WO2014088948A1 - Dispositif pour électrophorèse en gel sans peigne - Google Patents

Dispositif pour électrophorèse en gel sans peigne Download PDF

Info

Publication number
WO2014088948A1
WO2014088948A1 PCT/US2013/072617 US2013072617W WO2014088948A1 WO 2014088948 A1 WO2014088948 A1 WO 2014088948A1 US 2013072617 W US2013072617 W US 2013072617W WO 2014088948 A1 WO2014088948 A1 WO 2014088948A1
Authority
WO
WIPO (PCT)
Prior art keywords
teeth
gel
plate
gel matrix
bottom edge
Prior art date
Application number
PCT/US2013/072617
Other languages
English (en)
Inventor
Zhuying Wang
Hong Qian
Yuanming Liao
Tao BAI
Original Assignee
Nanjingjinsirui Science & Technology Biology Corporation
Genscript Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjingjinsirui Science & Technology Biology Corporation, Genscript Corporation filed Critical Nanjingjinsirui Science & Technology Biology Corporation
Publication of WO2014088948A1 publication Critical patent/WO2014088948A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Definitions

  • the present invention relates to a device and methods for performing gel
  • the invention relates to an improved gel electrophoresis device for the separation of biological macromolecules that eliminates the need for a comb, allowing for easier handling and manufacture with improved gel resolution.
  • Gel electrophoresis is a commonly used technique for the separation of biological molecules such as proteins and nucleic acids (DNA and RNA).
  • the method involves applying an electric current to a porous, polymerized gel matrix that contains the biological mixture.
  • the components of the mixture will migrate through the gel matrix at different rates, most often dependent on charge and/or size. Movement of the molecules through the polymerized gel matrix produces a series of bands, with each band corresponding to a different molecule.
  • Acrylamide or agarose gel matrices are typically used for the separation of the biological molecules.
  • the gel matrix is formed by copolymerization with crosslinking reagents which creates a pore structure that allows for the passage of molecules through the gel matrix.
  • Other gels such as starch gels, have also been used for electrophoresis. Selection of the gel matrix material most often depends on the type of biological molecules to be separated. For protein separation, polyacrylamide gel electrophoresis (PAGE) is popular because
  • polyacrylamide gels are optically transparent, and the pore sizes are in a range that is suitable for proteins. Proteins with different charge/mass ratios will move through the polyacrylamide matrix at different rates. Using a series of known molecular weight proteins as a marker, the size and/or the molecular weight of the specific protein of interest can be estimated.
  • the basic apparatus used for gel electrophoresis includes (1) a gel cassette which holds the gel matrix between two plates and (2) an electrophoresis unit that holds the gel cassette and is connected to a power source which supplies the electric current that causes the molecules to move through the gel matrix.
  • the electrophoresis unit also contains buffer chambers that place the top and bottom of the gel in contact with the buffer solutions, which are ionic solutions that carry the electrical current through the gel matrix.
  • Glass or plastic plates have typically been used to form the gel cassettes for casting polyacrylamide gels. Polyacrylamide gels held in glass cassettes show better resolution compared to those held in plastic cassettes upon performing gel electrophoresis. However, glass is fragile and not suitable for high throughput production due to the tedious procedures taken to prepare the gels. Plastic molds are now used more often to make precast gels because it is easier and more economical. Special surface coating of the plastic plates has been adapted to improve the resolution of the separated macromolecule bands.
  • a standard gel cassette is formed by binding the two glass or plastic plates together and temporarily sealing the bottom of the cassette with tape. Spacers are placed along the vertical side edges of each plate to create a space, or "gel chamber,” between the plates for the gel matrix to fill.
  • the top edge of one plate is often cut away across the top length of the plate except at the vertical side edges to create a cutout that allows the buffer solution to access the top of the gel matrix once the cassette is placed in the electrophoresis unit.
  • the gel matrix solution is poured into the sealed cassette and then solidified through polymerization.
  • a comb is inserted between the two plates at the top edge prior to completion of the gel polymerization process with the teeth of the comb extending downwardly into the gel matrix.
  • the present invention provides an improved device for making a precast gel for performing gel electrophoresis, particularly for PAGE.
  • the device comprises two plastic plates to form a gel cassette with no comb needed to form sample wells.
  • the sample wells are formed by evenly spaced teeth configured on one or both of the two plates of the gel cassette without the need to insert and subsequently remove a separate comb.
  • One aspect of the present invention provides a gel cassette comprising a front plate and a back plate, wherein one of the two plates has at least two teeth configured on the inner surface of the plate near its top edge, such that at least one sample well is formed.
  • the spaces created between adjacent teeth at the top opening of the gel cassette define the sample wells of the gel chamber.
  • the thickness of the teeth is substantially equal to the spacing formed between the front and back plates.
  • either the front or back plate has 1 1 , 13 or 16 evenly spaced teeth configured on the plate to form a gel cassette with 10, 12 or 15 sample wells, respectively.
  • Another aspect of the present invention provides a gel cassette comprising a front plate and a back plate, wherein one of the two plates has at least two teeth configured on the inner surface of the plate near its top edge, and the other of the two plates has at least two vertical grooves configured on the inner surface of the plate near its top edge, such that the teeth align with the vertical grooves to form at least one sample well.
  • the thickness of the teeth is slightly greater than the spacing formed between the front and back plates, such that the teeth extend into the groove.
  • the spaces created between adjacent teeth extending into the corresponding grooves at the top opening of the gel cassette define the wells of the gel chamber used for sample loading.
  • the sample wells are bounded on opposite sides by the inner surfaces of the front and back plates, the side edges of adjacent teeth extending into the corresponding grooves and the top surface of the gel matrix.
  • one plate of the gel cassette has 11, 13, or 16 evenly spaced teeth, whereas the other plate has the same number of corresponding grooves, such that 10, 12, or 15 sample wells are formed respectively.
  • a gel cassette comprising a front plate and a back plate, wherein both plates have at least two teeth configured on their inner surfaces near their top edges.
  • the teeth are configured in such a way that two teeth (one from each plate) will pair with each other closely to form a pair of teeth, the pair acting as a wall of the sample wells when the front and back plates are fastened together.
  • at least two pairs of teeth will be formed, creating at least one sample well.
  • the thickness of the teeth is substantially equal to or slightly less than the spacing formed between the front and back plates.
  • each plate has 11, 13 or 16 evenly spaced teeth, such that 1 1, 13, or 16 pairs of teeth are formed that create 10, 12 or 15 sample wells respectively.
  • both the front and back plates have at least two teeth configured in their inner surfaces as described above, a gap is created between the two teeth of each pair that is preferably from 0.1 to 0.5 mm wide.
  • the gaps created between the two teeth of each pair will also be filled with the gel matrix due to capillary action, which will seal the wells and prevent sample leakage.
  • the invention also relates to methods of conducting gel electrophoresis comprising using a device according to an embodiment of the present invention.
  • the method comprises obtaining a gel cassette assembled according to an embodiment of the present invention, filling the gel chamber of the gel cassette with a gel matrix to a level just above the bottom edge of the teeth, and applying a sample to the sample wells.
  • FIG. 1 shows a side perspective view of a gel cassette comprising a front plate and a back plate with twelve sample loading wells assembled according to an embodiment of the present invention
  • FIG. 2 is a side plan view of a plastic plate according to an embodiment of the present invention.
  • FIG. 3 is a side perspective view of the inner surface of a back plate according to an embodiment of the present invention.
  • FIG. 4 is a side perspective view of the inner surface of a front plate according to an embodiment of the present invention DETAILED DESCRIPTION OF THE INVENTION
  • gel cassette refers to the device assembled from two plates aligned face-to-face and fastened together with a spacing formed between the two plates.
  • the spacing formed between the two plates is referred to as the "gel chamber.”
  • the gel matrix is held within the gel chamber.
  • top length of a plate refers to the dimension extending in a direction perpendicular to the vertical side edges of the plate along its top edge
  • plate-to- plate distance refers to the distance measured between the two plates extending in a direction perpendicular to the faces of the plates
  • top opening or “spacing at the top portion” refers to the space between the two plates accessible near the top edges of the plates
  • bottom opening or “spacing at the bottom portion” refers to the space between the two plates accessible near the bottom edges of the plates
  • gel thickness refers to the dimension of the gel in a direction perpendicular to the faces of the plates assembled to form the gel cassette.
  • well or “sample well” refer to the spaces created between adjacent teeth at the top opening of the gel cassette.
  • Well thickness refers to the dimension of the well in a direction perpendicular to the faces of the gel cassette plates.
  • tooth refers to the protrusions configured on the inner surface of the plate(s) near the top edge of the plate(s).
  • Each tooth has a front face that is substantially parallel to the inner surface of the plate and distal to the interface where the tooth is fused to the plate; two side edges, extending in a vertical direction, that are perpendicular to the inner surface of the plate from which the tooth extends; and a bottom edge, which is the surface of the tooth farthest from the top edge of the plate and perpendicular to the inner surface of the plate from which it extends.
  • teeth refers to the dimensions extending in a direction proximal to the top edge of the plate to the bottom edge of the tooth; “length” refers to the dimension in a direction extending across the front face of the tooth from one side edge to the other side edge, perpendicular to its height; and “thickness” refers to the dimension in a direction extending from the front face of the tooth to the interface where the tooth is fused to the inner surface of the plate.
  • standard gel cassette and “standard gel cassette and comb” all refer to a device for gel electrophoresis in which the plate-to-plate distance between the plates of the gel cassette is constant throughout. More specifically, the terms “standard 1 mm gel cassette” and “standard 1 mm gel cassette and comb” refer to a device for gel electrophoresis in which the plate-to-plate distance between the plates of the gel cassette, gel thickness and well thickness are constant throughout at 1 mm. The thickness of the teeth of the plates used with a standard 1 mm gel cassette is understood to be 1 mm.
  • FIG. 1 depicts a gel cassette, indicated by reference numeral 10, assembled according to embodiments of the present invention.
  • the gel cassette is comprised of a front plate 14 and back plate 12.
  • Each plate has an inner surface and an outer surface, with the inner surfaces oriented face-to-face with a space between the two plates. The space between the two plates defines the gel chamber and dictates the thickness of the gel matrix.
  • Each plate also has a top edge 20, a bottom edge 22, and two side edges 24, as shown in FIGS. 3 and 4, which respectively depict a side perspective view of the inner surfaces of a back plate and a front plate according to embodiments of the present invention.
  • At least two teeth are configured on the inner surface of at least one plate near its top edge, such that when the two plates are fastened together at least one sample well 18 (FIG. 1) is created without the need for inserting and subsequently removing a comb from the gel cassette.
  • the spaces created between adjacent teeth at the top opening of the gel cassette define the sample wells 18 of the gel chamber.
  • the gel matrix fills the gel chamber of the gel cassette to a level just above the bottom edge of the teeth, such that the gel matrix has a top surface that is just above the bottom edge of the teeth.
  • each sample well is bounded on opposite sides by the side edges of two adjacent teeth, the inner surfaces of the front and back plates, and the top surface of the gel matrix.
  • no comb is needed to be inserted into the gel matrix to form the wells, and no comb is needed to be pulled out prior to sample loading for performing gel electrophoresis. This will save the comb-related materials and labors.
  • the gel cassette is filled with a gel matrix to a level that is between 1 mm and 3 mm above the bottom edge of the teeth, such that the top surface of the gel matrix is between 1 mm and 3 mm above the bottom edge of the teeth.
  • the inner surfaces of the front and black plates are oriented face-to-face with their respective edges aligned during assembly of the gel cassette.
  • the two plates of the gel cassette can be assembled together via any suitable method such as ultrasonic welding. After assembly, the bottom opening of the cassette can or can not be sealed by any suitable tape, sealant, or polymeric materials.
  • the gel solution poured into the gel cassette can be any suitable matrix material, including but not limited to
  • the plates can be made of any suitable materials, preferably plastics including but not limited to polyethylene terephthalate, polyvinyl chloride, polymethyl methacrylate, acrylonitrile-styrene, polystyrene, polyethylene, or various copolymers.
  • the plates can also be transparent to facilitate the viewing of the electrophoresis process.
  • the front and back plates of the gel cassette have additional features such as bumps, cylinder-shaped posts 26, and corner shaped posts 28 (FIG. 3 and 4) at the side edges of the plates to facilitate assembly.
  • These additional features along the side edges of the plates also function as the spacers to space the plates apart and create a gel chamber in which the gel matrix is held.
  • the bumps function as spacers during the process of assembling the gel cassette, and the posts hold the two plates together and also avoid the deformation of the plates during the assembly process.
  • the front plate can also have an additional post 26 configured near its bottom edge to prevent the incurvation of the plates during assembly of the gel cassette (FIG. 4).
  • additional features can be made of any suitable materials, preferably plastics including but not limited to polyethylene terephthalate, polyvinyl chloride, polymethyl methacrylate, acrylonitrile-styrene, polystyrene, polyethylene, or various copolymers, and preferably are made of the same material as the plates. Any additional features for positioning and fastening the front and back plates together, such that they are spaced apart to create a gel chamber, can be used and are not limited to those embodiments specifically described here.
  • the inner surface of one plate of the gel cassette has at least two teeth configured near the top edge of the plate to form at least one rigid sample well when the front and the back plate are assembled together to form a gel cassette.
  • FIGS. 2 and 3 respectively show a side plan view and side perspective view of a back plate with teeth configured on its inner surface according to embodiments of the present invention.
  • the back plate has thirteen teeth 16, for creating twelve sample loading wells.
  • the back plate also has four cylinder-shaped posts 26 located at its side edges (FIG. 3), which are configured to fit into the corner-shaped posts 28 on the side edges of the front plate (FIG. 4) during assembly of the gel cassette to precisely position the plates together.
  • FIG. 3 the side edges
  • FIG. 4 the side edges of the front plate
  • the thickness of the teeth is substantially equal to the spacing formed between the front and back plates, such that the front face of each tooth is flush against the inner surface of the opposite plate. This essentially seals the sample wells and prevents sample leakage from the wells.
  • the spacing between the front and back plates is between 0.5 mm and 1.5 mm, such that the teeth have a thickness ti (FIG. 1) also between 0.5 mm to 1.5 mm.
  • the teeth also preferably have a length li ranging from 0.5 to 3 mm, and a height i ranging from 5 mm to 15 mm (FIG. 2).
  • the front plate has at least two teeth configured on its inner surface near its top edge for forming at least one sample well when assembled with a back plate to form a gel cassette.
  • FIG. 4 depicts a side perspective view of a front plate 14 according to an embodiment of the present invention.
  • the front plate has thirteen teeth for creating twelve sample loading wells.
  • the front plate also has four corner- shaped posts 28 located at its side edges configured to fit into the cylinder-shaped posts 26 on the side edges of the back plate during assembly of the gel cassette to precisely position the plates together (FIG. 3), as discussed above.
  • the top edge of the front plate can also contain a cutout across its top length, except at the two side edges, to allow access of the top surface of the gel matrix to the buffer solution during electrophoresis and to facilitate sample loading.
  • the top edge of the front plate can be lower than the top edge of the back plate in the assembled gel cassette.
  • either the front plate or the back plate has 11 , 13, or 16 evenly spaced teeth configured on its inner surface near its top edge, such that 10, 12, or 15 sample wells are created after assembly with the other plate to form the gel cassette.
  • one plate of the gel cassette (either the front or back plate) has at least two teeth configured on its inner surface near its top edge
  • the other plate of the gel cassette has at least two vertical tooth grooves configured on its inner surface near its top edge.
  • the terms "groove” and “grooves” also refer to the vertical tooth grooves described above.
  • the teeth on one plate and the corresponding tooth grooves on the other plate are configured such that the teeth align with the grooves and fit into the grooves when the plates are assembled together to form the gel cassette.
  • the purpose of the grooves is to hold the teeth tightly in place and create a seal that prevents sample leakage to other wells.
  • the grooves are at least 0.55 mm long, 0.15 mm wide and 0.05 mm deep.
  • the thickness of the teeth is slightly larger than the spacing formed between the front and back plates, such that the teeth extend into the corresponding tooth grooves.
  • the spacing between the front and back plates is preferably between 0.5 and 1.5 mm, such that the teeth have a thickness between 0.5 mm to 2 mm, as well as a length li ranging from 1 mm to 3 mm and a height hi ranging from 5 mm to 15 mm.
  • the number of teeth configured on one plate is equivalent to the number of tooth grooves configured on the other plate of the gel cassette.
  • one plate has 11, 13 or 16 evenly spaced teeth and the other plate also has 11, 13, or 16 evenly spaced corresponding tooth grooves, such that 10, 12, or 15 sample wells are created respectively, after the two plates are assembled together to form the gel cassette.
  • both the front and back plates have at least two teeth configured on their inner surfaces near their top edges.
  • the teeth are configured in such a way that when the two plates are assembled together, two teeth (one from each plate) will stand closely side-by-side with the side edges aligned parallel to one another to form a pair of teeth.
  • At least two teeth are on each plate, such that at least two pairs of teeth are formed to create at least one sample well. After the gel matrix is filled to a level just above the bottom edge of the teeth, each sample well is bounded on opposite sides by the inner surfaces of the front and back plates, two adjacent pairs of teeth, and the top surface of the gel matrix.
  • the thickness of the teeth is substantially equal to or slightly less than the spacing formed between the front and back plates.
  • the teeth have a length U ranging from 1 mm to 3 mm, a thickness t! ranging from 0.5 mm to 1.5 mm, and a height h ⁇ ranging from 5 mm to 15 mm.
  • a gap is formed between the two teeth of each pair.
  • the gap defining the tooth-to-tooth distance from the side edge of one tooth of the pair to the side edge of the other tooth of the pair, preferably has a width ranging from 0.1 mm to 0.5 mm.
  • both the front plate and back plates each have 11, 13 or 16 evenly spaced teeth configured on their inner surfaces near their top edges.
  • 11, 13 or 16 evenly spaced pairs of teeth are formed, creating 10, 12, or 15 corresponding sample wells in the gel cassette, respectively.
  • the present invention also relates to a method of using a device according to an embodiment of the present invention for electrophoresis.
  • the device can be used for any type of electrophoresis using methods known in the art in view of the present disclosure.
  • Various factors such as the dimensions of the device or gel matrix, the type of gel casted in the device, the number of teeth, the electrophoresis buffer solution, etc. can be adjusted depending on the need.
  • a method for performing gel electrophoresis comprises obtaining a gel cassette assembled according to an embodiment of the present invention; filling the gel chamber of the gel cassette with a gel matrix to a level just above the bottom edge of the teeth such that the at least one sample well is bounded on opposite sides by the inner surfaces of the front and back plates, the teeth, and the top surface of the gel matrix; and applying a sample to the sample well.
  • the method may comprise additional standard steps of placing the gel cassette in an electrophoresis unit, filling the unit with an electrophoresis buffer, and applying a current to the gel matrix, and these additional steps will be well known to one skilled in the art.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention porte sur un dispositif pour électrophorèse en gel ayant de multiples puits de chargement d'échantillon formés sans l'aide de quelconques peignes. Le dispositif comprend une cassette de gel ayant une plaque avant et une plaque arrière, l'une des plaques ayant des dents espacées de manière régulière configurées sur sa surface intérieure près de son bord supérieur pour créer des puits de chargement d'échantillon rigides au niveau de l'ouverture supérieure de la cassette de gel, et une matrice de gel. A l'aide du dispositif de la présente invention, aucun peigne n'est nécessaire durant une formation de matrice de gel et ainsi, les gels sont prêts pour le chargement d'échantillon sans retrait de quelconques peignes, économisant les matières et travaux associés à un peigne. La présente invention porte également sur des procédés d'utilisation du dispositif pour réalisation d'électrophorèse en gel.
PCT/US2013/072617 2012-12-03 2013-12-02 Dispositif pour électrophorèse en gel sans peigne WO2014088948A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261732559P 2012-12-03 2012-12-03
US61/732,559 2012-12-03

Publications (1)

Publication Number Publication Date
WO2014088948A1 true WO2014088948A1 (fr) 2014-06-12

Family

ID=49841824

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/072617 WO2014088948A1 (fr) 2012-12-03 2013-12-02 Dispositif pour électrophorèse en gel sans peigne

Country Status (1)

Country Link
WO (1) WO2014088948A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020070115A1 (en) * 2000-10-26 2002-06-13 Shaun Atchison Cassette assembly for electrophoresis gels
US6582577B1 (en) * 2000-08-31 2003-06-24 Visible Genetics Inc. Electrophoresis gel cassette
US20110084194A1 (en) * 2009-09-24 2011-04-14 Dgel Sciences Cassette for biological analysis and method of making thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582577B1 (en) * 2000-08-31 2003-06-24 Visible Genetics Inc. Electrophoresis gel cassette
US20020070115A1 (en) * 2000-10-26 2002-06-13 Shaun Atchison Cassette assembly for electrophoresis gels
US20110084194A1 (en) * 2009-09-24 2011-04-14 Dgel Sciences Cassette for biological analysis and method of making thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DAVIS: "Disc Electrophoresis 2, Method and application to human serum proteins", ANN. NEW YORK ACAD SCI, vol. 121, 1964, pages 404 - 427
ORNSTEM, DISC ELECTROPHORESIS 1, BACKGROUND AND THEORY ANN NEW YORK ACAD SCI, vol. 121, 1964, pages 321 - 349
RAYMOND; WEMTRAUB, ACRYLAMIDE GEL AS A SUPPORTING MEDIUM FOR ZONE ELECTROPHORESIS SCIENCE, vol. 130, 1959, pages 711 - 711

Similar Documents

Publication Publication Date Title
US20200278317A1 (en) Electrophoresis gel cassette and comb
US5284565A (en) Sample well insert with wedge-shaped profile for ultra-thin slab gels in electrophoresis
US4385974A (en) Electrophoretic system and method for multidimensional analysis
EP2923198B1 (fr) Dispositif d'électrophorèse sur gel pour le chargement de grands volumes d'échantillon
US5989403A (en) Electrophoresis assembly with filling means
US7135101B2 (en) Cassette assembly for electrophoresis gels
EP2986978B1 (fr) Peigne pour mini-gel
US20150160156A1 (en) Cassette for electrophoresis and electrophoresis method
US6655649B2 (en) Assembly for casting and use of an isoelectric focusing strip
WO2003048754A1 (fr) Dispositif a gel d'electrophorese et son procede d'assemblage
JP2005530151A (ja) プレキャスト水和可能分離媒体の低抵抗電気泳動のための方法および装置
WO2014088948A1 (fr) Dispositif pour électrophorèse en gel sans peigne
KR102645006B1 (ko) 전기 영동용 아가로스 겔 제조보관틀
AU2002210242B2 (en) Cassette assembly for electrophoresis gels
WO2002042758A1 (fr) Procede et dispositif de formation de puits dans des plaques de gel d'electrophorese
AU2002210242A1 (en) Cassette assembly for electrophoresis gels

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13811306

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13811306

Country of ref document: EP

Kind code of ref document: A1