WO2012161502A3 - 실리카 캡슐화된 단분자 나노효소입자 제조방법 및 이를 통해 제조된 단분자 나노효소입자 - Google Patents

실리카 캡슐화된 단분자 나노효소입자 제조방법 및 이를 통해 제조된 단분자 나노효소입자 Download PDF

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Publication number
WO2012161502A3
WO2012161502A3 PCT/KR2012/004053 KR2012004053W WO2012161502A3 WO 2012161502 A3 WO2012161502 A3 WO 2012161502A3 KR 2012004053 W KR2012004053 W KR 2012004053W WO 2012161502 A3 WO2012161502 A3 WO 2012161502A3
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WO
WIPO (PCT)
Prior art keywords
enzyme nanoparticles
nanoparticles
manufacturing
enzyme
mannufactured
Prior art date
Application number
PCT/KR2012/004053
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English (en)
French (fr)
Other versions
WO2012161502A2 (ko
Inventor
김정배
홍성길
이병수
Original Assignee
고려대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고려대학교 산학협력단 filed Critical 고려대학교 산학협력단
Priority to US14/112,834 priority Critical patent/US9458448B2/en
Publication of WO2012161502A2 publication Critical patent/WO2012161502A2/ko
Publication of WO2012161502A3 publication Critical patent/WO2012161502A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/06—Making microcapsules or microballoons by phase separation
    • B01J13/14—Polymerisation; cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Silicon Compounds (AREA)

Abstract

본 입자의 실리카 캡슐화를 통한 단분자 나노효소입자 제조방법은 종래의 단분자 효소 나노입자 합성과정에서 표면작용기화 과정과 고분자화 과정을 포함하지 않으며, 반응조건이 마일드하여 대량생산에 적합하다.
PCT/KR2012/004053 2011-05-23 2012-05-23 실리카 캡슐화된 단분자 나노효소입자 제조방법 및 이를 통해 제조된 단분자 나노효소입자 WO2012161502A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/112,834 US9458448B2 (en) 2011-05-23 2012-05-23 Manufacturing method for silica encapsulated single-enzyme nanoparticles and single-enzyme nanoparticles manufactured by means of method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0048437 2011-05-23
KR20110048437 2011-05-23

Publications (2)

Publication Number Publication Date
WO2012161502A2 WO2012161502A2 (ko) 2012-11-29
WO2012161502A3 true WO2012161502A3 (ko) 2013-03-21

Family

ID=47217892

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/004053 WO2012161502A2 (ko) 2011-05-23 2012-05-23 실리카 캡슐화된 단분자 나노효소입자 제조방법 및 이를 통해 제조된 단분자 나노효소입자

Country Status (3)

Country Link
US (1) US9458448B2 (ko)
KR (1) KR101411793B1 (ko)
WO (1) WO2012161502A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016041488A1 (en) * 2014-09-19 2016-03-24 The Hong Kong University Of Science And Technology Antimicrobial coating for long-term disinfection of surfaces
GB2552704A (en) * 2016-08-04 2018-02-07 Univ Bath Biomolecule preservation
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
CN110006888A (zh) * 2019-05-10 2019-07-12 中国农业大学 一种基于单原子纳米酶的抗坏血酸快速检测试纸
KR20200142870A (ko) 2019-06-13 2020-12-23 한국세라믹기술원 다공성 탄산칼슘 마이크로스피어를 이용한 효소 고정화 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100472910B1 (ko) 2001-02-01 2005-03-08 주식회사 고센바이오텍 역미셀을 이용하여 난백으로부터 리소자임을 추출하는 방법
KR100845008B1 (ko) * 2006-08-09 2008-07-08 한국생명공학연구원 표면에 나노 구멍 또는 기공을 가지는 실리카 캡슐 및 그제조방법
FR2952368B1 (fr) * 2009-11-10 2012-01-13 Commissariat Energie Atomique Particule de silice incorporant une molecule d'interet, son procede de preparation et ses utilisations.

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CAO, A. ET AL.: "A facile method to encapsulate proteins in silica nanoparticles: encapsulated green fluorescent protein as a robust fluorescence probe", ANGEW. CHEM., vol. 122, 22 March 2010 (2010-03-22), pages 3086 - 3089 *
DOMACH, M. ET AL.: "Stabilizing biomacromolecules in nontoxic nano-structured materials", JOURNAL OF ASSOCIATION FOR LABORATORY AUTOMATION, vol. 15, April 2010 (2010-04-01), pages 136 - 144 *
HEGEDUS, I. ET AL.: "Improvement of chymotrypsin enzyme stability as single enzyme nanoparticles", CHEMICAL ENGINEERING SCIENCE, vol. 64, 17 November 2008 (2008-11-17), pages 1053 - 1060 *
HONG, R. ET AL.: "Monolayer-controlled substrate selectively using noncovalent enzyme-nanoparticle conjugates", J. AM. CHEM. SOC., vol. 126, 29 September 2004 (2004-09-29), pages 13572 - 13573 *
KIM, J. ET AL.: "Single enzyme nanoparticles in nanoporous silica: a hierarchical approach to enzyme stabilization and immobilization", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, 3 July 2006 (2006-07-03), pages 474 - 480 *

Also Published As

Publication number Publication date
KR101411793B1 (ko) 2014-06-24
KR20130028630A (ko) 2013-03-19
WO2012161502A2 (ko) 2012-11-29
US20140127778A1 (en) 2014-05-08
US9458448B2 (en) 2016-10-04

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