WO2014141066A3 - Polipéptido mutado, cepa de bacterias que lo comprende y métodos para detectar diferentes cationes metálicos simultáneamente - Google Patents

Polipéptido mutado, cepa de bacterias que lo comprende y métodos para detectar diferentes cationes metálicos simultáneamente Download PDF

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Publication number
WO2014141066A3
WO2014141066A3 PCT/IB2014/059629 IB2014059629W WO2014141066A3 WO 2014141066 A3 WO2014141066 A3 WO 2014141066A3 IB 2014059629 W IB2014059629 W IB 2014059629W WO 2014141066 A3 WO2014141066 A3 WO 2014141066A3
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WO
WIPO (PCT)
Prior art keywords
methods
metal cations
different metal
simultaneously detect
detect different
Prior art date
Application number
PCT/IB2014/059629
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English (en)
French (fr)
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WO2014141066A2 (es
Inventor
María Marta IBAÑEZ
Sebastián CERMINATI
Susana Karina CHECA
Fernando Carlos SONCINI
Original Assignee
Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet)
Universidad Nacional De Rosario
Inis Biotech Llc
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Publication date
Application filed by Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet), Universidad Nacional De Rosario, Inis Biotech Llc filed Critical Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet)
Publication of WO2014141066A2 publication Critical patent/WO2014141066A2/es
Publication of WO2014141066A3 publication Critical patent/WO2014141066A3/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Polipéptido mutado, cepas que lo comprende y métodos para detectar cationes metálicos, por ejemplo Au, Cu, Ag, Hg, Pb, Cd, Co y Zn, en muestras de manera simultánea, en donde el polipéptido comprende una sustitución de serina por cisteína en la posición 77 de la SEQ ID N° 1 o SEQ ID N° 2; y en donde dicha proteína tiene actividad de regulador transcripcional, por ejemplo GolS o CueR. El polipéptido detecta los metales como cationes metálicos monovalentes y divalentes.
PCT/IB2014/059629 2013-03-11 2014-03-11 Polipéptido mutado, cepa de bacterias que lo comprende y métodos para detectar diferentes cationes metálicos simultáneamente WO2014141066A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ARP20130100783 2013-03-11
ARP130100783A AR090303A1 (es) 2013-03-11 2013-03-11 Polipeptido mutado, cepa de bacterias que lo comprende y metodos para detectar diferentes cationes metalicos simultaneamente

Publications (2)

Publication Number Publication Date
WO2014141066A2 WO2014141066A2 (es) 2014-09-18
WO2014141066A3 true WO2014141066A3 (es) 2014-12-31

Family

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Family Applications (1)

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PCT/IB2014/059629 WO2014141066A2 (es) 2013-03-11 2014-03-11 Polipéptido mutado, cepa de bacterias que lo comprende y métodos para detectar diferentes cationes metálicos simultáneamente

Country Status (2)

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AR (1) AR090303A1 (es)
WO (1) WO2014141066A2 (es)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014597A1 (en) * 1997-09-15 1999-03-25 Vlaamse Instelling Voor Technologisch Onderzoek Metal ion specific capacity affinity sensor
WO2002030962A2 (en) * 2000-10-12 2002-04-18 University Of Georgia Research Foundation, Inc. Metal binding proteins, recombinant host cells and methods
WO2006069610A2 (en) * 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
WO2006110413A2 (en) * 2005-03-30 2006-10-19 Novartis Vaccines And Diagnostics Inc. Haemophilus influenzae type b
CN102250819A (zh) * 2010-05-18 2011-11-23 天津工业生物技术研究所 高灵敏检测重金属汞的生物传感器细胞及其制造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014597A1 (en) * 1997-09-15 1999-03-25 Vlaamse Instelling Voor Technologisch Onderzoek Metal ion specific capacity affinity sensor
WO2002030962A2 (en) * 2000-10-12 2002-04-18 University Of Georgia Research Foundation, Inc. Metal binding proteins, recombinant host cells and methods
WO2006069610A2 (en) * 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
WO2006110413A2 (en) * 2005-03-30 2006-10-19 Novartis Vaccines And Diagnostics Inc. Haemophilus influenzae type b
CN102250819A (zh) * 2010-05-18 2011-11-23 天津工业生物技术研究所 高灵敏检测重金属汞的生物传感器细胞及其制造方法

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BONTIDEAN I ET AL: "Bacterial metal-resistance proteins and their use in biosensors for the detection of bioavailable heavy metals", JOURNAL OF INORGANIC BIOCHEMISTRY, NEW YORK, NY, US, vol. 79, no. 1-4, 1 April 2000 (2000-04-01), pages 225 - 229, XP002224821, DOI: 10.1016/S0162-0134(99)00234-2 *
CHANGELA ANITA ET AL: "Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 301, no. 5638, 5 September 2003 (2003-09-05), pages 1383 - 1387, XP002431143, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1085950 *
DATABASE Geneseq [online] 10 December 2009 (2009-12-10), "Fumarate/malate production-related protein, SEQ:17753.", XP002729732, retrieved from EBI accession no. GSP:AXJ88351 Database accession no. AXJ88351 *
DATABASE Geneseq [online] 4 October 2007 (2007-10-04), "H. influenzae type B polypeptide HIB0292.", XP002729731, retrieved from EBI accession no. GSP:AEL79330 Database accession no. AEL79330 *
J. V. STOYANOV ET AL: "The Escherichia coli Copper-responsivecopA Promoter Is Activated by Gold", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 3, 22 November 2002 (2002-11-22), pages 1407 - 1410, XP055140116, ISSN: 0021-9258, DOI: 10.1074/jbc.C200580200 *
JAN ROELOF VAN DER MEER ET AL: "Where microbiology meets microengineering: design and applications of reporter bacteria", NATURE REVIEWS MICROBIOLOGY, vol. 8, no. 7, 1 June 2010 (2010-06-01), pages 511 - 522, XP055139833, ISSN: 1740-1526, DOI: 10.1038/nrmicro2392 *
SUSANA K CHECA ET AL: "Bacterial gold sensing and resistance", BIOMETALS, KLUWER ACADEMIC PUBLISHERS, BO, vol. 24, no. 3, 14 December 2010 (2010-12-14), pages 419 - 427, XP019900565, ISSN: 1572-8773, DOI: 10.1007/S10534-010-9393-2 *
ZHEN MA ET AL: "Coordination Chemistry of Bacterial Metal Transport and Sensing", CHEMICAL REVIEWS, vol. 109, no. 10, 29 September 2009 (2009-09-29), pages 4644 - 4681, XP055140062, ISSN: 0009-2665, DOI: 10.1021/cr900077w *

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Publication number Publication date
WO2014141066A2 (es) 2014-09-18
AR090303A1 (es) 2014-11-05

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