WO2013009818A3 - High-performance ketol-acid reductoisomerases - Google Patents

High-performance ketol-acid reductoisomerases Download PDF

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Publication number
WO2013009818A3
WO2013009818A3 PCT/US2012/046185 US2012046185W WO2013009818A3 WO 2013009818 A3 WO2013009818 A3 WO 2013009818A3 US 2012046185 W US2012046185 W US 2012046185W WO 2013009818 A3 WO2013009818 A3 WO 2013009818A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
ketol
kari
performance
recombinant microorganisms
Prior art date
Application number
PCT/US2012/046185
Other languages
French (fr)
Other versions
WO2013009818A2 (en
Inventor
Peter Meinhold
Doug Lies
Stephanie Porter-Scheinman
Christopher Smith
Christopher Snow
Sabine Bastian
Sebastian Schoof
Original Assignee
Gevo, Inc.
California Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/303,884 external-priority patent/US20120190089A1/en
Application filed by Gevo, Inc., California Institute Of Technology filed Critical Gevo, Inc.
Priority to US14/131,984 priority Critical patent/US20140295513A1/en
Publication of WO2013009818A2 publication Critical patent/WO2013009818A2/en
Publication of WO2013009818A3 publication Critical patent/WO2013009818A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01086—Ketol-acid reductoisomerase (1.1.1.86)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
PCT/US2012/046185 2011-07-11 2012-07-11 High-performance ketol-acid reductoisomerases WO2013009818A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/131,984 US20140295513A1 (en) 2011-07-11 2012-07-11 High-Performance Ketol-Acid Reductoisomerases

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201161506564P 2011-07-11 2011-07-11
US201161506562P 2011-07-11 2011-07-11
US61/506,564 2011-07-11
US61/506,562 2011-07-11
US201161510618P 2011-07-22 2011-07-22
US61/510,618 2011-07-22
US13/303,884 2011-11-23
US13/303,884 US20120190089A1 (en) 2008-10-31 2011-11-23 Engineered microogranisms capable of producing target compounds under anaerobic conditions

Publications (2)

Publication Number Publication Date
WO2013009818A2 WO2013009818A2 (en) 2013-01-17
WO2013009818A3 true WO2013009818A3 (en) 2013-04-25

Family

ID=47506882

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/046185 WO2013009818A2 (en) 2011-07-11 2012-07-11 High-performance ketol-acid reductoisomerases

Country Status (1)

Country Link
WO (1) WO2013009818A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202008013TA (en) * 2016-04-06 2020-10-29 Immatics Biotechnologies Gmbh Novel peptides and combination of peptides for use in immunotherapy against aml and other cancers
CN114457122B (en) * 2020-05-13 2023-06-20 安徽华恒生物科技股份有限公司 Recombinant microorganism for producing L-valine as well as construction method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
US20100143997A1 (en) * 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
US20100197519A1 (en) * 2007-12-20 2010-08-05 E. I. Du Pont De Nemours And Company Ketol-acid reductoisomerase using nadh

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
US20100197519A1 (en) * 2007-12-20 2010-08-05 E. I. Du Pont De Nemours And Company Ketol-acid reductoisomerase using nadh
US20100143997A1 (en) * 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 5 September 2006 (2006-09-05), COPELAND ET AL.: "ketol-acid reductoisomerase [Shewanella sp. MR-4]", accession no. P000446.1. *
WEGMANN ET AL.: "Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. Cremoris MG1363.", JOURNAL OF BACTERIOLOGY., vol. 189, no. 8, 16 February 2007 (2007-02-16), pages 3256 - 3270 *

Also Published As

Publication number Publication date
WO2013009818A2 (en) 2013-01-17

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