WO2015033507A1 - Cell culture flask and cell culture flask manufacturing method - Google Patents

Cell culture flask and cell culture flask manufacturing method Download PDF

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Publication number
WO2015033507A1
WO2015033507A1 PCT/JP2014/003734 JP2014003734W WO2015033507A1 WO 2015033507 A1 WO2015033507 A1 WO 2015033507A1 JP 2014003734 W JP2014003734 W JP 2014003734W WO 2015033507 A1 WO2015033507 A1 WO 2015033507A1
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WO
WIPO (PCT)
Prior art keywords
peripheral wall
internal
tray
rising peripheral
wall portion
Prior art date
Application number
PCT/JP2014/003734
Other languages
French (fr)
Japanese (ja)
Inventor
福西　賢晃
春男 大久保
新井　進
Original Assignee
住友ベークライト株式会社
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Publication of WO2015033507A1 publication Critical patent/WO2015033507A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports

Definitions

  • the present invention relates to a cell culture flask. More specifically, the present invention relates to a multistage culture flask for further improving the culture efficiency.
  • a cell culture flask having a structure in which a plurality of trays are stacked is provided.
  • the stacked tray members that define the culture chamber are accommodated in an external casing having a flat bottom wall and end walls.
  • the stacked tray members are sealed around each other, and the end of the upper floor tray member is released by being separated from the inner surface of the top surface of the casing, and the liquid is It is distributed to each culture room through a tunnel provided along the end wall.
  • a multistage cell culture system described in Japanese Patent Application Laid-Open No. 2011-528226 includes a cover including an upper plate, a side wall, and a port; an intermediate tray for storing cells and the like having a bottom plate, a lower portion, and a gap region
  • the gap region is formed between the lip bent outward at the inner portion of the intermediate tray bottom plate and the adjacent side wall portion of the intermediate tray bottom plate bent outward, and the lip has a curved edge shape toward the outer side.
  • a base tray for accommodating cells and the like having a bottom plate and side walls.
  • the intermediate tray is disposed between the cover / base tray, and the gap region of the intermediate tray bottom plate is in line with the port on the cover, and the intermediate tray and the base tray are in fluid communication with each other. Direct access is possible to remove and / or add cells and the like placed above.
  • the intermediate trays are plural stacked on top of each other, and the gap regions of the intermediate trays are in line with each other.
  • a multilayer tissue culture container described in Japanese Patent Application Laid-Open No. 2011-24577 includes a base including a base and a bottom including an upwardly extending wall that at least partially forms a boundary of the base. And a top having a downwardly extending wall that at least partially defines a base boundary, a tubular neck defining an opening, and one or more shelves, each shelf at least defining a base and a base boundary A partially extending upwardly extending wall.
  • a wall extending above the first shelf is adjacent to a wall extending below the top, and the first shelf is located between the bottom and the top.
  • the base of each shelf has at least one hole formed in it.
  • the bottom, top, and one or more shelves aggregate to define an enclosed volume for culturing cells.
  • a tubular neck extends from the container and the enclosed volume is accessible by an opening in the tubular neck.
  • the upper end of the uppermost tray member is isolated from the top surface and is not fixed. Therefore, the culture solution stored in the uppermost tray member may leak out of the tray member through a slight gap between the upper end and the top surface due to capillary action.
  • each of the plurality of shelves, the bottom, and the top are all laminated. Therefore, the stacking deviation at the time of manufacture is substantially unavoidable, and individual product differences in external dimensions always occur.
  • An object of the present invention is to provide a cell culture flask in which the culture medium in the uppermost stage of the intermediate tray does not leak out and can eliminate the individual differences in the external dimensions.
  • a cell culture flask includes an outer casing, an inner multi-stage tray, and a lid.
  • the external housing includes an external bottom surface portion, an external rising peripheral wall portion provided on the entire periphery of the external bottom surface portion, and a port provided in a part of the external rising peripheral wall portion.
  • the internal multi-stage tray is formed by stacking a plurality of internal trays including an internal bottom surface portion and an internal rising peripheral wall portion provided on the entire circumference of the internal bottom surface portion, and stacking on the upper end of the internal rising peripheral wall portion and the upper end. The lower surface of the part is welded to each other and accommodated in the external housing.
  • the lid covers both the upper end of the outer rising peripheral wall portion and the upper end of the inner rising peripheral wall portion of the uppermost internal tray in the internal multi-stage tray, and is welded to both upper ends. Furthermore, a communication hole that connects the space in the internal tray and the space in the external housing is formed in the internal rising peripheral wall portion in each stage of the internal multi-stage tray.
  • the meat stealing portion communicating with the communication hole may be formed over the entire stacking direction of the internal tray.
  • a spacer may protrude from the inner peripheral side of the outer rising peripheral wall portion so as to contact the vicinity of the communication hole of the inner rising peripheral wall portion.
  • a holding portion that holds the inner multi-stage tray separated from the outer bottom surface portion at a predetermined height may be provided on the inner peripheral side of the outer rising peripheral wall portion.
  • the holding part is provided so as to be connected to the spacer.
  • the outer bottom surface can also be used as a culture space. Furthermore, in this case, even if the inner tray stacking is displaced, and the inner rising peripheral wall portion of the lowermost internal tray has a portion in contact with the outer rising peripheral wall portion, the culture medium can be smoothly transferred to the culture space. Can be distributed.
  • the length between the external rising peripheral wall portions facing in the longitudinal direction of the external housing is 1.9 of the length between the external rising peripheral wall portions facing in the short direction of the external housing. It may be not less than twice and not more than 2.2 times.
  • the cell culture flask of the present invention can prevent undesired liquid leakage from the inner tray, such a long type shape that is easily affected by inclination is also allowed.
  • a sufficient culture volume can be ensured even if the depth per step is reduced relative to the capacity per step of the internal tray.
  • the thickness of the inner bottom surface portion may be 1.2 mm or more and 2.0 mm or less.
  • the height of the internal rising peripheral wall portion can be reduced relative to the capacity per one stage of the internal tray, so that the number of stacked stages can be increased relative to the depth of the external casing, and the culture efficiency can be increased. Can be further improved.
  • the strength of the entire cell culture flask is guaranteed even if the thickness of the inner bottom surface of the internal multistage tray is relatively small. .
  • the height of the internal rising peripheral wall portion may be 8.5 mm or more and 11.0 mm or less.
  • the external rising peripheral wall portion may include a pair of opposing side walls, a pair of opposing end walls, and an inclined side wall.
  • a port is formed in one of the pair of opposing end walls
  • the inclined side wall extends from at least one of the pair of side walls and is inclined toward the root of the port in plan view from above.
  • the inclined side wall includes a first inclined side wall and a second inclined side wall.
  • the first inclined sidewall extends from the sidewall and is inclined at a smaller angle with respect to the port axis.
  • the second inclined sidewall extends from the first inclined sidewall and is inclined at a larger angle with respect to the port axis.
  • the internal rising peripheral wall portion of the internal multistage tray is formed along the side wall, the other of the end walls (that is, the side where the port is not formed) and the first inclined side wall among the external rising peripheral wall portions. .
  • the culture volume per stage can be increased by the first inclined side wall inclined at a smaller angle.
  • the method for producing a cell culture flask includes a stacking step, a covering step, and a containing step.
  • a plurality of internal trays including an inner bottom surface portion, an inner rising peripheral wall portion provided on the entire circumference of the inner bottom surface portion, and a notch portion in which a part of the upper end of the inner rising peripheral wall portion is notched.
  • the upper end of the inner rising peripheral wall portion and the lower surface of the inner bottom surface portion stacked on the upper end are welded together to obtain an internal multi-stage tray.
  • the covering step the upper end of the inner rising peripheral wall portion of the uppermost inner tray in the inner multi-stage tray is covered with a lid, and the upper end and the lid are welded to obtain the covered inner multi-stage tray.
  • an internal multi-stage tray with a cover is placed inside an external housing including an external rising peripheral wall portion provided on the entire circumference of the external bottom surface portion and a port provided in a part of the external rising peripheral wall portion. Accommodating and welding the upper end of the external rising peripheral wall and the lid to obtain an internal multi-stage tray housing case.
  • FIG. 2 is a sectional view taken along line AA in FIG. 1.
  • FIG. 2 is a sectional view taken along line BB in FIG.
  • FIG. 6 is an enlarged view of a circled portion in FIG. 5.
  • FIG. 2 is a cross-sectional view taken along the line CC of FIG.
  • FIG. 2 is a partially cutaway enlarged view of FIG. 1.
  • FIG. 4 is a schematic diagram showing a process of a method for manufacturing a culture flask 100.
  • FIG. 4 is a schematic diagram showing a process of a method for manufacturing a culture flask 100. It is explanatory drawing which shows the process of the operating method of the culture flask. It is explanatory drawing which shows the process of the operating method of the culture flask. It is explanatory drawing which shows the process of the operating method of the culture flask. It is explanatory drawing which shows the process of the operating method of the culture flask. It is a partially cutaway enlarged view showing another example of the culture flask 100. It is sectional drawing of FIG. It is sectional drawing of FIG. It is sectional drawing of FIG. 4 is a partially enlarged view showing another example of the culture flask 100. FIG. 4 is a partially enlarged view showing another example of the culture flask 100. FIG. 4 is a partially enlarged view showing another example of the culture flask 100. FIG. 4 is a partially enlarged view showing another example of the culture flask 100. FIG. 4 is a partially enlarged view showing another example of the culture fla
  • FIG. 1 is a schematic external perspective view showing an example of a culture flask 100 according to the first embodiment.
  • FIG. 2 is a schematic exploded view of the culture flask 100 of FIG. 3 is a cross-sectional view taken along line AA in FIG. 4 is a cross-sectional view taken along line BB of FIG.
  • FIG. 5 is a schematic external perspective view showing a part of the culture flask 100 of FIG.
  • FIG. 6 is an enlarged view of a circled portion in FIG. 7 is a cross-sectional view taken along the line CC of FIG.
  • FIG. 8 is an enlarged view of a part of FIG.
  • the culture flask 100 includes an external housing 200, an internal multistage tray 400, and a lid 500.
  • the external housing 200 accommodates the internal multistage tray 400.
  • the lid 500 covers the outer casing 200 and the inner multi-stage tray 400.
  • the culture flask 100 includes a first communication portion 450 and a second communication portion 480 provided on the internal multi-stage tray 400.
  • the culture flask 100 includes a cap 290 attached to the outer casing 200.
  • the external housing 200 includes an external bottom surface portion 210 and an external rising peripheral wall portion 220 provided on the entire periphery thereof.
  • the external rising peripheral wall portion 220 includes a pair of end walls 221 and 222 that face the longitudinal direction of the external housing 200 and a pair of side walls 223 and 224 that face the short side direction.
  • One end wall 221 is provided with a port 280.
  • the port 280 protrudes from the end wall 221 toward the outside of the external housing 200, and is formed in a tubular shape that allows the inside and the outside of the external housing 200 to communicate with each other.
  • the axis of the port 280 is parallel to the longitudinal direction of the outer casing 200 and is located at the center between the side walls 223 and 224.
  • a cap 290 is detachably attached to the port 280 as shown in FIGS.
  • the external rising peripheral wall portion 220 further has an inclined wall inclined toward the root of the port 280.
  • the inclined wall is configured to be line-symmetric with respect to the axis of the port 280 in a plan view from the upper part to the lower part.
  • the inclined walls include a pair of first inclined walls 225 and 226 and a pair of second inclined walls 227 and 228.
  • the first inclined walls 225 and 226 are connected to the side walls 223 and 224, respectively.
  • the second inclined walls 227 and 228 are connected to the first inclined walls 225 and 226, respectively, and are both connected to the end wall 221.
  • the second inclined walls 227 and 228 are inclined at an angle larger than the first inclined walls 225 and 226 toward the axis of the port 280 in a plan view from the upper part to the lower part.
  • a plurality of holding portions 230 to be described later project from the inner peripheral surface of the external rising peripheral wall portion 220.
  • the holding unit 230 is provided so as to hold the internal multi-stage tray 400 at a predetermined height position in the outer casing 200.
  • the holding surface 231 that forms the upper end of the holding unit 230 is in contact with the lowermost surface of the internal multi-stage tray 400 (the lower surface of the inner bottom surface portion 410 described later), and the holding surface 231
  • the position is a position separated from the upper surface of the external bottom surface part 210 by a height H.
  • the space enclosed by the holding portion 230 in the outer casing 200 is the outer bottom surface portion 210, the lowermost surface of the internal multi-stage tray 400 (the lower surface of the inner bottom surface portion 410 described later), and the external rising peripheral wall portion 220.
  • m1 is secured.
  • This space m1 secured by the holding unit 230 can be used as a culture space in the culture flask 100 in the same manner as the space inside the internal multi-stage tray 400 (culture spaces m2,..., M6).
  • the space m1 is referred to as a culture space m1.
  • a spacer 250 protrudes in the vicinity of the first communication portion 450 on the inner peripheral surface of the external rising peripheral wall portion 220.
  • the spacer 250 is provided in the first communication portion 450 in the outer casing 200 so as to secure a gap between the outer peripheral surface of the internal multi-stage tray 400 and the inner peripheral surface of the outer casing 200.
  • the spacer 250 protrudes from the inner peripheral surface of the side wall 224 at a height I, and comes into contact with the outer peripheral surface (side wall 324 described later) of the internal multi-stage tray 400.
  • a gap having a width corresponding to the height I of the spacer 250 is secured between the outer peripheral surface (side wall 324) and the inner peripheral surface of the side wall 224 of the external housing 200.
  • the length between the pair of end walls 221 and 222 facing in the longitudinal direction is 210 mm or more and 240 mm or less, for example, 223 mm.
  • the length between the pair of end walls 221 and 222 facing in the longitudinal direction is 1.9 to 2.2 times, for example, 2.0 times the length of the pair of side walls 223 and 224 facing in the short direction. It is.
  • the angle formed by the first inclined walls 225 and 226 and the axis of the port 280 is not less than 45 degrees and not more than 70 degrees, for example, 60 degrees.
  • the angle formed by the second inclined walls 227 and 228 and the end wall 221 is 3 degrees or more and 10 degrees or less, for example, 5 degrees.
  • the internal multi-stage tray 400 is configured by stacking a plurality of the same and the same size as the internal tray 300 shown in FIG. In the present embodiment, five sheets are stacked. As shown in FIG. 5, the internal tray 300 includes an internal bottom surface portion 310 and an internal rising peripheral wall portion 320 provided on the entire periphery thereof. As shown in FIGS. 2 and 4, the inner tray 300 is stacked in such a manner that the upper end 329 of the inner rising peripheral wall portion 320 and the lower surface of the inner bottom surface portion 310 of another inner tray 300 stacked on the upper end 329 are as follows. They are stacked so that they are welded together. Thus, the inner multi-stage tray 400 has culture spaces m2,..., M5 surrounded by the inner trays 300.
  • the upper end 329 of the uppermost internal tray 300 in the internal multi-stage tray 400 is particularly referred to as the upper end 429 of the internal multi-stage tray 400.
  • the inner bottom surface portion 310 of the lowermost inner tray 300 among the inner multi-stage tray 400 is particularly referred to as an inner bottom surface portion 410 of the inner multi-stage tray 400.
  • the internal rising peripheral wall portion 320 includes a pair of end walls 321 and 322 facing in the longitudinal direction, a pair of side walls 323 and 324 facing in the short direction, and a pair of inclined walls 325 and 326. including.
  • the pair of inclined walls 325 and 326 are connected to both the side walls 323 and 324 and the end wall 321.
  • the side wall 324 is provided with a notch 351 at a position closer to the end wall 322 than the end wall 321, specifically, at a position separated from the end wall 322 by a distance l. More specifically, as shown in FIG. 6, the side wall 324 is provided with a notch 351 that is recessed downward from the upper end 329 with a width w. The side wall 324 is further provided with a meat stealing portion 355 that is recessed inwardly from the outer peripheral surface over the entire height h direction of the side wall 324 with the same width w as the notch portion 351.
  • the end wall 321 is provided with a notch 381 that is recessed downward from the upper end 329.
  • the depth of the notch 381 is not particularly limited as long as a desired culture volume can be ensured. Therefore, the same depth as the notch 351 may be sufficient, and a different depth may be sufficient.
  • the end walls 321 and 322 of each stage of the internal tray 300 constituting the internal multistage tray 400 are the end walls 221 of the external casing 200 when the internal multistage tray 400 is accommodated in the external casing 200 (see FIG. 1). , 222 along the surface.
  • the side walls 323 and 324 of the inner multi-stage tray 400 are along the surfaces of the side walls 223 and 224 of the outer casing 200, and the inclined walls 325 and 326 of the inner multi-stage tray 400 are the first inclined walls 225 of the outer casing 200. , 226 along the surface.
  • the length between the pair of end walls 321 and 322 is 200 mm or more and 230 mm or less, for example, 214 mm.
  • the length between the pair of side walls 323 and 324 is 100 mm or more and 115 mm or less, for example, 107 mm.
  • the height h of the side wall 324 is 8.5 mm or more and 11.0 mm or less, for example, 9.0 mm.
  • the thickness of the inner bottom surface portion 310 of the inner tray 300 is 1.2 mm or more and 2.0 mm or less, for example, 1.6 mm.
  • the depth of the inner tray 300 may be 7.0 mm or more and 9.5 mm or less, for example, 7.4 mm.
  • the thickness of the internal rising peripheral wall 320 of the internal tray 300 may be the same as the thickness of the internal bottom surface 310.
  • the distance l from the end wall 322 of the notch 351 is 15% or more and 25% or less, for example 19%, of the length between the pair of end walls 221 and 222 facing the longitudinal direction of the external housing 200.
  • the width w of the notch 351 is 4% or more and 10% or less, for example, 6% of the length between the end walls 221 and 222 of the external housing 200.
  • the depth d of the notch 351 is 10% to 30% of the height h, for example, 17%.
  • the width of the notch 381 is not less than 40% and not more than 80%, for example 60%, of the length between the pair of side walls 223 and 224 facing the short side direction of the outer casing 200.
  • the lid 500 is configured to cover the entire upper opening of the external housing 200. As shown in FIGS. 3 and 4, the lid 500 covers the outer casing 200 and also covers the internal multistage tray 400 accommodated in the outer casing 200. In this case, the lower surface of the lid 500 is welded to both the upper end 229 of the external rising peripheral wall 220 of the external housing 200 and the upper end 429 of the internal multi-stage tray 400.
  • the culture flask 100 has a culture space m6 surrounded by the uppermost internal tray 300 and the lid 500 of the internal multi-stage tray 400. Therefore, the culture flask 100 includes the culture space m1 and the culture spaces m2,...
  • the notch 351 is provided at a position separated from the end wall 322 by a predetermined distance l, and the notch 381 is provided on the end wall 321 (see FIG. 5).
  • the first communication part 450 is disposed sufficiently separated from the second communication part 480.
  • the first communication part 450 can be used for aeration and for distributing the culture solution to each stage.
  • the first communication portion 450 is configured to communicate the internal spaces of all the internal trays 300 that constitute the internal multistage tray 400 with the internal spaces of the external housing 200.
  • a predetermined gap (corresponding to the height I of the spacer 250 in the figure) is secured by the spacer 250 between the inner multi-stage tray 400 and the outer casing 200.
  • a meat stealing portion 355 is provided on the outer peripheral surface (side wall 324) of the internal multi-stage tray. As shown in FIG. 7, the meat stealing portion 355 is provided over the entire stacking direction of the internal tray 300, and therefore, in the first communication portion 450, the gap S between the internal multi-stage tray 400 and the external housing 200 is A wider space is secured by the amount of the meat stealing portion 355. As a result, the culture solution can easily circulate in the sufficiently secured space S.
  • FIG. 8 is a cutaway enlarged view of the vicinity of the first communication portion 450 in FIG.
  • the meat stealing 355 is provided over the entire stacking direction of the internal tray 300 with a width w.
  • a notch 351 is provided in the side wall 324 of each inner tray 300 with a depth d.
  • the second communication portion is provided in the vicinity of the port 280 in the culture flask 100. In the present embodiment, as shown in FIG. 2, it is disposed on the end wall 321 constituting the internal multi-stage tray 400.
  • the second communication portion can be used for aeration and for sending the culture solution to the port 280. Therefore, the second communication unit is configured to communicate between the internal spaces of all the internal trays 300 constituting the internal multistage tray 400 and the internal space of the external housing 200.
  • the notch 381 (see FIG. 5) is provided in the end wall 321 of each internal tray 300 at a depth d.
  • a second communication hole CH2 surrounded by the notch 381 and the inner bottom surface 310 of the inner tray 300 stacked immediately above is formed.
  • the second communication hole CH2 surrounded by the notch 381 of the uppermost internal tray 300 and the lid 500 covering the upper end 429 is also configured.
  • the content liquid can flow through the second communication hole CH ⁇ b> 2 that communicates the internal space of the internal tray 300 and the internal space of the external housing 200.
  • the outer casing 200, the inner multi-stage tray 400, and the lid 500 constituting the culture flask 100 may be made of a thermoplastic resin. Preferably, it is made of polystyrene.
  • the surface of the constituent member of the culture flask 100 may be appropriately treated by those skilled in the art. For example, surface treatment with a biological agent (for example, extracellular matrix, more specifically, at least one component of laminin, fibronectin, and collagen) or a synthetic agent, or hydrophilic treatment such as plasma treatment is performed. It may be.
  • FIG. 9 and 10 are schematic diagrams showing the steps of the method for manufacturing the culture flask 100.
  • a plurality of (five in the figure) internal trays 300 are prepared.
  • the internal tray 300 is stacked.
  • the internal tray 300 is stacked so that the upper end 329 of the internal tray 300 and the lower surface of the internal bottom surface 310 of another internal tray 300 are in contact with each other, and the upper end 329 and the internal bottom surface 310 are in contact with each other.
  • the welded part is sealed in a liquid-tight manner. Examples of the welding method include an ultrasonic welding method.
  • the welded part is sealed liquid-tight. Thereby, culture spaces m2, ..., m5 are formed.
  • the notch 381 of the inner tray 300 is not welded, and the second communication hole CH2 surrounded by the notch 381 and the inner bottom surface 310 immediately above is formed except for the uppermost stage.
  • the first communication hole CH1 is formed in other notch portions 351 (see FIG. 7) (not shown). Thereby, the internal multi-stage tray 400 is obtained.
  • the lid 500 is covered with the upper end 429 of the internal multi-stage tray 400, and the upper end 429 and the lower surface of the lid 500 are welded.
  • the welding method is the same as in the previous step.
  • the welded part is sealed in a liquid-tight manner.
  • the culture space m6 is further formed.
  • the second communication hole CH2 surrounded by the notch 381 and the lid 500 is formed without welding the notch 381 provided at the uppermost stage of the internal multi-stage tray 400.
  • the second communication portion 480 is formed.
  • the other notch 351 not shown
  • the first communication hole CH1 in the uppermost stage is formed, and the first communication part 450 is formed.
  • the inner multi-stage tray 400 with the lid 500 welded is accommodated in the outer casing 200, and the upper end 229 of the outer casing 200 and the lower surface of the lid 500 are welded together. .
  • the welding method is the same as in the previous step.
  • the culture space m1 is further formed.
  • the cap 290 (see FIG. 1) is appropriately attached to the port 280 to obtain the culture flask 100.
  • FIG. 11 to FIG. 13 are explanatory diagrams showing the steps of the method for operating the culture flask 100.
  • the culture solution M is put into the culture flask 100, and as shown in FIG. 11 (I), the end wall 222 is placed downward and allowed to stand.
  • the culture solution M flows through the first communication portion 450 formed on the side wall 324 near the end wall 222, and the culture solution M is distributed to each stage.
  • the standing time may be such that the culture liquid M circulates in the first communication part 450 so that the liquid level becomes the same height in each stage, specifically about 10 seconds.
  • the culture flask 100 is tilted to the side where the first communication portion 450 is not formed (side wall 223 side of the external housing), and the culture solution M is transferred from the first communication portion 450. Isolate.
  • the angle ⁇ at which the culture flask 100 is tilted is, for example, not less than 45 degrees and not more than 70 degrees.
  • the culture flask 100 is placed so that the external bottom surface portion 210 is at the bottom as shown in FIG. knock down.
  • the culture solution M is evenly distributed to each stage of the culture flask.
  • the culture solution M in each internal tray 300 of the internal multi-stage tray 400 is discharged into the external housing 200 through the second communication hole CH2 of the second communication portion 480 (see FIG. 3). Further, the culture medium M travels from the port 280 to the outside through the inner peripheral surface of the first inclined wall 225 (or the first inclined wall 226) and the second inclined wall 227 (or the second inclined wall 228) of the outer casing 200. Discharged.
  • [Other examples] 14 to 23 show another example of the culture flask 100. In other examples, differences from the first embodiment will be mainly described, and descriptions of the same points will be omitted.
  • FIG. 14 is an enlarged view of a cutout in the vicinity of the first communication portion 450a of the culture flask 100a, and corresponds to FIG. 8 of the first embodiment.
  • 15 is a cross-sectional view taken along line Ba in FIG. 14, and
  • FIG. 16 is a cross-sectional view taken along line Ca in FIG. 15 and 16 correspond to FIGS. 4 and 7 of the first embodiment, respectively.
  • the culture flask 100a has a holding portion 230a in the outer casing 200a, but does not have a spacer.
  • the side wall 324a of the internal multi-stage tray 400a has a cutout portion 351a and a meat stealing portion 355a.
  • the cutout portion 351a and the meat stealing portion 355a are provided with a width wa wider than that in the first embodiment.
  • the internal multi-stage tray 400a is manufactured in the same manner as the internal multi-stage tray 400 of the first embodiment.
  • the stacking deviation of the inner tray 300a may occur.
  • the width of the gap between the side wall 324a of the inner multi-stage tray 400a and the side wall 224a of the outer casing 200a is not constant.
  • the side wall 324a of the lowermost internal tray 300a is close enough to come into contact with the side wall 224a of the outer casing 200a. For this reason, in the lowermost internal tray 300a, a sufficient gap is not secured between the side wall 324a and the side wall 224a. In such a case, if there is no other structure that ensures sufficient communication, a flow failure occurs between the side wall 324a and the side wall 224a, and the culture solution flows to the culture space m1 below the inner bottom surface portion 410a. There is a risk of not being easily distributed.
  • the side wall 224a is provided with a meat stealing portion 355a. Therefore, in the first communication portion 450a, a gap Sa (see FIG. 16) is secured between the meat stealing portion 355a and the side wall 224a even in the lowermost internal tray 300a. Therefore, even when there is a stacking deviation as shown in FIG. 15, the culture solution can be easily distributed to the lowest culture space m1 of the culture flask 100a.
  • FIG. 17 is an enlarged view of a cutout in the vicinity of the first communication portion 450b of the culture flask 100b, and corresponds to FIG. 8 of the first embodiment.
  • 18 is a cross-sectional view taken along line Bb in FIG. 17, and
  • FIG. 19 is a cross-sectional view taken along line Cb in FIG. 18 and 19 correspond to FIGS. 4 and 7 of the first embodiment, respectively.
  • the culture flask 100b has a holding part 230b and a spacer 250b in the outer casing 200b.
  • the internal multi-stage tray 400b has a cutout portion 351b, but does not have a meat stealing portion.
  • the internal multi-stage tray 400b may be misaligned as described above.
  • the side wall 324b of the lowermost internal tray 300b is closest to the side wall 224b of the external housing 200b.
  • the spacer 250b protruding from the side wall 224b is interposed between the side wall 224b and the side wall 324b of the inner tray 300b at the lowest stage, so that the closest approach is shown in FIG.
  • the culture solution can be easily distributed to the lowermost culture space m1 of the culture flask 100b.
  • the fact that the culture flask of the present invention can avoid the flow of the culture solution even when the internal multistage tray is misaligned is not limited to the case of the other modes described with reference to FIGS. From the viewpoint of the effect of avoiding the distribution trouble, it goes without saying that the effect can be obtained more advantageously in the first embodiment including both the spacer 250 and the meat stealing portion 355.
  • FIG. 20 and 21 show other examples of the notch portion 351 and the meat stealing portion 355 provided on the side wall 324 of the internal tray 300.
  • FIG. 20 and 21 correspond to FIG. 6 of the first embodiment.
  • the notch portion 351c may be provided with a width wc wider than the width w of the meat stealing portion 355c.
  • the meat stealing portion 355d may be provided with a width wd wider than the width w of the notch portion 351d.
  • the spacer 250 is disposed in the vicinity of the port 280 side of the first communication portion 450 (see FIG. 1), but may be disposed in the vicinity of the opposite side of the port 280.
  • the spacer 250 is continuously provided on the holding unit 230 (see FIG. 2), but the holding unit 230 and the spacer 250 may be provided separately.
  • the vertical length of the spacer 250 is the height of one stage of the inner tray 300 in this embodiment (see FIG. 4), but may be any height.
  • the spacer 250 may be disposed so as to extend to the entire depth of the external casing 200 (the vertical length of the inner peripheral surface of the external rising peripheral wall 220).
  • FIG. 22 and 23 show another example of the external housing 200.
  • the inclined walls 225e and 226e may be connected to the end wall 221e directly from the side walls 223e and 224e, respectively.
  • the inclined walls 225f and 226f are connected to the end wall 221f directly from the side walls 223f and 224f, respectively, and the lower inclined wall 211f is connected to the outer bottom surface portion 210f.
  • leg portions 215f may protrude from the lower inclined wall 211f.
  • maintenance part 230 is provided in the side walls 223 and 224, it is not limited to this aspect.
  • the number and arrangement locations of the holding portions 230 are not limited as long as the holding portions 230 hold the inner multi-stage tray 400 so that the inner bottom portion 410 is parallel to the outer bottom portion 210 and has a predetermined height. Therefore, the same holding
  • the port 280 is provided such that its axis is located at the center between the side walls 223 and 224.
  • the port 280 is not limited to this aspect, and the axis of the port 280 is the side wall. 223 and 224 may be provided so as to deviate from the center.
  • the axis of the port 280 is provided so as to be parallel to the longitudinal direction of the external housing 200, but is not limited to this aspect, and is not parallel to the longitudinal direction. There may be.
  • the position and shape of the port 280 may be appropriately designed by those skilled in the art from the viewpoint of, for example, decantation (see FIG. 11 (V)).
  • the cell culture flasks 100, 100a, 100b have a simple configuration, but prevent liquid stored in the uppermost internal trays 300, 300a, 300b from undesirably leaking due to capillary action. be able to. For this reason, for example, the height h of the internal rising peripheral wall portion 320 (side walls 324, 324a, 324b, 324c, 324d) of the internal trays 300, 300a, 300b is reduced so that the external casings 200, 200a, 200b, 200e, 200f
  • the culture efficiency can be improved by increasing the number of stacked layers of the internal trays 300, 300a, and 300b for the depth.
  • the internal multi-stage trays 400, 400a, 400b are accommodated in the external housings 200, 200a, 200b, 200e, 200f, even if there is a stacking shift of the internal multi-stage trays 400, 400a, 400b, external dimensions ( The occurrence of individual product differences (corresponding to the dimensions of the external casings 200, 200a, 200b, 200e, and 200f) can be eliminated, and handling by the machine in the manufacturing process is facilitated.
  • the cell culture flasks 100 and 100a have meat stealing portions 355, 355a, 355c, and 355d that communicate with the first communication holes CH1, CH1a, and CH1b on the outer peripheral side of the inner rising peripheral wall portion 320 (side walls 324, 324a, 324c, and 324d).
  • the inner trays 300 and 300a are formed over the entire stacking direction, the liquid flow in the first communication holes CH1 and CH1a becomes smoother.
  • the meat stealing portions 355, 355a, 355c, and 355d are provided on the inner rising peripheral wall portion 320 (side walls 324, 324a, 324b, 324c, and 324d), and the inner bottom surface portion 310 does not have a hole for communication. There is no loss of culture volume. Therefore, the culture volume can be secured efficiently.
  • the cell culture flasks 100 and 100b are in contact with the vicinity of the first communication holes CH1 and CH1b of the internal rising peripheral wall 320 (side walls 324 and 324b) on the inner peripheral side of the external rising peripheral wall 220 (side walls 224 and 224b). Since the spacers 250 and 250b are protruded, the liquid flow in the first communication holes CH1 and CH1b becomes smoother.
  • the cell culture flasks 100, 100a, 100b are arranged so that the inner multi-stage trays 400, 400a, 400b are placed at a predetermined height from the outer bottom surface portions 210, 210a, 210b on the inner peripheral side of the outer rising peripheral wall portion 220 (side walls 224, 224a, 224b). Since the holding portions 230, 230a, and 230b that are separated and held are protruded, the external bottom surface portions 210, 210a, and 210b can also be used as the culture space m1.
  • the length between the outer rising peripheral wall portions 220 (end walls 221, 222) facing the longitudinal direction of the outer casing 200 is the outer rising peripheral wall portion 220 facing the shorter direction of the outer casing 200. Since it is 1.9 times or more and 2.2 times or less of the length between (side walls 223, 224), it is easy to carry with one hand even though it is a multistage flask. Since the cell culture flask 100 of the present invention can prevent undesired liquid leakage from the inner tray 300, such a long type shape that is easily affected by inclination is also allowed. In addition, because of the long type, for example, a sufficient culture volume can be secured even if the depth per stage is reduced relative to the capacity per stage of the internal tray 300. Therefore, the number of stacked layers can be increased for the depth of the cell, and thus the culture efficiency can be further improved.
  • the inner bottom surfaces of the inner trays 300, 300a, 300b have a thickness of 1.2 mm or more and 2.0 mm or less, so that the capacity of the inner trays 300, 300a, 300b can be reduced.
  • the height h of the internal rising peripheral wall 320 (side walls 324, 324a, 324b, 324c, 324d) can be reduced. For this reason, it is possible to increase the number of stacking stages for the depth of the outer casing 200, and thus it is possible to further improve the culture efficiency.
  • the thickness of the inner bottom surface of the inner multi-stage trays 400, 400a, 400b is relatively Even if it is small, the strength of the entire cell culture flask 100, 100a, 100b is guaranteed.
  • the height h of the internal rising peripheral wall 320 (side walls 324, 324a, 324b, 324c, 324d) of the internal trays 300, 300a, 300b is 8.5 mm or more and 11.0 mm or less. Therefore, the number of stacked layers can be increased for the depth of the outer casings 200, 200a, and 200b, and the culture efficiency can be further improved.
  • the cell culture flask 100 includes first inclined walls 225 and 226 and second inclined walls 227 and 228 in the outer rising peripheral wall portion 220, and the inner rising peripheral wall portion 320 in the internal multi-stage tray 400 is the outer rising peripheral wall portion 220.
  • the first inclined walls 227 and 228 are formed along the side walls 223 and 224, the end wall 222, and the first inclined walls 225 and 226.
  • the cell culture flasks 100, 100a, 100b correspond to “cell culture flasks”
  • the external bottom surface portions 210, 210a, 210b, 210f correspond to “external bottom surface portions”
  • the external rising peripheral wall portions 220 are “ Corresponding to “external rising peripheral wall portion”
  • port 280 corresponds to “port”
  • external housings 200, 200a, 200b, 200e, 200f correspond to “external housing”
  • internal bottom surface portion 310 corresponds to “internal bottom surface portion”.
  • the internal rising peripheral wall 320 corresponds to “internal rising peripheral wall”, the internal trays 300, 300a, 300b correspond to “internal tray”, and the upper end 329 corresponds to “upper end of the internal rising peripheral wall”.
  • the inner multi-stage trays 400, 400a, 400b correspond to “inner multi-stage trays”, and the upper end 229 is “the upper end of the external rising peripheral wall portion”.
  • the upper end 429 corresponds to “the upper end of the internal rising peripheral wall portion of the uppermost internal tray”, the lid 500 corresponds to the “lid”, and the first communication holes CH1, CH1a, CH1b are “communication holes”.
  • the meat stealing portions 355, 355a, 355c, and 355d correspond to “meat stealing portions”, the spacers 250 and 250b correspond to “spacers”, the height H corresponds to “predetermined height”,
  • the holding portions 230, 230a, and 230b correspond to the “holding portion”, the height h corresponds to the “height of the internal rising peripheral wall portion”, the side walls 223, 224, 224a, and 224b correspond to the “side wall”,
  • the walls 221, 222, 221e, 221f correspond to “end walls”, the first inclined walls 225, 226 correspond to “first inclined side walls”, and the second inclined walls 227, 228 become “second inclined side walls”.
  • Corresponding to the notches 351, 351a, 51b, 351c, 351d is equivalent to "the notch”.

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Abstract

Provided is a cell culture flask which has a simple structure and can prevent that the liquid contained in a topmost tray leaks unintentionally through the capillary effect and in which differences in the outer dimensions in individual products are eliminated from occurring; also provided is a manufacturing method thereof. This cell culture flask (100) comprises: an outer frame (200) which includes an outer bottom surface part, a rising outer circumferential wall provided around the entire perimeter of the outer bottom surface part, and a port (280) provided in a portion of the rising peripheral wall; an multi-stage inner tray (400) which is housed inside of the outer frame and which comprises multiple stacked inner trays which include an inner bottom surface part and a rising inner peripheral wall provided around the entire perimeter of the inner bottom surface part; a lid (500) which covers and fuses to both the top end of the rising outer peripheral wall and the top end of the rising inner peripheral wall of the topmost inner tray of the multi-stage inner tray; and a communication hole formed in the rising inner peripheral wall of each of the multi-stage inner trays so as to allow communication between the spaces in the inner trays and the space in the outer frame.

Description

細胞培養フラスコおよび細胞培養フラスコの製造方法Cell culture flask and method for producing cell culture flask
　本発明は、細胞培養フラスコに関する。より具体的には、本発明は、培養効率をより向上させるための多段式培養フラスコに関する。 The present invention relates to a cell culture flask. More specifically, the present invention relates to a multistage culture flask for further improving the culture efficiency.
　従来から、複数段のトレイが積層された構造を有する細胞培養フラスコが提供されている。
　たとえば、国際公開第９３／１０２１１号パンフレット（特許文献１）に記載の培養容器においては、培養室を規定する積層されたトレイ部材が、平坦な底壁および端壁を有する外部筐体に収容されており、積層されたトレイ部材は、その周囲が互いに封止されており、上階のトレイ部材の端は、筐体の天面の内面から離隔されることで解放されており、液体は、端壁に沿って設けられたトンネルを通じて各培養室に分配される。 Conventionally, a cell culture flask having a structure in which a plurality of trays are stacked is provided.
For example, in the culture container described in International Publication No. 93/102111 (Patent Document 1), the stacked tray members that define the culture chamber are accommodated in an external casing having a flat bottom wall and end walls. The stacked tray members are sealed around each other, and the end of the upper floor tray member is released by being separated from the inner surface of the top surface of the casing, and the liquid is It is distributed to each culture room through a tunnel provided along the end wall.
　日本国特開２０１１－５２８２２６号公報（特許文献２）に記載の多段細胞培養システムは、上板、側壁及びポートを含むカバー；底板、下側部分及びギャップ領域を備えた細胞等収容用中間トレイ、但しギャップ領域は、中間トレイ底板の内部部分で外側に曲げられたリップと、外側に曲げられた中間トレイ底板の隣接した側壁部分との間に形成され、リップは外側への曲線状エッジ態様を有する；並びに底板及び側壁を備える細胞等収容用ベーストレイ；を備える。上記中間トレイはカバー／ベーストレイ間に配置され、中間トレイ底板のギャップ領域はカバー上のポートと一直線上にあり、ポート、中間トレイ及びベーストレイを互いに流体連通させて、各中間トレイ及びベーストレイ上に配置された細胞等を除去及び／又は添加するため直接アクセス可能としている。上記中間トレイは、互いの上部に積み重ねられた複数であり、各中間トレイのギャップ領域は互いに一直線上にある。 A multistage cell culture system described in Japanese Patent Application Laid-Open No. 2011-528226 (Patent Document 2) includes a cover including an upper plate, a side wall, and a port; an intermediate tray for storing cells and the like having a bottom plate, a lower portion, and a gap region However, the gap region is formed between the lip bent outward at the inner portion of the intermediate tray bottom plate and the adjacent side wall portion of the intermediate tray bottom plate bent outward, and the lip has a curved edge shape toward the outer side. And a base tray for accommodating cells and the like having a bottom plate and side walls. The intermediate tray is disposed between the cover / base tray, and the gap region of the intermediate tray bottom plate is in line with the port on the cover, and the intermediate tray and the base tray are in fluid communication with each other. Direct access is possible to remove and / or add cells and the like placed above. The intermediate trays are plural stacked on top of each other, and the gap regions of the intermediate trays are in line with each other.
　日本国特開２０１１－２４５７７号公報（特許文献３）に記載の多層の組織培養容器は、基部を含み且つ基部の境界を少なくとも部分的になす上方に延びた壁を備える底部と、基部を含み且つ基部の境界を少なくとも部分的になす下方に延びた壁を備える頂部と、開口を画成する管状首部と、一以上の棚部とを含み、各棚部が基部と、基部の境界を少なくとも部分的になす上方に延びた壁とを備える。第１の棚部の上方に延びる壁が頂部の下方に延びる壁に隣接し、第１の棚部が底部と頂部の間に位置される。各棚部の基部がこれに形成された少なくとも一つの穴を有する。底部、頂部および一以上の棚部が、集合して、細胞を培養するための囲まれた容積を画成する。管状首部が容器から延び、囲まれた容積が、管状首部の開口によってアクセス可能である。 A multilayer tissue culture container described in Japanese Patent Application Laid-Open No. 2011-24577 (Patent Document 3) includes a base including a base and a bottom including an upwardly extending wall that at least partially forms a boundary of the base. And a top having a downwardly extending wall that at least partially defines a base boundary, a tubular neck defining an opening, and one or more shelves, each shelf at least defining a base and a base boundary A partially extending upwardly extending wall. A wall extending above the first shelf is adjacent to a wall extending below the top, and the first shelf is located between the bottom and the top. The base of each shelf has at least one hole formed in it. The bottom, top, and one or more shelves aggregate to define an enclosed volume for culturing cells. A tubular neck extends from the container and the enclosed volume is accessible by an opening in the tubular neck.
国際公開第９３／１０２１１号パンフレットInternational Publication No. 93/102111 Pamphlet 日本国特開２０１１－５２８２２６号公報Japanese Unexamined Patent Publication No. 2011-528226 日本国特開２０１１－２４５７７号公報Japanese Unexamined Patent Publication No. 2011-24577
　しかしながら、特許文献１に記載の容器においては、最上段のトレイ部材の上端が天面から隔離されており、固定されていない。したがって、最上段のトレイ部材に収容された培養液が、毛管現象により、当該上端と天面とのわずかな隙間を伝ってトレイ部材外へ漏れ出すことがある。 However, in the container described in Patent Document 1, the upper end of the uppermost tray member is isolated from the top surface and is not fixed. Therefore, the culture solution stored in the uppermost tray member may leak out of the tray member through a slight gap between the upper end and the top surface due to capillary action.
　また、特許文献２および３に記載の容器においては、複数の棚部それぞれと底部と頂部とが全て積層されている。したがって、製造時における積層ずれが実質的に免れず、外部寸法の製品個体差が常に発生する。 In the containers described in Patent Documents 2 and 3, each of the plurality of shelves, the bottom, and the top are all laminated. Therefore, the stacking deviation at the time of manufacture is substantially unavoidable, and individual product differences in external dimensions always occur.
　本発明の目的は、中間トレイの最上段内の培養液が外に漏れ出すおそれがなく、かつ、外部寸法の製品個体差を排除可能な細胞培養フラスコを提供することにある。 An object of the present invention is to provide a cell culture flask in which the culture medium in the uppermost stage of the intermediate tray does not leak out and can eliminate the individual differences in the external dimensions.
（１）
　一局面に従う細胞培養フラスコは、外部筐体と、内部多段トレイと、蓋体とを含む。外部筐体は、外部底面部と、外部底面部の全周に設けられた外部立ち上がり周壁部と、外部立ち上がり周壁部の一部に設けられたポートを含む。内部多段トレイは、内部底面部と、内部底面部の全周に設けられた内部立ち上がり周壁部とを含む内部トレイが複数段積層され、内部立ち上がり周壁部の上端と、当該上端に積重なる内部底面部の下面とが互いに溶着され、且つ、外部筐体内に収容される。蓋体は、外部立ち上がり周壁部の上端と、内部多段トレイにおける最上段内部トレイの内部立ち上がり周壁部の上端との両方を被覆し、且つ、当該両方の上端と溶着する。さらに、内部多段トレイの各段における内部立ち上がり周壁部には、内部トレイ内の空間と外部筐体内の空間とを連通させる連通孔が形成される。 (1)
A cell culture flask according to one aspect includes an outer casing, an inner multi-stage tray, and a lid. The external housing includes an external bottom surface portion, an external rising peripheral wall portion provided on the entire periphery of the external bottom surface portion, and a port provided in a part of the external rising peripheral wall portion. The internal multi-stage tray is formed by stacking a plurality of internal trays including an internal bottom surface portion and an internal rising peripheral wall portion provided on the entire circumference of the internal bottom surface portion, and stacking on the upper end of the internal rising peripheral wall portion and the upper end. The lower surface of the part is welded to each other and accommodated in the external housing. The lid covers both the upper end of the outer rising peripheral wall portion and the upper end of the inner rising peripheral wall portion of the uppermost internal tray in the internal multi-stage tray, and is welded to both upper ends. Furthermore, a communication hole that connects the space in the internal tray and the space in the external housing is formed in the internal rising peripheral wall portion in each stage of the internal multi-stage tray.
　これによって、簡素な構成でありながら、最上段のトレイに収容された液体が毛管現象で不所望に漏れ出すことを防止することができる。このため、たとえば内部トレイの深さを小さくし、外部筐体の深さの割に内部トレイの積層段数を多くして、培養効率を向上させることができる。さらに、内部多段トレイが外部筐体に収容されているため、たとえ内部多段トレイの積層ずれがあったとしても外部寸法（外部筐体の寸法に相当）の製品個体差の発生を排除することができ、製造工程でのマシンによるハンドリングが容易となる。 This makes it possible to prevent the liquid stored in the uppermost tray from leaking undesirably due to capillary action, although it has a simple configuration. For this reason, for example, the depth of the internal tray can be reduced, and the number of stacking stages of the internal tray can be increased for the depth of the external housing, thereby improving the culture efficiency. Furthermore, since the internal multi-stage tray is housed in the external housing, even if there is a stacking deviation of the internal multi-stage tray, it is possible to eliminate the occurrence of individual differences in the external dimensions (corresponding to the dimensions of the external housing). And handling by a machine in the manufacturing process becomes easy.
　（２）
　本発明の細胞培養フラスコにおいては、内部立ち上がり周壁部の外周側において、連通孔と連通する肉盗み部が、内部トレイの積層方向全体にわたって形成されていてよい。 (2)
In the cell culture flask of the present invention, on the outer peripheral side of the internal rising peripheral wall portion, the meat stealing portion communicating with the communication hole may be formed over the entire stacking direction of the internal tray.
　これによって、連通孔内の液流がよりスムーズとなる。したがって、仮に内部トレイの積層にずれが生じることで、内部立ち上がり周壁部が外部立ち上がり周壁部に接触する部分が生じたとしても、当該接触部分近傍における連通孔内の液流障害を回避することができる。また、内部底面部に連通のための孔を設けない構造が可能となるため、培養容積のロスが無く、効率よく培養容積を確保することができる。 This makes the liquid flow in the communication hole smoother. Therefore, even if there is a portion where the internal rising peripheral wall portion comes into contact with the external rising peripheral wall portion due to a deviation in the stacking of the internal trays, it is possible to avoid a liquid flow failure in the communication hole in the vicinity of the contact portion. it can. Moreover, since a structure in which a hole for communication is not provided in the inner bottom surface portion is possible, there is no loss of culture volume, and the culture volume can be efficiently secured.
（３）
　本発明の細胞培養フラスコにおいては、外部立ち上がり周壁部の内周側において、内部立ち上がり周壁部の連通孔近傍に接触するようにスペーサが突設されてよい。 (3)
In the cell culture flask of the present invention, a spacer may protrude from the inner peripheral side of the outer rising peripheral wall portion so as to contact the vicinity of the communication hole of the inner rising peripheral wall portion.
　これによって、連通孔内の液流がよりスムーズとなる。したがって、仮に内部トレイの積層にずれが生じることで、内部立ち上がり周壁部が外部立ち上がり周壁部に接触する部分が生じたとしても、当該接触部分近傍における連通孔内の液流障害を回避することができる。 This makes the liquid flow in the communication hole smoother. Therefore, even if there is a portion where the internal rising peripheral wall portion comes into contact with the external rising peripheral wall portion due to a deviation in the stacking of the internal trays, it is possible to avoid a liquid flow failure in the communication hole in the vicinity of the contact portion. it can.
（４）
　本発明の細胞培養フラスコにおいては、外部立ち上がり周壁部の内周側において、内部多段トレイを外部底面部から所定の高さで離間させて保持する保持部が突設されてよい。この場合、保持部は、スペーサに連設するように設けられる。 (4)
In the cell culture flask of the present invention, on the inner peripheral side of the outer rising peripheral wall portion, a holding portion that holds the inner multi-stage tray separated from the outer bottom surface portion at a predetermined height may be provided. In this case, the holding part is provided so as to be connected to the spacer.
　これによって、外部底面部も培養スペースとして利用することができる。さらにこの場合、仮に内部トレイの積層にずれが生じることで、最下段の内部トレイの内部立ち上がり周壁部が外部立ち上がり周壁部に接触する部分が生じたとしても、当該培養スペースへスムーズに培養液を分配することができる。 Thus, the outer bottom surface can also be used as a culture space. Furthermore, in this case, even if the inner tray stacking is displaced, and the inner rising peripheral wall portion of the lowermost internal tray has a portion in contact with the outer rising peripheral wall portion, the culture medium can be smoothly transferred to the culture space. Can be distributed.
（５）
　本発明の細胞培養フラスコにおいては、外部筐体の長手方向に対向する外部立ち上がり周壁部間の長さが、外部筐体の短手方向に対向する外部立ち上がり周壁部間の長さの１．９倍以上２．２倍以下であってよい。 (5)
In the cell culture flask of the present invention, the length between the external rising peripheral wall portions facing in the longitudinal direction of the external housing is 1.9 of the length between the external rising peripheral wall portions facing in the short direction of the external housing. It may be not less than twice and not more than 2.2 times.
　このため、多段フラスコでありながら、片手での持ち運びがしやすい。本発明の細胞培養フラスコは、内部トレイからの不所望の液体漏れを防止できるため、傾きの影響を受けやすいこのような長タイプの形状も許容される。また、長タイプであることによって、たとえば、内部トレイ１段当たりの容量の割に当該１段当たりの深さを小さくしても培養容積を充分確保することができるため、外部筐体の深さの割に積層段数をより多くすることができ、したがって、培養効率をより向上させることができる。 For this reason, it is easy to carry with one hand even though it is a multi-stage flask. Since the cell culture flask of the present invention can prevent undesired liquid leakage from the inner tray, such a long type shape that is easily affected by inclination is also allowed. In addition, because of the long type, for example, a sufficient culture volume can be ensured even if the depth per step is reduced relative to the capacity per step of the internal tray. However, it is possible to increase the number of stacking stages, and thus the culture efficiency can be further improved.
（６）
　本発明の細胞培養フラスコにおいては、内部底面部の厚みは、１．２ｍｍ以上２．０ｍｍ以下であってよい。 (6)
In the cell culture flask of the present invention, the thickness of the inner bottom surface portion may be 1.2 mm or more and 2.0 mm or less.
　これによって、内部トレイ１段当たりの容量の割に、内部立ち上がり周壁部の高さを小さくすることができるため、外部筐体の深さの割に積層段数をより多くすることができ、培養効率をより向上させることができる。
　本発明の細胞培養フラスコは、内部多段トレイが外部筐体に収容されているため、内部多段トレイの内部底面部の厚みが相対的に小さくても、細胞培養フラスコ全体としての強度が保証される。 As a result, the height of the internal rising peripheral wall portion can be reduced relative to the capacity per one stage of the internal tray, so that the number of stacked stages can be increased relative to the depth of the external casing, and the culture efficiency can be increased. Can be further improved.
In the cell culture flask of the present invention, since the internal multistage tray is housed in the outer casing, the strength of the entire cell culture flask is guaranteed even if the thickness of the inner bottom surface of the internal multistage tray is relatively small. .
（７）
　本発明の細胞培養フラスコにおいては、内部立ち上がり周壁部の高さが、８．５ｍｍ以上１１．０ｍｍ以下であってよい。 (7)
In the cell culture flask of the present invention, the height of the internal rising peripheral wall portion may be 8.5 mm or more and 11.0 mm or less.
　これによって、外部筐体の深さの割に積層段数をより多くすることができ、培養効率をより向上させることができる。 This makes it possible to increase the number of stacked layers for the depth of the outer casing, and to further improve the culture efficiency.
（８）
　本発明の細胞培養フラスコにおいては、外部立ち上がり周壁部が、対向する一対の側壁と、対向する一対の端壁と、傾斜側壁とを含んでよい。この場合、対向する一対の端壁のうち一方にはポートが形成されており、傾斜側壁は一対の側壁の少なくとも一方から延び且つ上方からの平面視において、ポートの根元に向かって傾斜している。さらにこの場合、傾斜側壁は第１傾斜側壁と第２傾斜側壁とを含む。第１傾斜側壁は側壁から延び、ポートの軸心に対してより小さい角度で傾斜する。第２傾斜側壁は第１傾斜側壁から延び、ポートの軸心に対してより大きい角度で傾斜する。なおかつこの場合、内部多段トレイにおける内部立ち上がり周壁部は、外部立ち上がり周壁部のうち、側壁、端壁の他方（すなわちポートが形成されていないほう）、および第１傾斜側壁に沿うように形成される。 (8)
In the cell culture flask of the present invention, the external rising peripheral wall portion may include a pair of opposing side walls, a pair of opposing end walls, and an inclined side wall. In this case, a port is formed in one of the pair of opposing end walls, and the inclined side wall extends from at least one of the pair of side walls and is inclined toward the root of the port in plan view from above. . Further, in this case, the inclined side wall includes a first inclined side wall and a second inclined side wall. The first inclined sidewall extends from the sidewall and is inclined at a smaller angle with respect to the port axis. The second inclined sidewall extends from the first inclined sidewall and is inclined at a larger angle with respect to the port axis. In this case, the internal rising peripheral wall portion of the internal multistage tray is formed along the side wall, the other of the end walls (that is, the side where the port is not formed) and the first inclined side wall among the external rising peripheral wall portions. .
　したがって、第２傾斜側壁によってポートからのデカンテーションを容易にしつつも、より小さい角度で傾斜する第１傾斜側壁によって、１段あたりの培養容積をより大きくとることができる。 Therefore, while the decantation from the port is facilitated by the second inclined side wall, the culture volume per stage can be increased by the first inclined side wall inclined at a smaller angle.
（９）
　他の局面に従う細胞培養フラスコの製造方法は、積層工程と、覆蓋工程と、収容工程とを含む。
　積層工程においては、内部底面部と、内部底面部の全周に設けられた内部立ち上がり周壁部と、内部立ち上がり周壁部の上端の一部が切欠かれた切欠き部とを含む内部トレイを、複数積層し、内部立ち上がり周壁部の上端と、当該上端に積重なる内部底面部の下面とを互いに溶着させ、内部多段トレイを得る。
　覆蓋工程においては、内部多段トレイにおける最上段内部トレイの内部立ち上がり周壁部の上端を蓋体で被覆し、上端と蓋体とを溶着し、覆蓋された内部多段トレイを得る。
　収容工程においては、外部底面部の全周に設けられた外部立ち上がり周壁部と、外部立ち上がり周壁部の一部に設けられたポートとを含む外部筐体の内部に、覆蓋された内部多段トレイを収容し、外部立ち上がり周壁部の上端と蓋体とを溶着し、内部多段トレイ収容筐体を得る。 (9)
The method for producing a cell culture flask according to another aspect includes a stacking step, a covering step, and a containing step.
In the laminating step, a plurality of internal trays including an inner bottom surface portion, an inner rising peripheral wall portion provided on the entire circumference of the inner bottom surface portion, and a notch portion in which a part of the upper end of the inner rising peripheral wall portion is notched. The upper end of the inner rising peripheral wall portion and the lower surface of the inner bottom surface portion stacked on the upper end are welded together to obtain an internal multi-stage tray.
In the covering step, the upper end of the inner rising peripheral wall portion of the uppermost inner tray in the inner multi-stage tray is covered with a lid, and the upper end and the lid are welded to obtain the covered inner multi-stage tray.
In the housing step, an internal multi-stage tray with a cover is placed inside an external housing including an external rising peripheral wall portion provided on the entire circumference of the external bottom surface portion and a port provided in a part of the external rising peripheral wall portion. Accommodating and welding the upper end of the external rising peripheral wall and the lid to obtain an internal multi-stage tray housing case.
　これによって、簡素な構成でありながら、最上段のトレイに収容された液体が毛管現象で不所望に漏れ出すことを防止可能であり、かつ外部寸法の製品個体差の発生を排除した細胞培養フラスコを製造することができる。 This makes it possible to prevent the liquid contained in the uppermost tray from leaking undesirably due to capillary action, while eliminating the occurrence of individual product differences in external dimensions. Can be manufactured.
第１実施形態にかかる培養フラスコ１００の一例を示す模式的外観斜視図である。It is a typical external appearance perspective view which shows an example of the culture flask 100 concerning 1st Embodiment. 図１の培養フラスコ１００の模式的分解図である。It is a typical exploded view of the culture flask 100 of FIG. 図１のＡ－Ａ線断面図である。FIG. 2 is a sectional view taken along line AA in FIG. 1. 図１のＢ－Ｂ線断面図である。FIG. 2 is a sectional view taken along line BB in FIG. 図１の培養フラスコ１００の一部を示す模式的外観斜視図である。It is a typical external appearance perspective view which shows a part of culture flask 100 of FIG. 図５の丸囲み部分の拡大図である。FIG. 6 is an enlarged view of a circled portion in FIG. 5. 図１のＣ－Ｃ線断面図である。FIG. 2 is a cross-sectional view taken along the line CC of FIG. 図１の一部の切欠き拡大図である。FIG. 2 is a partially cutaway enlarged view of FIG. 1. 培養フラスコ１００の製造方法の工程を示す模式図である。FIG. 4 is a schematic diagram showing a process of a method for manufacturing a culture flask 100. 培養フラスコ１００の製造方法の工程を示す模式図である。FIG. 4 is a schematic diagram showing a process of a method for manufacturing a culture flask 100. 培養フラスコ１００の操作方法の工程を示す説明図である。It is explanatory drawing which shows the process of the operating method of the culture flask. 培養フラスコ１００の操作方法の工程を示す説明図である。It is explanatory drawing which shows the process of the operating method of the culture flask. 培養フラスコ１００の操作方法の工程を示す説明図である。It is explanatory drawing which shows the process of the operating method of the culture flask. 培養フラスコ１００の他の例を示す一部切欠き拡大図である。It is a partially cutaway enlarged view showing another example of the culture flask 100. 図１４の断面図である。It is sectional drawing of FIG. 図１４の断面図である。It is sectional drawing of FIG. 培養フラスコ１００の他の例を示す一部切欠き拡大図である。It is a partially cutaway enlarged view showing another example of the culture flask 100. 図１７の断面図である。It is sectional drawing of FIG. 図１７の断面図である。It is sectional drawing of FIG. 培養フラスコ１００の他の例を示す一部拡大図である。4 is a partially enlarged view showing another example of the culture flask 100. FIG. 培養フラスコ１００の他の例を示す一部拡大図である。4 is a partially enlarged view showing another example of the culture flask 100. FIG. 培養フラスコ１００の他の例を示す一部拡大図である。4 is a partially enlarged view showing another example of the culture flask 100. FIG. 培養フラスコ１００の他の例を示す一部拡大図である。4 is a partially enlarged view showing another example of the culture flask 100. FIG.
１００，１００ａ，１００ｂ　細胞培養フラスコ
２００，２００ａ，２００ｂ，２００ｅ，２００ｆ　外部筐体
２１０，２１０ａ，２１０ｂ，２１０ｆ　外部底面部
２２０　外部立ち上がり周壁部
２２１，２２２，２２１ｅ，２２１ｆ　端壁
２２３，２２４，２２４ａ，２２４ｂ　側壁
２２５，２２６　第１傾斜壁
２２７，２２８　第２傾斜壁
２２９　（外部立ち上がり周壁部の）上端
２３０，２３０ａ，２３０ｂ　保持部
２５０，２５０ｂ　スペーサ
２８０　ポート
３００，３００ａ，３００ｂ　内部トレイ
３１０　内部底面部
３２０　内部立ち上がり周壁部
３２９　（内部立ち上がり周壁部の）上端
３５１，３５１ａ，３５１ｂ，３５１ｃ，３５１ｄ　切欠き部
３５５，３５５ａ，３５５ｃ，３５５ｄ　肉盗み部
４００，４００ａ，４００ｂ　内部多段トレイ
４２９　（最上段内部トレイの内部立ち上がり周壁部の）上端
５００　蓋体
ＣＨ１，ＣＨ１ａ，ＣＨ１ｂ　第１連通孔
ｈ　（内部立ち上がり周壁部の）高さ
Ｈ　（外部底面部上面から内部多段トレイの内部底面部下面までの）高さ 100, 100a, 100b Cell culture flask 200, 200a, 200b, 200e, 200f External casing 210, 210a, 210b, 210f External bottom surface part 220 External rising peripheral wall parts 221, 222, 221e, 221f End walls 223, 224, 224a, 224b Side walls 225, 226 First inclined wall 227, 228 Second inclined wall 229 Upper end 230 (230a, 230b) of holding wall 250, 250b Spacer 280 Port 300, 300a, 300b Internal tray 310 Internal bottom surface 320 Internal rising peripheral wall 329 (Upper internal rising peripheral wall) upper ends 351, 351a, 351b, 351c, 351d Notch portions 355, 355a, 355c, 355d Meat stealing portions 400, 400a, 400b Internal multistage tray 429 Upper end 500 (of the inner rising peripheral wall portion of the uppermost internal tray) Lid CH1, CH1a, CH1b First communication hole h (of the inner rising peripheral wall portion) Height H (From the upper surface of the outer bottom surface portion to the lower surface of the inner bottom surface of the inner multi-stage tray Up to)
　以下、図面を参照しつつ、本発明の実施の形態について説明する。以下の説明では、同一の要素には同一の符号を付しており、それらの名称および機能も同じである。したがって、それらについての詳細な説明は繰り返さない。 Hereinafter, embodiments of the present invention will be described with reference to the drawings. In the following description, the same elements are denoted by the same reference numerals, and their names and functions are also the same. Therefore, detailed description thereof will not be repeated.
［第１実施形態］
　図１は、第１実施形態にかかる培養フラスコ１００の一例を示す模式的外観斜視図である。説明の便宜上、図１の上側を上、下側を下と呼称する。図２は、図１の培養フラスコ１００の模式的分解図である。図３は、図１のＡ－Ａ線断面図である。図４は、図１のＢ－Ｂ線断面図である。図５は、図１の培養フラスコ１００の一部を示す模式的外観斜視図である。図６は、図５の丸囲み部分の拡大図である。図７は、図１のＣ－Ｃ線断面図である。図８は、図１の一部の切欠き拡大図である。 [First Embodiment]
FIG. 1 is a schematic external perspective view showing an example of a culture flask 100 according to the first embodiment. For convenience of explanation, the upper side of FIG. FIG. 2 is a schematic exploded view of the culture flask 100 of FIG. 3 is a cross-sectional view taken along line AA in FIG. 4 is a cross-sectional view taken along line BB of FIG. FIG. 5 is a schematic external perspective view showing a part of the culture flask 100 of FIG. FIG. 6 is an enlarged view of a circled portion in FIG. 7 is a cross-sectional view taken along the line CC of FIG. FIG. 8 is an enlarged view of a part of FIG.
［基本構成］
　図１および図２に示すように、培養フラスコ１００は、外部筐体２００と、内部多段トレイ４００と、蓋体５００とを含む。外部筐体２００は、内部多段トレイ４００を収容する。蓋体５００は、外部筐体２００と内部多段トレイ４００とを覆蓋する。また、培養フラスコ１００は、内部多段トレイ４００に設けられた第１連通部４５０と第２連通部４８０とを含む。さらに、培養フラスコ１００は、外部筐体２００に装着されたキャップ２９０を含む。 [Basic configuration]
As shown in FIGS. 1 and 2, the culture flask 100 includes an external housing 200, an internal multistage tray 400, and a lid 500. The external housing 200 accommodates the internal multistage tray 400. The lid 500 covers the outer casing 200 and the inner multi-stage tray 400. The culture flask 100 includes a first communication portion 450 and a second communication portion 480 provided on the internal multi-stage tray 400. Furthermore, the culture flask 100 includes a cap 290 attached to the outer casing 200.
［外部筐体］
　図２に示すように、外部筐体２００は、外部底面部２１０と、その全周に設けられた外部立ち上がり周壁部２２０とを含む。外部立ち上がり周壁部２２０は、外部筐体２００の長手方向に対向する一対の端壁２２１，２２２と、短手方向に対向する一対の側壁２２３，２２４とを含む。一方の端壁２２１には、ポート２８０が設けられている。図３に示すように、ポート２８０は、端壁２２１から外部筐体２００の外部へ向かって突出し、且つ、外部筐体２００の内部と外部とを連通させる管状に形成されている。ポート２８０の軸心は、外部筐体２００の長手方向に平行であり、側壁２２３，２２４間の中央に位置する。ポート２８０には、図１および図２に示すようにキャップ２９０が着脱自在に装着される。 [External housing]
As shown in FIG. 2, the external housing 200 includes an external bottom surface portion 210 and an external rising peripheral wall portion 220 provided on the entire periphery thereof. The external rising peripheral wall portion 220 includes a pair of end walls 221 and 222 that face the longitudinal direction of the external housing 200 and a pair of side walls 223 and 224 that face the short side direction. One end wall 221 is provided with a port 280. As shown in FIG. 3, the port 280 protrudes from the end wall 221 toward the outside of the external housing 200, and is formed in a tubular shape that allows the inside and the outside of the external housing 200 to communicate with each other. The axis of the port 280 is parallel to the longitudinal direction of the outer casing 200 and is located at the center between the side walls 223 and 224. A cap 290 is detachably attached to the port 280 as shown in FIGS.
　図２に示すように、外部立ち上がり周壁部２２０は、さらに、ポート２８０の根元に向かって傾斜する傾斜壁を有する。傾斜壁は、上部から下部への平面視において、ポート２８０の軸心に対して線対称となるように構成される。傾斜壁は、一対の第１傾斜壁２２５，２２６および一対の第２傾斜壁２２７，２２８を含む。第１傾斜壁２２５，２２６は、それぞれ、側壁２２３，２２４に連設される。第２傾斜壁２２７，２２８は、それぞれ、第１傾斜壁２２５，２２６に連設され、共に、端壁２２１に連設される。第２傾斜壁２２７，２２８は、上部から下部への平面視において、ポート２８０の軸心に向かって、第１傾斜壁２２５，２２６より大きい角度で傾斜する。 As shown in FIG. 2, the external rising peripheral wall portion 220 further has an inclined wall inclined toward the root of the port 280. The inclined wall is configured to be line-symmetric with respect to the axis of the port 280 in a plan view from the upper part to the lower part. The inclined walls include a pair of first inclined walls 225 and 226 and a pair of second inclined walls 227 and 228. The first inclined walls 225 and 226 are connected to the side walls 223 and 224, respectively. The second inclined walls 227 and 228 are connected to the first inclined walls 225 and 226, respectively, and are both connected to the end wall 221. The second inclined walls 227 and 228 are inclined at an angle larger than the first inclined walls 225 and 226 toward the axis of the port 280 in a plan view from the upper part to the lower part.
　図２に示すように、外部立ち上がり周壁部２２０の内周面には、後述する保持部２３０が複数突設されている。保持部２３０は、外部筐体２００内で、内部多段トレイ４００を所定の高さ位置に保持するように設けられる。具体的には図４に示すように、保持部２３０の上端をなす保持面２３１が、内部多段トレイ４００の最下面（後述の内部底面部４１０の下面）と接触し、且つ、保持面２３１の位置が、外部底面部２１０の上面から高さＨだけ離間した位置となる。 As shown in FIG. 2, a plurality of holding portions 230 to be described later project from the inner peripheral surface of the external rising peripheral wall portion 220. The holding unit 230 is provided so as to hold the internal multi-stage tray 400 at a predetermined height position in the outer casing 200. Specifically, as shown in FIG. 4, the holding surface 231 that forms the upper end of the holding unit 230 is in contact with the lowermost surface of the internal multi-stage tray 400 (the lower surface of the inner bottom surface portion 410 described later), and the holding surface 231 The position is a position separated from the upper surface of the external bottom surface part 210 by a height H.
　このように、保持部２３０によって、外部筐体２００内には、外部底面部２１０、内部多段トレイ４００の最下面（後述の内部底面部４１０の下面）、および外部立ち上がり周壁部２２０で囲まれる空間ｍ１が確保される。保持部２３０によって確保されたこの空間ｍ１は、培養フラスコ１００において、内部多段トレイ４００内部の空間（培養空間ｍ２，～，ｍ６）と同様に培養空間として使用されることができる。以下において、空間ｍ１は培養空間ｍ１と記載する。 As described above, the space enclosed by the holding portion 230 in the outer casing 200 is the outer bottom surface portion 210, the lowermost surface of the internal multi-stage tray 400 (the lower surface of the inner bottom surface portion 410 described later), and the external rising peripheral wall portion 220. m1 is secured. This space m1 secured by the holding unit 230 can be used as a culture space in the culture flask 100 in the same manner as the space inside the internal multi-stage tray 400 (culture spaces m2,..., M6). Hereinafter, the space m1 is referred to as a culture space m1.
　図２に示すように、外部立ち上がり周壁部２２０の内周面のうち、第１連通部４５０の近傍に、スペーサ２５０が突設される。スペーサ２５０は、外部筐体２００内の第１連通部４５０で、内部多段トレイ４００の外周面と外部筐体２００の内周面との間に空隙を確保するように設けられる。具体的には図４に示すように、スペーサ２５０は、側壁２２４の内周面から高さＩで突設され、内部多段トレイ４００の外周面（後述の側壁３２４）と接触する。これによって、当該外周面（側壁３２４）と、外部筐体２００の側壁２２４の内周面との間にスペーサ２５０の高さＩに相当する幅の空隙を確保する。 As shown in FIG. 2, a spacer 250 protrudes in the vicinity of the first communication portion 450 on the inner peripheral surface of the external rising peripheral wall portion 220. The spacer 250 is provided in the first communication portion 450 in the outer casing 200 so as to secure a gap between the outer peripheral surface of the internal multi-stage tray 400 and the inner peripheral surface of the outer casing 200. Specifically, as shown in FIG. 4, the spacer 250 protrudes from the inner peripheral surface of the side wall 224 at a height I, and comes into contact with the outer peripheral surface (side wall 324 described later) of the internal multi-stage tray 400. As a result, a gap having a width corresponding to the height I of the spacer 250 is secured between the outer peripheral surface (side wall 324) and the inner peripheral surface of the side wall 224 of the external housing 200.
　長手方向に対向する一対の端壁２２１，２２２間の長さは、２１０ｍｍ以上２４０ｍｍ以下、例えば２２３ｍｍである。長手方向に対向する一対の端壁２２１，２２２間の長さは、短手方向に対向する一対の側壁２２３，２２４の長さの１．９倍以上２．２倍以下、例えば２．０倍である。第１傾斜壁２２５，２２６とポート２８０の軸心とが成す角は、４５度以上７０度以下、例えば６０度である。第２傾斜壁２２７，２２８と端壁２２１とが成す角は、３度以上１０度以下、例えば５度である。 The length between the pair of end walls 221 and 222 facing in the longitudinal direction is 210 mm or more and 240 mm or less, for example, 223 mm. The length between the pair of end walls 221 and 222 facing in the longitudinal direction is 1.9 to 2.2 times, for example, 2.0 times the length of the pair of side walls 223 and 224 facing in the short direction. It is. The angle formed by the first inclined walls 225 and 226 and the axis of the port 280 is not less than 45 degrees and not more than 70 degrees, for example, 60 degrees. The angle formed by the second inclined walls 227 and 228 and the end wall 221 is 3 degrees or more and 10 degrees or less, for example, 5 degrees.
［内部多段トレイ］
　内部多段トレイ４００は、図５に示す内部トレイ３００と同形同大のものが、複数積層されて構成される。本実施形態においては、５枚積層される。
　図５に示すように、内部トレイ３００は、内部底面部３１０と、その全周に設けられた内部立ち上がり周壁部３２０とを含む。内部トレイ３００の積層の態様としては、図２および図４に示すように、内部立ち上がり周壁部３２０の上端３２９と、上端３２９に積層される別の内部トレイ３００の内部底面部３１０の下面とが互いに溶着されるように積層される。これによって、内部多段トレイ４００は、内部トレイ３００同士で囲まれた培養空間ｍ２，～，ｍ５を有する。 [Internal multi-stage tray]
The internal multi-stage tray 400 is configured by stacking a plurality of the same and the same size as the internal tray 300 shown in FIG. In the present embodiment, five sheets are stacked.
As shown in FIG. 5, the internal tray 300 includes an internal bottom surface portion 310 and an internal rising peripheral wall portion 320 provided on the entire periphery thereof. As shown in FIGS. 2 and 4, the inner tray 300 is stacked in such a manner that the upper end 329 of the inner rising peripheral wall portion 320 and the lower surface of the inner bottom surface portion 310 of another inner tray 300 stacked on the upper end 329 are as follows. They are stacked so that they are welded together. Thus, the inner multi-stage tray 400 has culture spaces m2,..., M5 surrounded by the inner trays 300.
　なお、図２、図３および図４に記載のように、内部多段トレイ４００のうち最上段の内部トレイ３００の上端３２９を、特に、内部多段トレイ４００の上端４２９と表記する。また、内部多段トレイ４００のうち最下段の内部トレイ３００の内部底面部３１０を、特に、内部多段トレイ４００の内部底面部４１０と表記する。 Note that, as shown in FIGS. 2, 3, and 4, the upper end 329 of the uppermost internal tray 300 in the internal multi-stage tray 400 is particularly referred to as the upper end 429 of the internal multi-stage tray 400. In addition, the inner bottom surface portion 310 of the lowermost inner tray 300 among the inner multi-stage tray 400 is particularly referred to as an inner bottom surface portion 410 of the inner multi-stage tray 400.
　図５に示すように、内部立ち上がり周壁部３２０は、長手方向に対向する一対の端壁３２１，３２２と、短手方向に対向する一対の側壁３２３，３２４と、一対の傾斜壁３２５，３２６とを含む。一対の傾斜壁３２５，３２６は、側壁３２３，３２４のそれぞれおよび端壁３２１の両方に連設される。 As shown in FIG. 5, the internal rising peripheral wall portion 320 includes a pair of end walls 321 and 322 facing in the longitudinal direction, a pair of side walls 323 and 324 facing in the short direction, and a pair of inclined walls 325 and 326. including. The pair of inclined walls 325 and 326 are connected to both the side walls 323 and 324 and the end wall 321.
　図５に示すように、側壁３２４には、端壁３２１より端壁３２２に近い側、具体的には端壁３２２から距離ｌだけ隔てた位置に切欠き部３５１が設けられる。より具体的には、図６に示すように、側壁３２４に、上端３２９から下に凹となる切欠き部３５１が幅ｗで設けられる。側壁３２４には、さらに、外周面から内に凹となる肉盗み部３５５が、切欠き部３５１と同じ幅ｗで、側壁３２４の高さｈ方向全体に亘って設けられる。
　一方、図５に示すように、端壁３２１には、上端３２９から下に凹となる切欠き部３８１が設けられる。切欠き部３８１の深さは、所望の培養容積を確保できる深さであれば特に限定されるものではない。したがって、切欠き部３５１と同じ深さであってもよいし、異なる深さであってもよい。 As shown in FIG. 5, the side wall 324 is provided with a notch 351 at a position closer to the end wall 322 than the end wall 321, specifically, at a position separated from the end wall 322 by a distance l. More specifically, as shown in FIG. 6, the side wall 324 is provided with a notch 351 that is recessed downward from the upper end 329 with a width w. The side wall 324 is further provided with a meat stealing portion 355 that is recessed inwardly from the outer peripheral surface over the entire height h direction of the side wall 324 with the same width w as the notch portion 351.
On the other hand, as shown in FIG. 5, the end wall 321 is provided with a notch 381 that is recessed downward from the upper end 329. The depth of the notch 381 is not particularly limited as long as a desired culture volume can be ensured. Therefore, the same depth as the notch 351 may be sufficient, and a different depth may be sufficient.
　内部多段トレイ４００を構成する内部トレイ３００各段の端壁３２１，３２２は、内部多段トレイ４００が外部筐体２００内に収容された状態（図１参照）において、外部筐体２００の端壁２２１，２２２の面に沿うように配置される。同様に、内部多段トレイ４００における側壁３２３，３２４は、外部筐体２００の側壁２２３，２２４の面に沿い、内部多段トレイ４００における傾斜壁３２５，３２６は、外部筐体２００の第１傾斜壁２２５，２２６の面に沿うように配置される。 The end walls 321 and 322 of each stage of the internal tray 300 constituting the internal multistage tray 400 are the end walls 221 of the external casing 200 when the internal multistage tray 400 is accommodated in the external casing 200 (see FIG. 1). , 222 along the surface. Similarly, the side walls 323 and 324 of the inner multi-stage tray 400 are along the surfaces of the side walls 223 and 224 of the outer casing 200, and the inclined walls 325 and 326 of the inner multi-stage tray 400 are the first inclined walls 225 of the outer casing 200. , 226 along the surface.
　内部トレイ３００において、一対の端壁３２１，３２２間の長さは、２００ｍｍ以上２３０ｍｍ以下、たとえば２１４ｍｍである。また、一対の側壁３２３，３２４間の長さは、１００ｍｍ以上１１５ｍｍ以下、たとえば１０７ｍｍである。側壁３２４の高さｈは８．５ｍｍ以上１１．０ｍｍ以下、例えば９．０ｍｍである。内部トレイ３００の内部底面部３１０の厚さは、１．２ｍｍ以上２．０ｍｍ以下、例えば１．６ｍｍである。内部トレイ３００の深さは、７．０ｍｍ以上９．５ｍｍ以下、たとえば７．４ｍｍであってよい。内部トレイ３００の内部立ち上がり周壁部３２０の厚さは、内部底面部３１０の厚さと同様であってよい。切欠き部３５１の端壁３２２からの距離ｌは、外部筐体２００の長手方向に対向する一対の端壁２２１，２２２間の長さの１５％以上２５％以下、たとえば１９％である。切欠き部３５１の幅ｗは、外部筐体２００の端壁２２１，２２２間の長さの４％以上１０％以下、たとえば６％である。切欠き部３５１の深さｄは、高さｈの１０％以上３０％以下、たとえば１７％である。切欠き部３８１の幅は、外部筐体２００の短手方向に対向する一対の側壁２２３，２２４間の長さの４０％以上８０％以下、たとえば６０％である。 In the internal tray 300, the length between the pair of end walls 321 and 322 is 200 mm or more and 230 mm or less, for example, 214 mm. The length between the pair of side walls 323 and 324 is 100 mm or more and 115 mm or less, for example, 107 mm. The height h of the side wall 324 is 8.5 mm or more and 11.0 mm or less, for example, 9.0 mm. The thickness of the inner bottom surface portion 310 of the inner tray 300 is 1.2 mm or more and 2.0 mm or less, for example, 1.6 mm. The depth of the inner tray 300 may be 7.0 mm or more and 9.5 mm or less, for example, 7.4 mm. The thickness of the internal rising peripheral wall 320 of the internal tray 300 may be the same as the thickness of the internal bottom surface 310. The distance l from the end wall 322 of the notch 351 is 15% or more and 25% or less, for example 19%, of the length between the pair of end walls 221 and 222 facing the longitudinal direction of the external housing 200. The width w of the notch 351 is 4% or more and 10% or less, for example, 6% of the length between the end walls 221 and 222 of the external housing 200. The depth d of the notch 351 is 10% to 30% of the height h, for example, 17%. The width of the notch 381 is not less than 40% and not more than 80%, for example 60%, of the length between the pair of side walls 223 and 224 facing the short side direction of the outer casing 200.
［蓋体］
　蓋体５００は、図１に示すように、外部筐体２００の上部開口全体を覆蓋する形状で構成される。
　図３および図４に示すように、蓋体５００は、外部筐体２００を覆蓋すると共に、外部筐体２００に収容された内部多段トレイ４００も覆蓋する。この場合、蓋体５００の下面は、外部筐体２００の外部立ち上がり周壁部２２０の上端２２９と、内部多段トレイ４００の上端４２９の両方と溶着する。これによって、培養フラスコ１００は、内部多段トレイ４００の最上段の内部トレイ３００と蓋体５００とに囲まれた培養空間ｍ６を有する。したがって、培養フラスコ１００は、前述の培養空間ｍ１および培養空間ｍ２，～，ｍ５と合わせ、全部で６段の培養空間を含む。 [Lid]
As shown in FIG. 1, the lid 500 is configured to cover the entire upper opening of the external housing 200.
As shown in FIGS. 3 and 4, the lid 500 covers the outer casing 200 and also covers the internal multistage tray 400 accommodated in the outer casing 200. In this case, the lower surface of the lid 500 is welded to both the upper end 229 of the external rising peripheral wall 220 of the external housing 200 and the upper end 429 of the internal multi-stage tray 400. Thus, the culture flask 100 has a culture space m6 surrounded by the uppermost internal tray 300 and the lid 500 of the internal multi-stage tray 400. Therefore, the culture flask 100 includes the culture space m1 and the culture spaces m2,...
［第１連通部］
　前述のように、内部トレイ３００において、切欠き部３５１が端壁３２２から所定の距離ｌだけ隔てた位置に設けられ、切欠き部３８１が端壁３２１に設けられる（図５参照）ことにより、図１に示すように、第１連通部４５０は、第２連通部４８０と十分に隔離して配置される。第１連通部４５０は、通気のため、および培養液を各段に分配するために利用することができる。したがって、第１連通部４５０は、内部多段トレイ４００を構成する全ての内部トレイ３００の内部空間と、外部筐体２００の内部空間とを連通させるように構成される。 [First communication part]
As described above, in the internal tray 300, the notch 351 is provided at a position separated from the end wall 322 by a predetermined distance l, and the notch 381 is provided on the end wall 321 (see FIG. 5). As shown in FIG. 1, the first communication part 450 is disposed sufficiently separated from the second communication part 480. The first communication part 450 can be used for aeration and for distributing the culture solution to each stage. Accordingly, the first communication portion 450 is configured to communicate the internal spaces of all the internal trays 300 that constitute the internal multistage tray 400 with the internal spaces of the external housing 200.
　図４で述べたように、内部多段トレイ４００と外部筐体２００との間には、スペーサ２５０によって所定の空隙（図中、スペーサ２５０の高さＩに相当）が確保される。これに加えて、図６で述べたように、内部多段トレイの外周面（側壁３２４）には肉盗み部３５５が設けられている。肉盗み部３５５は、図７に示すように、内部トレイ３００の積層方向全体にわたって設けられるため、第１連通部４５０においては、内部多段トレイ４００と外部筐体２００との間の空隙Ｓが、肉盗み部３５５の分だけさらに幅広に確保される。これによって、培養液は、十分に確保された空隙Ｓ内を容易に流通することができる。 As described in FIG. 4, a predetermined gap (corresponding to the height I of the spacer 250 in the figure) is secured by the spacer 250 between the inner multi-stage tray 400 and the outer casing 200. In addition, as described in FIG. 6, a meat stealing portion 355 is provided on the outer peripheral surface (side wall 324) of the internal multi-stage tray. As shown in FIG. 7, the meat stealing portion 355 is provided over the entire stacking direction of the internal tray 300, and therefore, in the first communication portion 450, the gap S between the internal multi-stage tray 400 and the external housing 200 is A wider space is secured by the amount of the meat stealing portion 355. As a result, the culture solution can easily circulate in the sufficiently secured space S.
　図８は、図１の第１連通部４５０付近の切欠き拡大図である。図８に示すように、第１連通部４５０においては、上述の肉盗み３５５が幅ｗで内部トレイ３００の積層方向全体にわたって設けられる。これに加えて、図６で述べたように、内部トレイ３００それぞれの側壁３２４に、切欠き部３５１が深さｄで設けられている。内部トレイ３００が積層されることにより、図８に示すように、切欠き部３５１と、直上に積層された内部トレイ３００の内部底面部３１０とに囲まれた第１連通孔ＣＨ１が構成される。同様に、最上段の内部トレイ３００の切欠き部３５１と、上端４２９を覆蓋した蓋体５００とに囲まれた第１連通孔ＣＨ１も構成される。これによって、培養液は、内部トレイ３００内部空間と外部筐体２００の内部空間とを連通する第１連通孔ＣＨ１内を流通することができる。 FIG. 8 is a cutaway enlarged view of the vicinity of the first communication portion 450 in FIG. As shown in FIG. 8, in the first communication portion 450, the meat stealing 355 is provided over the entire stacking direction of the internal tray 300 with a width w. In addition to this, as described in FIG. 6, a notch 351 is provided in the side wall 324 of each inner tray 300 with a depth d. By stacking the inner tray 300, as shown in FIG. 8, the first communication hole CH1 surrounded by the notch portion 351 and the inner bottom surface portion 310 of the inner tray 300 stacked immediately above is formed. . Similarly, the first communication hole CH1 surrounded by the notch 351 of the uppermost internal tray 300 and the lid body 500 covering the upper end 429 is also configured. As a result, the culture solution can circulate in the first communication hole CH1 that connects the internal space of the internal tray 300 and the internal space of the external housing 200.
［第２連通部］
　図１に示すように、第２連通部は、培養フラスコ１００において、ポート２８０近傍に設けられる。本実施形態では、図２に示すように、内部多段トレイ４００を構成する端壁３２１に配設される。第２連通部は、通気のため、および培養液をポート２８０へ送るために利用することができる。したがって、第２連通部は、内部多段トレイ４００を構成する全ての内部トレイ３００の内部空間と、外部筐体２００の内部空間とを連通させるように構成される。 [Second communication section]
As shown in FIG. 1, the second communication portion is provided in the vicinity of the port 280 in the culture flask 100. In the present embodiment, as shown in FIG. 2, it is disposed on the end wall 321 constituting the internal multi-stage tray 400. The second communication portion can be used for aeration and for sending the culture solution to the port 280. Therefore, the second communication unit is configured to communicate between the internal spaces of all the internal trays 300 constituting the internal multistage tray 400 and the internal space of the external housing 200.
　すでに述べたように、内部トレイ３００それぞれの端壁３２１に、切欠き部３８１（図５参照）が深さｄで設けられている。内部トレイ３００が積層されることにより、切欠き部３８１と、直上に積層された内部トレイ３００の内部底面部３１０とに囲まれた第２連通孔ＣＨ２が構成される。同様に、最上段の内部トレイ３００の切欠き部３８１と、上端４２９を覆蓋した蓋体５００とに囲まれた第２連通孔ＣＨ２も構成される。これによって、内容液は、内部トレイ３００の内部空間と外部筐体２００の内部空間とを連通する第２連通孔ＣＨ２内を流通することができる。 As described above, the notch 381 (see FIG. 5) is provided in the end wall 321 of each internal tray 300 at a depth d. By stacking the inner tray 300, a second communication hole CH2 surrounded by the notch 381 and the inner bottom surface 310 of the inner tray 300 stacked immediately above is formed. Similarly, the second communication hole CH2 surrounded by the notch 381 of the uppermost internal tray 300 and the lid 500 covering the upper end 429 is also configured. As a result, the content liquid can flow through the second communication hole CH <b> 2 that communicates the internal space of the internal tray 300 and the internal space of the external housing 200.
［材質および表面処理］
　培養フラスコ１００を構成する外部筐体２００，内部多段トレイ４００および蓋体５００は、熱可塑性樹脂で構成されてよい。好ましくは、ポリスチレンで構成される。
　培養フラスコ１００の構成部材の表面は、当業者によって適宜処理されてよい。たとえば、生物学的薬品（たとえば、細胞外基質、より具体的には、ラミニン、フィブロネクチン、およびコラーゲンの少なくともいずれかの成分）または合成剤による表面処理、若しくは、プラズマ処理等の親水化処理がなされていてよい。 [Material and surface treatment]
The outer casing 200, the inner multi-stage tray 400, and the lid 500 constituting the culture flask 100 may be made of a thermoplastic resin. Preferably, it is made of polystyrene.
The surface of the constituent member of the culture flask 100 may be appropriately treated by those skilled in the art. For example, surface treatment with a biological agent (for example, extracellular matrix, more specifically, at least one component of laminin, fibronectin, and collagen) or a synthetic agent, or hydrophilic treatment such as plasma treatment is performed. It may be.
［培養フラスコの製造方法］
　図９および図１０は、培養フラスコ１００の製造方法の工程を示す模式図である。
　培養フラスコ１００の製造方法においては、まず、図９（i）に示すように、複数（図中では５枚）の内部トレイ３００を用意する。次に図９（ii）に示すように、内部トレイ３００を積層させる。この場合、内部トレイ３００の上端３２９と、別の内部トレイ３００の内部底面部３１０下面とが接触するように内部トレイ３００を重ね、互いに接触する上端３２９と内部底面部３１０とを溶着する。溶着された部分は、液密に封止される。溶着の方法としては、超音波溶接法などが挙げられる。 [Production method of culture flask]
9 and 10 are schematic diagrams showing the steps of the method for manufacturing the culture flask 100.
In the method of manufacturing the culture flask 100, first, as shown in FIG. 9 (i), a plurality of (five in the figure) internal trays 300 are prepared. Next, as shown in FIG. 9 (ii), the internal tray 300 is stacked. In this case, the internal tray 300 is stacked so that the upper end 329 of the internal tray 300 and the lower surface of the internal bottom surface 310 of another internal tray 300 are in contact with each other, and the upper end 329 and the internal bottom surface 310 are in contact with each other. The welded part is sealed in a liquid-tight manner. Examples of the welding method include an ultrasonic welding method.
　溶着された部分は、液密に封止される。これによって、培養空間ｍ２，～，ｍ５が形成される。一方、内部トレイ３００の切欠き部３８１については溶着されることなく、最上段を除いて、切欠き部３８１と直上の内部底面部３１０とに囲まれた第２連通孔ＣＨ２が形成される。図示しない他の切欠き部３５１（図７参照）についても同様に、第１連通孔ＣＨ１が形成される。これにより、内部多段トレイ４００を得る。 The welded part is sealed liquid-tight. Thereby, culture spaces m2, ..., m5 are formed. On the other hand, the notch 381 of the inner tray 300 is not welded, and the second communication hole CH2 surrounded by the notch 381 and the inner bottom surface 310 immediately above is formed except for the uppermost stage. Similarly, the first communication hole CH1 is formed in other notch portions 351 (see FIG. 7) (not shown). Thereby, the internal multi-stage tray 400 is obtained.
　次に、図１０（iii）に示すように、内部多段トレイ４００の上端４２９に、蓋体５００を覆蓋し、上端４２９と蓋体５００の下面とを溶着する。溶着の方法としては、先の工程と同様である。溶着された部分は、液密に封止される。これによって、培養空間ｍ６がさらに形成される。一方、内部多段トレイ４００の最上段に設けられた切欠き部３８１については溶着されることなく、切欠き部３８１と蓋体５００とに囲まれた第２連通孔ＣＨ２が形成される。これによって、第２連通部４８０が形成される。図示しない他の切欠き部３５１（図７参照）についても同様に、最上段における第１連通孔ＣＨ１が形成され、第１連通部４５０が形成される。 Next, as shown in FIG. 10 (iii), the lid 500 is covered with the upper end 429 of the internal multi-stage tray 400, and the upper end 429 and the lower surface of the lid 500 are welded. The welding method is the same as in the previous step. The welded part is sealed in a liquid-tight manner. Thereby, the culture space m6 is further formed. On the other hand, the second communication hole CH2 surrounded by the notch 381 and the lid 500 is formed without welding the notch 381 provided at the uppermost stage of the internal multi-stage tray 400. Thereby, the second communication portion 480 is formed. Similarly, for the other notch 351 (not shown) (see FIG. 7), the first communication hole CH1 in the uppermost stage is formed, and the first communication part 450 is formed.
　次に、図１０（iv）に示すように、蓋体５００が溶着された内部多段トレイ４００を外部筐体２００に収容し、外部筐体２００の上端２２９と蓋体５００の下面とを溶着する。溶着の方法は、先の工程と同様である。これによって、培養空間ｍ１がさらに形成される。適宜、キャップ２９０（図１参照）がポート２８０に装着されることで、培養フラスコ１００を得る。 Next, as shown in FIG. 10 (iv), the inner multi-stage tray 400 with the lid 500 welded is accommodated in the outer casing 200, and the upper end 229 of the outer casing 200 and the lower surface of the lid 500 are welded together. . The welding method is the same as in the previous step. Thereby, the culture space m1 is further formed. The cap 290 (see FIG. 1) is appropriately attached to the port 280 to obtain the culture flask 100.
［操作方法］
　図１１から図１３は、培養フラスコ１００の操作方法の工程を示す説明図である。
　培養フラスコ１００の操作方法においては、まず、培養フラスコ１００内に培養液Ｍを入れ、図１１（I）に示すように、端壁２２２が下となるように立て、静置する。この場合、端壁２２２近傍の側壁３２４に形成された第１連通部４５０を培養液Ｍが流通し、各段に培養液Ｍが分配される。静置時間は、培養液Ｍが第１連通部４５０内を流通することで、各段で液位が同じ高さとなる程度でよく、具体的には１０秒程度である。 [Method of operation]
FIG. 11 to FIG. 13 are explanatory diagrams showing the steps of the method for operating the culture flask 100.
In the operation method of the culture flask 100, first, the culture solution M is put into the culture flask 100, and as shown in FIG. 11 (I), the end wall 222 is placed downward and allowed to stand. In this case, the culture solution M flows through the first communication portion 450 formed on the side wall 324 near the end wall 222, and the culture solution M is distributed to each stage. The standing time may be such that the culture liquid M circulates in the first communication part 450 so that the liquid level becomes the same height in each stage, specifically about 10 seconds.
　次に、図１１（II）に示すように、培養フラスコ１００を、第１連通部４５０が形成されていない側（外部筐体の側壁２２３側）に傾け、第１連通部４５０から培養液Ｍを隔離する。培養フラスコ１００を傾ける角度θは、たとえば４５度以上７０度以下である。
　第１連通部から培養液Ｍを隔離した状態を維持するため、角度θをおおよそ保った状態で、図１２（III）に示すように、外部底面部２１０が下となるように培養フラスコ１００を倒す。その結果、図１２（IV）に示すように、培養フラスコの各段に、均等に培養液Ｍが分配される。 Next, as shown in FIG. 11 (II), the culture flask 100 is tilted to the side where the first communication portion 450 is not formed (side wall 223 side of the external housing), and the culture solution M is transferred from the first communication portion 450. Isolate. The angle θ at which the culture flask 100 is tilted is, for example, not less than 45 degrees and not more than 70 degrees.
In order to maintain the state in which the culture medium M is isolated from the first communication portion, the culture flask 100 is placed so that the external bottom surface portion 210 is at the bottom as shown in FIG. knock down. As a result, as shown in FIG. 12 (IV), the culture solution M is evenly distributed to each stage of the culture flask.
　培養液Ｍを培養フラスコ外に排出する場合は、図１３（V）に記載のように、ポート２８０からデカンテーションによって排出操作を行う。この場合、内部多段トレイ４００の各内部トレイ３００内の培養液Ｍが、第２連通部４８０の第２連通孔ＣＨ２を通って外部筐体２００内に排出される（図３参照）。さらに、培養液Ｍは、外部筐体２００の第１傾斜壁２２５（または第１傾斜壁２２６）および第２傾斜壁２２７（または第２傾斜壁２２８）内周面を伝ってポート２８０から外部へ排出される。 When the culture solution M is discharged out of the culture flask, as shown in FIG. In this case, the culture solution M in each internal tray 300 of the internal multi-stage tray 400 is discharged into the external housing 200 through the second communication hole CH2 of the second communication portion 480 (see FIG. 3). Further, the culture medium M travels from the port 280 to the outside through the inner peripheral surface of the first inclined wall 225 (or the first inclined wall 226) and the second inclined wall 227 (or the second inclined wall 228) of the outer casing 200. Discharged.
［他の例］
　図１４から図２３は、培養フラスコ１００の他の例を示す。他の例においては、主に第１実施形態と異なる点について説明し、同一点については説明を省略する。 [Other examples]
14 to 23 show another example of the culture flask 100. In other examples, differences from the first embodiment will be mainly described, and descriptions of the same points will be omitted.
［第１連通部の他の例］
　図１４は、培養フラスコ１００ａの第１連通部４５０ａ付近の切欠き拡大図であり、第１実施形態の図８に対応する。図１５は、図１４の線Ｂａにおける断面図であり、図１６は、図１４の線Ｃａにおける断面図である。図１５および図１６は、それぞれ、第１実施形態の図４および図７に対応する。 [Other examples of the first communication part]
FIG. 14 is an enlarged view of a cutout in the vicinity of the first communication portion 450a of the culture flask 100a, and corresponds to FIG. 8 of the first embodiment. 15 is a cross-sectional view taken along line Ba in FIG. 14, and FIG. 16 is a cross-sectional view taken along line Ca in FIG. 15 and 16 correspond to FIGS. 4 and 7 of the first embodiment, respectively.
　図１４および図１５に示すように、培養フラスコ１００ａは、外部筐体２００ａにおいて保持部２３０ａを有する一方、スペーサを有しない。さらに、内部多段トレイ４００ａの側壁３２４ａにおいて、切欠き部３５１ａと肉盗み部３５５ａを有する。切欠き部３５１ａおよび肉盗み部３５５ａは、第１実施形態よりも幅広の幅ｗａで設けられている。 As shown in FIGS. 14 and 15, the culture flask 100a has a holding portion 230a in the outer casing 200a, but does not have a spacer. Further, the side wall 324a of the internal multi-stage tray 400a has a cutout portion 351a and a meat stealing portion 355a. The cutout portion 351a and the meat stealing portion 355a are provided with a width wa wider than that in the first embodiment.
　内部多段トレイ４００ａは、第１実施形態の内部多段トレイ４００と同様に製造される。ここで、内部トレイ３００ａが積層される工程では（特に超音波溶着法が用いられた場合）、内部トレイ３００ａの積層ずれが生じ得る。積層ずれが生じると、図１５に示すように、内部多段トレイ４００ａにおける側壁３２４ａと外部筐体２００ａの側壁２２４ａとの間の空隙の幅は一定にならない。 The internal multi-stage tray 400a is manufactured in the same manner as the internal multi-stage tray 400 of the first embodiment. Here, in the process of stacking the inner tray 300a (particularly when the ultrasonic welding method is used), the stacking deviation of the inner tray 300a may occur. When the stacking deviation occurs, as shown in FIG. 15, the width of the gap between the side wall 324a of the inner multi-stage tray 400a and the side wall 224a of the outer casing 200a is not constant.
　このような積層ずれの場合、図１５に示すように、最下段の内部トレイ３００ａの側壁３２４ａが、外部筐体２００ａの側壁２２４ａとほとんど接触する位にまで近接している。このため、最下段の内部トレイ３００ａでは、側壁３２４ａと側壁２２４ａとの間には十分な空隙は確保されない。このような場合において、もし、連通を十分に確保する構造が他になかった場合、側壁３２４ａと側壁２２４ａとの間で流通障害が起こり、内部底面部４１０ａより下の培養空間ｍ１まで培養液が容易に分配されない恐れがある。 In the case of such a stacking deviation, as shown in FIG. 15, the side wall 324a of the lowermost internal tray 300a is close enough to come into contact with the side wall 224a of the outer casing 200a. For this reason, in the lowermost internal tray 300a, a sufficient gap is not secured between the side wall 324a and the side wall 224a. In such a case, if there is no other structure that ensures sufficient communication, a flow failure occurs between the side wall 324a and the side wall 224a, and the culture solution flows to the culture space m1 below the inner bottom surface portion 410a. There is a risk of not being easily distributed.
　しかしながら、図１４および図１６に示すように、側壁２２４ａには肉盗み部３５５ａが設けられている。このため、第１連通部４５０ａにおいては、最下段の内部トレイ３００ａの部分であっても、肉盗み部３５５ａと側壁２２４ａとの間に空隙Ｓａ（図１６参照）が確保される。したがって、図１５のような積層ずれがある場合であっても、培養フラスコ１００ａの最下段の培養空間ｍ１まで培養液を容易に分配することができる。 However, as shown in FIGS. 14 and 16, the side wall 224a is provided with a meat stealing portion 355a. Therefore, in the first communication portion 450a, a gap Sa (see FIG. 16) is secured between the meat stealing portion 355a and the side wall 224a even in the lowermost internal tray 300a. Therefore, even when there is a stacking deviation as shown in FIG. 15, the culture solution can be easily distributed to the lowest culture space m1 of the culture flask 100a.
［第１連通部のさらに他の例］
　図１７は、培養フラスコ１００ｂの第１連通部４５０ｂ付近の切欠き拡大図であり、第１実施形態の図８に対応する。図１８は、図１７の線Ｂｂにおける断面図であり、図１９は、図１７の線Ｃｂにおける断面図である。図１８および図１９は、それぞれ、第１実施形態の図４および図７に対応する。 [Further example of the first communication part]
FIG. 17 is an enlarged view of a cutout in the vicinity of the first communication portion 450b of the culture flask 100b, and corresponds to FIG. 8 of the first embodiment. 18 is a cross-sectional view taken along line Bb in FIG. 17, and FIG. 19 is a cross-sectional view taken along line Cb in FIG. 18 and 19 correspond to FIGS. 4 and 7 of the first embodiment, respectively.
　図１７および図１８に示すように、培養フラスコ１００ｂは、外部筐体２００ｂにおいて保持部２３０ｂとスペーサ２５０ｂとを有する。内部多段トレイ４００ｂにおいては、切欠き部３５１ｂを有する一方、肉盗み部を有さない。 As shown in FIGS. 17 and 18, the culture flask 100b has a holding part 230b and a spacer 250b in the outer casing 200b. The internal multi-stage tray 400b has a cutout portion 351b, but does not have a meat stealing portion.
　ここで、内部多段トレイ４００ｂには、上記と同様に、積層ずれが生じ得る。このような積層ずれの場合、図１８に示すように、最下段の内部トレイ３００ｂの側壁３２４ｂが外部筐体２００ｂの側壁２２４ｂに最も接近する。しかしながら、図１８に示すように、側壁２２４ｂから突設されたスペーサ２５０ｂが、側壁２２４ｂと最下段の内部トレイ３００ｂの側壁３２４ｂとの間に介在することにより、図１９に示すように、最も接近する側壁３２４ｂと側壁２２４ｂとの間であっても、十分な空隙Ｓｂが確保される。したがって、図１８のような積層ずれがある場合であっても、培養フラスコ１００ｂの最下段の培養空間ｍ１まで培養液を容易に分配することができる。 Here, the internal multi-stage tray 400b may be misaligned as described above. In the case of such a stacking deviation, as shown in FIG. 18, the side wall 324b of the lowermost internal tray 300b is closest to the side wall 224b of the external housing 200b. However, as shown in FIG. 18, the spacer 250b protruding from the side wall 224b is interposed between the side wall 224b and the side wall 324b of the inner tray 300b at the lowest stage, so that the closest approach is shown in FIG. Even between the side wall 324b and the side wall 224b, sufficient gap Sb is secured. Therefore, even when there is a stacking deviation as shown in FIG. 18, the culture solution can be easily distributed to the lowermost culture space m1 of the culture flask 100b.
　なお、本発明の培養フラスコが、内部多段トレイの積層ずれがあっても培養液の流通障害を回避できることは、図１４から図１９で説明した他の態様における場合に限られるものではない。流通障害の回避効果の観点からは、スペーサ２５０および肉盗み部３５５の両方を具備する第１実施形態において当該効果をより有利に得られることは、言うまでもない。 It should be noted that the fact that the culture flask of the present invention can avoid the flow of the culture solution even when the internal multistage tray is misaligned is not limited to the case of the other modes described with reference to FIGS. From the viewpoint of the effect of avoiding the distribution trouble, it goes without saying that the effect can be obtained more advantageously in the first embodiment including both the spacer 250 and the meat stealing portion 355.
［第１連通部のさらに他の例］
　図２０および図２１は、内部トレイ３００の側壁３２４に設けられた切欠き部３５１および肉盗み部３５５の他の例を示す。図２０および図２１は、第１実施形態の図６に対応する。 [Further example of the first communication part]
20 and 21 show other examples of the notch portion 351 and the meat stealing portion 355 provided on the side wall 324 of the internal tray 300. FIG. 20 and 21 correspond to FIG. 6 of the first embodiment.
　図２０に示すように、側壁３２４ｃにおいては、切欠き部３５１ｃが、肉盗み部３５５ｃの幅ｗよりも広い幅ｗｃで設けられてもよい。また、図２１に示すように、側壁３２４ｄにおいては、肉盗み部３５５ｄが、切欠き部３５１ｄの幅ｗよりも広い幅ｗｄで設けられてもよい。 As shown in FIG. 20, in the side wall 324c, the notch portion 351c may be provided with a width wc wider than the width w of the meat stealing portion 355c. In addition, as shown in FIG. 21, in the side wall 324d, the meat stealing portion 355d may be provided with a width wd wider than the width w of the notch portion 351d.
［第１連通部のさらに他の例］
　第１実施形態においては、スペーサ２５０が第１連通部４５０よりポート２８０側の近傍に配設されている（図１参照）が、ポート２８０とは反対側の近傍に配設されてもよい。スペーサ２５０は、本実施形態では保持部２３０の上に連設されている（図２参照）が、保持部２３０とスペーサ２５０とは、分離されて設けられてもよい。スペーサ２５０の上下方向の長さは、本実施形態では内部トレイ３００一段分の高さである（図４参照）が、任意の高さであってよい。たとえば、スペーサ２５０は、外部筐体２００の深さ（外部立ち上がり周壁部２２０内周面の上下方向の長さ）全部に延在するように配設されてもよい。 [Further example of the first communication part]
In the first embodiment, the spacer 250 is disposed in the vicinity of the port 280 side of the first communication portion 450 (see FIG. 1), but may be disposed in the vicinity of the opposite side of the port 280. In this embodiment, the spacer 250 is continuously provided on the holding unit 230 (see FIG. 2), but the holding unit 230 and the spacer 250 may be provided separately. The vertical length of the spacer 250 is the height of one stage of the inner tray 300 in this embodiment (see FIG. 4), but may be any height. For example, the spacer 250 may be disposed so as to extend to the entire depth of the external casing 200 (the vertical length of the inner peripheral surface of the external rising peripheral wall 220).
［外部筐体の他の例］
　図２２および図２３は、外部筐体２００の他の例を示す。
　図２２に示すように、外部筐体２００ｅにおいては、傾斜壁２２５ｅ，２２６ｅが、それぞれ側壁２２３ｅ，２２４ｅから直接端壁２２１ｅに連設されていてもよい。
　また、図２３に示すように、外部筐体２００ｆにおいては、傾斜壁２２５ｆ，２２６ｆが、それぞれ側壁２２３ｆ，２２４ｆから直接端壁２２１ｆに連設され、かつ、下部傾斜壁２１１ｆが、外部底面部２１０ｆから端壁２２１ｆに連設されていてもよい。この場合、下部傾斜壁２１１ｆには、脚部２１５ｆが突設されていてもよい。 [Other examples of external enclosure]
22 and 23 show another example of the external housing 200. FIG.
As shown in FIG. 22, in the external housing 200e, the inclined walls 225e and 226e may be connected to the end wall 221e directly from the side walls 223e and 224e, respectively.
Further, as shown in FIG. 23, in the external housing 200f, the inclined walls 225f and 226f are connected to the end wall 221f directly from the side walls 223f and 224f, respectively, and the lower inclined wall 211f is connected to the outer bottom surface portion 210f. To the end wall 221f. In this case, leg portions 215f may protrude from the lower inclined wall 211f.
［外部筐体のさらに他の例］
　第１実施形態においては、保持部２３０が側壁２２３，２２４に設けられているが、この態様に限定されるものではない。保持部２３０は、内部底面部４１０が外部底面部２１０と平行かつ所定の高さとなるように内部多段トレイ４００を保持する態様であれば、その数および配設箇所は限定されない。したがって、同様の保持部２３０が、外部立ち上がり周壁部２２０の内周面のいずれの場所に設けられてもよい。 [Still other examples of external housing]
In 1st Embodiment, although the holding | maintenance part 230 is provided in the side walls 223 and 224, it is not limited to this aspect. The number and arrangement locations of the holding portions 230 are not limited as long as the holding portions 230 hold the inner multi-stage tray 400 so that the inner bottom portion 410 is parallel to the outer bottom portion 210 and has a predetermined height. Therefore, the same holding | maintenance part 230 may be provided in any place of the internal peripheral surface of the external standing surrounding wall part 220. FIG.
［外部筐体のさらに他の例］
　第１実施形態では、ポート２８０が、その軸心が側壁２２３，２２４間の中央に位置するように設けられているが、この態様に限定されることなく、ポート２８０は、その軸心が側壁２２３，２２４間の中央からずれた位置となるように設けられてもよい。さらに、第１実施形態では、ポート２８０の軸心が、外部筐体２００の長手方向に平行であるように設けられているが、この態様に限定されることなく、当該長手方向に非平行であってもよい。ポート２８０の位置および形状は、たとえばデカンテーション（図１１（V）参照）の観点から、当業者によって適宜設計されてよい。 [Still other examples of external housing]
In the first embodiment, the port 280 is provided such that its axis is located at the center between the side walls 223 and 224. However, the port 280 is not limited to this aspect, and the axis of the port 280 is the side wall. 223 and 224 may be provided so as to deviate from the center. Further, in the first embodiment, the axis of the port 280 is provided so as to be parallel to the longitudinal direction of the external housing 200, but is not limited to this aspect, and is not parallel to the longitudinal direction. There may be. The position and shape of the port 280 may be appropriately designed by those skilled in the art from the viewpoint of, for example, decantation (see FIG. 11 (V)).
［第１実施形態および他の例によって得られる効果］
　以上のように、細胞培養フラスコ１００，１００ａ，１００ｂは、簡素な構成でありながら、最上段の内部トレイ３００，３００ａ，３００ｂに収容された液体が毛管現象で不所望に漏れ出すことを防止することができる。このため、たとえば内部トレイ３００，３００ａ，３００ｂの内部立ち上がり周壁部３２０（側壁３２４，３２４ａ，３２４ｂ，３２４ｃ，３２４ｄ）の高さｈを小さくし、外部筐体２００，２００ａ，２００ｂ，２００ｅ，２００ｆの深さの割に内部トレイ３００，３００ａ，３００ｂの積層段数を多くして、培養効率を向上させることができる。さらに、内部多段トレイ４００，４００ａ，４００ｂが外部筐体２００，２００ａ，２００ｂ，２００ｅ，２００ｆに収容されているため、たとえ内部多段トレイ４００，４００ａ，４００ｂの積層ずれがあったとしても外部寸法（外部筐体２００，２００ａ，２００ｂ，２００ｅ，２００ｆの寸法に相当）の製品個体差の発生を排除することができ、製造工程でのマシンによるハンドリングが容易となる。 [Effects obtained by the first embodiment and other examples]
As described above, the cell culture flasks 100, 100a, 100b have a simple configuration, but prevent liquid stored in the uppermost internal trays 300, 300a, 300b from undesirably leaking due to capillary action. be able to. For this reason, for example, the height h of the internal rising peripheral wall portion 320 ( side walls 324, 324a, 324b, 324c, 324d) of the internal trays 300, 300a, 300b is reduced so that the external casings 200, 200a, 200b, 200e, 200f The culture efficiency can be improved by increasing the number of stacked layers of the internal trays 300, 300a, and 300b for the depth. Further, since the internal multi-stage trays 400, 400a, 400b are accommodated in the external housings 200, 200a, 200b, 200e, 200f, even if there is a stacking shift of the internal multi-stage trays 400, 400a, 400b, external dimensions ( The occurrence of individual product differences (corresponding to the dimensions of the external casings 200, 200a, 200b, 200e, and 200f) can be eliminated, and handling by the machine in the manufacturing process is facilitated.
　細胞培養フラスコ１００，１００ａは、内部立ち上がり周壁部３２０（側壁３２４，３２４ａ，３２４ｃ，３２４ｄ）の外周側において、第１連通孔ＣＨ１，ＣＨ１ａ，ＣＨ１ｂと連通する肉盗み部３５５，３５５ａ，３５５ｃ，３５５ｄが、内部トレイ３００，３００ａの積層方向全体にわたって形成されているため、第１連通孔ＣＨ１，ＣＨ１ａ内の液流がよりスムーズとなる。さらに、肉盗み部３５５，３５５ａ，３５５ｃ，３５５ｄが内部立ち上がり周壁部３２０（側壁３２４，３２４ａ，３２４ｂ，３２４ｃ，３２４ｄ）に設けられており、内部底面部３１０に連通のための孔を有しないため培養容積のロスが無い。したがって、培養容積を効率よく確保することができる。 The cell culture flasks 100 and 100a have meat stealing portions 355, 355a, 355c, and 355d that communicate with the first communication holes CH1, CH1a, and CH1b on the outer peripheral side of the inner rising peripheral wall portion 320 ( side walls 324, 324a, 324c, and 324d). However, since the inner trays 300 and 300a are formed over the entire stacking direction, the liquid flow in the first communication holes CH1 and CH1a becomes smoother. Further, the meat stealing portions 355, 355a, 355c, and 355d are provided on the inner rising peripheral wall portion 320 ( side walls 324, 324a, 324b, 324c, and 324d), and the inner bottom surface portion 310 does not have a hole for communication. There is no loss of culture volume. Therefore, the culture volume can be secured efficiently.
　細胞培養フラスコ１００，１００ｂは、外部立ち上がり周壁部２２０（側壁２２４，２２４ｂ）の内周側において、内部立ち上がり周壁部３２０（側壁３２４，３２４ｂ）の第１連通孔ＣＨ１，ＣＨ１ｂ近傍に接触するようにスペーサ２５０，２５０ｂが突設されているため、第１連通孔ＣＨ１，ＣＨ１ｂ内の液流がよりスムーズとなる。 The cell culture flasks 100 and 100b are in contact with the vicinity of the first communication holes CH1 and CH1b of the internal rising peripheral wall 320 ( side walls 324 and 324b) on the inner peripheral side of the external rising peripheral wall 220 ( side walls 224 and 224b). Since the spacers 250 and 250b are protruded, the liquid flow in the first communication holes CH1 and CH1b becomes smoother.
　細胞培養フラスコ１００，１００ａ，１００ｂは、外部立ち上がり周壁部２２０（側壁２２４，２２４ａ，２２４ｂ）の内周側において、内部多段トレイ４００，４００ａ，４００ｂを外部底面部２１０，２１０ａ，２１０ｂから所定の高さで離間させて保持する保持部２３０，２３０ａ，２３０ｂが突設されているため、外部底面部２１０，２１０ａ，２１０ｂも培養空間ｍ１として利用することができる。 The cell culture flasks 100, 100a, 100b are arranged so that the inner multi-stage trays 400, 400a, 400b are placed at a predetermined height from the outer bottom surface portions 210, 210a, 210b on the inner peripheral side of the outer rising peripheral wall portion 220 ( side walls 224, 224a, 224b). Since the holding portions 230, 230a, and 230b that are separated and held are protruded, the external bottom surface portions 210, 210a, and 210b can also be used as the culture space m1.
　細胞培養フラスコ１００は、外部筐体２００の長手方向に対向する外部立ち上がり周壁部２２０（端壁２２１，２２２）間の長さが、外部筐体２００の短手方向に対向する外部立ち上がり周壁部２２０（側壁２２３，２２４）間の長さの１．９倍以上２．２倍以下であるため、多段フラスコでありながらも、片手での持ち運びがしやすい。本発明の細胞培養フラスコ１００は、内部トレイ３００からの不所望の液体漏れを防止できるため、傾きの影響を受けやすいこのような長タイプの形状も許容される。また、長タイプであることによって、たとえば、内部トレイ３００の１段当たりの容量の割に当該１段当たりの深さを小さくしても培養容積を充分確保することができるため、外部筐体２００の深さの割に積層段数をより多くすることができ、したがって、培養効率をより向上させることができる。 In the cell culture flask 100, the length between the outer rising peripheral wall portions 220 (end walls 221, 222) facing the longitudinal direction of the outer casing 200 is the outer rising peripheral wall portion 220 facing the shorter direction of the outer casing 200. Since it is 1.9 times or more and 2.2 times or less of the length between (side walls 223, 224), it is easy to carry with one hand even though it is a multistage flask. Since the cell culture flask 100 of the present invention can prevent undesired liquid leakage from the inner tray 300, such a long type shape that is easily affected by inclination is also allowed. In addition, because of the long type, for example, a sufficient culture volume can be secured even if the depth per stage is reduced relative to the capacity per stage of the internal tray 300. Therefore, the number of stacked layers can be increased for the depth of the cell, and thus the culture efficiency can be further improved.
　細胞培養フラスコ１００，１００ａ，１００ｂは、内部トレイ３００，３００ａ，３００ｂの内部底面部の厚みが１．２ｍｍ以上２．０ｍｍ以下であるため、内部トレイ３００，３００ａ，３００ｂの１段当たりの容量の割に、内部立ち上がり周壁部３２０（側壁３２４，３２４ａ，３２４ｂ，３２４ｃ，３２４ｄ）の高さｈを小さくすることができる。このため、外部筐体２００の深さの割に積層段数をより多くすることができ、したがって、培養効率をより向上させることができる。
　細胞培養フラスコ１００，１００ａ，１００ｂは、内部多段トレイ４００，４００ａ，４００ｂが外部筐体２００，２００ａ，２００ｂに収容されているため、内部多段トレイ４００，４００ａ，４００ｂの内部底面部の厚みが相対的に小さくても、細胞培養フラスコ１００，１００ａ，１００ｂ全体としての強度が保証される。 In the cell culture flasks 100, 100a, 100b, the inner bottom surfaces of the inner trays 300, 300a, 300b have a thickness of 1.2 mm or more and 2.0 mm or less, so that the capacity of the inner trays 300, 300a, 300b can be reduced. However, the height h of the internal rising peripheral wall 320 ( side walls 324, 324a, 324b, 324c, 324d) can be reduced. For this reason, it is possible to increase the number of stacking stages for the depth of the outer casing 200, and thus it is possible to further improve the culture efficiency.
In the cell culture flasks 100, 100a, 100b, since the inner multi-stage trays 400, 400a, 400b are accommodated in the outer casings 200, 200a, 200b, the thickness of the inner bottom surface of the inner multi-stage trays 400, 400a, 400b is relatively Even if it is small, the strength of the entire cell culture flask 100, 100a, 100b is guaranteed.
　細胞培養フラスコ１００，１００ａ，１００ｂにおいては、内部トレイ３００，３００ａ，３００ｂの内部立ち上がり周壁部３２０（側壁３２４，３２４ａ，３２４ｂ，３２４ｃ，３２４ｄ）の高さｈが８．５ｍｍ以上１１．０ｍｍ以下であるため、外部筐体２００，２００ａ，２００ｂの深さの割に積層段数をより多くすることができ、培養効率をより向上させることができる。 In the cell culture flasks 100, 100a, 100b, the height h of the internal rising peripheral wall 320 ( side walls 324, 324a, 324b, 324c, 324d) of the internal trays 300, 300a, 300b is 8.5 mm or more and 11.0 mm or less. Therefore, the number of stacked layers can be increased for the depth of the outer casings 200, 200a, and 200b, and the culture efficiency can be further improved.
　細胞培養フラスコ１００においては、外部立ち上がり周壁部２２０において、第１傾斜壁２２５，２２６および第２傾斜壁２２７，２２８を含み、内部多段トレイ４００における内部立ち上がり周壁部３２０は、外部立ち上がり周壁部２２０のうち、側壁２２３，２２４、端壁２２２、および第１傾斜壁２２５，２２６に沿うように形成されるため、第２傾斜壁２２７，２２８によってポート２８０からのデカンテーションを容易にしつつも、第１傾斜壁２２５，２２６によって、１段あたりの培養容積をより大きくとることができる。 The cell culture flask 100 includes first inclined walls 225 and 226 and second inclined walls 227 and 228 in the outer rising peripheral wall portion 220, and the inner rising peripheral wall portion 320 in the internal multi-stage tray 400 is the outer rising peripheral wall portion 220. Of these, the first inclined walls 227 and 228 are formed along the side walls 223 and 224, the end wall 222, and the first inclined walls 225 and 226. By the inclined walls 225 and 226, the culture volume per stage can be increased.
［第１実施形態および他の例における各部と請求項の各構成要素との対応関係］
　本発明においては、細胞培養フラスコ１００，１００ａ，１００ｂが「細胞培養フラスコ」に相当し、外部底面部２１０，２１０ａ，２１０ｂ，２１０ｆが「外部底面部」に相当し、外部立ち上がり周壁部２２０が「外部立ち上がり周壁部」に相当し、ポート２８０が「ポート」に相当し、外部筐体２００，２００ａ，２００ｂ，２００ｅ，２００ｆが「外部筐体」に相当し、内部底面部３１０が「内部底面部」に相当し、内部立ち上がり周壁部３２０が「内部立ち上がり周壁部」に相当し、内部トレイ３００，３００ａ，３００ｂが「内部トレイ」に相当し、上端３２９が「内部立ち上がり周壁部の上端」に相当し、内部多段トレイ４００，４００ａ，４００ｂが「内部多段トレイ」に相当し、上端２２９が「外部立ち上がり周壁部の上端」に相当し、上端４２９が「最上段内部トレイの内部立ち上がり周壁部の上端」に相当し、蓋体５００が「蓋体」に相当し、第１連通孔ＣＨ１，ＣＨ１ａ，ＣＨ１ｂが「連通孔」に相当し、肉盗み部３５５，３５５ａ，３５５ｃ，３５５ｄが「肉盗み部」に相当し、スペーサ２５０，２５０ｂが「スペーサ」に相当し、高さＨが「所定の高さ」に相当し、保持部２３０，２３０ａ，２３０ｂが「保持部」に相当し、高さｈが「内部立ち上がり周壁部の高さ」に相当し、側壁２２３，２２４，２２４ａ，２２４ｂが「側壁」に相当し、端壁２２１，２２２，２２１ｅ，２２１ｆが「端壁」に相当し、第１傾斜壁２２５，２２６が「第１傾斜側壁」に相当し、第２傾斜壁２２７，２２８が「第２傾斜側壁」に相当し、切欠き部３５１，３５１ａ，３５１ｂ，３５１ｃ，３５１ｄが「切欠き部」に相当する。 [Correspondence between Each Part in First Embodiment and Other Examples and Each Component in Claim]
In the present invention, the cell culture flasks 100, 100a, 100b correspond to “cell culture flasks”, the external bottom surface portions 210, 210a, 210b, 210f correspond to “external bottom surface portions”, and the external rising peripheral wall portions 220 are “ Corresponding to “external rising peripheral wall portion”, port 280 corresponds to “port”, external housings 200, 200a, 200b, 200e, 200f correspond to “external housing”, and internal bottom surface portion 310 corresponds to “internal bottom surface portion”. The internal rising peripheral wall 320 corresponds to “internal rising peripheral wall”, the internal trays 300, 300a, 300b correspond to “internal tray”, and the upper end 329 corresponds to “upper end of the internal rising peripheral wall”. The inner multi-stage trays 400, 400a, 400b correspond to “inner multi-stage trays”, and the upper end 229 is “the upper end of the external rising peripheral wall portion”. The upper end 429 corresponds to “the upper end of the internal rising peripheral wall portion of the uppermost internal tray”, the lid 500 corresponds to the “lid”, and the first communication holes CH1, CH1a, CH1b are “communication holes”. The meat stealing portions 355, 355a, 355c, and 355d correspond to “meat stealing portions”, the spacers 250 and 250b correspond to “spacers”, the height H corresponds to “predetermined height”, The holding portions 230, 230a, and 230b correspond to the “holding portion”, the height h corresponds to the “height of the internal rising peripheral wall portion”, the side walls 223, 224, 224a, and 224b correspond to the “side wall”, The walls 221, 222, 221e, 221f correspond to “end walls”, the first inclined walls 225, 226 correspond to “first inclined side walls”, and the second inclined walls 227, 228 become “second inclined side walls”. Corresponding to the notches 351, 351a, 51b, 351c, 351d is equivalent to "the notch".
　本発明の好ましい実施形態は上記の通りであるが、本発明はそれらのみに限定されるものではなく、本発明の趣旨と範囲とから逸脱することのない様々な実施形態が他になされる。さらに、本実施形態において述べられる作用および効果は一例であり、本発明を限定するものではない。 Preferred embodiments of the present invention are as described above, but the present invention is not limited to them, and various other embodiments can be made without departing from the spirit and scope of the present invention. Furthermore, the operations and effects described in this embodiment are merely examples, and do not limit the present invention.

Claims (9)

  1. 　外部底面部と、前記外部底面部の全周に設けられた外部立ち上がり周壁部と、前記外部立ち上がり周壁部の一部に設けられたポートとを含む外部筐体と、
    　内部底面部と、前記内部底面部の全周に設けられた内部立ち上がり周壁部とを含む内部トレイが複数段積層され、前記内部立ち上がり周壁部の上端と、上記上端に積重なる前記内部底面部の下面とが互いに溶着され、且つ、前記外部筐体内に収容された内部多段トレイと、
    　前記外部立ち上がり周壁部の上端と、前記内部多段トレイにおける最上段内部トレイの前記内部立ち上がり周壁部の上端との両方を被覆し、且つ、前記両方の上端と溶着する蓋体と、
    　前記内部多段トレイの各段における前記内部立ち上がり周壁部に、前記内部トレイ内の空間と前記外部筐体内の空間とを連通させる連通孔が形成されている、細胞培養フラスコ。     An external casing including an external bottom surface portion, an external rising peripheral wall portion provided on the entire circumference of the external bottom surface portion, and a port provided in a part of the external rising peripheral wall portion;
    A plurality of internal trays including an inner bottom surface portion and an inner rising peripheral wall portion provided on the entire circumference of the inner bottom surface portion are stacked, and the upper end of the inner rising peripheral wall portion and the inner bottom surface portion stacked on the upper end are stacked. An inner multi-stage tray whose lower surface is welded to each other and housed in the outer casing;
    Covering both the upper end of the outer rising peripheral wall portion and the upper end of the inner rising peripheral wall portion of the uppermost internal tray in the internal multi-stage tray, and a lid body that is welded to both upper ends;
    A cell culture flask in which a communication hole for communicating the space in the internal tray and the space in the external housing is formed in the internal rising peripheral wall portion in each stage of the internal multi-stage tray.
  2. 　前記内部立ち上がり周壁部の外周側において、前記連通孔と連通する肉盗み部が、前記内部トレイの積層方向全体にわたって形成されている、請求項１に記載の細胞培養フラスコ。 The cell culture flask according to claim 1, wherein a meat stealing portion communicating with the communication hole is formed over the entire stacking direction of the internal tray on the outer peripheral side of the internal rising peripheral wall portion.
  3. 　前記外部立ち上がり周壁部の内周側において、前記内部立ち上がり周壁部の前記連通孔近傍に接触するようにスペーサが突設される、請求項１または２に記載の細胞培養フラスコ。 The cell culture flask according to claim 1 or 2, wherein a spacer projects from the inner peripheral side of the outer rising peripheral wall portion so as to contact the vicinity of the communication hole of the inner rising peripheral wall portion.
  4. 　前記外部立ち上がり周壁部の内周側において、前記内部多段トレイを前記外部底面部から所定の高さで離間させて保持する保持部が突設され、且つ、前記保持部が前記スペーサに連設される、請求項３に記載の細胞培養フラスコ。 On the inner peripheral side of the outer rising peripheral wall portion, a holding portion that holds the inner multi-stage tray spaced apart from the outer bottom surface portion at a predetermined height protrudes, and the holding portion is connected to the spacer. The cell culture flask according to claim 3.
  5. 　前記外部筐体の長手方向に対向する前記外部立ち上がり周壁部間の長さが、前記外部筐体の短手方向に対向する前記外部立ち上がり周壁部間の長さの１．９倍以上２．２倍以下である、請求項１から４のいずれか１項に記載の細胞培養フラスコ。 The length between the external rising peripheral wall portions facing in the longitudinal direction of the external casing is 1.9 times or more of the length between the external rising peripheral wall portions facing in the short direction of the external casing. The cell culture flask according to any one of claims 1 to 4, wherein the cell culture flask is twice or less.
  6. 　前記内部底面部の厚みが、１．２ｍｍ以上２．０ｍｍ以下である、請求項１から５のいずれか１項に記載の細胞培養フラスコ。 The cell culture flask according to any one of claims 1 to 5, wherein the inner bottom surface has a thickness of 1.2 mm or more and 2.0 mm or less.
  7. 　前記内部立ち上がり周壁部の高さが、８．５ｍｍ以上１１．０ｍｍ以下である、請求項１から６のいずれか１項に記載の細胞培養フラスコ。 The cell culture flask according to any one of claims 1 to 6, wherein a height of the inner rising peripheral wall portion is 8.5 mm or more and 11.0 mm or less.
  8. 　前記外部立ち上がり周壁部が、対向する一対の側壁と、一方に前記ポートが形成された対向する一対の端壁と、前記一対の側壁の少なくとも一方から延び且つ上方からの平面視において前記ポートの根元に向かって傾斜する傾斜側壁を含み、
    　前記傾斜側壁が、前記側壁から延び且つ前記ポートの軸心に対してより小さい角度で傾斜する第１傾斜側壁と、前記第１傾斜側壁から延びかつ前記軸心に対してより大きい角度で傾斜する第２傾斜側壁とから構成され、
    　前記内部立ち上がり周壁部が、前記側壁、前記端壁の他方、および前記第１傾斜側壁に沿うように形成される、請求項１から７のいずれか１項に記載の細胞培養フラスコ。     The outer rising peripheral wall portion includes a pair of opposed side walls, a pair of opposed end walls formed with the port on one side, and extends from at least one of the pair of side walls, and in the plan view from above, the root of the port Including inclined side walls inclined toward the
    The inclined sidewall extends from the sidewall and is inclined at a smaller angle with respect to the axis of the port; and the inclined sidewall extends from the first inclined sidewall and is inclined at a larger angle with respect to the axis. A second inclined side wall,
    The cell culture flask according to any one of claims 1 to 7, wherein the inner rising peripheral wall portion is formed along the side wall, the other of the end walls, and the first inclined side wall.
  9. 　内部底面部と、前記内部底面部の全周に設けられた内部立ち上がり周壁部と、前記内部立ち上がり周壁部の上端の一部が切欠かれた切欠き部とを含む内部トレイを、複数積層し、前記内部立ち上がり周壁部の上端と、上記上端に積重なる前記内部底面部の下面とを互いに溶着し、内部多段トレイを得る積層工程と、
    　前記内部多段トレイにおける最上段内部トレイの前記内部立ち上がり周壁部の上端を蓋体で被覆し、前記上端と蓋体とを溶着し、覆蓋された内部多段トレイを得る覆蓋工程と、　外部底面部と、前記外部底面部の全周に設けられた外部立ち上がり周壁部と、前記外部立ち上がり周壁部の一部に設けられたポートとを含む外部筐体の内部に、前記覆蓋された内部多段トレイを収容し、前記外部立ち上がり周壁部の上端と前記蓋体とを溶着し、内部多段トレイ収容筐体を得る収容工程と、を含む、細胞培養フラスコの製造方法。     A plurality of internal trays including an inner bottom surface portion, an inner rising peripheral wall portion provided on the entire circumference of the inner bottom surface portion, and a cutout portion in which a part of an upper end of the inner rising peripheral wall portion is cut out; A stacking step of welding the upper end of the internal rising peripheral wall portion and the lower surface of the inner bottom surface portion stacked on the upper end to obtain an internal multi-stage tray;
    Covering the upper end of the internal rising peripheral wall portion of the uppermost internal tray of the internal multi-stage tray with a lid, welding the upper end and the lid, and obtaining a covered internal multi-stage tray; The covered internal multi-stage tray is accommodated in an external housing including an external rising peripheral wall portion provided on the entire circumference of the external bottom surface portion and a port provided in a part of the external rising peripheral wall portion. And a housing step of welding the upper end of the external rising peripheral wall portion and the lid body to obtain an internal multi-stage tray housing case.
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