WO2015062497A1 - 一种定量存贮加注器 - Google Patents

一种定量存贮加注器 Download PDF

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Publication number
WO2015062497A1
WO2015062497A1 PCT/CN2014/089774 CN2014089774W WO2015062497A1 WO 2015062497 A1 WO2015062497 A1 WO 2015062497A1 CN 2014089774 W CN2014089774 W CN 2014089774W WO 2015062497 A1 WO2015062497 A1 WO 2015062497A1
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WO
WIPO (PCT)
Prior art keywords
reagent
piston
dispenser according
quantitative storage
wall
Prior art date
Application number
PCT/CN2014/089774
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English (en)
French (fr)
Inventor
郝书顺
Original Assignee
石家庄禾柏生物技术股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from CN201320674793.6U external-priority patent/CN203540572U/zh
Priority claimed from CN201320674318.9U external-priority patent/CN203551578U/zh
Priority claimed from CN201420208844.0U external-priority patent/CN203971970U/zh
Priority claimed from CN201420208279.8U external-priority patent/CN203803521U/zh
Application filed by 石家庄禾柏生物技术股份有限公司 filed Critical 石家庄禾柏生物技术股份有限公司
Publication of WO2015062497A1 publication Critical patent/WO2015062497A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/047—Additional chamber, reservoir
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs

Definitions

  • the present invention relates to a quantitative storage dispenser suitable for use in quantitative addition of reagents in the biological, chemical, and medical fields.
  • Quantifiers are widely used in rapid detection and dosing of biological, chemical, and medical fields, and are often used in conjunction with sampling heads and cuvettes.
  • the sampling head collects the sample and then adds it to the reaction cup.
  • the quantitative storage injector adds the reaction reagent stored therein to the reaction cup, and the sample and the reaction reagent complete the reaction in the reaction cup and finally detect the content of the specific component in the sample. .
  • the precise addition of the sample and reagents during this process is a necessary prerequisite for the accuracy of the experimental results.
  • Chinese Patent No. CN101581729A discloses a liquid sample processing device, wherein a capillary tube is used for collecting a sample and inserting it into a cavity pre-filled with a reagent to realize integration of sample collection and reagent loading.
  • Chinese patent CN101835423A discloses a sampling and dispensing device, the front end of which is provided with a capillary for sampling, and is inserted into a container preset with a reagent, and cooperates with the piston to realize integration of sample collection and reagent installation. .
  • Chinese patent CN102072949B discloses an integrated quantitative sampling and reagent device, wherein a quantitative capillary at the front end is inserted into a quantitative injector composed of a stent, a reagent cartridge body and a piston to realize integration of sample collection and reagent installation.
  • Chinese patent CN202903768U discloses an improved structure of a quantitative sampling and reagent device, the sampling head is composed of a capillary tube, a strut and a connecting head, wherein the capillary is used for sampling, the strut is used for fixing the capillary, and the connecting head is used for the connector.
  • the quantitative filling device consisting of a sealing ring and a reagent tube realizes the integration of sample collection and reagent loading.
  • the quantitative storage injector of the present invention comprises a fixing column, a sleeve disposed at the rear of the fixing column, and a piston sleeved inside the sleeve, the side of the fixing column is provided with a sealing ring groove, and is fixed a top end of the column is provided with a through hole; a hollow tube is disposed in the sleeve, and a tail end of the hollow tube is provided with a piercing tip, and a central hole of the hollow tube communicates with the through hole;
  • the inside of the plug is provided with a reagent cartridge and a sealing membrane for closing the reagent cartridge; the outer wall of the piston closely fits the inner wall of the sleeve, and the inner wall of the piston closely fits the outer wall of the hollow tube.
  • the seal ring groove includes a plurality of bosses and a groove between the bosses, and the boss is provided with a slit.
  • the gap is dislocated on the boss, or the slit is arranged in a spiral shape.
  • the gap of the quantitative storage injector of the present invention is 90 degrees or 180 degrees at an angle with the plane projection.
  • the fixing column of the quantitative storage injector of the present invention is further provided with a strut, a side of the strut having a through hole is provided with a guiding groove; or the strut is provided with a through hole Diversion window.
  • the front end of the struts of the quantitative storage dispenser of the present invention is further provided with a connecting member.
  • the connecting member of the quantitative storage injector of the present invention is disposed on a side of the front end of the strut away from the through hole.
  • the quantitative storage injector of the present invention is provided with a damper table on the outer wall of the piston.
  • the inner wall of the sleeve of the quantitative storage injector of the present invention is provided with a stop groove corresponding to the damper table.
  • the damper table of the quantitative storage injector of the present invention is an annular boss or an arcuate boss.
  • the metered storage dispenser of the present invention also includes a locking ring for attaching the sleeve to the piston.
  • the quantitative storage injector of the present invention is provided with a locking boss on the outer wall of the piston, and the locking boss is disposed below the damper table and closely fits with the locking ring.
  • the sealing film of the present invention comprises an outer layer and an inner layer, the inner layer being made of the same material as the cuvette; and the outer layer being an aluminum film or a plastic film.
  • the quantitative storage injector of the present invention is provided with a sealing collar at the port of the reagent compartment.
  • the piston of the quantitative storage dispenser of the present invention is an elastic material.
  • the reagent storage bin of the present invention is disposed at the front end of the piston, and the rear end of the piston is provided with a plug.
  • the invention reduces the reagent residue by improving the outflow manner of the reagent in the reagent cartridge, improves the structure of the sealing ring groove, reduces the reagent overflow, improves the internal structure, and controls the flow rate and flow rate of the reagent;
  • the welding method of the reagent cartridge and the sealing film is improved, and the reagent residue caused by the denaturation of the reagent cartridge is reduced; thereby completely discharging the reagent in the reagent chamber to the reaction cup, further increasing the detection accuracy.
  • the capillary of the invention is fixed on the fixed column for collecting the sample and the sample; the reagent chamber is used for storing the reagent and flowing out from the through hole; the capillary and the reagent chamber are not connected, that is, the capillary completes the sampling, and the reagent chamber completes the reagent addition.
  • Sampling and filling reagents separately does not affect the sequence, does not interfere with each other, avoids the advance contact between the sample and the reagent in the injector, can improve the accuracy of the sample and reagent filling, and can achieve the dilution of the sample first and the reaction cup. Add the reagent after mixing the solution evenly.
  • the sleeve, the hollow tube and the reagent cartridge of the invention form a double piston, and the introduction of the reagent is completed, and the first piston moves, that is, the reagent cartridge after the membrane is broken on the piston, and the filling and pressing is completed by the hollow tube; the second piston
  • the movement is an annular cavity formed by the outer wall of the reagent chamber and the inner wall of the sleeve, and is filled and squeezed by the wall thickness (tubular) of the reagent cartridge, so that the gas and the reagent injected into the reagent cartridge box from the reagent cartridge are all quickly pushed from the through hole to the movement. In the reaction cup, the precise filling reagent is reached.
  • the through hole of the present invention is disposed on one side of the strut, which can eliminate the blocking of the ejected reagent by the strut; more preferably, the connecting member of the through hole and the front end of the strut is located on different sides of the strut, further eliminating the spray of the connecting member
  • the blocking of the reagent can make the reagent enter the reaction cup more smoothly without remaining; more preferably, a guide groove or a diversion window is provided on the side of the support near the through hole for the reagent diversion.
  • the struts on the fixed column of the present invention are used to connect to the sampling head, and the sealing ring groove is used to connect with the reaction cup.
  • the gap provided in the sealing ring groove is used for arranging the air bubbles generated by the fixing rod into the reaction cup at the gap, preventing the liquid in the reaction cup from overflowing outside the reaction cup due to the bubble generated by the injecting gas.
  • the slit of the present invention is spirally arranged on the boss of the seal ring groove. Compared with the linear arrangement, the spiral arrangement greatly increases the path of the bubble arrangement, and the path is minimized. The leakage of liquid in the cuvette occurs.
  • the angle between the adjacent slits projected on the horizontal plane is 180°, the effect is optimal, and the manufacturing process and cost are saved.
  • the outer wall of the piston of the present invention is provided with a damper table.
  • the preferred damper table may be an annular boss or an arc-shaped boss.
  • the damper table cooperates with the tail end of the sleeve or the tail end of the locking ring to avoid the piston and the sleeve. Undesirable relative sliding occurs as a simple and effective locking device for the fixation of the piston of the present invention during transport storage.
  • the inner wall of the sleeve of the present invention is provided with a stop groove, and the preferred stop groove may be an annular groove; 1 to 3 stop grooves are longitudinally arranged on the inner wall of the sleeve to prevent the piston from slipping off instantaneously with respect to the sleeve, by controlling the piston Movement can control the flow rate and flow rate of the reagents in it to meet the needs of different tests.
  • the sealing chamber of the reagent cartridge of the present invention is provided with a sealing convex ring, and the sealing film is in contact with the sealing convex ring when the sealing film is processed, and is welded to the reagent cartridge port, and the sealing convex ring and the sealing film and the reagent cartridge are welded after welding.
  • the port is integrated into the whole body and welded tightly; and the deformation of the reagent cartridge port and the hot press is prevented from overheating and deformation, which affects the cooperation with the sampler reagent cartridge body; furthermore, the sealing convex ring acts when the film is pressed. The effect of the sealing film is tightened, so that it is flatter after welding.
  • Figure 1 is a view showing the state of use of the quantitative storage injector of the present invention
  • Figure 2 is an assembly view of each part of the quantitative storage injector of the present invention after assembling the sampling head;
  • Figure 3 is a cross-sectional view showing the fixing post and the sleeve of the quantitative storage injector of the present invention
  • Figure 4 is a cross-sectional view of the locking ring of the quantitative storage dispenser of the present invention.
  • Figure 5 is a schematic view showing the structure of a piston of a quantitative storage injector of the present invention.
  • Figure 6 is a schematic view showing the structure of the piston of the quantitative storage injector of the present invention after assembling the locking ring;
  • Figure 7 is a cross-sectional view showing the piston of the quantitative storage dispenser of the present invention when the sealing film is not attached;
  • Figure 8 is a cross-sectional view showing the sealing film of the quantitative storage injector of the present invention.
  • Figure 8 is a schematic view showing the structure of a fixing column and a sleeve of the quantitative storage injector of Embodiment 2;
  • Figure 10 is a schematic view showing the structure of a fixing column and a sleeve of the quantitative storage injector of Embodiment 3;
  • Figure 11 is a rear elevational view of the fixed column and sleeve of the quantitative storage dispenser of Example 3.
  • the present invention can also form an integrated quantitative sampling and reagent device by means of a fixed connection with the sampling head.
  • the manner of fixed connection that can be used includes, but is not limited to, integral injection molding, plugging or screwing.
  • the sampling head therein includes, but is not limited to, a commercially available quantitative sampler or a quantitative capillary disclosed in the patent CN201120293298.1.
  • the present invention can be used in conjunction with a cuvette including, but not limited to, a commercially available cuvette or a cuvette disclosed in the patent CN201120293301.X.
  • the quantitative storage injector of the present invention comprises a fixing post 1, a sleeve 3 disposed at the rear of the fixing post 1, and a piston 4 sleeved inside the sleeve 3.
  • the side of the fixed column 1 is provided with a seal ring groove 2.
  • the present invention is used after assembling the sampling head 8 in use.
  • the sampling head 8 is connected to the connecting member 1-2 at the front end of the rod 1-1 of the present invention, and the sampling head 8 is used to perform accurate sampling; the sampled device is inserted into the reaction cup 7 in which the diluent is installed, The two are connected by a seal ring groove 2 provided on the side of the fixed column 1; the reaction cup 7 is shaken to mix the sample in the sampling head 8 and the diluent in the reaction cup 7. Pressing the piston 4 hardly causes the piston 4 to break through the restriction of the locking boss 4-4, and relatively slides relative to the sleeve 3. At this time, the piercing thimble 3-2 provided at the tail of the hollow tube 3-1 is pierced.
  • the sealing film 4-2 of the reagent chamber 4-1 of the front end of the piston 4 continues to press the piston 4 to pass the reagent stored in the reagent chamber 4-1 through the hollow tube 3-1 and the through hole 2 provided at the top end of the fixed column 1. -1 flows into the reaction cup 7. Since the piston 4 has an elastic structure, a hard plug 6 is provided at the rear end of the piston 4 for the convenience of pressing.
  • the support rod 1-1, the fixed column 1, the seal ring groove 2, the sleeve 3, the hollow tube 3-1, and the piercing thimble 3-2 may be integrally injection molded or may be a split structure.
  • the side of the fixed column 1 of the quantitative storage injector of the present invention is provided with a seal ring groove 2, and the top end of the fixed column 1 is provided with a through hole 2-1; 3 is provided with a hollow tube 3-1, the end of the hollow tube 3-1 is provided with a piercing tip 3-2, and the central hole of the hollow tube 3-1 is connected with the through hole 2-1 .
  • the fixing post 1 is further provided with a strut 1-1, and a side of the strut 1-1 near the through hole 2-1 is provided with a guiding groove 1-3.
  • the front end of the strut 1-1 is further provided with a connecting member 1-2 for connecting the sampling head 8; the connecting member 1-2 is disposed at the front end of the strut 1-1 away from the through hole 2 - One side of 1.
  • the through hole 2-1 of the present invention is disposed on the side of the strut 1-1, which can eliminate the blocking of the ejected reagent by the strut 1-1; more preferably, the through hole 2-1 and the connecting member 1-2 are located
  • the different sides of the rod 1-1 further eliminate the blocking of the ejected reagent by the connecting member 1-2, so that the reagent can enter the reaction cup more smoothly without remaining; when the root of the connecting member 1-2 reaches the bracket body 1
  • the distance from the top is 1 to 3 mm, which prevents the reagent from being injected into the sampling head after being ejected from the through hole.
  • the support rod 1-1 of the present invention is further provided with a flow guiding groove 1-3 for guiding and guiding the reaction
  • the inner wall of the sleeve 3 of the present invention is in close contact with the outer wall of the piston 4, and the outer wall of the hollow tube 3-1 is in close contact with the inner wall of the piston 4.
  • the end of the hollow tube 3-1 inside the sleeve 3 is provided with a piercing tip 3-2 for piercing the sealing film 4-2 of the piston reagent chamber 4-1 matched thereto, once the sealing film 4-2 After the puncturing, as the piston 4 moves inward, the gas in the sleeve 3 is pressed into the piston reagent chamber 4-1, and the reaction reagent therein is quickly ejected through the through hole 2-1, and the liquid is quickly discharged without residue.
  • the inner wall of the sleeve 3 of the present invention is provided with a stop groove 3-3 corresponding to the damper table 4-3 provided on the outer wall of the piston 4.
  • the 1-3 stop grooves 3-3 are longitudinally arranged on the sleeve 3
  • the inner wall avoids the instantaneous sliding of the piston 4 relative to the sleeve 3.
  • the locking ring 5 can function as a seal and a fixing.
  • the locking ring 5 can be provided in a different color to distinguish the type of reagent in the reagent cartridge 4-1.
  • the outer side of the sleeve 3 is provided with a protrusion 3-5 that cooperates with the locking ring 5, and a fixing groove 5-1 is provided at a corresponding position inside the locking ring for locking
  • the assembly of the ring 5 is fixed; at the same time, the anti-dropping table 3-4 can be arranged on the outer side of the sleeve 3, and the height of the anti-offing table 3-4 is at least consistent with the thickness of the locking ring 5, for preventing the protrusion 3 during assembly. 5 Cannot be installed properly after slipping off the fixing groove 5-1.
  • the piston 4 of the quantitative storage injector of the present invention is provided with a reagent cartridge 4-1 and a sealing membrane 4-2 for closing the reagent cartridge 4-1; the outer wall of the piston 4 is disposed.
  • a damper table 4-3 there is a damper table 4-3; the damper table 4-3 is an annular boss or an arcuate boss.
  • the outer wall of the piston 4 is further provided with a locking boss 4-4, and the locking boss 4-4 is disposed on the damping table 4-3.
  • the lower portion and the locking ring 5 are tightly fitted to avoid undesired relative sliding between the piston 4 and the sleeve 3. It is a simple and effective locking device for fixing the piston in the transportation and storage of the present invention.
  • the piston 4 of the present invention is made of an elastic material; the reagent chamber 4-1 is disposed at the front end of the piston 4, and the rear end of the piston 4 is provided with a plug 6.
  • the plug 6 can increase the contact area with the user's finger, which is advantageous for pushing the piston body 1; and the material can be further improved by selecting the material, and the movable connection is connected to the rear end of the piston body 1 and needs to be installed. It can be removed when the ring 13 is locked.
  • a seal collar 4-1-1 is disposed at the port of the reagent cartridge 4-1.
  • the function of the sealing collar 4-1-1 is to seal the sealing film 4-2 with the sealing collar 4-1-1 and solder it to the reagent cartridge port, and seal the convex ring after welding. 1 is integrated with the sealing film and the reagent cartridge port as a whole, and the welding is tight; and the deformation of the reagent cartridge port and the hot press is prevented from being overheated and deformed, which affects the cooperation with the sampler reagent cartridge body;
  • the sealing ring 41-1 of the film acts to tension the sealing film and is therefore flatter after welding.
  • the cross section of the sealing collar 4-1-1 may be a semicircular, semi-elliptical, isosceles triangle or an isosceles trapezoid, wherein an isosceles triangle or an isosceles trapezoid is preferred for the sealing film.
  • the tensioning effect is optimal, and the flatness of the sealing film after welding the sealing film is good, and further preferably an isosceles triangle.
  • the front end of the reagent cartridge 4-1 is sealed with a sealing film 4-2 for sealing the reagent therein, and the sealing film 4-2 includes an outer layer 4-2-1 and an inner layer 4-2-2, the inner layer
  • the material of 4-2-2 is the same as the material of the cuvette 7; the outer layer 4-2-1 is an aluminum film or a plastic film.
  • the seal ring groove 2 of the present invention is provided with a slit 2-4 for arranging the bubbles generated by the insertion of the fixing post 1 into the reaction cup at the gap 2-4 to prevent the liquid in the reaction cup.
  • the bubble is generated by the intrusion of gas and overflows outside the reaction cup.
  • the slits 2-4 are linearly arranged on the boss 2-2 of the seal ring groove.
  • the seal ring groove 2 of the present invention is provided with a slit 2-4 for arranging the bubbles generated by the insertion of the fixing post 1 into the reaction cup at the gap 2-4 to prevent the reaction cup.
  • the liquid overflows outside the cuvette due to bubbles generated by the intrusion of gas.
  • the slits 2-4 are spirally arranged on the boss 2-2 of the seal ring groove, and the spiral arrangement greatly increases the bubble as compared with the linear arrangement in the second embodiment. The arrangement of the paths minimizes the occurrence of liquid leakage in the cuvette.

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Abstract

一种定量存贮加注器，包括固定柱（1）、设置于固定柱（1）后部的套筒（3）以及套置于套筒（3）内侧的活塞（4）。固定柱（1）的侧边设置有密封环槽（2），固定柱（1）的顶端设置有通孔（2-1）。套筒（3）内设置有中空管（3-1），中空管（3-1）的尾端设置有刺破顶尖（3-2），中空管（3-1）的中心孔与通孔（2-1）相连通。活塞（4）内设置有试剂仓（4-1）及封闭试剂仓（4-1）的密封膜（4-2）。活塞（4）的外壁与套筒（3）的内壁紧密贴合，活塞（4）的内壁与中空管（3-1）的外壁紧密贴合。该加注器适用于生物、化学、医学领域的定量加试剂。为提高加注器加试剂的精确度，分别通过改进试剂仓中试剂的流出方式，减少试剂残留，设置密封环槽，减少试剂外溢，并可控制试剂流速和流量。改进试剂仓与密封膜的焊接方式，减少试剂仓变形导致的试剂残留。从而实现了将试剂仓中的试剂完全导出至反应杯，进一步增加了检测准确性。

Description

一种定量存贮加注器 技术领域
本发明涉及一种定量存贮加注器，其适用于生物、化学、医学领域的定量加试剂。
背景技术
定量加注器在生物、化学、医学领域的快速检测、定量加试剂中有着广泛的应用，其通常与采样头和反应杯配合使用。采样头进行采集样品后加入反应杯，定量存贮加注器则将其内存贮的反应试剂加入反应杯，使样品与反应试剂在反应杯中完成反应并最终检测样品中特定组分的含量。在该过程中样品和反应试剂的精确加入是实验结果精确的必要前提。
目前，现有技术给出了将采样头与定量加注器一体化的技术方案：
如中国专利CN101581729A公开了一种液体样本处理装置，其毛细管用于采集样本并插装入预装有试剂的腔体中，实现样本采集与试剂加装的一体化。
中国专利CN101835423A公开了一种取样和分配装置，其取样器的前端设置有用于取样的毛细管，并通过插装入预置有试剂的容器，并与活塞配合实现样本采集与试剂加装的一体化。
中国专利CN102072949B公开了一种一体化定量采样加试剂装置，其前端的定量毛细管插接至由支架、试剂盒体和活塞组成的定量加注器上，实现样本采集与试剂加装的一体化。
中国专利CN202903768U公开了一种定量采样加试剂装置的改良结构，其采样头由毛细管、支杆和连接头组成，其中毛细管用于采样，支杆用于固定毛细管，连接头用于与由连接器、密封圈和试剂管组成的定量加注器，实现样本采集与试剂加装的一体化。
但是现有技术只是针对如何使采样和加试剂装置一体化从而方便检测进行了摸索与改进，但是尚未提出如何进一步提高定量存贮加试剂装置的精确度的方案。
发明内容
本发明的目的在于提供一种有效减少试剂残留，及控制试剂外溢从而增加加注精确度的定量加注器。
为了解决上述技术问题，本发明采用的技术方案如下：
本发明的定量存贮加注器包括固定柱、设置于所述固定柱后部的套筒以及套置于所述套筒内侧的活塞，所述固定柱的侧边设置有密封环槽，固定柱的顶端设置有通孔；所述套筒内设置有中空管，所述中空管的尾端设置有刺破顶尖，中空管的中心孔与所述通孔相连通；所述活 塞内设置有试剂仓及封闭试剂仓的密封膜；所述活塞的外壁与所述套筒的内壁紧密贴合，活塞的内壁与中空管的外壁紧密贴合。
本发明的定量存贮加注器所述密封环槽包括若干个凸台及所述凸台之间的凹槽，所述凸台上设置有豁口。
本发明的定量存贮加注器所述豁口错位设置于所述凸台上，或所述豁口呈螺旋状排列。
本发明的定量存贮加注器所述豁口在随平面投影的夹角为90度或为180度。
本发明的定量存贮加注器所述固定柱上进一步设置有支杆，所述支杆上近通孔的一侧设置有导流槽；或者所述支杆上设置有使通孔暴露的导流窗。
本发明的定量存贮加注器所述支杆的前端进一步设置有连接部件。
本发明的定量存贮加注器所述连接部件设置于支杆前端远离通孔的一侧。
本发明的定量存贮加注器所述活塞的外壁上设置有阻尼台。
本发明的定量存贮加注器所述套筒内壁上设置有与所述阻尼台相对应的中止槽。
本发明的定量存贮加注器所述阻尼台为环状凸台或弧状凸台。
本发明的定量存贮加注器还包括用于连接套筒和活塞的锁紧环。
本发明的定量存贮加注器所述活塞的外壁上设置有锁紧凸台，所述锁紧凸台设置于阻尼台的下方并与锁紧环紧密配合。
本发明的定量存贮加注器所述密封膜包括外层和内层，所述内层的材质与反应杯的材质相同；所述外层为铝膜或塑料膜。
本发明的定量存贮加注器所述试剂仓的端口处设置有密封凸环。
本发明的定量存贮加注器所述活塞为弹性材质。
本发明的定量存贮加注器所述试剂仓设置于所述活塞的前端，所述活塞的后端设置有堵头。
本发明采用上述技术方案所产生的技术效果如下：
本发明为了提高加注器加试剂的精确度，分别通过改进试剂仓中试剂的流出方式，减少试剂残留；改进密封环槽的结构，减少试剂外溢；改进内部结构，控制试剂流速和流量；以及改进试剂仓与密封膜的焊接方式，减少试剂仓变性导致的试剂残留；从而实现将试剂仓中的试剂完全导出至反应杯，进一步增加了检测的准确性。
本发明的毛细管固定于固定柱上，用于采集样品及样品的流出；试剂仓用于存储试剂，并从通孔中流出；毛细管和试剂仓不连通，即毛细管完成取样，试剂仓完成试剂加入， 分别取样和加注试剂，不影响顺序，互不干扰，避免了样品与试剂在加注器内提前接触，可以提高样品及试剂加注的精确性，同时可以实现样品先与反应杯中的稀释液混合均匀后再加入试剂。
本发明的套筒、中空管与试剂仓构成双活塞，完成将试剂的推出，第一个活塞运动即活塞上破膜后的试剂仓，由中空管完成填充挤压；第二个活塞运动是试剂仓外壁与套筒内壁构成的环状腔体，由试剂仓壁厚(管状)填充挤压，这样将由试剂仓注入到试剂仓盒体内的气体以及试剂全部快速从通孔推入到反应杯中，达到精确加注试剂。
本发明的通孔设置于支杆的一侧，可以消除支杆对于喷出的试剂的阻挡；更优选的通孔与支杆前端的连接部件位于支杆的不同侧，进一步消除连接部件对喷出的试剂的阻挡，可以使试剂更顺畅的进入反应杯体，而不会残留；更优选的，在支杆上近通孔的一侧设置有导流槽或导流窗，用于试剂的导流。
本发明的固定柱上的支杆用于与采样头相连接，密封环槽用于与反应杯相连接。密封环槽上设置的豁口用于使固定杆***反应杯中所产生的气泡排列于豁口处，防止反应杯中的液体由于压入气体产生气泡而溢出于反应杯外侧。作为优选的方案，本发明的豁口呈螺旋状排列于所述密封环槽的凸台上，与直线型的排列方式相比，螺旋状排列大大增加了气泡排列的路径，最大程度的减小了反应杯中的液体泄漏的情况发生。进一步的作为优选的方案，相邻的所述豁口在水平面投影的夹角为180°，效果最优并且节约制作工艺和成本。
本发明的活塞外壁上设置有阻尼台，优选的阻尼台可以是环状凸台或弧状凸台，阻尼台与套筒的尾端或锁紧环的尾端相配合，避免活塞与套筒之间发生不期望的相对滑动，是一种简单有效的锁紧装置，用于本发明在运输贮存中的活塞的固定。本发明的套筒内壁设置有中止槽，优选的中止槽可以是环形凹槽；1～3条中止槽纵向排列于所述套筒的内壁，避免活塞相对于套筒瞬间滑落，通过控制活塞的运动可以控制其中的试剂的流速和流量，满足不同试验的需要。
本发明的试剂仓的端口处设置有密封凸环，其作用在于进行封膜处理时，密封膜与密封凸环相接触，并焊接于试剂仓端口，焊接后密封凸环与密封膜及试剂仓端口相融合为整体，焊接紧密；且避免了试剂仓端口与热压机直接接触后过热而发生变形，影响其与采样器试剂盒体的配合；再者，在压膜时密封凸环起到了拉紧密封膜的作用，因此焊接后更平整。
附图说明
图1为本发明定量存贮加注器的使用状态图；
图2为本发明定量存贮加注器装配采样头后各部分的装配图；
图3为本发明定量存贮加注器的固定柱及套筒的剖视图；
图4为本发明定量存贮加注器的锁紧环的剖视图；
图5为本发明定量存贮加注器的活塞的结构示意图；
图6为本发明定量存贮加注器的活塞装配锁紧环后的结构示意图；
图7为本发明定量存贮加注器的活塞未贴附密封膜时的剖视图；
图8为本发明定量存贮加注器密封膜的剖视图；
图8为实施例2的定量存贮加注器的固定柱及套筒结构示意图；
图10为实施例3的定量存贮加注器的固定柱及套筒结构示意图；
图11为实施例3的定量存贮加注器的固定柱及套筒后视示意图。
在附图中，1固定柱、1-1支杆、1-2连接部件、1-3导流槽、2密封环槽、2-1通孔、2-2凸台、2-3凹槽、2-4豁口、3套筒、3-1中空管、3-2刺破顶尖、3-3中止槽、3-4防脱台、3-5凸起、4活塞、4-1试剂仓、4-1-1密封凸环、4-2密封膜、4-2-1外层、4-2-2内层、4-3阻尼台、4-4锁紧凸台、5锁紧环、5-1固定槽、6堵头、7反应杯、8采样头。
具体实施方式
可以选择的，本发明也可以通过与采样头固定连接的方式构成一体式定量采样加试剂装置，可采用的固定连接的方式包括但不限于一体式注塑成型、插接或螺旋连接的方式。其中的采样头包括但不限于市售的定量采样器或专利CN201120293298.1所公开的定量毛细管。本发明可以与反应杯配合使用，其中的反应杯包括但不限于市售的反应杯或专利CN201120293301.X所公开的反应杯。
实施例1
如图1～3所示，本发明定量存贮加注器包括固定柱1、设置于所述固定柱1后部的套筒3以及套置于所述套筒3内侧的活塞4，所述固定柱1的侧边设置有密封环槽2。本发明在使用时装配采样头8后使用。在使用时，将采样头8与本发明支杆1-1前端的连接部件1-2相连接，使用采样头8完成精确采样；将采样后的装置***装好稀释液的反应杯7中，二者通过设置于固定柱1侧方的密封环槽2相连接；摇动反应杯7，使采样头8中的样品和反应杯7中的稀释液混匀。用力压下活塞4，使活塞4突破锁紧凸台4-4的限制，相对于套筒3发生相对滑动，此时中空管3-1尾部设置的刺破顶针3-2刺破设置于活塞4前端试剂仓4-1的密封膜4-2，当继续按下活塞4使储存于试剂仓4-1中的试剂通过中空管3-1及设置于固定柱1顶端的通孔2-1流入反应杯7中。由于活塞4为弹性结构，为方便按压，故在活塞4的尾端设置硬质堵头6。
其中支杆1-1、固定柱1、密封环槽2、套筒3及中空管3-1、刺破顶针3-2可以为一体注塑成型，也可以为分体式构造。
如图2、3和4所示，本发明定量存贮加注器所述固定柱1的侧边设置有密封环槽2，固定柱1的顶端设置有通孔2-1；所述套筒3内设置有中空管3-1，所述中空管3-1的尾端设置有刺破顶尖3-2，中空管3-1的中心孔与所述通孔2-1相连通。所述固定柱1上进一步设置有支杆1-1，所述支杆1-1上近通孔2-1的一侧设置有导流槽1-3。所述支杆1-1的前端进一步设置有连接部件1-2，连接部件1-2用于连接采样头8；所述连接部件1-2设置于支杆1-1前端远离通孔2-1的一侧。本发明的通孔2-1设置于支杆1-1的侧方，可以消除支杆1-1对于喷出的试剂的阻挡；更优选的通孔2-1与连接部件1-2位于支杆1-1的不同侧，进一步消除连接部件1-2对喷出的试剂的阻挡，可以使试剂更顺畅的进入反应杯体，而不会残留；当连接部件1-2的根部到支架体1顶端的距离为1～3mm，可以避免试剂从通孔中喷出后灌入采样头。本发明所述支杆1-1上进一步设置有导流槽1-3，用于反应试剂从通孔2-1喷出后的导向和导流，减少液体飞溅。
本发明所述套筒3的内壁与活塞4的外壁紧密贴合，中空管3-1的外壁与活塞4的内壁紧密贴合。套筒3内部的中空管3-1尾端设置有刺破顶尖3-2，用于刺破与之相匹配的活塞试剂仓4-1的密封膜4-2，一旦密封膜4-2刺破后，随着活塞4向内运动，将套筒3中的气体压入活塞试剂仓4-1，将其中的反应试剂快速通过通孔2-1喷出，液体导出迅速，无残留。
本发明所述套筒3内壁上设置有与活塞4外壁上设置的阻尼台4-3相对应的中止槽3-3。1～3条中止槽3-3纵向排列于所述套筒3的内壁，避免活塞4相对于套筒3瞬间滑落，通过控制活塞4的运动可以控制其中的试剂的流速与流量，满足不同试验的需要。
本发明的套筒3与活塞4借助锁紧环5连接时，锁紧环5可以起到密封和固定的作用。锁紧环5可以设置为不同的颜色，用以区分试剂仓4-1中试剂的种类。当使用锁紧环5时，所述套筒3的外侧设置有与锁紧环5配合的凸起3-5，同时在锁紧环内侧相应位置设置有固定槽5-1，用于锁紧环5的装配固定；同时可以在套筒3的外侧设置防脱台3-4，防脱台3-4的高度至少与锁紧环5的厚度一致，用于防止在装配时凸起3-5与固定槽5-1滑脱后导致的无法正常安装。
如图5～8所示，本发明定量存贮加注器所述活塞4内设置有试剂仓4-1及封闭试剂仓4-1的密封膜4-2；所述活塞4的外壁上设置有阻尼台4-3；所述阻尼台4-3为环状凸台或弧状凸台。所述活塞4的外壁上还设置有锁紧凸台4-4，所述锁紧凸台4-4设置于阻尼台4-3 的下方并与锁紧环5紧密配合，避免活塞4与套筒3之间发生不期望的相对滑动，是一种简单有效的锁紧装置，用于本发明在运输贮存中的活塞的固定。
本发明所述活塞4为弹性材质；所述试剂仓4-1设置于所述活塞4的前端，所述活塞4的后端设置有堵头6。堵头6可增加与使用者手指的接触面积，利于推按活塞体1时的用力；并可通过选择材质，进一步提高使用时的手感，其活动连接于活塞体1的后端，在需要安装锁紧环13时可取下。
所述试剂仓4-1的端口处设置有密封凸环4-1-1。密封凸环4-1-1的作用在于进行封膜处理时，密封膜4-2与密封凸环4-1-1相接触，并焊接于试剂仓端口，焊接后密封凸环4-1-1与密封膜及试剂仓端口相融合为整体，焊接紧密；且避免了试剂仓端口与热压机直接接触后过热而发生变形，影响其与采样器试剂盒体的配合；再者，在压膜时密封凸环4-1-1起到了拉紧密封膜的作用，因此焊接后更平整。为了实现上述功能，密封凸环4-1-1的横截面可以为半圆形、半椭圆形、等腰三角形或等腰梯形，其中优选为等腰三角形或等腰梯形，其对密封膜的拉紧作用最佳，焊接密封膜后密封膜的平整性好，进一步优选为等腰三角形。
所述试剂仓4-1前端密封有密封膜4-2用于封存其中的试剂，所述密封膜4-2包括外层4-2-1和内层4-2-2，所述内层4-2-2的材质与反应杯7的材质相同；所述外层4-2-1为铝膜或塑料膜。
实施例2
如图9所示，本发明的密封环槽2上设置有豁口2-4，其用于使固定柱1***反应杯中所产生的气泡排列于豁口2-4处，防止反应杯中的液体由于压入气体产生气泡而溢出于反应杯外侧。在该实施例中，豁口2-4呈直线排列于密封环槽的凸台2-2上。
实施例3
如图10、11所示，本发明的密封环槽2上设置有豁口2-4，其用于使固定柱1***反应杯中所产生的气泡排列于豁口2-4处，防止反应杯中的液体由于压入气体产生气泡而溢出于反应杯外侧。在该实施例中，豁口2-4呈螺旋状排列于所述密封环槽的凸台2-2上，与实施例2中的呈直线型的排列方式相比，螺旋状排列大大增加了气泡排列的路径，最大程度的减小了反应杯中的液体泄漏的情况发生。当相邻的豁口2-1在水平面投影的夹角为180°，即一个豁口2-1设置于一个密封环槽2的凸台的一侧，则与之相邻的豁口2-1设置于与之相邻的凸台另一侧的相应位置；效果最优并且节约制作工艺和成本。

Claims (16)

  1. 一种定量存贮加注器，其包括固定柱(1)、设置于所述固定柱(1)后部的套筒(3)以及套置于所述套筒(3)内侧的活塞(4)，其特征在于：
    所述固定柱(1)的侧边设置有密封环槽(2)，固定柱(1)的顶端设置有通孔(2-1)；
    所述套筒(3)内设置有中空管(3-1)，所述中空管(3-1)的尾端设置有刺破顶尖(3-2)，中空管(3-1)的中心孔与所述通孔(2-1)相连通；
    所述活塞(4)内设置有试剂仓(4-1)及封闭试剂仓(4-1)的密封膜(4-2)；
    所述活塞(4)的外壁与所述套筒的内壁紧密贴合，活塞(4)的内壁与中空管(3-1)的外壁紧密贴合。
  2. 根据权利要求1所述的一种定量存贮加注器，其特征在于所述密封环槽(2)包括若干个凸台(2-2)及所述凸台(2-2)之间的凹槽(2-3)，所述凸台(2-2)上设置有豁口(2-4)。
  3. 根据权利要求2所述的一种定量存贮加注器，其特征在于所述豁口(2-4)错位设置于所述凸台(2-2)上，或所述豁口(2-4)呈螺旋状排列。
  4. 根据权利要求3所述的一种定量存贮加注器，其特征在于所述豁口(2-4)在随平面投影的夹角为90度或为180度。
  5. 根据权利要求1～4任意一项所述的一种定量存贮加注器，其特征在于所述固定柱(1)上还设置有支杆(1-1)，所述支杆(1-1)上近通孔(2-1)的一侧设置有导流槽(1-3)；
    或者所述支杆(1-1)上设置有使通孔(2-1)暴露的导流窗(1-4)。
  6. 根据权利要求5所述的一种定量存贮加注器，其特征在于所述支杆(1-1)的前端还设置有连接部件(1-2)。
  7. 根据权利要求6所述的一种定量存贮加注器，其特征在于所述连接部件(1-2)设置于支杆(1-1)前端远离通孔(2-1)的一侧。
  8. 根据权利要求1～7的任意一项所述的一种定量存贮加注器，其特征在于所述活塞(4)的外壁上设置有阻尼台(4-3)。
  9. 根据权利要求8所述的一种定量存贮加注器，其特征在于所述套筒(3)内壁上设置有与所述阻尼台(4-3)相对应的中止槽(3-3)。
  10. 根据权利要求8所述一种定量存贮加注器，其特征在于所述阻尼台(4-3)为环状凸台或弧状凸台。
  11. 根据权利要求1～10任意一项所述的一种定量存贮加注器，其特征在于其还包括用于连接套筒(3)和活塞(4)的锁紧环(5)。
  12. 根据权利要求11所述的一种定量存贮加注器，其特征在于所述活塞(4)的外壁上设置 有锁紧凸台(4-4)，所述锁紧凸台(4-4)设置于阻尼台(4-3)的下方并与锁紧环(5)紧密配合。
  13. 根据权利要求1～12任意一项所述的一种定量存贮加注器，其特征在于所述密封膜(4-2)包括外层(4-2-1)和内层(4-2-2)，所述内层(4-2-2)的材质与反应杯(7)的材质相同；所述外层(4-2-1)为铝膜或塑料膜。
  14. 根据权利要求1～12任意一项所述的一种定量存贮加注器，其特征在于所述试剂仓(4-1)的端口处设置有密封凸环(4-1-1)。
  15. 根据权利要求1～12任意一项所述的一种定量存贮加注器，其特征在于所述活塞(4)为弹性材质。
  16. 根据权利要求15所述的一种定量存贮加注器，其特征在于所述试剂仓(4-1)设置于所述活塞(4)的前端，所述活塞(4)的后端设置有堵头(6)。
PCT/CN2014/089774 2013-10-30 2014-10-29 一种定量存贮加注器 WO2015062497A1 (zh)

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CN201320674318.9U CN203551578U (zh) 2013-10-30 2013-10-30 一种定量加注器
CN201320674318.9 2013-10-30
CN201420208844.0 2014-04-28
CN201420208279.8 2014-04-28
CN201420208844.0U CN203971970U (zh) 2014-04-28 2014-04-28 一种定量采样加试剂装置用加注器
CN201420208279.8U CN203803521U (zh) 2014-04-28 2014-04-28 一种定量采样加试剂装置用活塞试剂仓

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