WO2013186644A2 - Système de détection et de suivi de placement d'une substance dans un réceptacle d'échantillon - Google Patents

Système de détection et de suivi de placement d'une substance dans un réceptacle d'échantillon Download PDF

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Publication number
WO2013186644A2
WO2013186644A2 PCT/IB2013/054122 IB2013054122W WO2013186644A2 WO 2013186644 A2 WO2013186644 A2 WO 2013186644A2 IB 2013054122 W IB2013054122 W IB 2013054122W WO 2013186644 A2 WO2013186644 A2 WO 2013186644A2
Authority
WO
WIPO (PCT)
Prior art keywords
micro
plate
tracking device
detecting device
placing
Prior art date
Application number
PCT/IB2013/054122
Other languages
English (en)
Other versions
WO2013186644A3 (fr
Inventor
Matjaž HREN
Klemen ZUPANČIČ
Urška ČEPIN
Matjaž MIHELJ
Aleš HRIBAR
Original Assignee
Biosistemika D.O.O.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosistemika D.O.O. filed Critical Biosistemika D.O.O.
Publication of WO2013186644A2 publication Critical patent/WO2013186644A2/fr
Publication of WO2013186644A3 publication Critical patent/WO2013186644A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Definitions

  • the present invention refers to a system for vertically adjusting a deposit container in a door of a household appliance, particularly cooling and/or freezing apparatus, comprising a body with a compartment for accepting goods to be cooled, a door for opening and closing said compartment with at least one deposit container, said door comprising on the inner wall thereof at least one fastening rib for fixing said deposit container.
  • a deposit container on a door of a household appliance is intended for depositing and/or storing food, bottles and similar, which may in turn comprise different heights.
  • several solutions are available enabling the adjustment of the deposit container to a new position.
  • a patent application No. EP 2 108 907 Al discloses a solution comprising a mechanism with several parts and a plastic spring which enables relocation of a pawl on a wave-shaped section and, thus, a vertical adjustment of the deposit container. Said solution is relatively complex and makes cleaning of the parts rather difficult.
  • the object of the present invention is to create a system for vertically adjusting a deposit container in a door of a household appliance, particularly cooling and/or freezing apparatus which remedies drawbacks of know solutions.
  • each fastening rib which is arranged on a rail shaped projection formed in each vertical marginal area in an inner wall of said door, cooperates with a deposit container and at least one snap-action means inserted into a correspondent receptacle of said container.
  • each transversal confine of said container is formed over the entire height thereof, in the area of the association with a back wall of the deposit container, with a vertical groove fitted to receive and to cooperate by means of a loose fit with each said fastening rib.
  • Each said groove is provided in the area of the transition to said transversal confine with a hollow box-shaped receptacle, each of said receptacles being formed at the side of each transversal confine with a through-hole into which snaps-in a transversal projection of the snap-action means.
  • Fig. 1 shows a three-dimensional exploded view of a system for vertically adjusting a deposit container in a door of a household appliance
  • Fig. 2 shows a three-dimensional view of a snap-action means of a system of Fig. 1
  • Fig. 3 shows a three-dimensional view of a deposit container of a system of Fig. 1;
  • Fig. 4 shows a sectional view of a system of Fig. 1 through a snap-action means in a vertical plane.
  • a system for vertically adjusting a deposit container in a door of a household appliance, particularly cooling and/or freezing apparatus is formed on the side of a door 1 facing the interior of a household appliance not shown.
  • Said door 1 comprises at the side facing the interior of cooling and/or freezing apparatus an inner wall 2 which is formed in each vertical marginal area 3, 4 thereof with a rail shaped projection 5, 6 extending, essentially, over the entire height of the door 1.
  • Each said projection 5, 6 comprises at the side facing the opposite projection 5, 6 a fastening rib 7, 8 in order to vertically fix a deposit container 9.
  • each fastening rib 7, 8 is arranged on each projection 5, 6 spaced from the inner wall, in the present preferred embodiment it is arranged in the central area between the association of each projection 5, 6 with the inner wall 2 and the free end of each projection 5, 6.
  • said inner wall is made of plastic material with each said fastening rib 7, 8 being formed by means of a blow moulding.
  • each fastening rib 7, 8 is formed in a similar manner as said fastening rib 7, 8 an end stop 10, 11; 10', 11' preventing positioning the deposit container 9 to close to the lower edge of the door 1. It is provided for according to the present invention that each fastening rib 7, 8 is formed essentially over the entire length thereof with fastening means 12, 13.
  • said fastening means 12, 13 are formed on each fastening rib 7, 8 at the side facing away from said inner wall 2 of the door 1. Further, an embodiment is possible where said fastening means 12, 13 are formed on each fastening rib 7, 8 at the facing side each other. Furthermore, with the present embodiment said fastening means 12, 13 are formed in a manner of a rack with a mutually equidistant spaced peaks and troughs. Obviously, alternative embodiments of the rack are possible such as the fastening ribs 7, 8 with recesses, raises and similar spaced more or less equidistantly in height.
  • said fastening rib 7, 8 is preferably formed integrally with said inner wall 2 of said door 1, preferably by means of a blow moulding. Additionally, each said fastening rib 7, 8 is provided at the top free end thereof with a convenient chamfer which facilitates accommodation of the deposit container 9 on the fastening ribs 7, 8.
  • said deposit container 9 is formed specular symmetrically with respect to the vertical, and accommodated on said door 1 in a longitudinal area between said rail- shaped projections 5, 6 and fastening ribs 7, 8, respectively.
  • each transversal confine 14, 15 of said container 9 is formed over the entire height thereof and in the area of the association with a back wall 16, i.e. in the area located close to said wall 2 of the door 1, with a vertical groove 17, 18 the transversal dimension thereof being fitted to accept and to cooperate by means of a loose fit with each fastening rib 7, 8.
  • said groove 17, 18 can be formed in a manner to extend in the transversal direction of said container 9 up to said back wall 16, thus, creating a step 19, 20 between said back wall 16 and each said transversal confine 14, 15.
  • Said groove 17, 18 is provided in the transitional area to said transversal confine 14, 15 with a hollow box-shaped receptacle 21, 22, each one thereof being formed at the side of each transversal confine 14, 15 with a through-hole 23, 24.
  • Each receptacle 21, 22 is provided for the accommodation of a snap-action means 25, 26 cooperating with the fastening means 12, 13 of each fastening rib 7, 8.
  • said deposit container 9 is provided with only one snap-action means.
  • said snap-action means 25, 26 are of identical shape, an embodiment is possible, though, with said snap-action means 25, 26 shaped differently with regard to the position thereof in the deposit container 9, where in such a case the second snap-action means is shaped specular symmetrically with respect to the first snap-action means in a manner as described above for said deposit container 9. Therefore, only the first snap- action means 25 is described in the foregoing.
  • Said first snap-action means 25 is formed as a two-legged fastener with a first flat leg 27 thereof being longer than a second flat leg 28.
  • the transversal dimension of said two- legged fastener is such that said fastener cooperates by means of a loose fit with said receptacle 21, 22 of the deposit container 9.
  • said flat legs 27, 28 extend in parallel and are associated by means of a web 29 which enables a flexible movement of the legs 27, 28 against each other.
  • Said second leg 28 is formed from the free end thereof up to the area of said web 29 with a longitudinally extending cut 30 which allows the portions of the second leg 28 created by said cut to be moved back and forth relative to each other.
  • said second leg 28 is formed in the area of the free end thereof and at least at one side of said free end with a transversally extending projection 31 intended to cooperate with the through-hole 23, 24 of said container 9.
  • the free end of said projection 31 is preferably formed with a slanted surface 31' the inclination thereof decreases in the direction from said free end of the second leg 28 towards said web 29.
  • Said first leg 27 is formed at the free end thereof with a transversal enlargement 32 intended to facilitate the cooperation with a user.
  • Said first leg is formed in the area between said web 29 and said transversal enlargement 32 with a convexity 33 which is intended to engage the trough of the rack formed fastening means 12, 13 when the snap-action means 25 is installed.
  • Said convexity 33 resembles an approximate V shape and extends approximately in the direction perpendicular to the surface-wide portion of said flat first leg 27. According to the present invention it is provided for that both the convexity 33 of each snap-action means 25, 26 and the corresponding troughs of each fastening means 12, 13 are formed with an undercut, thus, enabling a safe accommodation of the deposit container 9 on said fastening means 12, 13. Said deposit container 9 is placed on to the inner side of said door 1 of a cooling and/or freezing apparatus in the following way. Said snap-action means 25, 26 is inserted into at least one said receptacle 21, 22 of the deposit container 9, preferably into both receptacles 21, 22.
  • the assembly container 9 - snap-action means 25, 26 is placed on the door 1 in a manner that each vertical groove 17, 18 of the container 9 locks by means of the loose fit to the corresponding fastening rib, whereas said convexity 33 of the snap-action means 25, 26 engages the trough of the rack formed fastening means 12, 13.
  • said convexity 33 disengages said trough of the rack formed fastening means 12, 13 by means of pushing each transversal enlargement 32 of each first leg 27 of the two-legged fastener in the direction away from said wall of the door 1.
  • the deposit container 9 is released and ready to be vertically adjusted in the door 1.
  • the present invention refers to a system to detect and track placing of a substance into a sample receptacle, particularly to detect and track placing of the extremely small fluid volumes into micro-plates, preferably when performing laboratory analysis.
  • PCR real-time polymerase chain reaction
  • ELISA enzyme linked immunosorbent assay
  • Said plates are a disposable laboratory material, usually made of transparent plastic.
  • the basic problem related to said plates is the large number of very small wells contained therein, where qPRC reaction takes place, for instance.
  • the dimensions of said wells depend on the size of the plates and the number of wells in the plate, respectively, and typically amount to 3,1 mm, for instance, with a mutual spacing of less than 4,5 mm between neighbouring wells.
  • Liquid substances are introduced into said wells according to predetermined plan, which may be a very complex one, leading consequently to errors, since a user has to be absolutely concentrated in order to complete the complex job. Tracking with eyes which well in the plate has already been filled with a substance is rather impractical and unreliable. It is the object of the present invention to create a system to detect and track placing of a substance into a sample receptacle, which will remedy the drawbacks of the known solutions.
  • Fig. 1 shows a schematic view of a system to detect and track placing of a sample in a sample receptacle according to the invention
  • Fig. 2 shows another embodiment of a system of Fig. 1.
  • a first step in a system for detecting and tracking according to the invention is creating a plan for entering fluid samples into wells of a micro-plate 1.
  • Said plan is prepared in a processor unit 2, either locally or remotely, for instance, and transferred to a tracking device 4 by means of a data line 3 such as for example a wired line, a wire-less line, a memory key, and similar.
  • a data line 3 such as for example a wired line, a wire-less line, a memory key, and similar.
  • a user can save and share such plans with other users.
  • said plan is a digitized form of the plan of the micro-plate 1, where the order of the sample input is the major information comprised in said plan.
  • Said tracking device 4 which simultaneously serves as a source of a transmitted light when entering fluid samples in wells of the micro-plate 1, has been selected as a flat and thin screen, such as for instance an LCD screen, an AMOLED screen, a LED diodes matrix and similar, in the presented embodiment as a touch sensitive LCD screen having a processing unit.
  • said micro-plate 1 is placed over said tracking device 4 and additionally provided with a detecting device 5 for detection of the fluid input, whereupon said plan for entering the fluid samples is triggered, said plan being stored in said tracking device 4.
  • a detecting device 5 for detection of the fluid input
  • said plan for entering the fluid samples is triggered, said plan being stored in said tracking device 4.
  • light sources that illuminate specific wells on micro-plate 1 into which a fluid sample is to be entered are activated on the screen of said tracking device 4 arranged under said micro-plate 1.
  • said detecting device 5 Based on the interference, for instance, triggered in the device 5 by means of a pipette tip when entering a fluid sample into the micro-plate 1, said detecting device 5 transmits information to the tracking device 4 via data line 3', either wired or wire-less.
  • said detecting device 5 for detection the fluid entering is arranged above or below said micro-plate 1, thus, enabling different possibilities of entering fluid samples into wells of said micro-plate 1.
  • the spacing of said detecting device 5 from the micro- plate 1 is relatively small.
  • One of the possible embodiments of the arrangement of said detecting device 5 above the micro-plate 1 provides for the use of a light field 6 created by
  • INCORPORATED BY REFERENCE (RULE 20.6) 3 means of at least one light source 7 and at least one light detector 8.
  • said light field 6 is created between two light sources 7 placed perpendicularly to each other and two light detectors 8 placed opposite thereto, said light detectors 8 detecting the light emitted by said light sources 7.
  • Said light field 6 may be created by means of different light origins such as at least one laser, diodes, combination of at least one camera with the surrounding light and similar.
  • one of possible embodiments of the arrangement of said detecting device 5 below the micro-plate 1 provides for the use of a force originating at the contact of the device for entering sample, such as the pipette tip, with the micro-plate 1.
  • at least one sensor 8 preferably a plurality of sensors, such as accelerometers, dynamo-meters and similar that meter said small forces is arranged directly under the micro-plate 1 or under said tracking device 4.
  • the detecting device 5 is arranged above said micro-plate, a light field 6 being used as a detecting device 5.
  • the first light source is activated on said tracking device 4 which illuminates the first well of the micro-plate 1 into which the fluid sample is to be introduced.
  • the detecting device 5 is triggered by means of the tip of the pipette, in the present embodiment said light field 6 is penetrated at a given point of the coordinate system formed by said light field 6.
  • This disturbance in the light field is communicated via said data line 3' to the tracking device 4 which, in turn, by means of an appropriate algorithm not being subject of the present invention, senses the penetration of said light field 6 and/or the location of said penetration of said light field 6, thus unambiguously determining coordinate of the well of the micro-plate 1 into which the fluid sample has been deposited. Furthermore, said tracking device 4 simultaneously tests if said sensed coordinate match with a coordinate stored in said plan. If said sensed coordinate matches the coordinate stored in said plan, the system according to the invention switches over to the next step where said light source illuminates the next well being in turn for the fluid to be introduced according to said stored plan. In the case of false coordinates of the fluid deposition into micro-plate, the system alerts the user of the erroneous selection. In addition to said activation of light sources, additional data are shown at the upper part of the screen, such as, for instance, the name of the next coordinate of the well to be filled with a sample, description of the sample to be deposited, and similar.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

La présente invention concerne un système pour détecter et suivre une entrée d'échantillon dans un réceptacle d'échantillon, en particulier pour détecter et suivre l'entrée de volumes de fluide extrêmement petits dans des microplaques, de préférence lors d'une réalisation d'analyse laboratoire. Le système selon l'invention comprend une unité de traitement (2), fixée au moyen d'une ligne de données (3) à un dispositif de suivi (4) placé dans la zone sous une microplaque (1), et un dispositif de détection (5) pour détecter l'entrée de fluide.
PCT/IB2013/054122 2012-05-24 2013-05-20 Système de détection et de suivi de placement d'une substance dans un réceptacle d'échantillon WO2013186644A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SI201200160A SI24077A (sl) 2012-05-24 2012-05-24 Sistem za detekcijo in sledenje vnosa snovi v sprejemnik vzorcev
SIP-201200160 2012-05-24

Publications (2)

Publication Number Publication Date
WO2013186644A2 true WO2013186644A2 (fr) 2013-12-19
WO2013186644A3 WO2013186644A3 (fr) 2014-03-06

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PCT/IB2013/054122 WO2013186644A2 (fr) 2012-05-24 2013-05-20 Système de détection et de suivi de placement d'une substance dans un réceptacle d'échantillon

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SI (1) SI24077A (fr)
WO (1) WO2013186644A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2843333A1 (fr) * 2014-07-23 2015-03-04 Gorenje d.d. Système amélioré pour déplacer verticalement un balconnet de porte dans la porte d'un appareil ménager
EP3064875A1 (fr) * 2015-03-06 2016-09-07 Gorenje Gospodinjski aparati d.d. Appareil ménager comportant un récipient de dépôt réglable en hauteur d'une porte

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2108907A1 (fr) 2008-04-09 2009-10-14 BSH Bosch und Siemens Hausgeräte GmbH Appareil de refroidissement, notamment appareil de refroidissement ménager doté d'un rail de porte en plusieurs parties pour un balconnet

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7544330B2 (en) * 2005-09-28 2009-06-09 Idexx Laboratories, Inc. Microplate sample tracking system
US20080031774A1 (en) * 2006-03-13 2008-02-07 Sage Science, Inc. Apparatus for Guiding Sample and Reagent Manipulations and Receptacles for Holding Same
US8580197B2 (en) * 2008-04-24 2013-11-12 Tecan Trading Ag Direct pipetting in computer-controlled liquid handling workstations
CH702942A1 (de) * 2010-03-31 2011-10-14 Tecan Trading Ag Kapazitives Messverfahren und Vorrichtung zur Füllstandsdetektion und entsprechend ausgestattetes Laborgerät.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2108907A1 (fr) 2008-04-09 2009-10-14 BSH Bosch und Siemens Hausgeräte GmbH Appareil de refroidissement, notamment appareil de refroidissement ménager doté d'un rail de porte en plusieurs parties pour un balconnet

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2843333A1 (fr) * 2014-07-23 2015-03-04 Gorenje d.d. Système amélioré pour déplacer verticalement un balconnet de porte dans la porte d'un appareil ménager
EP3064875A1 (fr) * 2015-03-06 2016-09-07 Gorenje Gospodinjski aparati d.d. Appareil ménager comportant un récipient de dépôt réglable en hauteur d'une porte

Also Published As

Publication number Publication date
WO2013186644A3 (fr) 2014-03-06
SI24077A (sl) 2013-11-29

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