WO2016153263A3 - Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells - Google Patents

Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells Download PDF

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Publication number
WO2016153263A3
WO2016153263A3 PCT/KR2016/002914 KR2016002914W WO2016153263A3 WO 2016153263 A3 WO2016153263 A3 WO 2016153263A3 KR 2016002914 W KR2016002914 W KR 2016002914W WO 2016153263 A3 WO2016153263 A3 WO 2016153263A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
tonsil
derived stem
spheroid form
differentiation
Prior art date
Application number
PCT/KR2016/002914
Other languages
French (fr)
Korean (ko)
Other versions
WO2016153263A2 (en
Inventor
조인호
박윤신
이상훈
황지영
전예슬
진윤미
김경아
김하영
김한수
정성철
Original Assignee
이화여자대학교 산학협력단
고려대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 이화여자대학교 산학협력단, 고려대학교 산학협력단 filed Critical 이화여자대학교 산학협력단
Priority to US15/561,500 priority Critical patent/US10870830B2/en
Priority claimed from KR1020160034484A external-priority patent/KR101885122B1/en
Publication of WO2016153263A2 publication Critical patent/WO2016153263A2/en
Publication of WO2016153263A3 publication Critical patent/WO2016153263A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/55—Glands not provided for in groups A61K35/22 - A61K35/545, e.g. thyroids, parathyroids or pineal glands
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to: a method for producing a spheroid form of tonsil-derived stem cells, the method enhancing growth and differentiation efficiency of tonsil-derived stem cells; and a method for producing a spheroid form of parathyroid hormones from the same. The method of the present invention for producing a spheroid form of tonsil-derived stem cells cultures the tonsil-derived stem cells in the form of a spheroid to thereby enhance the proliferation rate of the stem cells per se and significantly increase differentiation potency into parathyroid cells. Thereby, the method has a remarkable effect on the growth and differentiation of the tonsil-derived stem cells into parathyroid cells, etc.
PCT/KR2016/002914 2015-03-26 2016-03-23 Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells WO2016153263A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/561,500 US10870830B2 (en) 2015-03-26 2016-03-23 Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20150042774 2015-03-26
KR10-2015-0042774 2015-03-26
KR10-2016-0034484 2016-03-23
KR1020160034484A KR101885122B1 (en) 2015-03-26 2016-03-23 Spheroidal culture method of tonsil-derived mesenchymal stem cells for accelerating differentiation efficacy and maintenance of stemness

Publications (2)

Publication Number Publication Date
WO2016153263A2 WO2016153263A2 (en) 2016-09-29
WO2016153263A3 true WO2016153263A3 (en) 2017-01-05

Family

ID=56977835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/002914 WO2016153263A2 (en) 2015-03-26 2016-03-23 Method for culturing differentiation-promoting and -sustaining spheroid form of tonsil-derived stem cells

Country Status (1)

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WO (1) WO2016153263A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101017710B1 (en) * 2009-02-05 2011-02-25 한양대학교 산학협력단 Adult stem cell population of cell spheroid form for transplantation and method for producing the same
KR101282926B1 (en) * 2011-07-28 2013-07-08 고려대학교 산학협력단 Surface tension induced concave microwell fabrication and cell aggregation using the same
KR20140113139A (en) * 2013-03-15 2014-09-24 고려대학교 산학협력단 Cell spheroid culture plate
KR20140126626A (en) * 2013-04-23 2014-10-31 이화여자대학교 산학협력단 Method of producing parathyroid hormone from tonsil-derived mesenchymal stem cell

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101017710B1 (en) * 2009-02-05 2011-02-25 한양대학교 산학협력단 Adult stem cell population of cell spheroid form for transplantation and method for producing the same
KR101282926B1 (en) * 2011-07-28 2013-07-08 고려대학교 산학협력단 Surface tension induced concave microwell fabrication and cell aggregation using the same
KR20140113139A (en) * 2013-03-15 2014-09-24 고려대학교 산학협력단 Cell spheroid culture plate
KR20140126626A (en) * 2013-04-23 2014-10-31 이화여자대학교 산학협력단 Method of producing parathyroid hormone from tonsil-derived mesenchymal stem cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
INGRAM, PATRICK ET AL.: "Spheroid Cell Culture on PDMS Hydrophobic Surfaces and Integration into Microfluidic Devices", 15TH INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES, 6 October 2011 (2011-10-06), pages 1539 - 1541, XP055343544 *

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Publication number Publication date
WO2016153263A2 (en) 2016-09-29

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