WO2012175513A1 - Thienylpyri(mi)dinylpyrazole - Google Patents

Thienylpyri(mi)dinylpyrazole Download PDF

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Publication number: WO2012175513A1
Authority: WO; WIPO (PCT)
Prior art keywords: butyl; amino; iso; alkyl; methyl
Prior art date: 2011-06-20

Application number

PCT/EP2012/061741

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English (en)

French (fr)

Inventor

Alexander Sudau

Julia Neumann

Jürgen BENTING

Original Assignee

Bayer Intellectual Property Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2011-06-20

Filing date

2012-06-19

Publication date

2012-12-27

2012-06-19 Application filed by Bayer Intellectual Property Gmbh filed Critical Bayer Intellectual Property Gmbh

2012-12-27 Publication of WO2012175513A1 publication Critical patent/WO2012175513A1/en

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0 *C(C(c1c(*)[n](*)nc1-c1c(*)[s]c(*)c1*)=*)=C(*)N=C(*)I Chemical compound *C(C(c1c(*)[n](*)nc1-c1c(*)[s]c(*)c1*)=*)=C(*)N=C(*)I 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
- C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/04—Ortho-condensed systems

Definitions

the invention relates to novel Thienylpyri(mi)dinylpyrazole and their agrochemically active salts, to their use and to methods and compositions for controlling phytopathogenic harmful fungi in and/or on plants or in and/or on seed of plants and for reducing mycotoxins in plants and parts of the plants, to processes for preparing such compounds and compositions and treated seed and also to their use for controlling phytopathogenic harmful fungi in agriculture, horticulture, forestry, in animal husbandry, in the protection of materials, in the domestic and hygiene field and for the reduction of mycotoxins in plants and parts of the plants.
Thienylpyri(mi)dinylpyrazole solve at least in some aspects the problems mentioned above and are suitable for use as crop protection agents, in particular as fungicides.
Some Arylazoles are already known as pharmaceutically active compounds (see for example WO 1998/52937, EP-A 1 553 096, WO 2004/29043, WO 1998/52940, WO 2000/31063, WO 1995/31451, WO 2002/57265 and WO 2000/39116), but not their surprising fungicidal activity.
the invention provides compounds of the formula (I),
R 1 represents Ci-C6-alkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, Ci-C6-alkoxy, C3-C6-cycloalkyl, C3-C8- allenyl, Cs-Cs-trialkylsilyl, C4-C8-cycloalkenyl, Ci-C6-alkoxy-Ci-C6-alkyl, acyloxy-Ci-C6-alkyl, heteroaryl-Ci-Ce-alkyl, aryl-Ci-Ce-alkyl, Ci-C 6 -alkylthio-Ci-C 6 -alkyl, Ci-C 4 -alkyl-C(0)-Ci-C 6 - alkyl, C3-C 6 -cycloalkyl-C(0)-Ci-C 4 -alkyl, heterocyclyl-C(0)-Ci-C 4 -alkyl, Ci-C 4
R 2 represents H, cyano, halogen or represents Ci-C6-alkyl, C3-C6-cycloalkyl, heterocyclyl, C 2 -C8-alkenyl, C 2 -C8-alkinyl, Ci-Cs-alkoxy, C2-C8-alkynyloxy, Ci-Cs-alkylthio, Cs-Cs-trialkylsilyl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of halogen, cyano, amino, dimethylamino or methoxy,
R 4 and R 5 represents independently of each other H, F, CI, Br, I, cyano, nitro, OH, SH, or represents Ci-C6-alkyl, C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, C6-Ci 4 -aryl, C 1 -C4- alkoxy, 0-(C 6 -Ci 4 -aryl), S-(Ci-C 4 -alkyl), S(0)-(Ci-C 6 -alkyl), C(0)-(Ci-C 6 -alkyl), C(0)-(Ci-C 6 -alkyl), C 3 -C 8 - trialkylsilyl, heteroaryl, heterocyclyl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of R 16 or form together with the carbon atoms, which they are attached to, an optionally mono- or multi identical
R 6 represents Ci-C6-alkyl, H, halogen or represents Ci-C6-alkyl, Cs-Cs-cycloalkyl, C 2 -C8-alkenyl, C 2 -C8-alkinyl, C(0)-Ci-C6-alkyl, Ci-Cs- alkoxy, Ci-Cs-alkylthio, Ci-Cs-halogenalkoxy, Ci-C6-alkylsulphenyl, Ci-C6-alkylthio, C3-C8- trialkylsilyl, C6-Cio-aryl, heteroaryl, heterocyclyl, C6-Cio-aryloxy, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of R 16
R 7 represents H, halogen, cyano, nitro, Ci-C6-alkyl, C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, C5-Cio-aryl, heteroaryl, heterocyclyl, or R 6 and R 7 together can form a 5- to 7-membered ring which can optionally be substituted with one or more substituents selected from: Ci-C i-alkyl, Ci-C i-halogenoalkyl, Ci-C i-alkoxy, C1-C4- halogenoalkoxy, halogen, hydroxyl, amino, cyano or nitro, whereas the cycle consists of carbon atoms but may also contain 1 to 4 heteroatoms selected from oxygen, sulphur or NR 19 ,
R 8 represents H, halogen, cyano, Ci-C6-alkyl, C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, Ce- Cio-aryl, heteroaryl, heterocyclyl,
R 9 and R 10 represent H, C(S)R 14 , C(0)R 14 , SO2R 14 , C(0)OR 14 , OR 14 oder C(0)NR 14 R 15 or represents Ci-C6-alkyl , C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, C3-C8-cycloalkyl, heterocyclyl or heteroaryl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, OH, cyano, Ci-C6-alkyl, 0-C(0)R 17 , 0-P(0)(OR 17 ) 2 , 0-B(OR 17 ) 2 or 0-(Ci-C 4 -alkyl),
R 11 and R 20 represent H, C(S)R 12 , C(0)R 12 , SO2R 12 , C(0)OR 12 , OR 12 or C(0)NR 12 R 13 or represents Ci-C6-alkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, C3-C8-cycloalkyl, C6-Ci 4 -aryl, heterocyclyl or heteroaryl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, OH, cyano, Ci-C6-alkyl, 0-C(0)R 17 , O- P(0)(OR 17 ) 2 , 0-B(OR 17 ) 2 or 0-(Ci-C 4 -alkyl),
R 12 and R 13 represent H
Ci-C6-alkyl C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, C3-C8-cycloalkyl, Ce-Cw- aryl, heterocyclyl oder heteroaryl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, I, OH, carbonyl, cyano, Ci-C6-alkyl or Ci-C i-alkoxy, R 14 and R 15 represent H
Ci-C6-alkyl C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, Cs-Cs-cycloalkyl, Ce-Cw- aryl, benzyl, phenethyl, phenoxymethyl, heterocyclyl oder heteroaryl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, I, OH, carbonyl, cyano, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec -butyl, tert- butyl, n-pentyl, cyclopropyl, or methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy, tert-butoxy, methylsulfanyl, nitro,
R 16 represents OH, F, CI, Br, I, cyano, NH-C(0)R 17 , NR 17 R 18 , C(0)R 17 , C(0)OR 17 , C(0)NR 17 R 18 , or represents Ci-C6-alkyl, C2-C6-alkenyl, C2-C6-alkinyl, Cs-Cs-cycloalkyl, Ci-C4-alkoxy, C1-C4- alkylthio, 0-(C 3 -C 8 -cycloalkyl), S-(C 3 -C 8 -cycloalkyl), C 6 -Ci 4 -aryl, 0-(C 6 -Ci 4 -aryl), S-(C 6 -Ci 4 - aryl), heterocyclyl or heteroaryl, each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, I, OH, carbonyl, cyano, Ci
R 17 and R 18 represents Ci-C6-alkyl, C3-C6-cycloalkyl, C 2 -C6-alkenyl, C 2 -C6-alkinyl, benzyl, aryl each of which is optionally mono- or polysubstituted by identical or different substituents from the group consisting of F, CI, Br, OH, cyano or represents H,
R 19 represents H, Ci-Ce-alkyl, C 3 -C 6 -cycloalkyl, C 2 -C 6 -alkenyl, C 2 -C 6 -alkinyl, C(S)R 14 , C(0)R 14 , and also agrochemically active salts thereof.
the invention also provides the use of the compounds of the formula (I) as fungicides.
Thienylpyri(mi)dinylpyrazole of the formula (I) according to the invention and also their agrochemically active salts are highly suitable for controlling phytopathogenic harmful fungi and for the reduction of mycotoxins.
the compounds according to the invention mentioned above have in particular strong fungicidal activity and can be used both in crop protection, in the domestic and hygiene field, in the protection of materials and for the reduction of mycotoxins in plants and parts of the plants.
the compounds of the formula (I) can be present both in pure form and as mixtures of various possible isomeric forms, in particular of stereoisomers, such as E and Z, threo and erythro, and also optical isomers, such as R and S isomers or atropisomers, and, if appropriate, also of tautomers.
stereoisomers such as E and Z, threo and erythro, and also optical isomers, such as R and S isomers or atropisomers, and, if appropriate, also of tautomers.
optical isomers such as R and S isomers or atropisomers
CH 2 CH CH 2 , -CH 2 C ⁇ CH , -C ⁇ CH or R 6 and R 7 together can form a 5- to 7-membered ring which can optionally be substituted with one or more substituents selected from: methyl, ethyl, trifluoromethyl, methoxy, trifluoromethoxy, F, CI, Br, hydroxyl, amino, cyano or nitro, whereas the cycle consists of carbon atoms but may also contain 1 to 4 heteroatoms selected from oxygen, sulphur or NR 19 ,
R 9 and R 10 represent H, C(S)R 14 , C(0)R 14 , SO2R 14 , C(0)OR 14 , OR 14 oder C(0)NR 14 R 15 , R 11 and R 20 represent H, C(S)R 12 , C(0)R 12 , SO2R 12 , C(0)OR 12 , OR 12 oder C(0)NR 12 R 13 or represents methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, n-pentyl, 0-C(0)Me, 0-C(0)Et, 0-P(0)(OMe) 2 , 0-B(OMe) 2 , 0-B(OEt) 2 oder , methoxy, ethoxy, n- propoxy, iso-propoxy, n-butoxy, tert-butoxy substituted methyl, ethyl, n-propyl, iso-propyl,
R 12 and R 13 represent H
X 1 represents CH
R 1 represents H, methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, allyl, 3-methylbut-2-en-l-yl, prop-2-yn-l-yl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, tetrahydro-2H-pyran-2-yl, tetrahydrofuran-3-yl, oxetan-3-yl, thietan-3-yl, tetrahydro-2H-pyran-3-yl, tetrahydro-2H-pyran-4- yl, 2,2-difluoroethyl, l,3-difluoropropan-2-yl, 2-chloroethyl, 2-fluoroethyl, 2-
R 2 represents H, Methyl, Ethyl, n-propyl, methoxy, ethoxy, methylsulfanyl, methoxymethyl, difluoromethyl, 2-hydroxypropan-2-yl, hydroxymethyl, 2-hydroxyethyl, 2-cyanoethyl,
R 3 represents H, amino, formamido, acetylamino, n-propionylamino, isobutyrylamino, (2- methylbutanoyl)amino, (3-methylbutanoyl)amino, (3,3-dimethylbutanoyl)amino,
R 7 represents H, F, CI, Methyl
R 8 represents H, CI
R 1 represents H, methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, cyclopropyl, 2,2-difluoroethyl, l,3-difluoropropan-2-yl, 2-chloroethyl, 2-cyanoethyl, 1 -cyanopropan-2-yl, 2-methoxyethyl, 2- ethoxyethyl, cyclopropylmethyl,
R 2 represents H, methyl
R 3 represents H, acetylamino, n-propionylamino, isobutyrylamino, (2-methylbutanoyl)amino, (3- methylbutanoyl)amino, (methoxyacetyl)amino, (lactoylamino), (2-hydroxy-2- methylpropanoyl)amino, (phenyl acetyl) amino , ( cy c 1 opr opy lac etyl) amino ,
R 1 represents H, methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, cyclopropyl, 2,2-difluoroethyl, l,3-difluoropropan-2-yl, 2-chloroethyl, 2-cyanoethyl, 1 -cyanopropan-2-yl, 2-methoxyethyl, 2- ethoxyethyl, cyclopropylmethyl where the other substituents have one or more of the meanings mentioned above, and to the agrochemically active salts thereof.
R 3 represents H, acetylamino, n-propionylamino, isobutyrylamino, (2-methylbutanoyl)amino, (3- methylbutanoyl)amino, (methoxyacetyl)amino, (lactoylamino), (2-hydroxy-2- methylp ro p an o yl) amin o , (phenyl ac etyl) amin o , ( cyc l op ro py l ac ety l) amino , (cyclopropylcarbonyl)amino, [(2-methylcyclopropyl)carbonyl] amino, (cyclobutylcarbonyl)amino, methacryloylamino, (3-methylbut-2-enoyl)amino, (benzoylamino), (3 -thienylcarbonyl)amino, (2-thienylcarbon
R 4 represents H
R 5 represents H, F
R 6 represents H, F, CI, Methyl
R 7 represents H
R 8 represents H
the compounds of the formula (I) have acidic or basic properties and can form salts, if appropriate also inner salts, or adducts with inorganic or organic acids or with bases or with metal ions. If the compounds of the formula (I) carry amino, alkylamino or other groups which induce basic properties, these compounds can be reacted with acids to give salts, or they are directly obtained as salts in the synthesis. If the compounds of the formula (I) carries hydroxyl, carboxyl or other groups which induce acidic properties, these compounds can be reacted with bases to give salts.
Suitable bases are, for example, hydroxides, carbonates, bicarbonates of the alkali metals and alkaline earth metals, in particular those of sodium, potassium, magnesium and calcium, furthermore ammonia, primary, secondary and tertiary amines having (Ci-C i)-alkyl groups, mono-, di- and trialkanolamines of (Ci-C i)-alkanols, choline and also chlorocholine.
the salts obtainable in this manner also have fungicidal properties.
inorganic acids are hydrohalic acids, such as hydrogen fluoride, hydrogen chloride, hydrogen bromide and hydrogen iodide, sulphuric acid, phosphoric acid and nitric acid, and acidic salts, such as NaHS04 and KHSO4.
Suitable organic acids are, for example, formic acid, carbonic acid and alkanoic acids, such as acetic acid, trifluoroacetic acid, trichloroacetic acid and propionic acid, and also glycolic acid, thiocyanic acid, lactic acid, succinic acid, citric acid, benzoic acid, cinnamic acid, oxalic acid, alkylsulphonic acids (sulphonic acids having straight-chain or branched alkyl radicals of 1 to 20 carbon atoms), arylsulphonic acids or aryldisulphonic acids (aromatic radicals, such as phenyl and naphthyl, which carry one or two sulphonic acid groups), alkylphosphonic acids (phosphonic acids having straight-chain or branched alkyl radicals of 1 to 20 carbon atoms), arylphosphonic acids or aryldiphosphonic acids (aromatic radicals, such as phenyl and naphthyl, which carry one or two phospho
Suitable metal ions are in particular the ions of the elements of the second main group, in particular calcium and magnesium, of the third and fourth main group, in particular aluminium, tin and lead, and also of the first to eighth transition group, in particular chromium, manganese, iron, cobalt, nickel, copper, zinc and others. Particular preference is given to the metal ions of the elements of the fourth period.
the metals can be present in various valencies that they can assume.
Optionally substituted groups may be mono- or polysubstituted, where in the case of polysubstitution the substituents may be identical or different.
substituents halogen: fluorine, chlorine, bromine and iodine
5-membered heteroaryl which contains one to four nitrogen atoms or one to three nitrogen atoms and one sulphur or oxygen atom
5-membered heteroaryl groups which, in addition to carbon atoms, may contain one to four nitrogen atoms or one to three nitrogen atoms and one sulphur or oxygen atom as ring members, for example (but not limited thereto) 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-pyrrolyl, 3-pyrrolyl, 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl, 3-isothiazolyl, 4-isothiazolyl, 5-isothiazolyl, 3- pyrazolyl, 4-pyrazolyl, 5-pyrazolyl, 2-oxazolyl, 4-oxazolyl, 5- oxazolyl, 2-thiazolyl, 4-thiazolyl, 5- thiazolyl, 2-imidazolyl, 4-imidazolyl, l,
5-membered heteroaryl which is attached via nitrogen and contains one to four nitrogen atoms, or benzo-fused 5-membered heteroaryl which is attached via nitrogen and contains one to three nitrogen atoms: 5-membered heteroaryl groups which, in addition to carbon atoms, may contain one to four nitrogen atoms and one to three nitrogen atoms, respectively, as ring members and in which two adjacent carbon ring members or a nitrogen and an adjacent carbon ring member may be bridged by a buta-l,3-dien-l,4-diyl group in which one or two carbon atoms may be replaced by nitrogen atoms, where these rings are attached to the skeleton via one of the nitrogen ring members, for example (but not limited thereto) 1-pyrrolyl, 1-pyrazolyl, 1,2,4-triazol-l -yl, 1 -imidazolyl, 1,2,3-triazol-l -yl, 1,3,4-triazol- 1-yl;
6-membered heteroaryl which contains one to four nitrogen atoms: 6-membered heteroaryl groups which, in addition to carbon atoms, may contain one to three or one to four nitrogen atoms as ring members, for example (but not limited thereto) 2-pyridinyl, 3-pyridinyl, 4-pyridinyl, 3-pyridazinyl, 4- pyridazinyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, 2-pyrazinyl, l,3,5-triazin-2-yl, l,2,4-triazin-3- yl and l,2,4,5-tetrazin-3-yl; benzo-fused 5-membered heteroaryl which contains one to three nitrogen atoms or one nitrogen atom and one oxygen or sulphur atom: for example (but not limited thereto) lH-indol-l -yl, lH-indol- 2-yl, lH-ind
the present invention furthermore relates to a process for preparing thienylpyri(mi)dinylpyrazole of the formula [I] according to the invention.
the Thienylpyri(mi)dinylpyrazole according to the invention of the formula [I] can be prepared in different ways. Below, the possible processes are firstly shown schematically and then described in detail. Unless otherwise indicated, the residues stated have the meanings given below the schemes.
the Thienylpyri(mi)dinylpyrazole according to the invention of the formula [I] can be produced by process A according to the following scheme.
Met 2 e.g. -B(OR*) 2
B(OR*) 2 e.g. -B(OiPr) 2 , -B(OH) 2 , -B(pinacolato)
Z l e.g. CI, Br, I, -OTos, -OMs, -OH
Z 2 e.g. CI, Br
a 7 R 15 , -OR 15
R a e.g. -NH-C(S)R 15 , -NH-C(0)R 15 , -NH C(0)OR 15 , -NH-C(0)NR 15 R 16 ,
PG e.g. tetrahydro-2H-pyran-2-yl, 2-(trimethylsilyl)ethoxy]methyl
Z 4 e.g. CH 3 , OAlkyl
Met 3 e.g. Sn(n-Bu) 3
a compound with the general formula [VII] is halogenated and a compound of the formula [V] is obtained. This is converted to a compound of the type [IV] by reaction with substrates of the type [VIII], whereby mixtures of pyrazole regioisomers can be formed. These can be separated into the individual regioisomers by common processes e.g. chromatographic processes.
a compound with the general formula [VII] is converted into a compound of the type [VI] by reaction with substrates of the type [VIII], whereby mixtures of pyrazole regioisomers can be formed.
Compounds of the formula [VI] can be halogenated whereas compounds of the type [IV] are obtained.
the compounds of the general formula [IV] can be reacted with substrates of the formula [IX-a] in a C-C coupling, whereby compounds of the formula [I] are obtained (Scheme 1).
the pyrazole compounds of the general formula [IV] can be converted into compounds of the type [III] by reaction with a boronic acid ester. These can be converted into compounds of the formula [I] by reaction with a substrate of the formula [X-a] in a C-C coupling reaction (Scheme 1).
compounds of the type [IV] can be converted into compounds of the formula [II] by reaction with a substrate of the formula [IX-b] in a C-C coupling reaction and subsequent deprotection. These compounds are likewise converted into the compounds of the type [I-a] by reaction with substrates of the formula [XI].
structures of the general formula [VI] can be obtained from alkynes of the general formula [XVI] by reaction with hydrazines.
the Intermediates of the general formula [XVI] can be formed by transition metal catalysed coupling of Acyl halides of the general formula [XIV] or by reaction of compounds of the general formula [XV] in the presence of carbon monoxide (scheme 2).
a compound with the general formula [XVII] can be reacted with a substrate of the formula [XVIII] in a C-C coupling reaction, whereby a compound with the formula [XIX] is formed. Next this compound is deprotected, whereby a compound of the general formula [XX] is obtained.
the pyrazole of the formula [XX] obtained is now reacted with substrates of the type [VIII], whereby the thienylpyri(mi)dinylpyrazole according to the invention of the formula [I-b] are obtained (Scheme 3).
a compound with the general formula [XXI] can be converted to a Weinreb amide with the formula [XXII]. This compounds is reacted with an acetylene in the presence of a strong base followed by treatment with hydrazine, whereby a pyrazole of the general formula [Vll-a] is obtained (Scheme 4).
the compounds of the formula [VIII] required for the reaction are commercially available or can be produced by literature methods (R. C. Larock, Comprehensive Organic Transformations, 2nd Edition, 1999, Wiley- VCH, p. 690 ff. and p. 1929 ff. and literature cited therein)
One method for the production of suitable compounds of the formula [VIII] (wherein R 1 in the case of an alkylation reaction e.g. stands for a substituted or unsubstituted alkyl or cycloalkyl residue), is for example the reaction of alcohols with methanesulphonyl chloride and triethylamine (Org. Lett.
dichloromethane chloroform, carbon tetrachloride
halogenated aromatic hydrocarbons e.g. chlorobenzene, dichlorobenzene
nitriles e.g. acetonitrile
carboxylic acid esters e.g. ethyl acetate
amides e.g. ⁇ , ⁇ -dimethylformamide, N,N-dimethylacetamide
the preferred solvents are dimethylformamide and acetonitrile.
bases which can be used for this reaction are for example lithium hexamethyldisilazide (LiHMDS), potassium carbonate, caesium carbonate and sodium hydride.
LiHMDS lithium hexamethyldisilazide
the preferred base is sodium hydride.
at least 1 equivalent of base is used.
an acylation reaction with substrates of the formula [VIII] (wherein in R 1 a carbonyl group is directly bound to Z 1 )
a base e.g. pyridine, diisopropylethylamine, triethylamine or commercially available polymeric acid scavengers
the starting material is a salt, at least two equivalents of the acid scavenger are needed.
pyridine is used as the solvent, analogously to the literature described, the addition of a further base can in some cases be omitted (EP-A-1 000 062).
the reaction is normally effected at temperatures of 0°C - 100°C and preferably at 20°C - 30°C, but it can also be effected at the reflux temperature of the reaction mixture.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between a few minutes and 48 hours.
the compounds [VI] or [IV] are separated from the reaction mixture by one of the usual separation techniques.
the compounds of the formula [VI] and [IV], wherein R 1 does not stand for hydrogen can be obtained as pure regioisomers or as a mixture of both possible regioisomers (wherein the group R 1 can occupy both positions on the N atom of the pyrazole).
these can be purified by physical methods (such as for example crystallization or chromatography methods) or can optionally also be used in the next step without prior purification.
halogenated pyrazoles of the formula [V] are either commercially available or can be produced by literature methods.
One method for the production of suitable halogenated pyrazoles is for example the bromination of corresponding pyrazoles [VII] (e.g. described in EP-A 1382 603) by reaction with N-bromosuccinimide in acetic acid.
Step (V3) One possibility for the synthesis of compounds of the formula [III] is shown in Scheme 1.
Compounds of the formula [III] can be produced by described methods e.g. via reaction of the halopyrazoles [IV] with boronic acid esters such as for example bispinacolatodiboron (4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi-l,3,2-dioxaborolane) in the presence of a catalyst such as for example l, -bis(diphenyl-phosphino)ferrocene-palladium(II) dichloride in the presence of a base and a suitable solvent (see US 2009/0018156, WO 2007/024843 or EP-A 1 382 603).
boronic acid esters such as for example bispinacolatodiboron (4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi-l,3,2-dioxaborolane
a catalyst such as for example l, -bis(
solvent all common solvents inert under the reaction conditions, such as for example sulphoxides (e.g. dimethyl sulphoxide), cyclic ethers (e.g. dioxan) and amides (e.g. N,N- dimethylformamide) can be used and the reaction can be effected in mixtures of two or more of these solvents.
the preferred solvents are dimethyl sulphoxide and dioxan.
the reaction will normally be effected at temperatures of 80°C - 120°C, and the preferred reaction temperature is about 85°C - 90°C.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between one hour and 16 hours.
compounds of the formula [III] can be produced by metallation of the halogenated pyrazoles [IV] with bases such as for example n-butyllithium and reaction with boronic acid esters such as for example trimethyl borate and subsequent reaction of the pyrazole-boronic acid obtained with pinacol (see e.g. J. Het. Chem. 2004, 41, 931-940 or EP-A 1 382 603 and WO 2007/16392).
Compounds of the formula [I] can also be produced for example by coupling of the halopyrazoles [IV] with metallated heterocycles of the formula [IX-a] (wherein Met 1 stands for a tin-compound such as for example Sn(n-Bu)s) in the presence of a catalyst, if necessary an inorganic or organic halide salt, if necessary a ligand and a suitable solvent at suitable temperatures by known literature procedures (see Synthesis 1992, 803-815).
compounds of the formula [IX-al] can also be prepared by other known literature methods.
One method for the production of suitable heterocycles [IX-al] is the metallation of the halopyridine [XXIII] with a base (such as for example n-butyllithium) in a solvent (such as for example diethyl ether or tetrahydrofuran) and subsequent reaction with a boronic acid ester (such as for example B(i-PrO)3 or B(OMe)3) and pinacol by known literature methods (Synthesis 2004, 4, 469-483 and literature described therein) (Scheme 6).
a base such as for example n-butyllithium
solvent such as for example diethyl ether or tetrahydrofuran
a boronic acid ester such as for example B(i-PrO)3 or B(OMe)3
compounds of the type [IX-b] can be synthetised according to literature described methods (WO 2011/042389) by reaction of the respective haloheterocycle precursor (replacement of Met 2 by CI, Br, I in [IX-b]) with bispinacolatodiboron in the presence of a catalyst.
haloheterocycles of the formula [XXIV] are commercially available or can be produced by literature procedures.
One method for the production of suitable haloheterocycles [IX- al] is the reaction of haloheterocycles of the formula [XXIV] with hexaalkylditin compounds (such as for example 1 , 1 , 1 ,2,2,2-hexabutylditin) in the presence of a catalyst (such as for example bis(triphenylphosphine)palladium(II) acetate), if necessary a fluoride ion source (such as for example tetrabutylammonium fluoride) and a solvent (such as for example tetrahydrofuran or diethyl ether) by methods described in the literature (WO 2003/095455 or WO 2007/104538) (Scheme 7).
Scheme 7 Scheme 7
ALK C r C 5 Alkyl [, ⁇ ]
compounds of the formula [IX-a2] can also be prepared by other known literature methods.
One method for the production of suitable haloheterocycles [IX-a2] is the metallation of the halopyridine [XXIV] using a metallation reagent (an alkyllithium compound such as for example n-butyllithium or a Grignard reagent such as for example isopropylmagnesium chloride) in a solvent (such as for example diethyl ether or tetrahydrofuran) and subsequent reaction with a trialkyltin halogen compound (such as for example BusSnCl) by known literature methods (WO 2008/008747 or Tetrahedron 1994, 275-284 and literature described therein) (Scheme 8).
a metallation reagent an alkyllithium compound such as for example n-butyllithium or a Grignard reagent such as for example isopropylmagnesium chloride
a solvent such
ALK C r C 5 Alkyl
solvents inert under the reaction conditions such as for example cyclic and acyclic ethers (diethyl ether, dimethoxymethane, diethylene glycol dimethyl ether, tetrahydrofuran, dioxan, diisopropyl ether, tert-butyl methyl ether), aromatic hydrocarbons (e.g. benzene, toluene, xylene), amides (e.g. dimethylformamide, dimethyl-acetamide, N-methylpyrrolidone) and sulphoxides (e.g. dimethyl sulphoxide) can be used or the reaction can be performed in mixtures of two or more of these solvents.
the preferred solvent is dimethylformamide.
Halide salts for the reaction of compounds of the formula [IX-a] which are preferably used in the process according to the invention are for example copper halides (e.g. CuBr or Cul), caesium halides (CsF) and tetraalkylammonium halides (TBAF).
copper halides e.g. CuBr or Cul
CsF caesium halides
TBAF tetraalkylammonium halides
the halide salts are preferably used in the process according to the invention in a proportion of 1 to 400 mol.%, based on the organic tin compound. However, mixtures of the halide salts can also be used in proportions of 1-400 mol.%. The addition of a mixture of copper iodide and caesium fluoride in proportions of 1- 200 mol.% is particularly preferable.
catalysts for the reaction of compounds of the formula [IX-a] with halogenated pyrazoles of the formula [IV] the same catalysts can be used as are described below for the production of the compounds of the formula [I], by reaction of the compounds of the formula [III] and [X-a] described for step V5.
the quantity of catalyst, based on the heteroaromatics [IX-a] bearing the leaving group Met 1 is preferably 0.001 to 0.5 mol.%> and particularly preferably 0.01 to 0.2 mol.%>.
the catalyst can contain phosphorus-containing or arsenic-containing ligands or phosphorus- containing or arsenic-containing ligands can be added separately to the reaction mixture.
phosphorus-containing ligands preferably tri-n-alkylphosphanes, triarylphosphanes, dialkylaryl- phosphanes, alkyldiarylphosphanes and/or heteroarylphosphanes, such as tripyridylphosphane and trifurylphosphane, wherein the three substituents on the phosphorus can be the same or different, can be chiral or achiral and wherein one or more substituents can link the phosphorus groups of several phosphanes, wherein one part of this linkage can also be a metal atom, are suitable.
phosphanes such as triphenylphosphane, tri-tert-butylphosphane and tricyclohexyl-phosphane.
arsenic-containing ligands for example tri-n-alkylarsanes and triarylarsanes, wherein the three substituents on the arsenic can be the same or different, are suitable.
the total concentration of ligands, based on the heteroaromatics [IX-a] bearing the leaving group Met 1 is preferably up to 1 mol.%, particularly preferably 0.01 to 0.5 mol.%.
the educts, the solvent, the base, the halide salt, the catalyst and if necessary the ligand are thoroughly mixed and reacted preferably at a temperature of 0°C- 200°C, particularly preferably at 60-150°C.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between a few minutes and 48 hours.
the reaction can also be run such that the various reactants are metered in a controlled manner in the course of the reaction, whereby different metering variants are possible.
the processes according to the invention are in general performed under normal pressure. However it is also possible to operate under increased or reduced pressure.
the reaction is in general performed using a blanket gas such as for example argon or nitrogen.
the molar reactant ratio of the halopyrazole [IV] to the organotin compound [IX-a2] is preferably 0.9 to 2.
the catalyst arising as a solid is removed by filtration, the crude product freed from the solvent or solvents and then purified by methods known to those skilled in the art and appropriate for the particular product, e.g. by recrystallization, distillation, sublimation, zone melting, melt crystallization or chromatography.
compounds of the formula [II] can be produced by coupling of the pyrazoleboronic acids [III] with heterocycles of the formula [X-b].
Compounds of the formula [X-a] (wherein X 1 stands for C-H) are commercially available or can be produced by literature procedures (Scheme 9).
One method for the production of suitable haloheterocycles [X-al] is the reaction of the pyridine N-oxides with halogenating agents (e.g. PC1 3 , POCI3, SOCb or methanesulphonyl chloride) (see Bioorg. Med. Chem. Lett. 2007, 17, 7, 1934-1937).
the pyridine N-oxides [XXV] are known or can be produced by oxidation of the corresponding pyridines (e.g. with H2O2, H2O2 + methyltrioxorhenium, m-chloroperoxybenzoic acid, dimethyl- dioxirane or H2O2 + manganese tetrakis(2,6-dichlorophenyl)porphyrin) by procedures described in the literature (ARKIVOC 2001 (i) 242-268 and references contained therein).
aminoheterocycles [XXVII] (wherein X 1 stands for C-H) are known or can be produced by removal of the N-BOC protective group from compounds of the formula [X-b-1] by procedures described in the literature (Aust. J. Chem. 1982, 35, 10, 2025-2034 and references contained therein).
Compounds of the formula [X-b] (wherein X 1 stands for C-H) are commercially available or can be produced by literature methods (Scheme 12).
One method for the production of suitable N-Boc- haloheterocycles [X-b-1] is the reaction of suitable acids (e.g.
the carboxylic acids [XXIX] are known or can be produced from commercially available precursors by procedures described in the literature (see e.g. EP-A 1 650 194), for example from the commercially available pyridine-2-carboxylic acid by reaction with thionyl chloride in dimethylformamide.
compounds of the general formula [L] can also be produced by oxidation of commercially available 4-halo-2-methyl-pyridine derivatives by known literature procedures (Aust. J. Chem. 1982, 35, 2025-2034).
solvents inert under the reaction conditions such as for example alcohols (e.g. methanol, ethanol, 1-propanol, 2- propanol, ethylene glycol, 1-butanol, 2-butanol, tert-butanol), cyclic and acyclic ethers (diethyl ether, dimethoxymethane, di ethylene glycol dimethyl ether, tetrahydrofuran, dioxan, diisopropyl ether, tert-butyl methyl ether), aromatic hydrocarbons (e.g.
alcohols e.g. methanol, ethanol, 1-propanol, 2- propanol, ethylene glycol, 1-butanol, 2-butanol, tert-butanol
cyclic and acyclic ethers diethyl ether, dimethoxymethane, di ethylene glycol dimethyl ether, tetrahydrofuran, dioxan, diiso
benzene, toluene, xylene hydrocarbons (e.g. hexane, iso-hexane, heptane, cyclohexane), ketones (e.g. acetone, ethyl methyl ketone, iso-butyl methyl ketone), nitriles (e.g. acetonitrile, propionitrile, butyronitrile) and amides (e.g. dimethyl-formamide, dimethylacetamide, N-methylpyrrolidone) and water can be used or the reaction can be effected in mixtures of two or more of these solvents.
the preferred solvent is dioxan.
Bases which are preferably used in the process according to the invention are alkali and alkaline earth metal hydroxides, alkali and alkaline earth metal carbonates, alkali metal hydrogen carbonates, alkali and alkaline earth metal acetates, alkali and alkaline earth metal alcoholates, and primary, secondary and tertiary amines.
Preferred bases are alkali metal carbonates such as for example caesium carbonate, sodium carbonate and potassium carbonate.
the base is preferably used in a proportion of 100 to 1000 mol.%, based on the aromatic boronic acid.
the preferred proportion is 600 to 800 mol.%.
catalysts for example palladium metal, palladium compounds and/or nickel compounds can be used.
the catalysts can also be applied onto a solid carrier, such as activated charcoal or aluminium oxide.
catalysts are tetrakis(triphenylphosphine)-palladium, bis(triphenylphosphine)-palladium dichloride and bis-(diphenylphosphino)ferrocenepalladium dichloride.
the palladium compound can also be generated in situ, such as for example palladium(II) acetate from palladium(II) chloride and sodium acetate.
the quantity of catalyst, based on the heteroaromatics [X-a] and [X-b] bearing the leaving group Z 2 , is preferably 0.001 to 0.5 mol.% and particularly preferably 0.01 to 0.2 mol.%.
the catalyst can contain phosphorus-containing ligands or phosphorus-containing ligands can be added separately to the reaction mixture.
phosphorus-containing ligands are tri-n-alkylphosphanes, triarylphosphanes, dialkylarylphosphanes, alkyldiarylphosphanes and/or heteroarylphosphanes, such as tripyridylphosphane and trifurylphosphane, wherein the three substituents on the phosphorus can be the same or different and wherein one or more substituents can link the phosphorus groups of several phosphanes, wherein one part of this linkage can also be a metal atom.
Particularly preferable are phosphanes such as triphenylphosphane, tri-tert- butylphosphane and tricyclohexylphosphane.
the total concentration of phosphorus-containing ligands, based on the heteroaromatics [X-a] and [X-b] bearing the leaving group Z 2 is preferably up to 1 mol.%, particularly preferably 0.01 to 0.5 mol.%.
the educts, the solvent, the base, the catalyst and if appropriate the ligand are thoroughly mixed and reacted preferably at a temperature of 0°C - 200°C, particularly preferably at 100-170°C.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between a few minutes and 48 hours.
the reaction can also be run such that the various reactants are metered in a controlled way in the course of the reaction, different metering variants being possible.
the molar reactant ratio of the heteroaromatic [X-a] and [X-b] to the organoboron compound [III] is preferably 0.9 to 1.5.
the processes according to the invention are generally performed under normal pressure. It is however also possible to operate under increased or reduced pressure.
the reaction is generally performed with the use of a blanket gas such as for example argon or nitrogen.
the catalyst arising as a solid is removed by filtration, the crude product freed from the solvent or solvents and then purified by methods known to those skilled in the art and appropriate for the particular product, e.g. by recrystallization, distillation, sublimation, zone melting, melt crystallization or chromatography.
a compound with the general formula [I-a] can be synthesized, analogously to procedures described in the literature (see e.g. WO 2004/052880 and e.g. T.W. Greene, P. G. M. Wuts, Protective Groups in Organic Synthesis, 1999, John Wiley & Sons, Inc.), by a coupling reaction of a compound with the corresponding general formula [II] with a substrate of the general formula [XI] (with Z 3 e.g.
solvent all usual solvents inert under the reaction conditions, such as for example cyclic and acyclic ethers (e.g. diethyl ether, tetrahydrofuran, dioxan), aromatic hydro-carbons (e.g. benzene, toluene, xylene), halogenated hydrocarbons (e.g. dichloromethane, chloroform, carbon tetrachloride), halogenated aromatic hydrocarbons (e.g. chlorobenzene, dichlorobenzene) and nitriles (e.g. acetonitrile) can be used or the reaction can be effected in mixtures of two or more of these solvents.
the preferred solvents are tetrahydrofuran and dichloromethane.
At least one equivalent of an acid scavenger / a base e.g. Hiinig base, triethylamine or commercially available polymeric acid scavengers
a base e.g. Hiinig base, triethylamine or commercially available polymeric acid scavengers
the starting material is a salt, at least two equivalents of the acid scavenger are needed.
the reaction is normally effected at temperatures of 0°C - 100°C and preferably at 20°C - 30°C, but it can also be effected at the reflux temperature of the reaction mixture.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between a few minutes and 48 hours.
Suitable coupling reagents are for example peptide coupling reagents (for example, N-(3-dimethyl- aminopropyl)-N'-ethyl-carbodiimide mixed with 4-dimethylamino-pyridine, N-(3-dimethylamino- propyl)-N'-ethyl-carbodiimide mixed with 1-hydroxy-benzotriazole, bromo-tripyrrolidino- phosphonium hexafluorophosphate, 0-(7-azabenzotriazol-l-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate, etc.).
peptide coupling reagents for example, N-(3-dimethyl- aminopropyl)-N'-ethyl-carbodiimide mixed with 4-dimethylamino-pyridine, N-(3-dimethylamino- propyl)-N'-
a base such as for example triethylamine or Hiinig base can be used in the reaction.
solvent all usual solvents inert under the reaction conditions, such as for example alcohols (e.g. methanol, ethanol, propanol), cyclic and acyclic ethers (e.g. diethyl ether, tetrahydrofuran, dioxan), aromatic hydrocarbons (e.g. benzene, toluene, xylene), halogenated hydrocarbons (e.g. dichloromethane, chloroform, carbon tetrachloride), halogenated aromatic hydrocarbons (e.g. chlorobenzene, dichlorobenzene), nitriles (e.g. acetonitrile) and amides (e.g.
alcohols e.g. methanol, ethanol, propanol
cyclic and acyclic ethers e.g. diethyl ether, tetrahydrofuran, dioxan
aromatic hydrocarbons e.g. benzene
N,N- dimethylformamide, ⁇ , ⁇ -dimethylacetamide can be used or the reaction can be performed in mixtures of two or more of these solvents.
the preferred solvent is dichloromethane.
the reaction is normally performed at temperatures of 0°C - 100°C and preferably at 0°C - 30°C, but it can also be performed at the reflux temperature of the reaction mixture.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between a few minutes and 48 hours.
the compounds [I-a] are separated from the reaction mixture by one of the usual separation techniques. If necessary, the compounds are purified by recrystallization, distillation or chromatography.
Step (V7) One possibility for the synthesis of compounds of the formula [XIII] is shown in Scheme 2.
the reaction temperature can be varied from room temperature to the boiling point of the reaction mixture.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but is generally between a couple of minutes and 48 hours.
compounds of the general formula [XIII] can be obtained according to known literature methods (J. Org. Chem. 2001, 66, 24, 8000-8009) by reacting an ester compound of general formula [XII] (where Z 3 stands for OAlkyl) with a methylketone of the general formula CH3COR 2 in the presence of a base (e.g. sodium hydride, lithium diisopropylamide or potassium tert. butanolate) and a solvent.
a base e.g. sodium hydride, lithium diisopropylamide or potassium tert. butanolate
Typical solvents include alcohols (e.g. ethanol) or ethers (e.g. THF).
compounds of the general formula [XIII] can be obtained according to known literature methods ⁇ Bioorg. Med. Chem. Lett. 2004 , 14, 2, 343-346) by reacting an methylketone general formula [XII] (where Z 3 stands for CH3) with an carboxylic acid ester of the general formula R 2 COOAlkyl in the presence of a base (e.g. sodium hydride, lithium diisopropylamide or potassium tert. butanolate) and a solvent.
a base e.g. sodium hydride, lithium diisopropylamide or potassium tert. butanolate
Typical solvents include alcohols (e.g. ethanol) or ethers (e.g. THF).
compounds [XII] are removed from the reaction mixture using one of the customary separation techniques. If required, the compounds are purified by recrystallisation, distillation or chromatography, or they can, if appropriate, also be used for the next step without prior purification.
Compounds of the general formula [VII] may be obtained, according to known literature methods (Tetrahedron 1995, 51, 41, 11251-11256; J. Med. Chem. 2003, 46, 25, 5416-5427; WO 2008/108602 Al or J. Med. Chem. 1998, 41, 13, 2390-2410), by reacting a compound of general formula [XIII] with hydrazine or a hydrated form thereof.
Inert solvent such as cyclic or acyclic ethers (e.g. diethyl ether, tetrahydrofuran, dioxane), alcohols (e.g. methanol or ethanol) can be used.
the reaction can be carried out in mixtures of two or more of these solvents.
a base e.g. triethylamine may be used if desired.
the reaction temperature can be varied from 10°C to 50°C but room temperature is preferred.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but is generally between a couple of minutes and 48 hours.
the reaction can be performed in a microwave apparatus (e.g. CEM Explorer) at elevated temperature, which may shorten the reaction time.
compounds [VII] are removed from the reaction mixture using one of the customary separation techniques. If required, the compounds are purified by recrystallisation, distillation or chromatography.
Compounds of the general formula [VI] may be obtained, according to known literature methods (J. Med. Chem. 1998, 41, 13, 2390-2410), by reacting a compound of general formula [XIII] with hydrazine a hydrazine of general formula R 1 -NH-NH2 or a hydrated form thereof.
Inert solvent such as cyclic or acyclic ethers (e.g. diethyl ether, tetrahydrofuran, dioxane), alcohols (e.g. methanol or ethanol) can be used.
the reaction can be carried out in mixtures of two or more of these solvents.
a base e.g. triethylamine may be used if desired.
the reaction temperature can be varied from 10 °C to 50 °C but room temperature is preferred.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but is generally between a couple of minutes and 48 hours.
the reaction can be performed in a microwave apparatus (e.g. CEM Explorer) at elevated temperature, which may shorten the reaction time.
compounds [VII] are removed from the reaction mixture using one of the customary separation techniques. If required, the compounds are purified by recrystallisation, distillation or chromatography.
the alkynone [XVI] can be prepared by treating acid chloride [XIV] with an alkyne for example by transition-metal catalysis e.g. as described in Tetrahedron Lett. 2006, 47, 5527-5530 or in Synthesis 2003, 18, 2815-2826.
Transition-metal catalysis e.g. as described in Tetrahedron Lett. 2006, 47, 5527-5530 or in Synthesis 2003, 18, 2815-2826.
Compounds of the general formula [XIV] are commercially available or can be synthetised according to known synthesis methods (e.g. in J. Heterocycl. Chem. 1966, 3, 184-186 or J. Med. Chem. 2001, 44, 863-872).
the alkynone [XVI] can be prepared by carbonylative C-C coupling using thiophene halides [XV] with an alkyne employing carbon monoxide as described e.g. in Synlett 2008, 6, 886- 888 or J. Org. Chem. 2005, 70, 6097-6100.
Compounds of formula [VI] can be prepared by treating the alkynone of formula [XVI] with a hydrazine derivative under conditions used in state of the art methodology (e.g. in Eur. J. Org. Chem. 2008, 4157-4168 or Tetrahedron Lett. 2008, 49, 3805-3809).
Compounds of the formula [XIX] can be produced for example by coupling of the pyrazoles of the formula [XVII] (wherein Met stands for a borate ester or boronic acid such as for example B(OiPr)3 or B(OH)2) with compounds of the formula [XVIII] (wherein Z 4 represents a leaving group such as for example CI, Br, I, mesylate or inflate) in the presence of a catalyst, a base, if necessary a ligand and a suitable solvent at suitable temperatures by known literature procedures ⁇ Top. Curr. Chem. 2002, 219, 11 ; Organomet. Chem. 1999, 28, 147 and literature cited therein, Org. Lett.
the processes according to the invention are in general performed under normal pressure. However it is also possible to operate under increased or reduced pressure.
the reaction is in general performed using a blanket gas such as for example argon or nitrogen.
the molar reactant ratio of the pyrazole [XVII] to the compound of the formula [XVIII] is preferably 0.9 to 2.
the catalyst arising as a solid is removed by filtration, the crude product freed from the solvent or solvents and then purified by methods known to those skilled in the art and appropriate for the particular product, e.g. by recrystallization, distillation, sublimation, zone melting, melt crystallization or chromatography.
2-(Trimethylsilyl-ethoxy)methyl and tetrahydropyran-2-yl protective groups can for example be removed in an acidic medium (e.g. with methanolic HCl or trifluoroacetic acid) by known literature procedures (WO 03/099822 and J. Org. Chem. 2008, 73, 4309-4312 and literature contained therein).
Benzylic protective groups can be removed hydrogenolytically with a hydrogen source (e.g. hydrogen, ammonium formate, formic acid or cyclohexene) in the presence of a catalyst (e.g. palladium on activated charcoal or palladium hydroxide on activated charcoal) by known literature procedures (EP-A 1 228 067).
a hydrogen source e.g. hydrogen, ammonium formate, formic acid or cyclohexene
a catalyst e.g. palladium on activated charcoal or palladium hydroxide on activated charcoal
solvent all usual solvents inert under the reaction conditions, such as for example alcohols (e.g. methanol, ethanol, propanol), cyclic and acyclic ethers (e.g. diethyl ether, tetrahydrofuran, dioxan), aromatic hydrocarbons (e.g. benzene, toluene, xylene), halogenated hydrocarbons (e.g. dichloromethane, chloroform, carbon tetrachloride), halogenated aromatic hydrocarbons (e.g. chlorobenzene, dichlorobenzene), nitriles (e.g. acetonitrile), carboxylate ester (e.g.
alcohols e.g. methanol, ethanol, propanol
cyclic and acyclic ethers e.g. diethyl ether, tetrahydrofuran, dioxan
aromatic hydrocarbons e.g. benzen
amides e.g. N,N-dimethylformamide, ⁇ , ⁇ -dimethylacetamide
dimethyl sulphoxide 1,3- dimethyl-2-imidazolinone
water and acetic acid can be used or the reaction can be performed in mixtures of two or more of these solvents.
the reaction is normally performed at temperatures of 0°C - 150°C and preferably at room temperature, but it can also be performed at the reflux temperature of the reaction mixture.
the reaction time varies depending on the scale of the reaction and the reaction temperature, but generally lies between half an hour and 72 hours.
the compounds [XX] are separated from the reaction mixture by one of the usual separation techniques. If necessary, the compounds are purified by recrystallization, distillation or chromatography or if desired can also be used in the next step without prior purification. It is moreover possible to isolate the compound of the general formula [XX] as a salt, e.g. as a salt of hydrochloric acid or trifluoroacetic acid.
a compound of the formula [XXI] is converted into a compound of the formula [XXII] by conversion of the acid into the amide of ⁇ , ⁇ -dimethyl-hydroxylamine by using amide formation methods, which are described in the literature (US 6,562,811 A, US 2003/158185 Al or J. Med. Chem. 2001 , vol. 44, 6, 863-872).
a further subject of the invention relates to an agent for the control of undesired microorganisms and for the reduction of mycotoxins in plants and plant parts, comprising at least one thienylpyri(mi)dinylpyrazole according to the present invention.
the invention relates to a method for the control of undesired microorganisms and for the reduction of mycotoxins in plants and plant parts, characterized in that the thienylpyri(mi)dinylpyrazoles according to the invention are applied onto the microorganisms and/or in their habitat.
the substances according to the invention have potent microbicidal activity and can be employed for controlling unwanted microorganisms, such as fungi and bacteria, in crop protection and in the protection of materials.
the present invention furthermore relates to a crop protection composition for controlling unwanted microorganisms, in particular unwanted fungi, which comprises the active compounds according to the invention.
unwanted microorganisms in particular unwanted fungi
these are preferably fungicidal compositions which comprise agriculturally suitable auxiliaries, solvents, carriers, surfactants or extenders.
the invention relates to a method for controlling unwanted microorganisms, characterized in that the active compounds according to the invention are applied to the phytopathogenic fungi and/or their habitat.
a carrier is a natural or synthetic organic or inorganic substance with which the active compounds are mixed or bonded for better applicability, in particular for application to plants or plant parts or seed.
the carrier which may be solid or liquid, is generally inert and should be suitable for use in agriculture.
Suitable solid or liquid carriers are: for example ammonium salts and ground natural minerals, such as kaolins, clays, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, and ground synthetic minerals, such as finely divided silica, alumina and natural or synthetic silicates, resins, waxes, solid fertilizers, water, alcohols, especially butanol, organic solvents, mineral and vegetable oils and derivatives of these. Mixtures of such carriers may also be used.
ground natural minerals such as kaolins, clays, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth
ground synthetic minerals such as finely divided silica, alumina and natural or synthetic silicates, resins, waxes, solid fertilizers, water, alcohols, especially butanol, organic solvents, mineral and vegetable oils and derivatives of these. Mixtures of such carriers may also be used.
Suitable solid carriers for granules are: for example crushed and fractionated natural rocks such as calcite, marble, pumice, sepiolite, dolomite, and synthetic granules of inorganic and organic meals, and also granules of organic material such as sawdust, coconut shells, maize cobs and tobacco stalks.
Suitable liquefied gaseous extenders or carriers are liquids which are gaseous at ambient temperature and under atmospheric pressure, for example aerosol propellants, such as halogenated hydrocarbons, and also butane, propane, nitrogen and carbon dioxide.
aerosol propellants such as halogenated hydrocarbons, and also butane, propane, nitrogen and carbon dioxide.
Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids such as cephalins and lecithins and synthetic phospholipids can be used in the formulations.
Other possible additives are mineral and vegetable oils. If the extender used is water, it is also possible to employ, for example, organic solvents as auxiliary solvents.
suitable liquid solvents are: aromatics such as xylene, toluene or alkylnaphthalenes, chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or dichloromethane, aliphatic hydrocarbons such as cyclohexane or paraffins, for example mineral oil fractions, mineral and vegetable oils, alcohols such as butanol or glycol and their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethylformamide and dimethyl sulphoxide, and also water.
aromatics such as xylene, toluene or alkylnaphthalenes
chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or dichloromethane
aliphatic hydrocarbons
compositions according to the invention may comprise additional further components, such as, for example, surfactants.
surfactants are emulsifiers and/or foam formers, dispersants or wetting agents having ionic or nonionic properties, or mixtures of these surfactants.
salts of polyacrylic acid salts of lignosulphonic acid, salts of phenolsulphonic acid or naphthalenesulphonic acid, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, substituted phenols (preferably alkylphenols or arylphenols), salts of sulphosuccinic esters, taurine derivatives (preferably alkyl taurates), phosphoric esters of polyethoxylated alcohols or phenols, fatty esters of polyols, and derivatives of the compounds containing sulphates, sulphonates and phosphates, for example alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, arylsulphonates, protein hydrolyzates, lignosulphite waste liquors and methylcellulose.
the presence of a surfactant is required if one of the active compounds and/or one of the inert carriers is insoluble in water and when the application takes place in water.
the proportion of surfactants is between 5 and 40 per cent by weight of the composition according to the invention.
compositions and formulations according to the invention generally comprise between 0.05 and 99% by weight, 0.01 and 98% by weight, preferably between 0.1 and 95% by weight, particularly preferably between 0.5 and 90% of active compound, very particularly preferably between 10 and 70% by weight.
the active compounds or compositions according to the invention can be used as such or, depending on their respective physical and/or chemical properties, in the form of their formulations or the use forms prepared therefrom, such as aerosols, capsule suspensions, cold-fogging concentrates, warm-fogging concentrates, encapsulated granules, fine granules, flowable concentrates for the treatment of seed, ready-to-use solutions, dustable powders, emulsifiable concentrates, oil-in-water emulsions, water-in-oil emulsions, macrogranules, microgranules, oil-dispersible powders, oil-miscible flowable concentrates, oil-miscible liquids, foams, pastes, pesticide coated seed, suspension concentrates, suspoemulsion concentrates, soluble concentrates, suspensions, wettable powders, soluble powders, dusts and granules, water-soluble granules or tablets, water-soluble powders for the treatment of
the formulations mentioned can be prepared in a manner known per se, for example by mixing the active compounds with at least one customary extender, solvent or diluent, emulsifier, dispersant, and/or binder or fixative, wetting agent, water repellent, if appropriate desiccants and UV stabilizers and, if appropriate, dyes and pigments, defoamers, preservatives, secondary thickeners, adhesives, gibberellins and also further processing auxiliaries.
compositions according to the invention include not only formulations which are already ready for use and can be applied with a suitable apparatus to the plant or the seed, but also commercial concentrates which have to be diluted with water prior to use.
the active compounds according to the invention can be present as such or in their (commercial) formulations and in the use forms prepared from these formulations as a mixture with other (known) active compounds, such as insecticides, attractants, sterilants, bactericides, acaricides, ne- maticides, fungicides, growth regulators, herbicides, fertilizers, safeners and/or semiochemicals.
active compounds such as insecticides, attractants, sterilants, bactericides, acaricides, ne- maticides, fungicides, growth regulators, herbicides, fertilizers, safeners and/or semiochemicals.
the treatment according to the invention of the plants and plant parts with the active compounds or compositions is carried out directly or by action on their surroundings, habitat or storage space using customary treatment methods, for example by dipping, spraying, atomizing, irrigating, evaporating, dusting, fogging, broadcasting, foaming, painting, spreading-on, watering (drenching), drip irrigating and, in the case of propagation material, in particular in the case of seeds, furthermore as a powder for dry seed treatment, a solution for seed treatment, a water-soluble powder for slurry treatment, by incrusting, by coating with one or more coats, etc. It is furthermore possible to apply the active compounds by the ultra-low volume method or to inject the active compound preparation or the active compound itself into the soil.
the invention furthermore includes a method for treating seed.
the invention furthermore relates to seed which has been treated in accordance with one of the methods described in the previous paragraph.
the seeds according to the invention are used in methods for the protection of seed from undesirable microorganisms. In these methods, seed treated with at least one active compound according to the invention is employed.
the active compounds or compositions according to the invention are also suitable for treating seed.
a large part of the damage to crop plants caused by harmful organisms is triggered by the infection of the seed during storage or after sowing both during and after germination of the plant. This phase is particularly critical since the roots and shoots of the growing plant are particularly sensitive, and even small damage may result in the death of the plant. Accordingly, there is great interest in protecting the seed and the germinating plant by using appropriate compositions.
the control of phytopathogenic fungi by treating the seed of plants has been known for a long time and is the subject of continuous improvements. However, the treatment of seed entails a series of problems which cannot always be solved in a satisfactory manner.
the present invention therefore also relates to a method for the protection of seed and germinating plants, from attack by phytopathogenic fungi, by treating the seed with a composition according to the invention.
the invention also relates to the use of the compositions according to the invention for treating seed for protecting the seed and the germinating plant against phytopathogenic fungi.
the invention relates to seed treated with a composition according to the invention for protection against phytopathogenic fungi.
One of the advantages of the present invention is that the particular systemic properties of the active compounds and compositions according to the invention mean that treatment of the seed with these active compounds and compositions not only protects the seed itself, but also the resulting plants after emergence, from phytopathogenic fungi. In this manner, the immediate treatment of the crop at the time of sowing or shortly thereafter can be dispensed with.
the active compounds or compositions according to the invention can be used in particular also for transgenic seed where the plant growing from this seed is capable of expressing a protein which acts against pests.
the active compounds or compositions according to the invention By treating such seed with the active compounds or compositions according to the invention, even by the expression of the, for example, insecticidal protein, certain pests may be controlled.
a further synergistic effect may be observed here, which additionally increases the effectiveness of the protection against attack by pests.
compositions according to the invention are suitable for protecting seed of any plant variety which is employed in agriculture, in the greenhouse, in forests or in horticulture and viticulture.
this takes the form of seed of cereals (such as wheat, barley, rye, triticale, sorghum/millet and oats), maize, cotton, soya beans, rice, potatoes, sunflower, bean, coffee, beet (for example sugar beet and fodder beet), peanut, oilseed rape, poppy, olive, coconut, cacao, sugar cane, tobacco, vegetables (such as tomato, cucumbers, onions and lettuce), turf and ornamentals (see also hereinbelow).
the treatment of the seed of cereals (such as wheat, barley, rye, triticale and oats), maize and rice is of particular importance.
transgenic seed As also described further below, the treatment of transgenic seed with the active compounds or compositions according to the invention is of particular importance.
the heterologous gene in transgenic seed can originate, for example, from microorganisms of the species Bacillus, Rhizobium, Pseudomonas, Serratia, Trichoderma, Clavibacter, Glomus or Gliocladium.
this heterologous gene is from Bacillus sp., the gene product having activity against the European corn borer and/or the Western corn rootworm.
the heterologous gene originates from Bacillus thuringiensis.
the composition according to the invention is applied to the seed either alone or in a suitable formulation.
the seed is treated in a state in which it is stable enough to avoid damage during treatment.
the seed may be treated at any point in time between harvest and sowing.
the seed usually used has been separated from the plant and freed from cobs, shells, stalks, coats, hairs or the flesh of the fruits.
seed which has been harvested, cleaned and dried has been treated, for example, with water and then dried again.
the amount of the composition according to the invention applied to the seed and/or the amount of further additives is chosen in such a way that the germination of the seed is not adversely affected, or that the resulting plant is not damaged. This must be borne in mind in particular in the case of active compounds which can have phytotoxic effects at certain application rates.
compositions according to the invention can be applied directly, i.e. without containing any other components and undiluted. In general, it is preferred to apply the compositions to the seed in the form of a suitable formulation. Suitable formulations and methods for treating seed are known to the person skilled in the art and are described, for example, in the following documents: US 4,272,417 A, US 4,245,432 A, US 4,808,430 A, US 5,876,739 A, US 2003/0176428 Al , WO 2002/080675 Al, WO 2002/028186 A2.
the active compounds which can be used in accordance with the invention can be converted into the customary seed-dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries or other coating compositions for seed, and also ULV formulations.
formulations are prepared in a known manner, by mixing the active compounds with customary additives such as, for example, customary extenders and also solvents or diluents, colorants, wetting agents, dispersants, emulsifiers, antifoams, preservatives, secondary thickeners, adhesives, gibberellins and also water.
customary additives such as, for example, customary extenders and also solvents or diluents, colorants, wetting agents, dispersants, emulsifiers, antifoams, preservatives, secondary thickeners, adhesives, gibberellins and also water.
Colorants which may be present in the seed-dressing formulations which can be used in accordance with the invention are all colorants which are customary for such purposes.
pigments which are sparingly soluble in water, but also dyes, which are soluble in water, may be used. Examples which may be mentioned are the colorants known by the names Rhodamin B, C.I. Pigment Red 112 and C.I. Solvent Red 1.
Suitable wetting agents which may be present in the seed-dressing formulations which can be used in accordance with the invention are all substances which promote wetting and which are conventionally used for the formulation of agrochemical active compounds. Preference is given to using alkylnaphthalenesulphonates, such as diisopropyl- or diisobutylnaphthalenesulphonates.
Suitable dispersants and/or emulsifiers which may be present in the seed-dressing formulations which can be used in accordance with the invention are all nonionic, anionic and cationic dispersants conventionally used for the formulation of agrochemical active compounds. Preference is given to using nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants.
Suitable nonionic dispersants which may be mentioned are, in particular, ethylene oxide/propylene oxide block polymers, alkylphenol polyglycol ethers and tristryrylphenol polyglycol ether, and their phosphated or sulphated derivatives.
Suitable anionic dispersants are, in particular, lignosulphonates, polyacrylic acid salts and arylsulphonate/formaldehyde condensates.
Secondary thickeners which may be present in the seed-dressing formulations which can be used in accordance with the invention are all substances which can be employed for such purposes in agrochemical compositions. Cellulose derivatives, acrylic acid derivatives, xanthan, modified clays and finely divided silica are preferred.
Adhesives which may be present in the seed-dressing formulations which can be used in accordance with the invention are all customary binders which can be employed in seed-dressing products.
Polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose may be mentioned as being preferred.
the gibberellins are known (cf. R. Wegler "Chemie der convinced für Schweizer- und Schadlingsbekampfungsstoff" [Chemistry of crop protection agents and pesticides], vol. 2, Springer Verlag, 1970, p. 401 -412).
the seed-dressing formulations which can be used in accordance with the invention can be employed for the treatment of a wide range of seed, including the seed of transgenic plants, either directly or after previously having been diluted with water.
additional synergistic effects may also occur in cooperation with the substances formed by expression.
All mixers which can conventionally be employed for the seed-dressing operation are suitable for treating seed with the seed-dressing formulations which can be used in accordance with the invention or with the preparations prepared therefrom by addition of water. Specifically, a procedure is followed during the seed-dressing operation in which the seed is placed into a mixer, the specific desired amount of seed-dressing formulations, either as such or after previously having been diluted with water, is added, and everything is mixed until the formulation is distributed uniformly on the seed. If appropriate, this is followed by a drying process.
the active compounds or compositions according to the invention have a potent microbicidal activity and can be employed for controlling undesirable microorganisms, such as fungi and bacteria, in crop protection and in the protection of materials.
Fungicides can be employed in crop protection for controlling Plasmodiophoromycetes, Oomycetes, Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes and Deuteromycetes.
Bactericides can be employed in crop protection for controlling Pseudomonadaceae, Rhizobiaceae, Enterobacteriaceae, Corynebacteriaceae and Streptomycetaceae.
the fungicidal compositions according to the invention can be used for the curative or protective control of phytopathogenic fungi. Accordingly, the invention also relates to curative and protective methods for controlling phytopathogenic fungi using the active compounds or compositions according to the invention, which are applied to the seed, the plant or plant parts, the fruit or the soil in which the plants grow.
compositions according to the invention for controlling phytopathogenic fungi in crop protection comprise an effective, but non-phytotoxic amount of the active compounds according to the invention.
Effective, but non-phytotoxic amount means an amount of the composition according to the invention which is sufficient to control the fungal disease of the plant in a satisfactory manner or to eradicate the fungal disease completely, and which, at the same time, does not cause any significant symptoms of phytotoxicity.
this application rate may vary within a relatively wide range. It depends on a plurality of factors, for example on the fungus to be controlled, the plant, the climatic conditions and the ingredients of the compositions according to the invention.
plants are understood here all plants and plant populations such as desired and undesired wild plants or crop plants (including naturally occurring crop plants).
Crop plants can be plants which can be obtained by conventional breeding and optimization methods or by biotechnological and genetic engineering methods or combinations of these methods, including the transgenic plants and including the plant varieties which can or cannot be protected by varietal property rights.
Plant parts are to be understood as meaning all parts and organs of plants above and below the ground, such as shoot, leaf, flower and root, examples which may be mentioned being leaves, needles, stalks, stems, flowers, fruit bodies, fruits, seeds, roots, tubers and rhizomes.
Parts of plants also include harvested plants and vegetative and generative propagation material, for example seedlings, tubers, rhizomes, cuttings and seeds.
the active compounds according to the invention are suitable for the protection of plants and plant organs, for increasing the harvest yields, for improving the quality of the harvested crop, while being well tolerated by plants, having favourable toxicity to warm-blooded species and being environmentally friendly. They may be preferably employed as crop protection agents. They are active against normally sensitive and resistant species and also against all or some stages of development.
plants which can be treated according to the invention cotton, flax, grapevine, fruit, vegetables, such as Rosaceae sp. (for example pome fruits such as apples and pears, but also stone fruits such as apricots, cherries, almonds and peaches, and soft fruits such as strawberries), Ribesioidae sp., Juglandaceae sp., Betulaceae sp., Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae sp., Actinidaceae sp., Lauraceae sp., Musaceae sp.
Rubiaceae sp. for example coffee
Theaceae sp. Sterculiceae sp.
Rutaceae sp. for example lemons, oranges and grapefruit
Solanaceae sp. for example tomatoes
Liliaceae sp. for example lettuce
Umbelliferae sp. for example lettuce
Alliaceae sp. for example leeks, onions
peas for example peas
maj or crop plants such as Gramineae sp. (for example maize, turf, cereals such as wheat, rye, rice, barley, oats, millet and triticale), Poaceae sp. (for example sugar cane), Asteraceae sp. (for example sunflower), Brassicaceae sp. (for example white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, pak choi, kohlrabi, small radishes, and also oilseed rape, mustard, horseradish and cress), Fabacae sp. (for example beans, peanuts), Papilionaceae sp. (for example soya beans), Solanaceae sp.
Gramineae sp. for example maize, turf, cereals such as wheat, rye, rice, barley, oats, millet and triticale
Poaceae sp. for example sugar cane
plants of the plant cultivars which are in each case commercially available or in use are treated according to the invention.
Plant cultivars are to be understood as meaning plants having new properties ("traits") and which have been obtained by conventional breeding, by mutagenesis or by recombinant DNA techniques. They can be cultivars, varieties, bio- or genotypes.
the method of treatment according to the invention can be used in the treatment of genetically modified organisms (GMOs), e.g. plants or seeds.
GMOs genetically modified organisms
Genetically modified plants are plants in which a heterologous gene has been stably integrated into the genome.
the expression "heterologous gene” essentially means a gene which is provided or assembled outside the plant and when introduced in the nuclear, chloroplastic or mitochondrial genome gives the transformed plant new or improved agronomic or other properties by expressing a protein or polypeptide of interest or by downregulating or silencing other gene(s) which are present in the plant (using for example antisense technology, cosuppression technology or RNAi technology [RNA interference]).
a heterologous gene that is located in the genome is also called a transgene.
a transgene that is defined by its particular location in the plant genome is called a transformation or transgenic event.
the treatment according to the invention may also result in superadditive ("synergistic") effects.
superadditive the following effects which exceed the effects which were actually to be expected: reduced application rates and/or a widening of the activity spectrum and/or an increase in the activity of the active compounds and compositions which can be used according to the invention, better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, increased flowering performance, easier harvesting, accelerated maturation, higher harvest yields, bigger fruits, larger plant height, greener leaf colour, earlier flowering, higher quality and/or a higher nutritional value of the harvested products, higher sugar concentration within the fruits, better storage stability and/or processability of the harvested products.
the active compounds according to the invention may also have a strengthening effect on plants. Accordingly, they are suitable for mobilizing the defence system of the plant against attack by unwanted phytopathogenic fungi and/or microorganisms and/or viruses. This may, if appropriate, be one of the reasons for the enhanced activity of the combinations according to the invention, for example against fungi.
Plant-strengthening (resistance-inducing) substances are to be understood as meaning, in the present context, also those substances or combinations of substances which are capable of stimulating the defence system of plants in such a way that, when subsequently inoculated with unwanted phytopathogenic fungi, the treated plants display a substantial degree of resistance to these unwanted phytopathogenic fungi.
the substances according to the invention can be employed for protecting plants against attack by the abovementioned pathogens within a certain period of time after the treatment.
the period within which protection is brought about generally extends from 1 to 10 days, preferably 1 to 7 days, after the treatment of the plants with the active compounds.
Plants and plant varieties which are preferably treated according to the invention include all plants which have genetic material which imparts particularly advantageous, useful traits to these plants (whether obtained by breeding and/or biotechnological means). Plants and plant varieties which are also preferably treated according to the invention are resistant against one or more biotic stress factors, i.e. said plants have a better defence against animal and microbial pests, such as against nematodes, insects, mites, phytopathogenic fungi, bacteria, viruses and/or viroids.
Plants and plant varieties which may also be treated according to the invention are those plants which are resistant to one or more abiotic stress factors.
Abiotic stress conditions may include, for example, drought, cold temperature exposure, heat exposure, osmotic stress, waterlogging, increased soil salinity, increased exposure to minerals, exposure to ozone, exposure to strong light, limited availability of nitrogen nutrients, limited availability of phosphorus nutrients or shade avoidance.
Plants and plant varieties which may also be treated according to the invention are those plants characterized by enhanced yield characteristics. Enhanced yield in said plants can be the result of, for example, improved plant physiology, growth and development, such as water use efficiency, water retention efficiency, improved nitrogen use, enhanced carbon assimilation, improved photosynthesis, increased germination efficiency and accelerated maturation.
Yield can furthermore be affected by improved plant architecture (under stress and non-stress conditions), including early flowering, flowering control for hybrid seed production, seedling vigour, plant size, internode number and distance, root growth, seed size, fruit size, pod size, pod or ear number, seed number per pod or ear, seed mass, enhanced seed filling, reduced seed dispersal, reduced pod dehiscence and lodging resistance.
Further yield traits include seed composition, such as carbohydrate content, protein content, oil content and composition, nutritional value, reduction in anti-nutritional compounds, improved processability and better storage stability.
Plants that may be treated according to the invention are hybrid plants that already express the characteristics of heterosis, or hybrid effect, which results in generally higher yield, vigour, health and resistance towards biotic and abiotic stress factors. Such plants are typically made by crossing an inbred male-sterile parent line (the female parent) with another inbred male-fertile parent line (the male parent). Hybrid seed is typically harvested from the male-sterile plants and sold to growers. Male-sterile plants can sometimes (e.g. in corn) be produced by detasseling (i.e. the mechanical removal of the male reproductive organs or male flowers) but, more typically, male sterility is the result of genetic determinants in the plant genome.
detasseling i.e. the mechanical removal of the male reproductive organs or male flowers
male fertility in hybrid plants which contain the genetic determinants responsible for male sterility
This can be accomplished by ensuring that the male parents have appropriate fertility restorer genes which are capable of restoring the male fertility in hybrid plants that contain the genetic determinants responsible for male sterility.
Genetic determinants for male sterility may be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) were for instance described for Brassica species. However, genetic determinants for male sterility can also be located in the nuclear genome. Male-sterile plants can also be obtained by plant biotechnology methods such as genetic engineering.
a particularly useful means of obtaining male-sterile plants is described in WO 89/10396 in which, for example, a ribonuclease such as a barnase is selectively expressed in the tapetum cells in the stamens. Fertility can then be restored by expression in the tapetum cells of a ribonuclease inhibitor such as barstar.
Plants or plant varieties obtained by plant biotechnology methods such as genetic engineering which may be treated according to the invention are herbicide-tolerant plants, i.e. plants made tolerant to one or more given herbicides. Such plants can be obtained either by genetic transformation, or by selection of plants containing a mutation imparting such herbicide tolerance.
Herbicide-tolerant plants are for example glyphosate-tolerant plants, i.e. plants made tolerant to the herbicide glyphosate or salts thereof.
glyphosate-tolerant plants can be obtained by transforming the plant with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
EPSPS 5-enolpyruvylshikimate-3-phosphate synthase
EPSPS 5-enolpyruvylshikimate-3-phosphate synthase
EPSPS genes are the AroA gene (mutant CT7) of the bacterium Salmonella typhimurium, the CP4 gene of the bacterium Agrobacterium sp., the genes encoding a petunia EPSPS, a tomato EPSPS, or an Eleusine EPSPS. It can also be a mutated EPSPS.
Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate oxidoreductase enzyme. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate acetyltransferase enzyme. Glyphosate-tolerant plants can also be obtained by selecting plants containing naturally occurring mutations of the abovementioned genes. Other herbicide-resistant plants are for example plants which have been made tolerant to herbicides inhibiting the enzyme glutamine synthase, such as bialaphos, phosphinothricin or glufosinate.
Such plants can be obtained by expressing an enzyme detoxifying the herbicide or a mutant glutamine synthase enzyme that is resistant to inhibition.
One such efficient detoxifying enzyme is, for example, an enzyme encoding a phosphinothricin acetyltransferase (such as the bar or pat protein from Streptomyces species for example). Plants expressing an exogenous phosphinothricin acetyltransferase have been described.
hydroxyphenylpyruvatedioxygenase HPPD
Hydroxyphenylpyruvatedioxygenases are enzymes that catalyse the reaction in which para-hydroxyphenylpyruvate (HPP) is transformed into homogentisate.
Plants tolerant to HPPD inhibitors can be transformed with a gene encoding a naturally occurring resistant HPPD enzyme, or a gene encoding a mutated HPPD enzyme.
Tolerance to HPPD inhibitors can also be obtained by transforming plants with genes encoding certain enzymes enabling the formation of homogentisate despite the inhibition of the native HPPD enzyme by the HPPD inhibitor.
Tolerance of plants to HPPD inhibitors can also be improved by transforming plants with a gene encoding an enzyme prephenate dehydrogenase in addition to a gene encoding an HPPD-tolerant enzyme.
ALS inhibitors include, for example, sulphonylurea, imidazolinone, triazolopyrimidines, pyrimidinyl oxy(thio)benzoates, and/or sulphonylaminocarbonyltriazolinone herbicides.
Different mutations in the ALS enzyme also known as acetohydroxy acid synthase, AHAS
AHAS acetohydroxy acid synthase
plants tolerant to imidazolinone and/or sulphonylurea can be obtained by induced mutagenesis, by selection in cell cultures in the presence of the herbicide or by mutation breeding.
Plants or plant varieties obtained by plant biotechnology methods such as genetic engineering which may also be treated according to the invention are insect-resistant transgenic plants, i.e. plants made resistant to attack by certain target insects. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such insect resistance.
insect-resistant transgenic plant includes any plant containing at least one transgene comprising a coding sequence encoding: 1) an insecticidal crystal protein from Bacillus thuringiensis or an insecticidal portion thereof, such as the insecticidal crystal proteins listed online at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/, or insecticidal portions thereof, for example proteins of the Cry protein classes Cryl Ab, Cryl Ac, CrylF, Cry2Ab, Cry3Ae or Cry3Bb or insecticidal portions thereof; or
a crystal protein from Bacillus thuringiensis or a portion thereof which is insecticidal in the presence of a second other crystal protein from Bacillus thuringiensis or a portion thereof, such as the binary toxin made up of the Cy34 and Cy35 crystal proteins; or
a hybrid insecticidal protein comprising parts of two different insecticidal crystal proteins from Bacillus thuringiensis, such as a hybrid of the proteins of 1) above or a hybrid of the proteins of 2) above, for example the Cryl A.1 05 protein produced by maize event MON98034 (WO 2007/027777); or
VIP vegetative insecticidal proteins
a secreted protein from Bacillus thuringiensis or Bacillus cereus which is insecticidal in the presence of a second secreted protein from Bacillus thuringiensis or B. cereus, such as the binary toxin made up of the VTPl A and VIP2A proteins; 7) a hybrid insecticidal protein comprising parts from different secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as a hybrid of the proteins in 1) above or a hybrid of the proteins in 2) above; or
8) a protein of any one of points 1) to 3) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes induced in the encoding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in cotton event COT 102.
insect-resistant transgenic plants also include any plant comprising a combination of genes encoding the proteins of any one of the above classes 1 to 8.
an insect-resistant plant contains more than one transgene encoding a protein of any one of the above classes 1 to 8, to expand the range of target insect species affected or to delay insect resistance development to the plants, by using different proteins insecticidal to the same target insect species but having a different mode of action, such as binding to different receptor binding sites in the insect.
Plants or plant varieties which may also be treated according to the invention are tolerant to abiotic stress factors. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such stress resistance.
Particularly useful stress-tolerant plants include the following: a. plants which contain a transgene capable of reducing the expression and/or the activity of the poly(ADP-ribose)polymerase (PARP) gene in the plant cells or plants; b. plants which contain a stress tolerance-enhancing transgene capable of reducing the expression and/or the activity of the PARG-encoding genes of the plants or plant cells; c.
PARP poly(ADP-ribose)polymerase
plants which contain a stress tolerance-enhancing transgene coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage biosynthesis pathway including nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyltransferase, nicotinami de adenine dinuc le otide synthetas e or nicotinamide phosphoribosyltransferase.
Plants or plant varieties obtained by plant biotechnology methods such as genetic engineering which may also be treated according to the invention show altered quantity, quality and/or storage stability of the harvested product and/or altered properties of specific ingredients of the harvested product such as, for example:
Transgenic plants which synthesize a modified starch which is altered with respect to its chemophysical traits, in particular the amylose content or the amylose/amylopectin ratio, the degree of branching, the average chain length, the distribution of the side chains, the viscosity behaviour, the gel resistance, the grain size and/or grain morphology of the starch in comparison to the synthesized starch in wild-type plant cells or plants, such that this modified starch is better suited for certain applications.
chemophysical traits in particular the amylose content or the amylose/amylopectin ratio, the degree of branching, the average chain length, the distribution of the side chains, the viscosity behaviour, the gel resistance, the grain size and/or grain morphology of the starch in comparison to the synthesized starch in wild-type plant cells or plants, such that this modified starch is better suited for certain applications.
Transgenic plants which synthesize non-starch carbohydrate polymers or which synthesize non-starch carbohydrate polymers with altered properties in comparison to wild-type plants without genetic modification.
Examples are plants which produce polyfructose, especially of the inulin and levan type, plants which produce alpha- 1,4-glucans, plants which produce alpha- 1,6- branched alpha- 1,4-glucans, and plants producing alternan.
Plants or plant varieties obtained by plant biotechnology methods such as genetic engineering which may also be treated according to the invention are plants, such as cotton plants, with altered fibre characteristics.
Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such altered fibre characteristics and include: a) plants, such as cotton plants, which contain an altered form of cellulose synthase genes; b) plants, such as cotton plants, which contain an altered form of rsw2 or rsw3 homologous nucleic acids; c) plants, such as cotton plants, with an increased expression of sucrose phosphate synthase; d) plants, such as cotton plants, with an increased expression of sucrose synthase; e) plants, such as cotton plants, wherein the timing of the plasmodesmatal gating at the basis of the fibre cell is altered, for example through downregulation of fibre-selective ⁇ -1,3- glucanase; f) plants,
Plants or plant cultivars which may also be treated according to the invention are plants, such as oilseed rape or related Brassica plants, with altered oil profile characteristics.
Such plants can be obtained by genetic transformation or by selection of plants containing a mutation imparting such altered oil characteristics and include: a) plants, such as oilseed rape plants, which produce oil having a high oleic acid content; b) plants, such as oilseed rape plants, which produce oil having a low linolenic acid content; c) plants, such as oilseed rape plants, which produce oil having a low level of saturated fatty acids.
transgenic plants which may be treated according to the invention are plants which comprise one or more genes which encode one or more toxins and are the transgenic plants available under the following trade names: YIELD GARD® (for example maize, cotton, soya beans), KnockOut® (for example maize), BiteGard® (for example maize), BT-Xtra® (for example maize), StarLink® (for example maize), Bollgard® (cotton), Nucotn® (cotton), Nucotn 33B® (cotton), NatureGard® (for example maize), Protecta® and NewLeaf® (potato).
YIELD GARD® for example maize, cotton, soya beans
KnockOut® for example maize
BiteGard® for example maize
BT-Xtra® for example maize
StarLink® for example maize
Bollgard® cotton
Nucotn® cotton
Nucotn 33B® cotton
NatureGard® for example maize
herbicide-tolerant plants examples include maize varieties, cotton varieties and soya bean varieties which are available under the following trade names: Roundup Ready® (tolerance to glyphosate, for example maize, cotton, soya beans), Liberty Link® (tolerance to phosphinothricin, for example oilseed rape), IMI® (tolerance to imidazolinone) and SCS® (tolerance to sulphonylurea, for example maize).
Herbicide-resistant plants plants bred in a conventional manner for herbicide tolerance
Clearfield® for example maize.
transgenic plants which may be treated according to the invention are plants containing transformation events, or a combination of transformation events, and that are listed for example in the databases for various national or regional regulatory agencies (see for example http ://gmoinfo.jrc. ec. europa. eu/).
the active compounds or compositions according to the invention can be employed for protecting industrial materials against attack and destruction by unwanted microorganisms, such as, for example, fungi and insects.
the compounds according to the invention can be used alone or in combinations with other active compounds as antifouling compositions.
Industrial materials in the present context are understood as meaning non-living materials which have been prepared for use in industry.
industrial materials which are intended to be protected by active compounds according to the invention from microbial change or destruction can be adhesives, sizes, paper, wallpaper, and board, textiles, carpets, leather, wood, paints and plastic articles, cooling lubricants and other materials which can be infected with, or destroyed by, microorganisms.
Parts of production plants and buildings, for example cooling-water circuits, cooling and heating systems and ventilation and air-conditioning units, which may be impaired by the proliferation of microorganisms may also be mentioned within the scope of the materials to be protected.
Industrial materials which may be mentioned within the scope of the present invention are preferably adhesives, sizes, paper and board, leather, wood, paints, cooling lubricants and heat- transfer liquids, particularly preferably wood.
the active compounds or compositions according to the invention may prevent disadvantageous effects, such as rotting, decay, discoloration, decoloration or formation of mould.
the compounds according to the invention can be employed for protecting objects which come into contact with saltwater or brackish water, in particular hulls, screens, nets, buildings, moorings and signalling systems, against fouling.
the method according to the invention for controlling unwanted fungi can also be employed for protecting storage goods.
storage goods are to be understood as meaning natural substances of vegetable or animal origin or processed products thereof of natural origin, for which long-term protection is desired.
Storage goods of vegetable origin such as, for example, plants or plant parts, such as stems, leaves, tubers, seeds, fruits, grains, can be protected freshly harvested or after processing by (pre)drying, moistening, comminuting, grinding, pressing or roasting.
Storage goods also include timber, both unprocessed, such as construction timber, electricity poles and barriers, or in the form of finished products, such as furniture.
Storage goods of animal origin are, for example, hides, leather, furs and hairs.
the active compounds according to the invention may prevent disadvantageous effects, such as rotting, decay, discoloration, decolouration or formation of mould.
pathogens of fungal diseases which can be treated according to the invention may be mentioned by way of example, but not by way of limitation: diseases caused by powdery mildew pathogens, such as, for example, Blumeria species, such as, for example, Blumeria graminis; Podosphaera species, such as, for example, Podosphaera leucotricha; Sphaerotheca species, such as, for example, Sphaerotheca fuliginea; Uncinula species, such as, for example, Uncinula necator; diseases caused by rust disease pathogens, such as, for example, Gymnosporangium species, such as, for example, Gymnosporangium sabinae; Hemileia species, such as, for example, Hemileia vastatrix; Phakopsora species, such as, for example, Phakopsora pachyrhizi and Phakopsora meibomiae; Puccinia species, such as, for example
Phytophthora species such as, for example, Phytophthora infestans
Plasmopara species such as, for example, Plasmopara viticola
Pseudoperonospora species such as, for example, Pseudoperonospora humuli or Pseudoperonospora cubensis
Pythium species such as, for example, Pythium ultimum
Cercospora species such as, for example, Cercospora beticola
Cladiosporium species such as, for example, Cladiosporium cucumerinum
Cochliobolus species such as, for example, Cochliobolus sativus (conidia form: Drechslera, syn: Helminthosporium) or Cochli
Urocystis species such as, for example, Urocystis occulta
Ustilago species such as, for example, Ustilago nuda, U. nuda tritici
Botrytis species such as, for example, Botrytis cinerea
Penicillium species such as, for example, Penicillium expansum and Penicillium purpurogenum
Sclerotinia species such as, for example, Sclerotinia sclerotiorum
Sclerotinia species such as, for example, Sclerotinia sclerotiorum
Verticilium species such as, for example, Verticilium alboatrum; seed- and soil-borne rot and wilt diseases, and also diseases of seedlings, caused, for example, by Alternaria species, such as, for example, Alternaria brassicicola; Aphanomyces species, such as, for example, Aphanomyces euteiches; Ascochyta species, such as, for example, Ascochyta lentis; Aspergillus species, such as, for example, Aspergillus flavus; Cladosporium species, such as, for example, Cladosporium herbarum; Cochliobolus species, such as, for example, Cochliobolus sativus (conidia form: Drechslera, Bipolaris Syn: Helminthosporium); Colletotrichum species, such as, for example, Colletotrichum coccodes; Fusarium species, such as, for example, Fusarium culmorum; Gibberella species,
Verticillium species such as, for example, Verticillium dahliae
cancerous diseases, galls and witches' broom caused, for example, by Nectria species, such as, for example, Nectria galligena
wilt diseases caused, for example, by Monilinia species, such as, for example, Monilinia laxa
deformations of leaves, flowers and fruits caused, for example, by Exobasidium species, such as, for example, Exobasidium vexans
Taphrina species such as, for example, Taphrina deformans
degenerative diseases of woody plants caused, for example, by Esca species such as, for example, Phaeomoniella chlamydospora, Phaeoacremonium aleophilum or Fomitiporia mediterranea
Ganoderma species such as, for example, Ganoderma boninense
diseases of flowers and seeds caused, for example, by Botrytis species,
Pseudomonas species such as, for example, Pseudomonas syringae pv. lachrymans
Erwinia species such as, for example, Erwinia amylovora.
phytophthora rot (Phytophthora megasperma), brown stem rot (Phialophora gregata), pythium rot (Pythium aphanidermatum, Pythium irregulare, Pythium debaryanum, Pythium myriotylum, Pythium ultimum), rhizoctonia root rot, stem decay, and damping-off (Rhizoctonia solani), sclerotinia stem decay (Sclerotinia sclerotiorum), sclerotinia southern blight (Sclerotinia rolfsii), thielaviopsis root rot (Thielaviopsis basicola).
Microorganisms capable of degrading or changing the industrial materials are, for example, bacteria, fungi, yeasts, algae and slime organisms.
the active compounds according to the invention preferably act against fungi, in particular moulds, wood-discoloring and wood-destroying fungi (Basidiomycetes), and against slime organisms and algae.
Microorganisms of the following genera may be mentioned as examples: Alternaria, such as Alternaria tenuis; Aspergillus, such as Aspergillus niger; Chaetomium, such as Chaetomium globosum; Coniophora, such as Coniophora puetana; Lentinus, such as Lentinus tigrinus; Penicillium, such as Penicillium glaucum; Polyporus, such as Polyporus versicolor; Aureobasidium, such as Aureobasidium pullulans; Sclerophoma, such as Sclerophoma pityophila; Trichoderma, such as Trichoderma viride; Escherichia, such as Escherichia coli; Pseudomonas, such as Pseudomonas aeruginosa; Staphylococcus, such as Staphylococcus aureus.
Alternaria such as Alternaria tenuis
the active compounds according to the invention also have very good antimycotic activity. They have a very broad antimycotic activity spectrum, in particular against dermatophytes and yeasts, moulds and diphasic fungi (for example against Candida species, such as Candida albicans, Candida glabrata), and Epidermophyton floccosum, Aspergillus species, such as Aspergillus niger and Aspergillus fumigatus, Trichophyton species, such as Trichophyton mentagrophytes, Microsporon species such as Microsporon canis and audouinii.
Candida species such as Candida albicans, Candida glabrata
Epidermophyton floccosum Aspergillus species, such as Aspergillus niger and Aspergillus fumigatus
Trichophyton species such as Trichophyton mentagrophytes
Microsporon species such as Microsporon canis and audouinii.
the list of these fungi by no means limits the
the active compounds according to the invention can be used both in medical and in non-medical applications.
the application rates can be varied within a relatively wide range, depending on the kind of application.
the application rate of the active compounds according to the invention is
the combination according to the invention can be used in order to protect plants within a certain time range after the treatment against pests and/or phytopathogenic fungi and/or microorganisms.
the time range, in which protection is effected spans in general 1 to 28 days, preferably 1 to 14 days, more preferably 1 to 10 days, even more preferably 1 to 7 days after the treatment of the plants with the combinations or up to 200 days after the treatment of plant propagation material.
combinations and compositions according to the invention may also be used to reduce the contents of mycotoxins in plants and the harvested plant material and therefore in foods and animal feed stuff made therefrom.
mycotoxins can be specified: Deoxynivalenole (DON), Nivalenole, 15-Ac-DON, 3-Ac-DON, T2- und HT2- Toxins, Fumonisines, Zearalenone Moniliformine, Fusarine, Diaceotoxyscirpenole (DAS), Beauvericine, Enniatine, Fusaroproliferine, Fusarenole, Ochratoxines, Patuline, Ergotalkaloides und Aflatoxines, which are caused for example by the following fungal diseases: Fusarium spec, like Fusarium acuminatum, F. avenaceum, F. crookwellense, F.
Sarcomastigophora (Rhizopoda) wie Entamoebidae z.B. Entamoeba histolytica, Hartmanellidae z.B. Acanthamoeba sp., Harmanella sp.
Apicomplexa (Sporozoa) wie Eimeridae z.B. Eimeria acervulina, E. adenoides, E. alabahmensis, E. anatis, E. anserina, E. arloingi, E. ashata, E. auburnensis, E. bovis, E. brunetti, E. canis, E. chinchillae, E. clupearum, E. columbae, E. contorta, E. crandalis, E. debliecki, E. dispersa, E. ellipsoidales, E. falciformis, E. faurei, E. flavescens, E.
Theileria spec wie Adeleina z.B. Hepatozoon canis, H. spec.
the crude mixture is filtered through a cartridge with Celite and the volatile components removed under vacuum.
the crude product is purified by chromatography on silica gel (cyclohexane /ethyl acetate) and 26.2 mg of 4-[5-(5-methyl-3-thienyl)-l-(tetrahydro-2H-pyran-2-yl)-lH-pyrazol-4-yl]pyridine (41%) are obtained as a colourless oil.
the intermediates produced in the general procedure VI 3 can also be used in the deprotection reaction without further purification.
the protected thienylpyrazole [XIX-1] (26.2mg, 0.08mmol) crude product is dissolved in 2 mL methanol and treated with 800 ⁇ of HC1 in dioxane (4 molar). The solution is stirred at room temperature for lhr and then concentrated. The solid obtained is washed with aqueous sodium hydrogen carbonate solution and extracted with ethyl acetate. After drying and evaporation of the solvent 25.6 mg of 4-[3-(5-methyl-3-thienyl)-lH-pyrazol-4-yl]pyridine (54%) are obtained as a white solid.
the combined organic phases are washed with water (70 mL) and brine, dried over magnesium sulphate and concentrated to give the crude product as a yellow oil.
the crude product is purified by silica gel chromatography (eluent: dichloromethane/methyl tert-butyl ether) to yield 590 mg (59%) of 3-(2,5-dimethyl-3-thienyl)-l- isopropyl-lH-pyrazole as a yellowish oil and 300 mg (30%) of 5-(2,5-dimethyl-3-thienyl)-l - isopropyl-lH-pyrazole as a yellow oil.
Aqueous 5% sodium thiosulfate (150 mL) and saturated aqueous sodium carbonate solution (100 mL) are added and the mixture is stirred at room temperature for 1 h, then extracted with MTBE (3 x 120 mL). The combined organic phases are washed with water (70 mL) and brine, dried over magnesium sulphate and concentrated to give the crude product as a light brown oil.
1,4-Dioxane and aqueous 2M sodium carbonate solution are degassed by bubbling a flow of argon through the solutions for 10 min.
2.06 g 4-iodo-l-isopropyl-3-(3-thienyl)-lH-pyrazole (6.15 mmol) are weighed into a 100 mL three-necked round bottom flask equipped with a reflux condenser and an internal thermometer.
reaction mixture is evaporated under vacuum and purified by silica gel chromatography (eluent: cyclohexane/ethyl acetate) to yield 1.69 g (72%) of the intermediate tert-butyl ⁇ 4-[l - isopropyl-3-(3-thienyl)-lH-pyrazol-4-yl]pyridin-2-yl ⁇ carbamate as a white solid.
1,4-Dioxane and aqueous 2M sodium carbonate solution are degassed by bubbling a flow of argon through the solutions for 10 min.
167.0 mg 3-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)pyridine (0.75 mmol) and 36.6 mg l, -bis(diphenylphosphino)-ferrocene]dichloropalladium(II) (0.05 mmol) are weighed into a microwave vial (2-5 mL reaction volume).
cyclopropanecarbonyl chloride (1.94 mmol) is added dropwise to a solution of 183.4 mg 4-[l-isopropyl-3-(3-thienyl)-lH-pyrazol-4-yl]pyridin-2-amine (0.65 mmol) and 0.27 mL triethylamine (1.94 mmol) in dichloromethane (2.5 mL). The mixture is stirred at room temperature overnight and diluted with 2.5 mL dichloromethane.
the stated logP value is the value for the stated pure compound or, if a mixture is present, for the main isomer (1 -substituted 3-aryl-lH-pyrazole).
the mass detection was effected with the Micronass ZQ2000 mass detector from Waters.
Method C Note on the determination of the logP values and mass detection: The stated logP values were determined in accordance with EEC-Directive 79/83 1 Annex V.A8 by UPLC (Ultra Performance Liquid Chromatography) on a reverse phase column (CI 8). HP1100; 50*2.1 Zorbax Eclipse Plus C18 1.8 micron; eluent A: acetonitrile (0.09% formic acid); eluent B: water (0.1% formic acid); linear gradient from 10% A to 95% A in 3.25 min; oven temperature 40°C; flow rate: 0.8 mL/min.
the mass detection was effected with the LCT Premier or SQD mass detector from Waters.
Emulsifier 1 part by weight of Alkylarylpolyglycolether
active compound 1 part by weight of active compound is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
Emulsifier 1 part by weight of Alkylarylpolyglycolether To produce a suitable preparation of active compound, 1 part by weight of active compound is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
the test is evaluated 7 days after the inoculation. 0% means an efficacy which corresponds to that of the untreated control, while an efficacy of 100% means that no disease is observed.
Emulsifier 1 part by weight of Alkylarylpolyglycolether To produce a suitable preparation of active compound, 1 part by weight of active compound is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
the test is evaluated 7-9 days after the inoculation. 0%> means an efficacy which corresponds to that of the untreated control while an efficacy of 100%) means that no disease is observed.
Emulsifier 1 part by weight of Alkylarylpolyglycolether
active compound 1 part by weight of active compound is mixed with the stated amounts of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.
the test is evaluated 7 days after the inoculation. 0%> means an efficacy which corresponds to that of the untreated control while an efficacy of 100%) means that no disease is observed.
the following compounds according to the invention showed efficacy of 70% or even higher at a concentration of 500ppm of active ingredient: 1 (95%), 6(95%), 7(90%), 8(80%), 9(95%), 10(95%), 11(70%), 12(95%), 16(90%), 18(80%), 19(95%), 21 (70%), 22(95%), 30(90%), 27(80%), 28(95%), 26(90%), 23(80%), 24(80%), 25(70%), 31(90).

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