WO2016182403A1 - Additif alimentaire comprenant un dérivé de lysine - Google Patents
Additif alimentaire comprenant un dérivé de lysine Download PDFInfo
- Publication number
- WO2016182403A1 WO2016182403A1 PCT/KR2016/005118 KR2016005118W WO2016182403A1 WO 2016182403 A1 WO2016182403 A1 WO 2016182403A1 KR 2016005118 W KR2016005118 W KR 2016005118W WO 2016182403 A1 WO2016182403 A1 WO 2016182403A1
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- WIPO (PCT)
- Prior art keywords
- acl
- rumen
- feed
- lysine
- feed additive
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-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
Definitions
- the present application relates to a feed additive comprising a lysine derivative and a method for raising animals using the same.
- ACL ⁇ -amino- ⁇ -caprolactam
- One object of the present application is to provide a feed additive comprising ⁇ -amino- ⁇ -caprolactam (ACL) or a salt thereof as an active ingredient.
- ACL ⁇ -amino- ⁇ -caprolactam
- Another object of the present application is to provide a feed composition comprising the feed additive.
- Another object of the present application is to provide a method of raising an animal, comprising feeding the feed composition to the animal.
- ⁇ -amino- ⁇ -caprolactam is a lysine derivative, which has a low rate of being degraded and absorbed by ruminant microorganisms, so that it can be absorbed in the stomach or small intestine of ruminants, resulting in an increase in ruminants and improvement of oil quality. .
- FIG. 1 is a diagram showing a scheme for generating ⁇ -amino- ⁇ -caprolactam (ACL) from lysine.
- ACL ⁇ -amino- ⁇ -caprolactam
- Figure 2 is a diagram showing the total gas generation amount (ml) of each of the control, lysine, L-ACL, and D / L-ACL.
- Fig. 3 is a diagram showing the results of confirming the ratios of cattle rumen bypass by lysine, L-ACL, and D / L-ACL.
- One embodiment embodying the present application is a feed additive, comprising ⁇ -amino- ⁇ -caprolactam (ACL) or a salt thereof as an active ingredient.
- ACL ⁇ -amino- ⁇ -caprolactam
- ⁇ -amino- ⁇ -caprolactam means a compound represented by the following Chemical Formula 1.
- the ACL is one of the lysine derivatives, also named lysine lactams.
- Preparation of the ACL can be carried out using a variety of methods known in the art, for example, it can be carried out by enzymatic synthesis or chemical synthesis. More specifically, it can be synthesized using a ring closure reaction for converting lysine to ACL, using a catalyst (catalyst) for converting lysine to ACL, or using an appropriate organic solvent for converting lysine to ACL. It can be prepared by, but is not limited to those described above.
- the ACL may be included in the feed additive in the form of its salt.
- the ACL may be provided as a salt in an acceptable form as a feed ingredient, which may be prepared by known methods.
- specific examples include inorganic acids such as hydrochloric acid, nitric acid, phosphoric acid and sulfuric acid; Sulfonic acids such as methanesulfonic acid; Or acid addition salts formed by organic carbon acids such as oxalic acid, acetic acid, fumaric acid, malonic acid, maleic acid, malic acid, succinic acid, but are not limited thereto.
- the ACL may be in the form of L-ACL, D-ACL or D / L-ACL, but is not limited thereto.
- lysine is one of the basic ⁇ -amino acids, which, together with methionine and cysteine, belong to the first limited amino acid of ruminants, and lysine is biosynthesized from oxalacetic acid via the lysine biosynthetic pathway.
- feed additive means a substance added to a feed composition.
- the feed additive may be to improve productivity or health of the target animal, but is not limited thereto.
- the feed additive may correspond to auxiliary feed in the feed management method.
- the feed additive may further include nutrients such as nucleotides, amino acids, calcium, phosphoric acid, organic acids, etc. to increase productivity or health of the target animal, but is not limited thereto.
- feed composition refers to food to be given to animals.
- the feed composition refers to a substance that supplies organic or inorganic nutrients necessary for maintaining the life of an animal or producing meat, milk, and the like.
- the feed composition may include a feed additive, and may further include nutritional ingredients necessary for maintaining the life of the animal, or producing meat, milk, and the like.
- the individual to which the feed additive or feed composition including the same is applicable is not particularly limited, and any form may be applied.
- the present invention may be applied to animals such as cattle, sheep, giraffes, camels, deer, goats, and the like without limitation, and specifically, but not limited to ruminants having rumen.
- ruminant is a special digestive tract found in some animals of mammalian joiners, and is divided into four rooms, called hump, honeycomb, folds, and wrinkles, for the purpose of rubbing. Also known as ruminwiwi, once swallowed food into the mouth again to chew well swallow swallowing, this rumen is called the stomach to enable rumination. In the rumen, microbial symbiosis has the ability to decompose and energize the cellulose of plants that cannot be digested by ordinary animals.
- ruminant refers to an animal having the rumen described above, which includes the animals of the family Camel, Baby Deer, Deer, Giraffe and Bovine.
- bovine animals include, for example, cattle, goats, black goats, sheep, and the like, and more specifically, cattle. However, it is not limited thereto.
- the feed additive according to the present application may be used individually, may be used in combination with a conventionally known feed additive, and may be used sequentially or simultaneously with the conventional feed additive.
- Another embodiment embodying the present application is a feed composition comprising the feed additive.
- the feed additive and feed composition are as described above.
- the feed composition may be paid according to a general specification management method, may be paid in the morning and afternoon, but is not limited thereto.
- the amount of salary is not particularly limited.
- Another aspect of embodying the present application is a method of raising an animal, comprising feeding the feed additive or feed composition to the animal.
- the method may be a method comprising feeding the feed composition to a ruminant.
- the method may be paid according to a general specification management method, and may be paid in the morning and afternoon, but is not limited thereto.
- the amount of salary is not particularly limited.
- Holstein (Holstein) castor with rumen cannula (with weight of 630 ⁇ 650kg) was released and two dogs were fed twice a day (7:30 am, 3:00 pm).
- Commercial feed (CJ CheilJedang, MilkzenTM) And rice straw was fed by breeding.
- the rumen fluid was collected around 10 am on the day of the experiment, and the contents of the rumen were taken out through the cannula, squeezed out with gastric fluid with gauze, and then packed in a thermos bubbling with CO 2 and transported to the laboratory in a state of blocking oxygen invasion. It was used later. It took less than an hour to transport to the lab.
- McDougall's buffer (Troelsen and Donna, 1966), which is generally used in rumen experiments after filtration with two layers of gauze, and used as an anaerobic culture solution.
- Table 1 The composition of McDougall's buffer simulations is shown in Table 1.
- Test feed was used as a commercial feed (CJ Cheil Jedang, Milk JenTM) as a basic feed, and the test material was prepared by mixing the test material with the basic feed.
- Test materials were used as L-ACL and D / L-ACL, and experimental group 1 using L-ACL as test substance and experimental group 2 using D / L-ACL as test substance.
- Control 1 consisting of only basic feed without test substance and control 2 using L-lysine as test substance were compared together. In each experimental group, the culture was carried out in three repetitions.
- the basic feed and the test substance were mixed in a ratio of 4: 1 (basic feed 0.4 g, test substance 0.1 g, except for control 1, 0.5 g only basic feed), and 0.5 g of the mixed test feed was added to a 125 ml culture bottle. After mixing 50 ml of the prepared anaerobic broth, the mixture was sealed and left in a 39 ° C. incubator to start culture.
- the culture was finally carried out for 72 hours, and culture sampling was conducted at 0h, 48h, 72h after the start of the culture. In order to confirm the culture state, the total gas generation amount of the culture solution sample was primarily measured.
- microbial symbiosis has the ability to decompose and energize the cellulose of plants that cannot be digested by ordinary animals.
- the microbes in the rumen produce a gas, most of which is volatile fatty acids (VFA). Volatile fatty acids are generally generated at 65% acetic acid, 20% propionic acid, and 15% butyric acid, and these volatile fatty acids are used as ruminant energy sources. Journal of Biotechnology Vol. 9 (38), pp. 6229-6232).
- the increase or decrease of volatile fatty acid production by the microorganisms in the rumen can be used as an indicator of the degradation of feed or feed additives, thereby inferring the link between the protective effect of the feed additives in the rumen and the reduction of volatile fatty acid production. have.
- FIG. 3 is a graph showing the rumen bypass rate (%), which compares the relative residual amount (%) of 24h and 48h samples when the residual amount of 0h sample is converted to 100%.
- L-lysine was digested by rumen microorganisms at 0% and 48h after 48h
- L-ACL was digested by rumen microorganisms at 96% at 48h and 90% at 72h. Seemed.
- D / L-ACL showed a rumen bypass rate of 102% at 48h and 91% at 72h. Considering the error range, it can be determined that L-ACL and D / L-ACL are bypassed by almost no degradation by the microorganisms in the rumen until 72h.
- Nutrients not broken down by the microbes in the rumen are absorbed in the small intestine and used for protein synthesis and energy metabolism.
- ACL it is expected that most of it will be delivered to the small intestine due to the high bypass efficiency, and that the amide binding site of the ACL can be converted into lysine form when the amide binding site of the ACL is cleaved by the application of the degrading enzymes present in the small and liver. Absorption will be used.
- the following experiment was conducted.
- cleavage of amide bonds is possible by digestive enzymes such as trypsin and pepsin, and can also be cleaved by hydrolytic enzymes such as asilase.
- digestive enzymes such as trypsin and pepsin
- hydrolytic enzymes such as asilase.
- proteins having peptide bonds by digestive enzymes such as aminopeptidase, carboxypeptidase, endopeptidase, and dipeptidase, which are located in the mucosa
- Digested into amino acids and some dipeptide forms, some undigested dipeptides are also absorbed into cells, hydrolyzed to amino acids by dipeptidase and then transported into the bloodstream. Numerous nutrients in a form that cannot be used directly are absorbed by the small intestine and then moved along the bloodstream to the liver, where they are converted into substances that can be metabolized in the body through various decomposition processes.
- Example 2 One. Small intestine enzyme group
- Example 2 Liver tissue Enzyme group
- liver tissue Mix 0.125g of liver tissue and 1ml of 20mM sodium phosphate buffer (pH7.4) in the liver of Hanwoo (History: KOR005078680400) slaughtered at NACF Bucheon Livestock Products Market, and then glass bead (Sigma G1145) Hepatic tissue was disrupted via beadbeater (MP TM FastPrep®-24) by adding (about 1/10 volume) (20 seconds, 3 times). The hepatic crushing fluid thus obtained was centrifuged at 4 ° C. (14000 rpm, 10 minutes) to secure the supernatant and used in this experiment.
- MP TM FastPrep®-24 beadbeater
- Example 1 in vitro digestion experiments were conducted using enzyme groups of small intestine and liver tissues to determine whether ACLs having a protective function (bypass) in the rumen can be digested and digested in the small intestine.
- the reaction proceeded at a concentration of about 2 g / L ACL, and the exact concentration is specified in the LC quantification results (g / L) of Example 2-4.
- the final volume of the reaction is 1000 ⁇ l and the composition of the substrate, small intestine, and buffer used is specified in Table 4 below.
- Example 2-4 Small intestine enzyme reaction result
- ACL was converted to lysine in the in vitro reaction of small intestine and hepatic enzyme family. From these results, it was confirmed that when the ACL reaches the small intestine through the rumen, it can be converted into lysine by digestive enzymes in the small intestine. The possibility was confirmed. This means that the ACL provided as a feed additive can be utilized directly as lysine in substantially amino acid form in the body of ruminants.
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Abstract
La présente invention concerne un additif alimentaire comprenant un dérivé de lysine, et un procédé d'élevage d'animaux à l'aide de celui-ci.
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KR10-2015-0067661 | 2015-05-14 | ||
KR20150067661 | 2015-05-14 |
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WO (1) | WO2016182403A1 (fr) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0163831B1 (ko) * | 1995-03-18 | 1998-11-16 | 비비바흐, 카르그 | 반추위내의 분해에 대해 보호된 라이신의 제조방법 및 이를 포함한 동물사료 |
JP2003206276A (ja) * | 2002-01-07 | 2003-07-22 | Chisso Corp | α−アミノ−ε−カプロラクタムの製造方法 |
KR20080054732A (ko) * | 2006-12-13 | 2008-06-19 | 대한민국(관리부서:농촌진흥청) | 아미노산이 첨가된 반추위 보호지방의 제조방법 |
KR101167228B1 (ko) * | 2004-06-10 | 2012-07-23 | 보드 오브 트러스티즈 오브 미시건 스테이트 유니버시티 | 라이신으로부터 카프로락탐의 합성 |
KR101288938B1 (ko) * | 2010-12-27 | 2013-07-24 | 김현수 | 캡슐화 코팅된 반추위 보호 아미노산의 제조방법 |
-
2016
- 2016-05-13 WO PCT/KR2016/005118 patent/WO2016182403A1/fr unknown
- 2016-05-13 KR KR1020160058970A patent/KR20160135102A/ko active IP Right Grant
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0163831B1 (ko) * | 1995-03-18 | 1998-11-16 | 비비바흐, 카르그 | 반추위내의 분해에 대해 보호된 라이신의 제조방법 및 이를 포함한 동물사료 |
JP2003206276A (ja) * | 2002-01-07 | 2003-07-22 | Chisso Corp | α−アミノ−ε−カプロラクタムの製造方法 |
KR101167228B1 (ko) * | 2004-06-10 | 2012-07-23 | 보드 오브 트러스티즈 오브 미시건 스테이트 유니버시티 | 라이신으로부터 카프로락탐의 합성 |
KR20080054732A (ko) * | 2006-12-13 | 2008-06-19 | 대한민국(관리부서:농촌진흥청) | 아미노산이 첨가된 반추위 보호지방의 제조방법 |
KR101288938B1 (ko) * | 2010-12-27 | 2013-07-24 | 김현수 | 캡슐화 코팅된 반추위 보호 아미노산의 제조방법 |
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