WO2015066631A3 - Cell culture medium and bioprocess optimization - Google Patents

Cell culture medium and bioprocess optimization Download PDF

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Publication number
WO2015066631A3
WO2015066631A3 PCT/US2014/063725 US2014063725W WO2015066631A3 WO 2015066631 A3 WO2015066631 A3 WO 2015066631A3 US 2014063725 W US2014063725 W US 2014063725W WO 2015066631 A3 WO2015066631 A3 WO 2015066631A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
chemically defined
cell culture
cdm
cell growth
Prior art date
Application number
PCT/US2014/063725
Other languages
French (fr)
Other versions
WO2015066631A2 (en
WO2015066631A9 (en
Inventor
Jeremiah ZARTMAN
Miranda BURNETTE
Jun Li
Teresa BRITO-ROBINSON
Original Assignee
University Of Notre Dame Du Lac
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Notre Dame Du Lac filed Critical University Of Notre Dame Du Lac
Priority to US15/033,531 priority Critical patent/US20160251614A1/en
Publication of WO2015066631A2 publication Critical patent/WO2015066631A2/en
Publication of WO2015066631A9 publication Critical patent/WO2015066631A9/en
Publication of WO2015066631A3 publication Critical patent/WO2015066631A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0601—Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5085—Supracellular entities, e.g. tissue, organisms of invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a chemically defined cell culture media and methods of using the media. The invention also provides an inverse screen to identify small molecules and synergies stimulating proliferation in a chemically defined medium. In this chemical- genetics approach, a compound-protein interaction database is used to systematically score genetic targets on a screen-wide scale to extract further information about cell growth. Validated factors were investigated for their ability to maintain cell growth over multiple passages in the chemically defined medium (CDM). Polyamines were identified as important components that enables the CDM to support the long-term maintenance of C1.8 cells and Kc cells (such as Kc167 cells). Our cumulative target scoring approach improves on traditional chemical-genetics methods and is extensible to biological processes in other species.
PCT/US2014/063725 2013-11-01 2014-11-03 Cell culture medium and bioprocess optimization WO2015066631A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/033,531 US20160251614A1 (en) 2013-11-01 2014-11-03 Cell culture medium and bioprocess optimization

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361898852P 2013-11-01 2013-11-01
US61/898,852 2013-11-01
US201462027980P 2014-07-23 2014-07-23
US62/027,980 2014-07-23

Publications (3)

Publication Number Publication Date
WO2015066631A2 WO2015066631A2 (en) 2015-05-07
WO2015066631A9 WO2015066631A9 (en) 2015-07-02
WO2015066631A3 true WO2015066631A3 (en) 2015-11-05

Family

ID=53005407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/063725 WO2015066631A2 (en) 2013-11-01 2014-11-03 Cell culture medium and bioprocess optimization

Country Status (1)

Country Link
WO (1) WO2015066631A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3420072A4 (en) * 2016-02-22 2019-08-28 Agency for Science, Technology and Research Cell culture medium
FR3074018B1 (en) 2017-11-27 2020-09-11 Lab Francais Du Fractionnement THERAPEUTIC-AIMED CELL CRYOPRESERVATION PROCESS

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4205126A (en) * 1978-01-01 1980-05-27 Cartaya Oscar A Serum-free cell culture media
WO1992005247A1 (en) * 1990-09-25 1992-04-02 Smithkline Beecham Corporation Media for culture of insect cells
WO1999035242A1 (en) * 1998-01-12 1999-07-15 Betagene, Inc. Media for neuroendocrine cells
CN101864393A (en) * 2009-05-06 2010-10-20 中国人民解放军军事医学科学院生物工程研究所 Serum-free culture medium without animal origin components for culturing Vero cell micro-carrier
US20130236973A1 (en) * 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined Media for Expansion and Maintenance of Pluripotent Stem Cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4205126A (en) * 1978-01-01 1980-05-27 Cartaya Oscar A Serum-free cell culture media
WO1992005247A1 (en) * 1990-09-25 1992-04-02 Smithkline Beecham Corporation Media for culture of insect cells
WO1999035242A1 (en) * 1998-01-12 1999-07-15 Betagene, Inc. Media for neuroendocrine cells
CN101864393A (en) * 2009-05-06 2010-10-20 中国人民解放军军事医学科学院生物工程研究所 Serum-free culture medium without animal origin components for culturing Vero cell micro-carrier
US20130236973A1 (en) * 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined Media for Expansion and Maintenance of Pluripotent Stem Cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BACH, EA ET AL.: "A Sensitized Genetic Screen To Identify Novel Regulators And Components Of The Drosophila Janus Kinase/Signal Transducer And Activator of Transcription Pathway.", GENETICS, vol. 165, no. 3, November 2003 (2003-11-01), pages 1149 - 1166, XP055235152 *
BURNETTE, M ET AL.: "An Inverse Small Molecule Screen To Design A Chemically Defined Medium Supporting Long-Term Growth Of Drosophila Cell Lines.", MOL BIOSYST., vol. 10, no. 10, October 2014 (2014-10-01), pages 2713 - 2723, XP055235153 *
PANDEY, UB ET AL.: "Human Disease Models In Drosophila Melanogaster And The Role Of The Fly In Therapeutic Drug Discovery.", PHARMACOL REV., vol. 63, no. 2, June 2011 (2011-06-01), pages 411 - 436, XP055235150 *

Also Published As

Publication number Publication date
WO2015066631A2 (en) 2015-05-07
WO2015066631A9 (en) 2015-07-02

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