WO2012097091A3 - Engineered microorganisms with enhanced fermentation activity - Google Patents

Engineered microorganisms with enhanced fermentation activity Download PDF

Info

Publication number
WO2012097091A3
WO2012097091A3 PCT/US2012/020982 US2012020982W WO2012097091A3 WO 2012097091 A3 WO2012097091 A3 WO 2012097091A3 US 2012020982 W US2012020982 W US 2012020982W WO 2012097091 A3 WO2012097091 A3 WO 2012097091A3
Authority
WO
WIPO (PCT)
Prior art keywords
fermentation activity
engineered microorganisms
enhanced fermentation
microorganisms
enhanced
Prior art date
Application number
PCT/US2012/020982
Other languages
French (fr)
Other versions
WO2012097091A2 (en
Inventor
Kirsty Anne Lily SALMON
Jose Miguel Laplaza
Stephen Picataggio
Original Assignee
Verdezyne, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verdezyne, Inc. filed Critical Verdezyne, Inc.
Publication of WO2012097091A2 publication Critical patent/WO2012097091A2/en
Publication of WO2012097091A3 publication Critical patent/WO2012097091A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms.
PCT/US2012/020982 2011-01-12 2012-01-11 Engineered microorganisms with enhanced fermentation activity WO2012097091A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161432104P 2011-01-12 2011-01-12
US61/432,104 2011-01-12

Publications (2)

Publication Number Publication Date
WO2012097091A2 WO2012097091A2 (en) 2012-07-19
WO2012097091A3 true WO2012097091A3 (en) 2012-10-26

Family

ID=46507653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/020982 WO2012097091A2 (en) 2011-01-12 2012-01-11 Engineered microorganisms with enhanced fermentation activity

Country Status (1)

Country Link
WO (1) WO2012097091A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2798937A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
US9926347B2 (en) 2013-11-05 2018-03-27 Board Of Regents, The University Of Texas System Methods for engineering sugar transporter preferences
WO2016016805A1 (en) 2014-08-01 2016-02-04 Versalis S.P.A. Gene construct for the transformation of yeast strains
US9920312B2 (en) 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization
US10619147B2 (en) * 2014-08-11 2020-04-14 Lallemand Hungary Liquidity Management Llc Chimeric polypeptides having xylose isomerase activity
US10724015B2 (en) * 2016-05-18 2020-07-28 Korea University Research And Business Foundation Microorganism having improved ability to produce N-acetylglucosamine as a result of modulating glycolytic flux
US11091753B2 (en) 2017-05-31 2021-08-17 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
CN110869488B (en) * 2017-07-11 2024-04-05 安迪苏法国联合股份有限公司 Enhanced metabolite producing yeast
CN114107357B (en) * 2020-08-27 2024-04-05 中国科学院天津工业生物技术研究所 Recombinant filamentous fungus for producing ethanol by phosphofructokinase2 mutant, construction thereof and application of recombinant filamentous fungus in ethanol production
CN114592023B (en) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 Cell lysis self-assembly polypeptide compound, self-assembly method, self-assembly polypeptide preparation and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010039692A2 (en) * 2008-09-30 2010-04-08 The United States Of America, As Represented By The Secretary Of Agriculture Transformed saccharomyces cerevisiae engineered for xylose utilization

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010039692A2 (en) * 2008-09-30 2010-04-08 The United States Of America, As Represented By The Secretary Of Agriculture Transformed saccharomyces cerevisiae engineered for xylose utilization

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI 10 August 2010 (2010-08-10), "RecName: Full=Xylose isomerase", accession no. 9S306 *
DATABASE NCBI 15 April 2005 (2005-04-15), "Ruminococcus flavefaciens xylan utilization operon", accession no. J132472 *
KUYPER, M. ET AL.: "Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain", FEMS YEAST RESEARCH., vol. 5, no. 10, July 2005 (2005-07-01), pages 925 - 834, XP004967640, DOI: doi:10.1016/j.femsyr.2005.04.004 *
KUYPER, M. ET AL.: "HIgh-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae?", FEMS YEAST RESEARCH., vol. 4, no. 1, October 2003 (2003-10-01), pages 69 - 78, XP002312913, DOI: doi:10.1016/S1567-1356(03)00141-7 *
MADHAVAN, A. ET AL.: "Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY., vol. 82, no. 6, 6 June 2009 (2009-06-06), pages 1037 - 1047, XP019705462 *

Also Published As

Publication number Publication date
WO2012097091A2 (en) 2012-07-19

Similar Documents

Publication Publication Date Title
WO2011006126A3 (en) Engineered microorganisms with enhanced fermentation activity
WO2012097091A3 (en) Engineered microorganisms with enhanced fermentation activity
IL273159A (en) Culture medium composition, and method for culturing cell or tissue using said composition
WO2013083999A3 (en) Nano-enhanced elastomers
WO2012014076A3 (en) Liver organoid, uses thereof and culture method for obtaining them
EP2818543B8 (en) Bacillus, hyaluronic acid enzyme, and uses thereof
WO2012166425A3 (en) Methods of amplifying whole genome of a single cell
WO2013052907A3 (en) Methods and processes for non-invasive assessment of genetic variations
WO2010088633A3 (en) Novel cell lines and methods
EP2612922A4 (en) Eggshell membrane solubilization method using enzymes
WO2012051210A3 (en) Mesenchymal stem cells and related therapies
WO2011133504A3 (en) Nanozymes, methods of making nanozymes, and methods of using nanozymes
WO2012149058A3 (en) Methods and system for interfering with viability of bacteria and related compounds and compositions
GB2497059B (en) Culture medium, method for culturing listeria,and method for dtecting listeria
EP2880050A4 (en) Novel enzymes, enzyme components and uses thereof
WO2012082731A3 (en) Engineered microalgae with enhanced lipid production
AU2013225816A8 (en) Compositions and methods for treating Type III Gaucher Disease
WO2012112416A3 (en) Method of producing n-butyraldehyde
WO2012016177A3 (en) Genetically modified microbes producing increased levels of acetyl-coa derived compounds
HK1184823A1 (en) Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
EP2865748A4 (en) Culture medium for bacteria of the genus clostridium without components of animal origin, and method for producing a supernatant containing one or more proteases with colagenolytic and gelatinolytic activity
WO2012021678A3 (en) Isolated bacteria, methods for use, and methods for isolation
EP3049529A4 (en) Engineered enzyme having acetoacetyl-coa hydrolase activity, microorganisms comprising same, and methods of using same
EP2918666A4 (en) Method for fermenting must, and fermentation vat
EP2626430A4 (en) YEAST AND YEAST EXTRACT CONTAINING -Glu-Abu, AND METHOD FOR PRODUCING THE YEAST OR YEAST EXTRACT CONTAINING -Glu-Abu

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12734491

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12734491

Country of ref document: EP

Kind code of ref document: A2