WO2014170460A3 - Method for the production of collagen proteins derived from marine sponges and an organism able to produce said proteins - Google Patents

Method for the production of collagen proteins derived from marine sponges and an organism able to produce said proteins Download PDF

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Publication number
WO2014170460A3
WO2014170460A3 PCT/EP2014/057966 EP2014057966W WO2014170460A3 WO 2014170460 A3 WO2014170460 A3 WO 2014170460A3 EP 2014057966 W EP2014057966 W EP 2014057966W WO 2014170460 A3 WO2014170460 A3 WO 2014170460A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
production
organism
produce
collagen proteins
Prior art date
Application number
PCT/EP2014/057966
Other languages
French (fr)
Other versions
WO2014170460A2 (en
Inventor
Marina POZZOLINI
Umberto Benatti
Francesca MUSSINO
Sonia Scarfi'
Valentina BERILLI
Annalisa Salis
Gianluca Damonte
Marco Giovine
Original Assignee
Universita' Degli Studi Di Genova
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universita' Degli Studi Di Genova filed Critical Universita' Degli Studi Di Genova
Publication of WO2014170460A2 publication Critical patent/WO2014170460A2/en
Publication of WO2014170460A3 publication Critical patent/WO2014170460A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Abstract

A method for the production of recombinant marine sponge collagen proteins, which method comprises introduction and expression of at least one nucleotide sequence coding for a primary polypeptide chain of marine sponge collagen proteins, and at least one nucleotide sequence coding for an enzymatic protein or at least one subunit thereof involved in the posttranslational modifications, such as hydroxylation, of said collagen proteins, in a host organism.
PCT/EP2014/057966 2013-04-18 2014-04-17 Method for the production of collagen proteins derived from marine sponges and an organism able to produce said proteins WO2014170460A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITGE2013A000040 2013-04-18
IT000040A ITGE20130040A1 (en) 2013-04-18 2013-04-18 METHOD FOR THE PRODUCTION OF RECOMBINANT MARINE COLLAGEN AND ORGANISM ABLE TO PRODUCE THE SEA COLLAGEN

Publications (2)

Publication Number Publication Date
WO2014170460A2 WO2014170460A2 (en) 2014-10-23
WO2014170460A3 true WO2014170460A3 (en) 2014-12-31

Family

ID=48628770

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2014/057966 WO2014170460A2 (en) 2013-04-18 2014-04-17 Method for the production of collagen proteins derived from marine sponges and an organism able to produce said proteins

Country Status (2)

Country Link
IT (1) ITGE20130040A1 (en)
WO (1) WO2014170460A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106119322B (en) 2016-07-22 2019-10-15 江苏江山聚源生物技术有限公司 A kind of zymotechnique improving recombination human source collagen production level
CA3008850A1 (en) * 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
RU2658428C9 (en) * 2017-10-03 2018-10-03 Общество с ограниченной ответственностью "Медсервис" Agent for treatment of human body states related to p4ha1 gene reduced expression and/or reduced quantity of prolyl 4-hydroxylase alpha 1 protein on basis of gene-therapeutic substances with p4ha1 gene, method of manufacture and operation
CN111334512B (en) * 2019-12-06 2023-10-13 肽源（广州）生物科技有限公司 Recombinant human-like collagen containing hydroxyproline and hydroxylysine and production method thereof
CN117126754A (en) * 2022-05-19 2023-11-28 华北制药集团新药研究开发有限责任公司 Recombinant type I collagen pichia pastoris engineering bacteria, construction method and application thereof
CN116333096B (en) * 2023-03-12 2023-10-24 百世美生物技术(浙江)有限公司 Application of recombinant human three-type collagen, injection and medical cosmetic product

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030032601A1 (en) * 2000-03-03 2003-02-13 Jorg Kreuter Method for isolating sponge collagen and producing nanoparticulate collagen, and the use thereof
EP1787995A1 (en) * 2005-11-17 2007-05-23 Technische Universität München Recombinant mussel byssus protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030032601A1 (en) * 2000-03-03 2003-02-13 Jorg Kreuter Method for isolating sponge collagen and producing nanoparticulate collagen, and the use thereof
EP1787995A1 (en) * 2005-11-17 2007-05-23 Technische Universität München Recombinant mussel byssus protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [Online] 6 August 2008 (2008-08-06), "Chondrosia reniformis nonfibrillar collagen mRNA, complete cds.", XP002715173, retrieved from EBI accession no. EM_STD:DQ874470 Database accession no. DQ874470 *
MARINA POZZOLINI ET AL: "Molecular Characterization of a Nonfibrillar Collagen from the Marine SpongeNardo 1847 and Positive Effects of Soluble Silicates on Its Expression", MARINE BIOTECHNOLOGY, SPRINGER-VERLAG, NE, vol. 14, no. 3, 10 November 2011 (2011-11-10), pages 281 - 293, XP035039502, ISSN: 1436-2236, DOI: 10.1007/S10126-011-9415-2 *
SWATSCHEK D ET AL: "Marine sponge collagen: isolation, characterization and effects on the skin parameters surface-pH, moisture and sebum", EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, ELSEVIER SCIENCE PUBLISHERS B.V., AMSTERDAM, NL, vol. 53, no. 1, 1 January 2002 (2002-01-01), pages 107 - 113, XP004331337, ISSN: 0939-6411, DOI: 10.1016/S0939-6411(01)00192-8 *

Also Published As

Publication number Publication date
ITGE20130040A1 (en) 2014-10-19
WO2014170460A2 (en) 2014-10-23

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