WO2015112896A3 - Méthodes et compositions pour des séquences guidant le ciblage de cas9 - Google Patents

Méthodes et compositions pour des séquences guidant le ciblage de cas9 Download PDF

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Publication number
WO2015112896A3
WO2015112896A3 PCT/US2015/012747 US2015012747W WO2015112896A3 WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3 US 2015012747 W US2015012747 W US 2015012747W WO 2015112896 A3 WO2015112896 A3 WO 2015112896A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
cas9 targeting
sequences
guiding cas9
Prior art date
Application number
PCT/US2015/012747
Other languages
English (en)
Other versions
WO2015112896A9 (fr
WO2015112896A2 (fr
Inventor
Rodolphe Barrangou
Kurt M. SELLE
Alexandra E. BRINER
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Priority to JP2016547162A priority Critical patent/JP2017503514A/ja
Priority to CA2936646A priority patent/CA2936646C/fr
Priority to EP15740979.8A priority patent/EP3097212A4/fr
Priority to US15/113,656 priority patent/US10787654B2/en
Publication of WO2015112896A2 publication Critical patent/WO2015112896A2/fr
Publication of WO2015112896A3 publication Critical patent/WO2015112896A3/fr
Publication of WO2015112896A9 publication Critical patent/WO2015112896A9/fr
Priority to US17/002,133 priority patent/US20210017507A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des méthodes et des compositions pour l'édition du génome et le ciblage de l'ADN de protéines.
PCT/US2015/012747 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9 WO2015112896A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2016547162A JP2017503514A (ja) 2014-01-24 2015-01-23 Ｃａｓ９ターゲッティングをガイドする配列に関する方法および組成物
CA2936646A CA2936646C (fr) 2014-01-24 2015-01-23 Methodes et compositions pour des sequences guidant le ciblage de cas9
EP15740979.8A EP3097212A4 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9
US15/113,656 US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
US17/002,133 US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461931515P 2014-01-24 2014-01-24
US61/931,515 2014-01-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/113,656 A-371-Of-International US10787654B2 (en) 2014-01-24 2015-01-23 Methods and compositions for sequence guiding Cas9 targeting
US17/002,133 Division US20210017507A1 (en) 2014-01-24 2020-08-25 Methods and compositions for sequences guiding cas9 targeting

Publications (3)

Publication Number Publication Date
WO2015112896A2 WO2015112896A2 (fr) 2015-07-30
WO2015112896A3 true WO2015112896A3 (fr) 2015-10-29
WO2015112896A9 WO2015112896A9 (fr) 2015-11-26

Family

ID=53682132

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/012747 WO2015112896A2 (fr) 2014-01-24 2015-01-23 Méthodes et compositions pour des séquences guidant le ciblage de cas9

Country Status (4)

Country Link
EP (1) EP3097212A4 (fr)
JP (4) JP2017503514A (fr)
CA (1) CA2936646C (fr)
WO (1) WO2015112896A2 (fr)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (fr) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Évaluation et amélioration de la spécificité de clivage des nucléases
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
AU2014308900A1 (en) 2013-08-22 2016-03-10 E. I. Du Pont De Nemours And Company A soybean U6 polymerase III promoter and methods of use
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US10584358B2 (en) 2013-10-30 2020-03-10 North Carolina State University Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri
LT3066201T (lt) 2013-11-07 2018-08-10 Editas Medicine, Inc. Su crispr susiję būdai ir kompozicijos su valdančiomis grnr
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
EP3097212A4 (fr) * 2014-01-24 2017-10-04 North Carolina State University Méthodes et compositions pour des séquences guidant le ciblage de cas9
CN106460003A (zh) 2014-04-08 2017-02-22 北卡罗来纳州立大学 用于使用crispr相关基因rna引导阻遏转录的方法和组合物
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3633032A3 (fr) 2014-08-28 2020-07-29 North Carolina State University Nouvelles protéines cas9 et éléments de guidage pour le ciblage de l'adn et l'édition du génome
CA2976387A1 (fr) 2015-03-27 2016-10-06 E I Du Pont De Nemours And Company Promoteurs de gene d'arn nucleaire de petite taille u6 de soja et leur utilisation dans l'expression constitutive de genes d'arn de petite taille dans des plantes
CN107709562A (zh) * 2015-05-15 2018-02-16 先锋国际良种公司 指导rna/cas内切核酸酶***
CN108026536A (zh) 2015-05-29 2018-05-11 北卡罗来纳州立大学 使用crispr核酸筛选细菌、古细菌、藻类和酵母的方法
FI3307872T3 (fi) 2015-06-15 2023-10-16 Univ North Carolina State Menetelmiä ja koostumuksia nukleiinihappojen ja rna-pohjaisten antimikrobien tehokkaaseen antamiseen
US9580727B1 (en) 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
WO2017058751A1 (fr) 2015-09-28 2017-04-06 North Carolina State University Méthodes et compositions pour agents antimicrobiens spécifiques d'une séquence
CN108699116A (zh) 2015-10-23 2018-10-23 哈佛大学的校长及成员们 用于基因编辑的演化的cas9蛋白
US11542466B2 (en) 2015-12-22 2023-01-03 North Carolina State University Methods and compositions for delivery of CRISPR based antimicrobials
CN105594664B (zh) * 2016-02-16 2018-10-02 湖南师范大学 一种基因敲除选育stat1a基因缺失型斑马鱼的方法
WO2018027078A1 (fr) 2016-08-03 2018-02-08 President And Fellows Of Harard College Éditeurs de nucléobases d'adénosine et utilisations associées
EP3497214B1 (fr) 2016-08-09 2023-06-28 President and Fellows of Harvard College Protéines de fusion cas9-recombinase programmables et utilisations associées
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CA3039928A1 (fr) 2016-10-14 2018-04-19 President And Fellows Of Harvard College Administration d'aav d'editeurs de nucleobases
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
WO2018165504A1 (fr) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression de la douleur par édition de gène
EP3592777A1 (fr) 2017-03-10 2020-01-15 President and Fellows of Harvard College Éditeur de base cytosine à guanine
US11268082B2 (en) 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
EP3630975A4 (fr) * 2017-05-26 2021-03-10 North Carolina State University Arn guides modifiés pour moduler l'activité cas9 et procédés d'utilisation
EP3652312A1 (fr) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
CN112020554A (zh) * 2018-02-23 2020-12-01 先锋国际良种公司 新颖cas9直系同源物
EP3861120A4 (fr) 2018-10-01 2023-08-16 North Carolina State University Système crispr-cas de type i recombinant
CA3117228A1 (fr) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Nouveaux systemes crispr-cas d'edition du genome
WO2020191243A1 (fr) 2019-03-19 2020-09-24 The Broad Institute, Inc. Procédés et compositions pour l'édition de séquences de nucléotides
CN110117622B (zh) * 2019-05-24 2021-05-11 青岛农业大学 一种CRISPR/Cas基因编辑***及其制备方法和应用
WO2020263773A1 (fr) * 2019-06-24 2020-12-30 North Carolina State University Procédés et compositions pour modifier des génomes bactériens et pour tuer des bactéries
WO2021216772A1 (fr) * 2020-04-21 2021-10-28 Mammoth Biosciences, Inc. Nucléases programmables casy et systèmes de composants d'arn
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013176772A1 (fr) * 2012-05-25 2013-11-28 The Regents Of The University Of California Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3097212A4 (fr) * 2014-01-24 2017-10-04 North Carolina State University Méthodes et compositions pour des séquences guidant le ciblage de cas9

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013176772A1 (fr) * 2012-05-25 2013-11-28 The Regents Of The University Of California Procédés et compositions permettant la modification de l'adn cible dirigée par l'arn et la modulation de la transcription dirigée par l'arn

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CONG ET AL.: "Multiplex genome engineering using CRISPR/Cas systems.", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 819 - 823, XP055181426 *

Also Published As

Publication number Publication date
JP7429057B2 (ja) 2024-02-07
WO2015112896A9 (fr) 2015-11-26
JP2017503514A (ja) 2017-02-02
CA2936646A1 (fr) 2015-07-30
WO2015112896A2 (fr) 2015-07-30
JP2024041934A (ja) 2024-03-27
EP3097212A4 (fr) 2017-10-04
JP2022106883A (ja) 2022-07-20
EP3097212A2 (fr) 2016-11-30
CA2936646C (fr) 2024-04-30
JP2020103295A (ja) 2020-07-09

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