WO2013050867A3 - A chitinase from brevibacillus laterosporus, its production and use thereof - Google Patents

A chitinase from brevibacillus laterosporus, its production and use thereof Download PDF

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Publication number
WO2013050867A3
WO2013050867A3 PCT/IB2012/001982 IB2012001982W WO2013050867A3 WO 2013050867 A3 WO2013050867 A3 WO 2013050867A3 IB 2012001982 W IB2012001982 W IB 2012001982W WO 2013050867 A3 WO2013050867 A3 WO 2013050867A3
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WO
WIPO (PCT)
Prior art keywords
chitinase
new
insecticidal
lak
agriculture
Prior art date
Application number
PCT/IB2012/001982
Other languages
French (fr)
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WO2013050867A4 (en
WO2013050867A2 (en
Inventor
Lakshmi Prasanna GANGAVARAMU
Original Assignee
Gangavaramu Lakshmi Prasanna
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Publication date
Application filed by Gangavaramu Lakshmi Prasanna filed Critical Gangavaramu Lakshmi Prasanna
Publication of WO2013050867A2 publication Critical patent/WO2013050867A2/en
Publication of WO2013050867A3 publication Critical patent/WO2013050867A3/en
Publication of WO2013050867A4 publication Critical patent/WO2013050867A4/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a new strain of Brevibacillus later osporus LAK 1210 (MTCC 5487) having an accession number MTCC 5487, as a dual producer of insecticidal proteins and inducible chitinolytic enzymes with a potential utility in agriculture and forest insect pest management, plant disease control and mosquito control programs. The multiple effects of this new insecticidal strain of Brevibacillus laterosporus LAK 1210, as a chitinase producer with the ability to degrade marine wastes finds many biotechnological applications in the areas of agriculture, forestry, medicine and environment. Furthermore, the object of the invention is also directed to a new chitinase with novel characteristics from a newer source of microorganism and its novel applications in biomass utilization and waste management. Methods and compositions of the invention utilize the vegetative cells, their derivatives (spores, cellular extracts, enzyme fractions) and the polypeptides to develop a natural pesticide for control of insects and phytopathogenic fungi. Another object of this invention is to expand the synergistic effect of chitinases on insecticidal activity for an improved pesticidal activity where a wider range of pests are impacted.
PCT/IB2012/001982 2011-10-08 2012-10-08 A chitinase from brevibacillus laterosporus, its production and use thereof WO2013050867A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161545120P 2011-10-08 2011-10-08
US61/545,120 2011-10-08

Publications (3)

Publication Number Publication Date
WO2013050867A2 WO2013050867A2 (en) 2013-04-11
WO2013050867A3 true WO2013050867A3 (en) 2013-10-24
WO2013050867A4 WO2013050867A4 (en) 2013-12-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2012/001982 WO2013050867A2 (en) 2011-10-08 2012-10-08 A chitinase from brevibacillus laterosporus, its production and use thereof

Country Status (1)

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WO (1) WO2013050867A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10004236B2 (en) * 2012-09-24 2018-06-26 Lincoln University Biocontrol compositions
EP3038469A4 (en) * 2013-08-12 2017-06-21 Bio-Cat Microbials LLC Compositions comprising bacillus strains and methods of use to suppress the activities and growth of fungal plant pathogens
CN104480046B (en) * 2014-12-18 2017-04-05 江苏省农业科学院 One plant of Brevibacillus laterosporus and its application
CA3168036A1 (en) 2017-03-27 2018-10-04 Tenfold Technologies, LLC Methods and agricultural compositions for preventing or controlling plant diseases
CN107151641B (en) * 2017-07-04 2020-11-27 沈阳农业大学 Brevibacillus laterosporus for inhibiting rhizoctonia solani and application thereof
US11168314B2 (en) 2017-09-05 2021-11-09 Department Of Biotechnology Modified chitinase from Brevibacillus laterosporus LAK 1210 and uses thereof
CN110699276A (en) * 2019-09-30 2020-01-17 广西民族大学 Strain of chitin-like paenibacillus and application thereof
CN112779178B (en) * 2019-11-08 2023-02-17 财团法人农业科技研究院 Bacillus licheniformis A6 strain and application thereof
CN111454848B (en) * 2020-04-15 2022-04-19 中国科学院微生物研究所 Actinomucor elegans GD48, fermentation product, microbial inoculum and application thereof
CN114480197B (en) * 2022-02-09 2022-10-04 山东农业大学 Brevibacillus laterosporus, microbial inoculum and application thereof in biological pesticide fertilizer
CN114456987B (en) * 2022-03-11 2023-11-24 天津科技大学 Preparation method and application of Brevibacillus laterosporus, spore antibacterial peptide-chitosan-gelatin composite preservative film
CN115364123B (en) * 2022-07-05 2023-08-25 江苏三仪生物工程有限公司 Brevibacillus laterosporus and application thereof in ruminant parasite control

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5045314A (en) * 1989-11-14 1991-09-03 The United States Of America As Represented By The Secretary Of Agriculture Control of parasitic nematode ova/larvae with a bacillus laterosporus
WO2008031887A2 (en) * 2006-09-15 2008-03-20 Universita' Degli Studi Di Sassari Brevibacillus laterosporus strain compositions containing the same and method for the biological control of dipters

Family Cites Families (2)

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Publication number Priority date Publication date Assignee Title
US3911110A (en) 1972-12-22 1975-10-07 Canadian Patents Dev Insecticidal compositions
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5045314A (en) * 1989-11-14 1991-09-03 The United States Of America As Represented By The Secretary Of Agriculture Control of parasitic nematode ova/larvae with a bacillus laterosporus
WO2008031887A2 (en) * 2006-09-15 2008-03-20 Universita' Degli Studi Di Sassari Brevibacillus laterosporus strain compositions containing the same and method for the biological control of dipters

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DE OLIVEIRA E J ET AL: "Molecular characterization of Brevibacillus laterosporus and its potential use in biological control", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 11, 1 November 2004 (2004-11-01), pages 6657 - 6664, XP002571305, ISSN: 0099-2240, DOI: 10.1128/AEM.70.11.6657-6664.2004 *
LAKSHMI PRASANNA ET AL: "A novel strain of Brevibacillus laterosporus produces chitinases that contribute to its biocontrol potential", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 97, no. 4, 29 April 2012 (2012-04-29), pages 1601 - 1611, XP055069226, ISSN: 0175-7598, DOI: 10.1007/s00253-012-4019-y *
M. LIU ET AL: "Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity", JOURNAL OF APPLIED MICROBIOLOGY, vol. 93, no. 3, 1 September 2002 (2002-09-01), pages 374 - 379, XP055069213, ISSN: 1364-5072, DOI: 10.1046/j.1365-2672.2002.01693.x *
SAIKIA R ET AL: "strain BPM3, a potential biocontrol agent isolated from a natural hot water spring of Assam, India", MICROBIOLOGICAL RESEARCH, FISCHER, JENA, DE, vol. 166, no. 3, 1 March 2010 (2010-03-01), pages 216 - 225, XP028153956, ISSN: 0944-5013, [retrieved on 20100406], DOI: 10.1016/J.MICRES.2010.03.002 *
SHANMUGAIAH V ET AL: "Optimization of cultural conditions for production of chitinase by Bacillus laterosporous MML2270 isolated from rice rhizosphere soil", AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 15, August 2008 (2008-08-01), pages 2562 - 2568, XP002699911, ISSN: 1684-5315 *

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WO2013050867A4 (en) 2013-12-19
WO2013050867A2 (en) 2013-04-11

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