WO2016187996A1 - 循环流动式光生物反应器*** - Google Patents

循环流动式光生物反应器*** Download PDF

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Publication number
WO2016187996A1
WO2016187996A1 PCT/CN2015/090215 CN2015090215W WO2016187996A1 WO 2016187996 A1 WO2016187996 A1 WO 2016187996A1 CN 2015090215 W CN2015090215 W CN 2015090215W WO 2016187996 A1 WO2016187996 A1 WO 2016187996A1
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microalgae
photobioreactor
liquid
circulating flow
carbon dioxide
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PCT/CN2015/090215
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English (en)
French (fr)
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迈克尔·海因斯·威尔逊
约翰·格洛波
托马斯·格拉布斯
查尔斯·塞西尔
马克·克罗克
托马斯·刘
张项羽
张恒
***
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连衡会投资有限公司
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Publication of WO2016187996A1 publication Critical patent/WO2016187996A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass

Definitions

  • the invention relates to a closed photobioreactor system, in particular to a circulating flow photobioreactor system for cultivating a plurality of photosynthetic microalgae organisms by photosynthesis for industrial microalgae cultivation.
  • Photosynthetic microalgae organisms especially oil-rich microalgae, plastic microalgae, Haematococcus pluvialis, green algae, cyanobacteria, algae, etc. (abbreviated as algae) rely mainly on the corresponding minerals.
  • Single-celled algae have a strong ability to reproduce and absorb a large amount of carbon dioxide under photosynthesis.
  • algae are converted into high-value organic compounds rich in oil, protein, astaxanthin, and antioxidants by using elements such as light energy, water, minerals, and carbon dioxide.
  • a variety of high value-added products can be harvested by culturing different algal microorganisms in a photobioreactor. And because algae has the ability to rapidly absorb nutrients such as carbon dioxide, nitrogen, and phosphorus from the surrounding environment and convert it into organic compounds such as oils, proteins, and astaxanthin stored in cells, it can also be used to purify wastewater. And recycle the carbon dioxide-rich flue gas from thermal power plants to improve the environment. In the system, algae can play an important part in the biological purification process. After being matured, through systematic processes such as collection and extraction, a large amount of biofuels (such as biodiesel, alcohol and methane) can be produced, and the algae can also be used as animals. Feed and organic fertilizers, as well as important raw materials for improving the soil. In algae aquaculture, the economic benefits are determined by the choice of algae, the scale of the culture, the application efficiency of the photobioreactor design, and the cost of application.
  • nutrients such as carbon dioxide, nitrogen, and phosphorus
  • organic compounds such as oils,
  • China's large-scale photobioreactors are generally open pools, horizontal pipes, pipes, transparent light films, plastic films, etc., and open pools are built with 1000-5000 square meters of circular shallow pools (water depth). 15 to 30 cm), the culture solution is circulated by human or slurry wheel.
  • This production mode has great limitations. Its structure is simple. Although it has the advantages of low investment and easy construction, the same efficiency is too low. There are many uncontrollable factors for algae growing outdoors. For example, open pool farming lacks temperature, illumination, The mixing is uneven, the volume is small, and the contact with the air is easy to be infected, which makes the overall productivity low, often leading to failure of culture.
  • the structural unit size of the reactor can not be adjusted after determining
  • the reactor is deep, the stress distribution on the side plates is uneven, and the mechanical properties of the upper part of the material are low. At the same time, the water pressure requires high material properties of the film wall. The cost of materials increases.
  • One of the objects of the present invention is to provide a circulating flow photobioreactor system.
  • Another object of the present invention is to provide a circulating flow photobioreactor and a circulating flow photobioreactor unit.
  • a third object of the present invention is to provide a pig which can automatically clean the inner wall of a transparent pipe.
  • the circulating flow photobioreactor system of the present invention comprises an intermediate storage tank 1, a liquid operation pump 2, a recovery/drainage return pipeline 3, a recovery/return tube 4, an overflow/liquid, a gas return tube 5, and a photobioreactor 6
  • the nutrient solution prepared in the middle is placed in the intermediate storage tank 1, and the nutrient solution is transported to the lower portion of the photobioreactor 6 through the liquid operation pump 2, and the flue gas/carbon dioxide is introduced into the photobio by the gas operation pump 14.
  • the microalgae in the reactor is subjected to photosynthesis. After the photosynthesis is completed, the cultured microalgae density saturated liquid passes through the recovery/drainage returning pipe 3, passes through the recovery/return pipe 4 under the action of the liquid running pump 2, and enters through the harvesting port 7 In the harvesting tank 8, the liquid-solid separation removes the concentrated microalgae biomass product 11, and the remaining waste liquid/waste nutrient solution 9 is sterilized by the ultraviolet device 10 and returned to the intermediate storage tank 1 for reuse after nutrient preparation. The gas and liquid overflowing from the upper portion of the photobioreactor 6 are returned to the intermediate storage tank 1 through the overflow/liquid and gas return pipe 5 via the return pipe 12, and are used for nutrient preparation.
  • the photobioreactor 6 is composed of a plurality of transparent cylindrical transparent pipes arranged side by side, and the upper and lower portions of the cylindrical transparent pipe are connected through a three-way connection, each group
  • the photobioreactor unit consists of two photobioreactors 6 staggered at 45°.
  • the upper and lower portions of the two photobioreactors 6 are interconnected to form an inlet and an outlet, said transparent cylindrical transparent tubes and connections.
  • the tee is made of polyethylene terephthalate.
  • a gas delivery line is disposed in the lower communication tube of the photobioreactor 6, and the gas delivery line is densely packed for setting a certain pressure. The small hole in which the gas escapes.
  • a pig is built in each of the cylindrical transparent tubes of the photobioreactor 6.
  • Each of the pigs includes: a foam-filled cylinder, a gasket, two rubber seals, two notched disc rubber gaskets and two porous discs; the pig is foamed
  • the upper and lower sides of the cylinder are sequentially connected with a rubber sealing ring, a disc rubber gasket having a notch, a gasket, and a disk having a porous shape, which are connected by a screw and a nut.
  • the notched disc rubber gasket has several notches for allowing sufficient water to flow.
  • the angle between the upper and lower notches through which the upper and lower rubber gaskets are reserved is 45 degrees, and the gap between the upper and lower rubber gaskets is 4
  • the pig is rotated by the water flow force through which the water passes to ensure that the inner wall of the transparent pipe seamlessly cleans all of its area.
  • the lower part and the upper part of the photobioreactor unit are supported by the three-way pipe and the plurality of circulating flow pipes by the I-beam and are fixed by the buckle and the ground.
  • the buckle and the ground At 90°, there is a rubber ring between the connecting cylindrical transparent pipe and the pipe tee to prevent leakage, which is fixed by a buckle.
  • a plurality of photobioreactors The unit is connected in series, arranged in parallel with two rows of units, and the end-to-end connection extends to form a circulating flow photobioreactor.
  • the plurality of circulating flow photobioreactors are connected in parallel to form a circulating flow photobioreactor system. .
  • the photobioreactor comprises: 20-500,000 cylindrical transparent pipes and pipe tees, I-beams, connecting pieces, support frames and fixed components, and the photobioreactor units are connected in series, in two rows, in parallel, in series Together, the end-to-end connection extends to form a circulating flow photobioreactor; a plurality of circulating flow photobioreactors are then connected in parallel to form a circulating flow photobioreactor system.
  • the cylindrical transparent pipe has a height of 0.80 m to 6.00 m and a diameter of 0.05 m to 0.55 m.
  • the flow velocity is 10-20 cm/sec, and the circulatory flow photobioreactor system is introduced, and the microalgae grows.
  • the liquid stays in each cylindrical transparent pipe, uniformly passes carbon dioxide, carries out photosynthesis, and the residence time of the microalgae growth liquid is about 8-16 hours according to the microalgae seed.
  • the detector detects that the microalgae liquid is insufficiently nutritious
  • the microalgae liquid is returned to the culture solution by the inlet and outlet to prepare the storage tank, and the nutrient required for the growth of the appropriate amount of microalgae is added; and the next microalgae photobioreaction cycle is entered.
  • the microalgae growth liquid is driven into the photobioreactor by running the pump to fully absorb the photosynthetic microalgae organism, and the carbon biogas storage tank, the distribution storage tank, the harvest storage tank are equipped at the front end of the photobioreactor system device, and the first time in the deployment In the tank, the microalgae seeds and the nutrient solution for plant growth are pumped together into the photobioreactor system, and then the carbon dioxide control valve that opens into the cylindrical pipe is opened, and the carbon dioxide is added to keep the carbon dioxide in the pH setting range of the nutrient solution.
  • the microalgae plants maintain the optimal growth state for photosynthesis; according to the detection index, the microalgae plant growth liquid is returned to the mixing tank to supplement the nutrient growth of the microalgae plant.
  • the microalgae liquid reaches the set density, the microalgae liquid Enter the harvest storage tank and collect it automatically by the collector (liquid-solid separator) to obtain microalgae culture products.
  • the microalgae growth liquid enters the circulating flow photobioreactor system, and the microalgae growth liquid stays in each of the cylindrical transparent pipes, and the inlet end of the inlet of the pipe tee is connected to the intake manifold of the automatic control valve, and is opened.
  • the carbon dioxide control valve that passes into the cylindrical transparent pipe always supplements the carbon dioxide.
  • the gas control valve adjusts to increase or decrease the amount of carbon dioxide replenishment within the pH setting range. Maintaining the most appropriate solubility of carbon dioxide, allowing microalgae plants to maintain optimal growth for photosynthesis.
  • a series of probes on the flue and pH and dissolved oxygen probe 15 are used to determine the density of liquid microalgae in the intermediate storage tank, the mixing tank and the cylindrical transparent tube.
  • the carbon dioxide content in the gas phase is tracked by the carbon dioxide sensor.
  • photosynthetically active radiation can be quantified using photosensors, photon fluxes are quantified in the photosynthetic flux, and dissolved oxygen sensors are used to track the photosynthetic reaction, ensuring that there is no excess in the system. Oxygen is dissolved.
  • algae harvesting is carried out when the set density is reached, and the microalgae liquid enters the harvesting tank from the inlet and outlet, from which algae can be collected by standard means: flocculation, sedimentation, centrifugation Separation, flotation, etc. are collected by a collector to obtain a microalgae culture product.
  • the circulating flow photobioreactor comprises a gas, a liquid detector, a sensor, a running liquid pump, an air pump, an intermediate storage tank, a liquid solid separator, a photobio Reactors and components (cylindrical transparent pipes and pipe tees, support frames, connectors, etc.), liquid transfer lines, carbon dioxide gas transfer circulation lines and carbon dioxide storage tanks, liquid return manifolds, blending tanks, harvesting storage a tank, wherein: the intermediate storage tank, the preparation storage tank and the photobioreactor system are connected through a conveying pipeline, a running pump, and the cultured microalgae high-density liquid from the photobioreactor system is passed through the liquid returning pipe to the harvesting port.
  • the intermediate storage tank, the preparation storage tank and the photobioreactor system are connected through a conveying pipeline, a running pump, and the cultured microalgae high-density liquid from the photobioreactor system is passed through the liquid returning pipe to the harvesting port.
  • the solids from the separator are microalgae products for reprocessing.
  • the waste liquid enters the blending tank through the intermediate storage tank and ultraviolet sterilization, and then the seeds, nutrients, etc. are added and returned, and the amount of the photobioreactor system is controlled by the detector.
  • the carbon dioxide exhaust gas discharged from the photobioreactor system and the gas and liquid overflowing from the upper portion of the photobioreactor 6 are returned to the intermediate storage tank 1 through the overflow/liquid, gas return pipe 5 through the return pipe 12, and are used after re-nutrition preparation.
  • the amount of carbon dioxide entering the photobioreactor system is controlled by a gas detector through a carbon dioxide gas delivery loop.
  • the cycling flow photobioreactor material selection and linking components thin-walled PET tubes, commonly used as packaging materials, and American size 40 polyvinyl chloride tubes
  • the accessory constitutes the main body of the photobioreactor, the thinner cross tube on the upper part is the exhaust pipe in the system static state; the lower part is the cylindrical pipe tee, the cylindrical transparent pipe, and the lower part or the upper part of the connecting piece is made of the I-beam
  • the vertical pipe is made of prefabricated I-beam steel frame.
  • the bottom I-beams connect the circulating flow photobioreactor unit in series with the end-to-end connection, support and fix it to maintain the stability of the system; the top is also fixed by I-beam. Instead of hanging at the top.
  • the support frame is evenly arranged along the parallel pipe.
  • the height of the support frame is not less than the height of the cylindrical transparent pipe and the pipe tee; the support frame is 90 degrees perpendicular to the ground, each side is rectangular at right angles, and the vertical side of the rectangular rectangle is the support frame column, overlooking Extend the connection for the first and last connections.
  • Each of the circulating flow photobioreactor systems has a certain scale and the ability to independently culture microalgae continuously, resulting in low costs in independent operation.
  • the circulating flow light In the circulating flow photobioreactor system of the present invention, the circulating flow light
  • Each of the cylindrical transparent pipes in the bioreactor system has an automatic washer device---pipe cleaner to ensure the continuous operation of the "circulating flow” photobioreactor system, without disassembly and automatic cleaning;
  • the role of the pig is to prevent the formation of biofilm in the PET tube.
  • each trimmer will be raised to the top of the corresponding pipe row and must return to the bottom when the pipe row is empty.
  • the body part of the pig is a foam-filled cylinder (Part A), large enough to make it buoyant enough to rise and fall with the water level in the tube row.
  • Part B the edges of its two rubber seals
  • the angle between the upper and lower gaps through which the water flows from the upper and lower rubber gaskets is 45 degrees.
  • the pig is rotated under the action of the water flow force to ensure that the inner wall of the transparent pipe is cleaned seamlessly. area.
  • Each pig also has a perforated disc (Part C) at both ends to prevent the pig from leaving the tube row while allowing the microalgae growth fluid to flow in and out of the top (and bottom) of the manifold, respectively As shown in Figure 2 and Figure 3.
  • Part C perforated disc
  • the entire pig fitting is made up of a combination of screw and nut (D and E sections).
  • the circulating flow photobioreactor overcomes the lack of temperature, illumination, uneven mixing, small volume, easy dyeing, and maintenance difficulty in the prior art products.
  • the problem of almost all other photobioreactors, such as difficulty in extraction, has been realized, and automated closed culture has been realized.
  • the circulating flow photobioreactor system has a well-defined structure, each unit plate has a certain scale and the ability to continuously culture microalgae, and the cost of a single circulating flow photobioreactor is low in independent operation.
  • the circulating flow photobioreactor system of the present invention is most suitable for large scale industrialization.
  • Each of the square matrix of the circulating flow photobioreactor system is formed by combining and connecting a plurality of units.
  • the reactor is easy to disassemble and easy to transport. It can be built in any environment with sufficient light. After the construction is completed, it has a long service life under the condition of proper operation and maintenance.
  • the circulating flow type photobioreactor system of the invention has no angular edges, and the arrangement of the cylindrical transparent shaped pipes can make the algae in the closed system have no dead angle of static state, which makes cleaning and disinfection more simple and convenient.
  • the agitation of the reactor culture liquid is more sufficient and evenly distributed, thereby greatly improving the culture efficiency, the design of the independent automatic cleaning device, and the difficulty of cleaning is not increased with the increase of the height; the energy consumption is low and the cost is low during the operation.
  • the circulating flow type photobioreactor system of the invention has the advantages of long service life, large capacity and low operation cost, and is most suitable for large-scale industrialization; solving the problem of overcoming the cleaning dead angle, glass wall pressure and reaction existing in the existing reactor
  • There are several problems such as limited height of the device and inconvenient assembly and movement, resulting in high cost, low efficiency, and large-scale industrial farming of photosynthetic microalgae.
  • our company's serpentine photobioreactor system has 50% more algae growth nutrient liquid capacity.
  • Figure 1 is a schematic flow diagram of a circulating flow photobioreactor of the present invention.
  • Figure 2 is a schematic view showing the assembly of the pig of the present invention.
  • Figure 3 is a cross-sectional view showing the state of use of the pig of the present invention in a pipe.
  • Figure 4 is a schematic view showing the connection of the circulating flow photobioreactor unit of the present invention.
  • Figure 5 is a schematic diagram of measuring pH and set points.
  • Figure 6 is a schematic diagram of dissolved oxygen related production and PAR.
  • Figure 7 is a schematic view showing the circular pipe connection of the circulating flow photobioreactor of the present invention.
  • symbol 1 represents an intermediate storage tank
  • symbol 2 represents a liquid running pump
  • symbol 3 represents a recovery/drainage return pipe
  • symbol 4 represents a recovery/return pipe
  • symbol 5 represents an overflow/liquid
  • symbol 6 represents Photobioreactor
  • symbol 7 represents the harvesting port
  • symbol 8 represents the harvesting tank
  • symbol 9 represents the waste liquid/waste nutrient solution
  • symbol 10 represents the ultraviolet disinfection device
  • symbol 11 represents the concentrated biomass product
  • symbol 12 represents the return tube
  • symbol 13 represents a flue gas/carbon dioxide introduction device
  • symbol 14 represents a gas running pump
  • symbol 15 represents a flue and a pH value and a dissolved oxygen probe.
  • symbol A represents a cylinder filled with foam
  • symbol B represents a rubber seal
  • symbol C represents a disc having a notch
  • symbol D represents a screw
  • symbol E represents a nut.
  • symbol 16 represents photobioreactor A
  • symbol 17 represents photobioreactor B
  • symbol 18 represents a connector
  • symbol 19 represents a connecting tube.
  • the cylindrical transparent pipe and the pipe tee are made of polyethylene terephthalate, and the microalgae growth liquid is prepared in the mixing tank, and the flow rate is 10-20 cm/sec.
  • the microalgae growth solution stays in each cylindrical transparent pipe, and uniformly passes carbon dioxide for photosynthesis; the residence time of the microalgae growth solution is about 6-12 depending on the microalgae seed.
  • the hour is a cycle; return to the microalgae growth liquid pool to supplement the nutrients needed for the growth of photosynthetic microalgae, and to remove harmful substances.
  • the material selection and linking components thin-walled PET tubes, commonly used as packaging materials,
  • the main body of the photobioreactor is made up of the American specification 40 polyvinyl chloride pipe fitting.
  • the thinner horizontal pipe on the upper part is the exhaust pipe in the static state of the system; the lower part is the cylindrical pipe tee, the cylindrical transparent pipe, the connecting piece
  • the lower part or the upper part uses the I-beam to support and fix the plurality of circulating flow pipes in parallel, parallel, and two rows of rows, and the first and last joints extend and communicate.
  • a properly sized rubber band is used to create a gasket between the pipe and the fitting.
  • the vertical pipe is made of prefabricated I-beam steel frame.
  • the bottom I-beam extends the parallel connection of the head and tail connections, supports and fixes it to maintain the stability of the system; the top is also fixed by the I-beam instead of hanging at the top.
  • carbon dioxide is injected according to the pH of the system.
  • carbonic acid is formed (carbon dioxide is dissolved in water to form carbonic acid), which in turn reduces the pH of the algae.
  • the microalgae organisms are photosynthesized, the dissolved carbon dioxide is consumed and the pH of the system rises.
  • the nutrient gas contains other acidic flue gas components such as SOx and NOx.
  • the SOx solution forms sulfuric acid, which causes the medium to be acidified, thereby inhibiting growth. For this reason, SOx will not be added to the culture system utilized by algae.
  • Figure 5 depicts the method of controlling the pH for adjusting the carbon dioxide flow of algae in a 650 liter pipeline over a six day period.
  • the horizontal line represents the pH set point of the reactor and the trace line represents the pH value measured by the system. This figure also captures the occurrence of carbon dioxide-producing respiration, thereby reducing the pH at night.
  • the oxygen inhibition rate can interfere with limiting the productivity of algae by the ability of the essential components in algae cells to absorb carbon dioxide.
  • the problem becomes very small because carbon dioxide, as a gas after combustion, is 80% less oxygen than air.

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Abstract

提供了一种循环流动式光生物反应器***，其包括中间贮槽1、液体运行泵2、回收/引流回归管道3、回收/回归管4、溢出液/气体回归管5、光生物反应器6、收获口7、收获储罐8、废液体/废营养液9、紫外线杀菌装置10、浓缩的生物量产品11、返回管12、烟道气/二氧化碳引进装置13、气体运行泵14、烟道和pH值和溶解氧探针15。所述循环流动式光生物反应器***实现了自动化封闭式养殖。

Description

循环流动式光生物反应器*** 技术领域
本发明涉及一种封闭式光生物反应器***，特别的涉及一种通过光合作用培养多种光合微藻生物，进行产业化微藻养殖的循环流动式光生物反应器***。
背景技术
光合微藻生物，尤其是富油能源微藻、塑料微藻、雨生红球藻、绿藻、蓝藻、球藻等(简称藻类)的生长繁殖主要依靠相应的矿物质。单细胞的藻类有着极强的繁殖能力，在光合作用下可以吸收大量的二氧化碳。藻类通过光合作用，利用光能、水、矿物质和二氧化碳等元素转化成富含大量油脂、蛋白质、虾青素、抗氧化等高附价值有机化合物。
通过光生物反应器养殖不同的藻类微生物，可以收获多种不同的高附加值产品。并且由于藻类具有快速从周围环境中吸收二氧化碳、氮、磷等营养物质并将其转化为有机化合物(如储存在细胞中的油脂、蛋白、虾青素)的能力，它还可以用于净化废水和回收利用火电厂富含二氧化碳的烟道气来改善环境。在***中藻类可以起到生物净化过程中的重要部分，待成熟以后，经过收集、提取等***工艺，产出大量生物燃料(如生物柴油，酒精和甲烷等)、其藻体也可作为动物饲料和有机肥料，以及改良土壤的重要原料。在藻类水产养殖中，其产生的经济效益是由藻类的选择、养殖的规模、光生物反应器设计的应用效率和应用成本来决定的。
目前国内有规模的光生物反应器一般为开放池、横管、管道、透明光膜、塑料膜等，开放池多建成1000～5000平方米环形的浅池(水深 15～30厘米)，用人力或浆轮使培养液循环流动。这种生产模式具有很大的局限性，其结构简单，虽然有投资低易建设的优点，但同样的效率过于低下，藻类在户外生长有很多不可控因素，如开放池养殖缺少温度、光照、混合不均匀、容积小，与空气接触易于染菌，使得总体生产率低，常导致培养失败。
开放式的缺陷推动了封闭式培养***的发展，用透明的管道、平板式或其它容器制成的封闭式光生物反应器出现了。横管式、平板式反应器的应用也受到其高成本、效率低的限制，仅用于生产小批量、高附加值特种产品；其它种类的光生物反应器也因多种原因如提取困难、建设成本高、容积小、清洗困难等而相继无法大规模养殖产生经济效益，限制了微藻产业的发展规模。
专利文件(公开号WO 2008/151376A1)公开了一种薄膜反应器，但是，该薄膜反应器也存在如下缺点：
a、反应器的结构单元尺寸确定后无法调整；
b、制作工艺较为复杂，每个薄膜单元侧面呈波浪状，需要烫接或其他辅助工艺；
c、由于反应器宽度较小，同时波浪状侧壁导致内部贯通性较差，有死角，清洗很不方便；
d、由于该反应器侧面是主要受光面，反应器摆放形式(方向)受到限制；
e、反应器较深，侧板上的应力分布不均，材料上部力学性能利用率低。同时，水压对薄膜壁的材料性能要求较高。材料成本随之升高。
发明内容
本发明的目的之一是提供一种循环流动式光生物反应器***。
本发明的目的之二是提供一种循环流动式光生物反应器和循环流动式光生物反应器单元。
本发明的目的之三是提供一种可以自动清洗透明管道内壁的清管器。
本发明的这些以及其它目的将通过下列详细描述和说明来进一步阐述。
发明内容
本发明的循环流动式光生物反应器***，包括中间贮槽1、液体运行泵2、回收/引流回归管道3、回收/回归管4、溢出/液、气体回归管5、光生物反应器6、收获口7、收获储罐8、废液体/废营养液9、紫外线杀菌装置10、浓缩的生物量产品11、返回管12、烟道气/二氧化碳引进装置13、气体运行泵14、烟道和PH值和溶解氧探针15。其中将调配好的营养液放入中间储罐1中，通过液体运行泵2将营养液输送到光生物反应器6的下方进入，同时通过气体运行泵14将烟道气/二氧化碳引进在光生物反应器中的微藻进行光合作用，光合作用完成后，养殖微藻密度饱和液通过回收/引流回归管道3，在液体运行泵2的动力作用下通过回收/回归管4，经收获口7进入收获储罐8中，液固分离移出浓缩的微藻生物量产品11，余下的废液体/废营养液9经过紫外线装置10杀菌后返回中间贮槽1中重新进行营养调配后使用。从光生物反应器6的上部溢出的气体和液体通过溢出/液、气体回归管5经返回管12也返回中间贮槽1中重新进行营养调配后使用。
在本发明的循环流动式光生物反应器***中，所述的光生物反应器6有并排的多个透明圆柱透明形管道构成，圆柱形透明管道的上部和下部通过三通连接贯通，每组光生物反应器单元有二个交错排列成45°的光生物反应器6组成，二个光生物反应器6的上部和下部相互连接成一个进口和出口，所述的透明圆柱透明形管道和连接三通是由聚对苯二甲酸乙二醇酯制成。
在本发明的循环流动式光生物反应器***中，在所述的光生物反应器6的下部连通管中有一气体输送管线，所述的气体输送管线上密布多个用于在设定一定压力的状态下气体逸出的小孔。
在本发明的循环流动式光生物反应器***中，在所述的光生物反应器6的每个圆柱透明形管道中均内置安装有清管器。所述的每个清管器包括:充满泡沫的圆柱体、垫片、两个橡胶密封圈，两个具有缺口的圆盘橡胶垫片和两个具有多孔的圆盘；清管器是泡沫的圆柱体的上下均依次连接橡胶密封圈、具有缺口的圆盘橡胶垫片、垫片和具有多孔的圆盘，通过螺杆和螺母连接成型。具有缺口的圆盘橡胶垫片上有几个允许足够水流通的缺口，上下橡胶垫片预留的水流通过的上下缺口之间的夹角为45度，上下橡胶垫片的缺口均为4个，在水通过的水流速力的作用下使清管器旋转，以确保透明管道内侧管壁无缝清洗其所有面积。
在本发明的循环流动式光生物反应器***中，所述的光生物反应器单元的下部、上部用工字钢把管道三通和多个循环流动管道支撑并通过卡扣固定下来，并与地面成90°，连接件间圆柱透明形管道和管道三通间有防止泄露的橡胶圈，通过卡扣来固定。
在本发明的循环流动式光生物反应器***中，多个光生物反应器单 元串联，以两行为单位、平行排列，俯视首尾连接延伸相通，组成了循环流动式光生物反应器，多个循环流动式光生物反应器再并联在一起组成了循环流动式光生物反应器***。所述的光生物反应器：由20-500000个圆柱形透明管道和管道三通、工字钢、连接件、支撑架并固定组成光生物反应器单元串联成行、两行为单位、平行排列，串联在一起，俯视首尾连接延伸相通，组成循环流动式光生物反应器；多个循环流动式光生物反应器再并联在一起组成了循环流动式光生物反应器***。圆柱形透明管道高度为0.80米-6.00米，直径为0.05米-0.55米。
在本发明的循环流动式光生物反应器***中，微藻生长液在调配罐中调配好后，以流动速度为10-20厘米/秒，进入循环流动式光生物反应器***，微藻生长液停留在每个圆柱形透明管道，均匀的通入二氧化碳，进行光合作用，微藻生长液停留时间，根据微藻种子的不同，约为8-16小时为一个循环周期。探测器当检测到微藻液体营养不足时，由进出口把微藻液体回到培养液调配储罐、补充适量的微藻生长所需要的营养物质；进入下一个微藻光生物反应循环。
通过运行泵将微藻生长液打入光生物反应器，使光合微藻生物充分吸收，在光生物反应器***装置的前端配有二氧化碳储气罐、调配储罐、收获储罐，首次在调配罐中把微藻种子、植物生长的营养液一起泵入光生物反应器***，再开启通入圆柱形管道的二氧化碳控制阀，一直补充二氧化碳，保持着二氧化碳在营养液中pH值设定范围内，让微藻植物保持最佳生长状态进行光合作用；根据检测指标把微藻植物生长液及时返回到调配罐中补充微藻植物生长的营养，当微藻液达到设定密度时，微藻液体进入收获储罐，由收集器(液固分离机)会自动收集，获得微藻养殖产品。
微藻生长液进入循环流动式光生物反应器***，微藻生长液停留在每个圆柱形透明管道里，管道三通下部的进气孔进入端连接到自动控制阀的进气歧管，开启通入圆柱透明形管道的二氧化碳控制阀，一直补充二氧化碳，当浆料的pH值高于或低于设定范围，气体控制阀调节增加或减少二氧化碳补充的量，在pH值设定范围内，保持着二氧化碳最适当溶解度，让微藻植物保持最佳生长状态进行光合作用。
用烟道和PH值和溶解氧探针15上一系列的探针来测定中间贮槽、调配罐与圆柱透明形管道中的液体微藻密度，在气相中的二氧化碳含量是由二氧化碳传感器跟踪，以确保***有足够的二氧化碳去引导藻类的生长，光合有效辐射可以使用量子传感器，测量光子在光合谱通量量化，溶解氧传感器是用来跟踪的光合反应的产物，确保在***中没有过量的氧气溶解。
在本发明的循环流动式光生物反应器***中，当达到设定密度时进行藻类收获，微藻液体由进出口进入收获储罐，从这里可以通过标准方式收集藻类：如絮凝、沉淀，离心分离，浮选等由收集器收集，获得微藻养殖产品。
在本发明的循环流动式光生物反应器***中，所述的循环流动式光生物反应器包括气、液体探测器、传感器、运行液泵、气泵、中间贮槽、液固分离机、光生物反应器及组件(圆柱透明形管道和管道三通、支撑架、连接件等)、液体输送管路、二氧化碳气体输送循环管路和二氧化碳储气罐、回液岐管、调配储罐、收获储罐，其中：通过输送管路、运行泵将中间贮槽、调配储罐和光生物反应器***连接，从光生物反应器***出来的养殖微藻高密度液，通过回液岐管到收成口进入收获储罐；经絮凝、液固 分离机出来的固体为微藻产品用于再加工，废液体通过中间贮槽和紫外线杀菌后进入调配储罐再进行种子、养分等添加归还，通过探测器来控制进入光生物反应器***的量；从光生物反应器***排出来的二氧化碳废气和从光生物反应器6的上部溢出的气体和液体通过溢出/液、气体回归管5经返回管12返回中间贮槽1中重新营养调配后使用；经二氧化碳气体输送循环管路，通过气体探测器来控制进入光生物反应器***二氧化碳的量。
在本发明的循环流动式光生物反应器***中，所述的循环流动式光生物反应器材料的选择和链接组件：薄壁PET管，常用为包装的材料，与美国规格40聚氯乙烯管配件组成光生物反应器的主体，上面的较细的横管是***静止状态下的排气管；下面是圆柱形管道三通、圆柱透明形管道，连接件的下部或上部用工字钢把管道三通把多个循环流动管道支撑并固定下来，循环流动式光生物反应器单元串联、平行、两行为单位排列，俯视首尾连接延伸相通。为了保证密封不漏水，适当大小的橡皮圈被用作创建管道和配件之间的垫片，使用一个蜗杆传动卡箍加固密封。垂直管道是通过预制的工字钢框架，底部工字钢把循环流动式光生物反应器单元串联俯视首尾连接延伸相通，支撑并固定下来，保持***的稳定性；顶部也是由工字钢固定，而不是悬挂在顶部。支撑框架沿平行管道管均匀布设，支撑框架的高不小于圆柱透明形管道及管道三通的高；支撑框架垂直地面90度，每侧呈直角长方形，直角长方形的垂直边为支撑框架立柱，俯视为首尾连接延伸相通。每一个循环流动式光生物反应器***都具有一定的规模和独立连续培养微藻的能力，在独立运行中产生的费用低。
在本发明的循环流动式光生物反应器***中，所述的循环流动式光 生物反应器***内的每一个圆柱透明形管道里，均内置一个自动清洗器装置---清管器，保证了“循环流动”光生物反应器***的连续性操作，无拆卸自动清洗；
清管器的作用是防止在PET管内有生物膜的形成。为了完成这项任务，当管道内注满了水时，每个清官器将提升到相应的管排的顶端，并且在管排是空的时候必须回到底部。清管器的身体部分是充满泡沫的圆柱体(A部分)，足够大的体积使它的浮力足以令其在管排内随着水位上升与下降。随着清管器的上升(和下降)，它的两个橡胶密封圈的边缘(B部分)移除所有生长在PET管墙的藻类。然而，虽然这些垫片必须与管排的边缘保持接触，但是它们也必须不能限制清管器周边的水流。为了防止这样的限制，每个垫片都应有几个允许足够水流通过的缺口。上下橡胶垫片预留的水流通过的上下缺口之间的夹角为45度，当水通过时，在水流速力的作用下使清管器旋转，以确保透明管道内侧管壁无缝清洗其所有面积。每个清管器在两头还具有一个多孔的圆盘(C部分)以防止清管器脱离管排的同时还能使微藻生长液在歧管的顶部(和底部)流进与流出，分别如图2和图3所示。最后，整个清管器的配件是由螺杆和螺母组在一块的(D跟E部分)。
在本发明的循环流动式光生物反应器***中，所述的循环流动式光生物反应器克服了现有技术中产品缺少温度、光照、混合不均匀、容积小、易染菌、维护困难，提取困难等几乎全部其它光生物反应器出现的问题，实现了自动化封闭式养殖。循环流动式光生物反应器***结构分明，每一个单元板块都具有一定的规模和连续培养微藻的能力，单一的循环流动式光生物反应器独立运行中产生的费用低。
在本发明的循环流动式光生物反应器***中，所述的循环流动式光生物反应器***最适合大规模产业化。循环流动式光生物反应器***每个方阵为多个单元进行组合连接而成，随着土地的大小规模变化，可以分批逐次扩建运行。反应器拆卸简单，方便运输，可在任何光照充足的环境中搭建，搭建完成后在运行和维护得当的情况下使用寿命很长。
本发明的循环流动式光生物反应器***不存在棱角，其圆柱透明形管道的排列连接方式，可以让封闭***内的藻类没有静止状态的死角，这使得清洗、消毒变的更加简单、便捷，反应器培养液的搅动更加充分和分布均匀，从而大大提高了培养效率，独立的自动清洗装置设计，且随着高度的增加也不会增加清洗难度；在运行过程中消耗能源少、费用低，并且除了圆柱形管道三通连接处以外，全部采用透光性的聚对苯二甲酸乙二醇酯制成。
本发明的循环流动式光生物反应器***集使用寿命长、容量大、运行成本低，最适宜大规模产业化于一身；解决克服了已有反应器还存在的清洗死角、玻璃壁压力、反应器高度受限、组装移动不方便等若干问题，从而导致的光合微藻生物培养成本高、效率低，无法大规模产业化养殖的问题。是最适宜微藻水产养殖产业化规模化的自动化封闭式循环流动式光生物反应器***；特别是容积量比普通封闭式光生物反应器藻类生长营养液体容量高10-20倍之多，较前本公司的蛇形光生物反应器***的藻类生长营养液体容量多50％。
附图说明
图1是本发明循环流动式光生物反应器流程示意图。
图2是本发明清管器装配示意图。
图3是本发明清管器在管道中使用状态的剖视图。
图4是本发明循环流动式光生物反应器单元连接示意图。
图5是测量pH值与设置点示意图。
图6是溶解氧相关生产与PAR示意图。
图7是本发明循环流动式光生物反应器圆形管道连接示意图。
在图1中，符号1代表中间贮槽，符号2代表液体运行泵，符号3代表回收/引流回归管道，符号4代表回收/回归管，符号5代表溢出/液，气体回归管，符号6代表光生物反应器，符号7代表收获口，符号8代表收获储罐，符号9代表废液体/废营养液，符号10代表紫外线杀菌装置，符号11代表浓缩的生物量产品，符号12代表返回管，符号13代表烟道气/二氧化碳引进装置，符号14代表气体运行泵，符号15代表烟道和PH值和溶解氧探针。
在图2，图3中，符号A代表充满泡沫的圆柱体，符号B代表橡胶密封圈，符号C代表具有缺口的圆盘，符号D代表螺杆，符号E代表螺母。
在图4中，符号16代表光生物反应器A，符号17代表光生物反应器B，符号18代表连接头，符号19代表连接管。
以下结合附图对本发明进行详细描述
参考图1，圆柱透明形管道和管道三通由聚对苯二甲酸乙二醇酯制成，微藻生长液在调配罐中调配好后，以流动速度为10-20厘米/秒，进入循环流动式光生物反应器***，微藻生长液停留在每个圆柱形透明管道，均匀的通入二氧化碳，进行光合作用；微藻生长液停留时间，根据微藻种子的不同，约为6-12小时为一个循环周期；回到微藻生长液池中补充光合微藻生物生长所需要的营养物质，以及清除有害物质。
参考图7，材料的选择和链接组件：薄壁PET管，常用为包装的材料， 与美国规格40聚氯乙烯管配件组成光生物反应器的主体，上面的较细的横管是***静止状态下的排气管；下面是圆柱形管道三通、圆柱透明形管道，连接件的下部或上部用工字钢把管道三通把多个循环流动管道支撑并固定下来并联、平行、两行为单位排列，俯视首尾连接延伸相通。为了保证圆柱透明形管道与管道三通连接密封不漏水，适当大小的橡皮圈被用作创建管道和配件之间的垫片。使用一个蜗杆传动卡箍加固密封。垂直管道是通过预制的工字钢框架，底部工字钢把并联俯视首尾连接延伸相通，支撑并固定下来，保持***的稳定性；顶部也是由工字钢固定，而不是悬挂在顶部。
参考图5，根据***的pH值注入二氧化碳。当二氧化碳注入到反应器，就会形成碳酸(二氧化碳溶解于水生成碳酸)，继而降低藻类的pH值。当微藻生物经光合作用后，溶解的二氧化碳被消耗掉，***的pH值上升。这种方式能使***的pH值保持在最适合藻类生长的范围值(pH＝4.2-6.2)，同时又能提供足够的二氧化碳以维持生长。这点十分重要，尤其是养料气体里含有其他酸性烟气成分如SOx和NOx。尤其是SOx的溶解液会形成硫酸，使培养基过酸化，从而抑制生长。出于这个原因，SOx将不被加入到藻类利用的培养***。
图5描述了控制pH值的办法，用于调节六天内650公升管道的海藻的二氧化碳流量。水平线是表示反应器的pH设定点，而痕迹线表示的是***测量的pH值。此图还捕捉到产生二氧化碳的呼吸的发生，从而降低在夜间的pH值。
参考图6，除了pH值，还有很多不同的方式在运行过程当中不断监测跟踪光生物反应器的性能与藻类的健康。这些测量方式(包括pH值) 通过一系列的探针监控养料罐与PBR间的流动液体。环境与过程的温度都是经过***性能与环境影响相关的测量。在气相中的二氧化碳含量是由二氧化碳探测器跟踪，以确保***有足够的二氧化碳去引导藻类的生长。如果需要，光合有效辐射(PAR)可以使用量子传感器，测量光子在光合谱通量量化。溶解氧传感器是用来跟踪的光合反应的产物，确保在***中没有过量的氧气溶解。氧气的产生等同于二氧化碳的消耗。因此，通过监控溶解氧，可以确认藻类的表现与健康。图6显示了在光生物反应器里的溶解氧与PAR值的关系。
值得注意的是，氧气抑制率可以通过在藻类细胞中的基本组分吸收二氧化碳的能力干扰限制藻类的生产力。然而，当使用烟道气的二氧化碳源时，问题就变得很小了，因为二氧化碳作为燃烧后的气体，其相较于空气少80％的氧含量。

Claims (15)

  1. 一种循环流动式光生物反应器***，其特征在于包括中间贮槽(1)、液体运行泵(2)、回收/引流回归管道(3)、回收/回归管(4)、溢出/液、气体回归管(5)、光生物反应器(6)、收获口(7)、收获储罐(8)、废液体/废营养液(9)、紫外线杀菌装置(10)、浓缩的生物量产品(11)、返回管(12)、烟道气/二氧化碳引进装置(13)、气体运行泵(14)、烟道和PH值和溶解氧探针(15)；其中将调配好的营养液放入中间储罐(1)中，通过泵(2)将营养液输送到光生物反应器(6)的下方进入，同时通过气体运行泵(14)将净化烟道气/二氧化碳引进在光生物反应器中的微藻进行光合作用，光合作用完成后，养殖微藻密度饱和液通过回收/引流回归管道(3)，在液体运行泵(2)的动力作用下通过回收/回归管(4)，从收获口(7)进入收获储罐(8)中，液固分离移出浓缩的微藻生物量产品(11)，余下的废液体/废营养液(9)经过紫外线装置(10)杀菌后废营养液返回中间贮槽(1)中重新营养调配后使用；从光生物反应器(6)的上部溢出的气体和液体通过溢出/液、气体回归管(5)经返回管(12)也返回中间贮槽(1)中重新营养调配后使用。
  2. 根据权利要求1所述的循环流动式光生物反应器***，其特征在于所述的光生物反应器(6)有并成两排的多个透明圆柱透明形管道构成，圆柱透明形管道的上部和下部通过三通连接贯通，每组光生物反应器单元有二个交错排列成45°的光生物反应器(6)组成，二个光生物反应器(6)的上部和下部相互连接成一个进口和出口。
  3. 根据权利要求1所述的循环流动式光生物反应器***，其特征在于所述的光生物反应器(6)的下部连通管中有一气体输送管线，所述的气体输送管线上密布多个用于气体逸出的小孔。
  4. 根据权利要求1所述的循环流动式光生物反应器***，其特征在于在所述的光生物反应器(6)的每个圆柱透明形管道中内置安装有清管器。
  5. 根据权利要求4所述的循环流动式光生物反应器***，其特征在于所述的在每个圆柱透明形管道中内置的清管器：包括充满泡沫的圆柱体、垫片、两个橡胶密封圈，两个具有缺口的圆盘橡胶垫片和两个具有多孔的圆盘。
  6. 根据权利要求5所述的循环流动式光生物反应器***的每个圆柱透明形管道中内置安装有清管器，其特征在于充满泡沫的圆柱体的上下均依次连接橡胶密封圈、具有缺口的圆盘橡胶垫片、垫片和具有多孔的圆盘，通过螺杆和螺母连接成型。
  7. 根据权利要求5或6所述的循环流动式光生物反应器***的每个圆柱透明形管道中内置安装有清管器，其特征在于具有缺口的圆盘橡胶垫片上有几个允许足够水流通的缺口，上下橡胶垫片预留的水流通过的上下缺口之间的夹角为45度，在水通过的水流速力的作用下使清管器旋转，以确保透明管道内侧管壁无缝清洗其所有面积。
  8. 根据权利要求1所述的循环流动式光生物反应器***，其特征在于所述的光生物反应器单元：下部、上部用工字钢把管道三通和多个循环流动透明管道支撑并通过卡扣固定下来，并与地面成90°，连接件间圆柱透明形管道和管道三通间有防止泄露的橡胶圈，通过卡扣来固定。
  9. 根据权利要求8所述的循环流动式光生物反应器***，其特征在于所述的光生物反应器：由20-500000个圆柱形透明管道和管道三通、工字钢、连接件、支撑架并固定组成光生物反应器单元串联成行、平行、两行为单位排列，串联在一起，俯视首尾连接延伸相通，组成循环流动式光生 物反应器；圆柱形透明管道高度为0.80米-6.00米，直径为0.05米-0.55米。
  10. 根据权利要求9所述的循环流动式光生物反应器***，其特征在于多个光生物反应器单元串联成行、平行、两行为单位排列，串联在一起，俯视首尾连接延伸相通，组成循环流动式光生物反应器；多个循环流动式光生物反应器，再并联在一起组成了循环流动式光生物反应器***。
  11. 根据权利要求1-10所述的循环流动式光生物反应器***，其特征在于微藻生长液在调配罐中调配好后，以流动速度为10-20厘米/秒，进入循环流动式光生物反应器***，微藻生长液停留在每个圆柱形透明管道，均匀的通入二氧化碳，进行光合作用，微藻生长液停留时间，根据微藻种子的不同，约为8-16小时为一个循环周期。探测器当检测到微藻液体营养不足时，由进出口把微藻液体回到培养液调配储罐、补充适量的微藻生长所需要的营养物质；进入下一个微藻光生物反应循环。
  12. 根据权利要求11所述的循环流动式光生物反应器***，其特征在于通过液体运行泵将微藻生长液打入光生物反应器***，使光合作用微藻生物充分吸收；在光生物反应器***装置的前端配有二氧化碳储气罐、调配储罐、中间储罐、收获储罐，首次在调配罐中把微藻种子、植物生长的营养液一起泵入光生物反应器***，再开启通入圆柱透明形管道的二氧化碳控制阀，一直补充二氧化碳，保持着二氧化碳在营养液中pH值设定范围内，让微藻植物保持最佳生长状态进行光合作用；根据检测指标把微藻植物生长液及时返回到调配罐中补充微藻植物生长的营养，当微藻液达到设定密度时，微藻生长液体进入收获储罐，由收集器会自动收集，获得微藻养殖产品。
  13. 根据权利要求12所述的循环流动式光生物反应器***，其特征在于 微藻生长液进入循环流动式光生物反应器***，微藻生长液停留在每个圆柱形透明管道里，管道三通下部的进气孔进入端连接到自动控制阀的进气歧管，开启通入圆柱透明形管道的二氧化碳控制阀，一直补充二氧化碳，当浆料的pH值高于或低于设定范围，气体控制阀调节增加或减少二氧化碳补充的量，在pH值设定范围内，保持着二氧化碳最适当溶解度，让微藻植物保持最佳生长状态进行光合作用。
  14. 根据权利要求13所述的循环流动式光生物反应器***，其特征在于用烟道和PH值和溶解氧探针15上一系列的探针来测定中间贮槽、调配罐与圆柱透明形管道中的液体微藻密度，在气相中的二氧化碳含量是由二氧化碳传感器跟踪，以确保***有足够的二氧化碳去引导藻类的生长，光合有效辐射可以使用量子传感器，测量光子在光合谱通量量化，溶解氧传感器是用来跟踪的光合反应的产物，确保在***中没有过量的氧气溶解。
  15. 根据权利要求1所述的循环流动式光生物反应器***，其特征在于当达到设定密度时进行藻类收获，微藻液体由进出口进入收获储罐，从这里可以通过标准方式集中藻类，如絮凝、沉淀，离心分离，浮选等由收集器收集，获得微藻养殖产品。
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