WO2015078398A1 - 一种用于紫外损伤修复的中草药复方及其在化妆品中应用 - Google Patents

一种用于紫外损伤修复的中草药复方及其在化妆品中应用 Download PDF

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WO2015078398A1
WO2015078398A1 PCT/CN2014/092435 CN2014092435W WO2015078398A1 WO 2015078398 A1 WO2015078398 A1 WO 2015078398A1 CN 2014092435 W CN2014092435 W CN 2014092435W WO 2015078398 A1 WO2015078398 A1 WO 2015078398A1
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peach
extract
peach kernel
compound extract
weight
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PCT/CN2014/092435
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English (en)
French (fr)
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孙懿
方程
赵亚
陈默
祝乐
戴竟
吕洛
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上海家化联合股份有限公司
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/004—Aftersun preparations

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  • the invention relates to a Chinese herbal compound for ultraviolet damage repair and its application in cosmetics, in particular to a peach peach kernel compound and its application in cosmetics.
  • Ultraviolet rays not only damage the outermost skin cells, but also cause dermal cell necrosis and metabolic disorders.
  • the impurities in the cells cannot be metabolized, causing sunburn, loss of elasticity, premature aging, sensitivity, wrinkles, and skin.
  • the tingling and itching are unbearable, scratching the bleeding, the skin's own resistance to disease and resistance is weakened, and even edema, exudation, formation of folliculitis, sputum and other difficult to cure skin diseases.
  • the understanding of the mechanism of ultraviolet light on skin damage includes oxidative stress, inflammatory reaction, apoptosis, degradation of collagen and elastin.
  • the skin tissue cells will produce oxidative stress, produce excessive reactive oxygen species ROS, destroy the integrity of the cell membrane and the anti-oxidation system inside the cell, further causing cell inflammation, apoptosis and tumor formation.
  • ROS reactive oxygen species
  • excessive ultraviolet radiation causes skin collagen, hyaluronic acid and elastin to degrade, resulting in elastic degeneration, roughness and wrinkles. Therefore, most of the repair methods after the ultraviolet ray damage are based on these mechanisms, and substances having antioxidation, anti-inflammatory, and matrix metalloproteinase inhibition activities are used.
  • ash tree extract effectively removes a large number of free radicals generated in human Fb cells after UVB irradiation, and reduces the expression of MMP-1 mRNA and protein, thereby resisting skin damage after UVB irradiation; for example, marigold flower extract contains ⁇ Carotene, which inhibits the production of wrinkles by inhibiting the activity of hyaluronidase, elastase, and MMP-1, thereby exerting an ultraviolet protection effect.
  • ATP is a key molecule in the metabolism of cell energy. It is the main energy source that skin cells can directly use. It is also an important indicator of the healthy viability of cells. The reduction of ATP leads to the reduction of energy metabolism, and the corresponding functional decline. The most obvious is skin aging, dull acne and so on. New research has found that intense exposure to ultraviolet light can cause a decrease in ATP production, leading to a decrease in energy metabolism and affecting the normal function of skin cells. The increase of ATP can promote the increase of cells after injury, and promote Into the metabolism of cell energy, which is conducive to the repair of skin damage caused by ultraviolet radiation.
  • Peach blossom is the flower of the Prunus persica (L.) Batsch of the Rosaceae plant. The bitterness is flat and the water is swollen.
  • the "Shen Nong's Herbal Classic" records that the peach blossom is "a good color”; the peach kernel is the dry ripe of the Prunus persica (L.) Batsch or the Prunus davidiana (Carr.) Franch. seed. It tastes bitter and sweet, promotes blood circulation, and can moisturize dryness.
  • the two are compatible, activating blood circulation, moistening dryness and diuresis, and can make blood stasis, water and swollen body restore blood and blood circulation, and eliminate qi and blood stasis in the body.
  • the prior art describes the research progress of the skin damage mechanism of ultraviolet rays and natural products having ultraviolet protection.
  • the research progress of the skin damage mechanism of ultraviolet light and the UV-protectant of natural sources is reviewed. Specifically pointed out: long-term exposure of the skin to ultraviolet light can cause skin damage, skin photoaging and even skin cancer and other skin lesions.
  • the main mechanisms of photoaging caused by ultraviolet light are: oxidative stress, inflammatory reaction, apoptosis, degradation of collagen and elastin.
  • UV protective agents At present, flavonoids, terpenoids, tannins and coumarins are mainly studied.
  • Chinese patent application 201110198166.5 discloses a compound Chinese medicine extract and its application in whitening moisturizing anti-aging skin care products. This document mainly discloses a compound Chinese medicine extract and its application in whitening moisturizing anti-aging skin care products.
  • the compound Chinese medicine extract is prepared from the following raw materials by mass: 10 to 50 parts of Selaginella, 10 to 50 parts of peach, 10 to 50 parts of white and 10 to 50 parts of notoginseng.
  • the compound Chinese herbal extract obtained by the above raw materials has an inhibitory effect on the proliferation of tyrosinase and melanocytes, has an excellent whitening effect, and has a promoting effect on proliferation of fibroblasts; in epidermal cell culture, the above-mentioned compound Chinese herbal extracts
  • the production of ceramide has a good promotion effect.
  • the compound Chinese medicine extract is applied to prepare a whitening moisturizing anti-aging skin care product, and the obtained whitening moisturizing anti-aging skin care product has the lasting natural whitening effect, and can improve the metabolism and moisturizing function of the skin.
  • This patent application while also revealing a composition, is not a composition of the peach kernel of the present invention.
  • Cisoka leaf spotting beauty tea and a preparation method thereof discloses a mulberry leaf spotting beauty tea and a preparation method thereof.
  • a mulberry leaf spotting beauty tea and a preparation method thereof are provided, and various raw materials are: mulberry leaves, peach blossoms, raspberries, alfalfa, spatholobus, codonopsis, perrin, gynostemma, angelica, polygonatum, peach kernel, turmeric, incense Attachment, white peony root, Chuanxiong, Danshen, Coix seed, Atractylodes, Peach blossom, White and, Ligustrum lucidum, Campsis, white peony.
  • the invention solves the defects that the mulberry leaf tea is easy to be stuffy yellow, brittle, and the soup color is red, and the polyphenols in the mulberry leaves are exhausted. It may be converted into amino acid, which is beneficial to remove the bitter taste in tea and make it more delicious.
  • the mulberry leaf contains mulberry glycoside F, which has inhibitory effect on the biosynthesis of melanin.
  • mulberry leaf juice has anti-oxidation effect, non-toxic, no Chemical pollution, can be used for facial acne, pigmentation, phlegm, pharyngeal, hair growth, hair care, liver and eyesight treatment, plus some Chinese herbal medicines that promote blood circulation and phlegm, most of these herbs are light, do not affect mulberry tea
  • the taste is easy to accept, and it can better achieve the beauty and remove the pigmentation.
  • the application is directed to mulberry leaf compositions, although peach and peach kernels are added to it, but it is not a major Chinese herbal ingredient.
  • the prior art does not mention the role of the peach peach kernel compound in repairing UVB radiation damage and promoting energy metabolism.
  • the present invention finds for the first time that the peach peach kernel compound has the properties of ultraviolet damage repair and promoting skin cell energy metabolism.
  • the invention aims to provide a Chinese herbal compound extract with ultraviolet damage repair, which can promote ATP synthesis and repair ultraviolet damage caused by UVB. At the same time, it can be added as an efficacy additive to cosmetics for skin care after UV injury.
  • peach peach kernel The extraction process of peach peach kernel is as follows:
  • Peach medicinal materials are soaked in a suitable solvent for about 0.5-1 h, and extracted by the corresponding method.
  • the filtrate is taken, statically filtered, concentrated, and dried to form a powder.
  • the peach kernel is soaked in a suitable solvent for about 0.5-1 h, and extracted by the corresponding method.
  • the filtrate is taken, statically filtered, concentrated, and dried to form a powder.
  • the peach blossom extract and the peach kernel extract were mixed in proportion, and finally a 1% by weight composite extract solution was prepared.
  • the Chinese herbal medicines in the formula may be appropriately enlarged or reduced according to the proportion; suitable solvents include water (distilled water, deionized water, purified water), different concentrations of ethanol solution, different concentrations of polyol solution and other solvents of similar polarity; the extraction method may It is a method of boiling, reflux extraction, dipping, seepage, and the like.
  • the preparation of the compound extract of Peach Blossom Peach can also be carried out by supercritical extraction, subcritical extraction and organic solvent extraction.
  • Solvents for organic solvent extraction include n-hexane, ethyl acetate, diethyl ether, petroleum ether, Medium and low polarity solvents such as acetone, and different concentrations of ethanol, methanol, and polyols.
  • the extraction method may be a method such as reflux extraction, impregnation, or osmosis.
  • ultrasonic or microwave methods can also be used for assisting during the extraction process.
  • the present invention relates to the use of a peach peach kernel compound extract for the repair of ultraviolet damage and the promotion of energy metabolism.
  • the present invention relates to the use of a peach peach kernel compound extract as an additive having ultraviolet damage repair and promoting energy metabolism in cosmetics.
  • the peach peach kernel compound extract is used in an amount of 0.00001 to 100% by weight. In another embodiment, the peach peach kernel compound extract is used in an amount of 0.0001 to 50% by weight. In a preferred embodiment, the peach peach kernel compound extract is used in an amount of from 0.001% to 20% by weight.
  • the cosmetic of the present invention is selected from the group consisting of facial cleansers, lotions, lotions, creams, gels, masks, shampoos, shower gels, and hair conditioners.
  • the cosmetic of the present invention comprises a peach peach kernel compound extract and an acceptable adjuvant.
  • the cosmetic comprises from 0.00001 to 100% by weight of a peach peach kernel compound extract. In another embodiment, the cosmetic comprises 0.0001 to 50% by weight of a peach peach kernel compound extract. In another embodiment, the cosmetic comprises from 0.001% to 20% by weight of the peach peach kernel compound extract.
  • Figure 1 shows the percentage of ATP content of normal cells, UV irradiated cells, and various concentrations of the peach extract of the present invention.
  • # indicates a significant difference in the UVB irradiation group with respect to the normal group; ** indicates a significant difference (p ⁇ 0.01) with respect to the UVB irradiation group.
  • the invention firstly proposes that the peach peach kernel compound has the effects of ultraviolet damage repair and energy metabolism.
  • Peach blossom peach kernel complex provided by the invention The extract can repair the energy loss of cells after UVB irradiation, promote the energy metabolism of skin cells and the synthesis of ATP, which is beneficial to the repair of UV damage.
  • the inventors of the present invention found that the peach peach kernel compound extract has ultraviolet damage repair and promotes energy metabolism.
  • the peach peach kernel compound extract can be added to cosmetics as an efficacy additive.
  • the present invention firstly proposes that the peach peach kernel compound extract has ultraviolet damage repair and promotes energy metabolism, and can be added as a functional additive to cosmetics for ultraviolet damage repair and skin cell energy supplementation.
  • the peach peach kernel compound extract can be in various forms.
  • the application form of the peach peach kernel compound extract may be a cleansing lotion, a lotion, a lotion, a cream, a gel, a mask, a shampoo, a shower gel, a conditioner, and the like which can be applied to human skin.
  • ingredients other than the peach peach kernel extract may be any ingredients required for a cosmetic formulation, such as: water, ethanol, butylene glycol, propylene glycol, oils, emulsifiers, thickeners, preservatives, etc.; Compound functions, such as: adding sunscreen ingredients to promote energy metabolism, sunscreen cosmetics, adding oil control ingredients to promote energy metabolism, oil control cosmetics, adding moisturizing ingredients to promote energy metabolism, moisturizing cosmetics, adding whitening ingredients to promote energy metabolism, whitening cosmetics, adding anti- The aging component becomes an anti-aging cosmetic that promotes energy metabolism and the like.
  • the peach peach kernel compound extract can be used at a concentration of 0.00001-100% by weight.
  • the peach peach kernel compound extract can be used at a concentration of 0.0001 to 50% by weight.
  • the peach peach kernel compound extract is used at a concentration of 0.00001 to 100% by weight.
  • the peach peach kernel compound extract has a concentration of from 0.001% to 50% by weight.
  • the peach peach kernel compound extract is used at a concentration of from 0.001% to 20% by weight.
  • the peach peach kernel compound extract is used at a concentration of from 0.001 to 100 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.001-50 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.001 to 10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of from 0.01 to 10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.1-10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 1-10 ug/ml.
  • the peach peach kernel compound extract can be added to various cosmetics in a ratio of 0.00001-100% by weight.
  • the peach peach kernel compound extract may be used in an amount of 0.00001 to 25% by weight, for example 0.0001 to 50% by weight, for example 0.001 to 75% by weight, for example 0.1 to 100% by weight.
  • the peach peach kernel compound extract in the cosmetic is used in an amount of 0.0001 to 50% by weight.
  • the peach peach kernel compound extract is used in the cosmetic in an amount of 0.001 to 20% by weight.
  • the weight ratio of the peach flower extract and the peach kernel extract in the peach peach kernel compound extract may be any ratio.
  • the weight ratio of peach blossom extract and peach kernel extract in the peach peach kernel compound extract is 3:7.
  • Example 1 Repair of skin cell energy damage caused by UVB irradiation of peach peach kernel
  • a 1% by weight peach peach kernel extract was removed and diluted to 10 ug/ml, 1 ug/ml, and 0.1 ug/ml with PBS buffer to obtain a sample solution.
  • the adjusted concentration is inoculated into the cell plate.
  • the cells were placed under a UVB irradiation apparatus, and normal medium and medium containing the extract of the example were added to continue the culture incubation.
  • test solution in the kit After incubation for a period of time, add the test solution in the kit to each well, shake, and stabilize the fluorescence at room temperature. The instrument will detect the reading.
  • the fluorescence unit RLU measured in each well was recorded, and the average of 3 replicate wells in each group was averaged.
  • the experimental results were subjected to t test, P ⁇ 0.05, indicating significant difference; p ⁇ 0.01 indicates that the difference was very significant.
  • the percentage value of ATP content of each unit of fluorescence was calculated based on 100% of normal cells.
  • Peach Blossom Peach has UV damage repair and promoted cell energy metabolism. Even at low concentrations (for example, 0.1 ug / ml concentration), Peach Blossom Peach Compound extract has a good repair effect on cell energy loss after UVB UV irradiation, promotes the synthesis of ATP, and maintains the energy metabolism level of skin cells. As shown in Table 1 and Figure 1, the repair rate can reach more than 75% at 10ug/ml, and the repair rate can reach nearly 50% at a low dose concentration of 0.1ug/ml.
  • Peach peach kernel extract concentration Protection rate (%) 10ug/ml 76.43% 1ug/ml 59.19% 0.1ug/ml 48.15%
  • An energy metabolism anti-aging cream comprising the peach peach kernel compound extract of the present invention was prepared according to the ingredients and amounts of Table 2.
  • the preparation method comprises the steps of: dissolving the peach peach extract with 1,3-butanediol, adding it to water, and stirring uniformly to obtain an aqueous phase.
  • the oil phase is slowly added to the water phase, stirring is continued, and the preservative and the essence are added after being evenly mixed, and the mixture is uniformly obtained.
  • the energy-enhancing moisturizing water containing the peach peach kernel compound extract of the present invention was prepared according to the ingredients and amounts of Table 3.
  • Preparation method first dissolve the peach peach kernel extract with 1,3-butanediol, then add to the water, stir, evenly add the preservative and flavor, and stir evenly.

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Abstract

桃花桃仁复方提取物在紫外损伤修复和促进能量代谢中的用途，以及作为具有紫外损伤修复和促进能量代谢作用的添加剂在化妆品中的用途。

Description

一种用于紫外损伤修复的中草药复方及其在化妆品中应用 技术领域
本发明涉及一种用于紫外损伤修复的中草药复方及其在化妆品中应用，具体涉及桃花桃仁复方及其在化妆品中的应用。
背景技术
紫外线不仅仅破坏最外层的皮肤细胞，同时还能导致真皮细胞坏死和代谢紊乱，细胞内杂质无法代谢出去，令肌肤出现晒斑，丧失弹性，提早衰老，变得敏感，出现皱纹，而且皮肤损伤后刺痛瘙痒难忍，抓挠出血，皮肤本身的抗病和抵抗能力减弱，甚至水肿，渗出，形成毛囊炎，疖等难以治愈的皮肤病。
目前对紫外线对皮肤损伤的机理认识有：氧化应激、炎症反应、细胞凋亡、胶原和弹性蛋白的降解。皮肤接受过多的紫外线照射，皮肤组织细胞会产生氧化应激，产生过量的活性氧簇ROS，破坏细胞膜的完整性和细胞内的抗氧化***，进一步引起细胞炎症、细胞凋亡及肿瘤的形成。同时过量的紫外线照射使皮肤胶原、透明质酸、弹性蛋白降解，导致皮肤弹性变性、粗糙、形成皱纹。因此，对于紫外线损伤后的修复方法大多是从这些机理出发，使用具有抗氧化、抗炎、抑制基质金属蛋白酶作用的物质。例如白蜡树提取物有效的清除UVB照射后人Fb细胞中生成的大量自由基，降低MMP-1的mRNA和蛋白的表达，从而对抗UVB照射后的皮肤损伤；又比如万寿菊花提取物中含有β胡萝卜素，其通过抑制透明质酸酶、弹性蛋白酶、MMP-1的活性，抑制皱纹产生，从而起到紫外线防护作用。
ATP是细胞能量新陈代谢的关键分子，是皮肤细胞可以直接使用的主要能量源，也是反应细胞健康活力的一项重要指标，ATP的减少，导致能量代谢的减弱，机体会相应的出现功能性的衰退，最明显的莫过于皮肤衰老、暗沉痤疮等。新的研究发现紫外线的强烈照射，能引起ATP生成减少，导致能量代谢的减弱，影响皮肤细胞正常功能。ATP的增加能促进损伤后细胞的增加，促 进细胞能量的新陈代谢，从而有利于紫外线照射造成的皮肤损伤进行修复。
桃花为蔷薇科植物桃Prunus persica(L.)Batsch的花。味苦性平，利水消肿，《神农本草经》里记载桃花"令人好颜色"；桃仁为蔷薇科植物桃Prunus persica(L.)Batsch或山桃Prunus davidiana(Carr.)Franch.的干燥成熟种子。味苦甘性平，活血化瘀，兼能润燥。二者相配伍，活血化瘀、润燥利水，能令血瘀、水饮肿满之体恢复气血通调，排除体内气血水瘀滞。
现有技术记载了紫外线的皮肤损伤机制及具有紫外线防护作用的天然产物的研究进展。主要针对紫外线的皮肤损伤机制和天然来源紫外线防护剂的研究进展进行了综述。具体指出：皮肤长期暴露在紫外线下可以引起皮肤损伤，出现皮肤光老化甚至出现皮肤癌变和其他皮肤病变。紫外线引起的皮肤光老化的主要机制有：氧化应激、炎症反应、细胞凋亡、胶原和弹性蛋白的降解等。目前国内外关注的焦点是开发紫外线防护剂，目前主要研究的有黄酮类化合物、萜类化合物、鞣质类和香豆素类等。但是，已有的报道均未提到促进能量代谢和ATP的合成也能够对UVB进行修复。
中国专利申请201110198166.5揭示了一种复方中药提取物及其在美白保湿抗衰老护肤品中的应用。该文献主要公开了一种复方中药提取物及其在美白保湿抗衰老护肤品中的应用。该复方中药提取物由以下按质量份计的原料药制备而成:卷柏10～50份、桃花10～50份、白及10～50份和三七10～50份。前述原料得到的复方中药提取物对酪氨酸酶和黑色素细胞的增殖具有抑制作用，具有优异的美白效果；对纤维芽细胞有增殖有促进作用；在表皮细胞培养中，上述复方中药提取物对脑酰胺的生成有很好的促进作用。将该复方中药提取物应用于制备美白保湿抗衰老护肤品,得到的美白保湿抗衰老护肤品具有持久自然美白效果,能提高肌肤新陈代谢与保湿的功能。该专利申请虽然也揭示了一种组合物，但并不是本发明所述桃花桃仁的组合物。
中国专利申请201210312256.7揭示了一种桑叶祛斑养颜茶及其制备方法。具体提供一种桑叶祛斑养颜茶及其制备方法，各种原料为：桑叶、桃花、覆盆子、茯苓、鸡血藤、党参、佩兰、绞股蓝、当归、玉竹、桃仁、郁金、香附、白芷、川芎、丹参、薏仁、白术、地桃花、白及、女贞子、凌霄花、白芍。本发明解决了桑叶茶易闷黄，易碎，汤色发红的缺点，且使桑叶中的多酚物质尽 可能转换成氨基酸，有利于去除茶中苦涩味，使其更鲜美；桑叶中含有桑皮苷F，对黑色素的生物合成有抑制作用，研证实桑叶汁具有抗氧化作用，无毒，无化学污染，可用于面部痤疮、色斑、祛痰、利咽、生发、护发、清肝明目的治疗，再加上一些活血化瘀的中药材，这些药材大多口感清淡，不影响桑叶茶味道，使人易于接受，更能较好的达到美肤养颜，去除色斑的功效。同样地，该申请针对的是桑叶组合物，虽然里面加入了桃花、桃仁，但并不是主要的中草药成分。
由此可见，现有技术均未提及桃花桃仁复方在UVB照射损伤修复和促进能量代谢方面的作用。本发明首次发现桃花桃仁复方具有紫外损伤修复和促进皮肤细胞能量代谢方面的性质。
发明内容
本发明目的在于提供一种具有紫外损伤修复的中草药复方提取物，该中草药复方提取物能够促进ATP合成，对UVB造成的紫外线损伤进行修复。同时可作为功效添加剂加入到化妆品中使用，用于紫外损伤后皮肤护理。
桃花桃仁提取工艺流程如下：
将桃花药材以适宜溶媒浸泡约0.5-1h，以相应方法提取，取滤液，静置过滤，浓缩，干燥成粉。
将桃仁药材以适宜溶媒浸泡约0.5-1h，以相应方法提取，取滤液，静置过滤，浓缩，干燥成粉。
将桃花提取物与桃仁提取物按比例混合，最终制成含1％重量的复合提取物溶液。
配方中中药材可按照比例适当放大或缩小；适宜溶媒包括水(蒸馏水、去离子水、纯净水)，不同浓度的乙醇溶液，不同浓度的多元醇溶液以及其他极性相似的溶剂；提取方法可以是煎煮、回流提取、浸渍、渗漉等方法等。
桃花桃仁复方提取物的制取也可采取超临界萃取、亚临界萃取、有机溶剂提取法。有机溶剂提取的溶剂包括正己烷、乙酸乙酯、***、石油醚、 丙酮等中低极性溶剂以及不同浓度的乙醇、甲醇以及多元醇等。提取方法可以是回流提取、浸渍、渗漉等方法。而且，提取过程中还可以使用超声或微波的方法进行辅助。
第一方面，本发明涉及桃花桃仁复方提取物在紫外损伤修复和促进能量代谢中的用途。
第二方面，本发明涉及桃花桃仁复方提取物作为具有紫外损伤修复和促进能量代谢作用的添加剂在化妆品中的用途。
在一个实施方式中，桃花桃仁复方提取物的用量在0.00001-100重量％。在另一个实施方式中，桃花桃仁复方提取物的用量在0.0001-50重量％。在优选的实施方式中，桃花桃仁复方提取物的用量为0.001％-20重量％。
本发明的化妆品选自：洁面乳、化妆水、乳液、膏霜、啫喱、面膜、洗发水、沐浴露、护发素。在一个实施方式中，本发明的化妆品包含桃花桃仁复方提取物以及可接受的辅料。
在一个实施方式中，化妆品包含0.00001-100重量％的桃花桃仁复方提取物。在另一个实施方式中，化妆品包含0.0001-50重量％的桃花桃仁复方提取物。在另一个实施方式中，化妆品包含0.001％-20重量％的桃花桃仁复方提取物。
附图说明
图1显示了正常细胞、UV照射细胞以及各种浓度的本发明桃花桃仁复方提取物的ATP含量百分比。图中，#表示相对于正常组，UVB照射组有显著差异；**表示相对于UVB照射组，具有显著差异(p<0.01)。
具体实施方式
目前针对紫外线损伤后的修复大多从氧化应激、DNA的修复、炎症反应、细胞凋亡、胶原和弹性蛋白的降解等角度出发，提供相应的解决方案。尚未有从ATP能量代谢角度提出紫外线损伤修复的解决方案。本发明首次提出桃花桃仁复方具有紫外损伤修复和促进能量代谢的作用。本发明提供的桃花桃仁复 方提取物能够对UVB照射后的细胞能量损失进行修复，促进皮肤细胞能量代谢和ATP的合成，从而有利于紫外损伤修复。
本发明的发明人在经过了广泛而深入的研究之后发现：桃花桃仁复方提取物具有紫外损伤修复和促进能量代谢作用。
因此，桃花桃仁复方提取物可作为功效添加剂加入到化妆品中。
文献中均未涉及桃花桃仁复方提取物具有紫外损伤修复和促进能量代谢作用，也未提及将其作为功能添加剂加入化妆品中。因此，本发明首次提出桃花桃仁复方提取物具有紫外损伤修复和促进能量代谢作用，可以作为功能添加剂加入化妆品中，用于紫外损伤修复及皮肤细胞能量的补充。
在化妆品应用中，桃花桃仁复方提取物可以是各种形式。例如，桃花桃仁复方提取物应用形式可以是洁面乳、化妆水、乳液、膏霜、啫喱、面膜、洗发水、沐浴露、护发素等任何可以应用于人体皮肤的剂型。
桃花桃仁复方提取物以外的其他成分可以是化妆品配方需要的任何成分，如：水、乙醇、丁二醇、丙二醇、油脂、乳化剂、增稠剂、防腐剂等；也可以添加其他功效成分达到复合功能，如：添加防晒成分使成为促进能量代谢防晒化妆品，添加控油成分使成为促进能量代谢控油化妆品，添加保湿成分成为促进能量代谢保湿化妆品，添加美白成分使成为促进能量代谢美白化妆品，添加抗衰老成分成为促进能量代谢抗衰老化妆品等等。
一般而言，桃花桃仁复方提取物的使用浓度可以在0.00001-100重量％。优选地，桃花桃仁复方提取物的使用浓度可以在0.0001-50重量％。在本发明的一个实施方式中，桃花桃仁复方提取物的使用浓度在0.00001-100重量％。在另一个实施方式中，桃花桃仁复方提取物的浓度在0.001-50重量％。在一个实施方式中，桃花桃仁复方提取物的使用浓度为0.001-20重量％。
在一个实施方式中，桃花桃仁复方提取物的使用浓度为0.001-100ug/ml。在另一个实施方式中，桃花桃仁复方提取物的使用浓度为0.001-50ug/ml。在另一个实施方式中，桃花桃仁复方提取物的使用浓度为0.001-10ug/ml。在另一个实施方式中，桃花桃仁复方提取物的使用浓度为0.01-10ug/ml。在另一个实施方式中，桃花桃仁复方提取物的使用浓度为0.1-10ug/ml。在另一个实施方式中，桃花桃仁复方提取物的使用浓度为1-10ug/ml。
可以将桃花桃仁复方提取物按照0.00001-100重量％的比例加到各种化妆品中。例如，桃花桃仁复方提取物的用量可以是0.00001-25重量％，例如0.0001-50重量％，例如0.001-75重量％，例如0.1-100重量％。在一个优选的实施方式中，化妆品中桃花桃仁复方提取物的用量为0.0001-50重量％。在另一个优选的实施方式中，化妆品中桃花桃仁复方提取物的用量为0.001-20重量％。
桃花桃仁复方提取物中桃花提取物和桃仁提取物的重量比可以是任意比例。例如，在一个实施方式中，桃花桃仁复方提取物中桃花提取物和桃仁提取物的重量比为3:7。
下面结合具体的实施例进一步阐述本发明。但是，应该明白，这些实施例仅用于说明本发明而不构成对本发明范围的限制。下列实施例中未注明具体条件的试验方法，通常按照常规条件，或按照制造厂商所建议的条件。除非另有说明，所有的百分比和份数按重量计。
实施例1：桃花桃仁对UVB照射造成的皮肤细胞能量损伤修复实验
实验样品制备：
称取桃花药材适量，以15倍去离子水提取→过滤，滤液浓缩，干燥成粉得到桃花提取物。
称取桃仁药材适量，以12倍乙醇浸泡过夜→过滤，滤液浓缩，干燥成粉，得到桃仁提取物。
将桃花提取物3份，另取桃仁提取物7份，按比例混合，最终制成含1％重量的复合提取液。
移取浓度为1％重量的桃花桃仁复合提取液，用PBS缓冲液稀释至10ug/ml、1ug/ml、0.1ug/ml，得到样品溶液。
实验方法：
1)细胞复苏，待生长状态良好时，进行细胞同步化处理。
2)同步化后调整浓度接种于细胞板中。第二天，置于UVB照射装置下，再加入正常培养基和含有实施例提取物的培养基，继续培养孵育。
3)经过一段时间孵育后，每孔加入试剂盒中测试液，震荡，室温放置稳定荧光，仪器检测读数。
实验结果：
记录下各孔测得的荧光单位RLU，每组3个复孔取平均值，实验结果进行t检验，P<0.05，表示差异显著；p<0.01表示差异非常显著。同时，以正常细胞100％为基准，计算各组荧光单位ATP含量百分比数值。
结果表明：桃花桃仁复方提取物具有紫外损伤修复和促进细胞能量代谢作用。即使在低浓度下(例如，0.1ug/ml的浓度下)，桃花桃仁复方提取物对UVB紫外照射后的细胞能量损失具有较好的修复作用，促进ATP的合成，维持皮肤细胞能量代谢水平。如表1及图1所示，在10ug/ml时修复率可到75％以上，在0.1ug/ml的低剂量浓度下也能达到近50％的修复率。
表1：桃花桃仁复方提取物的紫外损伤修复作用
桃花桃仁提取液浓度 防护率(％)
10ug/ml 76.43％
1ug/ml 59.19％
0.1ug/ml 48.15％
实施例2：促进能量代谢抗衰老膏霜的制备
按照表2的成分和用量制备包含本发明所述桃花桃仁复方提取物的促进能量代谢抗衰老膏霜。
表2
成分 ％浓度(重量)
桃花桃仁提取物 1.0
甘油硬脂酸酯柠檬酸酯 4.0
甘油硬脂酸酯 2.0
鲸蜡硬脂醇 2.0
矿物油 10.0
辛酸/癸酸甘油三酯 5.0
1,3-丁二醇 7.0
防腐剂、香精 适量
水 至100.0
制备方法：先用1,3-丁二醇溶解桃花桃仁提取物，然后加入到水中，搅拌均匀，得到水相。将甘油硬脂酸酯柠檬酸酯、甘油硬脂酸酯、鲸蜡硬脂醇、辛酸/癸酸甘油三酯、矿物油加入油相锅中，加热至75℃-80℃，搅拌均匀，得到油相。将油相缓慢加入水相中，并不停搅拌，均匀后加入防腐剂和香精，搅拌均匀，即得。
实施例3：促进能量代谢保湿水的制备
按照表3的成分和用量制备包含本发明所述桃花桃仁复方提取物的促进能量代谢保湿水。
表3
成分 ％浓度(重量)
桃花桃仁提取物 2.0
1，3-丁二醇 8.0
防腐剂、水溶性香精 适量
水 至100
制备方法：先用1,3-丁二醇溶解桃花桃仁提取物，然后加入到水中，搅拌，均匀后加入防腐剂和香精，搅拌均匀，即得。

Claims (10)

  1. 桃花桃仁复方提取物在紫外损伤修复和促进能量代谢中的用途。
  2. 桃花桃仁复方提取物作为具有紫外损伤修复和促进能量代谢作用的添加剂在化妆品中的用途。
  3. 如权利要求1或2所述的用途，其特征在于，所述桃花桃仁复方提取物的用量在包含0.00001-100重量％。
  4. 如权利要求1或2所述的用途，其特征在于，所述桃花桃仁复方提取物的用量在包含0.0001-50重量％。
  5. 如权利要求1或2所述的用途，其特征在于，所述桃花桃仁复方提取物的用量包含0.001-20重量％。
  6. 如权利要求2所述的用途，其特征在于，所述化妆品选自：洁面乳、化妆水、乳液、膏霜、啫喱、面膜、洗发水、沐浴露、护发素。
  7. 如权利要求6所述的用途，其特征在于，所述化妆品包含桃花桃仁复方提取物以及可接受的辅料。
  8. 如权利要求6所述的用途，其特征在于，所述化妆品包含0.00001-100重量％的桃花桃仁复方提取物。
  9. 如权利要求6所述的用途，其特征在于，所述化妆品包含0.0001-50重量％的桃花桃仁复方提取物。
  10. 如权利要求6所述的用途，其特征在于，所述化妆品包含0.001-20重量％的桃花桃仁复方提取物。
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