WO2015199342A1 - Bactericidal and pesticidal agent composition containing plant extract as active ingredient - Google Patents
Bactericidal and pesticidal agent composition containing plant extract as active ingredient Download PDFInfo
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- WO2015199342A1 WO2015199342A1 PCT/KR2015/005509 KR2015005509W WO2015199342A1 WO 2015199342 A1 WO2015199342 A1 WO 2015199342A1 KR 2015005509 W KR2015005509 W KR 2015005509W WO 2015199342 A1 WO2015199342 A1 WO 2015199342A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Definitions
- Corresponding components include carbofuran, dichlorvos, methidathion, methomyl, cadusafos, edifenphos, monocrotophos, azinphos-methyl, tefluthrin, cyfluthrin, zeta-cypermethrin, methiocarb, blasticidin-s, and beta-cyfluthrin.
- These chemical constituents cause environmental disturbances such as the reduction of soil microorganisms and natural enemies, as well as water and soil pollution and pesticide residues from agricultural products.
- 2007-0031979 discloses a main ingredient of natural insecticides such as oleander, cheonnyeonja, baekbugeun, cypress seeds, juniper tree, baekryechae (jihwangyeon) , Natural pesticides of crops of plant components mixed with extracts of acidic seaweed are disclosed in a specific ratio, and Korean Patent Publication No. 2009-0057591 discloses plant seeds having bactericidal properties such as apricot, peach, ginkgo, gilyeong and sancho as steam.
- An object of the present invention is to provide a bactericidal insecticide composition exhibiting excellent bactericidal and insecticidal effects on a wide range of plants. It aims to provide.
- the bactericidal insecticide composition of the present invention aims at minimizing the weakness of the fruit trees and the like, as well as removing soil contamination or residual pesticides.
- an object of the present invention is to provide an antimicrobial pesticide composition
- a plant extract excellent in antimicrobial activity excellent in the control effect such as anthrax, fungi, powdery mildew, etc. commonly occurring in all crops.
- the present invention provides a bactericidal and insecticide composition in which distilled water is put into a ginkgo bark ripened for 6 months with oleander, pore hole, garlic and baekduong, and the sulfur reactant is mixed with an extract extracted from hot water.
- oleander is a plant belonging to the oleander family, which is native to India, is native to Jeju Island and southern provinces in Korea, and grows to about 2-4 m.
- the leaves are narrow, the stem is like bamboo, and the flower color is peach-like, and it is called oleander ( ⁇ ⁇ ⁇ ) .
- the leaf is also willow-like, and the flower color is called reddish red. Because it is colorful in color or white, it is planted not only for landscaping but also for ornamental use, but racine, digitoxin, gitoxin and oleandrin are contained in leaves, roots and stems. It is very toxic and its power is known to be 6,000 times that of cyanide (potassium cyanide; KCN).
- Zhagong is a perennial plant, also called Jangok, belonging to the Pygmyaceae or Phytolaccae. It grows well in all around bushes, forests, roadsides, valleys, wastelands, fertile pastures, and glades. ) And seat hole (landing) and island seat ball grow.
- American Pong Gong contains 4-5% of phytolactoxin (C 24 H 30 O 8 ), saponins, potassium acetate, etc., and these ingredients are known to be effective in controlling silver mites and aphid oil. Allicin, an active ingredient of garlic, has been shown to be antibacterial and bactericidal.
- It contains sitosterol, hederagenin, oleanolic acid alc and a small amount of acetyloleanolic acid and a very small amount of acetyloleanolic acid. It has antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Ginkgo bark has a pest dislike odor, which acts as a pest repellent, and the various ingredients contained in the product stick to the leaves or stems of the crop for a long time, thus maintaining its long-term efficacy.
- the plant extract containing sulphate, turmeric, garlic, baekduong, and sulfur reactant in which sulfur is dissolved in caustic soda in hot water extracted extract by adding distilled water to the matured ginkgo bark as an active ingredient Bactericidal and insecticide compositions.
- the present invention relates to a bactericidal and insecticidal composition
- a plant extract as an active ingredient
- the composition of the present invention is excellent in bactericidal and insecticidal and antimicrobial activity against pathogens of various plants, in particular excellent in controlling the nematode of ginseng It is an extract of a plant that does not use chemical pesticides at all, and removes problems such as soil and environmental pollution at the same time, and no harmful components of pesticides remaining in the crops are detected, thereby eliminating harmful controversy caused by chemical pesticides. There is an effect such as improving the quality.
- the present invention is characterized in that it provides a bactericidal and insecticide composition comprising as an active ingredient a plant extract mixed with a solution in which sulfur is dissolved in caustic soda as an active ingredient.
- 10 to 30 parts by weight of perforated porcelain, 30 to 50 parts by weight of garlic, 30 to 50 parts by weight of Baekduong and 30 to 50 parts by weight of garlic and 100 to 200 parts by weight of aged ginkgo shells 700 to 1000 parts by weight of distilled water is put in a thermostat maintaining 60 to 90 °C, and extracted with hot water for 6 to 10 days.
- the oleander may use leaves or stems and roots, but usually uses pruned stems and leaves. After removing the foreign substances on the leaves or stems of the oleander, and then crushed by using a crusher, dried for 2-3 days in the shade without sunlight, perforated, garlic and pasqueflower roots are put in a blender and crushed.
- Ginkgo bark is placed in a container and aged for 6 months while maintaining a constant temperature of 20 -30 °C.
- S Sulfur (S) 2-4Kg and caustic soda (NaOH) is added 1.5-2 Kg and stirred for 0.5 to 1 hour with a rubber rod to prepare a sulfur reactant.
- Equation 1 The reaction formula between sulfur and caustic soda is shown in Equation 1.
- the reaction is exothermic, so the temperature of the reaction rises to 90-100 °C should be careful.
- the liquid composition for sterilizing insecticides of the present invention containing the extract and the sulfur reactant can be obtained.
- Aged ginkgo shell was prepared in the same manner as in Example 1 except that 1.5Kg was used.
- Transparent band size is less than 2 mm
- Example 1 of the present invention was found to be superior to Example 3 in controlling the nematode.
- the extract prepared in Example 1 was antibacterial against soft rot, Downy mildew, Black spot, Black root rot, and phytophthora rot using agar diffusion method. The spectrum was measured.
- the bactericidal insecticide composition of the present invention not only minimizes the weakness of the fruit trees and the like, but also has a function of removing soil contamination or residual pesticides, and controlling anthrax, downy mildew, powdery mildew, etc. commonly occurring in all crops. It is beneficial as an antimicrobial pesticide composition comprising a plant extract having excellent antimicrobial activity as an effective ingredient, and is particularly useful as an environmentally friendly pesticide by effectively controlling nematodes generated in ginseng roots.
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Abstract
The present invention relates to a bactericidal and pesticidal composition containing a plant extract as an active ingredient. The composition of the present invention has excellent bactericidal, pesticidal, and antibacterial activities against pathogens of various plants; fundamentally removes problems, such as soil or environmental pollution, by using a plant extract with an excellent effect of preventing, especially, ginseng nematode, entirely without agricultural chemicals; and improves the harvest or quality of crops while calming controversy about harmfulness by agricultural chemicals due to the non-detection of harmful components of the agricultural chemicals remaining on the crops. Specifically, the present invention is characterized by providing a bactericidal and pesticidal agent composition containing, as an active ingredient, a plant extract obtained by mixing a sulfur reaction material with an extract, the extract being prepared by placing Nerium indicum, Phytolacca esculenta, garlic, Pulsatilla koreana Nakai, and aged ginkgo skins, together with distilled water, in a constant-temperature bath, followed by aging at 60-90°C for 6-10 days.
Description
본 발명은 천연 식물인 협죽도, 자리공, 마늘 및 백두옹((白頭翁, 할미꽃뿌리)과 6개월 숙성시킨 은행 껍질에 증류수을 첨가하여 열수 추출한 추출액에 유황을 가성소다에 반응시킨 반응물을 적정비율로 혼합한 식물추출물을 유효성분으로 포함하는 살균 및 살충제 조성물에 관한 발명이다.The present invention is a plant in which the reaction mixture of sulfur reacted with caustic soda in an appropriate ratio is added to distilled water by adding distilled water to ginkgo biloba which is a natural plant, oleander, perilla, garlic, and baekduong ((할 頭 翁)). The present invention relates to a bactericidal and insecticide composition comprising the extract as an active ingredient.
병해충 등에 의한 농작물의 생장 발육을 저하로 결실물의 수확량이 낮거나 품질이 떨어지는 등의 문제점이 있어 현대 농업에서는 화학 살충제 등의 농약 사용이 필요불가결한 요인으로 등장하게 되었으나, 과거 수십년간 화학농약을 사용한 관계로 병해충들은 내성이 생겨 시간이 지날수록 더 많은 양의 농약을 사용하거나 또는 보다 치명적인 맹독성의 농약을 사용하여야만 방제효과를 얻을 수 있는 부작용을 낳고 있다. 각종 농작물의 성장을 저해하는 해충이나 세균들의 방제를 위해 국내에서 출하된 농약의 세계보건기구 독성분류에 의한 맹독성에 해당하는 성분들을 살펴보면 phosphamidon, terbufos, phorate, EPN, ethoprophos, tebupirimfos 등이 있고, 고독성에 해당하는 성분들은 carbofuran, dichlorvos, methidathion, methomyl, cadusafos, edifenphos, monocrotophos, azinphos-methyl, tefluthrin, cyfluthrin, zeta-cypermethrin, methiocarb, blasticidin-s, beta-cyfluthrin 등이 있다. 이들 화학 성분들에 의해 토양 미생물 및 천적이 감소하는 등의 생태계 교란은 물론 수질 및 토양 오염과 농산물의 농약잔류 등으로 환경 및 인체상의 유해 문제가 사회적 이슈로 부각되어 친환경 농업을 실현하기 위한 저독성 또는 무독성, 무공해성의 천연농약 또는 생물 농약에 대한 연구가 활발히 진행되고 있다.Due to problems such as low yield and poor quality of crops due to deterioration of growth of crops due to pests, etc., in modern agriculture, the use of chemical pesticides and other pesticides has become an indispensable factor. As a result, pests become resistant and have adverse effects that can only be achieved by using more pesticides or more lethal pesticides over time. In order to control pests and bacteria that inhibit the growth of various crops, the components corresponding to the toxic toxicity by the WHO classification of pesticides shipped from Korea are phosphamidon, terbufos, phorate, EPN, ethoprophos, tebupirimfos, etc. Corresponding components include carbofuran, dichlorvos, methidathion, methomyl, cadusafos, edifenphos, monocrotophos, azinphos-methyl, tefluthrin, cyfluthrin, zeta-cypermethrin, methiocarb, blasticidin-s, and beta-cyfluthrin. These chemical constituents cause environmental disturbances such as the reduction of soil microorganisms and natural enemies, as well as water and soil pollution and pesticide residues from agricultural products. There is an active research on non-toxic, pollution-free natural or biological pesticides.
그 일례로 한국등록특허 제10-1079491호에서는 과수 병해 방제용 살균제로 살균성 식물 추출물로서 차나무 또는 울금 추출물을, 살균성 식물 정유로서 피마자오일(Castor oil), 클로브 버드(Clove bud), 타임(Thyme) 등을 포함하는 살균제에 관한 기술이 개시되어 있고, 한국공개특허 제2007-0031979호에는 천연 살충제의 주원료인 고두자, 천련자, 백부근, 측백나무종자, 주엽나무, 백굴채(지황련), 명아주, 산해박의 추출물을 특정비율로 혼합한 식물성분의 농작물 천연 살충제가 개시되어 있으며, 한국공개특허 제2009-0057591호에는 살구, 복숭아, 은행, 길경, 산초 등 살균 능력이 있는 식물씨앗을 증기로 쪄서 추출한 목초액과 나노화된 황, 실리카 및 아미노산, 미네랄, 휴믹산을 키토산과 혼합시켜 제조한 용액이 이용한 무독성 살균제에 관한 기술이 개시되어 있다. 한국등록특허 제10-0478146호에는 " 협죽도과 식물의 잎 추출물을 함유하는 고려잔디갈색퍼짐병 방제용 조성물 및 이를 이용하여 갈색퍼짐병을방제하는 방법에 관한 기술이 개시되어 있다. 이를 구체적으로 살펴보면, 갈색퍼짐병(Large patch)은 조이시아 속(Zoysia sp.) 잔디에 발생하는 병으로 이 병을 일으키는 병원균은 리족토니아 솔라니(Rhizoctonia solani) AG2-2(IV)으로 협죽도과에 속하는 협죽도 식물체의 잎 추출물이 고려잔디의 갈색퍼짐병(Large patch)을 방제하는데 우수한 효과를 나타냄을 발견하였다는 내용이 기술되어 있다. 그러나 협죽도, 자리공, 마늘, 백두옹 및 숙성시킨 은행 껍질을 열수 추출한 추출액과 유황 반응물을 혼합한 혼합액이 과수류나, 엽채 과채류, 근채류, 곡류 및 특용작물 등의 해충이나 선충 등을 효율적으로 방제하는 효과가 있다는 사실은 개시된 바 없다.For example, Korean Patent No. 10-1079491 discloses a tea or turmeric extract as a bactericidal plant extract as a fungicide for controlling fruit diseases, and castor oil, a clove bud and a thyme as a bactericidal plant essential oil. A technique related to fungicides including the like is disclosed, and Korean Laid-Open Patent Publication No. 2007-0031979 discloses a main ingredient of natural insecticides such as oleander, cheonnyeonja, baekbugeun, cypress seeds, juniper tree, baekryechae (jihwangyeon) , Natural pesticides of crops of plant components mixed with extracts of acidic seaweed are disclosed in a specific ratio, and Korean Patent Publication No. 2009-0057591 discloses plant seeds having bactericidal properties such as apricot, peach, ginkgo, gilyeong and sancho as steam. Disclosed is a non-toxic fungicide used by a solution prepared by mixing vinegar extracted with steam and nanoized sulfur, silica, amino acids, minerals, and humic acid with chitosan. Korean Patent No. 10-0478146 discloses "a composition for controlling Korea grass brown spread disease containing leaf extracts of oleander plants and a method for controlling brown spread disease using the same. Large patch is a disease that occurs in the grass of Zoysia sp. The pathogen causing the disease is Rhizoctonia solani AG2-2 (IV). It has been described that the extract has been found to have an excellent effect on the control of large patches of Goryeo grass, but the extracts and sulfur reactants were extracted from hot water extracts of oleanders, pore holes, garlic, baekduong and aged ginkgo husks. The mixed solution can effectively control pests and nematodes such as fruit trees, leafy vegetables, root vegetables, cereals and special crops. No bar actually disclosed.
본 발명은 광범위한 식물에 대해 우수한 살균 및 살충 효과를 나타내는 살균 살충제 조성물을 제공하는 것을 목적으로 하며, 과수류나 엽채 과채류, 근채류, 곡류 및 특용작물 등의 해충이나 선충을 효율적으로 방제하는 살균 살충제 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a bactericidal insecticide composition exhibiting excellent bactericidal and insecticidal effects on a wide range of plants. It aims to provide.
본 발명의 살균 살충제 조성물은 상기 과수류 등의 약해를 최소화할 뿐만 아니라, 토양오염이나 잔류 농약 등을 원천적 제거함을 목적으로 한다.The bactericidal insecticide composition of the present invention aims at minimizing the weakness of the fruit trees and the like, as well as removing soil contamination or residual pesticides.
또한, 본 발명은 모든 작물에 공통적으로 발생하는 탄저병, 노균병, 흰가루병 등의 방제효과가 뛰어난 항균 활성이 우수한 식물 추출물을 유효성분으로 포함하는 항균용 농약 조성물을 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide an antimicrobial pesticide composition comprising a plant extract excellent in antimicrobial activity excellent in the control effect such as anthrax, fungi, powdery mildew, etc. commonly occurring in all crops.
특히, 본 발명은 인삼뿌리에 발생하는 선충을 효율적으로 방제하는 살균 살충제 조성물을 제공하는 것을 목적으로 한다.In particular, it is an object of the present invention to provide a bactericidal insecticide composition for effectively controlling nematodes generated in ginseng roots.
상기와 같은 본 발명의 목적을 달성하기 위하여 본 발명은 협죽도, 자리공, 마늘, 백두옹과 6개월간 숙성시킨 은행 껍질에 증류수를 넣고 열수 추출한 추출액에 유황반응물을 혼합한 살균 및 살충제 조성물을 제공한다.In order to achieve the object of the present invention as described above, the present invention provides a bactericidal and insecticide composition in which distilled water is put into a ginkgo bark ripened for 6 months with oleander, pore hole, garlic and baekduong, and the sulfur reactant is mixed with an extract extracted from hot water.
본 발명의 목적은 과수류나 엽채 과채류, 근채류, 곡류 및 특용작물 등의 해충이나 선충, 병원균을 효율적으로 방제하기 위한 친환경적인 살균 살충제 조성물로 특정 종류의 병해에 한정하는 것은 아니지만, 실험을 통해 바람직하게는 인삼선충, 탄저병(anthracnose), 무름병(Soft rot), 노균병(Downy mildew), 검은무늬병(Black spot), 검은뿌리 썩음병(Black root rot), 역병( Phytophthora rot), 노균병(Downy mildew) 및 흰루병((Pyllactinia kakicola) 등에 다양하게 적용될 수 있다.An object of the present invention is an environmentally friendly bactericidal insecticide composition for efficiently controlling pests, nematodes and pathogens such as fruit trees, leafy vegetables, root vegetables, cereals and special crops, but is not limited to specific types of diseases, but preferably through experiments. Ginseng nematodes, anthracnose, soft rot, Downy mildew, Black spot, Black root rot, Phytophthora rot, Downy mildew and It can be variously applied to white fever disease (Pyllactinia kakicola).
본 발명의 조성성분 중 협죽도는 협죽도과에 속하는 식물로 인도가 원산지로, 한국에서는 제주도 및 남부 지방에 자생하고 있으며, 약 2-4m 정도까지 자란다. 잎이 좁고, 줄기는 대나무 같고, 꽃 색깔이 복숭아 같다하여 협죽도(狹竹挑)라고 불리우며,또한 그 잎이 버들잎 같고, 꽃색깔이 봉숭아처름 붉다하여 유도화 (柳桃花)라고도 하며 꽃의 색깔은 붉은색 또는 흰색으로 화려하여 조경수는 물론 관상용으로도 심고 있으나, 라신(racine), 디기톡신(digitoxin), 기톡신(gitoxin) 및 올레안드린(Oleandrin,) 등이 잎이나 뿌리, 줄기 등에 다량함유되어 있어 매우 독성이 강하며 그 위력은 청산가리(시안화 칼륨 ; KCN)의 6천 배라고 알려져 있다. Among the components of the present invention, oleander is a plant belonging to the oleander family, which is native to India, is native to Jeju Island and southern provinces in Korea, and grows to about 2-4 m. The leaves are narrow, the stem is like bamboo, and the flower color is peach-like, and it is called oleander (잎 竹 挑) .The leaf is also willow-like, and the flower color is called reddish red. Because it is colorful in color or white, it is planted not only for landscaping but also for ornamental use, but racine, digitoxin, gitoxin and oleandrin are contained in leaves, roots and stems. It is very toxic and its power is known to be 6,000 times that of cyanide (potassium cyanide; KCN).
자리공은 자리공과 또는 상륙과(Phytolaccae)에 속하는 장록이라고도 불리우는 다년생 식물로 사방이 트인 수풀, 숲 주변, 길가, 산골짜기, 황무지, 비옥한 목초지, 빈터 등에 잘 자라며, 우리나라에는 미국자리공(붉은대자리공)과 자리공(상륙) 및 섬자리공이 자라고 있다. 미국자리공에는 4~5%의 피톨락카톡신(C24 H30 08), 사포닌, 초산칼륨 등이 함유되어 있어 이들 성분을은 응애류, 진딧물유 등의 방제에 효과가 뛰어난 것으로 알려져 있다. 마늘의 유효성분인 알리신은 항균 및 살균작용을 하는 것으로 밝혀졌다. 마늘은 Escherichia, Samonella, Staphylococcus, Streptococcus, Klebsiella, Gram 양성균 및 Gram 음성균에 대한 광범위한 항균작용을 나타낸다. 상기 알리신의 항균작용에 관여하는 주된 메커니즘은 thiolsulfinates의 신속한 작용으로 인해 미생물의 특정 티올 함유효소를 저해하기 때문인 것으로 추정하고 있다. 백두옹(白頭翁)은 미나리아재비과에 속하는 다년생 초본식물로 할미꽃이라고도 불리우며 4-5월에 꽃이 피고, 우리나라 전국의 산과 들의 양지쪽에서 흔히 볼 수 있는 식물로 그 뿌리에는 stigmasterol(C29 H46 O)과 β-sitosterol이 함유되어 있고 또 hederagenin, oleanolic acid alc 극소량의 acetyloleanolic acid 및 극소량의 acetyloleanolic acid도 함유되어 있어 고초균(枯草菌)과 황색포도상 구균에 대하여 항균작용으로 가지고 있다. 은행 껍질은 해충이 싫어하는 냄새를 함유하고 있어서 해충 기피제의 역할을 하며 또한 제품에 함유된 다양한 성분들이 농작물의 잎이나 줄기에 오랫동안 달라붙어서 약효를 오래동안 지속시키는 기능을 한다.Zhagong is a perennial plant, also called Jangok, belonging to the Pygmyaceae or Phytolaccae. It grows well in all around bushes, forests, roadsides, valleys, wastelands, fertile pastures, and glades. ) And seat hole (landing) and island seat ball grow. American Pong Gong contains 4-5% of phytolactoxin (C 24 H 30 O 8 ), saponins, potassium acetate, etc., and these ingredients are known to be effective in controlling silver mites and aphid oil. Allicin, an active ingredient of garlic, has been shown to be antibacterial and bactericidal. Garlic has a wide range of antibacterial activity against Escherichia, Samonella, Staphylococcus, Streptococcus, Klebsiella, Gram positive bacteria and Gram negative bacteria. It is presumed that the main mechanism involved in the antimicrobial action of allicin is that the rapid action of thiolsulfinates inhibits specific thiol-containing enzymes of microorganisms. Baekduong (白頭翁) is a perennial herbaceous plant belonging to the Buttercupaceae family, also called Pasqueflower, which blooms in April-May, and is commonly found on the hills of the mountains and fields of Korea. It contains sitosterol, hederagenin, oleanolic acid alc and a small amount of acetyloleanolic acid and a very small amount of acetyloleanolic acid. It has antibacterial activity against Bacillus subtilis and Staphylococcus aureus. Ginkgo bark has a pest dislike odor, which acts as a pest repellent, and the various ingredients contained in the product stick to the leaves or stems of the crop for a long time, thus maintaining its long-term efficacy.
본 발명의 실시예에서 알 수 있듯이 협죽도, 자리공, 마늘, 백두옹 및 숙성시킨 은행 껍질에 증류수를 첨가하여 열수 추출한 추출액에 유황을 가성소다에 용해시킨 유황 반응물을 첨가한 식물추출물을 유효성분으로 포함하는 살균 및 살충제 조성물이다.As can be seen in the embodiment of the present invention, the plant extract containing sulphate, turmeric, garlic, baekduong, and sulfur reactant in which sulfur is dissolved in caustic soda in hot water extracted extract by adding distilled water to the matured ginkgo bark as an active ingredient. Bactericidal and insecticide compositions.
본 발명은 식물 추출물을 유효성분으로 포함하는 살균 및 살충 조성물에 관한 것으로 본 발명의 상기 조성물은 각종 식물의 병원균에 대하여 살균 및 살충과 항균 활성이 우수하며, 특히 인삼의 선충을 방제하는 효과가 뛰어난 화학 농약을 전혀 사용하지 않은 식물의 추출물로 토양이나 환경의 오염 등의 문제점을 원천적으로 제거함과 동시에 농작물에 잔류하는 농약의 유해 성분이 검출되지 않아 화학 농약에 의한 유해 논란을 불식시키면서도 농작물의 수확이나 품질을 높이는 등의 효과가 있다.The present invention relates to a bactericidal and insecticidal composition comprising a plant extract as an active ingredient, the composition of the present invention is excellent in bactericidal and insecticidal and antimicrobial activity against pathogens of various plants, in particular excellent in controlling the nematode of ginseng It is an extract of a plant that does not use chemical pesticides at all, and removes problems such as soil and environmental pollution at the same time, and no harmful components of pesticides remaining in the crops are detected, thereby eliminating harmful controversy caused by chemical pesticides. There is an effect such as improving the quality.
본 발명은 협죽도, 자리공, 마늘, 할미꽃 뿌리 및 은행 껍질을 숙성시킨 추출물에 유황을 가성소다에 용해시킨 용액을 혼합한 식물추출물을 유효성분으로 포함하는 살균 및 살충제 조성물을 제공함을 특징으로 한다.The present invention is characterized in that it provides a bactericidal and insecticide composition comprising as an active ingredient a plant extract mixed with a solution in which sulfur is dissolved in caustic soda as an active ingredient.
보다 구체적으로 설명하면, 협죽도 100중량부에 대하여 자리공 10 내지 30중량부, 마늘 30 내지 50중량부, 백두옹 30 내지 50중량부 및 마늘 30 내지 50중량부와 숙성시킨 은행껍질 100 내지 200중량부에 증류수 700 내지 1000중량부를 넣고 60 내지 90℃를 유지하는 항온기에 넣고, 6 내지 10일간 열수 추출시킨다. To explain more specifically, 10 to 30 parts by weight of perforated porcelain, 30 to 50 parts by weight of garlic, 30 to 50 parts by weight of Baekduong and 30 to 50 parts by weight of garlic and 100 to 200 parts by weight of aged ginkgo shells 700 to 1000 parts by weight of distilled water is put in a thermostat maintaining 60 to 90 ℃, and extracted with hot water for 6 to 10 days.
상기의 협죽도는 잎이나 줄기 및 뿌리를 사용할 수 있으나, 통상적으로 가지치기를 한 줄기 및 잎을 사용한다. 상기의 협죽도 잎이나 줄기에 묻어있는 이물질을 제거한 후 파쇄기를 이용하여 파쇄한 후 햇빛이 들지 않는 그늘에서 2 - 3일간 건조시키고, 자리공, 마늘 및 할미꽃 뿌리는 믹서기 등에 넣어 분쇄한다. The oleander may use leaves or stems and roots, but usually uses pruned stems and leaves. After removing the foreign substances on the leaves or stems of the oleander, and then crushed by using a crusher, dried for 2-3 days in the shade without sunlight, perforated, garlic and pasqueflower roots are put in a blender and crushed.
은행껍질은 용기에 넣고 20 -30℃ 일정하게 온도를 유지하면서 6개월간 숙성시킨다. 상기의 숙성이 끝난 은행껍질과 파쇄한 협죽도, 자리공, 마늘, 백두옹을 증류수와 함께 항온기에 넣고 60 내지 90℃의 온도를 유지하면서 7 - 10 일간 숙성시킨 후 체로 걸러 추출액을 얻는다. Ginkgo bark is placed in a container and aged for 6 months while maintaining a constant temperature of 20 -30 ℃. The aging ginkgo bark and crushed oleander, peregrine, garlic, baekduong with distilled water and put in a constant temperature for 60 to 90 ℃ while maintaining a temperature of 7 to 10 days after sieving to obtain an extract.
유황(S) 2 - 4Kg과 가성소다 (NaOH)을 1.5 - 2 Kg을 넣고 0.5 내지 1시간 동안 고무 막대 등으로 저어 주면 유황반응물을 제조한다.Sulfur (S) 2-4Kg and caustic soda (NaOH) is added 1.5-2 Kg and stirred for 0.5 to 1 hour with a rubber rod to prepare a sulfur reactant.
상기의 유황과 가성소다간의 반응식은 식 1과 같다 The reaction formula between sulfur and caustic soda is shown in Equation 1.
[식 1][Equation 1]
4S + 6NaOH → 2Na2S + Na2S2O3+ 3H2O4S + 6NaOH → 2Na 2 S + Na 2 S 2 O 3 + 3H 2 O
상기의 반응은 발열반응으로 반응시 온도가 90 - 100℃까지 상승하므로 주의를 요하여야 한다. The reaction is exothermic, so the temperature of the reaction rises to 90-100 ℃ should be careful.
상기의 추출액 10ℓ에 식 1의 반응물 3 내지 5 Kg을 넣고 0.5 내지 1시간 동안 고무 막대 등으로 저어 주면 추출물과 유황 반응물을 함유하는 본 발명의 살균 살충용 액상 조성물을 얻을 수 있다.When the reaction solution 3 to 5 Kg of the formula 1 is added to 10 l of the extract and stirred with a rubber rod for 0.5 to 1 hour, the liquid composition for sterilizing insecticides of the present invention containing the extract and the sulfur reactant can be obtained.
이하 실시예를 통하여 본 발명의 살균 살충용 액상 조성물에 대하여 더욱 상세히 설명한다. 본 실시예는 본 발명의 바람직한 일 예시를 설명할 뿐, 본 발명의 기술적 사상이 이에 한정되는 것은 아니다.Hereinafter will be described in more detail with respect to the sterilizing insecticidal liquid composition of the present invention. The present embodiment only describes a preferred example of the present invention, but the technical idea of the present invention is not limited thereto.
[실시예 1]Example 1
이물질을 제거한 가지치기한 협죽도 1Kg을 파쇄하여 그늘에서 48시간 건조시킨다. 자리공 200g과 마늘 200g, 백두옹 200g을 믹서기에 넣고, 분쇄한다. 6개월간 숙성시킨 은행 껍질 2Kg과 상기의 파쇄물 및 분쇄물을 하부에 스크린이 설치된 항온기에 증류수 11ℓ과 함께 넣고 혼합한다. 상기 항온기의 온도를 80℃로 유지하면서 8일간 숙성시킨다. 숙성이 끝나면 상기의 파쇄물 및 분쇄물로부터 추출액을 분리한 후 상기의 추출액 10ℓ에 상기 식 1의 유황 반응물 3.5Kg을 넣고 고무막대로 1시간 동안 교반한 후 8시간 방치한다. 방치가 끝난 상기의 추출액을 포장용기에 넣고 포장한다.Pruned oleander, which has been removed from foreign matters, is crushed and dried in the shade for 48 hours. 200 g of perforated, 200 g of garlic and 200 g of Baekduong are put in a blender and ground. 2Kg of ginkgo skin aged for 6 months and the above crushed matter and ground product are mixed together with 11 liters of distilled water in a thermostat equipped with a screen at the bottom. It is aged for 8 days while maintaining the temperature of the thermostat at 80 ℃. After aging, the extract is separated from the crushed matter and the pulverized product, and 3.5 kg of the sulfur reactant of Formula 1 is added to 10 l of the extract, stirred for 1 hour with a rubber rod, and left for 8 hours. Put the above extracted liquid into a packaging container and package it.
[실시예 2]Example 2
숙성시킨 은행껍질을 1.5Kg을 사용하는 것을 제외하고는 실시예 1과 동일하게 제조하였다.Aged ginkgo shell was prepared in the same manner as in Example 1 except that 1.5Kg was used.
[실시예3]Example 3
추출액 10ℓ에 유황 반응물 3Kg을 사용한 것을 제외하고는 실시예 1과 동일하게 제조하였다.It was prepared in the same manner as in Example 1 except that 3Kg of sulfur reactant was used in 10 L of the extract.
본 발명의 실시예에 따른 살충, 살균 조성물의 주요 성분은 식물의 추출물로 화학 농약의 문제점인 내성이 발생하지 않으며, EPA(Environmental Protection Agency, 미국 환경 보호청) 규제 물질이 포함되어 있지 않고, 농약 성분이 없기 때문에 잔류 농약도 검출되지 않는다. 본 발명의 실시예에 따른 식물 추출물을 이용한 살균 살충제에 대하여 화학 농약이 사용된 식물에서 주로 검출되는 잔류 농약의 유해 성분 검출 여부를 검사하였다. 검사결과, Acephate, Acetamiprid, Bendiocarb, Bitertanol, Diazinon, Ethylenebis, Parathion, Simazine, Tebupirimfos, Zoxamide 등 240개 항목의 성분이 모두 불검출되었다.The main component of the insecticidal and bactericidal composition according to the embodiment of the present invention is a plant extract, which does not generate resistance which is a problem of chemical pesticides, and does not contain EPA (Environmental Protection Agency, US Environmental Protection Agency) controlled substance, Since there is no residual pesticide is detected. The fungicide insecticide using the plant extract according to an embodiment of the present invention was tested for the detection of harmful components of residual pesticides mainly detected in plants using chemical pesticides. As a result, 240 items including Acephate, Acetamiprid, Bendiocarb, Bitertanol, Diazinon, Ethylenebis, Parathion, Simazine, Tebupirimfos and Zoxamide were not detected.
[실험예 1]Experimental Example 1
1. 인삼 선충에 대한 향균 효과1. Antibacterial Effects on Ginseng Nematodes
실시예 1 내지 3에서 수득한 추출물을 사용하여 인삼 선충에 대한 항균효과를 측정하였다. 3개의 원반에 실시예의 추출물을 묻혀 시험균주인 인삼선충이 도포된 고체 한천배지에 올려놓고 배양한 후 원반 주변의 투명대 (growth inhibition halo)의 크기를 측정하는 한천 확산법으로 항균 활성을 측정하였다. 항균시험 농도를 100 ppm (0.01%)으로 한정해서 수행하였고, 무처리의 경우, 추출액을 사용하지 않은 상태의 시험결과이며, 7일간 관찰한 결과를 표 1과 같다.The antimicrobial effect against ginseng nematodes was measured using the extracts obtained in Examples 1 to 3. The extracts of the examples were buried in three disks and placed on a solid agar medium to which the test strain ginseng nematode was applied, followed by incubation, and the antimicrobial activity was measured by agar diffusion method for measuring the size of the growth inhibition halo around the disk. The antimicrobial test concentration was limited to 100 ppm (0.01%), and in the case of no treatment, the test result was obtained without using the extract, and the results observed for 7 days are shown in Table 1.
표 1
Table 1
| 선충에 대한 향균 시험 | 비고 | |
| 실시예 1의 추출물 | ++++ | |
| 실시예 3의 추출물 | +++ | |
| 무처리 | - |
| Antifungal Test for Nematodes | Remarks | |
| Extract of Example 1 | ++++ | |
| Extract of Example 3 | +++ | |
| No treatment | - |
+:투명대 크기가 2 mm 미만, ++ : 투명대 크기가 2 내지 4 mm미만, +++ : 투명대 크기가 4 내지 8 mm, ++++: 8mm 이상임.+: Transparent band size is less than 2 mm, ++: transparent band size is less than 2 to 4 mm, +++: transparent band size is 4 to 8 mm, ++++: 8 mm or more.
표1에서 알 수 있듯이 본 발명의 실시예 1의 추출물이 선충에 대한 방제효과가 실시예 3에 비해 뛰어난 것으로 나타났다.As can be seen in Table 1, the extract of Example 1 of the present invention was found to be superior to Example 3 in controlling the nematode.
2. 탄저균에 대한 항균효과2. Antibacterial effect against anthrax
표 2
TABLE 2
| 탄저균에 대한 향균 시험 | 비고 | |
| 실시예 1의 추출물 | ++++ | |
| 실시예 3의 추출물 | ++++ | |
| 무처리 | - |
| Antibacterial test for anthrax | Remarks | |
| Extract of Example 1 | ++++ | |
| Extract of Example 3 | ++++ | |
| No treatment | - |
탄저균에 대한 항균시험에서는 실시예 1 및 실시예 3의 추출물이 모두 동일한 살균 효능이 있는 것으로 나타났다.The antibacterial test for anthrax showed that the extracts of Example 1 and Example 3 had the same bactericidal efficacy.
[실험예 2]Experimental Example 2
실시예 1에서 제조된 추출물에 대하여 한천 확산법을 이용하여 무름병(Soft rot), 노균병(Downy mildew), 검은무늬병(Black spot), 검은뿌리 썩음병(Black root rot), 역병( Phytophthora rot) 대해 향균 스펙트럼을 측정하였다.The extract prepared in Example 1 was antibacterial against soft rot, Downy mildew, Black spot, Black root rot, and phytophthora rot using agar diffusion method. The spectrum was measured.
표 3
TABLE 3
| 병원균 | 향균력 |
| 무름병(Soft rot) | +++ |
| 노균병(Downy mildew) | ++++ |
| 검은무늬병(Black spot) | +++++ |
| 검은뿌리 썩음병(Black root rot) | +++++ |
| 역병( Phytophthora rot) | +++ |
| Pathogens | Antibacterial |
| Soft rot | +++ |
| Downy mildew | ++++ |
| Black spot | +++++ |
| Black root rot | +++++ |
| Plague (Phytophthora rot) | +++ |
본 발명의 추출물은 무름병,노균병 검은무늬병, 검은뿌리 썩음병 및 역병의 병원균 성장을 현저히 억제시키는 활성을 가지고 있어 이들 병원균을 방제할 수 있다.The extract of the present invention has the activity of significantly inhibiting the growth of pathogens of purine, mildew black pattern disease, black root rot and late blight, can control these pathogens.
본 발명은 광범위한 식물에 대해 우수한 살균 및 살충 효과를 나타내는 살균 살충제 조성물로, 과수류나 엽채 과채류, 근채류, 곡류 및 특용작물 등의 해충이나 선충을 효율적으로 방제한다.The present invention is a bactericidal insecticide composition exhibiting excellent bactericidal and insecticidal effects on a wide range of plants, and effectively controls pests and nematodes such as fruit trees, leafy vegetables, root vegetables, cereals and special crops.
또한, 본 발명의 살균 살충제 조성물은 상기 과수류 등의 약해를 최소화할 뿐만 아니라, 토양오염이나 잔류 농약 등을 원천적 제거하는 기능을 가지고 있으며, 모든 작물에 공통적으로 발생하는 탄저병, 노균병, 흰가루병 등의 방제효과가 뛰어난 항균 활성이 우수한 식물 추출물을 유효성분으로 포함하는 항균용 농약 조성물로서의 유익하며, 특히, 인삼뿌리에 발생하는 선충을 효율적으로 방제하는 등 친환경 농약으로서의 이용 가능성이 매우 높다.In addition, the bactericidal insecticide composition of the present invention not only minimizes the weakness of the fruit trees and the like, but also has a function of removing soil contamination or residual pesticides, and controlling anthrax, downy mildew, powdery mildew, etc. commonly occurring in all crops. It is beneficial as an antimicrobial pesticide composition comprising a plant extract having excellent antimicrobial activity as an effective ingredient, and is particularly useful as an environmentally friendly pesticide by effectively controlling nematodes generated in ginseng roots.
Claims (4)
- 협죽도 100중량부에 대하여 자리공 10 내지 30중량부, 마늘 30 내지 50중량부, 백두옹 30 내지 50중량부와 6개월간 은행껍질, 100 내지 200중량부에 증류수 700 내지 1000중량부를 60 내지 90℃를 유지하는 항온기에 넣고, 숙성시킨 추출액에 하기 식 1의 황(S)과 가성소다(NaOH)의 반응물을 혼합한 후 30 내지 1시간 동안 교반하는 것을 특징으로 하는 식물성 살균, 살충 조성물.10 to 30 parts by weight per 100 parts by weight of oleander, 30 to 50 parts by weight of garlic, 30 to 50 parts by weight of Baekduong and ginkgo shells for 6 months, and 700 to 1000 parts by weight of distilled water at 60 to 90 ° C. The plant sterilization, insecticidal composition, characterized in that the mixture of sulfur (S) and caustic soda (NaOH) of the formula 1 to the aged extract and then stirred for 30 to 1 hour.[식 1][Equation 1]4S + 6NaOH → 2Na2S + Na2S2O3+ 3H2O4S + 6NaOH → 2Na 2 S + Na 2 S 2 O 3 + 3H 2 O
- 제1항에 있어서,The method of claim 1,상기의 추출액과 상기 식 1의 유황반응물의 질량비가 3 : 1로 조성되는 것을 특징으로 식물성 살균, 살충 조성물.The mass ratio of the extract and the sulfur reactant of Formula 1 is 3: 1, characterized in that the plant sterilization, insecticidal composition.
- 제1항에 있어서,The method of claim 1,상기의 항온기에서 7 - 10일간 숙성시키는 것을 특징으로 식물성 살균, 살충 조성물.Vegetable sterilization, insecticidal composition, characterized in that aged for 7-10 days in the thermostat.
- 이물질을 제거한 협죽도을 파쇄기로 직경이 3 내지 5cm 되도록 파쇄하여 햇빛이 들지 않는 그늘에서 2 - 3일간 건조시키는 단계;Crushing the oleander removed the foreign matter to 3 to 5cm in diameter with a crusher to dry for 2-3 days in the shade without sunlight;자리공, 마늘 및 백두옹을 믹서기에 넣어 분쇄하는 단계;Crushing the pore, garlic and baekduong into a blender;은행껍질을 20 - 30℃의 온도에서 6개월간 숙성시키는 단계 Aging ginkgo shell for 6 months at a temperature of 20-30 ℃상기의 파쇄한 협죽도, 자리공, 마늘, 백두옹 및 숙성시킨 은행 껍질을 증류수와 함께 60 내지 90℃ 항온기에 넣고 7 - 10일간 숙성시키는 단계;The crushed oleander, perforated, garlic, baekduong and aged ginkgo shells with distilled water in a 60 ~ 90 ℃ constant temperature for 7-10 days;숙성이 끝난 상기의 추출물을 체로 걸러 추출액을 얻는 단계;Filtering the extract of aging after sieving to obtain an extract;상기의 추출액에 식 1의 유황 반응물을 혼합한 후 0.5 내지 1시간 동안 교반하는 단계;Mixing the sulfur reactant of Formula 1 with the extract, followed by stirring for 0.5 to 1 hour;교반이 끝난 추출액을 8시간 방치한 후 포장용기에 포장하는 단계로 구성되는 것을 특징으로 하는 식물성 살균, 살충제의 제조방법.Method of producing a vegetable sterilization, insecticide, characterized in that consisting of the step of packaging the packaging after leaving the stirred solution for 8 hours.[식 1][Equation 1]4S + 6NaOH → 2Na2S + Na2S2O3+ 3H2O4S + 6NaOH → 2Na 2 S + Na 2 S 2 O 3 + 3H 2 O
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| CN114027325A (en) * | 2021-10-14 | 2022-02-11 | 广东真格生物科技有限公司 | Sterilization composition containing fluconazole hydroxylamine, preparation and application thereof |
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| KR20210014043A (en) | 2019-07-29 | 2021-02-08 | 산사 주식회사 | 10% benzalkonium chloride disinfectant and disinfectant composition containing clove oil |
| KR102386076B1 (en) * | 2020-03-13 | 2022-04-12 | 주식회사 케이티앤지 | Antifungal Composition comprising Pulsatilla koreana extract as an active ingredient and method for controlling plant diseases using the same |
| KR102509566B1 (en) | 2020-10-13 | 2023-03-10 | 강성두 | Manufacturing method of microorganism using medical properties of a plant and obtained by fermentation of sulfur, and manufacturing method of insecticide, germicide and deodorizer using thereof |
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| KR20130011644A (en) * | 2011-07-22 | 2013-01-30 | 전한택 | Composition for controlling lawn-disease comprising plant extracts and vegetable oils as effective components |
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