WO2015199976A8 - Target activated microdissection - Google Patents

Target activated microdissection Download PDF

Info

Publication number
WO2015199976A8
WO2015199976A8 PCT/US2015/034930 US2015034930W WO2015199976A8 WO 2015199976 A8 WO2015199976 A8 WO 2015199976A8 US 2015034930 W US2015034930 W US 2015034930W WO 2015199976 A8 WO2015199976 A8 WO 2015199976A8
Authority
WO
WIPO (PCT)
Prior art keywords
biological sample
transfer film
target
selectively
reagent
Prior art date
Application number
PCT/US2015/034930
Other languages
French (fr)
Other versions
WO2015199976A1 (en
Inventor
Robert F. Bonner
Nicole Y. Morgan
Thomas J. Pohida
Philip G. MCQUEEN
Randall H. PURSLEY
John KAKAREKA
Original Assignee
The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The United States Of America, As Represented By The Secretary, Department Of Health And Human Services filed Critical The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Publication of WO2015199976A1 publication Critical patent/WO2015199976A1/en
Publication of WO2015199976A8 publication Critical patent/WO2015199976A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method of removing a target from a biological sample, comprising: providing a substrate-mounted biological sample with a reagent that selectively acts on the target within the biological sample, wherein the reagent comprises an agent that absorbs light of a wavelength that selectively heats the reagent; placing a flexible transfer film having a lower surface and an opposing upper surface such that the lower surface of the transfer film is adjacent to the biological sample; applying a uniform air pressure gradient across the flexible transfer film to maintain thermal contact between the lower surface of the transfer film and the biological sample; exposing the biological sample to the light of the wavelength to selectively heat the reagent and produce a change in the transfer film resulting in selectively adhering the target to the transfer film; and selectively removing the target from the biological sample by removing the transfer film with the adhered target from the biological sample.
PCT/US2015/034930 2014-06-24 2015-06-09 Target activated microdissection WO2015199976A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462016571P 2014-06-24 2014-06-24
US62/016,571 2014-06-24

Publications (2)

Publication Number Publication Date
WO2015199976A1 WO2015199976A1 (en) 2015-12-30
WO2015199976A8 true WO2015199976A8 (en) 2016-02-25

Family

ID=53484166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/034930 WO2015199976A1 (en) 2014-06-24 2015-06-09 Target activated microdissection

Country Status (1)

Country Link
WO (1) WO2015199976A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106053165A (en) * 2016-05-16 2016-10-26 常州大学 Novel optical method for observing microscopic structure of mucoprotein of sputum
JP2021535403A (en) * 2018-08-07 2021-12-16 エックスエムディー ダイアグノスティクス，インコーポレイテッド Equipment and cartridges for extracting target biological sample regions and molecules
SG11202105311VA (en) * 2018-11-29 2021-06-29 Univ La Trobe Method of identifying a structure
DE102020119764A1 (en) 2020-07-27 2022-01-27 Rheinisch-Westfälische Technische Hochschule Aachen (RWTH), Körperschaft des öffentlichen Rechts Device and method for the preparation and preparation of tissue samples

Family Cites Families (25)

* Cited by examiner, † Cited by third party
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US4444487A (en) 1979-07-02 1984-04-24 Xerox Corporation Multiple-flash fuser
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4745055A (en) 1985-05-07 1988-05-17 California Biotechnology Inc. Fused protein for enzyme immunoassay system
CA1340456C (en) 1986-07-07 1999-03-23 Hubert J.P. Schoemaker Chimeric rodent/human immunoglobulins specific for tumor-associated antigens
GB8626412D0 (en) 1986-11-05 1986-12-03 Clark M R Antibodies
US4856351A (en) * 1987-10-27 1989-08-15 Amoco Corporation Sample chamber and system for analyzing fluid inclusions
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
ATE138106T1 (en) 1988-07-20 1996-06-15 David Segev METHOD FOR AMPLIFICATION AND DETECTION OF NUCLEIC ACID SEQUENCES
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
KR950703809A (en) 1993-07-14 1995-09-20 에프. 제이. 스미트 Circuit arrangement for charging rechargeable batteries
US5843644A (en) 1994-03-01 1998-12-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Isolation of cellular material under microscopic visualization using an adhesive/extraction reagent tipped probe
US5843657A (en) 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
AU711115B2 (en) 1994-07-15 1999-10-07 Akzo Nobel N.V. Use of RNA polymerase to improve nucleic acid amplification process
US5883144A (en) 1994-09-19 1999-03-16 Sentinel Products Corp. Silane-grafted materials for solid and foam applications
AT402203B (en) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr METHOD FOR TRANSCRIPTION-FREE AMPLIFICATION OF NUCLEIC ACIDS
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5859699A (en) 1997-02-07 1999-01-12 Arcturus Engineering, Inc. Laser capture microdissection analysis vessel
US6469779B2 (en) 1997-02-07 2002-10-22 Arcturus Engineering, Inc. Laser capture microdissection method and apparatus
ES2230877T3 (en) 1998-07-30 2005-05-01 The Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services MICRODISECTION OF PRECISION FOR CAPTURE WITH LASER USING SHORT PULSE LENGTH.
EP1153300A2 (en) 1999-02-16 2001-11-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Lcm (laser capture microdissection) for cellular protein analysis
US20030215936A1 (en) 2000-12-13 2003-11-20 Olli Kallioniemi High-throughput tissue microarray technology and applications
US7695752B2 (en) * 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
WO2012097058A1 (en) * 2011-01-12 2012-07-19 1St Detect Corporation Evacuating a sample chamber

Also Published As

Publication number Publication date
WO2015199976A1 (en) 2015-12-30

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