid component or a semi solid component such as jelly, a comprehensive concept of polymer or more; hereinafter referred to as 'fluid').
  • a complex fluid such as blood (a liquid containing a fine solid component or a semi solid component such as jelly, a comprehensive concept of polymer or more; hereinafter referred to as 'fluid'). Separator may be used.
  • the fluid By centrifugation, the fluid is stacked in several layers in the vessel depending on the specific gravity, and each layer thus stacked needs to be separated separately.
  • a variety of methods can be used. In general, the separation of each layer of manually stacked fluid not only requires considerable effort, but also does not guarantee the purity of the separated materials, sometimes resulting in the loss of separated materials. There was a problem.
  • the pipette is removed using a pipette to remove the layer of fluid. Some components of the liquid to be removed remain difficult to undergo the dilution process.
  • a technique of separating it mechanically is used, but it is not a technique that can be used universally. That is, such a separation method is a method that the amount of material to be separated is large enough to cover all of the inner wall of the cylindrical rotating chamber, there is a problem that it is not easy to separate the layers accumulated by centrifugation only by one-dimensional thickness. In particular, this method is not suitable when very small cell samples need to be separated.
  • the separated layers can be visually distinguished, but physically separating them is not easy.
  • Most of the liquid directly above the cell is a water component, but when the container is slowly tilted and the liquid of the upper layer is attempted to be separated, the cells of the lower layer also flow together, so that the separated cells may be lost by centrifugation.
  • the cohesion or adhesion between cells does not differ significantly from the nature of the water, so about 50% or more of the operations of separating only the liquid in the upper layer fail. Even if the separation is successful, some cells in the top layer are likely to be lost. If the centrifugal force is increased for the purpose of bringing the cells closer together, the cells may be destroyed.
  • Centrifuges used in biotechnology use centrifugal force to the extent that human cells cannot be destroyed because they must be able to separate without destroying human cells.
  • the centrifugal force allowed for centrifugation of cells is 100 G maximum and is generally lower.
  • 'G' means a gravity constant (gravitational constant), and in the centrifuge, since centrifugal force acts as a gravity force, the centrifugal force will be expressed using units of 'G'.
  • the present inventors have been registered with the above patents relating to various centrifuges and centrifugation methods for efficiently separating the desired components of the fluid from the material including the complex fluid.
  • the present inventors use the present invention to filter out the fluid from the material using a centrifugal force to a filter, and then to remove the desired component from the fluid filtered by the filter, in order to more efficiently separate the desired component in the fluid from the material including the complex fluid. It is intended to provide a centrifuge and a centrifugal separation method capable of separating only by remaining.
  • the rotation chamber which can be rotated so that the centrifugal force acts on the material contained therein;
  • a storage unit for storing a predetermined component separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber;
  • a filter arranged between the storage unit and the rotating chamber, wherein a predetermined component separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber passes to be stored in the storage unit.
  • the centrifugal separator includes a regression preventing unit that separates the material contained in the rotating chamber by the centrifugal force according to the rotation and prevents the predetermined component stored in the storage unit from returning to the rotating chamber from the storage unit.
  • the vertical cross section of the rotating chamber becomes narrower from the upper side to the lower side, and the area of the horizontal cross section of the rotating chamber may become smaller from the upper side to the lower side of the rotating chamber.
  • the storage unit may include a plurality of storage elements arranged in a position symmetrical with respect to the rotation center axis of the rotation chamber.
  • the anti-regression unit may be separated from the material contained in the rotary chamber by the centrifugal force according to the rotation of the rotary chamber, so that a component having a size smaller than a predetermined component stored in the storage unit may return to the rotary chamber from the storage unit. have.
  • the anti-regression portion may be formed as an inclined surface to make an uphill slope from the bottom of the storage portion to the upper portion connected to the rotating chamber.
  • the filter may not pass a component having a size larger than a predetermined component separated from the material contained inside the rotating chamber.
  • the filter may be adjusted to the mesh size of 1 ⁇ m to 2000 ⁇ m.
  • the filter may be adjusted to a mesh size of 10 ⁇ m to 1000 ⁇ m.
  • it may further include a cover for covering the top of the rotary chamber and a storage cover for covering the top of the storage.
  • Another embodiment of the present invention (1) the step of rotating the rotating chamber containing the material to be centrifuged so that centrifugal force acts on the material to be centrifuged; (2) increasing the centrifugal force according to the rotation of the rotating chamber to pass the material contained in the rotating chamber to the filter to separate a predetermined component and a smaller sized component from the material contained in the rotating chamber and to store it in the storage unit; (3) reducing the centrifugal force due to the rotation of the rotating chamber so that a predetermined component stored in the storage unit remains and a component having a size smaller than the predetermined component stored in the storage unit returns to the rotating chamber by gravity; And (4) discharging the predetermined components remaining in the storage unit to the outside.
  • the filter of step (2) may not pass a component of a size larger than a predetermined component to be separated from the material contained in the rotating chamber.
  • the predetermined component stored in the storage of step (3) can block the return to the rotation chamber by gravity.
  • the predetermined component remaining in the storage of step (4) may be discharged to the outside through the discharge pipe connected to the discharge port formed in the storage.
  • the centrifuge and centrifugal separation method of the present invention by separating a predetermined component from the material by the centrifugal force through the filter, and prevents the separated predetermined component from passing through the filter even if the centrifugal force smaller than the centrifugal force is applied, easily separates certain components from the material, and removes smaller components contained with the separated predetermined components by reducing the centrifugal force through the filter in reverse, and separating the predetermined components even if the centrifugal force decreases. By not passing in reverse, it has the effect that a predetermined component can be separated very easily.
  • FIG. 1 is a vertical sectional view of a centrifuge schematically showing a state in which a substance to be centrifuged is accommodated therein when the rotating chamber is stopped.
  • FIG. 2 is a vertical sectional view of the centrifuge schematically showing a cross section taken along the line II-II of FIG. 1.
  • 3 to 6 are various schematic cross-sectional views of the first storage body of the centrifuge of FIG.
  • FIG. 7 is a vertical sectional view of the centrifuge schematically showing a state in which a substance, which is the object of centrifugal separation, moves to a storage unit when the rotating chamber rotates.
  • FIG. 8 is a vertical sectional view of the centrifuge schematically showing a state in which a substance, which is the object of centrifugal separation, returns to the rotating chamber from the storage unit when the rotating chamber is stopped after rotation.
  • FIG. 9 is a vertical sectional view of the centrifuge schematically showing a state in which a predetermined component to be separated from the substance to be centrifuged at the stop after rotation of the rotating chamber remains in the storage unit.
  • FIG. 10 is a flowchart schematically illustrating a method of separating a predetermined component to be separated from a material contained in the rotating chamber by a centrifugal force according to the rotation of the rotating chamber.
  • the rotary chamber 110 may accommodate the material 10 therein and rotate the centrifugal force to act on the received material 10 to separate the material 10 contained therein by its components.
  • the material 10 accommodated inside the rotating chamber 110 to be centrifuged may be a complex fluid such as blood.
  • Such complex fluids generally contain a variety of other components in addition to the desired components to be separated.
  • the material 10 contained in the rotating chamber 110 to be centrifuged includes particles 11 to be separated and particles having a larger average radius than those 11 to be separated. It may comprise a solid phase or a liquid phase component 13 consisting of particles having a smaller average radius than the component 12 of the solid phase that is made up.
  • the rotating chamber 110 is to accommodate the material 10 therein and to rotate the centrifugal force to act on the received material 10 to separate the material 10 contained therein by its components.
  • the rotating chamber 110 allows the material 10 contained therein to sequentially move from the component receiving the greatest centrifugal force to the component receiving the smallest centrifugal force by the centrifugal force caused by the rotation of the rotating chamber 110. It is to separate by the ingredients.
  • the rotating chamber 110 may be formed in various configurations.
  • the rotation chamber 110 may include a vertical guide 111 and a horizontal guide 113, 114, as conceptually shown in FIG. 1.
  • the present invention is not limited thereto, and the rotation chamber 110 may be configured in various other configurations.
  • the rotation chamber 110 may further include a driving device for rotating the rotation chamber 110 so that the centrifugal force acts on the received material 10.
  • the rotation chamber 110 may further include a cover 112 covering the upper side of the accommodation portion 111.
  • the rotating chamber 110 may be coated with a fluorine resin such as Teflon to minimize the surface resistance when the material 10 contained therein moves. Therefore, the material 10 accommodated in the rotation chamber 110 can smoothly move the inner surface by the centrifugal force caused by the rotation of the rotation chamber 110.
  • a fluorine resin such as Teflon
  • the vertical guide part 111 accommodates the material 10 to be centrifuged and guides the received material 10 to the storage parts 121 and 122 by centrifugal force due to the rotation of the rotation chamber 110. It plays a role.
  • the vertical guide 111 may be formed in various shapes.
  • the vertical guide portion 111 may be formed such that its vertical cross section becomes narrower from the upper side to the lower side.
  • the vertical guide part 111 may be formed such that its vertical cross section forms an inverted triangle.
  • the vertical guide part 111 may have a vertical cross section of which an inverted triangular hypotenuse is convex or concave curved toward the inside or outside, or the vertical cross section of the vertical cross section forms an inverted trapezoidal shape. It may be formed.
  • the vertical guide portion 111 may be formed such that its horizontal cross section forms a circle. However, the present invention is not limited thereto, and the vertical guide part 111 may be formed such that its horizontal cross section forms various other shapes. In addition, the vertical guide portion 111 may be formed such that the area of the horizontal cross-section is smaller from the upper side to the lower side. For example, the vertical guide portion 111 may be formed such that its horizontal cross section forms a circular shape and has an inverted cone shape having an area gradually decreasing from the upper side to the lower side. As a result, the rotation chamber 110 can be prevented from significantly swinging due to the unbalance of the centrifugal force due to the high speed rotation of the rotation chamber 110.
  • the material 10 accommodated inside the rotation chamber 110 is vertically guided by gravity acting on the material 10 itself. It is arranged in the lower center of the (111).
  • the horizontal guides 113 and 114 guide the material introduced from the vertical guides 111 to the outside.
  • the horizontal guide parts 113 and 114 may be formed in various configurations.
  • the horizontal guides 113 and 114 may include the first horizontal guides 113 extending outwardly from any one of the upper edges of the vertical guides 111 and the horizontal guides 113 and 114. It may be composed of a second horizontal guide portion 114 extending toward the outside from the other portion of the upper edge of the vertical guide portion symmetrical.
  • the present invention is not limited thereto, and the horizontal guide parts 113 and 114 may extend outward from the entire upper edge of the vertical guide part 111.
  • the horizontal guides 113 and 114 may be combined to be separated from the vertical guides 111, or may be combined to be separated from the vertical guides 111.
  • the extent to which the horizontal guides 113 and 114 preferably extend to the outside may be appropriately determined in consideration of various factors such as the material to be centrifuged, the component to be separated, and the rotational speed of the rotating chamber 110. .
  • the first horizontal guide part 113 extends outwardly from any portion of the upper edge of the vertical guide part 111 to be connected to the first storage part 121. Can be.
  • the material 10 guided by the vertical guide 111 is guided to the first storage 121 connected to the first horizontal guide 113 instead of the entire upper edge of the vertical guide 111.
  • concentration the separation efficiency can be improved.
  • the first horizontal guide part 113 may have a shape for smoothly guiding the material 10 flowing from the vertical guide part 111 toward the first storage part 121.
  • the first horizontal guide part 113 is connected to the vertical guide part 111 and connected to the first storage part 121 at a portion through which the material 10 flowing from the vertical guide part 111 passes.
  • the cross-sectional area may be narrowed toward the portion through which the material 10 discharged to the storage 121 passes.
  • the first horizontal guide portion 113 is positioned so that a pair of circular arcs facing each other are positioned horizontally so that the horizontal cross section is convex outwardly and there is a gap between the pair of circular arcs.
  • the first horizontal guide part 113 may have a vertical cross section of a horizontal line.
  • the present invention is not limited thereto, and the first horizontal guide part 113 may have various other shapes.
  • the first horizontal guide portion 113 is formed such that its vertical cross-section is inclined uphill from the portion connected to the vertical guide portion 111 to the portion connected to the first storage portion 121 so that the vertical guide portion 111 is formed. The material 10 flowing from) may be smoothly guided and concentrated toward the first storage unit 121.
  • the second horizontal guide portion 114 extends outward from a portion of the upper edge of the vertical guide portion 111 that is symmetric to the first horizontal guide portion 113. 2 may be connected to the storage 122.
  • the material 10 guided by the vertical guide part 111 is guided to the second storage part 122 connected to the second horizontal guide part 114 instead of the entire upper edge of the vertical guide part 111.
  • concentration the separation efficiency can be improved.
  • the second horizontal guide part 114 may have a shape for smoothly guiding the material 10 flowing from the vertical guide part 111 toward the second storage part 122.
  • the second horizontal guide part 114 is connected to the vertical guide part 111 and is connected to the second storage part 122 at a portion where the material 10 flowing from the vertical guide part 111 passes.
  • the cross-sectional area may be narrowed toward the portion through which the material 10 discharged to the storage 122 passes.
  • the second horizontal guide portion 114 is positioned horizontally with the pair of circular arcs arranged so as to be convex outwardly and facing each other, the distance between the pair of circular arcs.
  • the second horizontal guide part 114 may have a vertical cross section formed by a horizontal line.
  • the present invention is not limited thereto, and the second horizontal guide part 114 may be formed in various other shapes.
  • the second horizontal guide portion 114 is formed such that its vertical section is inclined ascending from the portion connected to the vertical guide portion 111 to the portion connected to the second storage portion 122 so that the vertical guide portion 111 is formed. The material 10 flowing from) may be smoothly guided to the second storage part 122 to be concentrated.
  • the cover 112 covers the upper portion of the vertical guide 111.
  • the cover 112 may be formed in various configurations.
  • the cover 112 may have a shape extending to correspond to an upper portion of the vertical guide 111.
  • the cover 112 may be coupled to the vertical guide portion 111 to be detachable or not detachable.
  • the cover 112 may be an injection hole 112a for injecting the material 10 accommodated in the vertical guide 111.
  • the cover 112 may further include a portion 115 covering the upper portion of the first horizontal guide portion 131 and a portion 116 covering the upper portion of the second horizontal guide portion 132.
  • the cover 112 includes a portion 115 covering the upper portion of the first horizontal guide portion 131 and a portion 116 covering the upper portion of the second horizontal guide portion 132, respectively. It may have a shape extending to correspond to the upper portion of the upper portion and the upper portion of the second horizontal guide portion 132.
  • the storage parts 121 and 122 store the predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110.
  • the storage units 121 and 122 may be configured in various configurations.
  • the storage units 121 and 122 may include a first storage unit 121 and a second storage unit 122.
  • the storage portions 121 and 122 also have a component 13 having a smaller size than the predetermined components 11. Can be stored.
  • the component having a smaller size than the predetermined component 11 may also be stored in the storage units 121 and 122 by passing through the filters 131 and 132 described later.
  • the first storage part 121 is connected to the first horizontal guide part 113 and separated from the material 10 accommodated in the rotating chamber 110 by the centrifugal force caused by the rotation of the rotating chamber 110.
  • the predetermined component 11 guided by the first horizontal guide portion 113 via 111 is stored.
  • the first storage unit 121 may have various configurations.
  • the first storage unit 121 may include a first storage body 123 and further include a first storage cover 124.
  • the first storage body 123 is separated from the material 10 accommodated inside the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110 and the first horizontal guide portion 113 via the vertical guide portion 111.
  • the predetermined component 11 guided by to this end, the first storage body 123 may have a concave shape in which a predetermined component 11 separated from the material 10 accommodated in the rotating chamber 110 may be stored.
  • the first storage body 123 has a vertical cross-section narrowing from the upper side to the lower side, and the horizontal cross-section has a circular shape. Can be.
  • the present invention is not limited thereto, and the first storage body 123 may have various other configurations. Other various shapes of the horizontal cross section of the first storage body 123 are shown in FIGS. 3 to 6 by way of example.
  • the first storage body 123 has a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110. It is desirable to have a size that can be stored after being stored.
  • the first storage body 123 includes a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110, and the predetermined component 11. It is preferred that the component 13 having a smaller size is smaller than the extent to which the rotating chamber 110 can be stored together after stopping. As a result, the component 13 having a size smaller than the predetermined component 11 is formed from the first storage body 123 from the time when the centrifugal force due to the rotation of the rotating chamber 110 becomes smaller than the predetermined level to the point at which the rotary chamber 110 is not operated. It is possible to easily return to the inside of the rotating chamber 110 by moving away from the), it is possible to significantly improve the separation efficiency of the separated predetermined component (11).
  • the first storage body 123 is a predetermined component 11 is separated from the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110, the first storage body 123 It is preferable to include a first regression preventing portion (123a) to prevent the return to the rotating chamber 110 in the.
  • the predetermined component 11 separated from the material 10 accommodated in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 and stored in the first storage body 123 is formed of the rotation chamber 110.
  • the first regression preventing part 123a is uphill from a bottom part such as the center of the first storage body 123 to a part connected to the first horizontal guide part 113. It may be formed as an inclined surface forming an inclination. The inclination degree, length, shape, etc. of the inclined surface forming the first regression preventing part 123a may be appropriately adjusted according to the kind and characteristics of the predetermined component 11 stored in the first storage body 123.
  • the first regression preventing part 123a enhances the effect of preventing the regression of the predetermined component 11 stored in the first storage body 123 into the rotation chamber 110.
  • the first storage body 123 may be used.
  • the effect of suppressing the regression of the smaller component 13 into the rotating chamber 110 stored together with the predetermined component 11 is also high, which is undesirable.
  • the first regression preventing part 123a may increase the effect of preventing the regression of the predetermined component 11 stored in the first storage body 123 into the rotation chamber 110 while being predetermined in the first storage body 123. It is desirable to appropriately adjust the size, shape, and the like so that the effect of suppressing the return of the smaller component 13 stored with the component 11 into the rotation chamber 110 is lowered.
  • the components that do not pass through the first filter 131 of the material 10 contained in the rotation chamber 110 ( 12 does not move to the first storage body 123 and remains in the rotating chamber 110, and the predetermined component 11 to be separated passes through the first filter 131 to the first storage body 123.
  • the component 13 which is not a separation target but smaller in specific gravity or size than the predetermined component 11 to be separated also moves through the first filter 131 to the first storage body 123.
  • the predetermined component to be separated by moving to the first storage body 123 11 may be arranged in the lower layer, and the component 13 having a specific gravity or size smaller than the predetermined component 11 may be arranged in the upper layer.
  • the component 13 having a specific gravity smaller or smaller than the predetermined component 11 to be separated is returned without being disturbed by the first regression preventing portion 123a and rotated through the first filter 131.
  • the predetermined component 11 to be separated is prevented from returning by being disturbed by the first regression preventing portion 123a and thus not reaching the first filter 131.
  • the ratio of the predetermined component 11 to be separated is increased, so that the separation efficiency can be greatly improved.
  • the first storage cover 124 covers the upper portion of the first storage body 123.
  • the first storage cover 124 may be formed in various configurations.
  • the first storage cover 124 may have a shape extending to correspond to an upper portion of the first storage body 123.
  • the first storage cover 124 may be coupled to the first storage body 123 to be separated or not separated.
  • a first outlet 124a may be added to the first storage cover 124 to discharge the predetermined component 11 to be separated stored in the first storage body 123.
  • a connection pipe such as a hose
  • the predetermined component 11 to be separated can be easily discharged to the outside in an uncontaminated state.
  • a pump or a storage tank are not required separately, the structure of the device can be simplified.
  • the first storage cover 124 may be coupled to or separated from the portion 115 that covers the upper portion of the first horizontal guide 131.
  • the second storage part 122 is connected to the second horizontal guide part 114 and separated from the material 10 accommodated inside the rotating chamber 110 by centrifugal force due to the rotation of the rotating chamber 110.
  • the predetermined component 11 guided by the second horizontal guide portion 114 via 111 is stored.
  • the second storage unit 122 may be configured in various configurations.
  • the second storage unit 122 may include a second storage body 125 and further include a second storage cover 126.
  • the second storage body 125 is separated from the material 10 contained in the rotating chamber 110 by the centrifugal force caused by the rotation of the rotating chamber 110, and the second horizontal guide portion 114 via the vertical guide portion 111.
  • the predetermined component 11 guided by To this end, the second storage body 125 may have a concave shape in which a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 may be stored.
  • the second storage body 125 has a shape in which the vertical cross section thereof becomes narrower from the upper side to the lower side, and the horizontal cross section has a circular shape, and thus has an inverse conical shape as a whole. Can be.
  • the present invention is not limited thereto, and the second storage body 125 may have various other configurations.
  • Other various shapes of the horizontal cross section of the second storage body 125 may be formed in a shape similar to other various shapes of the horizontal cross section of the first storage body 123 illustrated in FIGS. 3 to 6.
  • the second storage body 125 has a predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by at least centrifugal force due to the rotation of the rotation chamber 110, and the rotation chamber 110 is stopped. It is desirable to have a size that can be stored after being stored.
  • the second storage body 125 includes a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110, and the predetermined component 11. It is preferred that the component 13 having a smaller size is smaller than the extent to which the rotating chamber 110 can be stored together after stopping. As a result, the second storage body 125 has a component 13 having a size smaller than the predetermined component 11 from the time when the centrifugal force due to the rotation of the rotating chamber 110 becomes smaller than the predetermined level to the point at which the rotary chamber 110 does not work. It is possible to easily return to the inside of the rotating chamber 110 by moving away from the), it is possible to significantly improve the separation efficiency of the separated predetermined component (11).
  • the second storage body 125 has a predetermined component 11 separated and stored in the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110. It is preferable to include a second regression preventing portion (125a) to prevent the return to the rotating chamber (110).
  • the predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 and stored in the second storage body 125 is formed of the rotation chamber 110.
  • the gravity acts more than the centrifugal force, thereby leaving the second storage body 125 and attempting to return to the rotation chamber 110.
  • the second regression preventing part 125a may ascend from a bottom part such as the center of the second storage body 125 to a part connected to the second horizontal guide part 114. It may be formed as an inclined surface forming an inclination. The inclination degree, length, shape, etc. of the inclined surface forming the second regression preventing part 125a can be appropriately adjusted according to the kind and characteristics of the predetermined component 11 stored in the second storage body 125.
  • the second regression preventing part 125a enhances the effect of preventing the regression of the predetermined component 11 stored in the second storage body 125 into the rotation chamber 110.
  • the second storage body 125 The effect of suppressing the regression of the smaller component 13 into the rotating chamber 110 stored together with the predetermined component 11 is also high, which is undesirable.
  • the second regression preventing part 125a has a predetermined effect on the second storage body 125 while increasing the effect of preventing the return of the predetermined component 11 stored in the second storage body 125 to the rotation chamber 110. It is desirable to appropriately adjust the size, shape, and the like so that the effect of suppressing the return of the smaller component 13 stored with the component 11 into the rotation chamber 110 is lowered.
  • the component 10 that has not passed through the second filter 132 of the material 10 contained in the rotating chamber 110 ( 12 does not move to the second storage body 125 and remains in the rotating chamber 110, and the predetermined component 11 to be separated passes through the second filter 132 to the second storage body 125.
  • the component 13 which is not a separation target but smaller in specific gravity or size than the predetermined component 11 to be separated also moves through the second filter 132 to the second storage body 125.
  • a predetermined component to be separated by moving to the second storage body 125 11 may be arranged in the lower layer, and the component 13 having a specific gravity or size smaller than the predetermined component 11 may be arranged in the upper layer.
  • the component 13 having a specific gravity smaller or smaller than the predetermined component 11 to be separated is returned without being disturbed by the second regression preventing portion 125a to rotate through the second filter 132.
  • the predetermined component 11 to be separated is prevented from returning by being disturbed by the second regression preventing portion 125a and thus not reaching the second filter 131.
  • the ratio of the predetermined component 11 to be separated is increased, so that the separation efficiency can be greatly improved.
  • the second storage cover 126 covers the upper portion of the second storage body 125.
  • the second storage cover 126 may be formed in various configurations.
  • the second storage cover 126 may have a shape extending to correspond to an upper portion of the second storage body 125.
  • the second storage cover 126 may be coupled to the second storage body 125 to be separated or not separated.
  • a second outlet 126a may be added to the second storage cover 126 to discharge the predetermined component 11 to be separated stored in the second storage body 125.
  • a connection pipe such as a hose may be connected to the second outlet 126a to easily discharge the predetermined component 11 stored therein without being contaminated. In this case, since a pump or a storage tank are not required separately, the structure of the device can be simplified.
  • the second storage cover 126 may be combined to be separated from or separated from the portion 115 covering the upper portion of the second horizontal guide 132.
  • the filters 131 and 132 pass through a predetermined component 11 separated from the material 10 contained in the rotary chamber 110 by centrifugal force due to the rotation of the rotary chamber 110.
  • the filters 131 and 132 may be formed in various configurations.
  • the filters 131 and 132 may include a first filter 131 and a second filter 132.
  • the present invention is not limited thereto, and the filters 131 and 132 may be formed of one filter or three or more filters.
  • the first filter 131 may be arranged between the rotation chamber 110 and the first storage 121.
  • the first filter 131 may include a first horizontal guide 113 between the vertical guide 111 and the first storage 121 of the rotating chamber 110. ) Can be arranged on.
  • the first filter 131 is a predetermined material which is to be separated without moving all of the material 10 contained in the rotating chamber 110 toward the first storage part 121 by the centrifugal force due to the rotation of the rotating chamber 110.
  • the component 12 having a specific gravity or size larger than that of the component 11 can be filtered out.
  • the present invention is not limited thereto, and the first filter 131 may be arranged at an arbitrary position between the rotation chamber 110 and the first storage 121.
  • the first filter 131 passes a predetermined component 11 or a smaller size or specific gravity component 13 to be separated from the material 10 contained in the rotary chamber 110 and passes the predetermined component 11.
  • Component 12 of greater size or specific gravity is not passed through.
  • the first filter 131 is accommodated in the rotating chamber 110 and contained in blood 10 (blood corpuscle), plasma, free oil and stem cells and other impurities contained in the material 10 to be centrifuged.
  • blood 10 blood corpuscle
  • plasma free oil and stem cells and other impurities contained in the material 10 to be centrifuged.
  • the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity are passed, but the component 12 having a relatively larger size or specific gravity, such as a solid close to a sphere, is not passed.
  • the first filter 131 may adjust the size of the mesh in a predetermined range according to the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110.
  • the mesh size of the first filter 131 may generally be adjusted to 1 ⁇ m to 2000 ⁇ m.
  • the mesh size of the first filter 131 may be adjusted to 10 ⁇ m to 1000 ⁇ m. desirable.
  • the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 may be filtered out. It becomes possible.
  • the first filter 131 can appropriately adjust the area of the cross section according to the centrifugation speed, the centrifugation yield, or the like. For example, the area of the cross section of the first filter 131 may be increased to increase the centrifugation speed, or the area of the cross section of the first filter 131 may be reduced to increase the centrifugation yield. However, the present invention is not limited thereto, and the area of the cross section of the first filter 131 may be variously adjusted.
  • the second filter 132 may be arranged between the rotation chamber 110 and the second storage unit 122.
  • the second filter 132 may include a second horizontal guide portion 114 between the vertical guide portion 111 and the second storage portion 122 of the rotating chamber 110. ) Can be arranged on.
  • the second filter 132 may be separated from the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110 without moving to the second storage part 122.
  • the component 12 having a specific gravity or size larger than that of the component 11 can be filtered out.
  • the present invention is not limited thereto, and the second filter 132 may be arranged at an arbitrary position between the rotation chamber 110 and the second storage unit 122.
  • the second filter 132 passes through a predetermined component 11 or a component 13 having a smaller size or specific gravity to be separated from the material 10 contained in the rotary chamber 110 and the predetermined component 11.
  • Component 12 of greater size or specific gravity is not passed through.
  • the second filter 132 is accommodated in the rotating chamber 110 and contained in blood 10 (blood corpuscle), plasma, free oil and stem cells and other impurities contained in the material 10 to be centrifuged.
  • blood 10 blood corpuscle
  • plasma free oil and stem cells and other impurities contained in the material 10 to be centrifuged.
  • the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity are passed, but the component 12 having a relatively larger size or specific gravity, such as a solid close to a sphere, is not passed.
  • the second filter 132 may adjust the size of the mesh in a predetermined range according to the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110.
  • the mesh size of the second filter 132 may generally be adjusted to 1 ⁇ m to 2000 ⁇ m.
  • the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 is a stem cell
  • the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 may be filtered out. It becomes possible.
  • the second filter 132 can appropriately adjust the area of the cross section according to the centrifugation speed, centrifugation yield, or the like.
  • the area of the cross section of the second filter 132 may be increased to increase the centrifugation speed, or the area of the cross section of the second filter 132 may be reduced to increase the centrifugation yield.
  • the present invention is not limited thereto, and the area of the cross section of the second filter 132 may be variously adjusted.
  • the rotation chamber 110 starts to rotate, the material 10 to be subjected to the centrifugal separation accommodated inside the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 is vertically guided by the rotation chamber 110. It starts to move from bottom to top along the inner surface of the part 111.
  • the material 10 to be subjected to the centrifugal separation accommodated inside the rotating chamber 110 is exemplarily shown in FIG. 7.
  • the first and second horizontal guides 113 and 114 of the rotary chamber 110 are guided by the first and second horizontal guides 113 and 114 to pass through the first and second filters 131 and 132.
  • second storage units 121 and 122 the predetermined component 11 to be separated and the smaller component 13 having a smaller size or specific gravity from the material 10 to be subjected to centrifugal separation accommodated inside the rotating chamber 110 may include the first and second filters 131.
  • the first and second reservoirs 121 and 122 are moved through the 132, but the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated may be the first and second. It cannot pass through the filters 131 and 132 and thus cannot be moved to the first and second storage units 121 and 122.
  • the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity than that of the component 13 are larger than the storage capacity of the first and second storage units 121 and 122. Only a part of the first and second storage units 121 and 122 move.
  • the predetermined component 11 to be separated is arranged in a lower layer of the first and second storage parts 121 and 122, and the component 13 having a smaller size or a non-bore than the predetermined component 11 to be separated is formed. It is arranged on the upper layer of the first and second storage (121) 122.
  • the predetermined component 11 to be separated and moved to the first and second storage portions 121 and 122 and the like are more than that.
  • the smaller size or specific gravity component 13 will have a greater gravity effect. Accordingly, as illustrated in FIG. 8, the component 13 having a smaller size or specific gravity than the predetermined component 11 to be separated is arranged on the upper layers of the first and second storage portions 121 and 122. Is returned to the rotation chamber 110 in the first and second storage (121, 122).
  • the first and second regression prevention operations are performed. It is smoothly returned to the rotation chamber 110 without being disturbed by the parts 123a and 125a.
  • the predetermined components 11 to be separated arranged in the lower layers of the first and second storage parts 121 and 122 may have a greater influence of gravity than the centrifugal force, but the first and second regression preventing parts 123a. Return to the rotating chamber 110 is prevented by the 125a and remains in the first and second storage parts 121 and 122 as they are.
  • the first and second storage parts 121 and 122 Remains, and no component 13 of a size or specific gravity smaller than the predetermined component 11 remains. Thereafter, the predetermined components 11 to be separated in the first and second storage parts 121 and 122 are discharged to the outside through the first and second discharge ports 124a and 126a. Alternatively, before discharging the predetermined component 11 to be separated remaining in the first and second storage parts 121 and 122 to the outside, the substance 10 to be centrifuged is added to the rotating chamber 110. By repeating the centrifugation process by injecting into, the content of the predetermined component 11 to be separated in the first and second storage parts 121 and 122 may be increased.
  • the centrifugal separation method 200 rotates a rotating chamber containing a material to be centrifuged so that centrifugal force acts on the material to be centrifuged.
  • Step 210 When injecting the material to be centrifuged into the rotating chamber, the cover provided in the injection port of the rotary chamber can be opened and the material can be injected, or the material can be injected using an injection tube connected to the injection port.
  • the material contained in the rotating chamber moves from the lower side to the upper side along the inclined inner surface of the rotating chamber and passes through the filter provided at the upper outer side to reach the storage unit. At this time, a component having a size larger than the predetermined component does not pass through the filter.
  • Predetermined components are arranged in the upper layer of the storage portion, and smaller components are arranged in the lower layer of the storage portion.
  • the centrifugal force according to the rotation of the rotating chamber is reduced so that a predetermined component stored in the storage unit remains and a component having a smaller size than the predetermined component stored in the storage unit returns to the rotating chamber by gravity (230). do.
  • the predetermined component stored in the storage unit is blocked from returning to the rotation chamber by gravity.
  • a regression prevention unit may be formed in the storage unit to block a path in which a predetermined component returns to the rotation chamber by gravity.
  • step 240 of discharging the predetermined component remaining in the storage unit to the outside This discharge may be through a hose connected to an outlet formed in the reservoir.
  • the present invention can be used in a centrifuge and a centrifugal separation method capable of separating a large amount of material.

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Abstract

One embodiment of the present invention relates to a centrifuge comprising: a rotary chamber capable of rotating so that a centrifugal force acts on a substance contained therein; a storage unit for storing a predetermined component separated from the substance contained in the rotary chamber by the centrifugal force due to the rotation of the rotary chamber; and a filter which is arranged between the storage unit and the rotary chamber and through which the predetermined component separated from the substance contained in the rotary chamber by the centrifugal force due to the rotation of the rotary chamber passes to be stored in the storage unit, wherein the storage unit includes a reversion prevention unit for preventing the predetermined component, which is separated from the substance contained in the rotary chamber by the centrifugal force due to the rotation of the rotary chamber and stored in the storage unit, from returning from the storage unit to the rotary chamber. As such, the present invention has an effect capable of very easily separating a predetermined component sought to be separated.

Description

원심분리기 및 원심분리방법Centrifuge and Centrifugal Method
본 발명은 대용량의 물질을 분리할 수 있는 원심분리기 및 원심분리방법에 관한 것으로서, 상세하게는 원심력과 필터를 이용하여 대용량의 물질로부터 소정의 물질을 분리할 수 있는 원심분리기 및 원심분리방법에 관한 것이다.The present invention relates to a centrifuge and a centrifugal separation method capable of separating a large amount of material, and more particularly, to a centrifuge and a centrifugal separation method capable of separating a predetermined material from a large amount of material using a centrifugal force and a filter. will be.
원심분리기(centrifuge)는 물체가 회전할 때 발생하는 원심력을 이용하여 물질을 분리하는 장치이다. 원심분리기는 원심분리를 하는 시료의 양, 회전 속도, 로터 등에 따라 여러 가지 형태로 구분할 수 있다. 물질을 분리하기 위한 원심분리기 및 원심분리방법은 산업, 의료 등 다양한 분야에서 사용될 수 있다.A centrifuge is a device that separates materials by using centrifugal force generated when an object rotates. Centrifuges can be classified into various types depending on the amount of sample to be centrifuged, the rotational speed, and the rotor. Centrifuges and centrifugation methods for separating materials can be used in a variety of fields, such as industrial, medical.
예컨대, 생명공학실험실에서 혈액과 같은 복합적 유체(미세한 고형성분 또는 젤리와 같은 반고형성분을 포함하는 액체로서 고분자 이상의 포괄적 개념; 이하 '유체'라 칭한다)를 포함하는 물질에서 유체를 분리하기 위하여 원심분리기를 사용할 수 있다.For example, in a biotechnology laboratory, centrifugation is performed to separate a fluid from a material that contains a complex fluid such as blood (a liquid containing a fine solid component or a semi solid component such as jelly, a comprehensive concept of polymer or more; hereinafter referred to as 'fluid'). Separator may be used.
원심분리기에 의해 유체는 용기 내에서 비중에 따라 여러 개의 층으로 적층되는데, 이와 같이 적층된 각 층을 별도로 분리할 필요가 있다. 이를 위하여 다양한 방법을 이용할 수 있는데, 일반적으로 수작업으로 적층된 유체의 각 층을 분리할 경우에는, 상당한 수고가 필요할 뿐만 아니라 분리된 물질들의 순도를 보장할 수 없고, 때로는 분리된 물질의 손실이 발생하는 문제점이 있었다. 다른 방법으로 피펫(pipette)을 이용하여 적층된 유체의 위층을 제거하고 있는데, 제거하고자 하는 액체의 성분 일부가 남아 희석공정을 거쳐야 하는 어려움이 있었다.By centrifugation, the fluid is stacked in several layers in the vessel depending on the specific gravity, and each layer thus stacked needs to be separated separately. To this end, a variety of methods can be used. In general, the separation of each layer of manually stacked fluid not only requires considerable effort, but also does not guarantee the purity of the separated materials, sometimes resulting in the loss of separated materials. There was a problem. Alternatively, the pipette is removed using a pipette to remove the layer of fluid. Some components of the liquid to be removed remain difficult to undergo the dilution process.
이와 같은 문제점을 해결하기 위한 시도로서, 원통형 회전챔버가 회전함에 따라 비중이 가장 높은 물질이 회전챔버의 외곽에 쌓이면 이를 기계적으로 분리하는 기술이 사용되기도 하였으나 범용으로 사용될 수 있는 기술은 아니다. 즉 이와 같은 분리법은 분리되는 물질의 양이 원통형 회전챔버의 내벽을 모두 덮을 정도로 많아야 가능한 방법이며, 원심분리되어 누적된 층들을 1차원적 두께만으로 구분하는 작업도 용이하지 않은 문제점이 있었다. 특히, 이러한 방법은 극소량의 세포 샘플을 분리해야 하는 경우에는 적합하지 않다.In an attempt to solve such a problem, as the cylindrical rotating chamber rotates, if a material having the highest specific gravity accumulates on the outer side of the rotating chamber, a technique of separating it mechanically is used, but it is not a technique that can be used universally. That is, such a separation method is a method that the amount of material to be separated is large enough to cover all of the inner wall of the cylindrical rotating chamber, there is a problem that it is not easy to separate the layers accumulated by centrifugation only by one-dimensional thickness. In particular, this method is not suitable when very small cell samples need to be separated.
세포가 원심분리기에 의해 분리된 경우, 분리된 층들을 시각적으로는 구분할 수 있지만 이를 물리적으로 분리하는 작업은 쉽지 않다. 세포 바로 위층의 액체는 대부분 물 성분인데, 용기를 서서히 기울이며 위층의 액체를 분리해 내려고 하면 가장 아래층의 세포도 함께 유동하기 때문에 원심분리에 의해 분리된 세포가 유실될 수 있는 문제가 있다. 원심력이 작용하지 않는 상태에서는 세포들 사이의 응집력 또는 부착력이 물의 성질과 크게 차이가 나지 않기 때문에 위층의 액체만을 분리하는 작업의 약 50% 이상이 실패한다. 분리작업이 성공하는 경우에도 최상층의 일부 세포들이 유실되기가 십상이다. 만일 세포들 사이를 더욱 밀착시키기 위한 목적으로 원심력을 증가시킨다면 세포는 파괴될 수도 있다.When cells are separated by centrifuges, the separated layers can be visually distinguished, but physically separating them is not easy. Most of the liquid directly above the cell is a water component, but when the container is slowly tilted and the liquid of the upper layer is attempted to be separated, the cells of the lower layer also flow together, so that the separated cells may be lost by centrifugation. In the absence of centrifugal force, the cohesion or adhesion between cells does not differ significantly from the nature of the water, so about 50% or more of the operations of separating only the liquid in the upper layer fail. Even if the separation is successful, some cells in the top layer are likely to be lost. If the centrifugal force is increased for the purpose of bringing the cells closer together, the cells may be destroyed.
생명공학에 사용되는 원심분리기는 인체 세포를 파괴하지 않고 분리할 수 있어야 하기 때문에 인체 세포가 파괴되지 않을 정도의 원심력을 이용한다. 세포의 원심분리에 허용되는 원심력은 100G가 최대이고 일반적으로는 이보다 낮다. 여기에서 'G'는 중력상수(gravitational constant)를 의미하는 것으로, 통상적으로 원심분리기에서는 원심력이 중력과 같은 힘으로 작용하므로 'G'의 단위를 사용하여 원심력을 표현하기로 한다.Centrifuges used in biotechnology use centrifugal force to the extent that human cells cannot be destroyed because they must be able to separate without destroying human cells. The centrifugal force allowed for centrifugation of cells is 100 G maximum and is generally lower. Here, 'G' means a gravity constant (gravitational constant), and in the centrifuge, since centrifugal force acts as a gravity force, the centrifugal force will be expressed using units of 'G'.
위와 같이 원심분리기를 사용하여 복합적 유체를 포함한 물질에서 유체를 용기 내에 적층된 상태로 분리시키고 적층된 유체 중에서 원하는 성분을 더욱 효율적으로 분리하기 위한 방법 및 수단을 강구할 필요가 있다.As described above, it is necessary to devise a method and means for separating a fluid in a stacked state in a container using a centrifugal separator and separating a desired component from the stacked fluid more efficiently.
본 발명자는 복합적 유체를 포함한 물질에서 유체 중의 원하는 성분을 효율적으로 분리하기 위한 다양한 원심분리기 및 원심분리방법에 관한 위와 같은 특허로 등록받았다.The present inventors have been registered with the above patents relating to various centrifuges and centrifugation methods for efficiently separating the desired components of the fluid from the material including the complex fluid.
나아가, 본 발명자는 본 발명을 통해, 복합적 유체를 포함한 물질에서 유체 중의 원하는 성분을 더욱 효율적으로 분리하기 위하여, 원심력을 이용하여 물질로부터 유체를 필터로 걸러낸 다음, 필터에 의해 걸러진 유체 중에서 원하는 성분만을 잔류시켜 분리할 수 있는 원심분리기 및 원심분리방법을 제공하고자 한다.Furthermore, the present inventors use the present invention to filter out the fluid from the material using a centrifugal force to a filter, and then to remove the desired component from the fluid filtered by the filter, in order to more efficiently separate the desired component in the fluid from the material including the complex fluid. It is intended to provide a centrifuge and a centrifugal separation method capable of separating only by remaining.
본 발명의 일 실시례는, 내부에 수용된 물질에 원심력이 작용하도록 회전할 수 있는 회전챔버; 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분이 저장되는 저장부; 및 저장부와 회전챔버 사이에 배열되며, 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분이 저장부에 저장되기 위해 통과하는 필터를 구비하고, 저장부는 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리되어 저장부에 저장된 소정의 성분이 저장부에서 회전챔버로 회귀하지 않게 하는 회귀방지부를 포함하는 원심분리기이다.One embodiment of the invention, the rotation chamber which can be rotated so that the centrifugal force acts on the material contained therein; A storage unit for storing a predetermined component separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber; And a filter arranged between the storage unit and the rotating chamber, wherein a predetermined component separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber passes to be stored in the storage unit. The centrifugal separator includes a regression preventing unit that separates the material contained in the rotating chamber by the centrifugal force according to the rotation and prevents the predetermined component stored in the storage unit from returning to the rotating chamber from the storage unit.
본 실시례에 있어서, 회전챔버의 수직단면은 상측에서 하측으로 갈수록 좁아지고 회전챔버의 수평단면의 면적은 회전챔버의 상측에서 하측으로 갈수록 작아질 수 있다.In this embodiment, the vertical cross section of the rotating chamber becomes narrower from the upper side to the lower side, and the area of the horizontal cross section of the rotating chamber may become smaller from the upper side to the lower side of the rotating chamber.
본 실시례에 있어서, 저장부는 회전챔버의 회전중심축을 기준으로 대칭이 되는 위치에 배열되는 복수의 저장요소들을 포함할 수 있다.In the present embodiment, the storage unit may include a plurality of storage elements arranged in a position symmetrical with respect to the rotation center axis of the rotation chamber.
본 실시례에 있어서, 회귀방지부는 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리되어 저장부에 저장된 소정의 성분보다 작은 크기의 성분은 저장부에서 회전챔버로 회귀하게 할 수 있다.In the present embodiment, the anti-regression unit may be separated from the material contained in the rotary chamber by the centrifugal force according to the rotation of the rotary chamber, so that a component having a size smaller than a predetermined component stored in the storage unit may return to the rotary chamber from the storage unit. have.
본 실시례에 있어서, 회귀방지부는 저장부의 바닥에서 회전챔버에 연결되는 상부까지 오르막 경사를 이루는 경사면으로 형성되어 있을 수 있다.In the present embodiment, the anti-regression portion may be formed as an inclined surface to make an uphill slope from the bottom of the storage portion to the upper portion connected to the rotating chamber.
본 실시례에 있어서, 필터는 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분보다 큰 크기의 성분은 통과시키지 않을 수 있다.In the present embodiment, the filter may not pass a component having a size larger than a predetermined component separated from the material contained inside the rotating chamber.
본 실시례에 있어서, 필터는 메쉬의 크기가 1μm 내지 2000μm로 조절되어 있을 수 있다. In the present embodiment, the filter may be adjusted to the mesh size of 1μm to 2000μm.
본 실시례에 있어서, 필터는 메쉬의 크기가 10μm 내지 1000μm로 조절되어 있을 수 있다.In this embodiment, the filter may be adjusted to a mesh size of 10μm to 1000μm.
본 실시례에 있어서, 회전챔버의 상부를 커버하는 덮개와 저장부의 상부를 커버하는 저장덮개를 더 구비할 수 있다.In the present embodiment, it may further include a cover for covering the top of the rotary chamber and a storage cover for covering the top of the storage.
본 발명의 다른 실시례는, (1) 원심분리의 대상이 되는 물질에 원심력이 작용하도록 원심분리의 대상이 되는 물질을 수용하는 회전챔버를 회전시키는 단계; (2) 회전챔버의 회전에 따른 원심력을 증가시켜 회전챔버에 수용된 물질을 필터에 통과시킴으로써 회전챔버에 수용된 물질에서 소정의 성분과 그보다 작은 크기의 성분을 분리하여 저장부에 저장하는 단계; (3) 회전챔버의 회전에 따른 원심력을 감소시켜 저장부에 저장된 소정의 성분은 잔존시키고 저장부에 저장된 소정의 성분보다 작은 크기의 성분은 중력에 의하여 회전챔버로 회귀시키는 단계; 및 (4) 저장부에 잔존하는 소정의 성분을 외부로 배출하는 단계를 포함하는 원심분리방법이다.Another embodiment of the present invention, (1) the step of rotating the rotating chamber containing the material to be centrifuged so that centrifugal force acts on the material to be centrifuged; (2) increasing the centrifugal force according to the rotation of the rotating chamber to pass the material contained in the rotating chamber to the filter to separate a predetermined component and a smaller sized component from the material contained in the rotating chamber and to store it in the storage unit; (3) reducing the centrifugal force due to the rotation of the rotating chamber so that a predetermined component stored in the storage unit remains and a component having a size smaller than the predetermined component stored in the storage unit returns to the rotating chamber by gravity; And (4) discharging the predetermined components remaining in the storage unit to the outside.
본 실시례에 있어서, (2) 단계의 필터는 회전챔버에 수용된 물질 중에서 분리하고자 하는 소정의 성분보다 큰 크기의 성분을 통과시키지 않을 수 있다.In the present embodiment, the filter of step (2) may not pass a component of a size larger than a predetermined component to be separated from the material contained in the rotating chamber.
본 실시례에 있어서, (3) 단계의 저장부에 저장된 소정의 성분은 중력에 의하여 회전챔버로 회귀하는 것을 차단할 수 있다.In this embodiment, the predetermined component stored in the storage of step (3) can block the return to the rotation chamber by gravity.
본 실시례에 있어서, (4) 단계의 저장부에 잔존하는 소정의 성분은 저장부에 형성된 배출구에 연결된 배출관을 통해 외부로 배출될 수 있다.In this embodiment, the predetermined component remaining in the storage of step (4) may be discharged to the outside through the discharge pipe connected to the discharge port formed in the storage.
본 발명의 원심분리기 및 원심분리방법은, 물질 중에서 소정의 성분을 원심력에 의해 필터를 통과시켜 분리하고, 분리된 소정의 성분이 그 원심력보다 작은 원심력이 작용하더라도 필터를 역으로 통과하지 않도록 함으로써, 물질 중에서 소정의 성분을 용이하게 분리하고, 분리된 소정의 성분과 함께 포함된 더 작은 크기의 성분은 원심력을 줄여서 필터를 역으로 통과시켜 제거하고, 분리된 소정의 성분은 원심력이 줄어들더라도 필터를 역으로 통과하지 않도록 하여, 소정의 성분을 매우 용이하게 분리해 낼 수 있는 효과를 갖는다.In the centrifuge and centrifugal separation method of the present invention, by separating a predetermined component from the material by the centrifugal force through the filter, and prevents the separated predetermined component from passing through the filter even if the centrifugal force smaller than the centrifugal force is applied, Easily separates certain components from the material, and removes smaller components contained with the separated predetermined components by reducing the centrifugal force through the filter in reverse, and separating the predetermined components even if the centrifugal force decreases. By not passing in reverse, it has the effect that a predetermined component can be separated very easily.
도 1은 회전챔버의 정지시에 그 내부에 원심분리의 대상이 되는 물질이 수용된 상태를 개략적으로 나타내는 원심분리기의 수직단면도이다.1 is a vertical sectional view of a centrifuge schematically showing a state in which a substance to be centrifuged is accommodated therein when the rotating chamber is stopped.
도 2는 도 1의 II-II 선을 따라 이루어진 단면을 개략적으로 나타내는 원심분리기의 수직단면도이다. FIG. 2 is a vertical sectional view of the centrifuge schematically showing a cross section taken along the line II-II of FIG. 1.
도 3 내지 도 6은 도 1의 원심분리기의 제1 저장본체의 수평단면을 다양하게 개략적으로 나타내는 도면들이다.3 to 6 are various schematic cross-sectional views of the first storage body of the centrifuge of FIG.
도 7은 회전챔버의 회전시에 그 내부에 원심분리의 대상이 되는 물질이 저장부로 이동한 상태를 개략적으로 나타내는 원심분리기의 수직단면도이다.FIG. 7 is a vertical sectional view of the centrifuge schematically showing a state in which a substance, which is the object of centrifugal separation, moves to a storage unit when the rotating chamber rotates.
도 8은 회전챔버의 회전 후 정지시에 그 내부에 원심분리의 대상이 되는 물질이 저장부에서 회전챔버로 회귀하는 상태를 개략적으로 나타내는 원심분리기의 수직단면도이다.FIG. 8 is a vertical sectional view of the centrifuge schematically showing a state in which a substance, which is the object of centrifugal separation, returns to the rotating chamber from the storage unit when the rotating chamber is stopped after rotation.
도 9은 회전챔버의 회전 후 정지시에 원심분리의 대상이 되는 물질에서 분리하고자 하는 소정의 성분이 저장부에 잔존하는 상태를 개략적으로 나타내는 원심분리기의 수직단면도이다.FIG. 9 is a vertical sectional view of the centrifuge schematically showing a state in which a predetermined component to be separated from the substance to be centrifuged at the stop after rotation of the rotating chamber remains in the storage unit.
도 10은 회전챔버의 회전에 따른 원심력에 의하여 회전챔버 내부에 수용된 물질에서 분리하고자 하는 소정의 성분을 분리하는 방법을 개략적으로 나타내는 순서도이다.10 is a flowchart schematically illustrating a method of separating a predetermined component to be separated from a material contained in the rotating chamber by a centrifugal force according to the rotation of the rotating chamber.
발명을 실시하기 위한 구체적인 내용을 실시례에 기초하여 설명한다. 이러한 실시례는 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 실시하기 위한 구체적인 내용을 이해할 수 있도록 하기 위하여 예시적으로 제공되는 것으로서 여러 가지 다른 형태로 변형될 수 있으므로, 본 발명의 범위가 이하의 실시례에 의해 한정되는 것은 아니다.EMBODIMENT OF THE INVENTION The concrete content for implementing this invention is demonstrated based on an Example. Such embodiments are provided by way of example so that those skilled in the art can understand the specific contents for carrying out the invention can be modified in many different forms, the scope of the present invention is It is not limited by the following example.
(실시례 1)(Example 1)
본 실시례는, 예시적으로 도 1에 나타나 있는 바와 같이, 원심분리기(100)로서 회전챔버(110), 제1 저장부(121)와 제2 저장부(122) 및 제1 필터(131)와 제2 필터(132)를 구비한다.In this embodiment, as shown in FIG. 1 by way of example, as the centrifuge 100, the rotary chamber 110, the first storage unit 121 and the second storage unit 122 and the first filter 131 And a second filter 132.
회전챔버(110)는 내부에 물질(10)을 수용할 수 있고 수용된 물질(10)에 원심력이 작용하도록 회전함으로써 내부에 수용된 물질(10)을 그 성분별로 분리할 수 있는 것이다. 회전챔버(110) 내부에 수용되어 원심분리 대상이 되는 물질(10)은 혈액과 같은 복합적 유체일 수 있다. 이러한 복합적 유체는 일반적으로 분리하고자 하는 소정의 성분 이외에 다양한 다른 성분을 포함하고 있다. 예컨대, 회전챔버(110) 내부에 수용되어 원심분리 대상이 되는 물질(10)은 분리하고자 하는 성분(11)을 포함하고 있음은 물론 분리하고자 하는 성분(11)보다 더 큰 평균 반경을 갖는 입자로 이루어진 고상(solid phase)의 성분(12)과 분리하고자 하는 성분(11)보다 더 작은 평균 반경을 갖는 입자로 이루어진 고상 또는 액상(liquid phase)의 성분(13)을 포함할 수 있다.The rotary chamber 110 may accommodate the material 10 therein and rotate the centrifugal force to act on the received material 10 to separate the material 10 contained therein by its components. The material 10 accommodated inside the rotating chamber 110 to be centrifuged may be a complex fluid such as blood. Such complex fluids generally contain a variety of other components in addition to the desired components to be separated. For example, the material 10 contained in the rotating chamber 110 to be centrifuged includes particles 11 to be separated and particles having a larger average radius than those 11 to be separated. It may comprise a solid phase or a liquid phase component 13 consisting of particles having a smaller average radius than the component 12 of the solid phase that is made up.
회전챔버(110)는 내부에 물질(10)을 수용하고 수용된 물질(10)에 원심력이 작용하도록 회전함으로써 내부에 수용된 물질(10)을 그 성분별로 분리할 수 있도록 하는 것이다. 회전챔버(110)는 그 내부에 수용된 물질(10)이 회전챔버(110)의 회전에 따른 원심력에 의하여 원심력을 가장 크게 받는 성분부터 원심력을 가장 작게 받는 성분까지 순차적으로 이동하게 함으로써 물질(10)을 그 성분별로 분리하는 것이다. 이를 위하여 회전챔버(110)는 다양한 구성으로 이루어질 수 있다. 예컨대, 회전챔버(110)는, 예시적으로 도 1에 개념적으로 나타나 있는 바와 같이, 수직안내부(111)와 수평안내부(113)(114)를 구비할 수 있다. 다만, 이에 한정되지 않고 회전챔버(110)는 다른 다양한 구성으로 이루어질 수 있다. 또한, 회전챔버(110)는, 도 1에는 나타나 있지 않지만, 수용된 물질(10)에 원심력이 작용하도록 회전챔버(110)를 회전시키는 구동장치를 더 구비할 수 있다. 그밖에 회전챔버(110)는 수용부(111)의 상측을 덮는 덮개(112)를 더 포함할 수 있다. The rotating chamber 110 is to accommodate the material 10 therein and to rotate the centrifugal force to act on the received material 10 to separate the material 10 contained therein by its components. The rotating chamber 110 allows the material 10 contained therein to sequentially move from the component receiving the greatest centrifugal force to the component receiving the smallest centrifugal force by the centrifugal force caused by the rotation of the rotating chamber 110. It is to separate by the ingredients. To this end, the rotating chamber 110 may be formed in various configurations. For example, the rotation chamber 110 may include a vertical guide 111 and a horizontal guide 113, 114, as conceptually shown in FIG. 1. However, the present invention is not limited thereto, and the rotation chamber 110 may be configured in various other configurations. In addition, although not shown in FIG. 1, the rotation chamber 110 may further include a driving device for rotating the rotation chamber 110 so that the centrifugal force acts on the received material 10. In addition, the rotation chamber 110 may further include a cover 112 covering the upper side of the accommodation portion 111.
회전챔버(110)는 내부에 수용된 물질(10)이 이동할 때의 표면저항을 최소화하기 위하여 내측 표면을 테프론과 같은 불소수지로 코팅되도록 할 수 있다. 따라서 회전챔버(110) 내에 수용된 물질(10)이 회전챔버(110)의 회전에 따른 원심력에 의하여 내측 표면을 원활하게 이동할 수 있게 된다. The rotating chamber 110 may be coated with a fluorine resin such as Teflon to minimize the surface resistance when the material 10 contained therein moves. Therefore, the material 10 accommodated in the rotation chamber 110 can smoothly move the inner surface by the centrifugal force caused by the rotation of the rotation chamber 110.
수직안내부(111)는 원심분리의 대상이 되는 물질(10)을 수용하고 수용된 물질(10)이 회전챔버(110)의 회전에 따른 원심력에 의해 저장부(121)(122)로 이동하도록 안내하는 역할을 한다. 이를 위하여 수직안내부(111)는 다양한 형상으로 이루어질 수 있다. 예컨대, 수직안내부(111)는 그 수직단면이 상측에서 하측으로 갈수록 좁아지는 형상을 이루도록 형성될 수 있다. 수직안내부(111)는, 예시적으로 도 1에 나타나 있는 바와 같이, 그 수직단면이 역삼각형을 이루도록 형성될 수 있다. 다만, 이에 한정되지 않으며, 수직안내부(111)는 그 수직단면이 역삼각형의 빗변이 내부 또는 외부를 향하여 볼록 또는 오목하게 굽어져 있는 형상을 이루거나 또는 그 수직단면이 역사다리꼴의 형상을 이루도록 형성될 수도 있다.The vertical guide part 111 accommodates the material 10 to be centrifuged and guides the received material 10 to the storage parts 121 and 122 by centrifugal force due to the rotation of the rotation chamber 110. It plays a role. For this purpose, the vertical guide 111 may be formed in various shapes. For example, the vertical guide portion 111 may be formed such that its vertical cross section becomes narrower from the upper side to the lower side. As illustrated in FIG. 1, the vertical guide part 111 may be formed such that its vertical cross section forms an inverted triangle. However, the present invention is not limited thereto, and the vertical guide part 111 may have a vertical cross section of which an inverted triangular hypotenuse is convex or concave curved toward the inside or outside, or the vertical cross section of the vertical cross section forms an inverted trapezoidal shape. It may be formed.
수직안내부(111)는 그 수평단면이 원형을 이루도록 형성될 수 있다. 다만, 이에 한정되지 않고 수직안내부(111)는 그 수평단면이 다른 다양한 형상을 이루도록 형성될 수도 있다. 또한, 수직안내부(111)는 상측에서 하측으로 갈수록 그 수평단면의 면적이 작아지도록 형성될 수 있다. 예컨대, 수직안내부(111)는 그 수평단면이 원형을 이루고 상측에서 하측으로 갈수록 점차 작아지는 면적을 갖는 역원뿔 형상을 이루도록 형성될 수 있다. 이에 의하여 회전챔버(110)의 고속 회전에 따른 원심력의 불균형으로 인해 회전챔버(110)가 요동하는 것을 대폭 방지할 수 있게 된다. The vertical guide portion 111 may be formed such that its horizontal cross section forms a circle. However, the present invention is not limited thereto, and the vertical guide part 111 may be formed such that its horizontal cross section forms various other shapes. In addition, the vertical guide portion 111 may be formed such that the area of the horizontal cross-section is smaller from the upper side to the lower side. For example, the vertical guide portion 111 may be formed such that its horizontal cross section forms a circular shape and has an inverted cone shape having an area gradually decreasing from the upper side to the lower side. As a result, the rotation chamber 110 can be prevented from significantly swinging due to the unbalance of the centrifugal force due to the high speed rotation of the rotation chamber 110.
회전챔버(110)가 정지되어 있는 경우에는, 예시적으로 도 1에 나타나 있는 바와 같이, 회전챔버(110) 내부에 수용된 물질(10)은 물질(10) 자체에 작용하는 중력에 의하여 수직안내부(111)의 하측 중심에 배열되게 된다. When the rotation chamber 110 is stopped, as illustrated in FIG. 1, the material 10 accommodated inside the rotation chamber 110 is vertically guided by gravity acting on the material 10 itself. It is arranged in the lower center of the (111).
수평안내부(113)(114)는 수직안내부(111)로부터 유입되는 물질을 외부 쪽으로 안내하는 것이다. 이를 위하여 수평안내부(113)(114)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1에 나타나 있는 바와 같이 수평안내부(113)(114)는 수직안내부(111)의 상부 가장자리 중 어느 한 부분으로부터 외부 쪽으로 연장되는 제1 수평안내부(113)와 이에 대칭을 이루는 수직안내부(111)의 상부 가장자리의 다른 한 부분으로부터 외부 쪽으로 연장되는 제2 수평안내부(114)로 이루어질 수 있다. 다만, 이에 한정되지 않고 수평안내부(113)(114)는 수직안내부(111)의 상부 가장자리 전체로부터 외부 쪽으로 연장될 수도 있다. 이러한 수평안내부(113)(114)는 수직안내부(111)에 분리할 수 없도록 결합할 수도 있으며 수직안내부(111)에 분리할 수 있도록 결합할 수도 있다. 수평안내부(113)(114)가 외부 쪽으로 어느 정도 연장되는 것이 바람직한 것인지는 원심분리 대상이 되는 물질, 분리하고자 하는 성분, 회전챔버(110)의 회전속도 등 다양한 요소를 고려하여 적절하게 정할 수 있다.The horizontal guides 113 and 114 guide the material introduced from the vertical guides 111 to the outside. For this purpose, the horizontal guide parts 113 and 114 may be formed in various configurations. For example, as illustrated in FIG. 1, the horizontal guides 113 and 114 may include the first horizontal guides 113 extending outwardly from any one of the upper edges of the vertical guides 111 and the horizontal guides 113 and 114. It may be composed of a second horizontal guide portion 114 extending toward the outside from the other portion of the upper edge of the vertical guide portion symmetrical. However, the present invention is not limited thereto, and the horizontal guide parts 113 and 114 may extend outward from the entire upper edge of the vertical guide part 111. The horizontal guides 113 and 114 may be combined to be separated from the vertical guides 111, or may be combined to be separated from the vertical guides 111. The extent to which the horizontal guides 113 and 114 preferably extend to the outside may be appropriately determined in consideration of various factors such as the material to be centrifuged, the component to be separated, and the rotational speed of the rotating chamber 110. .
제1 수평안내부(113)는, 예시적으로 도 2에 나타나 있는 바와 같이, 수직안내부(111)의 상부 가장자리의 어느 한 부분으로부터 외부 쪽으로 연장되어 제1 저장부(121)에 연결되도록 이루어질 수 있다. 이에 의하여 수직안내부(111)에 의해 안내되는 물질(10)이 수직안내부(111)의 상부 가장자리 전체가 아닌 제1 수평안내부(113)에 연결되는 제1 저장부(121)로 안내되어 집중됨으로써 분리효율을 향상시킬 수 있다. As illustrated in FIG. 2, the first horizontal guide part 113 extends outwardly from any portion of the upper edge of the vertical guide part 111 to be connected to the first storage part 121. Can be. As a result, the material 10 guided by the vertical guide 111 is guided to the first storage 121 connected to the first horizontal guide 113 instead of the entire upper edge of the vertical guide 111. By concentration, the separation efficiency can be improved.
제1 수평안내부(113)는 수직안내부(111)로부터 유입되는 물질(10)을 제1 저장부(121) 쪽으로 원활하게 안내하도록 하기 위한 형상으로 이루어질 수 있다. 이를 위하여 제1 수평안내부(113)는 수직안내부(111)에 연결되어 수직안내부(111)로부터 유입되는 물질(10)이 통과하는 부분에서 제1 저장부(121)에 연결되어 제1 저장부(121)로 배출되는 물질(10)이 통과하는 부분으로 갈수록 횡단면적이 좁아지도록 형성될 수 있다. 예컨대, 제1 수평안내부(113)는, 예시적으로 도 2에 나타나 있는 바와 같이, 그 수평단면은 외부로 볼록하도록 배열된 한 쌍의 원호가 서로 마주보면서 위치하고 이러한 한 쌍의 원호 사이의 간격은 수직안내부(111)에 연결되는 부분에서 제1 저장부(121)에 연결되는 부분으로 갈수록 좁아지도록 형성될 수 있다. 또한, 제1 수평안내부(113)는, 예시적으로 도 1에 나타나 있는 바와 같이, 그 수직단면이 수평선으로 이루어질 수 있다. 다만, 이에 한정되지 않고 제1 수평안내부(113)는 다른 다양한 형상으로 이루어질 수 있다. 예컨대, 제1 수평안내부(113)는 그 수직단면이 수직안내부(111)에 연결되는 부분에서 제1 저장부(121)에 연결되는 부분으로 갈수록 오르막 경사를 이루도록 형성됨으로써 수직안내부(111)로부터 유입되는 물질(10)을 제1 저장부(121) 쪽으로 원활하게 안내하여 집중되도록 할 수 있다. The first horizontal guide part 113 may have a shape for smoothly guiding the material 10 flowing from the vertical guide part 111 toward the first storage part 121. To this end, the first horizontal guide part 113 is connected to the vertical guide part 111 and connected to the first storage part 121 at a portion through which the material 10 flowing from the vertical guide part 111 passes. The cross-sectional area may be narrowed toward the portion through which the material 10 discharged to the storage 121 passes. For example, as illustrated in FIG. 2, the first horizontal guide portion 113 is positioned so that a pair of circular arcs facing each other are positioned horizontally so that the horizontal cross section is convex outwardly and there is a gap between the pair of circular arcs. May be formed to be narrower from the portion connected to the vertical guide portion 111 to the portion connected to the first storage portion 121. In addition, as illustrated in FIG. 1, the first horizontal guide part 113 may have a vertical cross section of a horizontal line. However, the present invention is not limited thereto, and the first horizontal guide part 113 may have various other shapes. For example, the first horizontal guide portion 113 is formed such that its vertical cross-section is inclined uphill from the portion connected to the vertical guide portion 111 to the portion connected to the first storage portion 121 so that the vertical guide portion 111 is formed. The material 10 flowing from) may be smoothly guided and concentrated toward the first storage unit 121.
제2 수평안내부(114)는, 예시적으로 도 2에 나타나 있는 바와 같이, 수직안내부(111)의 상부 가장자리 중에서 제1 수평안내부(113)와 대칭이 되는 부분으로부터 외부 쪽으로 연장되어 제2 저장부(122)에 연결되도록 이루어질 수 있다. 이에 의하여 수직안내부(111)에 의해 안내되는 물질(10)이 수직안내부(111)의 상부 가장자리 전체가 아닌 제2 수평안내부(114)에 연결되는 제2 저장부(122)로 안내되어 집중됨으로써 분리효율을 향상시킬 수 있다. As illustrated in FIG. 2, the second horizontal guide portion 114 extends outward from a portion of the upper edge of the vertical guide portion 111 that is symmetric to the first horizontal guide portion 113. 2 may be connected to the storage 122. As a result, the material 10 guided by the vertical guide part 111 is guided to the second storage part 122 connected to the second horizontal guide part 114 instead of the entire upper edge of the vertical guide part 111. By concentration, the separation efficiency can be improved.
제2 수평안내부(114)는 수직안내부(111)로부터 유입되는 물질(10)을 제2 저장부(122) 쪽으로 원활하게 안내하도록 하기 위한 형상으로 이루어질 수 있다. 이를 위하여 제2 수평안내부(114)는 수직안내부(111)에 연결되어 수직안내부(111)로부터 유입되는 물질(10)이 통과하는 부분에서 제2 저장부(122)에 연결되어 제2 저장부(122)로 배출되는 물질(10)이 통과하는 부분으로 갈수록 횡단면적이 좁아지도록 형성될 수 있다. 예컨대, 제2 수평안내부(114)는, 예시적으로 도 2에 나타나 있는 바와 같이, 그 수평단면은 외부로 볼록하도록 배열된 한 쌍의 원호가 서로 마주보면서 위치하고 이러한 한 쌍의 원호 사이의 간격은 수직안내부(111)에 연결되는 부분에서 제2 저장부(122)에 연결되는 부분으로 갈수록 좁아지도록 형성될 수 있다. 또한, 제2 수평안내부(114)는, 예시적으로 도 1에 나타나 있는 바와 같이, 그 수직단면이 수평선으로 이루어질 수 있다. 다만, 이에 한정되지 않고 제2 수평안내부(114)는 다른 다양한 형상으로 이루어질 수 있다. 예컨대, 제2 수평안내부(114)는 그 수직단면이 수직안내부(111)에 연결되는 부분에서 제2 저장부(122)에 연결되는 부분으로 갈수록 오르막 경사를 이루도록 형성됨으로써 수직안내부(111)로부터 유입되는 물질(10)을 제2 저장부(122) 쪽으로 원활하게 안내하여 집중되도록 할 수 있다. The second horizontal guide part 114 may have a shape for smoothly guiding the material 10 flowing from the vertical guide part 111 toward the second storage part 122. To this end, the second horizontal guide part 114 is connected to the vertical guide part 111 and is connected to the second storage part 122 at a portion where the material 10 flowing from the vertical guide part 111 passes. The cross-sectional area may be narrowed toward the portion through which the material 10 discharged to the storage 122 passes. For example, as shown in FIG. 2, the second horizontal guide portion 114 is positioned horizontally with the pair of circular arcs arranged so as to be convex outwardly and facing each other, the distance between the pair of circular arcs. May be formed to be narrower from the portion connected to the vertical guide portion 111 to the portion connected to the second storage portion 122. In addition, as illustrated in FIG. 1, the second horizontal guide part 114 may have a vertical cross section formed by a horizontal line. However, the present invention is not limited thereto, and the second horizontal guide part 114 may be formed in various other shapes. For example, the second horizontal guide portion 114 is formed such that its vertical section is inclined ascending from the portion connected to the vertical guide portion 111 to the portion connected to the second storage portion 122 so that the vertical guide portion 111 is formed. The material 10 flowing from) may be smoothly guided to the second storage part 122 to be concentrated.
덮개(112)는 수직안내부(111)의 상부를 커버하는 것이다. 이를 위하여 덮개(112)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1에 나타나 있는 바와 같이 덮개(112)는 수직안내부(111)의 상부에 대응하도록 연장되는 형상으로 이루어질 수 있다. 이러한 덮개(112)는 수직안내부(111)에 분리할 수 있도록 또는 분리할 수 없도록 결합할 수 있다. 또한 덮개(112)에는 수직안내부(111)에 수용되는 물질(10)을 주입하기 위한 주입구(112a)가 추가될 수 있다.The cover 112 covers the upper portion of the vertical guide 111. To this end, the cover 112 may be formed in various configurations. For example, as illustrated in FIG. 1, the cover 112 may have a shape extending to correspond to an upper portion of the vertical guide 111. The cover 112 may be coupled to the vertical guide portion 111 to be detachable or not detachable. In addition, the cover 112 may be an injection hole 112a for injecting the material 10 accommodated in the vertical guide 111.
덮개(112)는 제1 수평안내부(131)의 상부를 커버하는 부분(115)과 제2 수평안내부(132)의 상부를 커버하는 부분(116)을 더 포함할 수 있다. 덮개(112)는 제1 수평안내부(131)의 상부를 커버하는 부분(115)과 제2 수평안내부(132)의 상부를 커버하는 부분(116)은 각각 제1 수평안내부(131)의 상부와 제2 수평안내부(132)의 상부에 대응하도록 연장되는 형상으로 이루어질 수 있다.The cover 112 may further include a portion 115 covering the upper portion of the first horizontal guide portion 131 and a portion 116 covering the upper portion of the second horizontal guide portion 132. The cover 112 includes a portion 115 covering the upper portion of the first horizontal guide portion 131 and a portion 116 covering the upper portion of the second horizontal guide portion 132, respectively. It may have a shape extending to correspond to the upper portion of the upper portion and the upper portion of the second horizontal guide portion 132.
저장부(121)(122)는 회전챔버(110)의 회전에 따른 원심력에 의해 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)을 저장하는 것이다. 이를 위하여 저장부(121)(122)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1에 나타나 있는 바와 같이 저장부(121)(122)는 제1 저장부(121)와 제2 저장부(122)로 이루어질 수 있다. 저장부(121)(122)에는 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11) 이외에 이러한 소정의 성분(11)보다 더 작은 크기를 갖는 성분(13)도 저장될 수 있다. 회전챔버(110)의 회전에 따른 원심력에 의해 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11) 이외에 이러한 소정의 성분(11)보다 더 작은 크기를 갖는 성분(13)도 후술하는 필터(131)(132)를 통과하여 저장부(121)(122)에 저장될 수 있기 때문이다.The storage parts 121 and 122 store the predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110. To this end, the storage units 121 and 122 may be configured in various configurations. For example, as illustrated in FIG. 1, the storage units 121 and 122 may include a first storage unit 121 and a second storage unit 122. In addition to the predetermined components 11 to be separated from the material 10 contained inside the rotating chamber 110, the storage portions 121 and 122 also have a component 13 having a smaller size than the predetermined components 11. Can be stored. In addition to the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110, the component having a smaller size than the predetermined component 11 ( 13 may also be stored in the storage units 121 and 122 by passing through the filters 131 and 132 described later.
제1 저장부(121)는 제1 수평안내부(113)에 연결되어 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되고 수직안내부(111)를 거쳐 제1 수평안내부(113)에 의해 안내되는 소정의 성분(11)을 저장하는 것이다. 이를 위하여 제1 저장부(121)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1 및 도 2에 나타나 있는 바와 같이 제1 저장부(121)는 제1 저장본체(123)를 구비하며 제1 저장덮개(124)를 더 구비할 수 있다.The first storage part 121 is connected to the first horizontal guide part 113 and separated from the material 10 accommodated in the rotating chamber 110 by the centrifugal force caused by the rotation of the rotating chamber 110. The predetermined component 11 guided by the first horizontal guide portion 113 via 111 is stored. To this end, the first storage unit 121 may have various configurations. For example, as illustrated in FIGS. 1 and 2, the first storage unit 121 may include a first storage body 123 and further include a first storage cover 124.
제1 저장본체(123)는 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되고 수직안내부(111)를 거쳐 제1 수평안내부(113)에 의해 안내되는 소정의 성분(11)을 저장하는 것이다. 이를 위하여 제1 저장본체(123)는 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)이 저장될 수 있는 오목한 형상으로 이루어질 수 있다. 예컨대, 예시적으로 도 1 및 도 2에 나타나 있는 바와 같이 제1 저장본체(123)는 그 수직단면이 상측에서 하측으로 갈수록 좁아지는 형상이고 그 수평단면이 원형의 형상으로 이루어짐으로써 전체적으로 역원뿔 형상일 수 있다. 다만, 이에 한정되지 않고 제1 저장본체(123)는 다른 다양한 구성으로 이루어질 수도 있다. 제1 저장본체(123)의 수평단면의 다른 다양한 형상이 도 3 내지 도 6에 예시적으로 나타나 있다. The first storage body 123 is separated from the material 10 accommodated inside the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110 and the first horizontal guide portion 113 via the vertical guide portion 111. The predetermined component 11 guided by To this end, the first storage body 123 may have a concave shape in which a predetermined component 11 separated from the material 10 accommodated in the rotating chamber 110 may be stored. For example, as illustrated in FIGS. 1 and 2, the first storage body 123 has a vertical cross-section narrowing from the upper side to the lower side, and the horizontal cross-section has a circular shape. Can be. However, the present invention is not limited thereto, and the first storage body 123 may have various other configurations. Other various shapes of the horizontal cross section of the first storage body 123 are shown in FIGS. 3 to 6 by way of example.
제1 저장본체(123)는 적어도 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)이 회전챔버(110)가 정지된 후 저장될 수 있는 정도의 크기를 갖는 것이 바람직하다. The first storage body 123 has a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110. It is desirable to have a size that can be stored after being stored.
제1 저장본체(123)는 적어도 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)과 이러한 소정의 성분(11)보다 작은 크기를 갖는 성분(13)이 회전챔버(110)가 정지된 후 함께 저장될 수 있는 정도보다 작은 크기를 갖는 것이 바람직하다. 이에 의하여, 회전챔버(110)의 회전에 따른 원심력이 소정의 수준보다 작게 되는 시점부터 작용하지 않게 되는 시점까지, 소정의 성분(11)보다 작은 크기의 성분(13)이 제1 저장본체(123)로부터 이탈하여 회전챔버(110) 내부로 쉽게 회귀할 수 있게 됨으로써, 분리된 소정의 성분(11)의 분리효율을 대폭 향상시킬 수 있게 된다.The first storage body 123 includes a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110, and the predetermined component 11. It is preferred that the component 13 having a smaller size is smaller than the extent to which the rotating chamber 110 can be stored together after stopping. As a result, the component 13 having a size smaller than the predetermined component 11 is formed from the first storage body 123 from the time when the centrifugal force due to the rotation of the rotating chamber 110 becomes smaller than the predetermined level to the point at which the rotary chamber 110 is not operated. It is possible to easily return to the inside of the rotating chamber 110 by moving away from the), it is possible to significantly improve the separation efficiency of the separated predetermined component (11).
제1 저장본체(123)는 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되어 저장된 소정의 성분(11)이 제1 저장본체(123)에서 회전챔버(110)로 회귀하지 않게 하는 제1 회귀방지부(123a)를 포함하는 것이 바람직하다. 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되어 제1 저장본체(123)에 저장된 소정의 성분(11)은, 회전챔버(110)의 회전에 따른 원심력이 작아지거나 작용하지 않게 되면, 원심력보다 중력이 더 크게 작용하게 되어 제1 저장본체(123)에서 이탈하여 회전챔버(110)로 회귀하려고 한다. 이 경우 제1 회귀방지부(123a)에 의하여 제1 저장본체(123)에 저장된 소정의 성분(11)이 회전챔버(110)로 회귀하는 것을 방지할 수 있게 됨으로써 분리효율을 향상시킬 수 있게 된다. 이러한 제1 회귀방지부(123a)는, 예시적으로 도 1에 나타나 있는 바와 같이, 제1 저장본체(123)의 중심과 같은 바닥 부분에서 제1 수평안내부(113)에 연결되는 부분까지 오르막 경사를 이루는 경사면으로 형성될 수 있다. 제1 회귀방지부(123a)를 형성하는 경사면의 경사진 정도나 길이, 형상 등은 제1 저장본체(123)에 저장된 소정의 성분(11)의 종류 및 특성 등에 의하여 적절하게 조절할 수 있다.The first storage body 123 is a predetermined component 11 is separated from the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110, the first storage body 123 It is preferable to include a first regression preventing portion (123a) to prevent the return to the rotating chamber 110 in the. The predetermined component 11 separated from the material 10 accommodated in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 and stored in the first storage body 123 is formed of the rotation chamber 110. When the centrifugal force due to the rotation becomes small or does not work, the gravity acts more than the centrifugal force, thereby leaving the first storage body 123 and attempting to return to the rotation chamber 110. In this case, it is possible to prevent the predetermined component 11 stored in the first storage body 123 from returning to the rotation chamber 110 by the first regression preventing unit 123a, thereby improving separation efficiency. . As shown in FIG. 1, the first regression preventing part 123a is uphill from a bottom part such as the center of the first storage body 123 to a part connected to the first horizontal guide part 113. It may be formed as an inclined surface forming an inclination. The inclination degree, length, shape, etc. of the inclined surface forming the first regression preventing part 123a may be appropriately adjusted according to the kind and characteristics of the predetermined component 11 stored in the first storage body 123.
제1 회귀방지부(123a)는 제1 저장본체(123)에 저장된 소정의 성분(11)의 회전챔버(110)로의 회귀를 방지하는 효과를 높이는 것이 바람직하나, 이 경우 제1 저장본체(123)에 소정의 성분(11)과 함께 저장된 그보다 작은 성분(13)의 회전챔버(110)로의 회귀를 억제하는 효과도 함께 높아져서 바람직하지 않게 된다. 따라서 제1 회귀방지부(123a)는 제1 저장본체(123)에 저장된 소정의 성분(11)의 회전챔버(110)로의 회귀를 방지하는 효과는 높이면서 제1 저장본체(123)에 소정의 성분(11)과 함께 저장된 그보다 작은 성분(13)의 회전챔버(110)로의 회귀를 억제하는 효과는 낮추도록 그 크기나 형상 등을 적절하게 조절하는 것이 바람직하다.Preferably, the first regression preventing part 123a enhances the effect of preventing the regression of the predetermined component 11 stored in the first storage body 123 into the rotation chamber 110. In this case, the first storage body 123 may be used. The effect of suppressing the regression of the smaller component 13 into the rotating chamber 110 stored together with the predetermined component 11 is also high, which is undesirable. Accordingly, the first regression preventing part 123a may increase the effect of preventing the regression of the predetermined component 11 stored in the first storage body 123 into the rotation chamber 110 while being predetermined in the first storage body 123. It is desirable to appropriately adjust the size, shape, and the like so that the effect of suppressing the return of the smaller component 13 stored with the component 11 into the rotation chamber 110 is lowered.
회전챔버(110)의 회전에 따른 원심력이 작용하게 되면, 예시적으로 도 7에 나타나 있는 바와 같이, 회전챔버(110)에 수용된 물질(10) 중에서 제1 필터(131)를 통과하지 못한 성분(12)은 제1 저장본체(123)으로 이동하지 못하여 회전챔버(110)에 남게 되고, 분리하고자 하는 소정의 성분(11)은 제1 필터(131)를 통과하여 제1 저장본체(123)로 이동하게 된다. 그러나 이 경우 분리대상은 아니지만 분리하고자 하는 소정의 성분(11)보다 비중 또는 크기가 더 작은 성분(13)도 제1 필터(131)를 통과하여 제1 저장본체(123)로 이동하게 된다. When the centrifugal force due to the rotation of the rotation chamber 110 is applied, as shown in FIG. 7, the components that do not pass through the first filter 131 of the material 10 contained in the rotation chamber 110 ( 12 does not move to the first storage body 123 and remains in the rotating chamber 110, and the predetermined component 11 to be separated passes through the first filter 131 to the first storage body 123. Will move. However, in this case, the component 13 which is not a separation target but smaller in specific gravity or size than the predetermined component 11 to be separated also moves through the first filter 131 to the first storage body 123.
회전챔버(110)의 회전에 따른 원심력이 소정의 수준보다 작아지거나 작용하지 않게 되면, 예시적으로 도 8에 나타나 있는 바와 같이, 제1 저장본체(123)로 이동한 분리하고자 하는 소정의 성분(11)은 하층에 배열되고 소정의 성분(11)보다 비중이나 크기가 더 작은 성분(13)은 상층에 배열될 수 있다. 이 경우 분리하고자 하는 소정의 성분(11)보다 비중이 작거나 크기가 작은 성분(13)은 제1 회귀방지부(123a)에 의해 방해를 받지 않고 회귀하여 제1 필터(131)를 통과하여 회전챔버(110)로 쉽게 회귀할 수 있게 되는 반면에, 분리하고자 하는 소정의 성분(11)은 제1 회귀방지부(123a)에 의해 방해를 받아 회귀하지 못하게 되어 제1 필터(131)까지 이르지 못함으로써 회전챔버(110)로 회귀할 수 없게 된다. 결과적으로 제1 저장본체(123)에 잔류하게 되는 물질 중에는 분리하고자 하는 소정의 성분(11)의 비율이 높아져 분리효율이 대폭 향상될 수 있게 된다. When the centrifugal force due to the rotation of the rotating chamber 110 becomes smaller or less than a predetermined level, as shown in FIG. 8, the predetermined component to be separated by moving to the first storage body 123 ( 11 may be arranged in the lower layer, and the component 13 having a specific gravity or size smaller than the predetermined component 11 may be arranged in the upper layer. In this case, the component 13 having a specific gravity smaller or smaller than the predetermined component 11 to be separated is returned without being disturbed by the first regression preventing portion 123a and rotated through the first filter 131. While it is possible to easily return to the chamber 110, the predetermined component 11 to be separated is prevented from returning by being disturbed by the first regression preventing portion 123a and thus not reaching the first filter 131. As a result, it is impossible to return to the rotation chamber 110. As a result, in the material remaining in the first storage body 123, the ratio of the predetermined component 11 to be separated is increased, so that the separation efficiency can be greatly improved.
제1 저장덮개(124)는 제1 저장본체(123)의 상부를 커버하는 것이다. 이를 위하여 제1 저장덮개(124)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1에 나타나 있는 바와 같이 제1 저장덮개(124)는 제1 저장본체(123)의 상부에 대응하도록 연장되는 형상으로 이루어질 수 있다. 이러한 제1 저장덮개(124)는 제1 저장본체(123)에 분리할 수 있도록 또는 분리할 수 없도록 결합할 수 있다. The first storage cover 124 covers the upper portion of the first storage body 123. To this end, the first storage cover 124 may be formed in various configurations. For example, as illustrated in FIG. 1, the first storage cover 124 may have a shape extending to correspond to an upper portion of the first storage body 123. The first storage cover 124 may be coupled to the first storage body 123 to be separated or not separated.
제1 저장덮개(124)에는 제1 저장본체(123)에 저장된 분리하고자 하는 소정의 성분(11)을 배출하기 위한 제1 배출구(124a)가 추가될 수 있다. 이 제1 배출구(124a)에 호스와 같은 연결관을 연결하여 저장된 분리하고자 하는 소정의 성분(11)을 용이하게 오염되지 않은 상태에서 외부로 배출시킬 수 있다. 이때 별도로 펌프나 저장탱크가 필요하지 않게 되므로 장치의 구조를 단순화시킬 수 있게 된다.A first outlet 124a may be added to the first storage cover 124 to discharge the predetermined component 11 to be separated stored in the first storage body 123. By connecting a connection pipe such as a hose to the first outlet 124a, the predetermined component 11 to be separated can be easily discharged to the outside in an uncontaminated state. In this case, since a pump or a storage tank are not required separately, the structure of the device can be simplified.
제1 저장덮개(124)는 제1 수평안내부(131)의 상부를 커버하는 부분(115)과 분리할 수 있도록 또는 분리할 수 없도록 결합할 수 있다. The first storage cover 124 may be coupled to or separated from the portion 115 that covers the upper portion of the first horizontal guide 131.
제2 저장부(122)는 제2 수평안내부(114)에 연결되어 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되고 수직안내부(111)를 거쳐 제2 수평안내부(114)에 의해 안내되는 소정의 성분(11)을 저장하는 것이다. 이를 위하여 제2 저장부(122)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1 및 도 2에 나타나 있는 바와 같이 제2 저장부(122)는 제2 저장본체(125)를 구비하며 제2 저장덮개(126)를 더 구비할 수 있다.The second storage part 122 is connected to the second horizontal guide part 114 and separated from the material 10 accommodated inside the rotating chamber 110 by centrifugal force due to the rotation of the rotating chamber 110. The predetermined component 11 guided by the second horizontal guide portion 114 via 111 is stored. To this end, the second storage unit 122 may be configured in various configurations. For example, as illustrated in FIGS. 1 and 2, the second storage unit 122 may include a second storage body 125 and further include a second storage cover 126.
제2 저장본체(125)는 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되고 수직안내부(111)를 거쳐 제2 수평안내부(114)에 의해 안내되는 소정의 성분(11)을 저장하는 것이다. 이를 위하여 제2 저장본체(125)는 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)이 저장될 수 있는 오목한 형상으로 이루어질 수 있다. 예컨대, 예시적으로 도 1 및 도 2에 나타나 있는 바와 같이 제2 저장본체(125)는 그 수직단면이 상측에서 하측으로 갈수록 좁아지는 형상이고 그 수평단면이 원형의 형상으로 이루어짐으로써 전체적으로 역원뿔 형상일 수 있다. 다만, 이에 한정되지 않고 제2 저장본체(125)는 다른 다양한 구성으로 이루어질 수도 있다. 제2 저장본체(125)의 수평단면의 다른 다양한 형상이 도 3 내지 도 6에 예시적으로 나타나 있는 제1 저장본체(123)의 수평단면의 다른 다양한 형상과 유사한 형상으로 이루어질 수 있다. The second storage body 125 is separated from the material 10 contained in the rotating chamber 110 by the centrifugal force caused by the rotation of the rotating chamber 110, and the second horizontal guide portion 114 via the vertical guide portion 111. The predetermined component 11 guided by To this end, the second storage body 125 may have a concave shape in which a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 may be stored. For example, as illustrated in FIGS. 1 and 2, the second storage body 125 has a shape in which the vertical cross section thereof becomes narrower from the upper side to the lower side, and the horizontal cross section has a circular shape, and thus has an inverse conical shape as a whole. Can be. However, the present invention is not limited thereto, and the second storage body 125 may have various other configurations. Other various shapes of the horizontal cross section of the second storage body 125 may be formed in a shape similar to other various shapes of the horizontal cross section of the first storage body 123 illustrated in FIGS. 3 to 6.
제2 저장본체(125)는 적어도 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)이 회전챔버(110)가 정지된 후 저장될 수 있는 정도의 크기를 갖는 것이 바람직하다. The second storage body 125 has a predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by at least centrifugal force due to the rotation of the rotation chamber 110, and the rotation chamber 110 is stopped. It is desirable to have a size that can be stored after being stored.
제2 저장본체(125)는 적어도 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)과 이러한 소정의 성분(11)보다 작은 크기를 갖는 성분(13)이 회전챔버(110)가 정지된 후 함께 저장될 수 있는 정도보다 작은 크기를 갖는 것이 바람직하다. 이에 의하여, 회전챔버(110)의 회전에 따른 원심력이 소정의 수준보다 작게 되는 시점부터 작용하지 않게 되는 시점까지, 소정의 성분(11)보다 작은 크기의 성분(13)이 제2 저장본체(125)로부터 이탈하여 회전챔버(110) 내부로 쉽게 회귀할 수 있게 됨으로써, 분리된 소정의 성분(11)의 분리효율을 대폭 향상시킬 수 있게 된다.The second storage body 125 includes a predetermined component 11 separated from the material 10 contained in the rotating chamber 110 by at least centrifugal force due to the rotation of the rotating chamber 110, and the predetermined component 11. It is preferred that the component 13 having a smaller size is smaller than the extent to which the rotating chamber 110 can be stored together after stopping. As a result, the second storage body 125 has a component 13 having a size smaller than the predetermined component 11 from the time when the centrifugal force due to the rotation of the rotating chamber 110 becomes smaller than the predetermined level to the point at which the rotary chamber 110 does not work. It is possible to easily return to the inside of the rotating chamber 110 by moving away from the), it is possible to significantly improve the separation efficiency of the separated predetermined component (11).
제2 저장본체(125)는 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되어 저장된 소정의 성분(11)이 제2 저장본체(123)에서 회전챔버(110)로 회귀하지 않게 하는 제2 회귀방지부(125a)를 포함하는 것이 바람직하다. 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 물질(10)에서 분리되어 제2 저장본체(125)에 저장된 소정의 성분(11)은, 회전챔버(110)의 회전에 따른 원심력이 작아지거나 작용하지 않게 되면, 원심력보다 중력이 더 크게 작용하게 되어 제2 저장본체(125)에서 이탈하여 회전챔버(110)로 회귀하려고 한다. 이 경우 제2 회귀방지부(125a)에 의하여 제2 저장본체(123)에 저장된 소정의 성분(11)이 회전챔버(110)로 회귀하는 것을 방지할 수 있게 됨으로써 분리효율을 향상시킬 수 있게 된다. 이러한 제2 회귀방지부(125a)는, 예시적으로 도 1에 나타나 있는 바와 같이, 제2 저장본체(125)의 중심과 같은 바닥 부분에서 제2 수평안내부(114)에 연결되는 부분까지 오르막 경사를 이루는 경사면으로 형성될 수 있다. 제2 회귀방지부(125a)를 형성하는 경사면의 경사진 정도나 길이, 형상 등은 제2 저장본체(125)에 저장된 소정의 성분(11)의 종류 및 특성 등에 의하여 적절하게 조절할 수 있다.The second storage body 125 has a predetermined component 11 separated and stored in the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110. It is preferable to include a second regression preventing portion (125a) to prevent the return to the rotating chamber (110). The predetermined component 11 separated from the material 10 contained in the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 and stored in the second storage body 125 is formed of the rotation chamber 110. When the centrifugal force due to the rotation becomes small or does not work, the gravity acts more than the centrifugal force, thereby leaving the second storage body 125 and attempting to return to the rotation chamber 110. In this case, it is possible to prevent the predetermined component 11 stored in the second storage body 123 from returning to the rotation chamber 110 by the second regression preventing unit 125a, thereby improving separation efficiency. . As shown in FIG. 1, the second regression preventing part 125a may ascend from a bottom part such as the center of the second storage body 125 to a part connected to the second horizontal guide part 114. It may be formed as an inclined surface forming an inclination. The inclination degree, length, shape, etc. of the inclined surface forming the second regression preventing part 125a can be appropriately adjusted according to the kind and characteristics of the predetermined component 11 stored in the second storage body 125.
제2 회귀방지부(125a)는 제2 저장본체(125)에 저장된 소정의 성분(11)의 회전챔버(110)로의 회귀를 방지하는 효과를 높이는 것이 바람직하나, 이 경우 제2 저장본체(125)에 소정의 성분(11)과 함께 저장된 그보다 작은 성분(13)의 회전챔버(110)로의 회귀를 억제하는 효과도 함께 높아져서 바람직하지 않게 된다. 따라서 제2 회귀방지부(125a)는 제2 저장본체(125)에 저장된 소정의 성분(11)의 회전챔버(110)로의 회귀를 방지하는 효과는 높이면서 제2 저장본체(125)에 소정의 성분(11)과 함께 저장된 그보다 작은 성분(13)의 회전챔버(110)로의 회귀를 억제하는 효과는 낮추도록 그 크기나 형상 등을 적절하게 조절하는 것이 바람직하다.Preferably, the second regression preventing part 125a enhances the effect of preventing the regression of the predetermined component 11 stored in the second storage body 125 into the rotation chamber 110. In this case, the second storage body 125 The effect of suppressing the regression of the smaller component 13 into the rotating chamber 110 stored together with the predetermined component 11 is also high, which is undesirable. Accordingly, the second regression preventing part 125a has a predetermined effect on the second storage body 125 while increasing the effect of preventing the return of the predetermined component 11 stored in the second storage body 125 to the rotation chamber 110. It is desirable to appropriately adjust the size, shape, and the like so that the effect of suppressing the return of the smaller component 13 stored with the component 11 into the rotation chamber 110 is lowered.
회전챔버(110)의 회전에 따른 원심력이 작용하게 되면, 예시적으로 도 7에 나타나 있는 바와 같이, 회전챔버(110)에 수용된 물질(10) 중에서 제2 필터(132)를 통과하지 못한 성분(12)은 제2 저장본체(125)로 이동하지 못하여 회전챔버(110)에 남게 되고, 분리하고자 하는 소정의 성분(11)은 제2 필터(132)를 통과하여 제2 저장본체(125)로 이동하게 된다. 그러나 이 경우 분리대상은 아니지만 분리하고자 하는 소정의 성분(11)보다 비중 또는 크기가 더 작은 성분(13)도 제2 필터(132)를 통과하여 제2 저장본체(125)로 이동하게 된다. When the centrifugal force due to the rotation of the rotating chamber 110 is applied, as shown in FIG. 7, the component 10 that has not passed through the second filter 132 of the material 10 contained in the rotating chamber 110 ( 12 does not move to the second storage body 125 and remains in the rotating chamber 110, and the predetermined component 11 to be separated passes through the second filter 132 to the second storage body 125. Will move. However, in this case, the component 13 which is not a separation target but smaller in specific gravity or size than the predetermined component 11 to be separated also moves through the second filter 132 to the second storage body 125.
회전챔버(110)의 회전에 따른 원심력이 소정의 수준보다 작아지거나 작용하지 않게 되면, 예시적으로 도 8에 나타나 있는 바와 같이, 제2 저장본체(125)로 이동한 분리하고자 하는 소정의 성분(11)은 하층에 배열되고 소정의 성분(11)보다 비중이나 크기가 더 작은 성분(13)은 상층에 배열될 수 있다. 이 경우 분리하고자 하는 소정의 성분(11)보다 비중이 작거나 크기가 작은 성분(13)은 제2 회귀방지부(125a)에 의해 방해를 받지 않고 회귀하여 제2 필터(132)를 통과하여 회전챔버(110)로 쉽게 회귀할 수 있게 되는 반면에, 분리하고자 하는 소정의 성분(11)은 제2 회귀방지부(125a)에 의해 방해를 받아 회귀하지 못하게 되어 제2 필터(131)까지 이르지 못함으로써 회전챔버(110)로 회귀할 수 없게 된다. 결과적으로 제2 저장본체(123)에 잔류하게 되는 물질 중에는 분리하고자 하는 소정의 성분(11)의 비율이 높아져 분리효율이 대폭 향상될 수 있게 된다.When the centrifugal force due to the rotation of the rotating chamber 110 is smaller or less than a predetermined level, as shown in FIG. 8, as shown in FIG. 8, a predetermined component to be separated by moving to the second storage body 125 ( 11 may be arranged in the lower layer, and the component 13 having a specific gravity or size smaller than the predetermined component 11 may be arranged in the upper layer. In this case, the component 13 having a specific gravity smaller or smaller than the predetermined component 11 to be separated is returned without being disturbed by the second regression preventing portion 125a to rotate through the second filter 132. While it is possible to easily return to the chamber 110, the predetermined component 11 to be separated is prevented from returning by being disturbed by the second regression preventing portion 125a and thus not reaching the second filter 131. As a result, it is impossible to return to the rotation chamber 110. As a result, in the material remaining in the second storage body 123, the ratio of the predetermined component 11 to be separated is increased, so that the separation efficiency can be greatly improved.
제2 저장덮개(126)는 제2 저장본체(125)의 상부를 커버하는 것이다. 이를 위하여 제2 저장덮개(126)는 다양한 구성으로 이루어질 수 있다. 예컨대, 예시적으로 도 1에 나타나 있는 바와 같이 제2 저장덮개(126)는 제2 저장본체(125)의 상부에 대응하도록 연장되는 형상으로 이루어질 수 있다. 이러한 제2 저장덮개(126)는 제2 저장본체(125)에 분리할 수 있도록 또는 분리할 수 없도록 결합할 수 있다. The second storage cover 126 covers the upper portion of the second storage body 125. To this end, the second storage cover 126 may be formed in various configurations. For example, as illustrated in FIG. 1, the second storage cover 126 may have a shape extending to correspond to an upper portion of the second storage body 125. The second storage cover 126 may be coupled to the second storage body 125 to be separated or not separated.
제2 저장덮개(126)에는 제2 저장본체(125)에 저장된 분리하고자 하는 소정의 성분(11)을 배출하기 위한 제2 배출구(126a)가 추가될 수 있다. 이 제2 배출구(126a)에 호스와 같은 연결관을 연결하여 저장된 소정의 성분(11)을 용이하게 오염되지 않은 상태에서 외부로 배출시킬 수 있다. 이때 별도로 펌프나 저장탱크가 필요하지 않게 되므로 장치의 구조를 단순화시킬 수 있게 된다.A second outlet 126a may be added to the second storage cover 126 to discharge the predetermined component 11 to be separated stored in the second storage body 125. A connection pipe such as a hose may be connected to the second outlet 126a to easily discharge the predetermined component 11 stored therein without being contaminated. In this case, since a pump or a storage tank are not required separately, the structure of the device can be simplified.
제2 저장덮개(126)는 제2 수평안내부(132)의 상부를 커버하는 부분(115) 과 분리할 수 있도록 또는 분리할 수 없도록 결합할 수 있다. The second storage cover 126 may be combined to be separated from or separated from the portion 115 covering the upper portion of the second horizontal guide 132.
필터(131)(132)는 회전챔버(110)의 회전에 따른 원심력에 의해 회전챔버(110) 내부에 수용된 물질(10)에서 분리된 소정의 성분(11)이 통과되는 것이다. 이를 위하여 필터(131)(132)는 다양한 구성으로 이루어질 수 있다. 예컨대, 필터(131)(132)는 제1 필터(131)와 제2 필터(132)를 구비할 수 있다. 다만, 이에 한정되지 않고 필터(131)(132)는 하나의 필터 또는 세 개 이상의 필터로 이루어질 수도 있다.The filters 131 and 132 pass through a predetermined component 11 separated from the material 10 contained in the rotary chamber 110 by centrifugal force due to the rotation of the rotary chamber 110. To this end, the filters 131 and 132 may be formed in various configurations. For example, the filters 131 and 132 may include a first filter 131 and a second filter 132. However, the present invention is not limited thereto, and the filters 131 and 132 may be formed of one filter or three or more filters.
제1 필터(131)는 회전챔버(110)와 제1 저장부(121) 사이에 배열될 수 있다. 예컨대, 제1 필터(131)는, 예시적으로 도 7에 나타나 있는 바와 같이, 회전챔버(110)의 수직안내부(111)와 제1 저장부(121) 사이의 제1 수평안내부(113) 상에 배열될 수 있다. 이에 의하여 제1 필터(131)는 회전챔버(110)의 회전에 따른 원심력에 의해 회전챔버(110) 내부에 수용된 물질(10)이 제1 저장부(121) 쪽으로 전부 이동하지 않고 분리하고자 하는 소정의 성분(11)보다 비중 또는 크기가 큰 성분(12)을 걸러낼 수 있게 된다. 다만, 이에 한정되지 않고 제1 필터(131)는 회전챔버(110)와 제1 저장부(121) 사이에서 임의의 위치에 배열될 수 있다. The first filter 131 may be arranged between the rotation chamber 110 and the first storage 121. For example, as illustrated in FIG. 7, the first filter 131 may include a first horizontal guide 113 between the vertical guide 111 and the first storage 121 of the rotating chamber 110. ) Can be arranged on. As a result, the first filter 131 is a predetermined material which is to be separated without moving all of the material 10 contained in the rotating chamber 110 toward the first storage part 121 by the centrifugal force due to the rotation of the rotating chamber 110. The component 12 having a specific gravity or size larger than that of the component 11 can be filtered out. However, the present invention is not limited thereto, and the first filter 131 may be arranged at an arbitrary position between the rotation chamber 110 and the first storage 121.
제1 필터(131)는 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11) 또는 그보다 더 작은 크기나 비중의 성분(13)은 통과시키고 소정의 성분(11)보다 더 큰 크기나 비중의 성분(12)은 통과시키지 않는다. 예컨대, 제1 필터(131)는 회전챔버(110) 내부에 수용되어 원심분리 대상이 되는 물질(10)에 포함된 혈구(blood corpuscle), 혈장, 유리지방(free oil) 및 줄기세포 기타 불순물과 같이 분리하고자 하는 소정의 성분(11) 및 그보다 작은 크기 또는 비중의 성분(13)은 통과시키지만, 구형에 가까운 고체 등과 같이 상대적으로 큰 크기나 비중의 성분(12)은 통과시키지 않는다. The first filter 131 passes a predetermined component 11 or a smaller size or specific gravity component 13 to be separated from the material 10 contained in the rotary chamber 110 and passes the predetermined component 11. Component 12 of greater size or specific gravity is not passed through. For example, the first filter 131 is accommodated in the rotating chamber 110 and contained in blood 10 (blood corpuscle), plasma, free oil and stem cells and other impurities contained in the material 10 to be centrifuged. Likewise, the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity are passed, but the component 12 having a relatively larger size or specific gravity, such as a solid close to a sphere, is not passed.
제1 필터(131)는 메쉬(mesh)의 크기가 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)에 따라 소정의 범위로 조절할 수 있다. 예컨대, 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)이 줄기세포일 경우에는 일반적으로 제1 필터(131)의 메쉬 크기를 1μm 내지 2000μm로 조절할 수 있다. 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)이 줄기세포일 경우 10G 이상의 원심력이 작용할 때에는 제1 필터(131)의 메쉬 크기를 10μm 내지 1000μm로 조절하는 것이 바람직하다. 이와 같이 제1 필터(131)의 메쉬 크기를 조절함으로써 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)보다 더 큰 크기나 비중의 성분(12)을 걸러낼 수 있게 된다.The first filter 131 may adjust the size of the mesh in a predetermined range according to the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110. For example, when the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 is a stem cell, the mesh size of the first filter 131 may generally be adjusted to 1 μm to 2000 μm. When the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 is a stem cell, when the centrifugal force of 10G or more is applied, the mesh size of the first filter 131 may be adjusted to 10 μm to 1000 μm. desirable. As such, by adjusting the mesh size of the first filter 131, the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 may be filtered out. It becomes possible.
제1 필터(131)는 그 횡단면의 면적을 원심분리속도나 원심분리수율 등에 따라 적절하게 조절할 수 있다. 예컨대, 원심분리속도를 높이기 위해 제1 필터(131)의 횡단면의 면적을 크게 할 수도 있고 원심분리수율을 높이기 위해 제1 필터(131)의 횡단면의 면적을 작게 할 수도 있다. 다만, 이에 한정되지 않고 제1 필터(131)의 횡단면의 면적은 다양하게 조절될 수 있다.The first filter 131 can appropriately adjust the area of the cross section according to the centrifugation speed, the centrifugation yield, or the like. For example, the area of the cross section of the first filter 131 may be increased to increase the centrifugation speed, or the area of the cross section of the first filter 131 may be reduced to increase the centrifugation yield. However, the present invention is not limited thereto, and the area of the cross section of the first filter 131 may be variously adjusted.
제2 필터(132)는 회전챔버(110)와 제2 저장부(122) 사이에 배열될 수 있다. 예컨대, 제2 필터(132)는, 예시적으로 도 7에 나타나 있는 바와 같이, 회전챔버(110)의 수직안내부(111)와 제2 저장부(122) 사이의 제2 수평안내부(114) 상에 배열될 수 있다. 이에 의하여 제2 필터(132)는 회전챔버(110)의 회전에 따른 원심력에 의해 회전챔버(110) 내부에 수용된 물질(10)이 제2 저장부(122) 쪽으로 전부 이동하지 않고 분리하고자 하는 소정의 성분(11)보다 비중 또는 크기가 큰 성분(12)을 걸러낼 수 있게 된다. 다만, 이에 한정되지 않고 제2 필터(132)는 회전챔버(110)와 제2 저장부(122) 사이에서 임의의 위치에 배열될 수 있다. The second filter 132 may be arranged between the rotation chamber 110 and the second storage unit 122. For example, as illustrated in FIG. 7, the second filter 132 may include a second horizontal guide portion 114 between the vertical guide portion 111 and the second storage portion 122 of the rotating chamber 110. ) Can be arranged on. As a result, the second filter 132 may be separated from the material 10 contained in the rotating chamber 110 by the centrifugal force due to the rotation of the rotating chamber 110 without moving to the second storage part 122. The component 12 having a specific gravity or size larger than that of the component 11 can be filtered out. However, the present invention is not limited thereto, and the second filter 132 may be arranged at an arbitrary position between the rotation chamber 110 and the second storage unit 122.
제2 필터(132)는 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11) 또는 그보다 더 작은 크기나 비중의 성분(13)은 통과시키고 소정의 성분(11)보다 더 큰 크기나 비중의 성분(12)은 통과시키지 않는다. 예컨대, 제2 필터(132)는 회전챔버(110) 내부에 수용되어 원심분리 대상이 되는 물질(10)에 포함된 혈구(blood corpuscle), 혈장, 유리지방(free oil) 및 줄기세포 기타 불순물과 같이 분리하고자 하는 소정의 성분(11) 및 그보다 작은 크기 또는 비중의 성분(13)은 통과시키지만, 구형에 가까운 고체 등과 같이 상대적으로 큰 크기나 비중의 성분(12)은 통과시키지 않는다. The second filter 132 passes through a predetermined component 11 or a component 13 having a smaller size or specific gravity to be separated from the material 10 contained in the rotary chamber 110 and the predetermined component 11. Component 12 of greater size or specific gravity is not passed through. For example, the second filter 132 is accommodated in the rotating chamber 110 and contained in blood 10 (blood corpuscle), plasma, free oil and stem cells and other impurities contained in the material 10 to be centrifuged. Likewise, the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity are passed, but the component 12 having a relatively larger size or specific gravity, such as a solid close to a sphere, is not passed.
제2 필터(132)는 메쉬(mesh)의 크기가 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)에 따라 소정의 범위로 조절할 수 있다. 예컨대, 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)이 줄기세포일 경우에는 일반적으로 제2 필터(132)의 메쉬 크기를 1μm 내지 2000μm로 조절할 수 있다. 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)이 줄기세포일 경우 10G 이상의 원심력이 작용할 때에는 제2 필터(132)의 메쉬 크기를 10μm 내지 1000μm로 조절하는 것이 바람직하다. 이와 같이 제2 필터(132)의 메쉬 크기를 조절함으로써 회전챔버(110) 내부에 수용된 물질(10)에서 분리하고자 하는 소정의 성분(11)보다 더 큰 크기나 비중의 성분(12)을 걸러낼 수 있게 된다.The second filter 132 may adjust the size of the mesh in a predetermined range according to the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110. For example, when the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 is a stem cell, the mesh size of the second filter 132 may generally be adjusted to 1 μm to 2000 μm. When the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 is a stem cell, when a centrifugal force of 10G or more is applied, it is preferable to adjust the mesh size of the second filter 132 to 10 μm to 1000 μm. desirable. As such, by adjusting the mesh size of the second filter 132, the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated from the material 10 contained in the rotating chamber 110 may be filtered out. It becomes possible.
제2 필터(132)는 그 횡단면의 면적을 원심분리속도나 원심분리수율 등에 따라 적절하게 조절할 수 있다. 예컨대, 원심분리속도를 높이기 위해 제2 필터(132)의 횡단면의 면적을 크게 할 수도 있고 원심분리수율을 높이기 위해 제2 필터(132)의 횡단면의 면적을 작게 할 수도 있다. 다만, 이에 한정되지 않고 제2 필터(132)의 횡단면의 면적은 다양하게 조절될 수 있다.The second filter 132 can appropriately adjust the area of the cross section according to the centrifugation speed, centrifugation yield, or the like. For example, the area of the cross section of the second filter 132 may be increased to increase the centrifugation speed, or the area of the cross section of the second filter 132 may be reduced to increase the centrifugation yield. However, the present invention is not limited thereto, and the area of the cross section of the second filter 132 may be variously adjusted.
본 실시례의 원심분리기의 작용에 대하여 설명한다.The operation of the centrifuge of the present embodiment will be described.
회전챔버(110)의 덮개(112)의 주입구(112a)를 통해 원심분리의 대상이 되는 물질(10)이 회전챔버(110)의 내부로 주입되면, 회전챔버(110)가 회전되지 전에는, 예시적으로 도 1에 나타나 있는 바와 같이, 원심분리의 대상이 되는 물질(10)은 회전챔버(110)의 중심 하측에 배열된다.When the material 10 to be centrifuged through the injection hole 112a of the cover 112 of the rotary chamber 110 is injected into the rotary chamber 110, the rotary chamber 110 is not rotated before 1, the material 10 to be centrifuged is arranged below the center of the rotation chamber 110.
회전챔버(110)가 회전하기 시작하면, 회전챔버(110)의 회전에 따른 원심력에 의하여 회전챔버(110) 내부에 수용된 원심분리의 대상이 되는 물질(10)은 회전챔버(110)의 수직안내부(111)의 내측 표면을 따라 하측에서 상측으로 이동하기 시작한다.When the rotation chamber 110 starts to rotate, the material 10 to be subjected to the centrifugal separation accommodated inside the rotation chamber 110 by the centrifugal force due to the rotation of the rotation chamber 110 is vertically guided by the rotation chamber 110. It starts to move from bottom to top along the inner surface of the part 111.
회전챔버(110)의 회전에 따른 원심력이 증가하여 소정의 수준에 도달하게 되면, 회전챔버(110) 내부에 수용된 원심분리의 대상이 되는 물질(10)은, 예시적으로 도 7에 나타나 있는 바와 같이, 회전챔버(110)의 수직안내부(111)와 제1 및 제2 수평안내부(113)(114)에 의해 안내되어 제1 및 제2 필터(131)(132)를 통과하여 제1 및 제2 저장부(121)(122)에 저장된다. 이때 회전챔버(110) 내부에 수용된 원심분리의 대상이 되는 물질(10) 중에서 분리하고자 하는 소정의 성분(11)과 그보다 더 작은 크기나 비중의 성분(13)은 제1 및 제2 필터(131)(132)를 통과하여 제1 및 제2 저장부(121)(122)로 이동되지만, 분리하고자 하는 소정의 성분(11)보다 더 큰 크기나 비중의 성분(12)은 제1 및 제2 필터(131)(132)를 통과하지 못하여 제1 및 제2 저장부(121)(122)로 이동되지 못한다. 이 경우 분리하고자 하는 소정의 성분(11)과 그보다 더 작은 크기나 비중의 성분(13)은 제1 및 제2 저장부(121)(122)의 저장용량보다 큰 함량일 때에는 그 성분(13)의 일부만이 제1 및 제2 저장부(121)(122)로 이동하게 된다. 이때 분리하고자 하는 소정의 성분(11)은 제1 및 제2 저장부(121)(122)의 하층에 배열되고 분리하고자 하는 소정의 성분(11)보다 작은 크기나 비붕의 성분(13)은 제1 및 제2 저장부(121)(122)의 상층에 배열된다.When the centrifugal force due to the rotation of the rotating chamber 110 increases to reach a predetermined level, the material 10 to be subjected to the centrifugal separation accommodated inside the rotating chamber 110 is exemplarily shown in FIG. 7. Likewise, the first and second horizontal guides 113 and 114 of the rotary chamber 110 are guided by the first and second horizontal guides 113 and 114 to pass through the first and second filters 131 and 132. And second storage units 121 and 122. At this time, the predetermined component 11 to be separated and the smaller component 13 having a smaller size or specific gravity from the material 10 to be subjected to centrifugal separation accommodated inside the rotating chamber 110 may include the first and second filters 131. The first and second reservoirs 121 and 122 are moved through the 132, but the component 12 having a larger size or specific gravity than the predetermined component 11 to be separated may be the first and second. It cannot pass through the filters 131 and 132 and thus cannot be moved to the first and second storage units 121 and 122. In this case, the predetermined component 11 to be separated and the component 13 having a smaller size or specific gravity than that of the component 13 are larger than the storage capacity of the first and second storage units 121 and 122. Only a part of the first and second storage units 121 and 122 move. At this time, the predetermined component 11 to be separated is arranged in a lower layer of the first and second storage parts 121 and 122, and the component 13 having a smaller size or a non-bore than the predetermined component 11 to be separated is formed. It is arranged on the upper layer of the first and second storage (121) 122.
회전챔버(110)의 회전에 따른 원심력을 소정의 수준보다 작은 수준으로 감소시키게 되면, 제1 및 제2 저장부(121)(122)로 이동된 분리하고자 하는 소정의 성분(11)과 그보다 더 작은 크기나 비중의 성분(13)은 중력의 영향이 더 커지게 된다. 따라서 예시적으로 도 8에 나타나 있는 바와 같이, 제1 및 제2 저장부(121)(122)의 상층에 배열된 분리하고자 하는 소정의 성분(11)보다 더 작은 크기나 비중의 성분(13)은 제1 및 제2 저장부(121)(122)에서 회전챔버(110)로 회귀하게 된다. 이 경우 분리하고자 하는 소정의 성분(11)보다 더 작은 크기나 비중의 성분(13)은 제1 및 제2 저장부(121)(122)의 상층에 배열되어 있기 때문에 제1 및 제2 회귀방지부(123a)(125a)에 의하여 방해를 받지 않고 원활하게 회전챔버(110)로 회귀하게 된다. 그러나, 제1 및 제2 저장부(121)(122)의 하층에 배열된 분리하고자 하는 소정의 성분(11)은 원심력보다 중력의 영향을 더 크게 받게 되더라도 제1 및 제2 회귀방지부(123a)(125a)에 의하여 회전챔버(110)로 회귀하는 것이 방지되고 제1 및 제2 저장부(121)(122)에 그대로 잔류하게 된다.When the centrifugal force due to the rotation of the rotating chamber 110 is reduced to a level smaller than the predetermined level, the predetermined component 11 to be separated and moved to the first and second storage portions 121 and 122 and the like are more than that. The smaller size or specific gravity component 13 will have a greater gravity effect. Accordingly, as illustrated in FIG. 8, the component 13 having a smaller size or specific gravity than the predetermined component 11 to be separated is arranged on the upper layers of the first and second storage portions 121 and 122. Is returned to the rotation chamber 110 in the first and second storage (121, 122). In this case, since the components 13 having a smaller size or specific gravity than the predetermined components 11 to be separated are arranged on the upper layers of the first and second storage parts 121 and 122, the first and second regression prevention operations are performed. It is smoothly returned to the rotation chamber 110 without being disturbed by the parts 123a and 125a. However, the predetermined components 11 to be separated arranged in the lower layers of the first and second storage parts 121 and 122 may have a greater influence of gravity than the centrifugal force, but the first and second regression preventing parts 123a. Return to the rotating chamber 110 is prevented by the 125a and remains in the first and second storage parts 121 and 122 as they are.
회전챔버(110)의 회전에 따른 원심력이 작용하지 않게 되면, 제1 및 제2 저장부(121)(122)에는, 예시적으로 도 9에 나타나 있는 바와 같이, 분리하고자 하는 소정의 성분(11)은 남고 그 소정의 성분(11)보다 작은 크기나 비중의 성분(13)은 남지 않게 된다. 이후 제1 및 제2 저장부(121)(122)에 남은 분리하고자 하는 소정의 성분(11)은 제1 및 제2 배출구(124a)(126a)를 통해 외부로 배출하게 된다. 또는 제1 및 제2 저장부(121)(122)에 남은 분리하고자 하는 소정의 성분(11)을 외부로 배출하기 전에, 회전챔버(110)에 원심분리의 대상이 되는 물질(10)을 추가로 주입하여 원심분리과정을 반복함으로써, 제1 및 제2 저장부(121)(122)에 남은 분리하고자 하는 소정의 성분(11)의 함량을 높일 수도 있다.When the centrifugal force due to the rotation of the rotating chamber 110 is not applied, the first and second storage parts 121 and 122, as shown in FIG. ) Remains, and no component 13 of a size or specific gravity smaller than the predetermined component 11 remains. Thereafter, the predetermined components 11 to be separated in the first and second storage parts 121 and 122 are discharged to the outside through the first and second discharge ports 124a and 126a. Alternatively, before discharging the predetermined component 11 to be separated remaining in the first and second storage parts 121 and 122 to the outside, the substance 10 to be centrifuged is added to the rotating chamber 110. By repeating the centrifugation process by injecting into, the content of the predetermined component 11 to be separated in the first and second storage parts 121 and 122 may be increased.
제1 및 제2 저장부(121)(122)에 남은 분리하고자 하는 소정의 성분(11)을 외부로 배출한 후에는 위 과정을 반복하게 된다.After the predetermined components 11 to be separated in the first and second storage parts 121 and 122 are discharged to the outside, the above process is repeated.
(실시례 2)(Example 2)
본 실시례는, 예시적으로 도 10에 나타나 있는 바와 같이, 원심분리방법(200)으로서, 원심분리의 대상이 되는 물질에 원심력이 작용하도록 원심분리의 대상이 되는 물질을 수용하는 회전챔버를 회전시키는 단계(210)를 포함한다. 원심분리의 대상이 되는 물질을 회전챔버에 주입할 때에는, 회전챔버의 주입구에 구비된 덮개를 개방하고 물질을 주입하거나, 주입구에 연결된 주입관을 이용하여 물질을 주입할 수 있다. 10, the centrifugal separation method 200, as shown in FIG. 10, rotates a rotating chamber containing a material to be centrifuged so that centrifugal force acts on the material to be centrifuged. Step 210. When injecting the material to be centrifuged into the rotating chamber, the cover provided in the injection port of the rotary chamber can be opened and the material can be injected, or the material can be injected using an injection tube connected to the injection port.
이후, 회전챔버의 회전에 따른 원심력을 증가시켜 회전챔버에 수용된 물질을 필터에 통과시킴으로써 회전챔버에 수용된 물질에서 소정의 성분과 그보다 작은 크기의 성분을 분리하여 저장부에 저장하는 단계(220)를 진행한다. Thereafter, by increasing the centrifugal force according to the rotation of the rotating chamber and passing the material contained in the rotating chamber through the filter, a step of separating 220 a predetermined component and a smaller size component from the material contained in the rotating chamber and storing it in the storage unit 220. Proceed.
회전챔버의 회전에 따른 원심력이 작용하면, 회전챔버에 수용된 물질은, 회전챔버의 경사진 내측 표면을 따라 하측에서 상측으로 이동하여 상측 외부 쪽에 마련된 필터를 통과하여 저장부에 도달하게 된다. 이때 소정의 성분보다 큰 크기의 성분은 필터를 통과하지 못하게 된다. 저장부의 상층에는 소정의 성분이 배열되고 저장부의 하층에는 소정의 성분보다 작은 크기의 성분이 배열된다.When centrifugal force acts upon the rotation of the rotating chamber, the material contained in the rotating chamber moves from the lower side to the upper side along the inclined inner surface of the rotating chamber and passes through the filter provided at the upper outer side to reach the storage unit. At this time, a component having a size larger than the predetermined component does not pass through the filter. Predetermined components are arranged in the upper layer of the storage portion, and smaller components are arranged in the lower layer of the storage portion.
그 다음, 회전챔버의 회전에 따른 원심력을 감소시켜 저장부에 저장된 소정의 성분은 잔존시키고 저장부에 저장된 소정의 성분보다 작은 크기의 성분은 중력에 의하여 회전챔버로 회귀시키는 단계(230)를 진행한다. 여기서, 저장부에 저장된 소정의 성분은 중력에 의하여 회전챔버로 회귀하는 것이 차단된다. 이를 위하여 저장부에 소정의 성분이 중력에 의하여 회전챔버로 회귀하는 경로를 차단하는 회귀방지부가 형성될 수 있다. 결과적으로 저장부에는 소정의 성분은 잔존하고 소정의 성분보다 작은 크기의 성분은 잔존하지 않게 된다.Then, the centrifugal force according to the rotation of the rotating chamber is reduced so that a predetermined component stored in the storage unit remains and a component having a smaller size than the predetermined component stored in the storage unit returns to the rotating chamber by gravity (230). do. Here, the predetermined component stored in the storage unit is blocked from returning to the rotation chamber by gravity. To this end, a regression prevention unit may be formed in the storage unit to block a path in which a predetermined component returns to the rotation chamber by gravity. As a result, predetermined components remain in the storage unit, and components smaller in size than the predetermined components remain.
이후, 저장부에 잔존하는 소정의 성분을 외부로 배출하는 단계(240)를 진행한다. 이러한 배출은 저장부에 형성된 배출구에 연결된 호스를 통해 이루어질 수 있다. Thereafter, step 240 of discharging the predetermined component remaining in the storage unit to the outside. This discharge may be through a hose connected to an outlet formed in the reservoir.
본 발명은 대용량의 물질을 분리할 수 있는 원심분리기 및 원심분리방법에 이용할 수 있다.The present invention can be used in a centrifuge and a centrifugal separation method capable of separating a large amount of material.

Claims (13)

  1. 내부에 수용된 물질에 원심력이 작용하도록 회전할 수 있는 회전챔버;A rotating chamber rotatable so that centrifugal force acts on the material contained therein;
    회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분이 저장되는 저장부; 및A storage unit for storing a predetermined component separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber; And
    저장부와 회전챔버 사이에 배열되며, 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분이 저장부에 저장되기 위해 통과하는 필터를 구비하고,Arranged between the storage unit and the rotating chamber, and provided with a filter for passing a predetermined component separated from the material contained in the rotating chamber by the centrifugal force in accordance with the rotation of the rotating chamber to be stored in the storage unit,
    저장부는 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리되어 저장부에 저장된 소정의 성분이 저장부에서 회전챔버로 회귀하지 않게 하는 회귀방지부를 포함하는 원심분리기.The storage unit is a centrifugal separator including a regression prevention unit that is separated from the material contained in the rotating chamber by the centrifugal force according to the rotation of the rotating chamber to prevent a predetermined component stored in the storage unit from returning to the rotating chamber from the storage unit.
  2. 청구항 1에 있어서,The method according to claim 1,
    회전챔버의 수직단면은 상측에서 하측으로 갈수록 좁아지고 회전챔버의 수평단면의 면적은 회전챔버의 상측에서 하측으로 갈수록 작아지는 원심분리기.The vertical section of the rotating chamber becomes narrower from the upper side to the lower side, and the area of the horizontal section of the rotating chamber becomes smaller from the upper side to the lower side of the rotating chamber.
  3. 청구항 1에 있어서,The method according to claim 1,
    저장부는 회전챔버의 회전중심축을 기준으로 대칭이 되는 위치에 배열되는 복수의 저장요소들을 포함하는 원심분리기.The storage unit comprises a plurality of storage elements arranged in a position symmetrical with respect to the center of rotation axis of the rotating chamber.
  4. 청구항 1에 있어서,The method according to claim 1,
    회귀방지부는 회전챔버의 회전에 따른 원심력에 의해 회전챔버 내부에 수용된 물질에서 분리되어 저장부에 저장된 소정의 성분보다 작은 크기의 성분은 저장부에서 회전챔버로 회귀하게 하는 원심분리기.The regression preventing part is separated from the material contained in the rotating chamber by the centrifugal force due to the rotation of the rotating chamber, and the component having a size smaller than a predetermined component stored in the storage unit returns to the rotating chamber from the storage unit.
  5. 청구항 1에 있어서,The method according to claim 1,
    회귀방지부는 저장부의 바닥에서 회전챔버에 연결되는 상부까지 오르막 경사를 이루는 경사면으로 형성되어 있는 원심분리기.The anti-regression unit is a centrifuge formed with an inclined surface that forms an uphill slope from the bottom of the reservoir to an upper portion connected to the rotating chamber.
  6. 청구항 1에 있어서,The method according to claim 1,
    필터는 회전챔버 내부에 수용된 물질에서 분리된 소정의 성분보다 큰 크기의 성분은 통과시키지 않는 원심분리기.The filter is a centrifuge that does not pass a component of a size larger than a predetermined component separated from the material contained in the rotating chamber.
  7. 청구항 1에 있어서,The method according to claim 1,
    필터는 메쉬의 크기가 1μm 내지 2000μm로 조절되어 있는 원심분리기.The filter is a centrifuge having a mesh size of 1μm to 2000μm.
  8. 청구항 1에 있어서,The method according to claim 1,
    필터는 메쉬의 크기가 10μm 내지 1000μm로 조절되어 있는 원심분리기.The filter is a centrifuge having a mesh size of 10μm to 1000μm.
  9. 청구항 1에 있어서,The method according to claim 1,
    회전챔버의 상부를 커버하는 덮개와 저장부의 상부를 커버하는 저장덮개를 더 구비하는 원심분리기.A centrifuge further comprising a cover covering the top of the rotating chamber and a storage cover covering the top of the reservoir.
  10. (1) 원심분리의 대상이 되는 물질에 원심력이 작용하도록 원심분리의 대상이 되는 물질을 수용하는 회전챔버를 회전시키는 단계;(1) rotating a rotating chamber containing the material to be centrifuged so that centrifugal force acts on the material to be centrifuged;
    (2) 회전챔버의 회전에 따른 원심력을 증가시켜 회전챔버에 수용된 물질을 필터에 통과시킴으로써 회전챔버에 수용된 물질에서 소정의 성분과 그보다 작은 크기의 성분을 분리하여 저장부에 저장하는 단계;(2) increasing the centrifugal force according to the rotation of the rotating chamber to pass the material contained in the rotating chamber to the filter to separate a predetermined component and a smaller sized component from the material contained in the rotating chamber and to store it in the storage unit;
    (3) 회전챔버의 회전에 따른 원심력을 감소시켜 저장부에 저장된 소정의 성분은 잔존시키고 저장부에 저장된 소정의 성분보다 작은 크기의 성분은 중력에 의하여 회전챔버로 회귀시키는 단계; 및(3) reducing the centrifugal force due to the rotation of the rotating chamber so that a predetermined component stored in the storage unit remains and a component having a size smaller than the predetermined component stored in the storage unit returns to the rotating chamber by gravity; And
    (4) 저장부에 잔존하는 소정의 성분을 외부로 배출하는 단계를 포함하는 원심분리방법.(4) a centrifugation method comprising the step of discharging the predetermined component remaining in the storage to the outside.
  11. 청구항 10에 있어서,The method according to claim 10,
    (2) 단계의 필터는 회전챔버에 수용된 물질 중에서 분리하고자 하는 소정의 성분보다 큰 크기의 성분을 통과시키지 않는 원심분리방법.The centrifugal separation method of step (2) does not pass a component having a size larger than a predetermined component to be separated from the material contained in the rotating chamber.
  12. 청구항 10에 있어서,The method according to claim 10,
    (3) 단계의 저장부에 저장된 소정의 성분은 중력에 의하여 회전챔버로 회귀하는 것을 차단하는 원심분리방법.(3) the centrifugal separation method for preventing the predetermined component stored in the storage unit from returning to the rotating chamber by gravity.
  13. 청구항 10에 있어서,The method according to claim 10,
    (4) 단계의 저장부에 잔존하는 소정의 성분은 저장부에 형성된 배출구에 연결된 배출관을 통해 외부로 배출되는 원심분리방법.The predetermined component remaining in the storage unit of step (4) is discharged to the outside through the discharge pipe connected to the discharge port formed in the storage unit.
PCT/KR2016/014362 2015-12-09 2016-12-08 Centrifuge and centrifugation method WO2017099487A1 (en)

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CN201680071465.3A CN108367302B (en) 2015-12-09 2016-12-08 Centrifugal separator and centrifugal separation method
US15/781,548 US11103883B2 (en) 2015-12-09 2016-12-08 Centrifuge and centrifugation method using filter

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019033691A (en) * 2017-08-16 2019-03-07 ヒヨン イ Method and device for separating interstitial cell from biological tissue without use of enzyme

Citations (5)

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Publication number Priority date Publication date Assignee Title
JPH11314011A (en) * 1998-05-06 1999-11-16 Toshimasa Yamamoto Separation member and separation method
KR100680136B1 (en) * 2006-06-30 2007-02-07 메디칸(주) Centrifuge
KR100721258B1 (en) * 2005-12-15 2007-05-23 유명기 Impurities filtration device using centrifugal force and filter
KR20110083671A (en) * 2008-11-14 2011-07-20 만 운트 훔멜 게엠베하 Centrifugal separator with venturi arrangement
KR101128986B1 (en) * 2010-08-23 2012-03-23 주식회사 한국인삼공사 Centrifuge for Red Ginseng

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11314011A (en) * 1998-05-06 1999-11-16 Toshimasa Yamamoto Separation member and separation method
KR100721258B1 (en) * 2005-12-15 2007-05-23 유명기 Impurities filtration device using centrifugal force and filter
KR100680136B1 (en) * 2006-06-30 2007-02-07 메디칸(주) Centrifuge
KR20110083671A (en) * 2008-11-14 2011-07-20 만 운트 훔멜 게엠베하 Centrifugal separator with venturi arrangement
KR101128986B1 (en) * 2010-08-23 2012-03-23 주식회사 한국인삼공사 Centrifuge for Red Ginseng

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019033691A (en) * 2017-08-16 2019-03-07 ヒヨン イ Method and device for separating interstitial cell from biological tissue without use of enzyme

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