WO2016158139A1 - 電気的特性測定装置、電気的特性測定方法、血液状態解析システム、及び該方法をコンピューターに実現させるための電気的特性測定用プログラム - Google Patents
電気的特性測定装置、電気的特性測定方法、血液状態解析システム、及び該方法をコンピューターに実現させるための電気的特性測定用プログラム Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
Definitions
- This technology relates to an electrical characteristic measuring device. More specifically, using a blood sample containing a blood cell component and a plasma component, an apparatus for measuring the electrical characteristics of the blood sample, an electrical characteristics measurement method, a blood condition analysis system, and a computer for realizing the method Regarding the program.
- Examples of clinically performed blood state analysis methods include blood coagulation tests and blood ammonia tests.
- a general blood coagulation test prothrombin time (PT), activated partial thromboplastin time (APTT) and the like are known. These methods are methods for analyzing proteins involved in the coagulation reaction contained in plasma obtained by centrifuging a blood sample.
- a general blood ammonia test employs a method of analyzing ammonium ions in plasma using plasma obtained by centrifuging a blood sample, as in the blood coagulation test.
- Patent Document 1 discloses a technique for acquiring information on blood coagulation from the electrical characteristics of a blood sample using electrical characteristics such as a dielectric constant as an index, and “a pair of electrodes and the above pair of electrodes” is disclosed.
- the application means for applying an alternating voltage at predetermined time intervals, the measurement means for measuring the dielectric constant of blood disposed between the pair of electrodes, and the anticoagulant action acting on the blood have been solved.
- a blood coagulation system analyzer having analysis means for analyzing the degree of function of the blood coagulation system using the blood dielectric constant measured at the above time intervals is described.
- the blood sample is not separated into blood components such as plasma components and blood cell components, and whole blood containing all blood components is included. Information on blood coagulation, etc. is obtained using the state.
- the technique for acquiring information related to a blood sample from the electrical characteristics of the blood sample is premised on using a blood sample in a whole blood state.
- a blood sample is previously subjected to centrifugation to obtain a plasma component.
- a lid attached to a container such as a blood collection tube containing a blood sample in the whole blood state is used. It is necessary to open and collect a blood sample for measuring electrical properties. After that, it is necessary to close the lid and use it for the separation process. That is, in this case, it is necessary to provide a recapper device that opens and closes the lid with respect to the container, which increases the manufacturing cost of the device and the running cost of the device.
- the main object of the present technology is to provide a technology capable of acquiring information related to a blood sample from the electrical characteristics of the blood sample even if it is introduced into an existing blood test system.
- an apparatus for measuring the electrical characteristics of a blood sample a mixing unit that mixes the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample
- an electrical property measuring apparatus comprising: a blood sample measuring unit that measures electrical properties of a blood sample mixed by the mixing unit.
- the electrical property measurement apparatus according to the present technology may further include a blood component ratio measurement unit that measures a component ratio of the blood cell component and the plasma component of the blood sample.
- the blood constituent ratio measurement unit of the electrical characteristic measurement apparatus according to the present technology may include an optical detector that detects a boundary surface between the blood cell component and the plasma component.
- the blood constituent ratio measuring unit of the electrical property measuring apparatus may include an electrical detector that detects a boundary surface between the blood cell component and the plasma component.
- the blood sample measuring unit of the electrical property measuring apparatus according to the present technology can also measure a dielectric constant of the blood sample.
- the electrical property measurement apparatus according to the present technology may further include a blood state analysis unit that analyzes the state of the blood sample based on the electrical properties of the blood sample mixed by the mixing unit.
- the electrical property measurement apparatus according to the present technology may further include a correction unit that corrects the measurement result of the electrical property of the blood sample according to the composition ratio of the plasma component.
- the electrical property measurement apparatus according to the present technology may further include a plasma test unit that tests the plasma component.
- the electrical property measurement apparatus according to the present technology may further include a separation unit that separates the plasma component and the blood cell component.
- the present technology is a method for measuring electrical characteristics of a blood sample, the mixing step of mixing the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample, and the mixing And a blood sample measuring step for measuring an electrical property of a blood sample mixed in the process.
- the present technology is further a system for analyzing the state of a blood sample, which is mixed by the mixing unit and the mixing unit that mix the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample.
- An electrical property measuring device comprising a blood sample measuring unit for measuring electrical properties of the blood sample, and blood for analyzing the state of the blood sample based on the electrical properties of the blood sample mixed by the mixing unit
- a blood state analysis system including a blood state analysis device including a state analysis unit is provided.
- the blood state analysis system according to the present technology may also include a server that stores the measurement result by the electrical characteristic measurement device and / or the analysis result by the blood state analysis device.
- the present technology is a program used for measurement of electrical characteristics of a blood sample using a blood sample, and the blood cell component and the plasma component are determined based on a composition ratio of the blood cell component and the plasma component of the blood sample.
- An electrical property measurement program for realizing a mixing function for mixing and a blood sample measurement function for measuring an electrical property of a blood sample mixed by the mixing function is provided.
- the present technology it is possible to introduce into an existing blood test system and acquire information on the blood sample from the electrical characteristics of the blood sample.
- the effect described here is not necessarily limited, and may be any effect described in the present technology.
- FIG. 3 is a drawing-substituting graph showing results by the blood constituent ratio measuring unit shown in FIG.
- FIG. 5 is a drawing-substituting graph showing results by the blood constituent ratio measuring unit shown in FIG. 4.
- FIG. 4 is a schematic conceptual diagram which shows typically the concept of the blood state analysis system which concerns on this technique. It is a flowchart of the electrical property measuring method which concerns on this technique.
- Electrical characteristic measuring device 1 (1) Separation unit 11 (2) Plasma testing unit 12 (3) Mixing unit 13 (4) Blood component ratio measurement unit 14 (5) Blood sample measurement unit 15 (6) Blood state analysis unit 16 (7) Correction unit 17 (8) Storage unit 18 (9) Blood sample Blood state analysis system 10 (1) Electrical characteristic measuring device 1 (2) Blood state analyzer 101 (3) Server 102 (4) Display unit 103 (5) User interface 104 3. Electrical characteristics measurement method (1) Separation process I (2) Plasma testing process II (3) Mixing step III (4) Blood component ratio measurement step IV (5) Blood sample measurement process V (6) Blood state analysis process VI (7) Correction process VII (8) Storage step VIII 4). Program for measuring electrical characteristics
- FIG. 1 is a schematic conceptual diagram schematically showing the concept of an electrical characteristic measuring apparatus 1 (hereinafter also referred to as “apparatus 1”) according to the present technology.
- the electrical characteristic measuring apparatus 1 according to the present technology is an apparatus that measures an electrical characteristic of a blood sample using a blood sample containing a blood cell component and a plasma component (hereinafter also simply referred to as “blood sample”). And at least a mixing unit 13 and a blood sample measurement unit 15.
- storage part 18 etc. can also be provided as needed.
- each part will be described in detail. In the following description, each part will be described in the order of blood test steps that are generally performed, but it is not necessary to have all the parts in the present technology.
- the electrical property measurement apparatus 1 may include a separation unit 11 that separates a blood sample in a whole blood state into a blood cell component and a plasma component as necessary.
- the electrical property measurement apparatus 1 according to the present technology may be configured to use a blood sample that is not provided with the separation unit 11 and is separated into a blood cell component and a plasma component by other means.
- the separation unit 11 is configured to separate a blood sample in a whole blood state into a blood cell component and a plasma component, and the separation method is not particularly limited, and a known separation method can be freely selected and used. For example, a separation method by allowing a blood sample of whole blood to stand for a certain period of time in a blood collection tube containing an anticoagulant, a method of subjecting a blood collection tube containing a blood sample of whole blood to centrifugation, and the like.
- the electrical property measurement apparatus 1 may include a plasma test unit 12 that tests the state of a blood sample using the plasma component separated by the separation unit 11 or another configuration.
- the plasma test unit 12 is not essential, and an external plasma test apparatus can be connected to perform a test using plasma components.
- a known test method using plasma is performed. For example, a method of adding calcium and tissue thromboplastin to plasma and measuring prothrombin time (PT), a method of adding a contact factor activator such as partial thromboplastin and calcium to plasma, and measuring activated partial thromboplastin time (APTT) And a method for examining the ammonia concentration in plasma.
- PT prothrombin time
- APTT activated partial thromboplastin time
- the mixing unit 13 mixes the blood cell component and the plasma component separated by the separation unit 11 or another configuration, and specifically, based on the component ratio of the blood cell component and the plasma component of the blood sample. The blood cell component and the plasma component are mixed.
- the mixing method in the mixing unit 13 is not particularly limited as long as the effects of the present technology are not impaired, and a known mixing method can be freely selected and used. For example, mixing by pipetting, mixing using a mixing rod, etc., mixing by turning the container containing the blood sample upside down, etc. can be mentioned. In the present technology, it is preferable to employ mixing by pipetting from the viewpoint of suppressing scattering of a blood sample and suppressing activation of platelets and the like in the blood sample.
- the ratio of the plasma component is low.
- the hematocrit value differs between the mixed blood sample and the blood sample in the whole blood state before being subjected to the separation step.
- the measurement result is flawed between the electrical characteristics of the blood sample subjected to the separation process and the electrical characteristics of the blood sample not subjected to the separation process.
- a blood sample in a whole blood state before being subjected to the separation step is referred to as a “whole blood sample”.
- the mixing unit 13 includes a dispensing mechanism that dispenses a blood sample according to the component ratio of the separated blood cell component and plasma component.
- the following method is mentioned as an example of the dispensing method based on the component ratio of the blood cell component of the said blood sample, and a plasma component.
- the ratio of the plasma component when the ratio of the plasma component is lower than the ratio of the plasma component in the whole blood sample, a predetermined amount of the blood cell component is aspirated from the liquid phase of the blood cell component using a pipette, and the blood cell component and the plasma component in the whole blood sample The method of making it the same with the composition ratio of is mentioned.
- the ratio of the blood cell component is lower than the ratio of the blood cell component in the whole blood sample, there is a method in which the plasma component is aspirated so that the composition ratio of the plasma component to the blood cell component in the whole blood sample is the same. Can be mentioned.
- a predetermined amount is aspirated from each of the liquid phase of the blood cell component and the liquid phase of the plasma component to newly form a blood sample having the same composition ratio as the blood cell component and the plasma component in the whole blood sample.
- the method of doing is also mentioned.
- the composition ratio of the plasma component to the blood cell component in the mixed blood sample is the same as the composition ratio of the plasma component to the blood cell component in the whole blood sample.
- a method of dispensing the plasma component, the blood cell component, or both components so as to obtain an arbitrary hematocrit value instead of making the composition ratio of the plasma component and the blood cell component in the whole blood sample the same is also included.
- the constituent ratio of the blood cell component and the plasma component in the blood sample subjected to the separation step is the same as that in the whole blood sample, regardless of the constituent ratio.
- a method in which the blood cell component and the plasma component are mixed to obtain whole blood can be employed.
- the blood component ratio measuring unit 14 In the blood component ratio measurement unit 14, the component ratio of the blood cell component and the plasma component in the blood sample is measured before the blood cell component and the plasma component are mixed by the mixing unit 13.
- the blood component ratio measuring unit 14 is not an essential component, but is preferably provided in order to improve the accuracy of analysis.
- the measurement method of the composition ratio in the blood composition ratio measurement unit 14 a known method can be freely selected as long as the effect of the present technology is not impaired. For example, the boundary surface between the separated blood cell component and the plasma component is determined. The method of detecting and measuring the said composition ratio based on this is mentioned.
- FIGS. 2 and 3 Various methods can be freely selected as a method for detecting the boundary surface between the blood cell component and the plasma component.
- an optical measurement method is employed.
- the blood constituent ratio measuring unit 14 includes an optical detector 141 that detects a boundary surface B between the blood cell component S1 and the plasma component S2.
- the optical detector 141 detects the boundary surface B based on the rate at which specific light passes through the blood cell component S1 and the plasma component S2, that is, light transmittance.
- the optical detector 141 includes a light irradiation unit 142 that irradiates the light, and a light detection unit 143 that acquires a voltage signal based on the light emitted from the light irradiation unit 142.
- the light source of the light irradiation unit 142 is not particularly limited as long as it does not affect the physical properties of the blood sample, and may be, for example, a semiconductor laser, that is, a laser diode, a solid laser, or a gas laser. Among these, by using a semiconductor laser, the apparatus can be configured to be small and inexpensive.
- the light detection unit 143 is disposed at a position facing the light irradiation unit 142 across a container T such as a blood collection tube in which a blood sample is stored, and detects light emitted from the light irradiation unit 142.
- the mode of the light detection unit 143 is not particularly limited, and can be set as appropriate according to the type of light emitted from the light irradiation unit 142, and includes a light separation element and a fluorescence detection unit included in a normal light detection unit. Further, a scattered light detection unit or the like may be provided.
- the blood composition ratio measurement unit 14 having the optical detector 141 for example, white light is emitted from the light irradiation unit 142 toward the light detection unit 143.
- the plasma component S2 has a high light transmittance.
- the blood cell component S1 shows a lower light transmittance than the plasma component.
- the blood component ratio measurement unit 14 including such an optical detector 141 is moved up and down with respect to a container T such as a blood collection tube containing a blood sample, as shown in FIG. Before and after the white light passes through the boundary surface B, the light transmittance based on the white light is greatly different, and the boundary surface B can be clearly grasped.
- the boundary surface B is detected using light transmittance as an index.
- the boundary surface B is detected using absorbance or the like as an index. You may do it.
- Another optical measurement method includes a method of irradiating the container T with light, capturing an image that can be grasped by the boundary surface B, and measuring the blood constituent ratio from the captured image. It is done.
- the imaging method is not particularly limited, and a known imaging method can be used.
- a light source array including one or a plurality of light sources is provided in the light irradiation unit 142, and the light detection unit 143 is further provided.
- a method of detecting the boundary surface B by providing a light source array including one or a plurality of light sources.
- an electrical measurement method may be adopted as a method of detecting the boundary surface B.
- an example of the blood composition ratio measurement unit 14 adopting the electrical measurement method will be described with reference to FIGS. 4 and 5.
- the blood constituent ratio measurement unit 14 includes an electric detector 144 that detects a boundary surface B between the blood cell component S1 and the plasma component S2.
- the electric detector 144 has a pipetter and a tip attached to the tip of the pipetter, and the tip is formed of a conductive material.
- the material of the chip is not particularly limited as long as it is a conductive material, and examples thereof include carbon black-containing homopolymer type polypropylene.
- the plasma component is mainly composed of S2, so that the conductivity is high.
- the blood cell component S1 is mainly composed of red blood cells, and exhibits a low conductivity.
- the blood composition ratio measurement unit 14 including the electrical detector 144 may function as the mixing unit 13. That is, when the electric detector 144 is composed of a pipetter and a tip attached to the tip thereof, the blood cell component S1 and the plasma component S2 can be mixed using the pipetter.
- the pipetter and the chip function as the mixing unit 13 and the blood composition ratio measuring unit 14, so that the electrical characteristic measuring device 1 can be downsized and the manufacturing cost of the device 1 can be reduced.
- the boundary surface B is detected using conductivity as an index.
- the boundary surface B is detected using dielectric constant, impedance, admittance, capacitance, conductance, and the like as indexes. You may make it detect.
- Blood sample measurement unit 15 In the blood sample measuring unit 15, the blood cell component S1 and the plasma component S2 are mixed by the mixing unit 13, and the electrical characteristics of the blood sample in the whole blood state are measured.
- examples of electrical properties that can be measured include dielectric constant, impedance, admittance, capacitance, conductance, conductivity, phase angle, and the like. These electrical characteristics can be converted into each other by the mathematical formulas shown in Table 1 below. Therefore, for example, the evaluation result obtained by evaluating the hematocrit value and / or the amount of hemoglobin using the dielectric constant measurement result of the blood sample is the same as the evaluation result obtained when the impedance measurement result of the same blood sample is used.
- the frequency band in which electrical measurement is performed in blood sample measurement unit 15 can be appropriately selected according to the state of the blood sample to be measured, the measurement purpose, and the like.
- the blood sample measuring unit 15 can include one or a plurality of blood sample holding units.
- the blood sample holding unit is not essential.
- the blood sample measuring unit 15 can be designed in a form in which a known cartridge type measurement container or the like can be installed.
- the form of the blood sample holding unit is not particularly limited as long as the blood sample to be measured can be held in the blood sample measuring unit 15, and the blood sample measuring unit 15 has a free form.
- one or a plurality of cells provided on the substrate can function as a blood sample holding unit, or one or a plurality of containers can function as a blood sample holding unit.
- the form is not particularly limited, and a cylindrical body, a polygon having a polygonal cross section (triangle, square or more) can be used as long as the blood sample to be measured can be held.
- a polygon having a polygonal cross section can be used as long as the blood sample to be measured can be held.
- Design freely according to the state of the blood sample and the measurement method such as a cylinder, cone, polygonal cone with a polygonal cross section (triangle, square or more), or a combination of one or more of these. can do.
- the material constituting the container is not particularly limited, and can be freely selected within a range that does not affect the state of the blood sample to be measured and the measurement purpose.
- the type of resin that can be used is not particularly limited, and one or more resins that can be used for holding a blood sample can be freely selected and used.
- hydrophobic and insulating polymers and copolymers such as polypropylene, polymethyl methacrylate, polystyrene, acrylic, polysulfone, polytetrafluoroethylene, and blend polymers may be used.
- the blood sample holding portion with at least one resin selected from polypropylene, polystyrene, acrylic, and polysulfone. This is because these resins have a property of low coagulation activity against blood.
- the blood sample holder is configured to be able to be sealed while holding the blood sample. However, if the time required for measuring the electrical characteristics of the blood sample can be stagnated and the measurement is not affected, the airtight configuration is not necessary.
- the specific introduction and sealing method of the blood sample to the blood sample holding part is not particularly limited, and can be introduced by a free method according to the form of the blood sample holding part.
- a blood sample holder is provided with a lid, and after introducing the blood sample using a pipette or the like, the lid is closed and sealed, or an injection needle is inserted from the outer surface of the blood sample holder, and the blood sample After injecting, a method of sealing the penetrating portion of the injection needle with grease or the like is included.
- the blood sample measurement unit 15 may include one or more application units.
- the application unit is not essential.
- an external application device can be used by designing the blood sample holding unit so that an electrode can be inserted from the outside.
- the application unit starts at a time when a command to start measurement is received or when a power source of the electrical characteristic measuring device 1 is turned on, and a predetermined voltage is applied to the blood sample at each set measurement interval. Apply.
- the number of electrodes used as a part of the application unit and the material constituting the electrodes are not particularly limited as long as the effects of the present technology are not impaired, and any number of electrodes can be configured using a free material.
- any number of electrodes can be configured using a free material.
- titanium, aluminum, stainless steel, platinum, gold, copper, graphite and the like can be mentioned.
- the blood sample measurement unit 15 can also perform a plurality of measurements.
- a method of performing a plurality of measurements for example, a method of performing a plurality of measurements simultaneously by providing a plurality of blood sample measurement units 15, a method of performing a plurality of measurements by scanning one blood sample measurement unit 15, a blood sample Examples thereof include a method of performing a plurality of measurements by moving the holding unit, a method of selecting one or a plurality of blood sample measuring units 15 that are provided with a plurality of blood sample measuring units 15 and actually perform the measurement by switching.
- the blood sample measurement unit 15 preferably has a temperature control function, thereby preventing measurement errors due to the temperature change.
- Blood state analysis unit 16 analyzes the blood state based on the electrical characteristics of the blood sample mixed by the mixing unit 13. This blood state analysis unit 16 is not essential in the electrical characteristic measurement device 1 according to the present technology, and uses an external analysis device or the like based on the measurement result obtained by the electrical property measurement device 1 according to the present technology. It is also possible to analyze the state of the blood sample. If necessary, the test result by the plasma test unit 12 and the measurement result by the blood sample measurement unit 15 may be detected, and the blood state may be analyzed based on the detection result.
- the state of the blood sample that can be analyzed by the blood state analysis unit 16 of the electrical property measuring apparatus 1 according to the present technology is particularly a phenomenon in which a change in the electrical property of the blood sample is observed due to the state change.
- various state changes can be analyzed and evaluated.
- blood coagulation clotting
- fibrin formation fibrin clot formation
- clot formation platelet aggregation
- red blood cell formation blood aggregation
- red blood cell sedimentation red sedimentation
- hemolysis such as clot contraction, fibrinolysis, Fibrinorigis etc.
- correction unit 17 The electrical property measurement apparatus 1 according to the present technology may include a correction unit 17 that corrects the measurement result measured by the blood sample measurement unit 15.
- the correction unit 17 is not essential, and an external measurement result correction device can be connected to correct the measurement result by the blood sample measurement unit 15.
- the electrical characteristic measurement apparatus 1 for example, when a blood cell component and a plasma component are mixed in a state where the plasma component is collected by the plasma test unit 12, the mixed blood sample and the whole blood sample Hematocrit value is different. As a result, a wrinkle occurs in the measurement result between the electrical characteristics of the mixed blood sample and the electrical characteristics of the whole blood sample.
- the correction unit 17 detects a measurement result regarding the component ratio of the blood cell component and the plasma component in the blood sample measured by the blood component ratio measurement unit 14 or the electrical characteristics of the whole blood sample, and the blood sample The measurement result of the electrical characteristics by the measurement unit 15 is corrected. Specifically, correction is performed so that the measurement result of the electrical characteristics by the blood sample measurement unit 15 is equivalent to the measurement result when the electrical characteristics of the whole blood sample are measured.
- the correction method performed in the correction unit 17 is not particularly limited as long as the effect of the present technology is not impaired, and a known correction method can be freely selected and used.
- a known correction method can be freely selected and used.
- the evaluation result of the coagulation ability of the blood sample is greatly influenced by the concentration of the drug at the time of measurement in the plasma component due to the excluded volume effect of the blood cell component in the blood sample.
- the evaluation result of the coagulation ability of a blood sample changes depending on the amount of hemoglobin in the blood sample, even for blood samples collected from the same specimen.
- the correction method includes, for example, a method in which the amount of residual drug or hemoglobin in a blood sample is measured in advance and the measurement result of the electrical characteristics of the blood sample is corrected based on the measured value. It is done.
- the measurement result of the electrical characteristics of the blood sample is corrected by the correction unit.
- the electrical characteristics of the blood sample may be measured.
- the electrical characteristic measurement apparatus 1 includes each analysis result analyzed by the plasma test unit 12, each analysis result analyzed by the blood state analysis unit 16, a measurement result measured by the blood sample measurement unit 15, and the like.
- store can be provided.
- the storage unit 18 is not essential, and each result can be stored by connecting an external storage device.
- the storage unit 18 may be provided separately for each unit, or designed to store various results obtained in each unit in one storage unit 18. It is also possible.
- the blood sample that can be measured is not particularly limited as long as it is a blood sample including at least a blood cell component and a plasma component. You can choose. Specific examples of blood samples include whole blood or diluted solutions thereof, blood samples to which various reagents, anticoagulant release agents, coagulation activators, anticoagulants, platelet activators and antiplatelet agents are added. And so on.
- the electrical characteristic measurement apparatus 1 since the electrical characteristic measurement apparatus 1 according to the present technology as described above includes the mixing unit 13 and the blood sample measurement unit 15, it is an existing blood test system in which a whole blood sample is subjected to a separation process in advance. Even if it is introduced into this, it is possible to acquire information on the blood sample from the electrical characteristics of the blood sample.
- the mixing unit 13 since the mixing unit 13 is provided, there is no need to open and close the lid of a container such as a blood collection tube. The manufacturing cost can be reduced.
- the mixing unit 13 mixes the blood cell component S1 and the plasma component S2 based on the composition ratio of the blood cell component S1 and the plasma component S2 of the blood sample. For example, in dispensing the blood cell component S1 and the plasma component S2 from the blood sample subjected to the separation step, the electrical characteristics of the blood sample subjected to the separation step and the blood sample not subjected to the separation step It is possible to suppress the occurrence of wrinkles in the measurement result as much as possible between the electrical characteristics.
- the mixing unit 13 that mixes the blood cell component and the plasma component based on the composition ratio of the blood cell component S1 and the plasma component S2 of the blood sample is provided.
- a blood sample exhibiting an arbitrary hematocrit value can be newly created.
- the effect described in this specification is an illustration to the last, and is not limited, Any effect described in this technique may be sufficient.
- FIG. 6 is a schematic conceptual diagram schematically showing the concept of the blood state analysis system 10 according to the present technology.
- the blood state analysis system 10 according to the present technology is roughly provided with at least the electrical characteristic measurement device 1 and the blood state analysis device 101.
- the server 102, the display unit 103, the user interface 104, and the like can be provided as necessary. Hereinafter, each part will be described in detail.
- the electrical characteristic measuring apparatus 1 includes at least a mixing unit 13 and a blood sample measuring unit 15. Further, if necessary, a separation unit 11, a plasma test unit 12, a blood composition ratio measurement unit 14, a correction unit 17, and the like can be provided. Since each part with which electrical property measuring device 1 is provided is the same as details of electrical property measuring device 1 mentioned above, explanation is omitted here.
- the blood state analysis apparatus 101 includes a blood state analysis unit 16 that analyzes the state of the blood sample based on the electrical characteristics of the blood sample mixed by the mixing unit 13.
- the blood state analysis unit 16 is the same as the blood state analysis unit 16 of the electrical characteristic measuring apparatus 1 described above, and thus the description thereof is omitted here.
- the server 102 includes a storage unit 18 that stores measurement results obtained by the electrical characteristic measurement device 1 and / or analysis results obtained by the blood state analysis device 101.
- the details of the storage unit 18 are the same as those of the storage unit 18 in the electrical characteristic measuring apparatus 1 described above.
- the display unit 103 displays a measurement result obtained by the electrical characteristic measurement device 1 and / or an analysis result obtained by the blood state analysis device 101. Although a plurality of display units 103 can be provided for each data and result to be displayed, all data and results can be displayed on one display unit 103.
- the user interface 104 is a part for a user to operate. The user can access each part of the blood state analysis system 10 according to the present technology through the user interface 104.
- the electrical characteristic measurement device 1, the blood state analysis device 101, the server 102, the display unit 103, and the user interface 104 may be connected to each other via a network.
- FIG. 7 is a flowchart of an electrical characteristics measuring method according to the present technology.
- the electrical property measurement method according to the present technology is a method for measuring electrical properties of a blood sample, and performs at least a mixing step III and a blood sample measurement step V. Further, if necessary, a separation step I, a plasma test step II, a blood constituent ratio measurement step IV, a blood state analysis step VI, a correction step VII, a storage step VIII and the like can be performed.
- a separation step I, a plasma test step II, a blood constituent ratio measurement step IV, a blood state analysis step VI, a correction step VII, a storage step VIII and the like can be performed.
- each step will be described in detail.
- Separation process I In the separation step I, a blood sample in the whole blood state after blood collection is separated into a blood cell component and a plasma component.
- This separation step I is not an essential step in the electrical property measurement method according to the present technology, and it is possible to separate a blood sample in a whole blood state after blood collection into a blood cell component and a plasma component using another device. It is.
- the details of the separation method performed in the separation step I are the same as the separation method executed by the separation unit 11 of the electrical property measuring apparatus 1 described above. For this reason, explanation is omitted here.
- Plasma testing process II In the plasma test step II, for example, the state of the blood sample is tested using the plasma component separated in the separation step I.
- This plasma test step II is not an essential step for the electrical property measurement method according to the present technology, and it is also possible to test the separated plasma component using an external device or method.
- the details of the testing method performed in the plasma testing process II are the same as the testing method executed by the plasma testing unit 12 of the electrical property measuring apparatus 1 described above. For this reason, explanation is omitted here.
- the mixing step III is a step of mixing the blood cell component and the plasma component separated in the separation step I, for example.
- the details of the mixing method performed in the mixing step III are the same as the mixing method executed in the mixing unit 13 of the electrical property measuring apparatus 1 described above. For this reason, explanation is omitted here.
- Blood component ratio measurement step IV In the blood composition ratio measurement step IV, the composition ratio between the blood cell component and the plasma component in the blood sample is measured before the blood cell component and the plasma component are mixed in the mixing step III.
- This blood composition ratio measurement step IV is not an essential step in the electrical characteristic measurement method according to the present technology, and it is also possible to measure the composition ratio of blood cell components and plasma components using an external device or method. is there.
- the details of the measurement method performed in the blood composition ratio measurement step IV are the same as the measurement method executed by the blood composition ratio measurement unit 14 of the electrical characteristic measurement apparatus 1 described above. For this reason, explanation is omitted here.
- Blood sample measurement process V In the blood sample measurement step V, the blood cell component and the plasma component are mixed in the mixing step III to measure the electrical characteristics of the blood sample that has been brought to the whole blood state.
- the details of the measuring method performed in the blood sample measuring step V are the same as the measuring method executed by the blood sample measuring unit 15 of the electrical property measuring apparatus 1 described above. For this reason, explanation is omitted here.
- Blood state analysis process VI In the blood state analysis step VI, the blood state of the blood sample is analyzed based on the electrical characteristics of the blood sample mixed by the mixing unit 13. This blood state analysis step VI is not an essential step in the electrical property measurement method according to the present technology, and another device or method is used based on the measurement result obtained by the electrical property measurement method according to the present technology. It is also possible to analyze the blood state.
- the details of the analysis method performed in the blood state analysis step VI are the same as the analysis method executed by the blood state analysis unit 16 of the electrical property measuring apparatus 1 described above. For this reason, explanation is omitted here.
- Correction process VII In the correction step VII, the measurement result measured in the blood sample measurement step V is corrected.
- This correction step VII is not an essential step in the electrical characteristic measurement method according to the present technology, and it is also possible to correct the measurement result measured in the blood sample measurement step V using an external device or method. is there.
- the details of the correction method performed in the correction step VII are the same as the correction method executed by the correction unit 17 of the electrical characteristic measuring apparatus 1 described above. For this reason, explanation is omitted here.
- Storage step VIII In the storage step VIII, the measurement result measured in the blood sample measurement step V, each analysis result analyzed in the blood state analysis step VI, the measurement result measured in the blood sample measurement step V, and the like are stored. In the electrical characteristic measuring method according to the present technology, the storing step VIII is not essential, and it is possible to output each time without storing each result. The details of the storage method performed in the storage step VIII are the same as the storage method executed in the storage unit 18 of the electrical characteristic measuring apparatus 1 described above. For this reason, explanation is omitted here.
- the program for measuring electrical characteristics according to the present technology is a program used for measuring electrical characteristics of a blood sample using a blood sample, and the composition ratio of the blood cell component and the plasma component of the blood sample.
- the electrical characteristic measurement program according to the present technology includes a separation function, a plasma test process II, a blood composition ratio measurement process IV, a blood sample measurement process V, a blood condition analysis function, a correction function, and a memory function as necessary. Etc. can also be realized on a computer.
- the electrical characteristic measurement program according to the present technology is a program for causing a computer to realize the above-described electrical property measurement method according to the present technology. Therefore, the details of each function are the same as the respective steps of the above-described electrical characteristic measuring method, and thus description thereof is omitted here.
- this technique can also take the following structures.
- An apparatus for measuring the electrical properties of a blood sample A mixing unit for mixing the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample; A blood sample measurement unit for measuring electrical characteristics of the blood sample mixed by the mixing unit; An electrical characteristic measuring device comprising: (2) The electrical characteristic measuring device according to (1), further comprising a blood constituent ratio measuring unit that measures a constituent ratio of the blood cell component and the plasma component of the blood sample. (3) The electrical component measurement device according to (2), wherein the blood constituent ratio measurement unit includes an optical detector that detects an interface between the blood cell component and the plasma component.
- the electrical component measurement device includes an electrical detector that detects an interface between the blood cell component and the plasma component.
- the electrical property measurement device measures a dielectric constant of the blood sample.
- the electrical property measuring apparatus according to any one of (1) to (5), further comprising a blood state analyzing unit that analyzes a state of the blood sample based on an electrical property of the blood sample mixed by the mixing unit. .
- the electrical property measuring apparatus according to any one of (1) to (6), further including a correction unit that corrects the measurement result of the electrical property of the blood sample according to the composition ratio of the plasma component.
- a method for measuring the electrical properties of a blood sample comprising: A mixing step of mixing the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample; And a blood sample measuring step of measuring an electrical property of the blood sample mixed in the mixing step.
- a system for analyzing the state of a blood sample An electric device comprising: a mixing unit that mixes the blood cell component and the plasma component based on a composition ratio of the blood cell component and the plasma component of the blood sample; and a blood sample measurement unit that measures an electrical characteristic of the blood sample mixed by the mixing unit Characteristic measuring device; A blood state analysis device comprising a blood state analysis unit for analyzing the state of the blood sample based on the electrical characteristics of the blood sample mixed by the mixing unit; A blood state analysis system. (12) The blood state analysis system according to (11), further comprising a server that stores measurement results obtained by the electrical characteristic measurement device and / or analysis results obtained by the blood state analysis device.
- a program used for measuring electrical characteristics of a blood sample using the blood sample A mixing function for mixing the blood cell component and the plasma component based on the composition ratio of the blood cell component and the plasma component of the blood sample, and a blood sample measurement function for measuring electrical characteristics of the blood sample mixed by the mixing function; Program for measuring electrical characteristics to realize
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Abstract
Description
また、一般的な血中アンモニア検査も、前記血液凝固検査と同様、血液試料を遠心分離して得られる血漿を用いて、当該血漿中のアンモニウムイオンを分析する方法を採用している。
このため、臨床的に行われる血液状態の解析方法を行う検査機関や病院などの医療機関に導入されている血液検査システムでは、患者等から採取された血液試料を速やかに遠心分離する工程が採用されている。
その一方で、医療機関等に導入されている血液検査システムでは、前述の如く、予め血液試料を遠心分離に供し、血漿成分を得ることを前提している。
本技術に係る電気的特性測定装置は、前記血液試料の前記血球成分と前記血漿成分の構成比を測定する血液構成比測定部をさらに備えることもできる。
本技術に係る電気的特性測定装置の前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する光学検出器を有することもできる。
本技術に係る電気的特性測定装置の前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する電気検出器を有することもできる。
本技術に係る電気的特性測定装置の前記血液試料測定部は、前記血液試料の誘電率を測定することもできる。
本技術に係る電気的特性測定装置は、前記混合部により混合された血液試料の電気的特性に基づいて、該血液試料の状態を解析する血液状態解析部を更に備えることもできる。
本技術に係る電気的特性測定装置は、前記血漿成分の構成比に応じて、前記血液試料の電気的特性の測定結果を補正する補正部を更に備えることもできる。
本技術に係る電気的特性測定装置は、前記血漿成分を検査する血漿検査部を更に備えることもできる。
本技術に係る電気的特性測定装置は、前記血漿成分と血球成分とを分離する分離部を更に備えることもできる。
本技術に係る血液状態解析システムは、前記電気的特性測定装置による測定結果及び/または血液状態解析装置による解析結果を記憶するサーバーを備えることもできる。
なお、ここに記載された効果は、必ずしも限定されるものではなく、本技術中に記載されたいずれかの効果であってもよい。
1.電気的特性測定装置1
(1)分離部11
(2)血漿検査部12
(3)混合部13
(4)血液構成比測定部14
(5)血液試料測定部15
(6)血液状態解析部16
(7)補正部17
(8)記憶部18
(9)血液試料
2.血液状態解析システム10
(1)電気的特性測定装置1
(2)血液状態解析装置101
(3)サーバー102
(4)表示部103
(5)ユーザーインターフェース104
3.電気的特性測定方法
(1)分離工程I
(2)血漿検査工程II
(3)混合工程III
(4)血液構成比測定工程IV
(5)血液試料測定工程V
(6)血液状態解析工程VI
(7)補正工程VII
(8)記憶工程VIII
4.電気的特性測定用プログラム
図1は、本技術に係る電気的特性測定装置1(以下、「装置1」ともいう)の概念を模式的に示す模式概念図である。本技術に係る電気的特性測定装置1は、血球成分と血漿成分とを含む血液試料(以下、単に「血液試料」ともいう)を用いて、該血液試料の電気的特性を測定する装置であって、混合部13及び血液試料測定部15を少なくとも備える。また、必要に応じて、分離部11、血漿検査部12、血液構成比測定部14、血液状態解析部16、補正部17、記憶部18などを備えることもできる。以下、各部について詳細に説明する。尚、以下では、概ね通常行われる血液検査の工程順に即して各部の説明を行うが、本技術において全ての部を備えている必要はない。
前記血球成分と血漿成分を含む血液試料を用いた種々の血液検査には、血漿成分を用いるものがある。このため、血液検査を行う場合、予め前記血液試料を血球成分と血漿成分とに分離することが通常行われている。
本技術に係る電気的特性測定装置1は、必要に応じて、全血状態の血液試料を血球成分と血漿成分とに分離する分離部11を備えていてもよい。その一方で、本技術に係る電気的特性測定装置1は、分離部11を備えず、他の手段により血球成分と血漿成分に分離された血液試料を用いる構成としてもよい。
本技術に係る電気的特性測定装置1には、前記分離部11又は他の構成により分離された血漿成分を用いて、血液試料の状態を検査する血漿検査部12を備えることができる。本技術に係る電気的特性測定装置1において、血漿検査部12は必須ではなく、外部の血漿検査装置を接続して、血漿成分を用いた検査を行うことも可能である。
前記混合部13は、前記分離部11又は他の構成により分離された血球成分と血漿成分とを混合するものであり、具体的には、血液試料の血球成分と血漿成分の構成比に基づいて、前記血球成分と血漿成分との混合が行われる。
前記血液試料の血球成分と血漿成分の構成比に基づく分注方法の一例としては、下記方法が挙げられる。
又は、ピペットを用いて、血球成分の液相及び血漿成分の液相それぞれから所定量吸引し、全血試料における血球成分と血漿成分の構成比と同一の構成比からなる血液試料を新たに形成する方法も挙げられる。
前記血液構成比測定部14では、前記混合部13により血球成分と血漿成分とが混合される前に、血液試料における血球成分と血漿成分との構成比が測定される。本技術に係る電気的特性測定装置1において、この血液構成比測定部14は必須の構成ではないが、解析の精度を高めるためには備えていることが好ましい。
前記光検出部143は、血液試料が収容された採血管等の容器Tを挟んで前記光照射部142と対向する位置に配置され、当該光照射部142から照射された光を検出する。この光検出部143の態様は特に限定されず、前記光照射部142から照射される光の種類に応じて適宜設定することができ、通常の光検出手段が備える、光分離素子、蛍光検出部、散乱光検出部などを備えていてもよい。
ここで、血液試料において、血漿成分はS2主に水分から構成されていることから、電導率が高い値を示す。一方で、血球成分S1は主に赤血球から構成され、電導率が低い値を示す。
ここで、本技術に係る電気的特性測定装置1では、前記電気検出器144を有する血液構成比測定部14が混合部13として機能する構成としてもよい。すなわち、前記電気検出器144がピペッターとその先端に装着するチップからなる構成である場合、このピペッターを用いて血球成分S1と血漿成分S2とを混合することも可能である。この場合、ピペッター及びチップは、混合部13と血液構成比測定部14として機能することになり、電気的特性測定装置1の小型化や当該装置1の製造コストの削減を実現することができる。
血液試料測定部15では、前記混合部13により血球成分S1と血漿成分S2とが混合され、全血状態とされた血液試料の電気的特性が測定される。
本技術に係る電気的特性測定装置1において、測定可能な電気的特性としては、例えば、誘電率、インピーダンス、アドミッタンス、キャパシタンス、コンダクタンス、導電率、位相角などを挙げることができる。これらの電気的特性は、下記表1に示す数式によって、互いに変換可能である。そのため、例えば、血液試料の誘電率測定の結果を用いてヘマトクリット値及び/又はヘモグロビン量を評価した評価結果は、同一の血液試料のインピーダンス測定の結果を用いた場合の評価結果と同一になる。これらの電気量や物性値の多くは複素数を用いて記述することができ、それによって変換式を簡略化することができる。また、血液試料測定部15において、電気的測定を行う周波数帯域は、測定する血液試料の状態、測定目的などに応じて、適宜選択することができる。
血液状態解析部16では、前記混合部13により混合された血液試料の電気的特性に基づいて、血液状態の解析が行われる。この血液状態解析部16は、本技術に係る電気的特性測定装置1では必須ではなく、本技術に係る電気的特性測定装置1で得られた測定結果に基づいて、外部の解析装置等を用いて血液試料の状態を解析することも可能である。また、必要に応じて、前記血漿検査部12による検査結果、血液試料測定部15による測定結果などを検出し、それに基づいて血液状態の解析が行われるようにしてもよい。
本技術に係る電気的特性測定装置1は、前記血液試料測定部15で測定された測定結果を補正する補正部17を備えることができる。本技術に係る電気的特性測定装置1において、補正部17は必須ではなく、外部の測定結果補正装置を接続して、血液試料測定部15による測定結果を補正することも可能である。
ここで、血液試料の凝固能の評価結果は、測定時における薬剤が、血液試料中の血球成分の排除体積効果によって血漿成分中に濃縮されることにより、大きな影響を受けることが知られている。更に、血液試料の凝固能の評価結果は、同一検体から採取された血液試料であっても、当該血液試料におけるヘモグロビン量に応じて変化することが知られている。このため、前記補正方法としては、例えば、血液試料中の薬剤残余量やヘモグロビン量を予め測定しておき、その測定値に基づいて血液試料の電気的特性の測定結果を補正する方法などが挙げられる。
尚、本技術では、前記補正部により血液試料の電気的特性の測定結果を補正しているが、例えば、検査時に使用される薬剤の添加量を制御することにより、補正することが必要ないよう、血液試料の電気的特性を測定するようにしてもよい。
本技術に係る電気的特性測定装置1は、血漿検査部12で解析された各解析結果、血液状態解析部16で解析された各解析結果、血液試料測定部15で測定された測定結果などを記憶する記憶部18を備えることができる。本技術に係る電気的特性測定装置1において、記憶部18は必須ではなく、外部の記憶装置を接続して、各結果を記憶することも可能である。
本技術に係る電気的特性測定装置1において、測定対象とすることが可能な血液試料は、少なくとも血球成分と血漿成分とを含む血液試料であれば特に限定されず、自由に選択することができる。血液試料の具体例としては、全血又はこれの希釈液、各種試薬や抗凝固処理解除剤、凝固活性化剤、抗凝固剤、血小板活性化剤及び抗血小板剤等の薬剤を添加した血液試料などを挙げることができる。
しかしこの場合、リキャッパー装置を導入する必要があり、その分装置の製造コストの増加や当該装置のランニングコストが嵩んでしまう。
更に、本技術に係る電気的特性測定装置1によれば、血液試料の血球成分S1と血漿成分S2の構成比に基づき、前記血球成分と前記血漿成分を混合する混合部13を備えているため、任意のヘマトクリット値を示す血液試料を新たに作成することができる。その結果、ヘマトクリット値の影響を考慮せずに、血液試料の状態、特に凝固能を精度よく解析することができる。
なお、本明細書に記載された効果はあくまで例示であって限定されるものではなく、本技術中に記載されたいずれかの効果であってもよい。
図6は、本技術に係る血液状態解析システム10の概念を模式的に示す模式概念図である。本技術に係る血液状態解析システム10は、大別して、電気的特性測定装置1と、血液状態解析装置101と、を少なくとも備える。また、必要に応じて、サーバー102、表示部103、ユーザーインターフェース104などを備えることもできる。以下、各部について詳細に説明する。
電気的特性測定装置1は、混合部13、血液試料測定部15を少なくとも備える。また、必要に応じて、分離部11、血漿検査部12、血液構成比測定部14、補正部17などを備えることもできる。なお、電気的特性測定装置1が備える各部は、前述した電気的特性測定装置1の詳細と同一であるため、ここでは説明を割愛する。
血液状態解析装置101は、前記混合部13により混合された血液試料の電気的特性に基づいて、血液試料の状態を解析する血液状態解析部16を備える。なお、血液状態解析部16は、前述した電気的特性測定装置1の血液状態解析部16と同一であるため、ここでは説明を割愛する。
サーバー102には、電気的特性測定装置1での測定結果及び/又は血液状態解析装置101での解析結果を記憶する記憶部18を備える。記憶部18の詳細は、前述した電気的特性測定装置1における記憶部18と同一である。
表示部103では、電気的特性測定装置1での測定結果及び/又は血液状態解析装置101での解析結果などが表示される。表示部103は、表示するデータや結果毎に、複数設けることも可能であるが、一つの表示部103に、全てのデータや結果を表示することも可能である。
ユーザーインターフェース104は、ユーザーが操作するための部位である。ユーザーは、ユーザーインターフェース104を通じて、本技術に係る血液状態解析システム10の各部にアクセスすることができる。
図7は、本技術に係る電気的特性測定方法のフローチャートである。本技術に係る電気的特性測定方法は、血液試料の電気的特性を測定する方法であって、少なくとも混合工程III、血液試料測定工程Vを行う。また、必要に応じて、分離工程I、血漿検査工程II、血液構成比測定工程IV、血液状態解析工程VI、補正工程VII、記憶工程VIIIなどを行うこともできる。以下、各工程について詳細に説明する。
分離工程Iでは、採血後の全血状態の血液試料を血球成分と血漿成分とに分離を行う。この分離工程Iは、本技術に係る電気的特性測定方法では必須の工程ではなく、別の装置を用いて採血後の全血状態の血液試料を血球成分と血漿成分とに分離することも可能である。分離工程Iで行う分離方法の詳細は、前述した電気的特性測定装置1の分離部11で実行される分離方法と同一である。このため、ここでは説明を割愛する。
血漿検査工程IIでは、例えば分離工程Iにより分離された血漿成分を用いて血液試料の状態の検査を行う。この血漿検査工程IIは、本技術に係る電気的特性測定方法に必須の工程でなく、外部の装置や方法を用いて、分離された血漿成分の検査を行うことも可能である。血漿検査工程IIで行う検査方法の詳細は、前述した電気的特性測定装置1の血漿検査部12で実行される検査方法と同一である。このため、ここでは説明を割愛する。
混合工程IIIは、例えば分離工程Iにより分離された血球成分と血漿成分とを混合する工程である。混合工程IIIで行う混合方法の詳細は、前述した電気的特性測定装置1の混合部13で実行される混合方法と同一である。このため、ここでは説明を割愛する。
血液構成比測定工程IVでは、前記混合工程IIIにより血球成分と血漿成分とが混合される前に、血液試料における血球成分と血漿成分との構成比を測定する。この血液構成比測定工程IVは、本技術に係る電気的特性測定方法に必須の工程でなく、外部の装置や方法を用いて、血球成分と血漿成分との構成比を測定することも可能である。
血液構成比測定工程IVで行う測定方法の詳細は、前述した電気的特性測定装置1の血液構成比測定部14で実行される測定方法と同一である。このため、ここでは説明を割愛する。
血液試料測定工程Vでは、前記混合工程IIIにより血球成分と血漿成分とが混合され、全血状態とされた血液試料の電気的特性を測定する。
血液試料測定工程Vで行う測定方法の詳細は、前述した電気的特性測定装置1の血液試料測定部15で実行される測定方法と同一である。このため、ここでは説明を割愛する。
血液状態解析工程VIでは、前記混合部13により混合された血液試料の電気的特性に基づいて、当該血液試料の血液状態の解析が行われる。この血液状態解析工程VIは、本技術に係る電気的特性測定方法では必須の工程ではなく、本技術に係る電気的特性測定方法で得られた測定結果に基づいて、別の装置や方法を用いて血液状態の解析を行うことも可能である。血液状態解析工程VIで行う解析方法の詳細は、前述した電気的特性測定装置1の血液状態解析部16で実行される解析方法と同一である。このため、ここでは説明を割愛する。
補正工程VIIでは、前記血液試料測定工程Vで測定された測定結果を補正する。この補正工程VIIは、本技術に係る電気的特性測定方法に必須の工程でなく、外部の装置や方法を用いて、前記血液試料測定工程Vで測定された測定結果を補正することも可能である。補正工程VIIで行う補正方法の詳細は、前述した電気的特性測定装置1の補正部17で実行される補正方法と同一である。このため、ここでは説明を割愛する。
記憶工程VIIIでは、血液試料測定工程Vで測定された測定結果や、血液状態解析工程VIで解析された各解析結果、血液試料測定工程Vで測定された測定結果などを記憶する。本技術に係る電気的特性測定方法において、記憶工程VIIIは必須ではなく、各結果を記憶せずに、その都度、アウトプットすることも可能である。記憶工程VIIIで行う記憶方法の詳細は、前述した電気的特性測定装置1の記憶部18で実行される記憶方法と同一である。このため、ここでは説明を割愛する。
本技術に係る電気的特性測定用プログラムは、血液試料を用いた該血液試料の電気的特性の測定に用いるプログラムであって、血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合機能と、前記混合機能により混合された血液試料の電気的特性を測定する血液試料測定機能と、を実現させるための電気的特性測定用プログラムである。
また、本技術に係る電気的特性測定用プログラムは、必要に応じて、分離機能、血漿検査工程II、血液構成比測定工程IV、血液試料測定工程V、血液状態解析機能、補正機能、記憶機能などをコンピューターに実現させることも可能である。
(1)
血液試料の電気的特性を測定する装置であって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合部と、
前記混合部により混合された血液試料の電気的特性を測定する血液試料測定部と、
を備える電気的特性測定装置。
(2)
前記血液試料の前記血球成分と前記血漿成分の構成比を測定する血液構成比測定部をさらに備える(1)に記載の電気的特性測定装置。
(3)
前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する光学検出器を有する(2)記載の電気的特性測定装置。
(4)
前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する電気検出器を有する(2)記載の電気的特性測定装置。
(5)
前記血液試料測定部は、前記血液試料の誘電率を測定する(1)から(4)のいずれかに記載の電気的特性測定装置。
(6)
前記混合部により混合された血液試料の電気的特性に基づいて、該血液試料の状態を解析する血液状態解析部を更に備える(1)から(5)のいずれかに記載の電気的特性測定装置。
(7)
前記血漿成分の構成比に応じて、前記血液試料の電気的特性の測定結果を補正する補正部を更に備える(1)から(6)のいずれかに記載の電気的特性測定装置。
(8)
前記血漿成分を検査する血漿検査部を更に備える(1)から(7)のいずれかに記載の電気的特性測定装置。
(9)
前記血漿成分と血球成分とを分離する分離部を更に備える(1)から(8)のいずれかに記載の電気的特性測定装置。
(10)
血液試料の電気的特性を測定する方法であって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合工程と、
前記混合工程により混合された血液試料の電気的特性を測定する血液試料測定工程と、を行う電気的特性測定方法。
(11)
血液試料の状態を解析するシステムであって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合部及び前記混合部により混合された血液試料の電気的特性を測定する血液試料測定部を備える電気的特性測定装置と、
前記混合部により混合された血液試料の電気的特性に基づいて、該血液試料の状態を解析する血液状態解析部を備える血液状態解析装置と、
を有する血液状態解析システム。
(12)
前記電気的特性測定装置による測定結果及び/または血液状態解析装置による解析結果を記憶するサーバーを備える(11)記載の血液状態解析システム。
(13)
血液試料を用いた該血液試料の電気的特性の測定に用いるプログラムであって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合機能と、前記混合機能により混合された血液試料の電気的特性を測定する血液試料測定機能と、を実現させるための電気的特性測定用プログラム。
11 分離部
12 血漿検査部
13 混合部
14 血液構成比測定部
15 血液試料測定部
16 血液状態解析部
17 補正部
18 記憶部
10 血液状態解析システム
101 血液状態解析装置
102 サーバー
103 表示部
104 ユーザーインターフェース
141 光学検出器
142 光照射部
143 光検出部
144 電気検出器
I 分離工程
II 血漿検査工程
III 混合工程
IV 血液構成比測定工程
V 血液試料測定工程
VI 血液状態解析工程
VII 補正工程
VIII 記憶工程
Claims (13)
- 血液試料の電気的特性を測定する装置であって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合部と、
前記混合部により混合された血液試料の電気的特性を測定する血液試料測定部と、
を備える電気的特性測定装置。 - 前記血液試料の前記血球成分と前記血漿成分の構成比を測定する血液構成比測定部をさらに備える請求項1に記載の電気的特性測定装置。
- 前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する光学検出器を有する請求項2記載の電気的特性測定装置。
- 前記血液構成比測定部は、前記血球成分と前記血漿成分の境界面を検出する電気検出器を有する請求項2記載の電気的特性測定装置。
- 前記血液試料測定部は、前記血液試料の誘電率を測定する請求項1に記載の電気的特性測定装置。
- 前記混合部により混合された血液試料の電気的特性に基づいて、該血液試料の状態を解析する血液状態解析部を更に備える請求項1記載の電気的特性測定装置。
- 前記血漿成分の構成比に応じて、前記血液試料の電気的特性の測定結果を補正する補正部を更に備える請求項1記載の電気的特性測定装置。
- 前記血漿成分を検査する血漿検査部を更に備える請求項1記載の電気的特性測定装置。
- 前記血漿成分と血球成分とを分離する分離部を更に備える請求項1記載の電気的特性測定装置。
- 血液試料の電気的特性を測定する方法であって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合工程と、
前記混合工程により混合された血液試料の電気的特性を測定する血液試料測定工程と、を行う電気的特性測定方法。 - 血液試料の状態を解析するシステムであって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合部及び前記混合部により混合された血液試料の電気的特性を測定する血液試料測定部を備える電気的特性測定装置と、
前記混合部により混合された血液試料の電気的特性に基づいて、該血液試料の状態を解析する血液状態解析部を備える血液状態解析装置と、
を有する血液状態解析システム。 - 前記電気的特性測定装置による測定結果及び/または血液状態解析装置による解析結果を記憶するサーバーを備える請求項11記載の血液状態解析システム。
- 血液試料を用いた該血液試料の電気的特性の測定に用いるプログラムであって、
血液試料の血球成分と血漿成分の構成比に基づき、前記血球成分と前記血漿成分を混合する混合機能と、前記混合機能により混合された血液試料の電気的特性を測定する血液試料測定機能と、を実現させるための電気的特性測定用プログラム。
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- 2016-02-29 WO PCT/JP2016/055974 patent/WO2016158139A1/ja active Application Filing
- 2016-02-29 JP JP2017509408A patent/JP6729558B2/ja active Active
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Also Published As
Publication number | Publication date |
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EP3279649A4 (en) | 2018-12-26 |
EP3279649A1 (en) | 2018-02-07 |
JP6729558B2 (ja) | 2020-07-22 |
JPWO2016158139A1 (ja) | 2018-01-25 |
US10634660B2 (en) | 2020-04-28 |
CN107407650B (zh) | 2020-09-11 |
CN107407650A (zh) | 2017-11-28 |
US20180080920A1 (en) | 2018-03-22 |
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